Publications

primary and opportunistic pathogens associated with meningitis in adults in bangui, central african republic, in relation to human immunodeficiency virus serostatus.	to determine the causative organisms and characteristics of patients presenting with meningitis in bangui in order to provide guidance to physicians for case management.	2006	16473538
impaired response to listeria in h2-m3-deficient mice reveals a nonredundant role of mhc class ib-specific t cells in host defense.	the major histocompatibility complex (mhc) class ib molecule h2-m3 primes the rapid expansion of cd8+ t cells by presenting n-formylated bacterial peptides. however, the significance of h2-m3-restricted t cells in host defense against bacteria is unclear. we generated h2-m3-deficient mice to investigate the role of h2-m3 in immunity against listeria monocytogenes (lm), a model intracellular bacterial pathogen. h2-m3-deficient mice are impaired in early bacterial clearance during primary infectio ...	2006	16476767
monoclonal antibodies binding to the cell surface of listeria monocytogenes serotype 4b.	serotype 4b strains of the food-borne pathogen listeria monocytogenes are responsible for a large portion of sporadic listeric infections and all major food-borne listeriosis outbreaks in humans. hybridomas were produced from three fusions with lymphocytes of nd4 mice immunized either with the insoluble antigens of l. monocytogenes serotype 4b or with formalin-killed bacterial cells and screened for monoclonal antibodies (mabs) reactive to l. monocytogenes serotype 4b. a set of 35 mabs was ident ...	2006	16476793
occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in spain.	to survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions of fresh marine fish.	2006	16478492
evidence of an antilisterial factor induced by wounding of iceberg lettuce tissues.	to examine the influence of wound-associated reactions in cut iceberg lettuce (lactuca sativa l.) tissues on the fate of listeria monocytogenes.	2006	16478519
[listeria monocytogenes meningitis complicated after operation for thoracic aortic aneurysm].	the patient was a 71-year-old male. he was admitted as an emergency to our hospital because of impending rupture of thoracic aortic aneurysm with anterior chest pain and hoarseness. the aneurysm was saccular type and located in the lesser curvature of aortic arch. emergency operation was performed. entry of the aneurysm was excluded by including artificial graft on the deep hypothermic circulatory arrest. postoperative course was uneventful, but sudden depression of consciousness level and pyrex ...	2006	16482907
characterization of the bifunctional glycosyltransferase/acyltransferase penicillin-binding protein 4 of listeria monocytogenes.	multimodular penicillin-binding proteins (pbps) are essential enzymes responsible for bacterial cell wall peptidoglycan (pg) assembly. their glycosyltransferase activity catalyzes glycan chain elongation from lipid ii substrate (undecaprenyl-pyrophosphoryl-n-acetylglucosamine-n-acetylmuramic acid-pentapeptide), and their transpeptidase activity catalyzes cross-linking between peptides carried by two adjacent glycan chains. listeria monocytogenes is a food-borne pathogen which exerts its virulenc ...	2006	16484198
mammalian peptidoglycan recognition protein tagl inhibits listeria monocytogenes invasion into epithelial cells.	peptidoglycan recognition proteins are a family of evolutionary conserved proteins that play a basic role in the innate immunity of insects, but their role in the immunity of mammals remains unclear. to elucidate its functions, a mouse member of the peptidoglycan recognition proteins family, tagl, was stably expressed in colon adenocarcinoma ht29 cells, and its effect on the invasion and intracellular growth of the enteroinvasive pathogenic bacterium listeria monocytogenes was assessed. the expr ...	2006	16487310
a combination of diagnostic tools for rapid screening of ovine listeriosis.	a combined serological and pcr method for the detection of listeria monocytogenes infection in symptomatic and asymptomatic ovine flocks was evaluated. seventy-eight milk samples and 157 serum samples were analysed using a l. monocytogenes pcr detection kit and an anti-listeriolysin o igg immunoassay kit. the combined use of these commercial kits allowed a rapid and effective detection of l. monocytogenes infection in both the early stage, before seroconversion, and in a later phase, even after ...	2006	16487553
