Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| sleep deprivation-induced hyperthermia following antigen challenge due to opioid but not interleukin-1 involvement. | eight hours total sleep deprivation does not affect colonic temperature. the combination of a subpyrogenic challenge of sheep red blood cells with sleep loss however, can produce a significant rise in colonic temperature that peaks during the third hour of the sleep deprivation vigil. the regulation of this increase in colonic temperature appears to be opioid in nature and not because of the release of the cytokine interleukin-1. it would appear that the combination of sleep loss and low dose an ... | 1992 | 1317589 |
| the clinical significance of in vitro cytomegalovirus susceptibility to antiviral drugs. | 1992 | 1317603 | |
| some biochemical changes in mouse after in-vivo irradiation of pancreas with ultrasound. | 875 khz continuous wave of ultrasound at 2.5 w/cm2 intensity revealed certain biochemical and enzymological changes in mouse pancreas and liver following the irradiation of pancreas in-vivo for a total of 300 seconds spread over five days. the sacrifice of the animals was carried out on day 0, day 1, day 5 and day 10. blood glucose was reduced significantly with concomitant increase in liver glycogen. glucose-6-phosphatase in liver was decreased significantly while glycogen phosphorylase showed ... | 1992 | 1319155 |
| isolation and molecular characterization of a novel broad-host-range plasmid from bordetella bronchiseptica with sequence similarities to plasmids from gram-positive organisms. | a 2.6 kb plasmid, named pbbr1, was isolated from bordetella bronchiseptica s87. after insertion of an antibiotic resistance marker, this plasmid could be transferred into escherichia coli, bordetella pertussis, b. bronchiseptica, vibrio cholerae, rhizobium meliloti, and pseudomonas putida by transformation or conjugation. conjugation was possible only when the incp group transfer functions were provided in trans. as shown by incompatibility testing, pbbr1 does not belong to the broad-host-range ... | 1992 | 1321324 |
| a homologue of the escherichia coli dsba protein involved in disulphide bond formation is required for enterotoxin biogenesis in vibrio cholerae. | a strain of vibrio cholerae, which had been engineered to express high levels of the non-toxic b subunit (etxb) of escherichia coli heat-labile enterotoxin, was subjected to transposon (tnphoa) mutagenesis. two chromosomal tnphoa insertion mutations of the strain were isolated that showed a severe defect in the amount of etxb produced. the loci disrupted by tnphoa in the two mutant derivatives were cloned and sequenced, and this revealed that the transposon had inserted at different sites in the ... | 1992 | 1324389 |
| a controlled trial of a single dose of azithromycin for the treatment of chlamydial urethritis and cervicitis. the azithromycin for chlamydial infections study group. | currently, there is no single-dose therapy that is effective in the treatment of urethral or endocervical infections with chlamydia trachomatis. azithromycin is a new azalide antibiotic that has substantial activity against c. trachomatis, is concentrated intracellularly, and has a long half-life in serum and tissue. | 1992 | 1325036 |
| 5-ht4 receptor activation induces relaxation and associated camp generation in rat esophagus. | changes in mechanical events and intracellular levels of camp induced by the activation of the 5-ht4 receptor were investigated in the rat esophagus tunica muscularis mucosae preparation. serotonin (5-ht) and 5 methoxytryptamine (5-mot; 5-ht4 agonist) caused concentration-related relaxation responses, while 5-carboxamidotryptamine (5-ct; 5-ht1 agonist), 1-(2,5-dimethoxy-4-iodophenyl)-2-amino-propane (doi; 5-ht2 agonist) and 2-methyl-serotonin (2-methyl-5-ht; 5-ht3 agonist) were less active. the ... | 1992 | 1326439 |
| [resistance of vibrio cholerae 0:1 to the 0/129 compound and multiresistance to antibiotics]. | the authors present a study of the in vitro susceptibility to o/129 compound and usual antibiotics of 29 strains of v. cholerae o:1 biotype el tor isolated during epidemics in miscellaneous countries over the world from 1982 to 1991. several identical isolates from the same epidemic are represented by one strain. susceptibility testing by diffusion method and mics by agar dilution method are used. the data show that the resistance to o/129 compound is often associated with the resistance to usua ... | 1992 | 1327350 |
| possible transduction mechanisms mediating the acute and sensitized response to neurotensin in the ventral tegmental area. | 1992 | 1334385 | |
| possible transduction mechanisms mediating the acute and sensitized response to neurotensin in the ventral tegmental area. | 1992 | 1334385 | |
| interaction of rat peritoneal macrophages with homologous sialidase-treated thrombocytes in vitro: biochemical and morphological studies. detection of n-(o-acetyl)glycoloylneuraminic acid. | sialidase treatment of rat thrombocytes led to an increased binding of these cells to homologous peritoneal macrophages, but had no significant effect on the rate of phagocytosis during the experimental time. as revealed by electron microscopy, the partially desialylated thrombocytes adhere to macrophages predominantly via a small part of the membrane in a way that the discoidal cells adopt a vertical position with regard to the macrophage surface. one adherent macrophage was able to bind up to ... | 1992 | 1334832 |
