Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| transmembrane segments of complement receptor 3 do not participate in cytotoxic activities but determine receptor structure required for action of bordetella adenylate cyclase toxin. | adenylate cyclase toxin-hemolysin (cyaa, act or ac-hly) of the whooping cough agent bordetella pertussis penetrates phagocytes expressing the integrin complement receptor 3 (cr3, cd11b/cd18, α(m)β(2) or mac-1). cyaa translocates its adenylate cyclase (ac) enzyme domain into cell cytosol and catalyzes unregulated conversion of atp to camp, thereby subverting cellular signaling. in parallel, cyaa forms small cation-selective membrane pores that permeabilize cells for potassium efflux, contributing ... | 2016 | 26802078 |
| reciprocal interference of maternal and infant immunization in protection against pertussis. | because of the current re-emergence of pertussis, vaccination during the 3rd trimester of pregnancy is recommended in several countries in order to protect neonates by placental transfer of maternal antibodies. here, we examined the potential reciprocal interference of mother and infant vaccination in protection against pertussis in mice. | 2016 | 26776471 |
| biofilm forming potential and antimicrobial susceptibility of newly emerged western australian bordetella pertussis clinical isolates. | whooping cough caused by bordetella pertussis is increasing in several countries despite high vaccine coverage. one potential reason for the resurgence is the emergence of genetic variants of the bacterium. biofilm formation has recently been associated with the pathogenesis of b. pertussis. biofilm formation of 21 western australian b. pertussis clinical isolates was investigated. all isolates formed thicker biofilms than the reference vaccine strain tohama i while retaining susceptibility to a ... | 2016 | 27669900 |
| highlights of the 11th international bordetella symposium: from basic biology to vaccine development. | pertussis is a severe respiratory disease caused by infection with the bacterial pathogen bordetella pertussis the disease affects individuals of all ages but is particularly severe and sometimes fatal in unvaccinated young infants. other bordetella species cause diseases in humans, animals, and birds. scientific, clinical, public health, vaccine company, and regulatory agency experts on these pathogens and diseases gathered in buenos aires, argentina from 5 to 8 april 2016 for the 11th internat ... | 2016 | 27655886 |
| globin domain interactions control heme pocket conformation and oligomerization of globin coupled sensors. | globin coupled sensors (gcs) are o2-sensing proteins used by bacteria to monitor the surrounding gaseous environment. to investigate the biphasic o2 dissociation kinetics observed for full-length gcs proteins, isolated globin domains from pectobacterium carotovorum ssp. carotovorum (pccglobin), and bordetella pertussis (bpeglobin), have been characterized. pccglobin is found to be dimeric, while bpeglobin is monomeric, indicating key differences in the globin domain dimer interface. through char ... | 2016 | 27614715 |
| pertussis leukocytosis: mechanisms, clinical relevance and treatment. | the significant and sometimes dramatic rise in the number of circulating white blood cells (leukocytosis) in infants suffering from pertussis (whooping cough) has been recognized for over a century. although pertussis is a disease that afflicts people of all ages, it can be particularly severe in young infants, and these are the individuals in whom leukocytosis is most pronounced. very high levels of leukocytosis are associated with poor outcome in infants hospitalized with pertussis and modern ... | 2016 | 27609461 |
| synergistic neutralization of pertussis toxin by a bispecific antibody in vitro and in vivo. | bispecific antibodies are a rapidly growing class of therapeutic molecules, originally developed for the treatment of cancer but recently explored for the treatment of autoimmune and infectious diseases. bordetella pertussis is a reemerging pathogen, and several of the key symptoms of infection are caused by the pertussis toxin (ptx). two humanized antibodies, hu1b7 and hu11e6, bind distinct epitopes on ptx and, when coadministered, mitigate disease severity in murine and baboon models of infect ... | 2016 | 27581436 |
| [insertional inactivation of virulence operon in population of persistent bordetella pertussis bacteria]. | avirulent b. pertussis bacteria containing is elements in the bvgas operon were detected during the study of whooping cough patients and bacilli carriers. the present work is devoted to the study of the accumulation dynamics and the mechanisms of generation of persistent forms of the b. pertussis bacteria in lower monkeys as the most adequate model for extrapolation ofthe experiment results to humans. by means of the real-time pcr method, it was established that the b. pertussis bacteria lived m ... | 2016 | 27529975 |
| [serological evaluation of bordetella pertussis infection in adults with prolonged cough]. | pertussis is a vaccine-preventable disease that is transmitted from infected to susceptible individuals by respiratory route. bordetella pertussis infection may occur at any age as neither vaccine nor natural infection induced immunity lasts life-long. this study was planned to demonstrate the serological evidence of infection among adults, to raise awareness among clinicians and to provide data for the development of strategies to protect vulnerable infants. a total of 538 patients (345 female, ... | 2016 | 27525392 |
| viral etiology in adult influenza-like illness/acute respiratory infection and predictivity of c-reactive protein. | influenza-like illness (ili) and acute respiratory infection (ari) are common presentations during winter and indiscriminate antibiotic use contributes significantly to the emerging post-antibiotic era. | 2016 | 27482806 |
| highly differentiated human airway epithelial cells: a model to study host cell-parasite interactions in pertussis. | bordetella pertussis colonizes the human respiratory mucosa. most studies on b. pertussis adherence have relied on cultured mammalian cells that lack key features present in differentiated human airway cells or on animal models that are not natural hosts of b. pertussis. the objectives of this work were to evaluate b. pertussis infection in highly differentiated human airway cells in vitro and to show the role of b. pertussis fimbriae in cell adherence. | 2016 | 26492208 |
| plasmacytoid dendritic cell-derived ifnα modulates th17 differentiation during early bordetella pertussis infection in mice. | whooping cough is a highly contagious respiratory disease caused by bordetella pertussis (b. pertussis). t helper 17 (th17) cells have a central role in the resolution of the infection. emerging studies document that type i interferons (ifns) suppress th17 differentiation and interleukin (il)-17 responses in models of infection and chronic inflammation. as plasmacytoid dendritic cells (pdcs) are a major source of type i ifns, we hypothesize that during b. pertussis infection in mice, pdc-derived ... | 2016 | 26462419 |
