Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| detection of impaired igg antibody formation facilitates the decision on early immunoglobulin replacement in hypogammaglobulinemic patients. | hypogammaglobulinemia (serum igg lower than 2 sd below the age-matched mean) and clinical symptoms such as increased susceptibility to infection, autoimmune manifestations, granulomatous disease, and unexplained polyclonal lymphoproliferation are considered to be diagnostic hallmarks in patients with common variable immunodeficiency (cvid), the most frequent clinically severe primary immunodeficiency syndrome. in the present study, we investigated patients with hypogammaglobulinemia and no clini ... | 2015 | 25699049 |
| preliminary study on the immunogenicity of a newly developed gcc tdap vaccine and its protection efficacy against bordetella pertussis in a murine intranasal challenge model. | active reduced dose tetanus-diphtheria-acellular pertussis (tdap) vaccination for adolescents and adults is necessary because waning immunity after primary diphtheria-tetanus-pertussis vaccination is related to the recent emergence of pertussis. this study was conducted to compare the immunogenicity and protection efficacy against bordetella pertussis between a new gcc tdap vaccine and a commercially available tdap vaccine in a murine model. | 2015 | 25649262 |
| association between sudden infant death syndrome and diphtheria-tetanus-pertussis immunisation: an ecological study. | sudden infant death syndrome (sids) continues to be one of the main causes of infant mortality in the united states. the objective of this study was to analyse the association between diphtheria-tetanus-pertussis (dtp) immunisation and sids over time. | 2015 | 25626628 |
| sustained macrophage infiltration upon multiple intra-articular injections: an improved rat model of rheumatoid arthritis for pet guided therapy evaluation. | to widen the therapeutic window for pet guided evaluation of novel anti-ra agents, modifications were made in a rat model of rheumatoid arthritis (ra). arthritis was induced in the right knee of wistar rats with repeated boosting to prolong articular inflammation. the contralateral knee served as control. after immunization with methylated bovine serum albumin (mbsa) in complete freund's adjuvant and custom bordetella pertussis antigen, one or more intra-articular (i.a.) mbsa injections were giv ... | 2015 | 25695087 |
| bordetella pertussis fim3 gene regulation by bvga: phosphorylation controls the formation of inactive vs. active transcription complexes. | two-component systems [sensor kinase/response regulator (rr)] are major tools used by microorganisms to adapt to environmental conditions. rr phosphorylation is typically required for gene activation, but few studies have addressed how and if phosphorylation affects specific steps during transcription initiation. we characterized transcription complexes made with rna polymerase and the bordetella pertussis rr, bvga, in its nonphosphorylated or phosphorylated (bvga∼p) state at p(fim3), the promot ... | 2015 | 25624471 |
| mycoplasma pneumoniae detection causes excess antibiotic use in norwegian general practice: a retrospective case-control study. | the 2011 mycoplasma pneumoniae epidemic in norway resulted in many gp consultations and significantly increased the prescription of macrolide antibiotics. | 2015 | 25624311 |
| clinical presentation and microbiological diagnosis in paediatric respiratory tract infection: a systematic review. | antibiotic prescribing decisions for respiratory tract infection (rti) in primary care could be improved if clinicians could target bacterial infections. however, there are currently no evidence-based diagnostic rules to identify microbial aetiology in children presenting with acute rtis. | 2015 | 25624310 |
| characterization of co-purified acellular pertussis vaccines. | whole-cell pertussis vaccines (wpvs) have been completely replaced by the co-purified acellular vaccines (apvs) in china. to date few laboratory studies were reported for co-purified apvs in terms of their antigenic composition and protective immune responses. to further understand the antigenic composition in co-purified apvs, in the present study 2-dimensional gel electrophoresis-based proteomic technology was used to analyze the composition of co-purified apvs. the results showed that besides ... | 2015 | 25610957 |
| combating pertussis resurgence: one booster vaccination schedule does not fit all. | pertussis has reemerged as a major public health concern in many countries where it was once considered well controlled. although the mechanisms responsible for continued pertussis circulation and resurgence remain elusive and contentious, many countries have nevertheless recommended booster vaccinations, the timing and number of which vary widely. here, using a stochastic, age-stratified transmission model, we searched for cost-effective booster vaccination strategies using a genetic algorithm. ... | 2015 | 25605878 |
| neutrophil-related factors as biomarkers in eae and ms. | a major function of t helper (th) 17 cells is to induce the production of factors that activate and mobilize neutrophils. although th17 cells have been implicated in the pathogenesis of multiple sclerosis (ms) and the animal model experimental autoimmune encephalomyelitis (eae), little attention has been focused on the role of granulocytes in those disorders. we show that neutrophils, as well as monocytes, expand in the bone marrow and accumulate in the circulation before the clinical onset of e ... | 2015 | 25559893 |
| fap2 of fusobacterium nucleatum is a galactose-inhibitable adhesin involved in coaggregation, cell adhesion, and preterm birth. | fusobacterium nucleatum is a common oral anaerobe involved in periodontitis that is known to translocate and cause intrauterine infections. in the oral environment, f. nucleatum adheres to a large diversity of species, facilitating their colonization and creating biological bridges that stabilize the multispecies dental biofilm. many of these interactions (called coadherences or coaggregations) are galactose sensitive. galactose-sensitive interactions are also involved in the binding of f. nucle ... | 2015 | 25561710 |
| approach to a child with recurrent pneumonia. | pneumonia, or inflammation of the lungs parenchyma associated with consolidation of alveolar spaces, is a substantial cause of morbidity and mortality in childhood particularly among children below 5 years of age. it is one of the common causes of admission to the paediatric ward. the aim of this article is to provide a guide to a systemic approach for diagnosis and treatment of children with recurrent pneumonia while not over investigating those with common but usually unrecognised conditions s ... | 2015 | 27493439 |
