Publications

nuclear export inhibitors avert progression in preclinical models of inflammatory demyelination.	axonal damage has been associated with aberrant protein trafficking. we examined a newly characterized class of compounds that target nucleo-cytoplasmic shuttling by binding to the catalytic groove of the nuclear export protein xpo1 (also known as crm1, chromosome region maintenance protein 1). oral administration of reversible crm1 inhibitors in preclinical murine models of demyelination significantly attenuated disease progression, even when started after the onset of paralysis. clinical effic ...	2015	25706475
role of exopolysaccharide in aggregatibacter actinomycetemcomitans-induced bone resorption in a rat model for periodontal disease.	aggregatibacter actinomycetemcomitans a causative agent of periodontal disease in humans, forms biofilm on biotic and abiotic surfaces. a. actinomycetemcomitans biofilm is heterogeneous in nature and is composed of proteins, extracellular dna and exopolysaccharide. to explore the role played by the exopolysaccharide in the colonization and disease progression, we employed genetic reduction approach using our rat model of a. actinomycetemcomitans-induced periodontitis. to this end, a genetically ...	2015	25706999
sudden infant death syndrome and the genetics of inflammation.	several studies report signs of slight infection prior to death in cases of sudden infant death syndrome (sids). based on this, a hypothesis of an altered immunological homeostasis has been postulated. the cytokines are important cellular mediators that are crucial for infant health by regulating cell activity during the inflammatory process. the pro-inflammatory cytokines favor inflammation; the most important of these are il-1α, il-1β, il-6, il-8, il-12, il-18, tnf-α, and ifn-γ. these cytokine ...	2015	25750641
whole-genome sequence of a bordetella pertussis brazilian vaccine strain.	despite the reduction in incidence after vaccination, pertussis disease is still considered a public health problem worldwide, mainly due to recent and potential new outbreaks. we report here the complete genome of the bordetella pertussis butantan strain used in the brazilian national immunization program as a whole-cell pertussis antigen to compose vaccines such as dtwp (diphtheria, tetanus, and whole-cell pertussis).	2015	25700409
β-hydroxybutyric sodium salt inhibition of growth hormone and prolactin secretion via the camp/pka/creb and ampk signaling pathways in dairy cow anterior pituitary cells.	β-hydroxybutyric acid (bhba) regulates the synthesis and secretion of growth hormone (gh) and prolactin (prl), but its mechanism is unknown. in this study, we detected the effects of bhba on the activities of g protein signaling pathways, ampk-α activity, gh, and prl gene transcription, and gh and prl secretion in dairy cow anterior pituitary cells (dcapcs). the results showed that bhba decreased intracellular camp levels and a subsequent reduction in protein kinase a (pka) activity. inhibition ...	2015	25690038
vaccination in children with allergy to non active vaccine components.	childhood immunisation is one of the greatest public health successes of the last century. vaccines contain an active component (the antigen) which induces the immune response. they may also contain additional components such as preservatives, additives, adjuvants and traces of other substances. this review provides information about risks of hypersensitivity reactions to components of vaccines. furthermore, recommendations to avoid or reduce reactions to vaccine components have been detailed.	2015	25852819
transcriptional profiling of bordetella pertussis reveals requirement of rna chaperone hfq for type iii secretion system functionality.	bordetella pertussis, the causative agent of human whooping cough (pertussis) produces a complex array of virulence factors in order to establish efficient infection in the host. the rna chaperone hfq and small regulatory rnas are key players in posttranscriptional regulation in bacteria and have been shown to play an essential role in virulence of a broad spectrum of bacterial pathogens. this study represents the first attempt to characterize the hfq regulon of the human pathogen b. pertussis u ...	2015	25674816
biographical feature: raymond c. bartlett, m.d.		2015	25673787
characterization of interactions between inclusion membrane proteins from chlamydia trachomatis.	chlamydiae are obligate intracellular pathogens of eukaryotes. the bacteria grow in an intracellular vesicle called an inclusion, the membrane of which is heavily modified by chlamydial proteins called incs (inclusion membrane proteins). incs represent 7-10% of the genomes of chlamydia and, given their localization at the interface between the host and the pathogen, likely play a key role in the development and pathogenesis of the bacterium. however, their functions remain largely unknown. here, ...	2015	25717440
multiple driving forces required for efficient secretion of autotransporter virulence proteins.	autotransporter (at) proteins are a broad class of virulence proteins from gram-negative bacterial pathogens that require their own c-terminal transmembrane domain to translocate their n-terminal passenger across the bacterial outer membrane (om). but given the unavailability of atp or a proton gradient across the om, it is unknown what energy source(s) drives this process. here we used a combination of computational and experimental approaches to quantitatively compare proposed at om translocat ...	2015	25670852
high-affinity σ1 protein agonist reduces clinical and pathological signs of experimental autoimmune encephalomyelitis.	selective agonists of the sigma-1 receptor (σ1 protein) are generally reported to protect against neuronal damage and modulate oligodendrocyte differentiation. human and rodent lymphocytes possess saturable, high-affinity binding sites for compounds binding to the σ1 protein and potential immunomodulatory properties have been described for σ1 protein ligands. experimental autoimmune encephalomyelitis (eae) is recognized as a valuable model of the inflammatory aspects of multiple sclerosis (ms). ...	2015	25521311
dna recognition by escherichia coli cbpa protein requires a conserved arginine-minor-groove interaction.	curved dna binding protein a (cbpa) is a co-chaperone and nucleoid associated dna binding protein conserved in most γ-proteobacteria. best studied in escherichia coli, cbpa accumulates to >2500 copies per cell during periods of starvation and forms aggregates with dna. however, the molecular basis for dna binding is unknown; cbpa lacks motifs found in other bacterial dna binding proteins. here, we have used a combination of genetics and biochemistry to elucidate the mechanism of dna recognition ...	2015	25670677
