Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [microbiological characterization of listeria monocytogenes isolated from different sources in the primorsky territory]. | the results of the bacteriological study of material, taken from humans, rodents, hydro-bios and environmental objects (including foodstuffs) in the primorsky territory, for the presence of l. monocytogenes bacteria are given. 83 bacterial strains of the genus listeria were isolated. as revealed in this study, 25 of these strains belonged to l. monocytogenes (30.12%), 8 strains--to l. innocua (9.6%), 6 strains--to l. seeligeri (7.2%) and 2 strains--to l. ivanovii (2.4%). the greatest number of l ... | 2006 | 16758889 |
| listeriosis associated with gorgonzola (italian blue-veined cheese). | we describe a case of listeriosis in italy associated with the consumption of cheese. opened samples of two brands of gorgonzola (italian blue-veined cheese; referred to as brands "b" and "c") were collected from the patient's refrigerator. unopened samples of the brand suspected to be the source of infection (brand b) were taken from the store where the cheese had been purchased, other local stores, and the production plant. listeria monocytogenes serotype 1/2b was isolated from the patient and ... | 2006 | 16761945 |
| characterization of a spontaneous, pressure-tolerant listeria monocytogenes scott a ctsr deletion mutant. | a spontaneous, pressure-tolerant mutant of listeria monocytogenes scott a, designated 2-1, was isolated after several rounds of pressure treatments at 500 mpa for 10 min. mutant 2-1 was almost 100,000-fold more resistant than the wild type to a pressure of 350 mpa, and about 100-fold more resistant to 450 mpa when pressurized in growth medium. approximately ten times more mutant cells than wild-type cells survived a 20-min exposure to 55 degrees c, and the mutant appears also to be more resistan ... | 2006 | 16761946 |
| simultaneous assessment of antigen-stimulated cytokine production and memory subset composition of memory cd8 t cells. | the functional identification of antigen-specific cd8 t cell populations is critical to understanding host responses to infection by intracellular pathogens. furthermore, assessing the properties of protective memory cd8 t cell populations generated by immunization is necessary in the rational design of vaccines. recently, a classification scheme was proposed in which memory cd8 t cells were divided into one of two distinct subsets, based on cd62l expression, that have different functional prope ... | 2006 | 16762359 |
| effect of the inoculum size on listeria monocytogenes growth in structured media. | obtaining quantitative data concerning the relative impact of various factors that may influence bacterial growth is of great importance for microbial risk assessment and predictive microbiology. the objective of this work was to investigate the effect of the initial listeria monocytogenes density on all the growth parameters of this pathogen (lag phase duration, growth rate and maximum population density attained) on a sterile solid model system mimicking smoked fishery products, and in real co ... | 2006 | 16762438 |
| low virulence but potentially fatal outcome-listeria ivanovii. | of the six known species of listeria, listeria monocytogenes is classified as the only human pathogen causing listeriosis, a highly fatal, opportunistic infection contracted via food. listeria ivanovii is the only other pathogenic species of the genus and is considered to be specific to ruminants, except for extremely rare cases of infection in humans. we report here on a 64-year-old male patient with l. ivanovii who responded favorably to treatment with intravenous ampicillin. | 2006 | 16762780 |
| recovery of heat-injured listeria innocua. | listeria innocua was subjected to thermal inactivation and the extent of heat-injured cells was quantified. cultures were heated in liquid medium for different times, using temperatures in the range of 52.5 to 65.0 degrees c, and plated on tryptic soy agar with 0.6% yeast extract (tsaye) used as non-selective medium and on tsaye plus 5% nacl (tsaye+nacl) and palcam agar with selective supplement (palcam agar) as selective media. the difference observed in counts in non-selective and in selective ... | 2006 | 16784792 |
| self-antigen maintains the innate antibacterial function of self-specific cd8 t cells in vivo. | self-specific cd8 t cells, which are selected by high-affinity interactions with self-ags, develop into a lineage distinct from conventional cd8 t cells. we have previously shown that these self-specific cells acquire phenotypic and functional similarities to cells of the innate immune system including the expression of functional receptors associated with nk cells. in this study, we show that these self-specific cells have the ability to produce large amounts of ifn-gamma in response to infecti ... | 2006 | 16785508 |
| division of labor and cooperation between dendritic cells. | 2006 | 16785884 | |
| a rapid molecular-based assay for direct quantification of viable bacteria in slaughterhouses. | a rapid test for microbial quantification in carcass and environmental swabs that does not require enrichment and provides results in less than 4 h is described here. steps in the assay include the rapid concentration of bacteria on sponge swabs by vacuum filtration followed by real-time pcr detection. the assay has been applied for the detection of coliforms, escherichia coli o157:h7, salmonella spp., and listeria monocytogenes on carcass swabs and environmental samples in a slaughterhouse-proc ... | 2006 | 16786844 |
| chlorine resistance of listeria monocytogenes biofilms and relationship to subtype, cell density, and planktonic cell chlorine resistance. | strains of listeria monocytogenes vary in their ability to produce biofilms. this research determined if cell density, planktonic chlorine resistance, or subtype are associated with the resistance of l. monocytogenes biofilms to chlorine. thirteen strains of l. monocytogenes were selected for this research based on biofilm accumulation on stainless steel and rep-pcr subtyping. these strains were challenged with chlorine to determine the resistance of individual strains of l. monocytogenes. plank ... | 2006 | 16786848 |
| characterization of listeria monocytogenes isolates from 50 small-scale austrian cheese factories. | one hundred eighty-one small-scale cheese factories (annual production < 100,000 kg) were tested for the presence of listeria monocytogenes in cheese and smear samples from 1997 to 2000. in total, 2615 samples were drawn. fifty (27.6%) of 181 enterprises yielded l. monocytogenes. from 14 of the cheese-making facilities, we obtained more than four l. monocytogenes isolates. a total of 182 mostly cheese- and smear-borne l. monocytogenes strains were characterized by serotyping and pulsed-field gel ... | 2006 | 16786849 |
| contamination of cooked peeled shrimp (pandalus borealis) by listeria monocytogenes during processing at two processing plants. | listeria spp. and listeria monocytogenes contamination was evaluated in cooked peeled shrimp (final or semifinal product, 82 samples) and the shrimp-processing environment (two plants, 613 samples) in eight surveys conducted from 1998 through 2001. listeria was detected in 12.5% (78) of the 695 samples (11.2% of the samples were positive for l. monocytogenes), but none of the samples of final product contained listeria. one hundred seventy-two l. monocytogenes isolates were characterized by puls ... | 2006 | 16786850 |