| validation of a loop-mediated isothermal amplification assay for rapid diagnosis of pertussis infection in nasopharyngeal samples. | to develop and validate a novel loop-mediated amplification (lamp) assay for rapid diagnosis (<1 hour) of whooping cough in nasopharyngeal samples versus the gold standard: real-time pcr. | 2016 | 26565672 |
| pertussis vaccines: who position paper, august 2015--recommendations. | this article presents the world health organization's (who) recommendations for the use of vaccines against bordetella pertussis from the who position paper on pertussis vaccines: who position paper--august 2015, recently published in the weekly epidemiological record (pertussis vaccines: who position paper. wkly epidemiol rec 2015;90(august(35)):433-60). this position paper summarizes the most recent developments in the field of pertussis disease and its prevention by vaccination. it includes t ... | 2016 | 26562318 |
| absence of bordetella pertussis among infants hospitalized for bronchiolitis in finland, 2008-2010. | in 169 finnish infants hospitalized for bronchiolitis at age <6 months in 2008-2010, nasopharyngeal aspirates were tested by polymerase chain reaction for bordetella pertussis and 16 viruses. respiratory viruses were detected in 89% (71% with respiratory syncytial virus), but no infant had b. pertussis. the latter finding may reflect a positive effect from the broadening of the finnish pertussis vaccination program in 2005. | 2016 | 26551447 |
| the potential role of subclinical bordetella pertussis colonization in the etiology of multiple sclerosis. | it is established that (1) subclinical bordetella pertussis colonization of the nasopharynx persists in highly vaccinated populations, and (2) b. pertussis toxin is a potent adjuvant that, when co-administered with neural antigens, induces neuropathology in experimental autoimmune encephalomyelitis, the principle animal model of multiple sclerosis. building on these observations with supporting epidemiologic and biologic evidence, we propose that, contrary to conventional wisdom that subclinical ... | 2016 | 26724970 |
| bordetella pertussis filamentous hemagglutinin itself does not trigger anti-inflammatory interleukin-10 production by human dendritic cells. | filamentous hemagglutinin (fha) is an important adhesin of the whooping cough agent bordetella pertussis and is contained in most acellular pertussis vaccines. recently, fha was proposed to exert an immunomodulatory activity through induction of tolerogenic il-10 secretion from dendritic cells. we have re-evaluated the cytokine-inducing activity of fha, placing specific emphasis on the role of the residual endotoxin contamination of fha preparations. we show that endotoxin depletion did not affe ... | 2016 | 26699834 |
| the relationship between bordetella pertussis genotype and clinical severity in australian children with pertussis. | changes in circulating bordetella pertussis genotypes, including a novel pertussis toxin promoter ptxp3 allele and absence of pertactin (prn) antigen, have been reported from several countries but limited data on relative severity are available. we compared markers of disease severity in children with b. pertussis infection due to strains of differing genotype. | 2016 | 26675318 |
| adenylate cyclase toxin-mediated delivery of the s1 subunit of pertussis toxin into mammalian cells. | the adenylate cyclase toxin (act) of bordetella pertussis internalizes its catalytic domain into target cells. act can function as a tool for delivering foreign protein antigen moieties into immune effector cells to induce a cytotoxic t lymphocyte response. in this study, we replaced the catalytic domain of act with an enzymatically active protein moiety, the s1 (adp-ribosyltransferase) subunit of pertussis toxin (pt). the s1 moiety was successfully internalized independent of endocytosis into s ... | 2016 | 26607401 |
| loss of nrf2 exacerbates the visual deficits and optic neuritis elicited by experimental autoimmune encephalomyelitis. | optic neuritis, inflammation of the optic nerve, is experienced by most patients with multiple sclerosis (ms) and is typically characterized by episodes of acute, monocular vision loss. these episodes of inflammation can lead to damage or degeneration of the retinal ganglion cells (rgcs), the axons of which comprise the optic nerve. experimental autoimmune encephalomyelitis (eae) is a well-established model of ms in which mice are immunized to produce a neuroautoimmunity that recapitulates the c ... | 2016 | 28050123 |
| mucosal vaccine development based on liposome technology. | immune protection against infectious diseases is most effective if located at the portal of entry of the pathogen. hence, there is an increasing demand for vaccine formulations that can induce strong protective immunity following oral, respiratory, or genital tract administration. at present, only few mucosal vaccines are found on the market, but recent technological advancements and a better understanding of the principles that govern priming of mucosal immune responses have contributed to a mo ... | 2016 | 28127567 |
| minimizing proteome redundancy in the uniprot knowledgebase. | advances in high-throughput sequencing have led to an unprecedented growth in genome sequences being submitted to biological databases. in particular, the sequencing of large numbers of nearly identical bacterial genomes during infection outbreaks and for other large-scale studies has resulted in a high level of redundancy in nucleotide databases and consequently in the uniprot knowledgebase (uniprotkb). redundancy negatively impacts on database searches by causing slower searches, an increase i ... | 2016 | 28025334 |
| identification of dietzia spp. from cardiac tissue by 16s rrna pcr in a patient with culture-negative device-associated endocarditis: a case report and review of the literature. | the genus dietzia was recently distinguished from other actinomycetes such as rhodococcus. while these organisms are known to be distributed widely in the environment, over the past decade several novel species have been described and isolated from human clinical specimens. here we describe the identification of dietzia natronolimnaea/d. cercidiphylli by pcr amplification and sequencing of the 16s rrna encoding gene from cardiac tissue in a patient with culture-negative device-associated endocar ... | 2016 | 28101387 |
| complete genome sequences of four bordetella pertussis vaccine reference strains from serum institute of india. | serum institute of india is among the world's largest vaccine producers. here, we report the complete genome sequences for four bordetella pertussis strains used by serum institute of india in the production of whole-cell pertussis vaccines. | 2016 | 28007855 |