identification and functional characterization of the novel edwardsiella tarda effector esej.	edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and gastro- and extraintestinal infections in humans. the type iii secretion system (t3ss) of e. tarda has been identified as a key virulence factor that contributes to pathogenesis in fish. however, little is known about the associated effectors translocated by this t3ss. in this study, by comparing the profile of secreted proteins of the wild-type ppd130/91 and its t3ss atpase δesan mutant, we ide ...	2015	25667268
retrospective study of the clinical epidemiological characteristics of pertussis in infants prior to their first vaccination in the russian federation.	the coverage of pediatric pertussis vaccination in the russian federation is high, generally using a diphtheria, tetanus, and whole-cell pertussis vaccine in a 3-, 4.5-, and 6-month primary series and with a booster at 18 months of age. however, with no registered pertussis vaccines for adults, unvaccinated adolescents and adults can be a major source of infection of infants under 3 months of age.	2015	25663041
pertussis outbreak in university students and evaluation of acellular pertussis vaccine effectiveness in japan.	recent studies worldwide have reported increasing numbers of adults diagnosed with bordetella pertussis despite receiving childhood vaccinations. this study describes a pertussis outbreak at a university medical faculty campus and examines the effectiveness of diphtheria, tetanus, and pertussis (dtap) vaccination completed during infancy in japan.	2015	25656486
ifn-β treatment requires b cells for efficacy in neuroautoimmunity.	ifn-β remains the most widely prescribed treatment for relapsing remitting multiple sclerosis. despite widespread use of ifn-β, the therapeutic mechanism is still partially understood. particularly, the clinical relevance of increased b cell activity during ifn-β treatment is unclear. in this article, we show that ifn-β pushes some b cells into a transitional, regulatory population that is a critical mechanism for therapy. ifn-β treatment increases the absolute number of regulatory cd19(+)cd24(+ ...	2015	25646307
research on bacterial virulence in the developing countries.		2015	25713813
n-terminal and c-terminal domains of calmodulin mediate fadd and tradd interaction.	fadd (fas-associated death domain) and tradd (tumor necrosis factor receptor 1-associated death domain) proteins are important regulators of cell fate in mammalian cells. they are both involved in death receptors mediated signaling pathways and have been linked to the toll-like receptor family and innate immunity. here we identify and characterize by database search analysis, mutagenesis and calmodulin (cam) pull-down assays a calcium-dependent cam binding site in the α-helices 1-2 of tradd deat ...	2015	25643035
detection of impaired igg antibody formation facilitates the decision on early immunoglobulin replacement in hypogammaglobulinemic patients.	hypogammaglobulinemia (serum igg lower than 2 sd below the age-matched mean) and clinical symptoms such as increased susceptibility to infection, autoimmune manifestations, granulomatous disease, and unexplained polyclonal lymphoproliferation are considered to be diagnostic hallmarks in patients with common variable immunodeficiency (cvid), the most frequent clinically severe primary immunodeficiency syndrome. in the present study, we investigated patients with hypogammaglobulinemia and no clini ...	2015	25699049
preliminary study on the immunogenicity of a newly developed gcc tdap vaccine and its protection efficacy against bordetella pertussis in a murine intranasal challenge model.	active reduced dose tetanus-diphtheria-acellular pertussis (tdap) vaccination for adolescents and adults is necessary because waning immunity after primary diphtheria-tetanus-pertussis vaccination is related to the recent emergence of pertussis. this study was conducted to compare the immunogenicity and protection efficacy against bordetella pertussis between a new gcc tdap vaccine and a commercially available tdap vaccine in a murine model.	2015	25649262
association between sudden infant death syndrome and diphtheria-tetanus-pertussis immunisation: an ecological study.	sudden infant death syndrome (sids) continues to be one of the main causes of infant mortality in the united states. the objective of this study was to analyse the association between diphtheria-tetanus-pertussis (dtp) immunisation and sids over time.	2015	25626628
sustained macrophage infiltration upon multiple intra-articular injections: an improved rat model of rheumatoid arthritis for pet guided therapy evaluation.	to widen the therapeutic window for pet guided evaluation of novel anti-ra agents, modifications were made in a rat model of rheumatoid arthritis (ra). arthritis was induced in the right knee of wistar rats with repeated boosting to prolong articular inflammation. the contralateral knee served as control. after immunization with methylated bovine serum albumin (mbsa) in complete freund's adjuvant and custom bordetella pertussis antigen, one or more intra-articular (i.a.) mbsa injections were giv ...	2015	25695087
bordetella pertussis fim3 gene regulation by bvga: phosphorylation controls the formation of inactive vs. active transcription complexes.	two-component systems [sensor kinase/response regulator (rr)] are major tools used by microorganisms to adapt to environmental conditions. rr phosphorylation is typically required for gene activation, but few studies have addressed how and if phosphorylation affects specific steps during transcription initiation. we characterized transcription complexes made with rna polymerase and the bordetella pertussis rr, bvga, in its nonphosphorylated or phosphorylated (bvga∼p) state at p(fim3), the promot ...	2015	25624471
mycoplasma pneumoniae detection causes excess antibiotic use in norwegian general practice: a retrospective case-control study.	the 2011 mycoplasma pneumoniae epidemic in norway resulted in many gp consultations and significantly increased the prescription of macrolide antibiotics.	2015	25624311
clinical presentation and microbiological diagnosis in paediatric respiratory tract infection: a systematic review.	antibiotic prescribing decisions for respiratory tract infection (rti) in primary care could be improved if clinicians could target bacterial infections. however, there are currently no evidence-based diagnostic rules to identify microbial aetiology in children presenting with acute rtis.	2015	25624310
