Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a survey of foodborne pathogens in bulk tank milk and raw milk consumption among farm families in pennsylvania. | a 2-part study was conducted to determine the risk of exposure to human pathogens from raw milk. the first part of the study focused on determining raw milk consumption habits of dairy producers. a total of 248 dairy producers from 16 counties in pennsylvania were surveyed. overall, 105 (42.3%) of the 248 dairy producers consumed raw milk and 170 (68.5%) of the 248 dairy producers were aware of foodborne pathogens in raw milk. dairy producers who were not aware of foodborne pathogens in raw milk ... | 2006 | 16772561 |
| invasive listeria monocytogenes infections in the netherlands, 1995-2003. | in order to add to the limited data available about the incidence of invasive listeria monocytogenes infection in the netherlands, two studies were conducted. in the first study, data on hospital patients with listeriosis in the period 1995-2003 were obtained from the national medical registration (study 1). in the second study, hospital discharge letters for patients whose listeria isolates were received by the netherlands reference laboratory for bacterial meningitis (nrlbm) in the period 1999 ... | 2006 | 16773392 |
| listeriolysin o: a key protein of listeria monocytogenes with multiple functions. | cholesterol-dependent cytolysins (cdcs) are produced by a large number of pathogenic gram-positive bacteria. most of these single-chain proteins are secreted in the extracellular medium. among the species producing cdcs, only two species belonging to the genus listeria (listeria monocytogenes and listeria ivanovii) are able to multiply intracellularly and release their toxins in the phagosomal compartment of the infected host cell. this review provides an updated overview on the importance of li ... | 2006 | 16774585 |
| purification and characterization of a heat-stable serine protease inhibitor from the tubers of new potato variety "golden valley". | potide-g, a small (5578.9 da) antimicrobial peptide, was isolated from potato tubers (solanum tuberosum l. cv. golden valley) through extraction of the water-soluble fraction, dialysis, ultrafiltration and deae-cellulose and c18 reverse-phase high performance liquid chromatography. this antimicrobial peptide was heat-stable and almost completely suppressed the proteolytic activity of trypsin, chymotrypsin and papain, with no hemolytic activity. in addition, potide-g potently inhibited growth of ... | 2006 | 16777063 |
| biofilm formation in an ice cream plant. | the sites of biofilm formation in an ice cream plant were investigated by sampling both the production line and the environment. experiments were carried out twice within a 20-day period. first, stainless steel coupons were fixed to surfaces adjacent to food contact surfaces, the floor drains and the doormat. they were taken for the analysis of biofilm at three different production stages. then, biofilm forming bacteria were enumerated and also presence of listeria monocytogenes was monitored. b ... | 2006 | 16779628 |
| characterization of a new bacteriocin produced from a novel isolated strain of bacillus lentus ng121. | the new bacteriocin is produced from bacillus lentus ng121 isolated from khameera - a traditional fermented food from himachal pradesh, india which has been reported for the first time in the literature to produce bacteriocin and exhibited very high activity units of 20 x 10(5) au (arbitrary units)/ml. this bacteriocin was partially purified and was further characterized to assess its preservation characteristics. it showed strong antimicrobial activity against the most challenging and serious t ... | 2006 | 16779629 |
| [ruptured mesenteric aneurysm from listeria monocytogenes]. | infections due to listeria monocytogenes usually occur in pregnant women, in elderly or immunocompromised patients. we report the case of an immunosuppressed woman who had been hospitalized for a meningoencephalitis due to l. monocytogenes. she was operated on for an abdominal hemorrhagic shock due to a ruptured mycotic aneurysm of the inferior mesenteric artery. arterial aneurysms due to this germ are rare and occur on large arteries. only 17 cases have been previously described in the literatu ... | 2006 | 16780785 |
| adsorption on stainless steel surfaces of biosurfactants produced by gram-negative and gram-positive bacteria: consequence on the bioadhesive behavior of listeria monocytogenes. | the ability of adsorbed biosurfactants (pf and lb) obtained from gram-negative bacterium (pseudomonas fluorescens) or gram-positive bacterium (lactobacillus helveticus) to inhibit adhesion of four listerial strains to stainless steel was investigated. these metallic surfaces were characterized using the following complementary analytical techniques: contact-angle measurements (cam), atomic force microscopy (afm), polarization modulation-infrared reflection-adsorption spectroscopy (pm-irras) and ... | 2006 | 16781848 |
| ultrasound markers of fetal infection, part 2: bacterial, parasitic, and fungal infections. | up to 1% of all pregnancies have clinically overt intra-amniotic bacterial infections, and an even larger percentage of pregnant women may be affected by silent infections. although most pregnant women with overt intra-amniotic bacterial infection have experienced prolonged rupture of membranes (prom), symptomatic and most silent nonviral intra-amniotic infections may occur with intact membranes. the etiology of intra-amniotic infection after prom is almost always polymicrobial and consists of g ... | 2006 | 16783243 |
| acute symptomatic hydrocephalus in listeria monocytogenes meningitis. | listeria monocytogenes meningitis appears to have increased in incidence. although most reported cases of listeriosis involve the central nervous system, brain computed tomography is usually normal. hydrocephalus is a common complication of tuberculous meningitis, which has a high prevalence in taiwan. however, patients with l. monocytogenes meningitis rarely develop the complication of symptomatic hydrocephalus. we report a patient with l. monocytogenes meningitis who presented with persistent ... | 2006 | 16783458 |
| evidence for the opposing roles of different gamma delta t cell subsets in macrophage homeostasis. | to ensure invading pathogens are eliminated with minimal damage to host tissues it is essential that macrophage activation be tightly regulated. previously we demonstrated that a subset of gammadelta t cells (vgamma1(+)) contributes to resolving pathogen-induced immune responses by killing activated macrophages. however, the exaggerated macrophage response seen in infected vgamma1(+) t cell-deficient mice suggests that gammadelta t cells play a broader role in macrophage homeostasis and other su ... | 2006 | 16783854 |
| delayed expansion and contraction of cd8+ t cell response during infection with virulent salmonella typhimurium. | ag presentation to cd8(+) t cells often commences immediately after infection, which facilitates their rapid expansion and control of infection. subsequently, the primed cells undergo rapid contraction. we report that this paradigm is not followed during infection with virulent salmonella enterica, serovar typhimurium (st), an intracellular bacterium that replicates within phagosomes of infected cells. although susceptible mice die rapidly (approximately 7 days), resistant mice (129 x 1svj) harb ... | 2006 | 16849458 |