| determination of the growth limits and kinetic behavior of listeria monocytogenes in a sliced cooked cured meat product: validation of the predictive growth model under constant and dynamic temperature storage conditions. | to describe the growth limits of listeria monocytogenes nctc10527 in a sliced vacuum-packaged cooked cured meat product, the binary logistic regression model was used to develop an equation to determine the probability of growth or no growth of l. monocytogenes as a function of temperature (from 0 to 10 degrees c) and water activity (from 0.88 to 0.98). two inoculum concentrations were used (10 and 10(4) cfu g(-1)), and the growth limits for the two inocula were different. the kinetic behavior o ... | 2006 | 16786851 |
| inhibition of listeria innocua in hummus by a combination of nisin and citric acid. | the effect of nisin or citric acid or combinations of these two inhibitors on the inactivation of a cocktail of three listeria innocua strains was investigated in a model brain heart infusion (bhi) broth and hummus (chickpea dip). in bhi broth, citric acid had a limited ability to inhibit l. innocua growth. nisin initially reduced l. innocua concentrations by about 3 log cycles; however, l. innocua reached concentrations similar to those of the control after 5 days at 22 degrees c. in combinatio ... | 2006 | 16786852 |
| effect of cheese water activity and carbohydrate content on the barotolerance of listeria monocytogenes scott a. | high-pressure processing is an appropriate technique for improving the microbiological safety of packaged ready-to-eat foods. the effect of high-pressure treatment on listeria monocytogenes scott a inoculated into fresh hispánico-type cheese and ripe mahón cheese was investigated. a 3.8-log reduction in the counts of l. monocytogenes scott a in fresh cheese was recorded after 3 min at 400 mpa and 12 degrees c, whereas 18 min under the same conditions was required to obtain a 1-log reduction in r ... | 2006 | 16786853 |
| deoxynivalenol and satratoxin g potentiate proinflammatory cytokine and macrophage inhibitory protein 2 induction by listeria and salmonella in the macrophage. | health risks from microbial pathogens and toxins encountered in food and the environment continue to be of worldwide concern. the purpose of this research was to test the hypothesis that trichothecene mycotoxins amplify inflammatory responses to foodborne bacterial pathogens. we assessed the capacity of deoxynivalenol (don) and satratoxin g (sg) to potentiate chemokine and proinflammatory cytokine production in raw 264.7 murine macrophages induced by listeria monocytogenes and salmonella typhimu ... | 2006 | 16786854 |
| pulsed-field gel electrophoresis typing of listeria monocytogenes isolated in two finnish fish farms. | the aim of this study was to find sources of listeria monocytogenes contamination in fish products from a fish farm. the occurrence of l. monocytogenes also was compared in two freshwater fish farms with different types of fishponds. samples collected from chilled rainbow trout (oncorhynchus mykiss) and the slaughterhouse environment did not contain l. monocytogenes, but listeria innocua was found in two samples from the slaughterhouses. ten isolates of l. monocytogenes were discovered in sedime ... | 2006 | 16786871 |
| cold stress tolerance of listeria monocytogenes: a review of molecular adaptive mechanisms and food safety implications. | the foodborne pathogen listeria monocytogenes has many physiological adaptations that enable survival under a wide range of environmental conditions. the microbes overcome various types of stress, including the cold stress associated with low temperatures in food-production and storage environments. cold stress adaptation mechanisms are therefore an important attribute of l. monocytogenes, enabling these food pathogens to survive and proliferate to reach minimal infectious levels on refrigerated ... | 2006 | 16786878 |
| performance evaluation of a low conductive growth medium (lcgm) for growth of healthy and stressed listeria monocytogenes and other common bacterial species. | the performance of a low conductive growth medium (lcgm) (conductivity of <1300 micros) was evaluated for its ability to support growth of food borne bacterial pathogens including listeria monocytogenes and to determine the expression of the two key virulence proteins in l. monocytogenes for possible applications in an impedance-based microfluidic biochip detection platform. growth of listeria was monitored spectrophotometrically and the lag phase, generation time, growth rate and maximum popula ... | 2006 | 16790285 |
| comparison of different live vaccine strategies in vivo for delivery of protein antigen or antigen-encoding dna and mrna by virulence-attenuated listeria monocytogenes. | listeria monocytogenes can be used to deliver protein antigens or dna and mrna encoding such antigens directly into the cytosol of host cells because of its intracellular lifestyle. in this study, we compare the in vivo efficiencies of activation of antigen-specific cd8 and cd4 t cells when the antigen is secreted by l. monocytogenes or when antigen-encoding plasmid dna or mrna is released by self-destructing strains of l. monocytogenes. infection of mice with self-destructing l. monocytogenes c ... | 2006 | 16790768 |
| surface plasmon resonance biosensors for detection of pathogenic microorganisms: strategies to secure food and environmental safety. | this review describes the exploitation of exclusively optical surface plasmon resonance (spr) biosensors for the direct and indirect detection of pathogenic microorganisms in food chains and the environment. direct detection is, in most cases, facilitated by the use of defined monoclonal or polyclonal antibodies raised against (a part of) the target pathogenic microorganisms. the antibodies were immobilized to a solid phase of the sensor to capture the microbe from the sample. alternatively, ant ... | 2006 | 16792081 |
| biosensors for the analysis of food- and waterborne pathogens and their toxins. | biosensors are devices which combine a biochemical recognition element with a physical transducer. there are various types of biosensors, including electrochemical, acoustical, and optical sensors. biosensors are used for medical applications and for environmental testing. although biosensors are not commonly used for food microbial analysis, they have great potential for the detection of microbial pathogens and their toxins in food. they enable fast or real-time detection, portability, and mult ... | 2006 | 16792089 |
| recovery and characterization of a 30.7-kda protein from bacillus licheniformis associated with inhibitory activity against methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, and listeria monocytogenes. | of 131 bacterial isolates from seaweed, a culture of bacillus licheniformis produced a novel protein with antibacterial activity against methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, and listeria monocytogenes. the antibacterial activity was maximal in cultures prepared in columbia broth containing pieces of synthetic polyurethane sponge and shaken at 210 to 230 rpm. antibacterial activity was not found in cultures grown statically or with different speeds of rot ... | 2006 | 16794953 |