characterization of co-purified acellular pertussis vaccines.	whole-cell pertussis vaccines (wpvs) have been completely replaced by the co-purified acellular vaccines (apvs) in china. to date few laboratory studies were reported for co-purified apvs in terms of their antigenic composition and protective immune responses. to further understand the antigenic composition in co-purified apvs, in the present study 2-dimensional gel electrophoresis-based proteomic technology was used to analyze the composition of co-purified apvs. the results showed that besides ...	2015	25610957
combating pertussis resurgence: one booster vaccination schedule does not fit all.	pertussis has reemerged as a major public health concern in many countries where it was once considered well controlled. although the mechanisms responsible for continued pertussis circulation and resurgence remain elusive and contentious, many countries have nevertheless recommended booster vaccinations, the timing and number of which vary widely. here, using a stochastic, age-stratified transmission model, we searched for cost-effective booster vaccination strategies using a genetic algorithm. ...	2015	25605878
neutrophil-related factors as biomarkers in eae and ms.	a major function of t helper (th) 17 cells is to induce the production of factors that activate and mobilize neutrophils. although th17 cells have been implicated in the pathogenesis of multiple sclerosis (ms) and the animal model experimental autoimmune encephalomyelitis (eae), little attention has been focused on the role of granulocytes in those disorders. we show that neutrophils, as well as monocytes, expand in the bone marrow and accumulate in the circulation before the clinical onset of e ...	2015	25559893
fap2 of fusobacterium nucleatum is a galactose-inhibitable adhesin involved in coaggregation, cell adhesion, and preterm birth.	fusobacterium nucleatum is a common oral anaerobe involved in periodontitis that is known to translocate and cause intrauterine infections. in the oral environment, f. nucleatum adheres to a large diversity of species, facilitating their colonization and creating biological bridges that stabilize the multispecies dental biofilm. many of these interactions (called coadherences or coaggregations) are galactose sensitive. galactose-sensitive interactions are also involved in the binding of f. nucle ...	2015	25561710
approach to a child with recurrent pneumonia.	pneumonia, or inflammation of the lungs parenchyma associated with consolidation of alveolar spaces, is a substantial cause of morbidity and mortality in childhood particularly among children below 5 years of age. it is one of the common causes of admission to the paediatric ward. the aim of this article is to provide a guide to a systemic approach for diagnosis and treatment of children with recurrent pneumonia while not over investigating those with common but usually unrecognised conditions s ...	2015	27493439
high seroprevalence of bordetella pertussis in patients with chronic obstructive pulmonary disease: a case-control study.	bordetella pertussis has been suggested to take part in the acute exacerbation of chronic obstructive pulmonary disease (copd). the aim of this study was to investigate the association between b. pertussis and copd.	2015	26858762
infantile apparent life-threatening events, an educational review.	many physicians have received a frantic call from anxious parents stating that their child had stopped breathing, become limp, or turned blue but then had recovered quickly. an apparent life-threatening event (alte) is defined as "an episode that is frightening to the observer, and is characterized by some combination of apnea, color change, marked change in muscle tone, choking, gagging, or coughing". the incidence of alte is reported to be 0.05% to 6%. the knowledge about the most common cause ...	2015	26512363
sequence variation in virulence-related genes of bordetella pertussis isolates from poland in the period 1959-2013.	this study aimed to characterise bordetella pertussis isolates circulating in poland since 1959. sequence analysis of ptxa, ptxc, prn, tcfa, fim2, fim3 and ptxp for 175 clinical isolates and currently and previously used vaccine strains was performed. clinical isolates from the period 1995-2013 were found to be different to three currently used vaccine strains harbouring the allelic combination ptxa2-ptxc1-ptxp1-prn1-tcfa2-fim2-1-fim3-1, seen frequently in poland in the early pertussis vaccinati ...	2015	25090968
pertactin-negative bordetella pertussis strains: evidence for a possible selective advantage.	a recent increase in bordetella pertussis without the pertactin protein, an acellular vaccine immunogen, has been reported in the united states. determining whether pertactin-deficient (prn(-)) b. pertussis is evading vaccine-induced immunity or altering the severity of illness is needed.	2015	25301209
colonization of bordetella pertussis clinical isolates that differ by pulsed field gel electrophoresis types in the lungs of naïve mice or mice immunized with the whole-cell pertussis vaccine used in poland.	the goal of our study was to compare the elimination of bordetella pertussis clinical isolates that differ according to pulsed field gel electrophoresis (pfge), serotypes and genes encoding virulence factors from the lungs of naïve mice or mice immunized with commercial diphtheria-tetanus-whole-cell pertussis vaccine used in poland. when a mixture of four isolates, given in equal proportions and harboring different pfge profiles, serotypes, and alleles encoding virulence factors, was used to inf ...	2015	25297678
a novel tlr2 agonist from bordetella pertussis is a potent adjuvant that promotes protective immunity with an acellular pertussis vaccine.	bordetella pertussis causes whooping cough, a severe and often lethal respiratory infection in infants. a recent resurgence of pertussis has been linked with waning or suboptimal immunity induced with acellular pertussis vaccines (pa) that were introduced to most developed countries in the 1990s because of safety concerns around the use of whole-cell pertussis vaccines (pw). pa are composed of individual b. pertussis antigens absorbed to alum and promote strong antibody, t helper type 2 (th2) an ...	2015	25315966
pertussis may be the cause of prolonged cough in adolescents and adults in the interepidemic period.	this study was aimed to evaluate the prevalence of pertussis in adolescents and adults with cough lasting more than 14 days and less than 30 days.	2015	25452019
pertussis serosurveillance study in izmir, turkey.	pertussis is a life-threatening, vaccine-preventable infection. adults who can be asymptomatic may infect infants. the aim of this study is to determine the igg antibody levels against pertussis toxin (pt) and filamentous hemagglutinin from 6 months to ≥60 years in izmir, turkey. a cluster sample design developed by expanded programme on immunization of the world health organization was carried out for the selection of the study population, which consisted of 399 healthy subjects. in-house elisa ...	2015	25424662