| visualization of il-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote th1 differentiation. | il-12p40 is induced in macrophages and dendritic cells (dc) after activation by microbial tlr ligands and cytokines and constitutes a component of il-12 and il-23. in an effort to understand the location and kinetics of these cytokines during the course of an immune response, we generated knockin (gene-targeted) mice that express the p40 gene linked via a viral internal ribosome entry site element with fluorescent reporters, eyfp or egfp. macrophages and dc from these mice faithfully reported bi ... | 2006 | 16849470 |
| innate inflammatory signals induced by various pathogens differentially dictate the ifn-i dependence of cd8 t cells for clonal expansion and memory formation. | type-i ifns (ifn-i) provide direct survival signals to t cells during ag-driven proliferation. because ifn-i production differs depending on the pathogen, we assessed cd8 t cell requirement for direct ifn-i signals during responses to vaccinia virus (vv), vesicular stomatitis virus (vsv), lymphocytic choriomeningitis virus (lcmv), and listeria monocytogenes (lm) immunizations in vivo. ifn-i-receptor-deficient (ifn-ir(o)) cd8 t cells expanded 3- to 5-fold less and formed a diminished memory pool ... | 2006 | 16849484 |
| the adenosine system selectively inhibits tlr-mediated tnf-alpha production in the human newborn. | human newborns are susceptible to microbial infection and mount poor vaccine responses, yet the mechanisms underlying their susceptibility are incompletely defined. we have previously reported that despite normal basal expression of tlrs and associated signaling intermediates, human neonatal cord blood monocytes demonstrate severe impairment in tnf-alpha production in response to triacylated (tlr 2/1) and diacylated (tlr 2/6) bacterial lipopeptides (blps). we now demonstrate that in marked contr ... | 2006 | 16849509 |
| modelling the individual cell lag time distributions of listeria monocytogenes as a function of the physiological state and the growth conditions. | the individual cell lag time distributions of listeria monocytogenes were characterized for 54 combinations of 22 initial physiological states, 18 growth conditions, and 11 strains. the individual cell lag times were deduced from the times for cultures issued from individual cells to reach an optical density threshold. the extreme value type ii distribution with a shape parameter set to 5 was shown effective to describe the 54 observed distributions. the theoretical distributions of individual l ... | 2006 | 16857284 |
| enhancement of mice susceptibility to infection with listeria monocytogenes by the treatment of morphine. | the effect of morphine on the susceptibility of balb/c mice to diarrheagenic escherichia coli, shigella flexneri, listeria monocytogenes, salmonella enteritidis, yersinia enterocolitica, was examined via the intraperitoneal inoculation. morphine treatment increased the susceptibility to s. enteritidis and l. monocytogenes, resulting in bacteremia and central nervous system (cns) invasion (for l. monocytogenes), while the infection with other bacteria did not show the systemic dissemination in th ... | 2006 | 16858145 |
| regulated translation of listeriolysin o controls virulence of listeria monocytogenes. | listeria monocytogenes is an intracytosolic bacterial pathogen that escapes from the phagosome using a secreted cytolysin, listeriolysin o (llo). in the host cytosol, llo activity is minimized to prevent pore formation in the host plasma membrane; premature lysis of the infected host cell exposes the bacteria to extracellular immune defences of the host and is detrimental to infection. here we identified nucleotide substitutions in the coding sequence of the llo gene (hly) that did not alter the ... | 2006 | 16859495 |
| vaccination with irradiated listeria induces protective t cell immunity. | we evaluated gamma-irradiated listeria monocytogenes as a killed bacterial vaccine, testing the hypothesis that irradiation preserves antigenic and adjuvant structures destroyed by traditional heat or chemical inactivation. irradiated listeria monocytogenes (lm), unlike heat-killed lm, efficiently activated dendritic cells via toll-like receptors and induced protective t cell responses in mice. like live lm, irradiated lm induced toll-like-receptor-independent t cell priming. cross-presentation ... | 2006 | 16860763 |
| isolation of listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction. | listeria monocytogenes, a gram-positive, facultative intracellular pathogen was isolated from buffaloes with a history of reproductive disorders and polymerase chain reaction (pcr) analyses for the presence of virulence-associated genes were conducted. a total of 530 samples of faecal, nasal, vaginal swabs and blood samples from 135 buffaloes were screened. the prevalence of l. monocytogenes and other listeria spp. was found to be 4.4 and 7.4%, respectively. all isolates were subjected to pcr fo ... | 2006 | 16860946 |
| the role of macrophage migration inhibitory factor in lethal listeria monocytogenes infection in mice. | macrophage migration inhibitory factor (mif) has been characterized as a proinflammatory cytokine. previous studies have indicated that mif may play a beneficial role or a detrimental role in microbial infections, depending on pathogens. in this study, we investigated the role of mif in listeria monocytogenes infection. the mif titers increased 6h after lethal l. monocytogenes infection but not in the sublethal infection. the elimination of bacteria from the spleens and livers was not affected b ... | 2006 | 16863687 |
| prevalence of listeria monocytogenes and other listeria species in butter from united kingdom production, retail, and catering premises. | two recent listeriosis outbreaks involving butter prompted this first cross-sectional study on the prevalence, levels, and types of listeria species in 3229 samples of butter from production, retail, and catering premises in the united kingdom during may and june 2004. when the criteria of the microbiological guidelines were used, 99.4% of samples were found to be of satisfactory microbiological quality, 0.5% were of acceptable quality, and 0.1% were of unsatisfactory quality as a result of high ... | 2006 | 16865880 |
| attachment of listeria monocytogenes to an austenitic stainless steel after welding and accelerated corrosion treatments. | austenitic stainless steels, widely used in food processing, undergo microstructural changes during welding, resulting in three distinctive zones: weld metal, heat-affected zone, and base metal. this research was conducted to determine the attachment of listeria monocytogenes in these three zones before and after exposure to a corrosive environment. all experiments were done with tungsten inert gas welding of type 304 stainless steel. the four welding treatments were large or small beads with hi ... | 2006 | 16865881 |
| survival of listeria monocytogenes in uncooked italian dry sausage (salami). | this study was undertaken to supplement existing information on the survival of listeria monocytogenes in italian salami. the fact that italian salami is frequently consumed by a large number of people poses some serious health implications. some raw materials have been found to be microbiologically contaminated, for their production occurs without any thermic treatment, and these are in circulation throughout italy all year round. we selected the product for its microbiological, technological, ... | 2006 | 16865882 |