| bioassay-guided purification and identification of antimicrobial components in chinese green tea extract. | the chinese green tea extract was found to strongly inhibit the growth of major food-borne pathogens, escherichia coli o157:h7, salmonella typhimurium dt104, listeria monocytogenes, staphylococcus aureus, and a diarrhoea food-poisoning pathogen bacillus cereus, by 44-100% with the highest activity found against s. aureus and lowest against e. coli o157:h7. a bioassay-guided fractionation technique was used for identifying the principal active component. a simple and efficient reversed-phase high ... | 2006 | 16797571 |
| simultaneous detection by pcr of escherichia coli, listeria monocytogenes and salmonella typhimurium in artificially inoculated wheat grain. | a multiplex pcr procedure was established to detect escherichia coli, listeria monocytogenes and salmonella typhimurium in artificially inoculated wheat grain. the pcr protocol with an enrichment step successfully detected all three organisms inoculated together in non-autoclaved wheat grain. after a one day enrichment, e. coli, l. monocytogenes and s. typhimurium were detected at levels of 56, 1800 and <54 cfu/ml, respectively, in the initial sample. for l. monocytogenes, an improved detection ... | 2006 | 16797761 |
| identification of a cross-reactive hla-drb1*0301-restricted cd4 t cell response directed against cholesterol-binding cytolysins from two different pathogens. | cholesterol-binding cytolysins constitute an evolutionarily conserved family of pore-forming proteins expressed by different gram-positive pathogens. listeriolysin o, one well-characterized member of the cytolysin family, is also known to induce specific cd4 and cd8 t cell responses upon infection of mice with listeria monocytogenes. here we describe an hla-drb1*0301-restricted listeriolysin o-derived t cell epitope that is conserved among several members of the cytolysin family. an hla-drb1*030 ... | 2006 | 16798043 |
| outbreak of human listeriosis associated with tomme cheese in northwest switzerland, 2005. | during an eight week period in spring 2005, 10 cases of listeriosis were reported in a small area of northwest switzerland (150,000 inhabitants). eight cases were in older immunocompromised patients who became ill with bacteraemia (three deaths), and two cases were in pregnant women who had septic abortion. all cases were due to a serotype 1/2a isolate with one of two pulsovars found by pfge. patient interviews quickly revealed that a locally made and distributed soft cheese (known as 'tomme') w ... | 2006 | 16801693 |
| listeria outbreak associated with sandwich consumption from a hospital retail shop, united kingdom. | an outbreak of listeriosis occurred in the swindon area of the uk in autumn 2003. five cases were detected in pregnant women. four of these women were thought to have eaten prepacked sandwiches from a retail outlet in one particular hospital. sampling at the supplier detected listeria monocytogenes, which was indistinguishable on molecular testing from the patients isolates. recent changes in uk food legislation should help diminish the risk of further outbreaks/cases such as ours occurring. | 2006 | 16801694 |
| significant increase of listeriosis in germany--epidemiological patterns 2001-2005. | listeriosis has been a mandatorily notifiable disease in germany since january 2001. clinical cases with isolation of listeria monocytogenes from sterile specimens or neonates are reported to the robert koch-institut. listeriosis incidence significantly increased from 0.26 per 100 000 inhabitants (217 cases) in 2001 to 0.62 per 100 000 (519 cases) in 2005. the increase only occurred among non pregnancy-associated cases and was mainly due to a rise in cases in the age group > or =60 years. the hi ... | 2006 | 16801695 |
| surveillance of listeriosis in finland during 1995-2004. | we analysed the surveillance data from listeriosis cases notified to the finnish national infectious diseases register between 1995 and 2004 and describe our recent experience in investigating clusters of listeriosis cases. the number of annual cases varied between 18 and 53 but no trends in incidence were identified (average annual incidence was 7 cases per million inhabitants). only a few cases affected pregnant women or newborns. most of the patients were elderly people with non-malignant und ... | 2006 | 16801696 |
| surveillance of human listeriosis in france, 2001-2003. | mandatory notification of listeriosis began in france in 1999. enhanced public health surveillance, including routine molecular characterisation of listeria monocytogenes strains, epidemiologic follow up of cases, and collection of food samples, has improved the sensitivity of outbreak detection and response. the incidence of listeriosis declined from 4.5 cases/million in 1999-2000 to approximately 3.5 cases/million during the period 2001-2003. clinical, demographic and microbiological character ... | 2006 | 16801697 |
| a multifunctional micro-fluidic system for dielectrophoretic concentration coupled with immuno-capture of low numbers of listeria monocytogenes. | in this study, we demonstrated a micro-fluidic system with multiple functions, including concentration of bacteria using dielectrophoresis (dep) and selective capture using antibody recognition, resulting in a high capture efficiency of bacterial cells. the device consisted of an array of oxide covered interdigitated electrodes on a flat silicon substrate and a approximately 16 microm high and approximately 260 microm wide micro-channel within a pdms cover. for selective capture of listeria mono ... | 2006 | 16804594 |
| fluorescence microscopy of nacl-stressed, elongated salmonella and listeria cells reveals the presence of septa in filaments. | the cell morphology of salmonella enteritidis and listeria monocytogenes after the application of stress was examined. cells were stressed by plating the bacteria on tryptone soya agar (tsa) plates, with 5-10% nacl. the plates were subsequently incubated for 6 days at 25 degrees c. finally, the cells were harvested and subjected to different fluorescent probes in order to visualize the possible presence of septa in elongated cells. use of the stain 4',6-diamidino-2-phenylindole (dapi), which is ... | 2006 | 16806551 |
| first results of the active surveillance of listeria monocytogenes infections in the netherlands reveal higher than expected incidence. | 2006 | 16809829 | |
| structure-based synthetic mimicry of discontinuous protein binding sites: inhibitors of the interaction of mena evh1 domain with proline-rich ligands. | the mena evh1 domain, a protein-interaction module involved in actin-based cell motility, recognizes proline-rich ligand motifs, which are also present in the sequence of the surface protein acta of listeria monocytogenes. the interaction of acta with host mena evh1 enables the bacterium to actively recruit host actin in order to spread into neighboring cells. based on the crystal structure of mena evh1 in complex with a polyproline peptide ligand, we have generated a range of assembled peptides ... | 2006 | 16810654 |
| technological characterization of a bacteriocin-producing lactobacillus sakei and its use in fermented sausages production. | the aim of this paper was the technological characterization of a lactobacillus sakei strain, able to produce the bacteriocin sakacin p, that was originally isolated from naturally fermented sausages. experiments were conducted in situ, using mrs-based medium, and in situ, when the strain was inoculated as starter culture in real sausage fermentation. the results obtained underlined that the strain was able to grow in conditions that are commonly used in the production line, and only lactose and ... | 2006 | 16814893 |