| high seroprevalence of bordetella pertussis in patients with chronic obstructive pulmonary disease: a case-control study. | bordetella pertussis has been suggested to take part in the acute exacerbation of chronic obstructive pulmonary disease (copd). the aim of this study was to investigate the association between b. pertussis and copd. | 2015 | 26858762 |
| infantile apparent life-threatening events, an educational review. | many physicians have received a frantic call from anxious parents stating that their child had stopped breathing, become limp, or turned blue but then had recovered quickly. an apparent life-threatening event (alte) is defined as "an episode that is frightening to the observer, and is characterized by some combination of apnea, color change, marked change in muscle tone, choking, gagging, or coughing". the incidence of alte is reported to be 0.05% to 6%. the knowledge about the most common cause ... | 2015 | 26512363 |
| whooping cough: identification, assessment and management. | although there is a high uptake of vaccinations providing protection against bordetella pertussis, the main cause of whooping cough, there has been an increase in the incidence of notifications of the disease in the uk and other developed countries in recent years. the increase in cases of whooping cough is mainly evident in older children and adults. while these individuals may experience persistent and unpleasant symptoms, most notably prolonged cough, symptoms may be mild, in part, because mo ... | 2016 | 24219484 |
| toll-like receptor 4 orchestrates neutrophil recruitment into airways during the first hours of bordetella pertussis infection. | most of the knowledge on the impact of bordetella pertussis lipo-oligosaccharide (los) on the infectious process was obtained when the bacteria was established within the host. the aim of the present work was to determine the role of tlr4 at a very early step of the infectious process. to this end we used a transcriptomic approach on b. pertussis intranasal infection model in c3h/hen, a tlr4-competent mouse strain, and c3h/hej, a tlr4-deficient mouse strain. the expression of approximately 140 g ... | 2016 | 23811096 |
| channel formation by rtx-toxins of pathogenic bacteria: basis of their biological activity. | the pore-forming cytolysins of the rtx-toxin (repeats in toxin) family are a relatively small fraction of a steadily increasing family of proteins that contain several functionally important glycine-rich and aspartate containing nonapeptide repeats. these cytolysins produced by a variety of gram-negative bacteria form ion-permeable channels in erythrocytes and other eukaryotic cells. hemolytic and cytolytic rtx-toxins represent pathogenicity factors of the toxin-producing bacteria and are very o ... | 2016 | 26523409 |
| inhibition of bacterial toxin activity by the nuclear stain, draq5™. | the repeats-in-toxin family of toxins includes proteins produced by gram negative bacteria such as escherichia coli (α-hemolysin), bordetella pertussis (adenylate cyclase toxin), and aggregatibacter actinomycetemcomitans (ltxa), which contribute to the pathogenesis of these organisms by killing host cells. in the case of ltxa produced by a. actinomycetemcomitans, white blood cells are targeted, allowing the bacteria to avoid clearance by the host immune system. in its association with target cel ... | 2016 | 27039399 |
| design and synthesis of fluorescent acyclic nucleoside phosphonates as potent inhibitors of bacterial adenylate cyclases. | bordetella pertussis adenylate cyclase toxin (act) and bacillus anthracis edema factor (ef) are key virulence factors with adenylate cyclase (ac) activity that substantially contribute to the pathogenesis of whooping cough and anthrax, respectively. there is an urgent need to develop potent and selective inhibitors of bacterial acs with prospects for the development of potential antibacterial therapeutics and to study their molecular interactions with the target enzymes. novel fluorescent 5-chlo ... | 2016 | 27775243 |
| comparison of three whole-cell pertussis vaccines in the baboon model of pertussis. | pertussis is a highly contagious respiratory illness caused by the bacterial pathogen bordetella pertussis. pertussis rates in the united states have escalated since the 1990s and reached a 50-year high of 48,000 cases in 2012. while this pertussis resurgence is not completely understood, we previously showed that the current acellular pertussis vaccines do not prevent colonization or transmission following challenge. in contrast, a whole-cell pertussis vaccine accelerated the rate of clearance ... | 2016 | 26561389 |
| cxcl14 as an emerging immune and inflammatory modulator. | cxcl14, a relatively novel chemokine, is a non-elr (glutamic acid-leucine-arginine) chemokine with a broad spectrum of biological activities. cxcl14 mainly contributes to the regulation of immune cell migration, also executes antimicrobial immunity. the identity of the receptor for cxcl14 still remains obscure and therefore the intracellular signaling pathway is not entirely delineated. the present review summarizes the contribution of cxcl14 in these two aspects and discusses the biological mec ... | 2016 | 26733763 |
| alternatives to hist for acellular pertussis vaccines: progress and challenges in replacement. | the 'international workshop on alternatives to the murine histamine sensitization test for acellular pertussis vaccines: progress and challenges in the replacement of hist' was held on 24 august 2014, in prague, czech republic, as a satellite meeting to the 9th world congress on alternatives and animal use in the life sciences. participants discussed the progress and challenges associated with the development, validation, and implementation of in vitro assays as replacements for the histamine se ... | 2016 | 27506225 |
| acute ischemic optic neuropathy with extended prone position ventilation in a lung transplant recipient. | prone position ventilation (ppv) improves mortality in severe acute respiratory distress syndrome (ards), but outcomes following its use in lung transplant recipients are not known. we report the case of a 42-year-old caucasian man who presented with severe ards from bordetella pertussis, 5 years after bilateral sequential lung transplant for cystic fibrosis. he was managed with ppv for 22 days and had a prolonged icu stay complicated by hypoxic ischemic optic neuropathy leading to blindness. si ... | 2016 | 27051622 |