effect of environmental parameters (temperature, ph and a(w)) on the individual cell lag phase and generation time of listeria monocytogenes.	the effect of the individual environmental factors temperature (2-30 degrees c), ph (4.4-7.4) and a(w) (0.947-0.995) as well as the combinations of these factors on the individual cell lag phase and the generation time of listeria monocytogenes was investigated. individual cells were isolated using a serial dilution protocol in microtiter plates, and subsequent growth was investigated by optical density (od) measurements at 600 nm. about 100 replicates were made for each set of environmental con ...	2006	16488043
adsorption of a biosurfactant on surfaces to enhance the disinfection of surfaces contaminated with listeria monocytogenes.	the effects of sodium hypochlorite (naocl) and peracetic acid/hydrogen peroxide (pah) on the inactivation of adherent listeria monocytogenes lo28 cells were examined. the surfaces tested were stainless steel and polytetrafluoroethylene (ptfe) conditioned or not with an anionic biosurfactant produced by pseudomonas fluorescens. dilution-neutralization methods were used to assess the effectiveness of sanitizer solutions on planktonic and adherent cells. tests were performed on l. monocytogenes cul ...	2006	16488496
a role for cortactin in listeria monocytogenes invasion of nih 3t3 cells, but not in its intracellular motility.	cortactin is an f-actin binding protein that binds to the arp2/3 complex, stimulates its actin nucleation activity, and inhibits actin filament debranching. using rna interference directed against cortactin, we explored the importance of cortactin for several processes involving dynamic actin assembly. silencing cortactin expression was efficiently achieved in hela and nih 3t3 cells, with less than 5% of cortactin expression in sirna-treated cells. surprisingly, endocytosis in hela and nih 3t3 c ...	2006	16489553
[neonatal listeriosis].	we present the case of a male with a neonatal listeria monocytogenes infection. its evolution was favorable with intravenous ampicillin and gentamicin. listeriosis is an infrequent cause of neonatal pustulosis. the infection is acquired from the mother after bacteremia with few symptoms (early-onset forms) or while passing through an infected birth canal (late-onset forms). a cytological study and the quick stain technique make fast diagnosis of potentially serious neonatal pustuloses possible.	2006	16540055
concentrations and tracking of listeria monocytogenes strains in a seafood-processing environment using a most-probable-number enrichment procedure and randomly amplified polymorphic dna analysis.	concentrations of environmental microflora and listeria monocytogenes were monitored at multiple environmental locations within a seafood-processing facility over the course of 6 months. concentrations of l. monocytogenes were determined using a most-probable-number (mpn) enrichment procedure. two floor drains had persistent low concentrations of l. monocytogenes (0.03 to >1,100 mpn/cm2). in comparison, concentrations of the other organisms in the drain were much higher (heterotrophic plate coun ...	2006	16541676
influence of different histories of the inoculum on lag phase and growth of listeria monocytogenes in meat models.	the aim of this study was to determine the effect of history of inoculum and preservatives on the lag phase and growth rate of listeria monocytogenes strains in meat products packaged under modified atmosphere conditions. inocula with different histories were added to meat models, and growth rate and lag phase of two strains of l. monocytogenes were measured at 5 and 10 degrees c. the meat model stored at 10 degrees c contained sodium lactate, but the model stored at 5 degrees c did not. the fiv ...	2006	16541682
inactivation of microorganisms in milk and apple cider treated with ultrasound.	nonthermal technologies are emerging as promising alternatives to heat treatment for food processing. ultrasound, defined as sound waves with a frequency greater than 20 khz, has proven bactericidal effects, especially when combined with other microbial-reduction strategies such as mild heating. in this study, ultrasound treatment (sonifier probe at 20 khz, 100% power level, 150 w acoustic power, 118 w/cm2 acoustic intensity) with or without the effect of mild heat (57 degrees c) was effective a ...	2006	16541685
thermal resistance parameters for pathogens in white grape juice concentrate.	the heat resistance of escherichia coli o157:h7, salmonella, and listeria monocytogenes that were in stationary phase, had been exposed to high osmotic pressure, or were acid adapted was evaluated in white grape juice concentrate (58 degrees brix, ph 3.3). the most heat-resistant cells of all three pathogens were those exposed to high osmotic pressure or in stationary phase. unlike in single-strength juices, in concentrate the acid-adapted cells for all three pathogens were less heat resistant t ...	2006	16541686