| impact and influence of the natural vibrio-squid symbiosis in understanding bacterial-animal interactions. | animals are colonized by bacteria, and in many cases partners have co-evolved to perform mutually beneficial functions. an exciting and ongoing legacy of the past decade has been an expansion of technology to enable study of natural associations in situ/in vivo. as a result, more symbioses are being examined, and additional details are being revealed for well-studied systems with a focus on the interactions between partners in the native context. with this framing, we review recent literature fr ... | 2016 | 28018314 |
| interaction with adenylate cyclase toxin from bordetella pertussis affects the metal binding properties of calmodulin. | adenylate cyclase toxin domain (cyaa-acd) is a calmodulin (cam)-dependent adenylate cyclase involved in bordetella pertussis pathogenesis. calcium (ca(2+)) and magnesium (mg(2+)) concentrations impact cam-dependent cyaa-acd activation, but the structural mechanisms remain unclear. in this study, nmr, dynamic light scattering, and native page were used to probe mg(2+)-induced transitions in cam's conformation in the presence of cyaa-acd. mg(2+) binding was localized to sites i and ii, while sites ... | 2016 | 28097085 |
| interaction with adenylate cyclase toxin from bordetella pertussis affects the metal binding properties of calmodulin. | adenylate cyclase toxin domain (cyaa-acd) is a calmodulin (cam)-dependent adenylate cyclase involved in bordetella pertussis pathogenesis. calcium (ca(2+)) and magnesium (mg(2+)) concentrations impact cam-dependent cyaa-acd activation, but the structural mechanisms remain unclear. in this study, nmr, dynamic light scattering, and native page were used to probe mg(2+)-induced transitions in cam's conformation in the presence of cyaa-acd. mg(2+) binding was localized to sites i and ii, while sites ... | 2016 | 28097085 |
| tolerance checkpoint bypass permits emergence of pathogenic t cells to neuromyelitis optica autoantigen aquaporin-4. | aquaporin-4 (aqp4)-specific t cells are expanded in neuromyelitis optica (nmo) patients and exhibit th17 polarization. however, their pathogenic role in cns autoimmune inflammatory disease is unclear. although multiple aqp4 t-cell epitopes have been identified in wt c57bl/6 mice, we observed that neither immunization with those determinants nor transfer of donor t cells targeting them caused cns autoimmune disease in recipient mice. in contrast, robust proliferation was observed following immuni ... | 2016 | 27940915 |
| actin activates pseudomonas aeruginosa exoy nucleotidyl cyclase toxin and exoy-like effector domains from martx toxins. | the nucleotidyl cyclase toxin exoy is one of the virulence factors injected by the pseudomonas aeruginosa type iii secretion system into host cells. inside cells, it is activated by an unknown eukaryotic cofactor to synthesize various cyclic nucleotide monophosphates. exoy-like adenylate cyclases are also found in multifunctional-autoprocessing repeats-in-toxin (martx) toxins produced by various gram-negative pathogens. here we demonstrate that filamentous actin (f-actin) is the hitherto unknown ... | 2016 | 27917880 |
| seroprevalence of bordetella pertussis specific immunoglobulin g antibody levels among asymptomatic individuals aged 4 to 24 years: a descriptive cross sectional study from sri lanka. | in sri lanka pertussis continues to circulate in the community and cases among adolescents and adults have been reported despite 95% coverage of the four dose pertussis vaccination during early childhood. waning of immunity following natural infection or immunization may contribute to the persistent circulation. an adolescent booster dose is not included in the national immunization schedule of sri lanka, although this is routine practice in many countries. therefore information on immunity to p ... | 2016 | 27905894 |
| crystal structure of chitinase chiw from paenibacillus sp. str. fpu-7 reveals a novel type of bacterial cell-surface-expressed multi-modular enzyme machinery. | the gram-positive bacterium paenibacillus sp. str. fpu-7 effectively hydrolyzes chitin by using a number of chitinases. a unique chitinase with two catalytic domains, chiw, is expressed on the cell surface of this bacterium and has high activity towards various chitins, even crystalline chitin. here, the crystal structure of chiw at 2.1 å resolution is presented and describes how the enzyme degrades chitin on the bacterial cell surface. the crystal structure revealed a unique multi-modular archi ... | 2016 | 27907169 |
| bordetella pertussis outer membrane vesicle vaccine confers equal efficacy in mice with milder inflammatory responses compared to a whole-cell vaccine. | the demand for improved pertussis vaccines is urgent due to the resurgence of whooping cough. a deeper understanding of the mode of action of pertussis vaccines is required to achieve this improvement. the vaccine-induced effects of a candidate outer membrane vesicle vaccine (omvpv) and a classical protective but reactogenic whole cell vaccine (wpv) were comprehensively compared in mice. the comparison revealed essential qualitative and quantitative differences with respect to immunogenicity and ... | 2016 | 27905535 |
| cysteine-mediated gene expression and characterization of the cmbr regulon in streptococcus pneumoniae. | in this study, we investigated the transcriptomic response of streptococcus pneumoniae d39 to cysteine. transcriptome comparison of the d39 wild-type grown at a restricted concentration of cysteine (0.03 mm) to one grown at a high concentration of cysteine (50 mm) in chemically-defined medium (cdm) revealed elevated expression of various genes/operons, i.e., spd-0150, metq, spd-0431, metef, gsht, spd-0618, fhs, tcyb, metb-csd, meta, spd-1898, yvde, and cysk, likely to be involved in the transpor ... | 2016 | 27990139 |
| fulminant ecchymosis as the initial manifestation of antiphospholipid syndrome (aps) triggered by respiratory syncytial virus (rsv) infection: a case report and review of the literature. | we present a unique and informative instance of respiratory syncytial virus (rsv) infection associated with antiphospholipid syndrome (aps), and discuss this case in the context of the literature addressing the immunopathogenesis of aps associated with diverse infections. we describe the case of a 43-year-old man with no significant past medical history who presented with the acute onset of fever, hemoptysis, and extensive bullous, ecchymotic lesions in both lower extremities. punch biopsy of th ... | 2016 | 27920986 |
| fulminant ecchymosis as the initial manifestation of antiphospholipid syndrome (aps) triggered by respiratory syncytial virus (rsv) infection: a case report and review of the literature. | we present a unique and informative instance of respiratory syncytial virus (rsv) infection associated with antiphospholipid syndrome (aps), and discuss this case in the context of the literature addressing the immunopathogenesis of aps associated with diverse infections. we describe the case of a 43-year-old man with no significant past medical history who presented with the acute onset of fever, hemoptysis, and extensive bullous, ecchymotic lesions in both lower extremities. punch biopsy of th ... | 2016 | 27920986 |