| a predictive model to describe the effects of temperature, sodium lactate, and sodium diacetate on the inactivation of a serotype 4b strain of listeria monocytogenes in a frankfurter slurry. | a modified gompertz equation was used to model the effects of temperature (55, 60, and 65 degrees c), sodium lactate (0, 2.4, and 4.8%), and sodium diacetate (0, 0.125, and 0.25%) on inactivation of listeria monocytogenes strain mfs 102 (serotype 4b) in frankfurter slurry. the effects of these factors were determined on the shouldering region (parameter a), maximum death rate (parameter b), and tailing region (parameter c) of microbial inactivation curves. increased temperature or sodium diaceta ... | 2006 | 16865885 |
| effect of x-ray irradiation on reducing the risk of listeriosis in ready-to-eat vacuum-packaged smoked mullet. | listeria monocytogenes can pose a serious threat in several areas of the nation's food supply including ready-to-eat seafood products. use of irradiation processing can potentially reduce the risk of listeriosis caused by consumption of ready-to-eat seafood products. this study measured the effect of x-ray irradiation on reducing the population of l. monocytogenes on ready-to-eat, vacuum-packaged smoked mullet. smoked mullet were inoculated with a five-strain mixture of l. monocytogenes (10(4) c ... | 2006 | 16865886 |
| efficacy of disinfectants to reduce listeria monocytogenes on precut iceberg lettuce. | the efficacy of water, chlorinated water (100 ppm), peracetic acid solution (0.05%), and commercial citric acid-based produce wash (0.25%) to reduce the population of listeria monocytogenes on precut lettuce was tested. samples were inoculated with a mixture of equal amounts of five l. monocytogenes strains at a level of 4.7 log cfu/g, and analyzed on the day of washing and after 3 and 6 days of storage at 6 degrees c. sanitizer reduced the number of l. monocytogenes at maximum 1.7 log cfu/g and ... | 2006 | 16865887 |
| adhesion properties and competitive pathogen exclusion ability of bifidobacteria with acquired acid resistance. | the adhesion properties of bifidobacterium longum and bifidobacterium catenulatum strains with an acquired resistance to acid and their ability to competitively exclude salmonella enterica serovar typhimurium, escherichia coli, listeria monocytogenes, enterobacter sakazakii, and clostridium difficile from adhering to human intestinal mucus were evaluated and compared with the results when the same experiments were run with the original acid-sensitive strains. in half of the four studied cases, t ... | 2006 | 16865903 |
| molecular epidemiology and cluster analysis of human listeriosis cases in three u.s. states. | to better understand the transmission and epidemiology of human listeriosis, 647 listeria monocytogenes isolates obtained from human listeriosis cases in four u.s. locations (michigan, ohio, new york state, and new york city) over 61 months (1998 to 2003) were characterized by automated ecori ribotyping. a total of 65 ribotypes were differentiated among the characterized isolates; 393, 227, and 24 isolates were classified into lineages i, ii, and iii, respectively, and 3 isolates were not classi ... | 2006 | 16865904 |
| antimicrobial activity of extracts of the lichen xanthoparmelia pokornyi and its gyrophoric and stenosporic acid constituents. | the antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. both the extracts and the acids showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocol ... | 2006 | 16869486 |
| survival of listeria monocytogenes and enterococcus faecium in sludge evaluated by real-time pcr and culture methods. | this study evaluates the behaviour in spiked sludge of a pathogenic bacteria, listeria monocytogenes, by cultural and molecular techniques, and compares its survival with the one of a faecal indicator, enterococcus faecium. | 2006 | 16869894 |
| effects of acidified sodium chlorite, cetylpyridinium chloride and hot water on populations of listeria monocytogenes and staphylococcus aureus on beef. | the present study was designed to determine the individual and combined effects of acidified sodium chlorite (asc, 0.1%, 24 +/- 1 degrees c), cetylpyridinium chloride (cpc, 0.5%, 24 +/- 1 degrees c) and hot water (hw, 93 +/- 1 degrees c) treatments on the survival of listeria monocytogenes and staphylococcus aureus. | 2006 | 16869900 |
| contribution of penicillin-binding protein homologs to antibiotic resistance, cell morphology, and virulence of listeria monocytogenes egde. | seven open reading frames, annotated as potential penicillin-binding-protein-encoding genes (lmo0441, lmo0540, lmo1438, lmo1892, lmo2039, lmo2229, and lmo2754), were targeted for insertional mutagenesis in listeria monocytogenes egde. these genes were found to contribute in various degrees to beta-lactam resistance, cell morphology, or the virulence potential of this organism. | 2006 | 16870778 |
| safety of etanercept in psoriasis: a critical review. | conventional systemic treatments for patients with psoriasis are associated with multiple adverse effects that require continuous monitoring. the introduction of new biological agents such as etanercept, a fully human fusion protein, has permitted individualisation of patients' treatment according to disease stage. the drug is a competitive inhibitor of tumour necrosis factor-alpha (tnfalpha) that prevents interaction between this cytokine and its cell surface receptors. etanercept also modulate ... | 2006 | 16872241 |
| symbiotic bacteria direct expression of an intestinal bactericidal lectin. | the mammalian intestine harbors complex societies of beneficial bacteria that are maintained in the lumen with minimal penetration of mucosal surfaces. microbial colonization of germ-free mice triggers epithelial expression of regiiigamma, a secreted c-type lectin. regiiigamma binds intestinal bacteria but lacks the complement recruitment domains present in other microbe-binding mammalian c-type lectins. we show that regiiigamma and its human counterpart, hip/pap, are directly antimicrobial prot ... | 2006 | 16931762 |
| [characterization of a recombinant listeria monocytogenes strain containing the fusion protein gene of newcastle disease virus]. | homologous recombination was utilized for construction of a recombinant strain of l. monocytogenes carrying a gene from the newcastle diseases virus by insertional mutation targeting its listeriolysin o gene (hly). the gene encoding fusion protein of the newcastle disease virus (ndv-f) was used as the model heterologous gene. the f gene was inserted into hly downstream to its promoter and signal sequence by overlapping extension polymerase chain reaction, which was then subcloned into the shuttl ... | 2006 | 16933618 |
| listeria monocytogenes food monitoring data and incidence of human listeriosis in hungary, 2004. | food samples were monitored for contamination with listeria monocytogenes, and the incidence of human listeriosis was evaluated according to the data obtained in hungary in the year 2004. of the food samples tested, the bacterium was most often detectable in milk and dairy products, as 72.1% of all l. monocytogenes strains were isolated from these samples. the food samples most commonly yielded strains of serotype 1/2a (45.1%) and 4b (27.0%). in 2004, 3 perinatal and 14 nonperinatal human lister ... | 2006 | 16934896 |
| whole-genome sequence of listeria welshimeri reveals common steps in genome reduction with listeria innocua as compared to listeria monocytogenes. | we present the complete genome sequence of listeria welshimeri, a nonpathogenic member of the genus listeria. listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading frames. comparative genomic analysis of chromosomal regions between l. welshimeri, listeria innocua, and listeria monocytogenes shows strong overall conservation of synteny, with the exception of the translocation of an f(o)f(1) atp synthase. the smaller size of the l. welshimeri genome is the resul ... | 2006 | 16936040 |