electron beam and gamma irradiation effectively reduce listeria monocytogenes populations on chopped romaine lettuce.	fresh, chopped romaine lettuce contaminated with a seven-strain cocktail of listeria monocytogenes (in a solution containing approximately 10(8) organisms per ml) that had attained a level of contamination of between 7 and 8 log cfu/g was packaged in 15-g samples. the lettuce was irradiated with a co60 source at 1.15 or 0.51 kgy and then stored at 4 degrees c. in addition, samples contaminated with isolated strains 16397, 0733, and 1992 were subjected to either electron beam irradiation at doses ...	2006	16541687
inactivation of escherichia coli o157:h7, salmonella typhimurium dt104, and listeria monocytogenes on inoculated alfalfa seeds with a fatty acid-based sanitizer.	alfalfa seeds were inoculated with a three-strain cocktail of escherichia coli o157:h7, salmonella enterica subsp. enterica serovar typhimurium dt104, or listeria monocytogenes by immersion to contain approximately 6 to 8 log cfu/g and then treated with a fatty acid-based sanitizer containing 250 ppm of peroxyacid, 1,000 ppm of caprylic and capric acids (emery 658), 1,000 ppm of lactic acid, and 500 ppm of glycerol monolaurate at a reference concentration of 1x. inoculated seeds were immersed at ...	2006	16541689
transfer of listeria monocytogenes during mechanical slicing of turkey breast, bologna, and salami.	a commercial delicatessen slicer was used as the vector for sequential quantitative transfer of listeria monocytogenes (i) from an inoculated slicer blade (approximately 10(8), 10(5), or 10(3) cfu per blade) to 30 slices of uninoculated delicatessen turkey, bologna, and salami, (ii) from inoculated product (approximately 10(8) cfu/cm2) to the slicer, and (iii) from inoculated product (10(8), 10(5), or 10(3) cfu/cm2) to 30 slices of uninoculated product via the slicer blade. cutting force and pro ...	2006	16541694
effect of fat content on infection by listeria monocytogenes in a mouse model.	an estimated 2,500 cases of listeriosis occur annually in the united states. listeriosis is particularly severe among pregnant women and immunocompromised individuals. little is known regarding the effect of the food matrix on the ability of l. monocytogenes to survive in the gastrointestinal tract and cause systemic infection. mice were inoculated with various doses of l. monocytogenes in skim milk, half & half, or whipping cream to determine whether differences in milk fat content influence th ...	2006	16541700
impact of the population of spoilage microflora on the growth of listeria monocytogenes on frankfurters.	approximately 100 cfu/cm2 of a five-strain mixture of listeria monocytogenes was coinoculated onto frankfurters with three different concentrations (10(2), 10(4), and 106 cfu/cm2) of an undefined spoilage microflora derived from commercial frankfurters. the frankfurters were vacuum packaged and stored at 10 degrees c for up to 48 days. the populations of l. monocytogenes, aerobic mesophilic bacteria, lactic acid bacteria, and enterobacteriaceae were determined at various time intervals during st ...	2006	16541704
cerebral listeriosis in an adult freiberger gelding.	listeria monocytogenes infection, although rare in horses, can lead to septicaemia, gastroenteritis and abortion or stillborn foals. equine cerebral listeriosis has been reported, but only in newborn animals. this report describes a freiberger gelding with severe neuronal symptoms of sudden onset. the animal collapsed within 24 h and was humanely killed. necropsy revealed multiple small brown to reddish foci within the brain stem and pons. histopathology demonstrated multifocal suppurative menin ...	2006	16542674
[molecular epidemiology of listeria monocytogenes infections in a health district of madrid in a 3-year period (2001-2003)].	during 2003, an increase in the number of cases of listeriosis was observed in a tertiary hospital in madrid. the objectives of this study were to review the clinical characteristics of the cases diagnosed from 2001 to 2003 and to investigate clonal relationships among the clinical isolates of listeria monocytogenes.	2006	16545315
distinct roles of tlr2 and the adaptor asc in il-1beta/il-18 secretion in response to listeria monocytogenes.	apoptosis-associated speck-like protein containing a c-terminal caspase recruitment domain (asc) is an adaptor molecule that has recently been implicated in the activation of caspase-1. we have studied the role of asc in the host defense against the intracellular pathogen listeria monocytogenes. asc was found to be essential for the secretion of il-1beta/il-18, but dispensable for il-6, tnf-alpha, and ifn-beta production, in macrophages infected with listeria. activation of caspase-1 was abolish ...	2006	16547271