a critical role for the tlr signaling adapter mal in alveolar macrophage-mediated protection against bordetella pertussis.	bordetella pertussis causes whooping cough, an infectious disease of the respiratory tract that is re-emerging despite high vaccine coverage. here we examined the role of toll-like receptor (tlr) adapter protein mal in the control of b. pertussis infection in the lungs. we found that b. pertussis bacterial load in the lungs of mal-defective (mal(-/-)) mice exceeded that of wild-type (wt) mice by up to 100-fold and bacteria disseminated to the liver in mal(-/-) mice and 50% of these mice died fro ...	2015	25515629
genomic analysis of isolates from the united kingdom 2012 pertussis outbreak reveals that vaccine antigen genes are unusually fast evolving.	a major outbreak of whooping cough, or pertussis, occurred in 2012 in the united kingdom (uk), with nearly 10 000 laboratory-confirmed cases and 14 infant deaths attributed to pertussis. a worldwide resurgence of pertussis has been linked to switch to the use of acellular pertussis vaccines and the evolution of bordetella pertussis away from vaccine-mediated immunity. we have conducted genomic analyses of multiple strains from the uk outbreak. we show that the uk outbreak was polyclonal in natur ...	2015	25489002
modulation of the cd4(+) t cell response after acellular pertussis vaccination in the presence of tlr4 ligation.	whole cell pertussis (wp) vaccines are gradually being replaced by aluminum salt-adjuvanted acellular pertussis (ap) vaccines. these promote cd4(+) t cell responses with a non-protective th2 component, while protective immune mechanisms to b. pertussis may rather involve long-lived th1/th17 type cd4(+) t cells. here we asked whether addition of a non-toxic meningococcal lps derivative, lpxl1, as adjuvant can favorably modulate the ap-induced pertussis-specific cd4(+) t cell response in mice. to ...	2015	25659267
in vitro innate immune cell based models to assess whole cell bordetella pertussis vaccine quality: a proof of principle.	lot release testing of vaccines is primarily based on animal models that are costly, time-consuming and sometimes of questionable relevance. in order to reduce animal use, functional in vitro assays are being explored as an alternative approach for the current lot release testing paradigm. in this study, we present an evaluation of apc platforms assessing innate immune activation by whole cell bordetella pertussis (wp) vaccines. primary monocytes, monocyte-derived dc (modc) and human monocyte/dc ...	2015	25633359
antibody levels to bordetella pertussis and neisseria meningitidis in immunodeficient patients receiving immunoglobulin replacement therapy.	patients with antibody deficiency rely on immunoglobulin products for protection against many vaccine-preventable diseases. we measured antibody titers against bordetella pertussis and neisseria meningitidis in patients receiving immunoglobulin (ig) therapy to determine if they have any protection against infections from these organisms.	2015	25631528
resurgence of pertussis at the age of vaccination: clinical, epidemiological, and molecular aspects.	report the incidence, epidemiology, clinical features, death, and vaccination status of patients with whooping cough and perform genotypic characterization of isolates of b. pertussis identified in the state of paraná, during january 2007 to december 2013.	2015	25623040
pertussis in the newborn: certainties and uncertainties in 2014.	bordetella pertussis infection remains a serious potential health risk to infants, specially in those too young to be vaccinated. over the recent years, numerous sources highlighted a widespread resurgence, making it, again, a challenging disease. globally, pertussis is ranked among the 10 leading causes of childhood mortality. this review summarizes the most recent literature and will address the most important aspects that pediatricians and neonatologists must be familiar with, when treating a ...	2015	25613084
notes from the field: investigation of contacts of a health care worker who worked while ill with pertussis--maryland, august-september 2014.	on september 5, 2014, the public health department of a maryland hospital was notified of a case of bordetella pertussis infection confirmed by polymerase chain reaction (pcr) in a staff health care worker (hcw). the hcw experienced onset of diarrhea and malaise (nonrespiratory symptoms atypical of the catarrhal phase of pertussis) on august 26. by september 2, paroxysms of coughing led the hcw to consult a colleague, who ordered the pcr test, prescribed a 5-day course of azithromycin, and advis ...	2015	25611170
comparison of loop-mediated isothermal amplification and real-time pcr for detecting bordetella pertussis.		2015	25596118
carrot cells: a pioneering platform for biopharmaceuticals production.	carrot (daucus carota l.) is of importance in the molecular farming field as it constitutes the first plant species approved to produce biopharmaceuticals for human use. in this review, features that make carrot an advantageous species in the molecular farming field are analyzed and a description of the developments achieved with this crop thus far is presented. a guide for genetic transformation procedures is also included. the state of the art comprises ten vaccine prototypes against measles v ...	2015	25572939
interaction of bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding.	the interaction of bordetella pertussis adenylate cyclase toxin (cyaa) with complement receptor 3 (cr3, cd11b/cd18) involves n-linked oligosaccharide chains. to investigate the relative importance of the individual n-glycans of cr3 for toxin activity, the asparagine residues of the consensus n-glycosylation sites of cr3 were substituted with glutamine residues that cannot be glycosylated. examination of cr3 mutant variants and mass spectrometry analysis of the n-glycosylation pattern of cr3 reve ...	2015	25554420
epidemiology of pertussis in adolescents and adults in turkey.	two hundred and fourteen patients who had a cough illness lasting at least 2 weeks were studied to investigate bordetella pertussis as a cause of prolonged cough in adolescents and adults. medical history and nasopharyngeal swab specimens for culture and polymerase chain reaction (pcr) were obtained at presentation. three (1·4%) patients were b. pertussis culture-positive; 15 (7%) were b. pertussis pcr-positive (including the culture-positive patients) and 11 (5·1%) were bordetella spp. pcr-posi ...	2015	25524454