| interdicting gq activation in airway disease by receptor-dependent and receptor-independent mechanisms. | gαqβγ heterotrimer (gq), an important mediator in the pathology of airway disease, plays a central role in bronchoconstriction and airway remodeling, including airway smooth muscle growth and inflammation. current therapeutic strategies to treat airway disease include the use of muscarinic and leukotriene receptor antagonists; however, these pharmaceuticals demonstrate a limited clinical efficacy as multiple gq-coupled receptor subtypes contribute to these pathologies. thus, broadly inhibiting t ... | 2016 | 26464325 |
| legionnaires' disease in south africa, 2012-2014. | during june 2012-september 2014, we tested patients with severe respiratory illness for legionella spp. infection and conducted a retrospective epidemiologic investigation. of 1,805 patients tested, legionella was detected in samples of 21 (1.2%); most were adults who had hiv or tuberculosis infections and were inappropriately treated for legionella. | 2016 | 26692504 |
| immunogenicity in dogs and protection against visceral leishmaniasis induced by a 14kda leishmania infantum recombinant polypeptide. | in areas were human visceral leishmaniasis (vl) is endemic, the domestic dog is the main parasite reservoir in the infectious cycle of leishmania infantum. development of prophylactic strategies to lower the parasite burden in dogs would reduce sand fly transmission thus lowering the incidence of zoonotic vl. here we demonstrate that vaccination of dogs with a recombinant 14kda polypeptide of l. infantum nuclear transport factor 2 (li-ntf2) mixed with adjuvant bpmpla-se resulted in the productio ... | 2016 | 26640609 |
| functional identification of putrescine c- and n-hydroxylases. | the small polyamine putrescine (1,4-diaminobutane) is ubiquitously and abundantly found in all three domains of life. it is a precursor, through n-aminopropylation or n-aminobutylation, for biosynthesis of the longer polyamines spermidine, sym-homospermidine, spermine, and thermospermine and longer and branched chain polyamines. putrescine is also biochemically modified for purposes of metabolic regulation and catabolism, e.g. n-acetylation and n-glutamylation, and for incorporation into special ... | 2016 | 27541336 |
| development of vaccines against pertussis caused by bordetella holmesii using a mouse intranasal challenge model. | bordetella holmesii is recognized as the third causative agent of pertussis (whooping cough) in addition to bordetella pertussis and bordetella parapertussis. pertussis caused by b. holmesii is not rare around the world. however, to date, there is no effective vaccine against b. holmesii. we examined the protective potency of pertussis vaccines available in japan and vaccines prepared from b. holmesii. a murine model of respiratory infection was exploited to evaluate protective potency. no japan ... | 2016 | 27515393 |
| bordetella holmesii: still emerging and elusive 20 years on. | since the first description of bordetella holmesii in 1995, almost 100 publications have contributed to the increasing knowledge of this emerging bacterium. although first reported to induce bacteremia mainly in immunocompromised patients, it has also been isolated in healthy persons and has shown the capacity to induce pertussis-like symptoms and other clinical entities, such as meningitis, arthritis, or endocarditis. respiratory diseases are generally less severe than those induced by bordetel ... | 2016 | 27227292 |
| periodic solutions in an sirws model with immune boosting and cross-immunity. | incidence of whooping cough, an infection caused by bordetella pertussis and bordetella parapertussis, has been on the rise since the 1980s in many countries. immunological interactions, such as immune boosting and cross-immunity between pathogens, have been hypothesised to be important drivers of epidemiological dynamics. we present a two-pathogen model of transmission which examines how immune boosting and cross-immunity can influence the timing and severity of epidemics. we use a combination ... | 2016 | 27575466 |
| two distinct episodes of whooping cough caused by consecutive bordetella pertussis and bordetella parapertussis infections in a fully immunized healthy boy. | we describe a 5-year-old, fully immunized boy with polymerase chain reaction-proven consecutive bordetella pertussis and bordetella parapertussis infections causing typical whooping cough at the age of 2 and 5 years, respectively. neither pertussis immunization nor disease provides reliable immunity against further episodes of whooping cough. | 2016 | 27391699 |
| bordetella pertussis. | pertussis is a highly infectious vaccine-preventable cough illness that continues to be a significant source of morbidity and mortality around the world. the majority of human illness is caused by bordetella pertussis, and some is caused by bordetella parapertussis. bordetella is a gram-negative, pleomorphic, aerobic coccobacillus. in the past several years, even countries with high immunization rates in early childhood have experienced rises in pertussis cases. reasons for the resurgence of rep ... | 2016 | 27337481 |
| incidence and diagnosis of pertussis in south african children hospitalized with lower respiratory tract infection. | the incidence of pertussis in children in low- and middle-income countries is poorly described. this study aimed to prospectively investigate the incidence of pertussis in south african children hospitalized with lower respiratory tract infection (lrti). | 2016 | 26967813 |
| structure activity characterization of bordetella petrii lipid a, from environment to human isolates. | bordetella petrii, a facultative anaerobic species, is the only known member of the bordetella genus with environmental origin. however it was also recently isolated from humans. the structures of the b. petrii lipid a moieties of the endotoxins were characterized here for the first time for an environmental strain and compared to that of human isolates. characterization was achieved using chemical analyses, gas chromatography-mass spectrometry, and matrix assisted laser desorption ionisation ma ... | 2016 | 26164553 |
| prevalence of bordetella holmesii and bordetella bronchiseptica in respiratory tract samples from belgian patients with pertussis-like symptoms by sensitive culture method and mass spectrometry. | insertion sequences is481 and is1001 are targets for molecular detection of respectively bordetella pertussis and bordetella parapertussis. there is a raising concern about specificity of these targets due to sequence similarity with bordetella holmesii and bordetella bronchiseptica. the likelihood of false (para)pertussis diagnoses should be correlated with the prevalence of these organisms in the respiratory tract (rt). from october 2010 until september 2011, 2,207 rt samples were submitted to ... | 2016 | 24579240 |