| inactivation of adhesion and invasion of food-borne listeria monocytogenes by bacteriocin-producing bifidobacterium strains of human origin. | three bacteriocin-producing bifidobacterial isolates from newborns were identified as bifidobacterium thermacidophilum (two strains) and b. thermophilum (one strain). this study was undertaken to evaluate the ability of these strains to compete with food-borne listeria monocytogenes for adhesion and invasion sites on caco-2 and ht-29 cells. the bifidobacteria adhered at levels ranging from 4% to 10% of the cfu added, but none of the bifidobacteria were able to invade cells. the abilities of list ... | 2006 | 16936051 |
| adoptive transfer of t-cell precursors enhances t-cell reconstitution after allogeneic hematopoietic stem cell transplantation. | immunoincompetence after allogeneic hematopoietic stem cell transplantation (hsct) affects in particular the t-cell lineage and is associated with an increased risk for infections, graft failure and malignant relapse. to generate large numbers of t-cell precursors for adoptive therapy, we cultured mouse hematopoietic stem cells (hscs) in vitro on op9 mouse stromal cells expressing the notch-1 ligand delta-like-1 (op9-dl1). we infused these cells, together with t-cell-depleted mouse bone marrow o ... | 2006 | 16936725 |
| outcome of 8-hour dosing intervals with beta-lactam antibiotics in adult acute bacterial meningitis. | the outcome of dosing with beta-lactam antibiotics at 8-h intervals for adult patients with community-acquired acute bacterial meningitis was retrospectively analysed at sahlgrenska university hospital, gothenburg, sweden. 80 medical records were identified for a 6-y period from 1999 to 2004. the most common pathogens noted were streptococcus pneumoniae (47.5%), neiseria meningitidis (12.5%), and listeria monocytogenes (5.0%). other bacteria were diagnosed in 16.3% of all episodes, but the bacte ... | 2006 | 16938730 |
| neurolisteriosis mimicking herpes simplex encephalitis in an immunocompromized patient. | brain mri was performed in an immunocompromized febrile and lethargic patient, revealing an asymmetric bilateral temporal lobe involvement, strongly suggestive of herpetic encephalitis. nevertheless, pcr in csf for listeria monocytogens was positive. this case implies that listeria might be considered in patients with clinical and imaging suspicion of herpetic encephalitis. | 2006 | 16938743 |
| modulation of phosphoinositide metabolism by pathogenic bacteria. | phosphoinositide metabolism plays a pivotal role in the regulation of receptor-mediated signal transduction, actin remodelling and membrane dynamics. phosphoinositides co-ordinate these processes by recruiting protein effectors to distinct cellular membranes in a time- and organelle-dependent manner. intracellular bacterial pathogens interfere with phosphoinositide metabolism to direct their entry into eukaryotic cells, form replication-permissive vacuoles, modulate apoptosis, or trigger fluid s ... | 2006 | 16939534 |
| vaginal protection and immunity after oral immunization of mice with a novel vaccine strain of listeria monocytogenes expressing human immunodeficiency virus type 1 gag. | natural transmission of human immunodeficiency virus (hiv) occurs at mucosal surfaces. during acute infection, intestinal and other mucosae are preferential sites of virus replication and rapidly become depleted of cd4(+) t cells. therefore, mucosal immunity may be critical to control both initial infection and the massive early spread of virus. an attenuated d-alanine-requiring strain of the oral intracellular microorganism listeria monocytogenes expressing hiv type 1 gag was shown to induce pr ... | 2006 | 16940500 |
| [genes, encoding invasion factors in listeria monocytogenes strains isolated in the european part and the far east of russia]. | the collection of 76 listeria monocytogenes strains isolated from humans, animals and food products was screened with pcr to reveal genes, which encode invasion factors of the internalin family. obtained results demonstrated the correlation between the strain specific polymorphism of the revealed internalin genes and the source of the strain. | 2006 | 16941869 |
| lessons from the organization of a proficiency testing program in food microbiology by interlaboratory comparison: analytical methods in use, impact of methods on bacterial counts and measurement uncertainty of bacterial counts. | the proficiency testing program in food microbiology raema (réseau d'analyses et d'echanges en microbiologie des aliments), created in 1988, currently includes 450 participating laboratories. this interlaboratory comparison establishes proficiency in detection of salmonella and listeria monocytogenes, as well as enumeration of aerobic micro-organisms, enterobacteriaceae, coliforms, beta-glucuronidase-positive escherichia coli, anaerobic sulfito-reducing bacteria, clostridium perfringens, coagula ... | 2006 | 16942983 |
| non-lactic acid, contaminating microbial flora in ready-to-eat foods: a potential food-quality index. | the bacteriological profile of 87 samples of commercially available ready-to-eat (rte) dairy and meat-products, packaged sandwiches and salads was obtained by testing for aerobic colony count, for lactic acid bacterial (lab) count, for the presence and the extent of non-lab microflora (contaminating microflora), and by testing for certain food-borne pathogens. the pathogens listeria monocytogenes, salmonella spp. and sulfite-reducing clostridia were not detected in any of the analysed samples. w ... | 2006 | 16942992 |
| shedding of foodborne pathogens by caenorhabditis elegans in compost-amended and unamended soil. | a study was done to characterize the shedding of foodborne pathogenic bacteria by caenorhabditis elegans, evaluate the persistence of worm populations cocultured with foodborne pathogens, and determine if c. elegans disperses ingested pathogens in soil as a result of shedding. escherichia. coli o157:h7, salmonella enterica serotype poona, and listeria monocytogenes, as well as e. coli op50, a non-pathogenic strain, were studied. synchronous populations of c. elegans were fed for 24 h on confluen ... | 2006 | 16942998 |
| effect of vanillin on the fate of listeria monocytogenes and escherichia coli o157:h7 in a model apple juice medium and in apple juice. | the effects of vanillin on the fates of listeria monocytogenes and escherichia coli o157:h7 at ph values between 3.5 and 4.5 were verified in a model apple juice (maj) medium and in apple juice incubated at 4 or 15 degrees c. viable e. coli o157:h7 cells were recovered from maj for up to 10 days, but l. monocytogenes did not survive at ph 3.5. supplementation with 40 mm vanillin exerted a lethal effect that was species, concentration, ph and temperature dependant. e. coli o157:h7 was more sensit ... | 2006 | 16943001 |
| effect of cold temperature on the composition of different lipid classes of the foodborne pathogen listeria monocytogenes: focus on neutral lipids. | in this work a thorough consideration of the membrane lipid composition of listeria monocytogenes together with dsc analysis is described in order to estimate the biological importance of lipid changes during low-temperature adaptation. furthermore, these studies provide comparative data for fatty acid changes for neutral, nl and polar lipids, pl separately. the cold adaptation (5 degrees c) response of l. monocytogenes showed (i) an increase in the level of nl content (30%) among the total lipi ... | 2006 | 16943003 |