bordetella pertussis diagnosis in children under five years of age in the regional hospital of cajamarca, northern peru.	bordetella pertussis is an important human pathogen that causes whooping cough (pertussis), an endemic illness responsible of significant morbidity and mortality, especially in infants and children. worldwide, there are an estimated of 16 million cases of pertussis, resulting in about 195,000 child deaths per year. in peru, pertussis is a major health problem that has been on the increase despite immunization efforts. the objective of this study was to determine the prevalence of b. pertussis am ...	2015	26623626
eight-year review of bordetella pertussis testing reveals seasonal pattern in the united states.	review of bordetella pertussis polymerase chain reaction testing from 2007 through 2014 revealed a yearly spike in positivity rates during the summer throughout the united states. paradoxically, the highest test volumes occurred outside of this time frame, which provides an opportunity for improved test utilization.	2015	26621328
[clinical analysis of 247 children with whooping cough and the risk factors of severe cases].	to summarize the clinical characteristics of whooping cough in children and analyze the risk factors for severe whooping cough.	2015	26757969
temperature accelerated molecular dynamics with soft-ratcheting criterion orients enhanced sampling by low-resolution information.	many proteins exhibit an equilibrium between multiple conformations, some of them being characterized only by low-resolution information. visiting all conformations is a demanding task for computational techniques performing enhanced but unfocused exploration of collective variable (cv) space. otherwise, pulling a structure toward a target condition biases the exploration in a way difficult to assess. to address this problem, we introduce here the soft-ratcheting temperature-accelerated molecula ...	2015	26575778
protecting newborns against pertussis: treatment and prevention strategies.	pertussis is a potentially severe respiratory disease, which affects all age groups from young infants to older adults and is responsible for an estimated 195,000 deaths occurred globally in 2008. active research is ongoing to better understand the pathogenesis, immunology, and diagnosis of pertussis. for diagnosis, molecular assays (e.g., polymerase chain reaction) for detection of bordetella pertussis have become more widely available and support improved outbreak detection. in children, pertu ...	2015	26542059
detection of bordetella pertussis using a pcr test in infants younger than one year old hospitalized with whooping cough in five peruvian hospitals.	to report the incidence, epidemiology, and clinical features of bordetella pertussis in peruvian infants under 1 year old.	2015	26523641
community awareness and predictors of uptake of pertussis booster vaccine in south australian adults.	pertussis is a highly virulent vaccine preventable disease that remains a global challenge. this study aimed to assess community knowledge of pertussis infection as well as awareness and uptake of adult pertussis booster vaccine.	2015	26514422
[pertussis (whooping cough)--an update].	whooping cough is a highly contagious respiratory disease which is caused predominantly by the gram-negative bacterium bordetella pertussis. further bordetella species such as b. parapertussis and the recently discovered species b. holmesii are also involved in whooping cough-like diseases. depending on age, vaccination status and distance to pre-infection with b. pertussis, whooping cough shows a wide range of symptoms. the disease occurs at any age, leaving only short time immunity. during the ...	2015	26837155
isolated cyaa-rtx subdomain from bordetella pertussis: structural and functional implications for its interaction with target erythrocyte membranes.	the 126-kda bordetella pertussis cyaa-hemolysin (cyaa-hly) was previously expressed in escherichia coli as a soluble precursor that can be acylated to retain hemolytic activity. here, we investigated structural and functional characteristics of a ∼100-kda isolated rtx (repeat-in-toxin) subdomain (cyaa-rtx) of cyaa-hly. initially, we succeeded in producing a large amount with high purity of the his-tagged cyaa-rtx fragment and in establishing the interaction of acylated cyaa-hly with sheep red bl ...	2015	26325465
the impact of parental postpartum pertussis vaccination on infection in infants: a population-based study of cocooning in western australia.	during a pertussis epidemic in 2011-2012 the western australian (wa) department of health implemented a 'cocooning' programme, offering free pertussis-containing vaccine (dtpa) to new parents. we assessed the impact of vaccinating parents with dtpa on the incidence of pertussis infection in newborns. births in wa during 2011-2012 were linked to a register of parental pertussis vaccinations and to notified reports of laboratory-proven pertussis in children <6 months of age. parents who received d ...	2015	26320420
estimated and reported incidence of pertussis in estonian adults: a seroepidemiological study.	rates of pertussis immunisation among children in estonia are high (∼95%), but pertussis is still the most common vaccine preventable childhood disease. adults are suspected to be sources of pertussis in children. we aimed to measure pertussis toxin (pt) igg in adults to estimate pertussis infection activity and compare estimated and reported pertussis incidences.	2015	26277068
frequency and characteristics of hospital-sponsored pertussis revaccination programs in the southern united states.	the centers for disease control and prevention (cdc) released guidelines in december, 2006, recommending revaccination against bordetella pertussis with tdap for all post-partum women and healthcare workers. the cdc recommendations specifically state "the postpartum tdap should be administered before discharge from the hospital or birthing center" and "hospitals and ambulatory-care facilities should provide tdap for healthcare personnel." the purpose of this survey was to determine the frequency ...	2015	26272891
t-cell immune responses to bordetella pertussis infection and vaccination.	the recent immunological investigations, stemming from the studies performed in the nineties within the clinical trials of the acellular pertussis vaccines, have highlighted the important role played by t-cell immunity to pertussis in humans. these studies largely confirmed earlier investigations in the murine respiratory infection models that humoral immunity alone is not sufficient to confer protection against bordetella pertussis infection and that t-cell immunity is required. over the last y ...	2015	26242279