| prevalence of atypical pathogens in patients with cough and community-acquired pneumonia: a meta-analysis. | community-acquired pneumonia (cap), acute cough, bronchitis, and lower respiratory tract infections (lrti) are often caused by infections with viruses or streptococcus pneumoniae. the prevalence of atypical pathogens mycoplasma pneumoniae, chlamydophila pneumoniae, legionella pneumophila, and bordetella pertussis among patients with these illnesses in the ambulatory setting has not been previously summarized. we set out to derive prevalence information from the existing literature. | 2016 | 28376442 |
| live pertussis vaccines: will they protect against carriage and spread of pertussis? | pertussis is a severe respiratory disease that can be fatal in young infants. its main aetiological agent is the gram-negative micro-organism bordetella pertussis. vaccines against the disease have been in use since the 1950s, and global vaccination coverage has now reached more than 85%. nevertheless, the disease has not been controlled in any country, and has even made a spectacular come-back in the industrialized world, where the first-generation whole-cell vaccines have been replaced by the ... | 2016 | 28341014 |
| oxygen and bis(3',5')-cyclic dimeric guanosine monophosphate binding control oligomerization state equilibria of diguanylate cyclase-containing globin coupled sensors. | bacteria sense their environment to alter phenotypes, including biofilm formation, to survive changing conditions. heme proteins play important roles in sensing the bacterial gaseous environment and controlling the switch between motile and sessile (biofilm) states. globin coupled sensors (gcs), a family of heme proteins consisting of a globin domain linked by a central domain to an output domain, are often found with diguanylate cyclase output domains that synthesize c-di-gmp, a major regulator ... | 2016 | 27933792 |
| risk factors associated with bordetella pertussis among infants aged ≤4 months in the pre-tdap era - united states, 2002-2005. | in the united states, infants have the highest reported pertussis incidence and death rates. improved understanding of infant risk factors is needed to optimize prevention strategies. | 2016 | 28033240 |
| immunogenicity and safety of 10-valent pneumococcal non-typeable haemophilus influenzae protein d conjugate vaccine (phid-cv) administered to children with sickle cell disease between 8 weeks and 2 years of age: a phase iii, open, controlled study. | immunogenicity, safety and reactogenicity of the 10-valent pneumococcal non-typeable haemophilus influenzae protein d conjugate vaccine (phid-cv) were evaluated in children with sickle cell disease (scd), who are at increased risk for infections. | 2016 | 28030521 |
| [a case of bronchiolitis obliterans secondary to human metapneumovirus bronchiolitis]. | human metapneumovirus (hmpv), formerly classified in paramyxoviridae family is now moved into pneumoviridae, which was described as a novel family. it causes upper and lower respiratory tract infections (lrtis) usually in children younger than five years old. the recent epidemiological studies indicated that hmpv is the second most frequently detected virus in lrtis of young children, following the respiratory syncytial virus (rsv). bronchiolitis obliterans (bo) is a chronic obstructive lung dis ... | 2016 | 28124966 |
| pertussis in north-central and northwestern regions of algeria. | pertussis outbreaks continue to occur in many countries despite high vaccination coverage. under-diagnosed cases in adolescents and adults may result in increased transmission to infants, who are at risk of severe pertussis. additional measures to protect both groups should be considered. | 2016 | 27886031 |
| [trends in pertussis mortality and morbidity in colombia, 2002-2012]. | whooping cough is a public health problem that mainly affects children under one year of age with highly lethal outcomes. it is a re-emerging disease, which is preventable by immunization. objective: to analyze mortality and morbidity trends of whooping cough in colombia between 2002 and 2012. materials and methods: we conducted a quantitative descriptive study of deaths by pertussis between 2002 and 2012 using data from death certificates registered by the departamento administrativo nacional d ... | 2016 | 27869387 |
| hospital costs of bordetella pertussis in new zealand children. | to estimate hospitalisation costs for children with pertussis in new zealand. | 2016 | 27857241 |
| pneumomediastinum as a complication of critical pertussis. | pertussis is a common and potentially serious disease affecting mainly infants and young children. in its non-classic presentation, pertussis can be clinically indistinguishable from other respiratory illnesses. pertussis today often remains underdiagnosed in adults. our aims was to report a complicated cases of pertussis. | 2016 | 25764484 |
| aetiology and risk factors of community-acquired pneumonia in hospitalized patients in norway. | in norway, data on the aetiology of community-acquired pneumonia (cap) in hospitalized patients are limited. the aims of this study were to investigate the bacterial aetiology of cap in hospitalized patients in norway, risk factors for cap and possible differences in risk factors between patients with legionnaire's disease and pneumonia because of other causes. | 2016 | 25764275 |
| modeling the effects of priming with the whole-cell bordetella pertussis vaccine-reply. | 2016 | 27802485 | |
| modeling the effects of priming with the whole-cell bordetella pertussis vaccine. | 2016 | 27802457 | |
| immunogenicity and protective efficacy of recombinant iron superoxide dismutase protein from bordetella pertussis in mice models. | whooping cough (pertussis) is a highly contagious respiratory infection caused by bordetella pertussis. although availability of effective pertussis vaccines reportedly decreases the incidence of the disease, b. pertussis circulation in populations has not been eliminated. thus, it is necessary to find new protein candidates with greater immune protective capacities than the currently available acellular pertussis vaccines. in this study, iron superoxide dismutase (fesod) gene (sodb) was cloned, ... | 2016 | 27761933 |
| pertussis outbreak among patients and healthcare workers in a provincial dialysis facility in japan. | sixteen pertussis cases in haemodialysis patients and healthcare workers were detected in a 25-bed outpatient haemodialysis facility in japan between october 2013 and april 2014. | 2016 | 27760708 |