| comparison of pathogenic and spoilage bacterial levels on refrigerated poultry parts following treatment with trisodium phosphate. | this study was undertaken to determine whether trisodium phosphate decontamination of poultry could give a competitive advantage to pathogens and increase microbiological risk to consumers. chicken legs were co-inoculated with similar concentrations of pathogenic (salmonella enteritidis or listeria monocytogenes) and spoilage (pseudomonas fluorescens or brochothrix thermosphacta) bacteria. samples were dipped in tsp (12%, 15 min) or were non-treated (control). microbiological analyses were carri ... | 2006 | 16943004 |
| inhibition of listeria monocytogenes by a lactic acid bacterium isolated from italian salami. | listeria monocytogenes is an opportunistic psychrotroph foodborne pathogen that has been used as a model organism to study the efficacy of many different preservation methods. this work aimed to test the antilisterial activity of lactic acid bacteria isolated from italian salami and study the development of resistance. isolates were obtained from naturally fermented italian salami and cultures that retained activity in the supernatants after ph neutralization and catalase treatment were further ... | 2006 | 16943007 |
| effects of physicochemical surface characteristics of listeria monocytogenes strains on attachment to glass. | seven strains of listeria monocytogenes frequently involved in foodborne disease (epidemic strains) and 14 sporadic strains were examined to compare the attachment and subsequent biofilm growth on glass slides at 37 degrees c. epidemic strains at 3 h incubation had significantly higher attachment values than sporadic strains (p<0.001), but subsequent biofilm growth over 24 h was not dependent on initial attachment. to better understand this phenomenon, the surface hydrophobicity and charge, as w ... | 2006 | 16943011 |
| analyses of the putative crp/fnr family of transcriptional regulators of a serotype 4b strain of listeria monocytogenes. | a whole-genome sequence analysis of listeria monocytogenes strain f2365 revealed 15 potential members of the crp/fnr family of transcriptional regulatory proteins. each gene and the flanking regions were cloned, subjected to in vitro transpositional mutagenesis, and recombined into strain f2365. mutant strains, produced for 14 of the family members, were compared to strain f2365 for differences in carbon utilization, resistance to oxidative stress, and growth under reduced oxygen conditions that ... | 2006 | 16943018 |
| investigation for possible source(s) of contamination of ready-to-eat meat products with listeria spp. and other pathogens in a meat processing plant in trinidad. | in 2003, there was a recall of three processed (chicken franks, spice ham and turkey ham ready-to-eat (rte) meat products by a large processing plant in trinidad as a result of contamination by listeria monocytogenes. the study was conducted to investigate the possible source(s) of listeria contamination of recalled rte meat products and to determine the prevalence of listeria spp., salmonella spp., escherichia coli and campylobacter spp. in the products and air within the plant. raw and process ... | 2006 | 16943025 |
| anti-listerial inhibitory lactic acid bacteria isolated from commercial cold smoked salmon. | the natural microflora of cold-smoked fish at the end of shelf-life are lactic acid bacteria (lab). some of these display a capacity to inhibit spoilage as well as several strains of pathogenic micro-organisms, e.g. listeria monocytogenes which is isolated frequently from cold-smoked salmon (css). eight batches of sliced vacuum-packed css from norway, scotland and spain were collected at retail. packs were stored at 5 degrees c and examined for chemical and microbiological characteristics, at pu ... | 2006 | 16943030 |
| the interaction of the non-bacteriocinogenic lactobacillus sakei 10a and lactocin s producing lactobacillus sakei 148 towards listeria monocytogenes on a model cooked ham. | two lactic acid bacteria, lactobacillus sakei subsp. carnosus (10a) and lactocin s producing lactobacillus sakei 148 (ls5), were examined for their usefulness as protective culture in the biopreservation of cooked meat products. co-culture experiments on a model cooked ham (mch) between 10a or ls5 and a cocktail of three listeria monocytogenes strains were performed to examine the influence of inoculum level (10(5) vs. 10(6)cfu/g), storage temperature (4 vs. 7 degrees c) and packaging type (vacu ... | 2006 | 16943045 |
| survival curves of listeria monocytogenes in chorizos modeled with artificial neural networks. | using artificial neural networks (anns), a highly accurate model was developed to simulate survival curves of listeria monocytogenes in chorizos as affected by the initial water activity (a(w0)) of the sausage formulation, temperature (t), and air inflow velocity (f) where the sausages are stored. the ann-based survival model (r(2)=0.970) outperformed the regression-based cubic model (r(2)=0.851), and as such was used to derive other models (using regression) that allow prediction of the times n ... | 2006 | 16943052 |
| upstream sample processing facilitates pcr detection of listeria monocytogenes in mayonnaise-based ready-to-eat (rte) salads. | sample pretreatment to reduce volume and concentrate cells of the target organism(s) prior to molecular detection offers a useful supplement or alternative to cultural enrichment. the purpose of this study was to develop an upstream processing method to facilitate the detection of listeria monocytogenes in ready-to-eat (rte) salads by pcr. potato salad, a model rte commodity, was seeded with l. monocytogenes and processed by two alternative upstream sample processing methods (designated one-step ... | 2006 | 16943055 |
| antimicrobial properties of salt (nacl) used for the preservation of natural casings. | the antimicrobial properties of salt (nacl) used for the preservation of natural casings were studied by investigating the survival of six bacterial species in natural casings at different water activity (aw) levels. individual sheep casings were inoculated with ca. 10(5) colony-forming units (cfu) g(-1) of escherichia coli, salmonella typhimurium, listeria monocytogenes, staphylococcus aureus, clostridium perfringens and 10(2)cfu g(-1) of e. coli o157:h7. the casings were stored at 20+/-1.5 deg ... | 2006 | 16943065 |
| post-processing application of chemical solutions for control of listeria monocytogenes, cultured under different conditions, on commercial smoked sausage formulated with and without potassium lactate-sodium diacetate. | this study evaluated post-processing chemical solutions for their antilisterial effects on commercial smoked sausage formulated with or without 1.5% potassium lactate plus 0.05% sodium diacetate, and contaminated (approximately 3-4 log cfu/cm(2)) with 10-strain composite listeria monocytogenes inocula prepared under various conditions. inoculated samples were left untreated, or were immersed (2 min, 25 +/- 2 degrees c) in solutions of acetic acid (2.5%), lactic acid (2.5%), potassium benzoate (5 ... | 2006 | 16943080 |