| a parasite-derived 68-mer peptide ameliorates autoimmune disease in murine models of type 1 diabetes and multiple sclerosis. | helminth parasites secrete molecules that potently modulate the immune responses of their hosts and, therefore, have potential for the treatment of immune-mediated human diseases. fhhdm-1, a 68-mer peptide secreted by the helminth parasite fasciola hepatica, ameliorated disease in two different murine models of autoimmunity, type 1 diabetes and relapsing-remitting immune-mediated demyelination. unexpectedly, fhhdm-1 treatment did not affect the proliferation of auto-antigen specific t cells or t ... | 2016 | 27883079 |
| epidemiology of pertussis in two ibero-american countries with different vaccination policies: lessons derived from different surveillance systems. | pertussis is a re-emerging disease worldwide despite its high vaccination coverage. european and latin-american countries have used different surveillance and vaccination policies against pertussis. we compared the epidemiology of this disease in two ibero-american countries with different vaccination and surveillance policies. | 2016 | 27876021 |
| an anti-tnf-α antibody mimetic to treat ocular inflammation. | infliximab is an antibody that neutralizes tnf-α and is used principally by systemic administration to treat many inflammatory disorders. we prepared the antibody mimetic fab-peg-fab (fpfinfliximab) for direct intravitreal injection to assess whether such formulations have biological activity and potential utility for ocular use. fpfinfliximab was designed to address side effects caused by antibody degradation and the presence of the fc region. surface plasmon resonance analysis indicated that i ... | 2016 | 27874029 |
| in vitro activity of solithromycin against bordetella pertussis, an emerging respiratory pathogen. | there has been an increase in the number of pertussis cases reported since the introduction of the acellular pertussis vaccine. while children that present with pertussis have a characteristic whooping cough, adults can simply have a persistent, nonspecific cough and remain undiagnosed. macrolide antibiotics, such as azithromycin, are the currently recommended treatment for pertussis. solithromycin is a new macrolide and the first fluoroketolide with broad activity against a wide spectrum of bac ... | 2016 | 27620481 |
| concentrations of immunoglobulin g antibodies against pertussis toxin does not decrease over a long period of time in japan. | objective adult patients with pertussis rarely show typical symptoms, such as paroxysmal coughing, inspiratory "whoop", or post-tussive vomiting. while a culture is regarded as the gold standard for diagnosis, the sensitivity is very low. therefore, the diagnosis of pertussis in adults in clinical practice is mostly based on single-sample serology using an enzyme-linked immunosorbent assay (elisa) with the pertussis toxin antigen. various cut-off values for the anti-pertussis toxin igg (pt-igg) ... | 2016 | 27853066 |
| factors influencing the spread of pertussis in households: a prospective study, catalonia and navarre, spain, 2012 to 2013. | we aimed to investigate transmission rates of pertussis in household contacts of cases and factors associated with transmission. a prospective epidemiological study was conducted in 2012 and 2013 to determine the incidence of pertussis among household contacts of reported cases in catalonia and navarre, spain. an epidemiological survey was completed for each case and contact, who were followed for 28 days to determine the source of infection (primary case) and detect the occurrence of secondary ... | 2016 | 27918260 |
| gm-csf-producing th cells in rats sensitive and resistant to experimental autoimmune encephalomyelitis. | given that granulocyte macrophage colony-stimulating factor (gm-csf) is identified as the key factor to endow auto-reactive th cells with the potential to induce neuroinflammation in experimental autoimmune encephalomyelitis (eae) models, the frequency and phenotype of gm-csf-producing (gm-csf+) th cells in draining lymph nodes (dlns) and spinal cord (sc) of albino oxford (ao) and dark agouti (da) rats immunized for eae were examined. the generation of neuroantigen-specific gm-csf+ th lymphocyte ... | 2016 | 27832210 |
| perspectives for therapeutic hpv vaccine development. | human papillomavirus (hpv) infections and associated diseases remain a serious burden worldwide. it is now clear that hpv serves as the etiological factor and biologic carcinogen for hpv-associated lesions and cancers. although preventative hpv vaccines are available, these vaccines do not induce strong therapeutic effects against established hpv infections and lesions. these concerns create a critical need for the development of therapeutic strategies, such as vaccines, to treat these existing ... | 2016 | 27809842 |
| draft genome sequence of a novel luteimonas sp. strain from coral mucus, hawai'i. | luteimonas sp. strain jm171 was cultivated from mucus collected around the coral porites lobata the jm171 draft genome of 2,992,353 bp contains 2,672 protein-coding open reading frames, 45 trna coding regions, and encodes a putative globin-coupled diguanylate cyclase, jmgreg. | 2016 | 27811107 |
| identification of a two-component class iib bacteriocin in streptococcus pyogenes by recombinase-based in vivo expression technology. | streptococcus pyogenes is a globally prominent bacterial pathogen that exhibits strict tropism for the human host, yet bacterial factors responsible for the ability of s. pyogenes to compete within this limited biological niche are not well understood. using an engineered recombinase-based in vivo expression technology (rivet) system, we identified an in vivo-induced promoter region upstream of a predicted class iib bacteriocin system in the m18 serotype s. pyogenes strain mgas8232. this promote ... | 2016 | 27808235 |
| epidemiology of pertussis among young pakistani infants: a community-based prospective surveillance study. | pertussis remains a cause of morbidity and mortality among young infants. there are limited data on the pertussis disease burden in this age group from low- and lower-middle-income countries, including in south asia. | 2016 | 27838667 |
| forecasting epidemiological consequences of maternal immunization. | the increase in the incidence of whooping cough (pertussis) in many countries with high vaccination coverage is alarming. maternal pertussis immunization has been proposed as an effective means of protecting newborns during the interval between birth and the first routine dose. however, there are concerns regarding potential interference between maternal antibodies and the immune response elicited by the routine schedule, with possible long-term population-level effects. | 2016 | 27838674 |