| detection of listeria monocytogenes in food using a combined enrichment/real-time pcr method targeting the prfa gene. | a combined enrichment/real-time pcr method for the detection of listeria monocytogenes is presented. the method is based on a conventional pcr assay targeting the prfa gene, which has been validated and suggested as an international standard pcr method for identifying l. monocytogenes in food. this real-time pcr assay includes an internal amplification control. inclusivity and exclusivity were 100% each when testing 100 l. monocytogenes isolates, 30 listeria spp. isolates other than l. monocytog ... | 2006 | 16814987 |
| pathogenic role for virus-specific cd4 t cells in mice with coronavirus-induced acute encephalitis. | acute viral encephalitis is believed to result from direct virus destruction of infected cells and from virus-induced host immune response, but the relative contribution of each remains largely unknown. for example, c57bl/6 (b6) mice infected with mouse hepatitis virus (jhm strain, jhmv) develop severe encephalitis, with death occurring within 7 days. here, we show that the host response to a single jhmv-specific immunodominant cd4 t-cell epitope is critical for severe disease. we engineered a r ... | 2006 | 16816374 |
| comparison of the concentrations of phenolic compounds in olive oils and other plant oils: correlation with antimicrobial activity. | the antimicrobial activity of different edible vegetable oils was studied. in vitro results revealed that the oils from olive fruits had a strong bactericidal action against a broad spectrum of microorganisms, this effect being higher in general against gram-positive than gram-negative bacteria. thus, olive oils showed bactericidal activity not only against harmful bacteria of the intestinal microbiota (clostridium perfringens and escherichia coli) also against beneficial microorganisms such as ... | 2006 | 16819902 |
| differential expansion, activation and effector functions of conventional and plasmacytoid dendritic cells in mouse tissues transiently infected with listeria monocytogenes. | dendritic cells (dc) are crucial in generating immunity to infection. here we characterize changes in dc in terms of number, activation and effector functions, focusing on conventional dc (cdc) and plasmacytoid dc (pdc), in listeria-infected mice. kinetic studies showed a subset- and tissue-specific expansion of cdc and upregulation of cd80 and cd86 on splenic and mesenteric lymph node (mln) cdc after intragastric infection. expansion of pdc was more prolonged than cdc, and pdc upregulated cd86 ... | 2006 | 16819969 |
| isolation and characterization of listeria monocytogenes isolates from ready-to-eat foods in florida. | of 3,063 ready-to-eat food samples tested, 91 (2.97%) were positive for listeria monocytogenes, and lineage 1 strains outnumbered lineage 2 strains 57 to 34. seventy-one isolates (78%) exhibited multiple antibiotic resistance, and an l. monocytogenes-specific bacteriophage cocktail lysed 65 of 91 (71%) isolates. determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods. | 2006 | 16820508 |
| feature extraction from light-scatter patterns of listeria colonies for identification and classification. | bacterial contamination by listeria monocytogenes not only puts the public at risk, but also is costly for the food-processing industry. traditional biochemical methods for pathogen identification require complicated sample preparation for reliable results. optical scattering technology has been used for identification of bacterial cells in suspension, but with only limited success. therefore, to improve the efficacy of the identification process using our novel imaging approach, we analyze bact ... | 2006 | 16822056 |
| inhibition of membrane bound atpases of escherichia coli and listeria monocytogenes by plant oil aromatics. | previous studies have reported that the mechanism of bactericidal action of the plant oil aromatics, eugenol, carvacrol and cinnamaldehyde involves inhibition of adenosine triphosphate generation and membrane disruption. in this study the capacity of the aromatics to inhibit the membrane bound atpase activity of escherichia coli and listeria monocytogenes was investigated by experiments on isolated membranes. inhibition of the atpase activity of e. coli membranes was observed with 5 mm or 10 mm ... | 2006 | 16828188 |
| phage for rapid detection and control of bacterial pathogens in food. | 2006 | 16829259 | |
| an updated review of listeria monocytogenes in the pork meat industry and its products. | pork meat and processed pork products have been the sources of outbreaks of listeriosis in france and in other european countries during the last decade. the aim of this review is to understand how contamination, survival and growth of listeria monocytogenes can occur in pork meat products. this study discusses the presence of l. monocytogenes in raw pork meat, in the processing environment and in finished products. the prevalence of l. monocytogenes generally increases from the farm to the manu ... | 2006 | 16834586 |
| comparative evaluation of adhesion, surface properties, and surface protein composition of listeria monocytogenes strains after cultivation at constant ph of 5 and 7. | to analyse the cellular mechanisms that influence listeria monocytogenes adhesion onto inert surfaces under acidic growth conditions. | 2006 | 16834591 |
| antimicrobial potential of four lactobacillus strains isolated from breast milk. | the antimicrobial potential of four lactobacilli (lactobacillus salivarius cect5713, lactobacillus gasseri cect5714, l. gasseri cect5715 and lactobacillus fermentum cect5716), isolated from fresh human breast milk, was evaluated in this study and compared with lactobacillus coryniformis cect5711, a reuterin-producing strain isolated from an artisan goat's cheese. | 2006 | 16834593 |
| the cadmium transport sites of cada, the cd2+-atpase from listeria monocytogenes. | cada, the cd(2+)-atpase from listeria monocytogenes, belongs to the zn(2+)/cd(2+)/pb(2+)-atpase bacterial subfamily of p(1b)-atpases that ensure detoxification of the bacteria. whereas it is the major determinant of listeria resistance to cd(2+), cada expressed in saccharomyces cerevisiae severely decreases yeast tolerance to cd(2+) (wu, c. c., bal, n., pérard, j., lowe, j., boscheron, c., mintz, e., and catty, p. (2004) biochem. biophys. res. commun. 324, 1034-1040). this phenotype, which refle ... | 2006 | 16835223 |
| characterization of a bacteriocin-like substance produced by bacillus amyloliquefaciens isolated from the brazilian atlantic forest. | a bacillus strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16s rdna sequencing. phylogenetic analysis indicated that the strain has high sequence similarity with bacillus amyloliquefaciens. the antimicrobial substance was inhibitory to pathogenic and food-spoilage bacteria, such as listeria monocytogenes, bacillus cereus, serratia marcescens, and pasteurella haemolytica. it was stable over a wide temperature range, but lost activity when the temperatu ... | 2006 | 16835841 |
| crystal structure of phosphatidylglycerophosphatase (pgpase), a putative membrane-bound lipid phosphatase, reveals a novel binuclear metal binding site and two "proton wires". | phosphatidylglycerophosphatase (pgpase), an enzyme involved in lipid metabolism, catalyzes formation of phosphatidylglycerol from phosphatidylglycerophosphate. phosphatidylglycerol is a multifunctional phospholipid, found in the biological membranes of many organisms. here, we report the crystal structure of listeria monocytogenes pgpase at 1.8 a resolution. pgpase, an all-helical molecule, forms a homotetramer. each protomer contains an independent active site with two metal ions, ca(2+) and mg ... | 2006 | 16838328 |