| bordetella pertussis modulates human macrophage defense gene expression. | bordetella pertussis, the etiological agent of whooping cough, still causes outbreaks. we recently found evidence that b. pertussis can survive and even replicate inside human macrophages, indicating that this host cell might serve as a niche for persistence. in this work, we examined the interaction of b. pertussis with a human monocyte cell line (thp-1) that differentiates into macrophages in culture in order to investigate the host cell response to the infection and the mechanisms that promot ... | 2016 | 27465637 |
| adaptive immune response to whole cell pertussis vaccine reflects vaccine quality: a possible complementation to the pertussis serological potency test. | whole cell bordetella pertussis (wp) vaccines are still used in many countries to protect against the respiratory disease pertussis. the potency of whole-cell pertussis vaccine lots is determined by an intracerebral challenge test (the kendrick test). this test is criticized due to lack of immunological relevance of the read-out after an intracerebral challenge with b. pertussis. the alternative in vivo test, which assesses specific antibody levels in serum after wp vaccination, is the pertussis ... | 2016 | 27452867 |
| evaluation of commercial assays for single-point diagnosis of pertussis in the us. | pertussis serodiagnosis is increasingly being used in the united states despite the lack of a us food and drug administration-approved, commercially available assay. to better understand the utility of these assays in diagnosing pertussis, serology assays were evaluated for analytical parameters and clinical accuracy. | 2016 | 27451419 |
| vitamins as influenza vaccine adjuvant components. | a number of adjuvant formulations were assayed in mice immunized with 3.75 µg of a/california/7/2009 (h1n1) pdm09 influenza vaccine with vitamins a, d and/or e in emulsions or b2 and/or b9 combined with bordetella pertussis mpla and/or alum as adjuvants. squalene was used as positive control, as well as mpla with alum. the immune response was evaluated by a panel of tests, including a hemagglutination inhibition (hai) test, elisa for igg, igg1, and igg2a and ifn-γ, il-2, il-6 and il-10 quantific ... | 2016 | 27449155 |
| comparison of point-of-care-compatible lysis methods for bacteria and viruses. | nucleic acid sample preparation has been an especially challenging barrier to point-of-care nucleic acid amplification tests in low-resource settings. here we provide a head-to-head comparison of methods for lysis of, and nucleic acid release from, several pathogenic bacteria and viruses-methods that are adaptable to point-of-care usage in low-resource settings. digestion with achromopeptidase, a mixture of proteases and peptidoglycan-specific hydrolases, followed by thermal deactivation in a bo ... | 2016 | 27424294 |
| new horizons in the development of novel needle-free immunization strategies to increase vaccination efficacy. | the young twenty-first century has already brought several medical advances, such as a functional artificial human liver created from stem cells, improved antiviral (e.g., against hiv) and cancer (e.g., against breast cancer) therapies, interventions controlling cardiovascular diseases, and development of new and optimized vaccines (e.g., hpv vaccine). however, despite this substantial progress and the achievements of the last century, humans still suffer considerably from diseases, especially f ... | 2016 | 27370343 |
| intracellular disassembly and activity of pertussis toxin require interaction with atp. | the active subunit (s1) of pertussis toxin (pt), a major virulence factor of bordetella pertussis, adp-ribosylates gi proteins in the mammalian cell cytosol to inhibit gpcr signaling. the intracellular pathway of pt includes endocytosis and retrograde transport to the trans-golgi network (tgn) and endoplasmic reticulum (er). subsequent translocation of s1 to the cytosol is presumably preceded by dissociation from the holotoxin. in vitro, such dissociation is stimulated by interaction of pt with ... | 2016 | 27369899 |
| burden and outcomes of severe pertussis infection in critically ill infants. | despite world health organization endorsed immunization schedules, bordetella pertussis continues to cause severe infections, predominantly in infants. there is a lack of data on the frequency and outcome of severe pertussis infections in infants requiring icu admission. we aimed to describe admission rates, severity, mortality, and costs of pertussis infections in critically ill infants. | 2016 | 27362854 |
| better colonisation of newly emerged bordetella pertussis in the co-infection mouse model study. | molecular epidemiological data indicates that the resurgence of pertussis (whooping cough) in populations with high vaccine coverage is associated with genomic adaptation of bordetella pertussis, the causative agent of the disease, to vaccine selection pressure. we have previously shown that in the period after the introduction of acellular pertussis vaccine (acv), the majority of circulating strains in australia switched to single nucleotide polymorphism (snp) cluster i (carrying ptxp3/prn2), r ... | 2016 | 27346304 |
| purification design and practice for pertactin, the third component of acellular pertussis vaccine, from bordetella pertussis. | development of acellular pertussis vaccine (apv) requires purification of several components from bordetella pertussis. while the components pertussis toxin (pt) and filamentous hemagglutinin (fha) have been successfully purified, the third component, pertactin, proves to be a difficult target due to its very low concentration. in order to solve its purification problem, we performed the surface potential analysis with grasp2 program. the results demonstrated that there are two major charge patc ... | 2016 | 27302339 |
| pertussis: history of the disease and current prevention failure. | pertussis or whooping cough has been given many names over the centuries. it was first recognized in the middle ages and since then various epidemics have been described. jules bordet and octave gengou isolated bordetella pertussis, a causative agent for whooping cough, in paris more than 100 years ago, which created an excellent opportunity to invent a vaccine. in 1914 the whole-cell pertussis vaccine was invented, then in the 1940s it was combined with tetanus and diphtheria toxoids to become ... | 2016 | 27256351 |
| gtl001, a therapeutic vaccine for women infected with human papillomavirus 16 or 18 and normal cervical cytology: results of a phase i clinical trial. | women infected with human papillomavirus (hpv) with normal cytology to mild abnormalities currently have no treatment options other than watchful waiting or surgery if high-grade cervical lesions or cancer develop. a therapeutic vaccine would offer the possibility of preventing high-grade lesions in hpv-infected women. gtl001 is a therapeutic vaccine composed of recombinant hpv16 and hpv18 e7 proteins fused to catalytically inactive bordetella pertussis cyaa. this study examined the tolerability ... | 2016 | 27252412 |