| pertussis in young infants throughout the world. | in typical pertussis in young infants, the child will appear deceptively well; he or she will have coryza, sneezing, and a mild cough. there is no fever. this progresses to gagging, gasping, eye bulging, bradycardia, cyanosis, and vomiting. there is leukocytosis with lymphocytosis and apneic episodes. deaths relate to leukocytosis, pulmonary hypertension, and pneumonia. the source of pertussis in young infants is most often a family member with cough illness that is not recognized as pertussis. ... | 2016 | 27838663 |
| pertussis-associated pneumonia in infants and children from low- and middle-income countries participating in the perch study. | few data exist describing pertussis epidemiology among infants and children in low- and middle-income countries to guide preventive strategies. | 2016 | 27838672 |
| the burden of pertussis hospitalization in hiv-exposed and hiv-unexposed south african infants. | there are limited data on pertussis in african children, including among human immunodeficiency virus (hiv)-exposed infants. we conducted population-based hospital surveillance to determine the incidence and clinical presentation of bordetella pertussis-associated hospitalization in perinatal hiv-exposed and -unexposed infants. | 2016 | 27838669 |
| assessing the evidence for maternal pertussis immunization: a report from the bill & melinda gates foundation symposium on pertussis infant disease burden in low- and lower-middle-income countries. | implementation of effective interventions has halved maternal and child mortality over the past 2 decades, but less progress has been made in reducing neonatal mortality. almost 45% of under-5 global mortality now occurs in infants <1 month of age, with approximately 86% of neonatal deaths occurring in low- and lower-middle-income countries (lmics). as an estimated 23% of neonatal deaths globally are due to infectious causes, maternal immunization (mi) is one intervention that may reduce mortali ... | 2016 | 27838664 |
| global childhood deaths from pertussis: a historical review. | impact of pertussis vaccines on mortality is a key world health organization indicator, and trends in mortality rates and age distribution can inform maternal immunization strategies. we systematically reviewed studies reporting pertussis mortality rates (pmrs) per million population, identifying 19 eligible studies. during a prevaccine observation period of ≥50 years in high-income countries (hics), pmrs reduced in both infants and 1- to 4-year-olds by >80%, along with improvements in living co ... | 2016 | 27838665 |
| bordetella pertussis infection in south african hiv-infected and hiv-uninfected mother-infant dyads: a longitudinal cohort study. | there is a paucity of data regarding the burden of bordetella pertussis in african women and young infants, and particularly the impact of maternal human immunodeficiency virus (hiv) infection thereon. we performed a retrospective analysis of respiratory illness samples from longitudinal cohorts of hiv-uninfected and hiv-infected women and their infants to evaluate the burden of pertussis illness in a black-african community. | 2016 | 27838670 |
| the importance of persistence. | 2016 | 27838662 | |
| influence of population demography and immunization history on the impact of an antenatal pertussis program. | antenatal pertussis vaccination is being considered as a means to reduce the burden of infant pertussis in low- and middle-income countries (lmics), but its likely impact in such settings is yet to be quantified. | 2016 | 27838675 |
| pertussis incidence by time, province and age group in iran, 2006-2011. | 2016 | 28028509 | |
| chronic cough in korean adults: a literature review on common comorbidity. | chronic cough is a significant medical condition with high prevalence and a strong negative impact on the quality of life. cough hypersensitivity is thought to underlie chronic cough, with several environmental and host factors interacting to cause neuronal sensitization and chronicity. comorbid conditions affecting cough reflex pathways, such as upper airway diseases, asthma, and gastroesophageal reflux, play important roles in chronic cough. however, their prevalence may vary in patients livin ... | 2016 | 27803879 |
| noninvasive recognition and biomarkers of early allergic asthma in cats using multivariate statistical analysis of nmr spectra of exhaled breath condensate. | asthma is prevalent in children and cats, and needs means of noninvasive diagnosis. we sought to distinguish noninvasively the differences in 53 cats before and soon after induction of allergic asthma, using nmr spectra of exhaled breath condensate (ebc). statistical pattern recognition was improved considerably by preprocessing the spectra with probabilistic quotient normalization and glog transformation. classification of the 106 preprocessed spectra by principal component analysis and partial ... | 2016 | 27764146 |
| specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone. | there are various autoimmunogenic antigens (ais) in testicular germ cells (tgcs) recognized as foreign by the body's immune system. however, there is little information of tgc-specific ais being available. the aim of this study is to identify tgc-specific ais. we have previously established that immunization using viable syngeneic tgc can also induce murine experimental autoimmune orchitis (eao) without using any adjuvant. this study is to identify tgc-specific ais by tgc liquid chromatography-t ... | 2016 | 27752123 |
| hospital admissions for lower respiratory tract infections among infants in the canadian arctic: a cohort study. | it is unknown whether this burden of disease of lower respiratory tract infections is comparable across the canadian arctic. the objectives of this surveillance study were to compare the rates of hospital admission for lower respiratory tract infection and the severity of infection across arctic canada, and to describe the responsible viruses. | 2016 | 28018874 |
| pertussis toxin exploits host cell signaling pathways induced by meningitis-causing e. coli k1-rs218 and enhances adherence of monocytic thp-1 cells to human cerebral endothelial cells. | pertussis toxin (ptx), the major virulence factor of the whooping cough-causing bacterial pathogen bordetella pertussis, permeabilizes the blood-brain barrier (bbb) in vitro and in vivo. breaking barriers might promote translocation of meningitis-causing bacteria across the bbb, thereby facilitating infection. ptx activates several host cell signaling pathways exploited by the neonatal meningitis-causing escherichia coli k1-rs218 for invasion and translocation across the bbb. here, we investigat ... | 2016 | 27754355 |
| non-specific immunological effects of selected routine childhood immunisations: systematic review. | to identify and characterise non-specific immunological effects after routine childhood vaccines against bcg, measles, diphtheria, pertussis, and tetanus. | 2016 | 27737830 |
| induction of gut regulatory cd39(+) t cells by teriflunomide protects against eae. | to determine whether as an orally delivered treatment, teriflunomide, an inhibitor of the mitochondrial enzyme dihydroorotate dehydrogenase approved to treat relapsing forms of multiple sclerosis, could affect gut-associated lymphoid tissue (galt) immune responses functionally. | 2016 | 27766282 |