| ecology of listeria spp. in a fish farm and molecular typing of listeria monocytogenes from fish farming and processing companies. | this study focused on the ecology of listeria monocytogenes in a fish farm by following the changes in its occurrence in different types of samples for a three year period. in addition, l. monocytogenes isolates from different seafood industry areas were compared with pulsed field gel electrophoresis (pfge) typing to discover possible associations between primary production, further processing and final products. weather conditions were found to have a strong influence on the probability of find ... | 2006 | 16842875 |
| serological and molecular ecology of listeria monocytogenes isolates collected from 13 french pork meat salting-curing plants and their products. | the purpose of this study was dual: 1. to evaluate the serotype distribution of 1028 listeria monocytogenes isolates collected in 13 french salting factories and their products and 2. to identify sources of l. monocytogenes contamination in these factories and trace the routes of spread by pfge (pulsed-field gel electrophoresis) typing. serotypes 1/2a, 1/2b, 1/2c, 4b and 4e occurred. pulsotype diversity was high among strains collected in plants and products. furthermore, strains showing similar ... | 2006 | 16843563 |
| isolation of bacteriocinogenic lactobacillus plantarum strains from ben saalga, a traditional fermented gruel from burkina faso. | a collection of lactic acid bacteria isolated from ben saalga, a traditional fermented gruel from burkina faso, was screened for bacteriocin production. seven isolates were selected for their broad antimicrobial spectra, which overall included strains of bacillus cereus, bacillus licheniformis, enterococcus faecalis, listeria innocua, listeria monocytogenes, staphylococcus aureus, escherichia coli and salmonella enterica. cluster analysis of rapd-pcr patterns revealed that six of the isolates re ... | 2006 | 16844251 |
| ph and solute concentration of suspension media affect the outcome of high hydrostatic pressure treatment of listeria monocytogenes. | the effect of ph and solute concentration of suspension media on high hydrostatic pressure (hhp) induced inactivation of listeria monocytogenes (approximate 10(8) cfu/ml) was investigated by the using treatment between 300 mpa and 600 mpa at 25 degrees c for 10 min. the suspension media used in this study represented different concentrations (0.1% to 10%) of buffered peptone water (bpw) with an adjusted ph of 4 to 7. an increase in the concentration of bpw resulted in a decreased hhp-induced ina ... | 2006 | 16844252 |
| listeria monocytogenes traffics from maternal organs to the placenta and back. | infection with listeria monocytogenes is a significant health problem during pregnancy. this study evaluates the role of trafficking between maternal organs and placenta in a pregnant guinea pig model of listeriosis. after intravenous inoculation of guinea pigs, the initial ratio of bacteria in maternal organs to placenta was 10(3)-10(4):1. rapid increase of bacteria in the placenta changed the ratio to 1:1 after 24 h. utilizing two wild-type strains, differentially marked by their susceptibilit ... | 2006 | 16846254 |
| quantitative assessment of hard surface disinfectant activity against the foodborne pathogen listeria monocytogenes. | listeria monocytogenes is an important foodborne pathogen that must be controlled to ensure food safety. for the years 2003 and 2004, l. monocytogenes caused 20 deaths per 100 listeriosis cases and was responsible for most food recalls for pathogen contamination. the objective of this work was to develop a quantitative method to assess disinfectant activity against l. monocytogenes. standard procedures for testing disinfectants against 3 bacteria are described in the aoac official methods of ana ... | 2006 | 17225610 |
| [expression of acta gene of listeria monocytogenes in escherichia coli and preparation of acta monoclonal antibodies]. | the acta gene was amplified from lm-4 strain of listeria monocytogenes serotype 1/2a by pcr and inserted into t vector. sequencing showed acta gene was 1833bp long and nucleotide homology was 100% compared with acta gene of listeria monocytogenes egd strain in genbank. the cloned acta gene was then inserted into prokaryotic expression vector pgex-6p-1 and pet respectively. the predicted fusion protein was detected by sds-page after iptg induction of recombinant bacteria. the fusion protein expre ... | 2006 | 17302169 |
| the molecular mechanisms that control function and death of effector cd4+ t cells. | this review features two areas of our research interests. first, our laboratory is interested in elucidating the molecular mechanisms that control generation, effector function, and fate of th1 cells during inflammatory responses. we have been particularly interested in the role of the growth arrest and dna damage-inducible protein 45 (gadd45) gene family in th1-mediated immune responses. we and others have shown that, in th1 cells, gadd45b and gadd45g are induced by tcr signaling or il-12 and i ... | 2006 | 17337788 |
| brain abscess due to listeria monocytogenes: first case report in thailand. | brain abscess with bacteremia caused by listeria monocytogenes in a young woman with immune thrombocytopenic purpura was reported. the clinical features included fever, headache, and left-side weakness. computed tomography and magnetic resonance imaging of the brain showed a large single abscess at the right frontoparietal area. l. monocytogenes was isolated from a blood culture. the patient promptly received a surgical drainage. because she had a history of penicillin allergy, and the organism ... | 2006 | 17100394 |
| indoleamine 2,3-dioxygenase-expressing dendritic cells form suppurative granulomas following listeria monocytogenes infection. | control of pathogens by formation of abscesses and granulomas is a major strategy of the innate immune system, especially when effector mechanisms of adaptive immunity are insufficient. we show in human listeriosis that dcs expressing indoleamine 2,3-dioxygenase (ido), together with macrophages, are major cellular components of suppurative granulomas in vivo. induction of ido by dcs is a cell-autonomous response to listeria monocytogenes infection and was also observed in other granulomatous inf ... | 2006 | 17111046 |
| cutting edge: il-12 inversely regulates t-bet and eomesodermin expression during pathogen-induced cd8+ t cell differentiation. | cytokines are critical determinants for specification of lineage-defining transcription factors of cd4+ t cell subsets. little is known, however, about how cytokines regulate expression of t-bet and eomesodermin (eomes) in effector and memory cd8+ t cells. we now report that il-12, a signature of cell-mediated immunity, represses eomes while positively regulating t-bet in effector cd8+ t cells during infection with listeria monocytogenes. after resolution of infection and abatement of il-12 sign ... | 2006 | 17114419 |
| ifn-beta-mediated up-regulation of cd1d in bacteria-infected apcs. | the expression of cd1d molecules is essential for the selection and activation of a unique subset of t cells, invariant nkt cells, which express limited tcr diversity and have been demonstrated to function in both regulatory and antimicrobial immune responses. although it has been reported that the levels of cd1d expression can be modulated during infection, the mechanisms that mediate this effect are poorly defined. in this study, we show that infection of dendritic cells and macrophages both i ... | 2006 | 17114455 |