seroprevalence studies of pertussis: what have we learned from different immunized populations.	bordetella pertussis is a pathogen-causing whooping cough (pertussis) in humans. although vaccination against the disease is effective, the bacterium is still circulating among population and can even cause death. especially young infants, who lack protection, are vulnerable. the laboratory diagnostic methods to detect b. pertussis are culture, pcr and elisa serology. reported cases of the disease vary among countries but usually the incidence rates are low, <1 to 10/100 000. however, pertussis ...	2015	26208655
the potential of adjuvants to improve immune responses against tdap vaccines: a preclinical evaluation of mf59 and monophosphoryl lipid a.	the successful approach of combining diphtheria, tetanus and pertussis antigens into a single vaccine has become a cornerstone of immunization programs. yet, even if vaccination coverage is high, a resurgence of pertussis has been reported in many countries suggesting current vaccines may not provide adequate protection. to induce better tailored and more durable immune responses against pertussis vaccines different approaches have been proposed, including the use of novel adjuvants. licensed ap ...	2015	26149936
bisamidate prodrugs of 2-substituted 9-[2-(phosphonomethoxy)ethyl]adenine (pmea, adefovir) as selective inhibitors of adenylate cyclase toxin from bordetella pertussis.	novel small-molecule agents to treat bordetella pertussis infections are highly desirable, as pertussis (whooping cough) remains a serious health threat worldwide. in this study, a series of 2-substituted derivatives of 9-[2-(phosphonomethoxy)ethyl]adenine (pmea, adefovir), in their isopropyl ester bis(l-phenylalanine) prodrug form, were designed and synthesized as potent inhibitors of adenylate cyclase toxin (act) isolated from b. pertussis. the series consists of pmea analogues bearing either ...	2015	26136378
detection of antibodies against fimbria type 3 (fim3) is useful diagnostic assay for pertussis.	isolation of bordetella pertussis and detection of the pertussis genome are not always successful because of low bacterial loads in adult patients with pertussis. antibodies against pertussis toxin (pt) are measured but have low sensitivity in vaccinated subjects. there is no reliable diagnostic method at present. in this study, a fluorescent-eia against several pertussis antigens and genome detection were investigated to establish clinical laboratory diagnostic methods for pertussis. the study ...	2015	26134278
respiratory infection with a bacterial pathogen attenuates cns autoimmunity through il-10 induction.	infection with viral or bacterial pathogens has been linked with the development of multiple sclerosis (ms), while infection with helminth parasites has been associated protection against ms and other autoimmune diseases. here we have used a murine model of ms, experimental autoimmune encephalomyelitis (eae), to examine the effect of infection with the respiratory pathogen bordetella pertussis infection on development of cns inflammation. the data demonstrate that infection of mice with b. pertu ...	2015	26100487
needle-free and adjuvant-free epicutaneous boosting of pertussis immunity: preclinical proof of concept.	the limited durability of pertussis vaccine-induced protection requires novel approaches to reactivate immunity and limit pertussis resurgence in older children and adults. we propose that periodic boosters could be delivered using a novel epicutaneous delivery system (viaskin) to deliver optimized pertussis antigens such as genetically-detoxified pertussis toxin (rpt). to best mimic the human situation in which vaccine-induced memory cells persist, whereas antibodies wane, we developed a novel ...	2015	26067183
a field study of household attack rates and the effectiveness of macrolide antibiotics in reducing household transmission of pertussis.	bordetella pertussis (whooping cough) is an endemic, highly contagious bacterial respiratory infection, which is notifiable to australian state and territory health departments. between 2008 and 2011 there was a substantial outbreak in new south wales with an initial increase in cases occurring in north coast new south wales from late 2007. during september and october 2011 the north coast public health unit conducted a household study of secondary attack rates to assess the effectiveness of per ...	2015	26063095
bordetella pertussis isolates circulating in china where whole cell vaccines have been used for 50 years.		2015	26060284
[vaccinations from the pulmonologist's point of view].	the best strategy for prevention of acute respiratory tract infections is primary prophylaxis against diseases preventable by vaccination. from the pulmonologist's point of view, vaccinations against pneumococci, influenza a and b viruses and bordetella pertussis are of particular clinical relevance.	2015	26059891
whooping cough, twenty years from acellular vaccines introduction.	clinical pertussis resulting from infection with b. pertussis is a significant medical and public health problem, despite the huge success of vaccination that has greatly reduced its incidence. the whole cell vaccine had an undeniable success over the last 50 years, but its acceptance was strongly inhibited by fear, only partially justified, of severe side effects, but also, in the western world, by the difficulty to enter in combination with other vaccines: today multi-vaccine formulations are ...	2015	26051141
competition, coinfection and strain replacement in models of bordetella pertussis.	pertussis, or whooping cough, is an important respiratory infection causing considerable infant mortality worldwide. recently, incidence has risen in countries with strong vaccine programmes and there are concerns about antigenic shift resulting in vaccine evasion. interactions between pertussis and non-vaccine-preventable strains will play an important role in the evolution and population dynamics of pertussis. in particular, if we are to understand the role strain replacement plays in vaccinat ...	2015	26032910
a versatile, non genetically modified organism (gmo)-based strategy for controlling low-producer mutants in bordetella pertussis cultures using antigenic modulation.	the uncontrolled presence of non-producer mutants negatively affects bioprocesses. in bordetella pertussis cultures, avirulent mutants emerge spontaneously and accumulate. we characterized the dynamics of accumulation using high-throughput growth assays and competition experiments between virulent and avirulent (bvg(-) ) isolates. a fitness advantage of bvg(-) cells was identified as the main driver for bvg(-) accumulation under conditions of high virulence factor production. conversely, under c ...	2015	26014907