| complete genome sequences of four different bordetella sp. isolates causing human respiratory infections. | species of the genus bordetella associate with various animal hosts, frequently causing respiratory disease. bordetella pertussis is the primary agent of whooping cough and other bordetella species can cause similar cough illness. here, we report four complete genome sequences from isolates of different bordetella species recovered from human respiratory infections. | 2016 | 27795250 |
| immunological signatures after bordetella pertussis infection demonstrate importance of pulmonary innate immune cells. | effective immunity against bordetella pertussis is currently under discussion following the stacking evidence of pertussis resurgence in the vaccinated population. natural immunity is more effective than vaccine-induced immunity indicating that knowledge on infection-induced responses may contribute to improve vaccination strategies. we applied a systems biology approach comprising microarray, flow cytometry and multiplex immunoassays to unravel the molecular and cellular signatures in unprotect ... | 2016 | 27711188 |
| b cell-derived transforming growth factor-β1 expression limits the induction phase of autoimmune neuroinflammation. | studies in experimental autoimmune encephalomyelitis (eae), a murine model of multiple sclerosis (ms), have shown that regulatory b cells modulate the course of the disease via the production of suppressive cytokines. while data indicate a role for transforming growth factor (tgf)-β1 expression in regulatory b cell functions, this mechanism has not yet been tested in autoimmune neuroinflammation. transgenic mice deficient for tgf-β1 expression in b cells (b-tgf-β1(-/-)) were tested in eae induce ... | 2016 | 27708418 |
| moringin activates wnt canonical pathway by inhibiting gsk3β in a mouse model of experimental autoimmune encephalomyelitis. | aberrant canonical wnt-β-catenin signaling has been reported in multiple sclerosis (ms), although the results are controversial. the present study aimed to examine the role of the wnt-β-catenin pathway in experimental ms and also to test moringin (4-[α-l-rhamnopyranosyloxy]-benzyl isothiocyanate), resulting from exogenous myrosinase hydrolysis of the natural phytochemical glucomoringin 4(α-l-rhamnosyloxy)-benzyl glucosinolate as a modulator of neuroinflammation via the β-catenin-pparγ axis. expe ... | 2016 | 27784989 |
| structure and specificity of the type vi secretion system clpv-tssc interaction in enteroaggregative escherichia coli. | the type vi secretion system (t6ss) is a versatile machine that delivers toxins into either eukaryotic or bacterial cells. it thus represents a key player in bacterial pathogenesis and inter-bacterial competition. schematically, the t6ss can be viewed as a contractile tail structure anchored to the cell envelope. the contraction of the tail sheath propels the inner tube loaded with effectors towards the target cell. the components of the contracted tail sheath are then recycled by the clpv aaa(+ ... | 2016 | 27698444 |
| consensus recommendation for india and bangladesh for the use of pneumococcal vaccine in mass gatherings with special reference to hajj pilgrims. | respiratory tract infections are prevalent among hajj pilgrims with pneumonia being a leading cause of hospitalization. streptococcus pneumoniae is a common pathogen isolated from patients with pneumonia and respiratory tract infections during hajj. there is a significant burden of pneumococcal disease in india, which can be prevented. guidelines for preventive measures and adult immunization have been published in india, but the implementation of the guidelines is low. data from bangladesh are ... | 2016 | 27942192 |
| the impact of healthcare visit timing on reported pertussis cough duration: selection bias and disease pattern from reported cases in michigan, usa, 2000-2010. | pertussis is a potentially serious respiratory illness characterized by cough of exceptionally long duration of up to approximately100 days. while macrolide antibiotics are an effective treatment, there is an ongoing debate whether they also shorten the length of cough symptoms. we investigated whether public health surveillance data for pertussis, in which cases are identified at diagnosis, are potentially affected by selection bias and the possible consequences for reported cough duration. | 2016 | 27682251 |
| effectiveness of pertussis vaccination and duration of immunity. | a resurgence of pertussis cases among both vaccinated and unvaccinated people raises questions about vaccine effectiveness over time. our objective was to study the effectiveness of the pertussis vaccine and characterize the effect of waning immunity and whole-cell vaccine priming. | 2016 | 27672225 |
| apparent life-threatening event in infancy. | an apparent life-threatening event (alte) is defined as the combination of clinical presentations such as apnea, marked change in skin and muscle tone, gagging, or choking. it is a frightening event, and it predominantly occurs during infancy at a mean age of 1-3 months. the causes of alte are categorized into problems that are: gastrointestinal (50%), neurological (30%), respiratory (20%), cardiovascular (5%), metabolic and endocrine (2%-5%), or others such as child abuse. up to 50% of altes ar ... | 2016 | 27721838 |
| a new socioeconomic status measure for vaccine research in children using individual housing data: a population-based case-control study. | we recently developed houses, an individual housing-based socioeconomic status (ses) measurement for health disparities research. we assessed whether houses was associated with risk of pertussis and pertussis vaccine up-to-date status in children. | 2016 | 27655468 |
| complete genome sequences of bordetella pertussis vaccine reference strains 134 and 10536. | vaccine formulations and vaccination programs against whooping cough (pertussis) vary worldwide. here, we report the complete genome sequences of two divergent bordetella pertussis reference strains used in the production of pertussis vaccines. | 2016 | 27635001 |
| complete genome sequence of bordetella pertussis strain va-190 isolated from a vaccinated 10-year-old patient with whooping cough. | the number of cases of pertussis has increased in the united states despite vaccination. we present the genome of an isolate of bordetella pertussis from a vaccinated patient from virginia. the genome was sequenced by long-read methodology and compared to that of a clinical isolate used for laboratory studies, d420. | 2016 | 27634997 |
| a novel high-throughput assay to quantify the vaccine-induced inhibition of bordetella pertussis adhesion to airway epithelia. | pertussis or whooping cough is an acute respiratory illness caused by the gram-negative pathogen bordetella pertussis. despite high vaccination coverage whooping cough is currently re-emerging in many developed countries. although the causes of pertussis resurgence are matter of debate, emerging evidences suggest that acellular vaccines efficiently protect against the hallmark symptoms of pertussis disease but fail to prevent colonization. this presumably impacts on increased risk of bacterial t ... | 2016 | 27633511 |