| pertussis vaccine effectiveness in the setting of pertactin-deficient pertussis. | in the united states, the proportion of bordetella pertussis isolates lacking pertactin, a component of acellular pertussis vaccines, increased from 14% in 2010 to 85% in 2012. the impact on vaccine effectiveness (ve) is unknown. | 2016 | 27244813 |
| surveillance of pertussis: methods and implementation. | pertussis or whooping cough is a respiratory disease caused by bordetella pertussis or, to a lesser extent, by b. parapertussis. vaccines against pertussis have been widely used for more than 50 years and have led to a significant reduction of morbidity and mortality. however, even in countries with a high vaccine coverage, the disease is still not well controlled. surveillance is urgently needed. | 2016 | 27224518 |
| infectious disease report: bordetella pertussis infection in patients with cancer. | we illustrate 2 cases of pneumonia associated with bordetella pertussis infection in 72-year-old and 61-year-old patients with cancer receiving myelosuppressive therapy after hematopoietic stem cell transplantation. bacterial infections are a significant cause of morbidity and mortality in patients with cancer, and those receiving hematopoietic stem cell transplant, solid organ transplant, or myelosuppressive therapy are at increased risk. the infection was detected and the 2 patients had good o ... | 2016 | 27218794 |
| evolution of bordetella pertussis. | 2016 | 27213264 | |
| pertussis: where did we go wrong and what can we do about it? | pertussis or whooping cough, mainly caused by the gram-negative coccobacillus bordetella pertussis, is a severe respiratory disease that can by life-threatening especially in young infants. it has recently made a spectacular come-back in high vaccination-coverage countries, such as the us, australia and many european countries. although a trend towards increased pertussis incidence was already visible before the switch from whole-cell to acellular vaccines, it was really since the introduction o ... | 2016 | 27161992 |
| characterization of the immune response induced by pertussis omvs-based vaccine. | for the development of a third generation of pertussis vaccine that could improve the control of the disease, it was proposed that the immune responses induced by the classic whole cell vaccine (wp) or after infection should be used as a reference point. we have recently identified a vaccine candidate based on outer membrane vesicles (omvs) derived from the disease etiologic agent that have been shown to be safe and protective in mice model of infection. here we characterized omvs-mediated immun ... | 2016 | 27151884 |
| direct molecular typing of bordetella pertussis from nasopharyngeal specimens in china in 2012-2013. | data on the molecular epidemiology of bordetella pertussis are limited in developing countries where whole-cell pertussis vaccines (wcvs) have been used. the aim of this study was to determine the genotypes of circulating b. pertussis in china by direct molecular typing of clinical specimens. dna extracts of 122 nasopharyngeal swabs (nps) positive for b. pertussis by polymerase chain reaction (pcr) (targeting is481 and ptx-pr) from 2012 to 2013 were used for typing using the multiple-locus varia ... | 2016 | 27146879 |
| immunization against pertussis in adolescents and adults. | pertussis is a highly contagious infectious disease caused by bordetella pertussis that can be extremely serious, particularly in young infants. for many years the efforts of health authorities throughout the world to prevent pertussis had the main goals of reducing the morbidity of infants and children under 5 years of age, maintaining protection for several years during the school-age period and developing a significant herd immunity to directly and indirectly reduce the risk of the spread of ... | 2016 | 27130670 |
| aetiology of childhood pneumonia in a well vaccinated south african birth cohort: a nested case-control study of the drakenstein child health study. | pneumonia is a leading cause of mortality and morbidity in children globally. the cause of pneumonia after introduction of the 13-valent pneumococcal conjugate vaccine (pcv13) has not been well studied in low-income and middle-income countries, and most data are from cross-sectional studies of children admitted to hospital. we aimed to longitudinally investigate the incidence and causes of childhood pneumonia in a south african birth cohort. | 2016 | 27117547 |
| serodiagnosis of whooping cough in belgium: results of the national reference centre for bordetella pertussis anno 2013. | report on the pitfalls of serodiagnosis of pertussis in belgium for 2013 by the nrc bordetella. | 2016 | 27075799 |
| evidence of increased circulation of bordetella pertussis in the italian adult population from seroprevalence data (2012-2013). | incidence data on pertussis cases in italy do not show pertussis resurgence, as recently described in other european countries. the aim of this study was to determine the seroprevalence of igg antibodies to pertussis toxin (pt-igg) in selected adult age groups, which can serve as a reservoir of b. pertussis and be responsible for onward transmission to vulnerable infants. the seroprevalence of pt-igg was studied in sera collected in 2012-13 in age groups 20-29, 30-39 (reproductive age), and ≥ 60 ... | 2016 | 27075481 |
| development of a gene delivery system in streptococcus gordonii using thymidylate synthase as a selection marker. | streptococcus gordonii, a commensal bacterium of the human oral cavity, is a potential live vaccine vector. in this study, we have developed a system that delivers a vaccine antigen gene onto the chromosome of s. gordonii. the system consisted of a recipient strain, that is a thymidine auxotroph constructed by deletion of a portion of thya gene, and a linear gene delivery construct, composed of the functional thya gene, the vaccine antigen gene, and a dna fragment immediately downstream of thya. ... | 2016 | 27062990 |
| calcium-driven folding of rtx domain β-rolls ratchets translocation of rtx proteins through type i secretion ducts. | calcium-binding rtx proteins are equipped with c-terminal secretion signals and translocate from the ca(2+)-depleted cytosol of gram-negative bacteria directly into the ca(2+)-rich external milieu, passing through the "channel-tunnel" ducts of type i secretion systems (t1sss). using bordetella pertussis adenylate cyclase toxin, we solved the structure of an essential c-terminal assembly that caps the rtx domains of rtx family leukotoxins. this is shown to scaffold directional ca(2+)-dependent fo ... | 2016 | 27058787 |