immunoproteomic profiling of bordetella pertussis outer membrane vesicle vaccine reveals broad and balanced humoral immunogenicity.	the current resurgence of whooping cough is alarming, and improved pertussis vaccines are thought to offer a solution. outer membrane vesicle vaccines (omvpv) are potential vaccine candidates, but omvpv-induced humoral responses have not yet been characterized in detail. the purpose of this study was to determine the antigen composition of omvpv and to elucidate the immunogenicity of the individual antigens. quantitative proteome analysis revealed the complex composition of omvpv. the omvpv immu ...	2015	25988566
pertussis resurgence associated with pertactin-deficient and genetically divergent bordetella pertussis isolates in israel.	the bordetella pertussis polymerase chain reaction positivity rate changed after additional diphtheria-tetanus-acellular pertussis boosters in 2005 and 2008, 9.8%, 13.4%, 22% and 15.2% in 2010, 2011, 2012 and 2013, p < 0.001, respectively. new pulsed-field gel electrophoresis profiles were detected between 2009 and 2012. the proportion of pertactin-deficient isolates increased over time, 6.6% versus 7.1% versus 33.3% during 2005-2006, 2011-2012 and 2013-2014, p < 0.03, respectively.	2015	25966915
host-pathogen interaction during bacterial vaccination.	vaccines have been developed and deployed against several important bacterial pathogens of humans, including neisseria meningitidis, bordetella pertussis, streptococcus pneumoniae and mycobacterium tuberculosis. these vaccines are generally considered a successful public health measure and are effective at controlling disease symptoms and/or burden. however, a troubling consequence of recent vaccination programs has been the selection of vaccine escape mutants, whereby the pathogen displays a di ...	2015	25966310
structural and functional studies of bapc protein of bordetella pertussis.	bordetella pertussis, the causative agent of whooping cough, attaches to mucosal surface in upper respiratory tract, where it produces a variety of surface associated and secreted autotransporter molecules among others. in this study we have cloned newly identified member of autotransporter family bapc (b. pertussis autotransporter protein c); expressed it in escherichia coli and characterized it for its different properties. we have also raised antisera to bapc protein; the antisera were used i ...	2015	25946329
bordetella pertussis infection in paediatric healthcare workers.	an increased incidence of pertussis has been observed recently in adults, and healthcare workers (hcws) are considered a risk group for transmission to infants. prevalence of recent pertussis infection was assessed in hcws from a paediatric department of a tertiary care hospital in brazil. serum pertussis toxin igg antibodies were measured by enzyme-linked immunosorbent assay. of 388 hcws included in the analysis, 6.4% had serology suggestive of recent infection. medical residents [odds ratio (o ...	2015	25913647
bordetella pertussis adenylate cyclase toxin blocks induction of bactericidal nitric oxide in macrophages through camp-dependent activation of the shp-1 phosphatase.	the adenylate cyclase toxin-hemolysin (cyaa) plays a key role in the virulence of bordetella pertussis. cyaa penetrates complement receptor 3-expressing phagocytes and catalyzes uncontrolled conversion of cytosolic atp to the key second messenger molecule camp. this paralyzes the capacity of neutrophils and macrophages to kill bacteria by complement-dependent oxidative burst and opsonophagocytic mechanisms. we show that camp signaling through the protein kinase a (pka) pathway activates src homo ...	2015	25876760
minimum inhibitory concentrations of erythromycin and other antibiotics for czech strains of bordetella pertussis.	to test the susceptibility to first-line and alternative antibiotics of 70 bordetella pertussis (b. pertussis) strains recovered from patients with whooping cough through national pertussis surveillance in the czech republic (cr) in 1967-2010.	2015	25872990
bordetella pertussis infection among international travellers: the need for a rigorous implementation of vaccine policies.		2015	25864792
molecular epidemiology of bordetella pertussis in the philippines in 2012-2014.	the present study was designed to determine the genotypes of circulating bordetella pertussis in the philippines by direct molecular typing of clinical specimens.	2015	25861926
importance of enhanced surveillance for prevention of pertussis in children.	bordetella pertussis infection causes considerable morbidity, even in countries with high vaccination coverage. surveillance of pertussis is usually passive and based on mandatory reporting. we assessed the epidemiologic and clinical characteristics of pertussis cases detected by passive or enhanced surveillance.	2015	25860533
biochemical and functional characterization of a periplasmic disulfide oxidoreductase from neisseria meningitidis essential for meningococcal viability.	tlpas (thioredoxin-like proteins) are bacterial thioredoxin-like periplasmic disulfide oxidoreductases generally involved in cytochrome c maturation (ccm) process. they contain a characteristic cxxc active site motif involved in disulfide exchange reaction. in the human pathogenic neisseria meningitidis species, no tlpa has been characterized so far. in the present study, using an in silico analysis, we identified a putative periplasmic tlpa, called tlpa2. biochemical and kinetic characterizatio ...	2015	25826614
implementation of pertussis immunization in health-care personnel.	infection with bordetella pertussis is most severe in young infants who frequently acquire it from adults. pertussis immunization in adults 25-29 years of age and all adults in close contact with infants <6 months was introduced in switzerland in 2012. we immediately implemented this new recommendation in our hospital with a vaccination campaign.	2015	25776922
[pertussis vaccines: acellular versus whole cell. perhaps a return to the past?].	the resurgence of pertussis in the world and in our country has questioned the effectiveness of cellular and acellular vaccines. the reason why pertussis has not been controlled or eliminated after 70 years of implementation of the vaccination is probably multifactorial. this article, on the basis of questions and answers, describes the benefits and limitations of both cellular and acellular vaccines and suggests new strategies of vaccination in childhood. it is a fact that the currently applied ...	2015	26633113