| microflora of minimally processed frozen vegetables sold in gaborone, botswana. | two hundred samples of minimally processed, frozen, and prepacked potato chips, peas, corn, and a variety of combined vegetables from supermarkets in gaborone, botswana, were examined microbiologically. determination of aerobic mesophilic plate count, aerobic psychrotrophic plate count, lactic acid bacteria, yeasts and molds, coliforms, listeria spp., and staphylococcus aureus were done. chips had the lowest mean log values for all of the microorganisms enumerated except yeasts and molds. the me ... | 2006 | 17133799 |
| growth and stress resistance variation in culture broth among listeria monocytogenes strains of various serotypes and origins. | twenty-five listeria monocytogenes strains of various serotypes and sources, including clinical and food isolates associated with the same outbreaks, were characterized and compared based on growth rates and heat and acid death rates. growth was monitored in tryptic soy broth supplemented with 0.6% yeast extract (tsbye) at 4 and 30 degrees c for 32 days and 20 h, respectively. heat and acid stress responses in tsbye heated to 55 degrees c or acidified to ph 3.0 with lactic acid were evaluated fo ... | 2006 | 17133807 |
| quantifying nonthermal inactivation of listeria monocytogenes in european fermented sausages using bacteriocinogenic lactic acid bacteria or their bacteriocins: a case study for risk assessment. | listeria monocytogenes nctc10527 was examined with respect to its nonthermal inactivation kinetics in fermented sausages from four european countries: serbia-montenegro, hungary, croatia, and bosnia-herzegovina. the goal was to quantify the effect of fermentation and ripening conditions on l. monocytogenes with the simultaneous presence or absence of bacteriocin-producing lactic acid bacteria (i.e., lactobacillus sakei). different models were used to fit the experimental data and to calculate th ... | 2006 | 17133808 |
| intragastric inoculation with a cocktail of listeria monocytogenes strains does not potentiate the severity of infection in a/j mice compared to inoculation with the individual strains comprising the cocktail. | although multistrain cocktails of listeria monocytogenes are used in food inoculation experiments, no studies, to our knowledge, have been reported that use these cocktails in an intragastric mouse model. in this study, we used a five-strain l. monocytogenes cocktail consisting of strains scott a, mfs108, 101m, v7, and 310 and a four-strain l. monocytogenes cocktail containing strains scott a, egd, h7738, and f2365. here, we report that intragastric inoculation of anesthetized mice with approxim ... | 2006 | 17133809 |
| effects of microbial inhibitors and modified atmosphere packaging on growth of listeria monocytogenes and salmonella enterica typhimurium and on quality attributes of injected pork chops and sliced cured ham. | this study was designed to determine the inhibitory effects of modified atmosphere packaging (map), both alone and in combination with potassium lactate and sodium diacetate (plsd), on selected pathogens common to pork products. effects of the treatments on product quality also were assessed. the hypothesis was that high-co2 map would increase the effectiveness of plsd for inhibition of pork pathogens. fresh chops from untreated pork loins and loins that were injected with plsd were inoculated w ... | 2006 | 17133810 |
| assessment of environmental factors on listeria monocytogenes scott a inla gene expression by relative quantitative taqman real-time reverse transcriptase pcr. | several virulence factors are involved in listeria monocytogenes pathogenicity. l. monocytogenes internalins, particularly internalin a, are required for bacterial adhesion to and invasion of human intestinal epithelial cells. the expression of internalins is thus related to virulence. identification of conditions involved in regulating the expression of l. monocytogenes virulence factors is essential for developing targeted strategies to control listeriosis incidence and improving therapeutic a ... | 2006 | 17133822 |
| inactivation of the crp/fnr family of regulatory genes in listeria monocytogenes strain f2365 does not alter its heat resistance at 60 degrees c. | a surprising facet of the listeria monocytogenes genome is the presence of 15 genes that code for regulators in the crp/ fnr family and include the virulence regulator prfa. the genes under the transcriptional control of these regulators are currently undetermined, with the exception of some genes controlled by the major virulence regulator prfa. using 12 strains of l. monocytogenes, each with an inserted gene cassette that interrupts and renders nonfunctional a different l. monocytogenes strain ... | 2006 | 17133823 |
| interval mapping of quantitative trait loci for time-to-event data with the proportional hazards mixture cure model. | interval mapping using normal mixture models has been an important tool for analyzing quantitative traits in experimental organisms. when the primary phenotype is time-to-event, it is natural to use survival models such as cox's proportional hazards model instead of normal mixtures to model the phenotype distribution. an extra challenge for modeling time-to-event data is that the underlying population may consist of susceptible and nonsusceptible subjects. in this article, we propose a semiparam ... | 2006 | 17156279 |
| a bifunctional o-glcnac transferase governs flagellar motility through anti-repression. | flagellar motility is an essential mechanism by which bacteria adapt to and survive in diverse environments. although flagella confer an advantage to many bacterial pathogens for colonization during infection, bacterial flagellins also stimulate host innate immune responses. consequently, many bacterial pathogens down-regulate flagella production following initial infection. listeria monocytogenes is a facultative intracellular pathogen that represses transcription of flagellar motility genes at ... | 2006 | 17158746 |
| listeria monocytogenes in a young patient with non hodgkins lymphoma: case report. | listeria monocytogenes is an intracellular food-borne pathogen, widely distributed in the environment, which rarely causes clinical infection in healthy people, but may cause severe disease in immunocompromised patients. a case of listeriosis is certified in an immunocompromised patient, thus confirming this microorganism to be an opportunistic human pathogen. | 2006 | 17166414 |
| [spontaneous bacterial peritonitis caused by listeria in a patient with cirrhosis: case report]. | infections caused by listeria monocytogenes are usually found in cattle and occasionally appear in humans, particularly pregnant women and immunocompromised individuals. peritonitis by listeria monocytogenes is a rare but dangerous condition that must be recognized early, since it requires a specific treatment. we report a 31 year-old male with alcoholic cirrhosis that developed ascites with abdominal pain and fever. the peritoneal fluid culture yielded listeria monocytogenes. the patients was i ... | 2006 | 17171220 |