| effect of nisin on growth boundaries of listeria monocytogenes scott a, at various temperatures, ph and water activities. | the effect of nisin on growth boundaries of listeria monocytogenes scott a in tryptone soy broth (tsb) under different a(w)s, ph, and temperatures was studied. growth/no growth turbidity data was modeled using logistic regression. combinations of various temperatures (5-35 degrees c), ph (4.05-6.70) adjusted with hcl, a(w)s (0.937-0.998) nacl (0.5-10.5%) and nisin (0-100 iu/ml) were used to monitor the growth/no growth response of l. monocytogenes scott a for 60 days. the concordance of the logi ... | 2006 | 16943082 |
| control of meatborne listeria monocytogenes and brochothrix thermosphacta by a bacteriocinogenic brochothrix campestris atcc 43754. | the effect of a bacteriocinogenic brochothrix campestris atcc 43754 upon the growth of brochothrix thermosphacta and a 4 strain mixture of listeria monocytogenes was determined in all purpose tween (apt) broth and on pork adipose tissue discs at 4 degrees c. inocula were prepared to give initial numbers of b. campestris of 6-7 log cfu/ml or cm(2) and 3-4 log cfu/ml or cm(2) of b. thermosphacta and l. monocytogenes. adipose tissue discs were evaluated by a sensory panel to determine the intensity ... | 2006 | 16943083 |
| identification of small hfq-binding rnas in listeria monocytogenes. | the rna-binding protein hfq plays important roles in bacterial physiology and is required for the activity of many small regulatory rnas in prokaryotes. we have previously shown that hfq contributes to stress tolerance and virulence in the gram-positive human pathogen listeria monocytogenes. in the present study, we performed coimmunoprecipitations followed by enzymatic rna sequencing to identify hfq-binding rna molecules in l. monocytogenes. the approach resulted in the discovery of three small ... | 2006 | 16682563 |
| identification, subtyping and virulence determination of listeria monocytogenes, an important foodborne pathogen. | listeria monocytogenes is an opportunistic intracellular pathogen that has become an important cause of human foodborne infections worldwide. given its close relationship to other listeria species and its tendency to produce non-specific clinical symptoms, the availability of rapid, sensitive and specific diagnostic tests for the differentiation of l. monocytogenes from other listeria species is helpful for selecting appropriate treatment regimens. in addition, with l. monocytogenes comprising a ... | 2006 | 16687581 |
| escherichia coli interactions with acanthamoeba: a symbiosis with environmental and clinical implications. | the ability of acanthamoeba to feed on gram-negative bacteria, as well as to harbour potential pathogens, such as legionella pneumophila, coxiella burnetii, pseudomonas aeruginosa, vibrio cholerae, helicobacter pylori, listeria monocytogenes and mycobacterium avium, suggest that both amoebae and bacteria are involved in complex interactions, which may play important roles in the environment and in human health. in this study, acanthamoeba castellanii (a keratitis isolate belonging to the t4 geno ... | 2006 | 16687585 |
| surrogates for validation of electron beam irradiation of foods. | the aim of this study was to identify a potential surrogate to describe the radiation sensitivity of the most common pathogens encountered in fruits. three pathogens: escherichia coli o157:h7 933, listeria monocytogenes atcc 51414, and salmonella poona, and five non-pathogens: e. coli k-12 mg1655, listeria innocua seeliger 1983 (nrrl b-33003 and nrrl b-33014), enterobacter aerogenes, and salmonella lt2 were inoculated (populations of 10(7)-10(9) cfu/ml) into model food systems (10% w/w gelatin) ... | 2006 | 16690150 |
| genetic relatedness between listeria monocytogenes isolates from seafood and humans using pfge and rep-pcr. | listeria monocytogenes has been isolated from catfish and various non-catfish seafoods. despite progress that was made to understand the relationship between l. monocytogenes isolated from seafood and humans, no study has emphasized the genetic relatedness between catfish and non-catfish seafood and human isolates. the objectives of this study were to (1) investigate the genetic relationship between l. monocytogenes isolates from catfish, non-catfish seafood and humans using pulsed-field gel ele ... | 2006 | 16690152 |
| efficiency of electrolyzed oxidizing water on reducing listeria monocytogenes contamination on seafood processing gloves. | food processing gloves are typically used to prevent cross-contamination during food preparation. however, gloves can be contaminated with microorganisms and become a source of contamination. this study investigated the survival of listeria monocytogenes on gloves and determined the efficacy of electrolyzed oxidizing (eo) water for reducing l. monocytogenes contamination on seafood processing gloves. three types of reusable gloves (natural rubber latex, natural latex, and nitrile) and two types ... | 2006 | 16690154 |
| dynamic modeling of listeria monocytogenes growth in pasteurized milk. | the development and validation of a dynamic model for predicting listeria monocytogenes growth in pasteurized milk stored at both static and dynamic temperature conditions. | 2006 | 16696676 |
| a comparison of selected methods for measuring the virulence properties of listeria spp. | the comparative ability of different methods to assess virulence of listeria species was investigated in ten listeria strains. all strains were initially subjected to pulsed-field gel electrophoresis analysis to determine their relatedness. virulence characteristics were subsequently tested for by (i) determining the presence of six virulence genes by polymerase chain reaction; (ii) testing for the production of listeriolysin o, phosphatidylcholine phospholipase c, and phosphatidylinositol-speci ... | 2006 | 16699580 |
| fitness of human enteric pathogens on plants and implications for food safety. | the continuous rise in the number of outbreaks of foodborne illness linked to fresh fruit and vegetables challenges the notion that enteric pathogens are defined mostly by their ability to colonize the intestinal habitat. this review describes the epidemiology of produce-associated outbreaks of foodborne disease and presents recently acquired knowledge about the behavior of enteric pathogens on plants, with an emphasis on salmonella enterica, escherichia coli o157:h7, and listeria monocytogenes. ... | 2006 | 16704355 |
| overexpression of prfa leads to growth inhibition of listeria monocytogenes in glucose-containing culture media by interfering with glucose uptake. | listeria monocytogenes strains expressing high levels of the virulence regulator prfa (mutant prfa* or wild-type prfa) show strong growth inhibition in minimal media when they are supplemented with glucose but not when they are supplemented with glucose-6-phosphate compared to the growth of isogenic strains expressing low levels of prfa. a significantly reduced rate of glucose uptake was observed in a prfa*-overexpressing strain growing in lb supplemented with glucose. comparative transcriptome ... | 2006 | 16707681 |
| listeria monocytogenes: a multifaceted model. | the opportunistic intracellular pathogen listeria monocytogenes has become a paradigm for the study of host-pathogen interactions and bacterial adaptation to mammalian hosts. analysis of l. monocytogenes infection has provided considerable insight into how bacteria invade cells, move intracellularly, and disseminate in tissues, as well as tools to address fundamental processes in cell biology. moreover, the vast amount of knowledge that has been gathered through in-depth comparative genomic anal ... | 2006 | 16710323 |