| oligosaccharide composition of the neurotoxin-responsive sodium channel of mouse neuroblastoma and requirement of sialic acid for biological activity. | a glycoprotein, m(r) 200,000, which has the biological activity of the neurotoxin-responsive na+ channel, was isolated from a clonal line of mouse neuroblastoma cells, n-18. the glycoprotein was purified to homogeneity in 18% yield by methods used to purify glycoproteins, which included metabolic labeling of the cells with l-[3h]fucose and binding of the radioactive glycoproteins to wga- and lentil-sepharose, and deae-cellulose. the glycoprotein has biological activity of neurotoxin-responsive i ... | 1992 | 1337866 |
| [strain of vibrio cholerae resistant to tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole]. | 1992 | 1341805 | |
| [evaluation of household chemical disinfectants for vibrio cholerae el tor (non toxigenic strain)]. | the methodology of microbiological evaluation of disinfectants in permanently being questioned because the laboratorial protocols do not correspond to the real conditions under which these products are used. in 1985 the use-dilution method of aoac was adopted in brazil for microbiological qualification of chemical disinfectants for commercial purposes. domestic disinfectants are tested in this way against salmonella choleraesuis and staphylococcus aureus atcc strains, was chosen for this evaluat ... | 1992 | 1342521 |
| a toxoid prepared from cholera toxin by iodination. | a cholera toxoid was prepared by iodinating purified cholera toxin having an activity of 25 limit of blueing (lb) doses/1 microgram with 0.8 mumol of iodine monochloride per mg toxin, and the residual lesion capacity was tested in mice. the blueing dose (bd) test was strongly positive for the native toxin, and completely abolished in the iodinated toxoid when tested at up to 25 times on lb dose. the dermal microscopic lesions with intradermal doses of 1 microgram virulent toxin presented intense ... | 1992 | 1342838 |
| detection of toxigenic vibrio cholerae 01 using polymerase chain reaction. | 1992 | 1343655 | |
| [evaluation of vibrio cholerae isolation over 10 years in chu fann]. | a retrospective study, concerning ten thousand five hundred and sixty five (10,565) stool samples examined from 1981 to 1990, was done at the bacteriology laboratory of fann university hospital, dakar (sénégal). one thousand and six hundred and eighty (1680) enteropathogen agents were detected (15.9%), five hundred ninety two (592) of which were vibrio cholerae. the quasi totality strains of the vibrio cholerae (99.8%) belonged to the ogawa serotype; they were isolated mainly during the hot and ... | 1992 | 1345081 |
| [preparation and evaluation of coagglutination reagents for the identification of vibrio cholerae 01. its trial during a cholera outbreak in minatitlán, veracruz]. | this study was realized in minatitlán, veracruz during a cholera outbreak. 169 rectal swabs were taken from hilles and their contacts. they were transfer alkaline peptone water for enrichment to v. cholerae and incubated for 8 hrs to 37 degrees c, 70 were positive for v. cholerae in both techniques. the coagglutination was done with a reagent prepared at the instituto de diagnóstico y referencia epidemiológicos of méxico and the culture were also performed in the same institute. we obtained 100% ... | 1992 | 1345312 |
| effect of overproduction of heat shock chaperones groesl and dnak on human procollagenase production in escherichia coli. | the effect of overexpression of the heat shock chaperone genes dnak and groesl on heterologous protein production in escherichia coli was examined, using a set of related human procollagenase proteins. a diverse range of effects on protein solubility, secretion, and accumulation was observed, and these effects were highly dependent on the particular chaperone/procollagenase pairing involved. both chaperones caused a large increase in the apparent solubility of a fusion of the lamb signal peptide ... | 1992 | 1346610 |
| cd2 expression correlates with proliferative capacity of alpha beta + or gamma delta + cd4-cd8- t cells in lpr mice. | the t lymphocytes that accumulate in vast numbers in the lymphoid tissues of lpr/lpr (lpr) mice express a tcr-alpha beta that is polyclonally rearranged, and yet is devoid of surface cd4 or cd8 (cd4-8-) as well as cd2. lpr cd2- alpha beta + cd4-8- t cells exhibit an apparent block in signal transduction, in that when activated they produce little or no il-2 and proliferate minimally in the absence of exogenous il-2. in contrast to the predominant hyporesponsive alpha beta + cd4-8- t cells, we ob ... | 1992 | 1346621 |
| isolation of latin american epidemic strain of vibrio cholerae o1 from us gulf coast. | 1992 | 1347133 | |
| toxigenic vibrio cholerae o1 and cargo ships entering gulf of mexico. | 1992 | 1347134 | |