| pertussis: microbiology, disease, treatment, and prevention. | pertussis is a severe respiratory infection caused by bordetella pertussis, and in 2008, pertussis was associated with an estimated 16 million cases and 195,000 deaths globally. sizeable outbreaks of pertussis have been reported over the past 5 years, and disease reemergence has been the focus of international attention to develop a deeper understanding of pathogen virulence and genetic evolution of b. pertussis strains. during the past 20 years, the scientific community has recognized pertussis ... | 2016 | 27029594 |
| epidemiological and economic effects of priming with the whole-cell bordetella pertussis vaccine. | current acellular pertussis vaccines may not protect against transmission of bordetella pertussis. | 2016 | 27018830 |
| a novel igm-capture enzyme-linked immunosorbent assay using recombinant vag8 fusion protein for the accurate and early diagnosis of bordetella pertussis infection. | an elisa that measures anti-pt igg antibody has been used widely for the serodiagnosis of pertussis; however, the igg-based elisa is inadequate for patients during the acute phase of the disease because of the slow response of anti-pt igg antibodies. to solve this problem, we developed a novel igm-capture elisa that measures serum anti-bordetella pertussis vag8 igm levels for the accurate and early diagnosis of pertussis. first, we confirmed that vag8 was highly expressed in all b. pertussis iso ... | 2016 | 26996337 |
| effect of different detoxification procedures on the residual pertussis toxin activities in vaccines. | pertussis toxin (ptx) is a major virulence factor produced by bordetella pertussis and its detoxified form is one of the major protective antigens in vaccines against whooping cough. ideally, ptx in the vaccine should be completely detoxified while still preserving immunogenicity. however, this may not always be the case. due to multilevel reaction mechanisms of chemical detoxification that act on different molecular sites and with different production processes, it is difficult to define a mole ... | 2016 | 26973066 |
| the optimal gestation for pertussis vaccination during pregnancy: a prospective cohort study. | there is an increasing incidence of pertussis infection in infants too young to be adequately protected via vaccination. maternal pertussis vaccination during the third trimester of pregnancy is a new strategy to provide protection to newborn infants. | 2016 | 26968625 |
| [toward a new immunization schedule in spain, 2016 (part 2)]. | immunization schedules are intrinsically dynamic in order to embed the immunologic and epidemiologic changes in any specific geographic region. according to this, the current study addresses a proposal to modify the childhood immunization schedule in spain. in order to move from a three plus one schema to a two plus one, we undertake a review of the available literature to explore the immunological and clinical rationale behind this change, including an overview of the potential impact on this s ... | 2016 | 26960345 |
| [toward a new immunization schedule in spain, 2016 (part 1)]. | the immunization schedule is a dynamic public health tool that has incorporated different changes over the years influenced by the epidemiologic situation and the scientific evidence. the immunization advisory committee [ponencia de programa y registro de vacunaciones], as the interterritorial council scientific and technical advisory body, carries out assessments of different programmes and vaccines and proposes changes that after approval will be introduced in the regions schedule. this articl ... | 2016 | 26960253 |
| [persistence of bordetella pertussis bacteria and a possible mechanism of its formation]. | a growth of pertussis morbidity is observed in many countries of the world against the background of mass vaccindtion. forms of the disease course have changed. atypical forms of pertussis occur predominately in adolescents and adults. asymptomatic carriage of the causative agent has been established. infection of infants with. bordetelia pertussis bacteria in more than 90% of cases occurs from parents and relatives. a prolonged persistence of the causative agent has been identified. morbidity i ... | 2016 | 26951000 |
| bordetella pertussis epidemiology and evolution in the light of pertussis resurgence. | whooping cough, or pertussis, is resurgent in many countries world-wide. this is linked to switching from the use of whole cell vaccines to acellular vaccines in developed countries. current evidence suggests that this has resulted in the earlier waning of vaccine-induced immunity, an increase in asymptomatic infection with concomitant increases in transmission and increased selection pressure for bordetellapertussis variants that are better able to evade vaccine-mediated immunity than older iso ... | 2016 | 26932577 |
| comparison of the immunogenicity and safety of pentavalent vaccine quinvaxem in a compact prefilled auto-disabled (cpad) injection system versus single-dose vials in healthy infants: a phase 3, open-label, randomized, parallel-group, non-inferiority study. | to evaluate non-inferiority of three doses of quinvaxem in a compact prefilled auto-disabled (cpad) injection system versus quinvaxem in a single-dose vial administered with conventional syringe in terms of seroconversion/seroprotection rates for all antibodies (anti-hepatitis b (hb), anti-haemophilus influenzae type b polyribosylribitol phosphate (hib prp), anti-diphtheria, anti-tetanus, anti-bordetella pertussis) at 1 month after primary vaccination. | 2016 | 26923083 |
| antigenic and genetic characterization of bordetella pertussis recovered from quebec, canada, 2002-2014: detection of a genetic shift. | despite vaccination, cyclical peaks of bordetella pertussis incidence rates are still observed in canada and other developed countries, making pertussis one of the most prevalent vaccine preventable bacterial diseases. in the postacellular vaccine era, evolution of bacterial strains has resulted in strains with altered vaccine antigens. previous canadian studies have focused on isolates mainly from the provinces of ontario and alberta, with only small numbers of isolates from other provinces. th ... | 2016 | 26910633 |