chronic-contained rupture of an infected aneurysm of the abdominal aorta due to listeria monocytogenes.	we report a case of chronic-contained rupture of an infected aneurysm of the abdominal aorta, from which listeria monocytogenes was cultured. the diagnosis of rupture and retroperitoneal mass was made by computed tomography, whereas fdg -pet diagnosed vessel wall inflammation. the infectious nature only became apparent at surgery.	2006	16547362
lymphocytes are detrimental during the early innate immune response against listeria monocytogenes.	mice deficient in lymphocytes are more resistant than normal mice to listeria monocytogenes infection during the early innate immune response. this paradox remains unresolved: lymphocytes are required for sterilizing immunity, but their presence during the early stage of the infection is not an asset and may even be detrimental. we found that lymphocyte-deficient mice, which showed limited apoptosis in infected organs, were resistant during the first four days of infection but became susceptible ...	2006	16549598
directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen.	we have developed a vaccine strategy for generating an attenuated strain of an intracellular bacterial pathogen that, after uptake by professional antigen-presenting cells, does not replicate intracellularly and is readily killed. however, after degradation of the vaccine strain within the phagolysosome, target antigens are released into the cytosol for endogenous processing and presentation for stimulation of cd8(+) effector t cells. applying this strategy to the model intracellular pathogen li ...	2006	16549792
[meningitis due to listeria monocytogenes after spinal anesthesia].		2006	16553352
effect of a bacteriocin-activated polythene film on listeria monocytogenes as evaluated by viable staining and epifluorescence microscopy.	to evaluate the effect of a bacteriocin-activated polythene film on resting and growing populations of listeria monocytogenes.	2006	16553731
a newly developed assay to study the minimum inhibitory concentration of satureja spinosa essential oil.	the minimum inhibitory concentration (mic) of satureja spinosa essential oil against staphylococcus aureus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica, salmonella serovar enteritidis pt4 and bacillus cereus was comparatively assessed with an established optical density method as well as a novel impedimetric method.	2006	16553733
single cell variability of l. monocytogenes grown on liver pâté and cooked ham at 7 degrees c: comparing challenge test data to predictive simulations.	the variability in growth between individual listeria monocytogenes cells was investigated on liver pâté and cooked ham. these results were compared to monte carlo simulations based on data collected previously in broths (francois et al., submitted for publication).	2006	16553736
associated roles of hemolysin and p60 protein for the intracellular growth of bacillus subtilis.	hemolysin expressing bacillus subtilis strain (b. subtilis ble/hla) was used as a carrier for listerial protein p60 to study the impact of this protein on bacterial virulence independent of other gene products of listeria monocytogenes. bacillus subtilis ble/hlya exhibited longer cell chains than b. subtilis ble/hlya/iap. recombinant bacillus strains are able to adhere to the mouse macrophage-like j774 and human epithelial-like int407 cell lines. the bacterial number of b. subtilis ble/hlya/iap ...	2006	16553805
characterization of ers, a prfa-like regulator of enterococcus faecalis.	we have identified a transcriptional regulator, named ers (for enterococcal regulator of survival), of enterococcus faecalis, an important opportunistic bacterium commonly recovered from hospitalized patients. ers is a member of the crp/fnr family and is 69% similar to srv, a prfa-like regulator of streptococcus pyogenes implicated in virulence, and is the e. faecalis protein most closely related to prfa, a positive regulator of virulence genes in listeria monocytogenes. in an in vivo-in vitro m ...	2006	16553815
detection of escherichia coli o157:h7, salmonella spp., staphylococcus aureus and listeria monocytogenes in kimchi by multiplex polymerase chain reaction (mpcr).	we developed an mpcr assay for the simultaneous detection, in one tube, of escherichia coli o157:h7, salmonella spp., staphylococcus aureus and listeria monocytogenes using species-specific primers. the mpcr employed the e. coli o157:h7 specific primer stx2a, salmonella spp. specific primer its, s. aureus specific primer cap8a-b and l. monocytogenes specific primer hly. amplification with these primers produced products of 553, 312, 405 and 210 bp, respectively. all pcr products were easily dete ...	2006	16554723