| conservation of an actinomyces viscosus t14v type 1 fimbrial subunit homolog among divergent groups of actinomyces spp. | the type 1 fimbrial subunit gene of the human actinomyces viscosus t14v was used as a dna probe in southern analyses to detect related dna sequences in 16 of 30 strains of actinomyces spp. under conditions of high stringency. the organisms with homology to the dna probe included two human and six nonhuman a. viscosus, three human and three nonhuman a. naeslundii, and two a. bovis isolates. homologous dna sequences were not detected in strains of a. odontolyticus and a. israelii examined in this ... | 1992 | 1347285 |
| fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection. | thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ... | 1992 | 1347287 |
| symptomatic predictors of ect response in medication-nonresponsive manic patients. | relations between pretreatment symptom ratings and ect outcome were examined in acute manic patients admitted at a state psychiatric center who were nonresponsive to medication. | 1992 | 1347293 |
| il-2 and a contact-mediated signal provided by tcr alpha beta + or tcr gamma delta + cd4+ t cells induce polyclonal ig production by committed human b cells. enhancement by il-5, specific inhibition of iga synthesis by il-4. | to study the role of t cells in t-b cell interactions resulting in isotype production, autologous purified human splenic b and t cells were cocultured in the presence of il-2 and con a. under these conditions high amounts of igm, igg, and iga were secreted. b cell help was provided by autologous cd4+ t cells whereas autologous cd8+ t cells were ineffective. moreover, cd8+ t cells suppressed ig production when added to b cells cocultured with cd4+ t cells. autologous cd4+ t cells could be replace ... | 1992 | 1347306 |
| phenotypic and functional studies on ocular t cells during herpetic infections of the eye. | herpetic stromal keratitis an inflammatory disease of the eye resulting from herpes simplex virus type 1 infection, is a common cause of blindness. the disease is generally considered to represent an immunopathologic response, but the exact mechanism remains in doubt and is subject to debate. we have investigated the nature of inflammatory cells in the eye and have isolated ocular cells to establish their phenotype and determine some of their functions. by means of immunocytochemistry and cytofl ... | 1992 | 1347309 |
| effects of in vivo t lymphocyte subset depletion on mycobacterial infections in mice. | the relative importance of cd4+ and cd8+ t cell subsets in the expression of acquired resistance to systemic infection by mycobacterium kansasii was determined. t cell subsets were depleted in thymectomized c57bl/6 mice by the intravenous administration of monoclonal antibodies directed against the relevant t cell determinants. depletion of the cd4+ subset exacerbated the severity of the infection in intravenously challenged mice. this effect was apparent in the first 2 weeks of the infection an ... | 1992 | 1347311 |
| porcine skeletal muscle myofibrillar protein synthesis is stimulated by ractopamine. | thirty-two 64-kg individually penned barrows were fed protein at 130 or 170 g/kg diet and ractopamine at 0 or 20 mg/kg in a randomized complete block design for 28 d. fractional synthesis rates of myofibrillar, sarcoplasmic and connective tissue proteins in longissimus dorsi, biceps femoris and gastrocnemius muscles were examined by primed-continuous infusion of l-[ring-2,6-3h(n)]phenylalanine over a 4-h period. fractional synthesis, accretion and breakdown rates of protein in the muscles were n ... | 1992 | 1347314 |
| [h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors]. | among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ... | 1992 | 1347325 |
| genetic relation between vibrio cholerae o1 strains in ecuador and bangladesh. | 1992 | 1347603 | |
| vibrio cholerae non-o1 in sewage lagoons and seasonality in peru cholera epidemic. | 1992 | 1348337 | |
| cholera diagnosed in clinical laboratory by dna hybridisation. | 1992 | 1348815 | |
| symptomless carriage of vibrio cholerae in peru. | 1992 | 1349080 | |
| role of human t-cell leukemia virus type 1 x region proteins in immortalization of primary human lymphocytes in culture. | human t-cell leukemia virus type 1 (htlv-1) immortalizes human cd4+ t lymphocytes in culture. previous studies show that in the context of a herpesvirus saimiri vector, the sequence of the x region at the 3' end of the htlv-1 genome is also capable of immortalizing cd4+ lymphocytes in the absence of htlv-1 structural proteins. the x region of htlv-1 encodes two trans-acting viral proteins, the 42-kda tax protein and the 27-kda rex protein. infection of human cord blood cells with herpesvirus sai ... | 1992 | 1351105 |
| hut 78 t cells bind to noncytokine-stimulated keratinocytes using a non-cd18-dependent adhesion pathway. | the initial in vitro observation that cultured keratinocytes, when treated with cytokines such as gamma interferon, increased the binding of t lymphocytes, opened up a whole new avenue of research to understand epidermal trafficking patterns in inflammatory skin diseases. a growing body of data strongly supports the in vivo role of lymphocyte-function-associated antigen-1 (cd18) expression by t cells in the binding to intercellular adhesion molecule-1 (cd54) expressing keratinocytes. to further ... | 1992 | 1351368 |