| degp chaperone suppresses toxic inner membrane translocation intermediates. | the periplasm of gram-negative bacteria includes a variety of molecular chaperones that shepherd the folding and targeting of secreted proteins. a central player of this quality control network is degp, a protease also suggested to have a chaperone function. we serendipitously discovered that production of the bordetella pertussis autotransporter virulence protein pertactin is lethal in escherichia coli δdegp strains. we investigated specific contributions of degp to secretion of pertactin as a ... | 2016 | 27626276 |
| mitochondrial dysfunction is an important cause of neurological deficits in an inflammatory model of multiple sclerosis. | neuroinflammation can cause major neurological dysfunction, without demyelination, in both multiple sclerosis (ms) and a mouse model of the disease (experimental autoimmune encephalomyelitis; eae), but the mechanisms remain obscure. confocal in vivo imaging of the mouse eae spinal cord reveals that impaired neurological function correlates with the depolarisation of both the axonal mitochondria and the axons themselves. indeed, the depolarisation parallels the expression of neurological deficit ... | 2016 | 27624721 |
| in vivo quantification of the secretion rates of the hemolysin a type i secretion system. | type 1 secretion systems (t1ss) of gram-negative bacteria secrete a broad range of substrates into the extracellular space. common to all substrates is a c-terminal secretion sequence and nonapeptide repeats in the c-terminal part that bind ca(2+) in the extracellular space, to trigger protein folding. like all t1ss, the hemolysin a (hlya) t1ss of escherichia coli consists of an abc transporter, a membrane fusion protein and an outer membrane protein allowing the one step translocation of the su ... | 2016 | 27616645 |
| epidemiology of pertussis in denmark, 1995 to 2013. | we describe incidence and age distribution of laboratory-confirmed pertussis in denmark from 1995 to 2013. notification has been mandatory since 2007. since 1997, an acellular monocomponent vaccine has been used. the latest epidemic occurred in 2002 with an incidence of 36 per 100,000; since 1995, only six infant deaths have been recorded. the inter-epidemic incidence lies below 10 per 100,000. in 1995, the mean age of confirmed cases was 9.2 years (95% confidence interval (ci): 7.9-10.5; median ... | 2016 | 27632433 |
| skin barrier recovery by protease-activated receptor-2 antagonist lobaric acid. | atopic dermatitis (ad) results from gene and environment interactions that lead to a range of immunological abnormalities and breakdown of the skin barrier. protease-activated receptor 2 (par2) belongs to a family of g-protein coupled receptors and is expressed in suprabasal layers of the epidermis. par2 is activated by both trypsin and a specific agonist peptide, sligkv-nh₂ and is involved in both epidermal permeability barrier homeostasis and epithelial inflammation. in this study, we investig ... | 2016 | 27169822 |
| a cough-based algorithm for automatic diagnosis of pertussis. | pertussis is a contagious respiratory disease which mainly affects young children and can be fatal if left untreated. the world health organization estimates 16 million pertussis cases annually worldwide resulting in over 200,000 deaths. it is prevalent mainly in developing countries where it is difficult to diagnose due to the lack of healthcare facilities and medical professionals. hence, a low-cost, quick and easily accessible solution is needed to provide pertussis diagnosis in such areas to ... | 2016 | 27583523 |
| negatively charged residues of the segment linking the enzyme and cytolysin moieties restrict the membrane-permeabilizing capacity of adenylate cyclase toxin. | the whooping cough agent, bordetella pertussis, secretes an adenylate cyclase toxin-hemolysin (cyaa) that plays a crucial role in host respiratory tract colonization. cyaa targets cr3-expressing cells and disrupts their bactericidal functions by delivering into their cytosol an adenylate cyclase enzyme that converts intracellular atp to camp. in parallel, the hydrophobic domain of cyaa forms cation-selective pores that permeabilize cell membrane. the invasive ac and pore-forming domains of cyaa ... | 2016 | 27581058 |
| time series analysis of temporal trends in the pertussis incidence in mainland china from 2005 to 2016. | short-term forecast of pertussis incidence is helpful for advanced warning and planning resource needs for future epidemics. by utilizing the auto-regressive integrated moving average (arima) model and exponential smoothing (ets) model as alterative models with r software, this paper analyzed data from chinese center for disease control and prevention (china cdc) between january 2005 and june 2016. the arima (0,1,0)(1,1,1)12 model (aicc = 1342.2 bic = 1350.3) was selected as the best performing ... | 2016 | 27577101 |
| the regulator of g-protein signaling rgs16 promotes insulin secretion and β-cell proliferation in rodent and human islets. | g protein-coupled receptor (gpcr) signaling regulates insulin secretion and pancreatic β cell-proliferation. while much knowledge has been gained regarding how gpcrs are activated in β cells, less is known about the mechanisms controlling their deactivation. in many cell types, termination of gpcr signaling is controlled by the family of regulators of g-protein signaling (rgs). rgs proteins are expressed in most eukaryotic cells and ensure a timely return to the gpcr inactive state upon removal ... | 2016 | 27689011 |
| vaccine-mediated activation of human tlr4 is affected by modulation of culture conditions during whole-cell pertussis vaccine preparation. | the potency of whole-cell pertussis (wp) vaccines is still determined by an intracerebral mouse protection test. to allow development of suitable in vitro alternatives to this test, insight into relevant parameters to monitor the consistency of vaccine quality is essential. to this end, a panel of experimental wp vaccines of varying quality was prepared by sulfate-mediated suppression of the bvgasr master virulence regulatory system of bordetella pertussis during cultivation. this system regulat ... | 2016 | 27548265 |
| linking susceptibility to infectious diseases to immune system abnormalities among hiv-exposed uninfected infants. | hiv-exposed uninfected (heu) infants experience increased overall mortality from infectious causes when compared to hiv-unexposed uninfected (hu) infants. this is the case in both the resource-rich and resource-limited settings. here, we explore the concept that specific types of infectious diseases that are more common among heu infants could provide clues as to the potential underlying immunological abnormalities. the most commonly reported infections in heu vs. hu infants are caused by encaps ... | 2016 | 27594857 |