essential role of tnf family molecule light as a cytokine in the pathogenesis of hepatitis.	light is an important costimulatory molecule for t cell immunity. recent studies have further implicated its role in innate immunity and inflammatory diseases, but its cellular and molecular mechanisms remain elusive. we report here that light is upregulated and functions as a proinflammatory cytokine in 2 independent experimental hepatitis models, induced by concanavalin a and listeria monocytogenes. molecular mutagenesis studies suggest that soluble light protein produced by cleavage from the ...	2006	16557300
acute bacterial meningitis in qatar.	to study the changes in the epidemiology, clinical and bacteriological profiles of bacterial meningitis in the era of the haemophilus influenzae type b (hib)vaccine and pneumococcus resistance.	2006	16501676
[serovars of listeria monocytogenes and related species isolated from human clinical specimens].	using phenotype techniques, characterization was realized of species and serovars of 255 strains of listeria isolated from human material: 220-86.3% were from patients with possible invasive disease, while 35-13.7% were from colonized healthy individuals. the strains were collected in several regions of brazil from 1969-2000. in individuals aged 0-10 or 41-60 years old listeria monocytogenes was isolated more often in cerebral spinal fluid than in blood cultures, including samples from renal tra ...	2006	16501763
microbiological quality of randomly selected ready-to-eat foods sampled between 2003 and 2005 in wales, uk.	since 1995, the publicly funded ready-to-eat food sampling and examination activities in wales have been coordinated and structured, using a novel approach for the identification of samples and premises. the latest set of data from this surveillance system reports the results from 3391 ready-to-eat foods sampled between november 2003 and march 2005. during this seventeen-month period all samples were examined for aerobic colony count, escherichia coli, listeria spp., bacillus cereus, salmonella, ...	2006	16503065
lentinan has a stimulatory effect on innate and adaptive immunity against murine listeria monocytogenes infection.	lentinan, a (1-3)-beta glucan from lentinus edodes, is licensed as an immunostimulatory drug. we tested the effect of lentinan in the well-established model system of the murine listeria monocytogenes infection. pre-treatment of bone marrow macrophages and dendritic cells with lentinan resulted in increased production of tnf-alpha and il-12 after l. monocytogenes infection in vitro. after lentinan treatment bone marrow macrophages showed increased no-production and enhanced cytotoxic activity ag ...	2006	16504933
[zoonotic importance of selected species of gram-positive bacteria.]	zoonoses continue to play a signicant role in both human and veterinary medicine. their eradication is difficult and some authors are convinced of their growing importance. among the pathogens of infectious diseases transmissible from animals to man are several gram-positive bacteria. the authors focus on three agents that present a risk in three different areas-alimentary infections (listeria monocytogenes), professional infections (streptococcus suis) and infections of breeders of so-called pe ...	2006	16508905
infection with listeria monocytogenes as a probe for placental immunological function.	this chapter will describe the use of infection with listeria monocytogenes, a gram-positive intracellular bacterium, to study immunological responses in the placenta. this bacterium is chosen because it has a predilection for replication in the placenta. as such, it is a significant pathogen for pregnant women, being a major cause of fetal mortality and morbidity if appropriate public health precautions are not observed. furthermore, this bacterium has been a major tool for studying innate immu ...	2006	16512000
improved dna probe detection of listeria monocytogenes in enrichment culture after physical-chemical fractionation.	bacterial detection in foods by nucleic acid probes is limited by microflora competition during selective enrichment. probe target concentration by extraction and fractionation of enrichments may diminish this limitation. the 1-h accuprobe chemiluminescent culture identification test for listeria monocytogenes was used as a model. its high detection threshold provides a stringent challenge for evaluating enrichment work-up protocols. detection of l. monocytogenes, at 1-4 colony-forming units/g f ...	2006	16512244
probiotic lactobacillus casei activates innate immunity via nf-kappab and p38 map kinase signaling pathways.	probiotic bacteria are microorganisms that benefit the host through improvement of the balance of intestinal microflora and possibly by augmentation of host defense systems. we examined the mechanisms for the up-regulation of innate immune responses by a probiotic lactobacillus casei atcc27139, in vivo. using mouse models of systemic listeria monocytogenes infection and metha fibrosarcoma tumorigenesis in combination with balb/c and scid mice, we found that parenteral administration of l. casei ...	2006	16513392