| the specific and essential role of mavs in antiviral innate immune responses. | the mitochondrial antiviral signaling protein (mavs) mediates the activation of nfkappab and irfs and the induction of interferons in response to viral infection. in vitro studies have also suggested that mavs is required for interferon induction by cytosolic dna, but the in vivo evidence is lacking. by generating mavs-deficient mice, here we show that loss of mavs abolished viral induction of interferons and prevented the activation of nfkappab and irf3 in multiple cell types, except plasmacyto ... | 2006 | 16713980 |
| differential requirements for soluble and transmembrane tumor necrosis factor in the immunological control of primary and secondary listeria monocytogenes infection. | the relative contributions of transmembrane tumor necrosis factor (memtnf) and soluble tumor necrosis factor (soltnf) in innate and adaptive immunity are poorly defined. we examined the capacities of wild-type (wt) mice, tnf-/- mice, and memtnf mice, which express only transmembrane tnf, to control primary and secondary listeria monocytogenes infections. soluble tnf was not required for induction or maintenance of protective immunity against a low-dose (200-cfu) listeria infection. in contrast t ... | 2006 | 16714545 |
| interleukin 15-dependent crosstalk between conventional and plasmacytoid dendritic cells is essential for cpg-induced immune activation. | the function of interleukin 15 (il-15) in unmethylated cpg oligodeoxynucleotide (cpg)-induced immune responses remains unknown. here, in response to cpg, both wild-type and natural killer cell-depleted mice produced il-12 and became resistant to a lethal dose of listeria monocytogenes. in contrast, cpg-treated il-15-deficient mice produced little il-12 and succumbed to l. monocytogenes. cpg-stimulated conventional dendritic cells (cdcs) were the main producers of both il-15 and il-12, but cdcs d ... | 2006 | 16715101 |
| mechanism of action of spanish oregano, chinese cinnamon, and savory essential oils against cell membranes and walls of escherichia coli o157:h7 and listeria monocytogenes. | the mechanism of the antimicrobial action of spanish oregano (corydothymus capitatus), chinese cinnamon (cinnamomum cassia), and savory (satureja montana) essential oils against cell membranes and walls of bacteria was studied by the measurement of the intracellular ph and atp concentration, the release of cell constituents, and the electronic microscopy observations of the cells when these essential oils at their mics were in contact with escherichia coli o157:h7 and listeria monocytogenes. e. ... | 2006 | 16715803 |
| inhibitory effect of select nitrocompounds on growth and survivability of listeria monocytogenes in vitro. | we report the effects of 2-nitro-1-propanol (2npoh), 2-nitroethanol (2neoh), and nitroethane (ne) on growth and survivability of listeria monocytogenes. in all cases, inhibition was greatest with 2npoh and least with ne. for example, specific growth rates of l. monocytogenes strain 18 declined (p < 0.05) 76, 60, and 29% from controls during aerobic culture at 37 degrees c in brain heart infusion broth containing 10 mm 2npoh, 2neoh, or ne, respectively. mean specific growth rate for the controls ... | 2006 | 16715805 |
| bacteriocin activity by lactobacillus curvatus cwbi-b28 to inactivate listeria monocytogenes in cold-smoked salmon during 4 degrees c storage. | the inhibition effectiveness of a bacteriocin produced by lactobacillus curvatus cwbi-b28 against listeria monocytogenes was investigated in cold-smoked salmon during storage at 4 degrees c. three bacteriocin-based strategies for the control of l. monocytogenes in foods (i.e., producing bacteriocin in situ, spraying with partially purified bacteriocin, and packaging in bacteriocin-coated plastic film), plus a newly developed method that uses cell-adsorbed bacteriocin (i.e., a suspension of produ ... | 2006 | 16715806 |
| thermal inactivation studies of escherichia coli o157:h7, salmonella, and listeria monocytogenes in ready-to-eat chicken-fried beef patties. | thermal inactivation studies were used to determine the d- and z-values of escherichia coli o157:h7, salmonella, and listeria monocytogenes in ready-to-eat chicken-fried beef patties. inoculated meat was packaged in sterile bags, which were immersed in a circulated water bath and held at 55, 57.5, 60, 62.5, 65, 67.5, and 70 degrees c for different lengths of time. d- and z-values were determined with a linear regression model. average d-values at temperatures 55 to 70 degrees c were 27.62 to 0.0 ... | 2006 | 16715808 |
| pathogen survival in chorizos: ecological factors. | this study addressed health risks from ethnic sausages produced on a small scale, without inspection, in california and elsewhere. mexican-style chorizo, a raw pork sausage that is not cured, fermented, or smoked, was contaminated experimentally in the batter with escherichia coli o157:h7, listeria monocytogenes, or salmonella serotypes and stuffed into natural casings. formulations were based on a market survey in california. physical parameters that were controlled were ph, water activity (a(w ... | 2006 | 16715809 |
| rabbit meat as a source of bacterial foodborne pathogens. | even though worldwide production of rabbit meat is >1,000,000 tons, little information is available for rabbit meat microbiology. this study provides data on the prevalence of salmonella, escherichia coli o157:h7, yersinia enterocolitica, listeria spp., motile aeromonas spp., and staphylococcus aureus on rabbit meat. a total of 24 rabbit carcasses from two abattoirs and 27 rabbit meat packages from supermarket displays were examined. in addition to culturing methods, associated virulence genes w ... | 2006 | 16715811 |
| monitoring the microbial populations and temperatures of fresh broccoli from harvest to retail display. | microbial populations and the temperature of fresh broccoli were monitored at several steps of a supply chain by sampling 33 distinct lots of locally grown produce over two seasons during harvest, storage, wholesale handling, and retail display. imported broccoli was also sampled, but only at retail display. microbiological analyses were conducted on the florets of 201 local and 60 imported broccoli samples to determine populations of total aerobic bacteria (aerobic colony count), fecal coliform ... | 2006 | 16715813 |
| lactobacillus plantarum gene clusters encoding putative cell-surface protein complexes for carbohydrate utilization are conserved in specific gram-positive bacteria. | genomes of gram-positive bacteria encode many putative cell-surface proteins, of which the majority has no known function. from the rapidly increasing number of available genome sequences it has become apparent that many cell-surface proteins are conserved, and frequently encoded in gene clusters or operons, suggesting common functions, and interactions of multiple components. | 2006 | 16723015 |
| control of listeria monocytogenes virulence by 5'-untranslated rna. | the gram-positive bacterium listeria monocytogenes uses a wide range of virulence factors for its pathogenesis. expression of five of these factors has previously been shown to be subjected to post-transcriptional regulation as a result of their long 5'-untranslated region (5'-utr). we have investigated the presence of 5'-utrs among the other known virulence genes and genes that encode putatively virulence-associated surface proteins. our results strongly suggest that l. monocytogenes controls m ... | 2006 | 16730443 |