| a simple luminescent adenylate-cyclase functional assay for evaluation of bacillus anthracis edema factor activity. | edema factor (ef), the toxic sub-unit of the bacillus anthracis edema toxin (et) is a calmodulin-dependent adenylate cyclase whose detrimental activity in the infected host results in severe edema. ef is therefore a major virulence factor of b. anthracis. we describe a simple, rapid and reliable functional adenylate-cyclase assay based on inhibition of a luciferase-mediated luminescence reaction. the assay exploits the efficient adenylate cyclase-mediated depletion of adenosine tri-phosphate (at ... | 2016 | 27548219 |
| evaluation of outbreak response immunization in the control of pertussis using agent-based modeling. | pertussis control remains a challenge due to recently observed effects of waning immunity to acellular vaccine and suboptimal vaccine coverage. multiple outbreaks have been reported in different ages worldwide. for certain outbreaks, public health authorities can launch an outbreak response immunization (ori) campaign to control pertussis spread. we investigated effects of an outbreak response immunization targeting young adolescents in averting pertussis cases. | 2016 | 27602299 |
| increase in pertussis cases along with high prevalence of two emerging genotypes of bordetella pertussis in perú, 2012. | as has occurred in many regions worldwide, in 2012 the incidence of pertussis increased in perú. this epidemiologic situation has been associated with a waning vaccine-induced immunity and the adaptation of bordetella pertussis to vaccine-induced immunity along with improved diagnostic methods. | 2016 | 27530444 |
| respiratory presentation of pediatric patients in the 2014 enterovirus d68 outbreak. | background. in the fall of 2014, a north american outbreak of enterovirus d68 resulted in a significant number of pediatric hospital admissions for respiratory illness throughout north america. this study characterized the clinical presentation and risk factors for a severe clinical course in children admitted to british columbia children's hospital during the 2014 outbreak. methods. retrospective chart review of patients with confirmed ev-d68 infection admitted to bcch with respiratory symptoms ... | 2016 | 27610028 |
| capture-recapture estimators in epidemiology with applications to pertussis and pneumococcal invasive disease surveillance. | surveillance networks are often not exhaustive nor completely complementary. in such situations, capture-recapture methods can be used for incidence estimation. the choice of estimator and their robustness with respect to the homogeneity and independence assumptions are however not well documented. | 2016 | 27529167 |
| lncrnas bcyrn1 promoted the proliferation and migration of rat airway smooth muscle cells in asthma via upregulating the expression of transient receptor potential 1. | long noncoding rnas (lncrnas) played important roles in several biological processes through regulating the expression of protein. however, the function of lncrna bcyrn1 in airway smooth muscle cells (asmcs) has not been reported. | 2016 | 27648131 |
| pertussis in infants: an underestimated disease. | the clinical diagnosis of pertussis is not easy in early infancy since clinical manifestations can overlap with several different diseases. many cases are often misclassified and underdiagnosed. we conducted a retrospective study on infants to assess how often physicians suspected pertussis and the actual frequency of bordetella pertussis infections. | 2016 | 27528377 |
| a diguanylate cyclase acts as a cell division inhibitor in a two-step response to reductive and envelope stresses. | cell division arrest is a universal checkpoint in response to environmental assaults that generate cellular stress. in bacteria, the cyclic di-gmp (c-di-gmp) signaling network is one of several signal transduction systems that regulate key processes in response to extra-/intracellular stimuli. here, we find that the diguanylate cyclase yfin acts as a bifunctional protein that produces c-di-gmp in response to reductive stress and then dynamically relocates to the division site to arrest cell divi ... | 2016 | 27507823 |
| enterovirus d68 infection in children with acute flaccid myelitis, colorado, usa, 2014. | during august 8, 2014-october 14, 2014, a total of 11 children with acute flaccid myelitis and distinctive neuroimaging changes were identified near denver, colorado, usa. a respiratory prodrome was experienced by 10, and nasopharyngeal specimens were positive for enterovirus d68 (ev-d68) for 4. to determine whether an association exists between ev-d68 infection and acute flaccid myelitis, we conducted a retrospective case-control study comparing these patients with 2 groups of outpatient contro ... | 2016 | 27434186 |
| a bordetella pertussis proteoliposome induces protection in mice without affecting the immunogenicity of diphtheria and tetanus toxoids in a trivalent formulation. | in this study, a formulation of bordetella pertussis proteoliposome (plbp), diphtheria, and tetanus toxoids and alum (dt-plbp) was evaluated as a trivalent vaccine candidate in balb/c mice. vaccine-induced protection was estimated using the intranasal challenge for pertussis and enzyme-linked immunosorbent assay fvto assess serological responses for diphtheria or tetanus. both, diphtheria-tetanus-whole cell pertussis (dtp) and diphtheria-tetanus vaccines (dt) were used as controls. animals immun ... | 2016 | 27489808 |
| vaccination strategies in patients with solid organ transplant: evidences and future perspectives. | solid organ transplant recipients need emphases on immunization that result in certainly decrease the risk of vaccine preventable diseases. organ transplant candidate should complete the recommended full vaccination schedule as early as possible during the courses of underlying disease because the patients with end stage liver or renal disease have reduced immune response to vaccine. furthermore, live attenuated vaccines are generally contraindicated after transplantation. this review summarizes ... | 2016 | 27489802 |
| influenza c infections in western australia and victoria from 2008 to 2014. | influenza c is usually considered a minor cause of respiratory illness in humans with many infections being asymptomatic or clinically mild. large outbreaks can occur periodically resulting in significant morbidity. | 2016 | 27373693 |
| control of clostridium difficile physiopathology in response to cysteine availability. | the pathogenicity of clostridium difficile is linked to its ability to produce two toxins: tcda and tcdb. the level of toxin synthesis is influenced by environmental signals, such as phosphotransferase system (pts) sugars, biotin, and amino acids, especially cysteine. to understand the molecular mechanisms of cysteine-dependent repression of toxin production, we reconstructed the sulfur metabolism pathways of c. difficile strain 630 in silico and validated some of them by testing c. difficile gr ... | 2016 | 27297391 |