colon cancer cells: pro-invasive signalling.	colon cancer results from erroneous renewal of the enteric epithelium. mutations in stem cells, or their proliferative progenitors, cause accumulation of cells that invade into the stroma and continue to divide rather than migrating on top of the basement membrane prior to entering into apoptosis. many of these changes in invasive activity appear to be related to the invasion-suppressor role of e-cadherin. we have also investigated listeria monocytogenes and other enteric bacteria, since these b ...	2006	16513408
genetic and phenotypic characterization of listeria monocytogenes lineage iii.	listeria monocytogenes has been previously grouped into three evolutionary groups, termed lineages i, ii and iii. while lineages i and ii are commonly isolated from various sources, lineage iii isolates are rare and have several atypical and unique phenotypic characteristics. relative to their prevalence in other sources, lineage iii strains are overrepresented among isolates from food-production animals, and underrepresented among isolates from human clinical cases and foods. this work describe ...	2006	16514149
[infliximab therapy in crohn's disease complicated by listeria monocytogenes meningoencephalitis].		2006	16514404
comparison of two chromogenic media for the detection of listeria monocytogenes with the plating media recommended by en/din 11290-1.	the efficacy of two selective chromogenic culture media, agar listeria ottaviani and agosti (aloa) and rapid' l. mono for the detection of listeria monocytogenes in food, was compared with that of an official culture method according to the en/din 11290-01 and -02 protocols [corresponding to the section 35 lmbg (german food act) method]. a total of 310 pre-packed ready-to-eat food samples (100 of graved and cold smoked salmon, 130 of different raw and cooked sausages and 80 of delicatessen and m ...	2006	16515816
heterologous expression of betl, a betaine uptake system, enhances the stress tolerance of lactobacillus salivarius ucc118.	given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. the nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system betl in the probiotic strain lactobacillus salivarius ucc118. following nisin induction, strains expressing betl exhibited a significant increase in resistance to several stresses, ...	2006	16517668
tolerance of listeria monocytogenes to cell envelope-acting antimicrobial agents is dependent on sigb.	mutation of sigb impairs the ability of listeria monocytogenes to grow in sublethal levels, and to survive in lethal concentrations, of the bacteriocins nisin and lacticin 3147 and the antibiotics ampicillin and penicillin g. sigb may therefore represent an attractive target for the development of new control and treatment strategies for this important pathogen.	2006	16517678
cutting edge: the direct action of type i ifn on cd4 t cells is critical for sustaining clonal expansion in response to a viral but not a bacterial infection.	the action of type i ifn (ifn-i) on apcs is well studied, but their direct effect on cd4 t cells is unclear. to address this, we transferred ifn-i receptor-deficient (ifn-ir(0)) and -sufficient (wild-type, wt) tcr-transgenic cd4 t cells into wt mice and analyzed their response to immunization. in response to lymphocytic choriomeningitis virus immunization, wt cd4 t cells expanded approximately 100-fold, whereas ifn-ir(0) cd4 t cells expanded <10-fold. however, both wt and ifn-ir(0) cd4 t cells e ...	2006	16517698
neonatal septicemia in high risk babies in south-eastern nigeria.	septicemia is one of the major causes of morbidity and mortality in the neonatal period and it often has a rapid and fulminant course.	2006	16519624
veterinary and medical aspects of abortion in danish sheep.	the danish sheep population totals around 144,000 animals, but little is known of the causes and prevalance of diseases. this study focuses on the causes of abortion in danish sheep. during one breeding season, aborted foetuses and stillbirths with signs of intrauterine death or malformation were submitted for laboratory examination from a population of 3,758 breeding ewes. samples from 24 incidents of abortion and 21 ewes delivering malformed lambs or lambs with ante partum decomposition were s ...	2006	16519752