| the role of b. pertussis vaccine antigen gene variants in pertussis resurgence and possible consequences for vaccine development. | whooping cough, or pertussis, caused by bordetella pertussis is considered resurgent in a number of countries world-wide, despite continued high level vaccine coverage. among a number of causes for this that have been proposed, is the emergence of b. pertussis strains expressing variants of the antigens contained in acellular pertussis vaccines; i.e. the evolution of b. pertussis toward vaccine escape. this commentary highlights the contradictory nature of evidence for this but also discusses th ... | 2016 | 26889694 |
| monitoring of an antigen manufacturing process. | fluorescence spectroscopy in combination with multivariate statistical methods was employed as a tool for monitoring the manufacturing process of pertactin (prn), one of the virulence factors of bordetella pertussis utilized in whopping cough vaccines. fluorophores such as amino acids and co-enzymes were detected throughout the process. the fluorescence data collected at different stages of the fermentation and purification process were treated employing principal component analysis (pca). throu ... | 2016 | 26879644 |
| proteome analysis of bordetella pertussis isolated from human macrophages. | previous studies have shown that b. pertussis survives inside human macrophages in non-acidic compartments with characteristics of early endosomes. in order to gain new insight into the biology of b. pertussis survival in host cells, we have analyzed the adaptation of the bacterial proteome during intracellular infection. the proteome of b. pertussis 3 h and 48 h after infection of human macrophage-like thp-1 cells was examined by nano-liquid chromatography combined with tandem ms and compared t ... | 2016 | 26873878 |
| porcelain lung: calcification in severe bordetella pertussis infection. | 2016 | 26868038 | |
| differences in bordetella pertussis dna load according to clinical and epidemiological characteristics of patients with whooping cough. | to identify associations between nasopharyngeal bordetella pertussis dna load and clinical and epidemiological characteristics and evaluate dna load prognostic value in pertussis severity. | 2016 | 26850358 |
| camp signalling of bordetella adenylate cyclase toxin through the shp-1 phosphatase activates the bimel-bax pro-apoptotic cascade in phagocytes. | the adenylate cyclase toxin-hemolysin (cyaa, act or ac-hly) plays a key role in virulence of bordetella pertussis. cyaa penetrates myeloid cells expressing the complement receptor 3 (αm β2 integrin cd11b/cd18) and subverts bactericidal capacities of neutrophils and macrophages by catalysing unregulated conversion of cytosolic atp to the key signalling molecule adenosine 3',5'-cyclic monophosphate (camp). we show that the signalling of cyaa-produced camp hijacks, by an as yet unknown mechanism, t ... | 2016 | 26334669 |
| an ecological analysis of pertussis disease in minnesota, 2009-2013. | the increase in pertussis cases in minnesota in the last decade has been mainly attributed to the switch from whole cell to acellular pertussis [as part of the diphtheria, tetanus and acellular pertussis vaccine (dtap)]. it is unclear, however, to what degree community-level risk factors also contribute. understanding these factors can help inform public health policy-makers about where else to target resources. we performed an ecological analysis within minnesota to identify risk factors at the ... | 2016 | 26330135 |
| latent class analysis of diagnostic tests for adenovirus, bordetella pertussis and influenza virus infections in german adults with longer lasting coughs. | laboratory tests in adult outpatients with longer lasting coughs to identify a potential causal pathogen are rarely performed, and there is no gold standard for these diagnostic tests. while the diagnostic validity of serological tests for pertussis is well established their potential contribution for diagnosing adenovirus and influenza virus a and b infections is unclear. a sentinel study into the population-based incidence of longer lasting coughs in adults was done in rostock (former east ger ... | 2016 | 26380914 |
| restricted antibody response to bordetella pertussis filamentous hemagglutinin induced by whole-cell and acellular pertussis vaccines. | filamentous hemagglutinin (fha) is a principal virulence factor, an important immunogenic antigen of bordetella pertussis, and a major component of many acellular pertussis vaccines. in the present study, the human antibody response to different regions of fha was determined in healthy children and adults vaccinated with either whole-cell or acellular pertussis vaccines. | 2016 | 26439274 |
| pore-formation by adenylate cyclase toxoid activates dendritic cells to prime cd8+ and cd4+ t cells. | the adenylate cyclase toxin-hemolysin (cyaa) of bordetella pertussis is a bi-functional leukotoxin. it penetrates myeloid phagocytes expressing the complement receptor 3 and delivers into their cytosol its n-terminal adenylate cyclase enzyme domain (~400 residues). in parallel, ~1300 residue-long rtx hemolysin moiety of cyaa forms cation-selective pores and permeabilizes target cell membrane for efflux of cytosolic potassium ions. the non-enzymatic cyaa-ac(-) toxoid, has repeatedly been successf ... | 2016 | 26437769 |
| [pertussis in mexico, an epidemiological overview. a study of 19 years at the instituto mexicano del seguro social]. | bordetella pertussis infection remains a public health problem in several developed and developing we describe the epidemiological syndrome cases subsystem special surveillance of whooping cough from 1992 to 2011 at a population with social security. | 2016 | 25760745 |
| understanding the true burden and infection dynamics of bordetella pertussis using molecular diagnostics. | 2016 | 26826519 | |
| genomic dissection of australian bordetella pertussis isolates from the 2008-2012 epidemic. | despite high pertussis vaccination coverage, australia experienced a prolonged epidemic in 2008-2012. the predominant bordetella pertussis genotype harboured pertussis toxin promoter allele, ptxp3, and pertactin gene allele, prn2. the emergence and expansion of prn non-expressing isolates (prn negative), were also observed. we aimed to investigate the microevolution and genomic diversity of epidemic b. pertussis isolates. | 2016 | 26826518 |
| whooping cough in a renal transplant recipient. | whooping cough is a respiratory infection with a severity that varies with age, immune status, and probably with other factors such as the degree of exposure and the virulence of the organism. the most frequent microorganism responsible for whooping cough is bordetella pertussis. we present the case of a 62-year-old renal transplant recipient presenting with typical and severe manifestations of whooping cough caused by b. pertussis. | 2016 | 26808962 |