| of cabbages and chlorine: cholera in peru. | the low case fatality rates (1%) from the 1991 cholera epidemic in peru was more a result of including diarrheas of a less virulent etiology than that of cholera. in fact, a study during the early phases of the cholera epidemic in trujillo, peru revealed that only 79% of suspected cholera cases were infected with vibrio cholera 01. further other people contended that the government of peru did not chlorinate many water supplies because studies by the us environmental protection agency suggeste ... | 1992 | 1351603 |
| waterborne transmission of epidemic cholera in trujillo, peru: lessons for a continent at risk. | the epidemic of cholera that began in peru in january, 1991, marked the first such epidemic in south america this century. subsequently, over 533,000 cases and 4700 deaths have been reported from nineteen countries in that hemisphere. we investigated the epidemic in trujillo, the second largest city in peru. trujillo's water supply was unchlorinated and water contamination was common. suspect cholera cases were defined as persons presenting to a health facility with acute diarrhoea between feb 1 ... | 1992 | 1351608 |
| optimal management strategies for hiv-infected patients who present with cough or dyspnea: a cost-effective analysis. | to determine the effectiveness and costs of alternative management strategies for patients infected with the human immunodeficiency virus (hiv) who present with pulmonary symptoms. | 1992 | 1351940 |
| interaction of heat-stable enterotoxins with human colonic (t84) cells: modulation of the activation of guanylyl cyclase. | heat-stable enterotoxins (st) activate guanylyl cyclase in t84 cells, rapidly and specifically. activation is monitored by cgmp production and occurs at lower concentrations of st than required for eliciting fluid accumulation in suckling mice. atrial natriuretic factor (anf) neither activates guanylyl cyclase nor modulates the response to st in t84 cells, indicating the absence of receptors for anf on t84 cells. monitoring the production of cgmp under conditions known to alter fluid accumulatio ... | 1992 | 1352030 |
| homology of tcpn, a putative regulatory protein of vibrio cholerae, to the arac family of transcriptional activators. | the nucleotide sequence has been determined for the gene designated tcpn, encoding a putative regulatory protein within the tcp gene cluster associated with the biosynthesis and assembly of the toxin-coregulated pilus of vibrio cholerae. it is preceded by a powerful transcriptional terminator which presumably delimits the major tcp operon, but at its 3' end is translationally coupled to the gene, tcpj, encoding the tcp pilin signal peptidase. the tcpn gene encodes a putative 276-residue protein ... | 1992 | 1352761 |
| emergence of vibrio cholerae o1 resistant to vibriostatic agent 0/129. | 1992 | 1353821 | |
| characterization of serpulina (treponema) hyodysenteriae and related intestinal spirochetes by ribosomal rna gene restriction patterns. | serpulina (treponema) hyodysenteriae strain a-1 partially purified rrna, labelled with photobiotin, was used as a non-radioactive probe to identify the rrna gene restriction patterns of s. hyodysenteriae strains and other spirochetes. sau3a restriction enzyme digests resulted in similar rrna gene restriction patterns in s. hyodysenteriae strains from five different countries. some s. hyodysenteriae strains could be differentiated by variations in their rrna gene restriction patterns after cleava ... | 1992 | 1354196 |
| pulo, a component of the pullulanase secretion pathway of klebsiella oxytoca, correctly and efficiently processes gonococcal type iv prepilin in escherichia coli. | the pulo protein required for extracellular secretion of pullulanase by klebsiella oxytoca is known to be highly homologous to two type iv prepilin peptidases, namely xcpa(pild) (pseudomonas aeruginosa) and tcpj (vibrio cholerae). the predicted prepilin peptidase activity of pulo was confirmed by showing that it could correctly process the product of the cloned pile.1 type iv pilin structural gene from neisseria gonorrhoeae in escherichia coli. the p. aeruginosa prepilin peptidase and another pu ... | 1992 | 1354833 |
| safety and immunogenicity of single-dose live oral cholera vaccine cvd 103-hgr in 5-9-year-old indonesian children. | oral vaccines offer great promise as public-health measures to prevent disease in less-developed countries. cvd 103-hgr, a genetically engineered, attenuated, vibrio cholerae o1 strain has proved effective in industrialised countries. we have assessed the safety, immunogenicity, and excretion of this live cholera vaccine in children in north jakarta, indonesia. 412 children aged 5-9 years received single doses of 5 x 10(6), 5 x 10(7), 5 x 10(8), 5 x 10(9), or 1 x 10(10) colony forming units (cfu ... | 1992 | 1355798 |
| chlorine and survival of "rugose" vibrio cholerae. | 1992 | 1355849 | |