| [molecular typing of listeria monocytogenes isolated from clinical and food samples]. | listeria monocytogenes is an emergent foodborne pathogen acquired by the ingestion of contaminated food. this bacterium causes a disease called listeriosis, whose mortality rate world wide is around 20% to 30%, reaching up to 80% in cases of neonatal infections. the random amplified polymorphic dna technique allows different isolates to be distinguished and characterized at the molecular level, which can provide useful information about the diversity of this pathogen in colombia. | 2006 | 17176008 |
| microbiological baseline study of broiler chickens at swedish slaughterhouses. | this 1-year study was conducted to estimate the prevalence and concentrations of pathogenic and indicator bacteria on swedish broiler chickens. a total of 636 chilled carcasses were collected from 10 slaughterhouses and sent to the national food administration for analyses of carcass rinses. no carcasses were positive for salmonella. campylobacter, predominantly campylobacter jejuni, were detected on 15% (by enrichment) or 14% (by direct plating) of the carcasses. with one exception, all samples ... | 2006 | 17186653 |
| international life sciences institute north america listeria monocytogenes strain collection: development of standard listeria monocytogenes strain sets for research and validation studies. | research and development efforts on bacterial foodborne pathogens, including the development of novel detection and subtyping methods, as well as validation studies for intervention strategies can greatly be enhanced through the availability and use of standardized strain collections. these types of strain collections are available for some foodborne pathogens, such as salmonella and escherichia coli. we have developed a standard listeria monocytogenes strain collection that has not been previou ... | 2006 | 17186661 |
| transfer of listeria monocytogenes during slicing of turkey breast, bologna, and salami with simulated kitchen knives. | in response to continued concerns regarding listeria cross-contamination during the slicing of deli meats, a series of specially prepared grade 304 and 316 stainless steel kitchen knife blades was inoculated with a six-strain listeria monocytogenes cocktail (10(8), 10(5), and 10(3) cfu per blade) composed of two weak, two medium, and two strong biofilm-forming strains. the blades were then attached to an instron 5565 electromechanical compression analyzer and used to slice whole chubs of delicat ... | 2006 | 17186662 |
| inhibition and inactivation of listeria monocytogenes and escherichia coli o157:h7 colony biofilms by micellar-encapsulated eugenol and carvacrol. | the antimicrobial efficacy of carvacrol and eugenol, two essential oil compounds, encapsulated in a micellar nonionic surfactant solution on four strains of listeria monocytogenes (scott a, 101, 108, and 310) and four strains of escherichia coli o157:h7 (h1730, e0019, f4546, and 932) growing as colony biofilms was investigated. carvacrol and eugenol were encapsulated in surfynol 485w at concentrations ranging from 0.3 to 0.9% (wt/wt) at a surfactant concentration of 5% (wt/wt). colony biofilms w ... | 2006 | 17186663 |
| irradiation inactivation of listeria monocytogenes in low-fat ground pork at freezing and refrigeration temperatures. | gamma radiation effectively controls listeria monocytogenes in uncooked and in ready-to-eat foods. this study was conducted to determine if gamma radiation could be used to control l. monocytogenes in ground pork. ground pork was contaminated with l. monocytogenes, kept at refrigeration (4 degrees c), chilling (0 degrees c), and freezing (-18 degrees c) temperatures overnight, exposed to gamma radiation and stored at 4 degrees c for 7 days, and at 0 and -18 degrees c for 60 days. following irrad ... | 2006 | 17186664 |
| effect of gamma radiation and oregano essential oil on murein and atp concentration of listeria monocytogenes. | the effects of gamma radiation and of oregano essential oil alone or in combination with radiation on murein composition of listeria monocytogenes and on the intracellular and extracellular concentration of atp were evaluated. the bacterial strain was treated with two radiation doses, 1.2 kgy to induce cell damage and 3.5 kgy to cause cell death. oregano essential oil was used at 0.020 and 0.025% (wt/vol), which is the mic. all treatments had a significant effect (p < or = 0.05) on the murein co ... | 2006 | 17186665 |
| heat inactivation of listeria monocytogenes and salmonella enterica serovar typhi in a typical bologna matrix during an industrial cooking-cooling cycle. | the heat resistance of salmonella enterica serovar typhi pf-724 and listeria monocytogenes 2812 was determined in a commercial bologna batter. the heat inactivation of the two bacterial species was also studied in a semiautomatic pilot smokehouse under cooking conditions that reproduced an industrial bologna process. s. enterica serovar typhi pf-724 was less heat resistant than l. monocytogenes 2812. the d-values (times required to reduce the population by 1 logarithmic cycle) for s. enterica se ... | 2006 | 17186675 |
| role of uvra in the growth and survival of listeria monocytogenes under uv radiation and acid and bile stress. | listeria monocytogenes encounters numerous stresses both in the food environment and during infection of the host. the ability to survive and tolerate bile and low ph conditions, which are two major stresses, is of particular importance for survival within the host. the uvra gene in other bacteria is involved in the repair of acid-induced dna damage and in adaptation to low ph. thus, a uvra in-frame deletion mutant was constructed to identify the role of uvra in the growth and survival of l. mon ... | 2006 | 17186676 |
| survival and growth of foodborne pathogens during cooking and storage of oriental-style rice cakes. | fresh cooked rice cakes for retail sale are typically held at room temperature because refrigeration dramatically reduces their quality. room temperature, high water activity, and a ph of > 4.6 provided an environment conducive to pathogen growth. to date, no studies have been published regarding survival and growth of foodborne pathogens in fresh cooked rice cakes. this study was undertaken to investigate the effect of steam cooking on foodborne pathogens and their subsequent growth in five var ... | 2006 | 17186677 |
| listeria monocytogenes in multiple habitats and host populations: review of available data for mathematical modeling. | listeria monocytogenes has the ability to survive and multiply in diverse habitats and to cause infection in a variety of animal species and humans. we evaluated the literature on survival and multiplication within and transmission among multiple host populations and habitats, including man, sewage, general environment (soil, water, and vegetation), silage (fermented plant material), animals (including wild and domestic animals), and food processing plants. the available knowledge on l. monocyto ... | 2006 | 17199514 |
| evaluation of effects of primary and secondary enrichment for the detection of listeria monocytogenes by real-time pcr in retail ground chicken meat. | a sybr green i based real-time pcr assay with inla-specific oligonucleotide primers was developed for easy and rapid detection of listeria monocytogenes in a model food that usually has a high incidence of contamination with this pathogen. results with pure cultures and artificially contaminated chicken meat samples indicate that the pcr assay was highly specific and sensitive. the melting point analysis of the 160 bp amplified dna fragment was different for l. monocytogenes isolates of the two ... | 2006 | 17199516 |