| listeria pleuritis 1 week after lung transplantation. | listeria monocytogenes is an important bacterial pathogen in immunocompromised patients, the elderly, pregnant women and transplant patients, but until now it has not been reported in lung transplants. we report the first case of listeriosis in a lung transplant recipient who presented with a pleural effusion 8 days after transplantation. after the introduction of a thorax drain and the administration of intravenous antibiotics during 3 weeks, the patient recovered completely. this case highligh ... | 2006 | 16730581 |
| listeriolysin o derived from listeria monocytogenes inhibits the effector phase of an experimental allergic rhinitis induced by ovalbumin in mice. | listeriolysin o (llo) derived from listeria monocytogenes is highly capable of inducing interleukin (il)-12, il-18 and interferon (ifn)-gamma, and facilitates the generation of th1 cells. we have recently shown that recombinant llo (rllo) inhibits generation of ovalbumin (ova)-specific th2 immune response by skewing maturation of antigen-specific t cells into th1 cells. in the present study, we investigated the effect of rllo on the effector phase of th2-dependent allergic rhinitis in balb/c mic ... | 2006 | 16734617 |
| contributions of listeria monocytogenes sigmab and prfa to expression of virulence and stress response genes during extra- and intracellular growth. | listeria monocytogenes sigmab and prfa are pleiotropic regulators of stress response and virulence gene expression. quantitative rt-pcr (qrt-pcr) was used to measure transcript levels of sigmab- and prfa-dependent genes in exponential-phase l. monocytogenes wild-type and deltasigb strains as well as in bacteria exposed to environmental stresses (0.3 m nacl or growth to stationary phase) or present in the vacuole or cytosol of human intestinal epithelial cells. stationary-phase or nacl-exposed l. ... | 2006 | 16735745 |
| panr1, a dominant negative missense allele of the gene encoding tnf-alpha (tnf), does not impair lymphoid development. | a dominant hypomorphic allele of tnf, panr1, was identified in a population of g(1) mice born to n-ethyl-n-nitrosourea-mutagenized sires. macrophages from homozygotes produced no detectable tnf bioactivity, although normal quantities of immunoreactive tnf were secreted. the phenotype was confined to a critical region on mouse chromosome 17, and then ascribed to a c-->a transversion at position 3480 of the tnf gene, corresponding to the amino acid substitution p138t. as a result of subunit exchan ... | 2006 | 16751399 |
| a novel functional t cell hybridoma recognizes macrophage cell death induced by bacteria: a possible role for innate lymphocytes in bacterial infection. | we have established a novel tcralphabeta (tcrvbeta6)(+)cd4(-)cd8(-) t cell hybridoma designated b6ho3. when the b6ho3 cells were cocultured with bacterial-infected j774 macrophage-like cells, ifn-gamma production by b6ho3 cells was triggered through direct cell-cell contact with dying j774 cells infected with listeria monocytogenes (lm), shigella flexneri, or salmonella typhimurium that expressed the type iii secretion system, but not with intact j774 cells infected with heat-killed lm, nonhemol ... | 2006 | 16751404 |
| differential inla and inlb expression and interaction with human intestinal and liver cells by listeria monocytogenes strains of different origins. | in this study, a number of listeria monocytogenes strains of different origins were evaluated for in vitro invasion capacity for various human cell types (monocytic thp-1, enterocytic caco-2, and hepatocytic hepg2 cells) and for expression levels of specific virulence genes. for thp-1 cells, no differences between clinical and nonclinical l. monocytogenes strains in invasion capacity or in production of the proinflammatory cytokine interleukin-8 (il-8) were observed, whereas for the caco-2 and h ... | 2006 | 16751490 |
| a cold-sensitive listeria monocytogenes mutant has a transposon insertion in a gene encoding a putative membrane protein and shows altered (p)ppgpp levels. | a cold-sensitive listeria monocytogenes mutant designated cld-14 was obtained by transposon tn917 mutagenesis. the gene interrupted by tn917 in cld-14 was the l. monocytogenes lmof2365_1485 homolog, which exhibits 45.7% homology to the bacillus subtilis yqff locus. lmof2365_1485, here designated pgph, encodes a putative integral membrane protein with a predicted molecular mass of 81 kda. pgph is predicted to contain a conserved n-terminal signal peptide sequence, seven transmembrane helices, and ... | 2006 | 16751502 |
| one group of genetically similar listeria monocytogenes strains frequently dominates and persists in several fish slaughter- and smokehouses. | contamination of foods with the human pathogen listeria monocytogenes may occur during processing, and the purpose of this study was to determine whether genetically similar strains colonize different processing plants or whether specific persistent strains are unique to each processing plant. we hypothesized that specific l. monocytogenes strains may be better adapted to specific environmental niches in the processing environment. l. monocytogenes contamination patterns were identified by the c ... | 2006 | 16751546 |
| immobilization and detection of listeria monocytogenes. | a procedure was developed for immobilization of listeria monocytogenes cells on metal hydroxides coupled with detection and enumeration using an automated optical system. the results of the immobilization procedure (<1 h) and detection during overnight incubation agreed with calculated plate counts, and this technique is simple and rapid and provides samples that are ready for confirmation of the presence of the pathogen by rapid methods. | 2006 | 16751561 |
| an outbreak of iritis and uveitis in dairy cattle at pasture associated with the supplementary feeding of baleage. | 2006 | 16751847 | |
| way-202196, a selective estrogen receptor-beta agonist, protects against death in experimental septic shock. | to determine the effect of an estrogen receptor-beta selective agent in experimental models of systemic infection and sepsis. | 2006 | 16755255 |
| rapid discrimination of listeria monocytogenes strains by microtemperature gradient gel electrophoresis. | microtemperature gradient gel electrophoresis (mu-tgge) was examined for use for the rapid subtyping of listeria monocytogenes strains. comparison of genomes between l. monocytogenes strains f2365 and h7858 identified a sequence encoding a portion of the prt/pts system iia 2 protein domain as appropriate for mu-tgge analysis. thirty-one strains belonging to 10 different serovar types were tested by pcr, and sequence analysis of the amplified products revealed that the strains comprise 11 groups. ... | 2006 | 16757621 |
| biochemical characteristics of chitosanase from the indonesian bacillus licheniformis mb-2. | bacillus licheniformis mb-2, isolated from a hot spring water in manado, indonesia, secreted a unique chitosanase. media consisted of 0.24% chitosan, 0.25% casiton, 1% mgso4, 1.4% k2hpo4, 0.02% cacl2 x 2h2o, 0.002% feso4 x 7h2o (w/v) was used for enzyme production. purification of the enzyme through the hydrophobic interaction chromatography system (butyl sepharose 4 ff) resulted in two major active fractions; the f2 fraction was shown as a single band at both sodium dodecyl sulfate-polyacrylami ... | 2006 | 16757796 |
| listeria brain abscess, pneumocystis pneumonia and kaposi's sarcoma after temozolomide. | a 55-year-old man with glioblastoma multiforme was treated with continuous, dose-dense temozolomide. this therapy was curtailed after three cycles because of nausea, asthenia, and neuropsychological deterioration. during a subsequent course of radiotherapy, the patient developed fever, headaches, and cutaneous lesions. | 2006 | 16757971 |