| overexpression of p-glycoprotein and alterations in topoisomerase ii in p388 mouse leukemia cells selected in vivo for resistance to mitoxantrone. | the overexpression of p-glycoprotein (pgp) and alterations in dna topoisomerase ii (topo ii) were evaluated in mouse leukemia p388 cells selected in vivo for mitoxantrone (mtt) resistance (p388/mtt) and compared to doxorubicin (dox) resistant (p388/dox) or vincristine (vcr) resistant (p388/vcr) models. among a panel of topo ii inhibitors which included etoposide (vp-16), dox, mtt and 4'-[(9-acridinyl)-amino]methanesulfon-m-anisidide (m-amsa), the relative resistance compared to parental sensitiv ... | 1992 | 1356339 |
| interferon gamma but not tumor necrosis factor alpha decreases susceptibility of human renal cell cancer cell lines to lymphokine-activated killer cells. | human renal cell cancer (rcc) cell lines, achn and krc/y, with or without exposure to cytokines, were examined for their susceptibility to lymphokine-activated killer (lak) cells. flow-cytometric analysis demonstrated constitutional expression of class i antigen on both cell lines, which was enhanced by interferon alpha (ifn alpha), ifn gamma and tumor necrosis factor alpha (tnf alpha). a 4-h 51cr-release cytotoxicity assay demonstrated that pretreatment of both cell lines with ifn gamma or ifn ... | 1992 | 1356627 |
| the fimbriae-like structures on v. cholerae isolated in kenya. | for vibrio cholerae 01 to overcome the normal intestinal clearing mechanisms and facilitate colonization in the host gut, fine filamentous structures covering the surface of all the cell must exist. these fimbriae-like structures were investigated in this study. selection of the k23 v. cholerae 01 biotype el-tor isolated in kisumu, kenya, was based on its proteinase activity and ability to agglutinate 0.2m ammonium sulphate. this strain was cultured in modified tryptone broth ph 8.4, at 37 degre ... | 1992 | 1356750 |
| analysis of expression of toxin-coregulated pili in classical and el tor vibrio cholerae o1 in vitro and in vivo. | the expression of toxin-coregulated pili (tcp) and their structural subunit tcpa was compared in 20 strains of vibrio cholerae of the classical and el tor biotypes. bacteria were isolated from the intestines of rabbits with experimental cholera and compared with the same strains grown under optimal tcp expression conditions in vitro. immunoblotting revealed that tcpa production was induced in both biotypes after vibrios entered the intestinal milieu; tcpa-negative inocula gave rise to tcpa-posit ... | 1992 | 1356927 |
| protection against vibrio cholerae el tor infection by specific antibodies against mannose-binding hemagglutinin pili. | both specific polyclonal antiserum and monoclonal antibodies against mannose-binding hemagglutinin fimbriae of vibrio cholerae (mannose-sensitive hemagglutinin [msha]) were shown to protect against experimental cholera caused by vibrios of the el tor biotype in the infant mouse and in the rabbit intestinal loop models. msha-specific fab immunoglobulin fragments were also protective. no protective effect was observed against challenge with v. cholerae o1 of the classical biotype. these results su ... | 1992 | 1356934 |
| vibrio cholerae o1 septicaemia. | 1992 | 1357316 | |
| disinfection of water by sunlight. | 1992 | 1357340 | |
| tcp pilus biosynthesis in vibrio cholerae o1: gene sequence of tcpc encoding an outer membrane lipoprotein. | the nucleotide sequence of the tcpc gene has been determined. it encodes a 53995-da protein precursor with a signal sequence and cleavage site typical of a number of outer membrane lipoproteins, which are cleaved by the equivalent of signal peptidase ii (lsp) of escherichia coli. the location of the tcpc gene is such that it is predicted to be translationally coupled to the 5' and 3' flanking genes, tcpy and tcpd, respectively, indicating that it forms part of an operon. together with the lipopr ... | 1992 | 1358749 |
| comparison of sodium transport processes of human and rat erythrocytes in hypertension. | erythrocytes of normotensive and hypertensive humans, as well as sprague-dawley, wistar-kyoto and spontaneously hypertensive rats, were prepared to have similar ionic compositions adequate for determining the activities of lithium-sodium (li-na) countertransport and sodium (na) pump. the rate of li-na countertransport was significantly higher in erythrocytes of hypertensive subjects. this activity was not detected in rat erythrocytes, at two different ages, and over a six-fold of lithium (li) co ... | 1992 | 1360293 |
| pregnancy after transfer of frozen-thawed human embryos. | cryopreservation of human embryos has been successfully applied in in vitro fertilization (ivf) and embryo transfer (et) programs at the national taiwan university hospital since 1988. our preliminary results with 120 frozen-thawed embryos in 31 transfer cycles showed that the survival rate of frozen embryos was 66%. following transfer, the implantation rate and clinical pregnancy rate were 6.5% and 13%, respectively. four clinical pregnancies and one preclinical pregnancy following a frozen-tha ... | 1992 | 1360300 |
| the role of nerve growth factor in collateral reinnervation by cutaneous c-fibres in the rat. | we have investigated whether chronic nerve growth factor (ngf) depletion affects the development of transmedian collateral reinnervation by c-fibres. using a dye-labelled plasma extravasation technique in rats, the extent of transmedian innervation of the skin by c-fibres in the inferior alveolar nerve (ian) was determined 8-10 weeks after sectioning and preventing regeneration of the contralateral ian. another group of animals were immunised against ngf prior to the nerve section and a third gr ... | 1992 | 1360319 |