a cocktail of humanized anti-pertussis toxin antibodies limits disease in murine and baboon models of whooping cough.	despite widespread vaccination, pertussis rates are rising in industrialized countries and remain high worldwide. with no specific therapeutics to treat disease, pertussis continues to cause considerable infant morbidity and mortality. the pertussis toxin is a major contributor to disease, responsible for local and systemic effects including leukocytosis and immunosuppression. we humanized two murine monoclonal antibodies that neutralize pertussis toxin and expressed them as human immunoglobulin ...	2015	26631634
[antigenic variability of bordetella pertussis strains isolated in 1967-2010 in the czech republic--possible explanation for the rise in cases of pertussis?].	comparison of antigenic structures of bordetella pertussis (b. pertussis) strains isolated from 1967 to 2010 in the czech republic.	2015	26448300
pertactin negative bordetella pertussis demonstrates higher fitness under vaccine selection pressure in a mixed infection model.	whooping cough or pertussis is a highly infectious respiratory disease in humans caused by bordetella pertussis. the use of acellular vaccines (acv) has been associated with the recent resurgence of pertussis in developed countries including australia despite high vaccination coverage where b. pertussis strains that do not express pertactin (prn), a key antigenic component of the acv, have emerged and become prevalent. in this study, we used an in vivo competition assay in mice immunised with ac ...	2015	26432908
an in vitro assay for substrate translocation by fhac in liposomes.	the two-partner secretion (tps) pathway is used by gram-negative bacteria to secrete a large family of virulence exoproteins. its name is derived from the fact that it involves two proteins, a secreted tpsa protein and a cognate tpsb transporter in the outer membrane. a typical tps system is represented by the filamentous hemagglutinin fhab (tpsa protein) and its transporter fhac (tpsb protein) of bordetella pertussis. results from mutational analysis and heterologous expression experiments sugg ...	2015	26427679
genetic diversity and population dynamics of bordetella pertussis in china between 1950-2007.	pertussis is an acute respiratory infectious disease caused by the bacterium bordetella pertussis. although pertussis vaccination was introduced in the 1960s, pertussis is still an endemic disease in china. to better understand the genetic diversity of the chinese b. pertussis population, we characterized 115 clinical isolates obtained in china during 1950-2007 using multilocus variable-number tandem repeat analysis (mlva). forty-six different b. pertussis mlva profiles (mts) were identified, of ...	2015	26409140
[bordetella pertussis lipooligosaccharide-derived neoglycoconjugates - new components of pertussis vaccine].	pertussis is a contagious respiratory tract disease caused by the gram-negative bacterium bordetella pertussis. despite widespread vaccination, in recent years the pertussis incidence has increased. the whole-cell pertussis vaccine has been very effective but reactogenic. therefore the improved vaccines contain only a few isolated and inactivated antigens of b. pertussis. however, a waning of the acellular vaccine-induced immunity indicates that these vaccines lack some important protective b. p ...	2015	26400888
a newborn with pertussis accompanying nephrotic syndrome.	pertussis or whooping cough is a vaccine-preventable disease that still remains a serious infection in neonates and young infants. the disease is particularly severe in infants less than three months old, who are often infected by their parents. congenital nephrotic syndrome is a rare entity presenting within the first three months. it encompasses a heterogeneous group of entities with genetic, infectious and idiopathic etiologies. in this report we describe a newborn infant who presented with c ...	2015	26388601
pertussis in children in the istanbul faculty of medicine: results for four years.	we investigated the frequency of pertussis among children in the istanbul faculty of medicine hospital during a period of four years. clinical specimens were obtained from children who exhibited symptoms of whooping cough; a portion of the cases were confirmed microbiologically by pcr as pertussis. a total of 410 nasopharyngeal aspirates were taken for detection of bordetella pertussis/parapertussis. the age groups of the patients were 0-4 months (n=201), >4 -12 months (n=49), 1-4 years (n=79), ...	2015	26388594
functional importance of the gly cluster in transmembrane helix 2 of the bordetella pertussis cyaa-hemolysin: implications for toxin oligomerization and pore formation.	adenylate cyclase-hemolysin (cyaa) is a major virulence factor of bordetella pertussis causing whooping cough in humans. we previously showed that two transmembrane helices (α2 and α3) in the hemolysin domain (cyaa-hly) are crucially involved in hemolytic activity. here, pcr-based substitutions were employed to investigate a potential involvement in hemolysis of a series of four gly residues (gly(530), gly(533), gly(537) and gly(544)) which map onto one face of a helical wheel plot of pore-linin ...	2015	26363293
pertussis in the era of new strains of bordetella pertussis.	despite implementation of a successful vaccination program, pertussis remains a significant health problem. although the incidence of pertussis in the united states is reduced by approximately 80% compared with incidence before the introduction of vaccination in the 1940s, deaths still occur and the unrecognized disease burden remains high, with 1 million bordetella pertussis infections annually in the united states estimated by serologic surveys. reasons for the resurgence and current prevalenc ...	2015	26337739
shotgun proteome analysis of bordetella pertussis reveals a distinct influence of iron availability on the bacterial metabolism, virulence, and defense response.	one of the mechanisms involved in host immunity is the limitation of iron accessibility to pathogens, which in turn provokes the corresponding physiological adaptation of pathogens. this study reports a gel-free nanolc-ms/ms-based comparative proteome analysis of bordetella pertussis grown under iron-excess and iron-depleted conditions. out of the 926 proteins covered 98 displayed a shift in their abundance in response to low iron availability. forty-seven of them were found to be increased in l ...	2015	25755163
uptake of a government-funded pertussis-containing booster vaccination program for parents of new babies in victoria, australia.	an epidemic of bordetella pertussis in victoria, australia, led to the implementation of a government-funded vaccination program for parents of new babies. the rationale was to protect unimmunised infants from infection by vaccinating parents with a pertussis-containing vaccine. this is known as cocooning.	2015	25728321