| characterization of an hmg-coa reductase from listeria monocytogenes that exhibits dual coenzyme specificity. | hmg-coa reductase (hmgr) is an enzyme critical for cellular cholesterol synthesis in mammals and isoprenoid synthesis in certain eubacteria, catalyzing the nad(p)h-dependent reduction of hmg-coa to mevalonate. we have isolated the gene encoding hmg-coa reductase from listeria monocytogenes and expressed the recombinant 6x-his-tagged form in escherichia coli. using nad(p)(h), the enzyme catalyzes hmg-coa reduction approximately 200-fold more efficiently than mevalonate oxidation in vitro. the pur ... | 2006 | 17128979 |
| listeria monocytogenes meningitis and multiple brain abscesses in an immunocompetent host. favorable response to combination linezolid-meropenem treatment. | 2006 | 17129848 | |
| effect of co2 on the resuscitation of listeria monocytogenes injured by mild inactivation techniques. | 2006 | 17191465 | |
| microbial safety of mayonnaise based salads: a growth/no growth model for listeria monocytogenes as a predictive tool. | 2006 | 17191531 | |
| [a fatal case of listeria monocytogenes sepsis and meningitis in a patient with wegener's granulomatosis]. | the bacillus listeria monocytogenes is widely distributed in the environment. listeria monocytogenes most often causes infection in the neonates, pregnant women, elderly and immunosuppressed persons. we report on a case of fatal sepsis and meningitis in a 59-year-old woman receiving cyclophosphamide and glucocorticoid therapy for wegener's granulomatosis over a 10-year period. listeriosis should be suspected in case of sepsis and/or meningitis in patients who receive immunosuppressive agents. si ... | 2006 | 17217109 |
| dimeric structure of the cell shape protein mrec and its functional implications. | the bacterial actin homologue mreb forms helical filaments in the cytoplasm of rod-shaped bacteria where it helps maintain the shape of the cell. mreb is co-transcribed with mrec that encodes a bitopic membrane protein with a major periplasmic domain. like mreb, mrec is localized in a helical pattern and might be involved in the spatial organization of the peptidoglycan synthesis machinery. here, we present the structure of the major, periplasmic part of mrec from listeria monocytogenes at 2.5 a ... | 2006 | 17427287 |
| "mini-array" transcriptional analysis of the listeria monocytogenes lecithinase operon as a class project: a student investigative molecular biology laboratory experience*. | this report describes a molecular biotechnology-based laboratory curriculum developed to accompany an undergraduate genetics course. during the course of a semester, students researched the pathogen, developed a research question, designed experiments, and performed transcriptional analysis of a set of genes that confer virulence to the food-borne pathogen, listeria monocytogenes. gene fragments were amplified via pcr and utilized in "mini-arrays," a dot-blot-based format suitable for the simult ... | 2006 | 21638678 |
| role of quantitative risk assessment and food safety objectives in managing listeria monocytogenes on ready-to-eat meats. | listeria monocytogenes may be found on ready-to-eat (rte) meats, posing a public health risk. to minimize the public health impact, an appropriate level of protection (alop) can be established for a population with respect to l. monocytogenes, and ideally should be based on a scientific assessment of the risk, as well as societal and economic factors. food safety systems can be based on meeting the alop. food safety objectives (fso) provide a link between the alop and performance objectives that ... | 2006 | 22062717 |
| prevalence of listeria species in camel sausages from retail markets in aydin province in turkey and rapd analysis of listeria monocytogenes isolates. | samples were taken from 100 camel sausages from the different retail markets in aydin province in the south-west of turkey and they were tested for the presence of listeria spp by biochemical methods. samples were enriched using listeria enrichment broth and they were inoculated onto listeria selective agar. listeria monocytogenes was isolated from nine samples (9%), listeria innocua from 14 samples (14%) and listeria welshimeri from two samples(2%). a 701 bp fragment of listeriolysin o sequence ... | 2006 | 21851683 |
| a note on the incidences of salmonella spp., listeria spp. and escherichia coli o157:h7 serotypes in turkish sausage (soudjouck). | the incidence of salmonella spp., listeria monocytogenes and escherichia coli o157:h7 was determined in 100 turkish sausage (soudjouck) samples collected from shops and markets in the afyon province, turkey. salmonella spp. were detected in 7% of the samples. all of the isolates were s. enterica paratyphi b. in addition, listeria spp. were detected in 9% of the samples. its distribution was 7% l. monocytogenes and 1% each of l. ivanovii and l. innocua. serological study of the seven l. monocytog ... | 2006 | 22061388 |
| effect of a bacteriocin produced by pediococcus acidilactici against listeria monocytogenes and clostridium perfringens on spanish raw meat. | the inhibitory effect of a bacteriocin, produced by pediococcus acidilactici, against listeria monocytogenes and clostridium perfringens on spanish raw meat surface, was evaluated by in situ assays. samples were incubated with the bacteriocin and then with a culture of the pathogenic bacteria. the treatment with 500, 1000 or 5000 bacteriocin units/ml (bu/ml) reduced the counts of l. monocytogenes after storage at 15°c during 72h by 1, 2 or 3 log cycles and with 1000 or 5000 bu/ml after storage a ... | 2006 | 22061374 |
| antilisterial activity of lactic acid bacteria inoculated on cooked ham. | this study was conducted to evaluate the ability of lactobacillus sakei 1, a bacteriocin-producing (bac(+)) lactic acid bacterium (lab), isolated from brazilian fresh pork sausage to inhibit two listeria monocytogenes strains (serotypes 4b and 1/2a) on cooked, sliced vacuum-packaged ham. l. sakei atcc 15521 was used as a non-bacteriocin producer (bac(-)). l. monocytogenes (ca. 2 logcfu/ml) and lab (ca. 6 logcfu/ml) were inoculated on the sterilized ham, vacuum-sealed and incubated at 8°c for 10 ... | 2006 | 22063215 |
| microbial heat resistance of listeria monocytogenes and the impact on ready-to-eat meat quality after post-package pasteurization. | several methods using bactericides, hydrostatic pressure, and post-package pasteurization technologies to control listeria monocytogenes (lm) in ready-to-eat meats have been attempted. in addition to controlling lm contamination, any newly developed technology must have minimal effects on organoleptic properties. the objectives of this study were to: (1) determine the heat resistance of lm in two brands (a and b) of bologna differing in formulations, and, (2) evaluate the effects of post-package ... | 2006 | 22063046 |
| the sensory acceptability of cooked meat products treated with a protective culture depends on glucose content and buffering capacity: a case study with lactobacillus sakei 10a. | biopreservation has been proven to be a promising natural preservation technique, but the impact of protective cultures on the sensory properties of cooked meat products (cmp) is not well documented. this work presents a case study on the protective culture lactobacillus sakei 10a to obtain a clear view on the real consequences of using protective cultures on the sensory quality of cmp. a preliminary screening study on 13 different cmp and more elaborate application trials at 7°c on vacuum packa ... | 2006 | 22063058 |