| trans-acpd-induced phosphoinositide hydrolysis and modulation of hippocampal pyramidal cell excitability do not undergo parallel developmental regulation. | the selective metabotropic glutamate receptor agonist, trans-1-aminocyclopentane-1,3-dicarboxylic acid (trans-acpd), stimulates phosphoinositide hydrolysis and elicits a number of electrophysiological responses in the hippocampus. if these effects are mediated by the same receptor subtype, they should undergo parallel developmental regulation. therefore, we compared the phosphoinositide hydrolysis response and the electrophysiological responses to trans-acpd at two different developmental stages ... | 1992 | 1360322 |
| chromatographic and immunological characterisation of neuropeptide y-like and pancreatic polypeptide-like peptides from the nematode ascaris suum. | 1. immunoreactivity (ir) towards neuropeptide y (npy) and pancreatic polypeptide (pp) has previously been demonstrated in nematodes by immunocytochemistry (icc). 2. the pp- and npy-ir in the nematode parasite ascaris suum were analysed using hplc and radioimmunoassays (rias) specific for either peptide. 3. quantitative ria data on the distribution of these irs in the parasite disagreed with icc data suggesting that different tissue treatments used in the two techniques lead to different antigen ... | 1992 | 1360356 |
| inhibitory effect of a microcystis sp (cyanobacteria) toxin on development of preimplantation mouse embryos. | 1. a soluble toxin, purified from the algae bloom of an eutrophic lake dominated by microcystis, is a very effective inhibitor of early embryo development in a dose-response relationship. 2. two- and 8-cell mouse embryos under the influence of microcystis toxin do not reach the developmental stages of morula and blastocyst, respectively. 3. actin cortex is disorganized without change in the microtubules structure. 4. results are discussed in terms of the possible mechanisms by which the toxin ar ... | 1992 | 1360361 |
| bq-153, a novel endothelin (et)a antagonist, attenuates the renal vascular effects of endothelin-1. | endothelin (et)-1, leukotriene d4 and the thromboxane analogue, u-44069, were all shown to produce dose-dependent reductions in renal blood flow after direct injection into the renal artery of anaesthetized pigs. the effects of et-1 differed from the other two mediators in that et-1 caused a transient vasodilator followed by a prolonged vasoconstrictor response. the pressor response was not mediated by the secondary release of either leukotriene d4 or thromboxane a2 as evidenced by the lack of e ... | 1992 | 1360536 |
| molecular design of cholera vaccines. | cholera is still a serious public health problem in developing countries, particularly those in tropical regions. this has stimulated considerable research into the molecular analysis of pathogenesis resulting in the identification of a number of critical components required for both colonization of the gut mucosa and the disease symptoms. these components are the targets for rational molecular approaches to vaccine development. | 1992 | 1361701 |
| enzyme-linked immunosorbent assay for determination of antibodies to vibrio cholerae toxin-coregulated pili. | an elisa for determination of antibodies to v. cholerae tcp was developed. since purified tcp preparations contained detectable amounts of lps (as shown by elisa and immunoelectron microscopy with anti-lps polyclonal serum), a capture elisa was used. in this test the plate was coated with anti-tcp monoclonal antibody followed by incubation with tcp fimbriae. by this procedure no lps bound to the solid phase as shown by the loss of reactivity with anti-lps serum. the capture elisa allowed sensiti ... | 1992 | 1361754 |
| [vibrio cholerae toxin b subunit gene expressed in a salmonella vaccine strain]. | this paper reports that the v. cholerae toxin b subunit (ctx b) gene was inserted into pya 248 plasmid with the aspartate beta-semialdehyde dehydrogenase (asd)gene and the recombinant plasmid was transformed into s. typhimurium deleting asd gene. results showed that ctx b gene was highly expressed and secreted into midium. this strain was able to colonize in the intestinal epithelium. oral immunity and general immunity could produce antibodies at high level and enhance cellular immune responses. ... | 1992 | 1362309 |
| the antibody response to an oral ty21a-based typhoid-cholera hybrid is unaffected by prior oral vaccination with ty21a. | 1992 | 1727891 | |
| role of vibrio cholerae neuraminidase in the function of cholera toxin. | vibrio cholerae neuraminidase (nanase) is hypothesized to act synergistically with cholera toxin (ct) and increase the severity of a secretory response by increasing the binding and penetration of ct to enterocytes. to test this hypothesis, the nanase gene (nanh) from v. cholerae ogawa 395 was first cloned and sequenced. isogenic wild-type and nanase- v. cholerae 395 strains were then constructed by using suicide vector-mediated mutagenesis. the influence of nanase on ct binding and penetration ... | 1992 | 1730470 |
| cloning of a gene (zot) encoding a new toxin produced by vibrio cholerae. | live oral candidate cholera vaccines have previously been constructed by deletion of vibrio cholerae sequences encoding the enzymatically active a subunit of the cholera toxin. however, volunteer studies have shown that these non-cholera toxin-producing strains still provoke mild to moderate diarrhea in some individuals. we recently reported the identification of a second toxin produced by v. cholerae which may be responsible for this residual diarrhea (a. fasano, b. baudry, d. w. pumplin, s. s. ... | 1992 | 1730472 |
| non-o1 vibrio cholerae intestinal pathology and invasion in the removable intestinal tie adult rabbit diarrhea model. | a modified removable intestinal tie adult rabbit diarrhea (ritard) model was used to investigate the intestinal pathology, intestinal bacterial colonization, intestinal fluid volume, and onset of diarrhea caused by non-o1 vibrio cholerae. three strains of non-o1 v. cholerae were studied. ritard rabbits challenged with 10(3) cfu of strain nrt36s (a strain previously shown to cause diarrhea in volunteers) developed grade 3 diarrhea at 48 to 72 h. the mean counts of non-o1 v. cholerae isolated were ... | 1992 | 1730473 |
| vibrio cholerae hemagglutinin/protease, colonial variation, virulence, and detachment. | the structural gene, hap, for the secreted hemagglutinin/protease (ha/protease), a putative virulence factor of vibrio cholerae, has recently been cloned and sequenced (c. c. häse and r. a. finkelstein, j. bacteriol. 173:3311-3317, 1991). the availability of the null mutant, hap-1, and hap-1 complemented with pch2 (which expresses ha/protease), enabled an examination of the role of ha/protease in the virulence of v. cholerae in an animal model. however, the mutants exhibited reversible colonial ... | 1992 | 1730478 |
| occurrence of resistance to vibriostatic compound 0/129 in vibrio cholerae 01 isolated from clinical and environmental samples in bangladesh. | fifty-one vibrio cholerae 01 strains isolated from 734 natural water and plankton samples and 31 rectal swabs were examined. of these strains, 32 (62.7%) were found to be resistant to vibriostatic compound 0/129. when antibiograms using the antibiotics ampicillin, tetracycline, chloramphenicol, trimethoprim-sulfamethoxazole, furoxan, and gentamicin were done, it was observed that there was a correlation of sensitivity to 0/129 with selected antibiotics. only the ogawa e1 tor (72% of strains resi ... | 1992 | 1734056 |
| cholera associated with an international airline flight, 1992. | 1992 | 1738360 | |
| nucleotide and deduced amino acid sequence of the reca gene of vibrio cholerae. | 1992 | 1741267 | |
| [characterization of monoclonal antibodies that recognize the thermolabile toxin of vibrio cholerae]. | the obtention of two monoclonal antibodies which recognize a single epitope present in the subunit b of the thermolabile toxin of vibrio cholerae and another which shows a cross-reaction with those produced by certain enteropathogenic toxins, is reported. the standardization of a solid phase indirect immunoenzymatic assay (elisa) for the primary screening and selection of hybrids was performed; in addition, the isotype was determined. | 1992 | 9768213 |
| effects of msh/acth 4--10 on memory, attention and endogenous hormone levels in women. | 1992 | 205892 | |
| mercury complexes of thionicotinamide adenine dinucleotide. | one-to-one mercury complexes of thionicotinamide adenine dinucleotide (tnad+) were prepared by using hgso4 and hg(ch3 coo-)2. optical absorption spectroscopy indicated that the mercury probably binds to the tnad+ through the thio-keto group on the pyridine ring. x-ray diffraction patterns of crystals of mitochondrial malate dehydrogenase soaked in solution containing tnad+ . mercury complex indicated binding and the x-ray intensity differences are different from mercurials alone. | 1992 | 207356 |
| a two-rooted maxillary central incisor. | 1992 | 265496 | |
| medical manpower. | 1992 | 265454 | |
| stress due to nuclear accident: a survey of an employee population. | 1992 | 257251 | |
| [physiological bases of the sleep-wakefulness rhythm]. | 1992 | 206063 | |
| [radiological lacuna in the 2d part of the duodenum (pseudo-ampulloma) caused by ampullar calculi and distension of the lower bile duct. significance of duodenoscopy with biliary and pancreatic catheterization]. | 1992 | 189414 | |
| letter: obesity, diet, and type-ii hyperlipidaemia. | 1992 | 48702 | |
| growth factors for tumours. | 1992 | 205794 | |
| [mandibular reconstruction with reference to joint and nerve substitution]. | 1993 | 281312 | |
| [free bone transplant as mandibular substitute with special reference to chin reconstruction]. | 1993 | 281311 | |
| [di gugliemo's syndrome (erythroleukemia)]. | 1993 | 266223 | |
| gold therapy in rheumatoid arthritis. | 1993 | 1679184 | |
| serum steroid hormones and pituitary function in female epileptic patients during carbamazepine therapy. | ten regularly menstruating women with epilepsy were studied in a 12-month prospective follow-up study to evaluate the short-term effects of carbamazepine (cbz) on serum sex hormone balance and pituitary function. thirteen female epilepsy patients receiving long-term cbz monotherapy (mean medication duration 5.3 years) were also studied. controls were 17 regularly menstruating healthy volunteers. untreated patients had higher free testosterone (ft) and luteinizing hormone (lh) serum concentration ... | 1993 | 2142450 |
| [stannous fluoride and amine hydrofluorides in the prevention of dental caries in adults. results of a 3-year follow-up study]. | 1993 | 265780 | |
| [plak-out and broxojet 3007. clinical study]. | it is known that chlorhexidine, a plaque preventing agent, can cause discolorations and other side effects when used in mouthwashes at the concentration of 0.1% or more. by diluting the amount of chlorhexidine used in a mouthwash in the much greater volume of water used in a pulsating device, one could try to avoid these side-effects and, possible keep the antiplaque properties of chlorhexidine. furthermore, it was interesting to check whether plaque, which is normally not removed by the mechani ... | 1993 | 280956 |
| [results of x-ray therapy and radioisotope teletherapy of laryngeal cancer]. | 1993 | 1134242 | |
| post-jejunoileal-bypass hepatic disease. its similarity to alcoholic hepatic disease. | the authors studied serial hepatic biopsies of five patients who developed hepatic failure following jejunoileal bypass for extreme obesity, with autopsies of two. the hepatic histologic changes included centrilobular or focal alcoholic hyalin, intrasinusoidal collagenosis, fatty hydropic degeneration, and neutrophilic infiltrate. at least two of the patients were abstinent from alcohol, both prior to and after the surgical procedures. the others, after the bypass procedures, had reduced alcohol ... | 1993 | 46697 |
| [reflections on medico-legal activities at two university-medical centers]. | 1993 | 262926 | |
| new approach to management of intracranial aneurysms. | in six cases an attempt was made to relieve the tension on intracranial aneurysms by temporarily clamping the internal carotid artery in the neck, so as to increase the expansibility of the artery. this approach was based on the concept (or "a principle") that haemorrhage is caused by the aneurysm having to bear the full force of systolic pulse pressure when atherosclerosis prevents this pressure being taken up by the normally expansile arterial wall. follow-up has been fairly short, but the pre ... | 1993 | 46379 |
| [dorsal dislocation of the trapezoid and second metacarpal bones. review of literature and case report]. | 1993 | 262857 | |
| vibrio cholerae in disguise-a disturbing entity. | 1993 | 24420105 | |
| detection of vibrio cholerae o1 in the aquatic environment in brazil employing direct immunofluorescence microscopy. | culturing and immunofluorescence (fa) methods for detection of vibrio cholerae o1 in samples collected from the aquatic environment at selected sites in brazil were compared. of the samples examined, 90% were positive for v. cholerae o1 by fa but none was positive by culture, although strains of v. cholerae other than o1 strains were readily isolated. evidence for v. cholerae o1 being autochthonous to the aquatic environment of brazil is presented. furthermore, fa methods are recommended for cho ... | 1993 | 24420052 |
| large-scale production of vibrio cholerae toxin b subunit for use in oral vaccines. | by systematically manipulating promoter and ribosome binding structures, plasmid copy number and the structure of the cholera toxin b (ctb) subunit gene, we were able to develop a plasmid expression system that, when used in conjunction with an optimized growth medium, provided yields of ctb approaching one gram per liter. the ctb protein which was secreted to > 95%, could readily be purified from the growth medium of a v. cholerae production strain and was shown to be immunologically indistingu ... | 1993 | 7764248 |
| bacterial response to host signals: analysis and applications. | 1993 | 7622356 | |
| use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit b in vibrio cholerae o1 strains. | cholera toxin is the principal factor causing the profuse intestinal fluid secretion that is characteristic of cholera. the dna sequences of the cholera toxin subunit b structural genes from 45 vibrio cholerae o1 strains isolated in 29 countries over a period of 70 years were determined by automated dna sequencing of polymerase chain reaction-generated amplicons. three types of cholera toxin b subunit gene (ctxb) were identified. genotype 1 was found in strains of classical biotype worldwide and ... | 1993 | 7678018 |
| cloning and genetic analysis of the vibrio vulnificus fur gene and construction of a fur mutant by in vivo marker exchange. | vibrio vulnificus infections have been associated with iron overload and preexisting liver disease. iron may play a major role in the pathogenesis of v. vulnificus infections. many virulence genes, as well as genes involved in the transport of iron by bacteria, are regulated by iron, with increased expression under low-iron conditions. in escherichia coli and vibrio cholerae, transcriptional regulation by iron depends on the fur gene. we utilized southern hybridization under low- and high-string ... | 1993 | 7678593 |