invasiveness is a variable and heterogeneous phenotype in listeria monocytogenes serotype strains.	the ability of listeria monocytogenes to breach mucosal and endothelial barriers of the host during infection is a hallmark property mediated by the internalins (inl) a and b. we examined the invasive property of several l. monocytogenes strains representing 13 serotypes. we found that invasiveness is a heterogeneous phenotype amongst l. monocytogenes serotype strains. despite this, many of the poorly invasive and non-invasive strains of l. monocytogenes express internalins at levels comparable ...	2006	16527541
the ferritin-like protein frm is a target for the humoral immune response to listeria monocytogenes and is required for efficient bacterial survival.	the identity and role of listerial antigens recognized by antibodies following listerial infection is largely unknown. we identified the listerial ferritin-like protein frm as a target of the humoral response following infection of mice with pathogenic listeria monocytogenes. specific antibodies to frm are detected in antisera of mice infected with a pathogenic strain but not in antisera of mice infected with a non-pathogenic l. innocua strain. antibodies raised to purified listerial ferritin al ...	2006	16528570
[agglutination as screening test in routine diagnostic of listeriosis].	the persons with weakened immunity, newborn infants and pregnant women are very much sensitive on infection with bacteria listeria monocytogenes. listeria can readily invade the placenta and precipitate premature labor and fetal death. to confirm diagnosis is very difficult because listeriosis is disease very similar to the others acute diseases. serum threshold values above 1: 320 confirm think about listeriosis when the relevant clinical symptoms are present. of greater diagnostic relevance is ...	2006	16528925
genotypic and phenotypic heterogeneity in enterococcus isolates from batzos, a raw goat milk cheese.	this study investigated the genotypic and phenotypic diversity in 34 isolates of enterococci obtained during ripening of batzos cheese from raw goat milk and characterized phenotypically as enterococcus durans. rapd-pcr, plasmid profiling and pfge were used to study the genetic variability and distinguish closely related isolates. species recognition by means of rapd-pcr was in agreement with the phenotypic identification for 29 strains. one strain was characterized as lactococcus lactis subsp. ...	2006	16529834
[functional activity of mouse peritoneal macrophages contaminated with gram-positive bacteria].	the analysis of the functional and enzymatic activity of mouse peritoneal macrophages contaminated with staphylococcus aureus and listeria monocytogenes virulent strains is presented. the low bactericidal and digestive activity of these cells with respect to the above-mentioned microorganisms was determined. in this study a decrease in the activity of plasmatic membrane enzymes (5'-nucleotidase and atp-ase) of macrophages contaminated with s. aureus and l. monocytogenes was observed, which was i ...	2006	16532641
role of lung surfactant in phagocytic clearance of apoptotic cells by macrophages in rats.	two of the common features of inflammatory lung diseases are the increased production of pulmonary surfactant and the induction of lung cell apoptosis. however, the relationship between these two events has not been addressed. to investigate the role of surfactant in pulmonary inflammation and apoptosis, we instilled natural lung surfactant (survanta) (1.6-12.5 mg) into the rat lungs and determined the number of alveolar macrophages (ams) and apoptotic lung cells. high-dose treatments of survant ...	2006	16534500
[neonatal infection with listeria monocytogenes: rare, but serious].	between 1993 and 2003, three infants, two girls and a boy, were found to have an invasive infection with listeria monocytogenes. they received intensive care including respiratory and circulatory support, antibiotics, and treatment of the neurological complications when possible. one of the girls survived without sequelae but the other two infants died in the neonatal period. in one of these two cases there was a clear clue to the source of the infection in the dietary history of the mother: she ...	2006	16440567
phosphorylation, ubiquitination and degradation of listeriolysin o in mammalian cells: role of the pest-like sequence.	listeria monocytogenes is a bacterial pathogen that grows within the cytosol of infected host cells. entry into the cytosol is largely mediated by a secreted bacterial cytolysin, listeriolysin o (llo). in order to prevent host cell damage, the pore-forming activity of llo is restricted to the phagosome. compartmentalization of llo requires a pest-like sequence; pest sequences can direct eukaryotic proteins for proteasomal degradation. here we test the hypothesis that llo's pest-like sequence com ...	2006	16441444
listeria monocytogenes in platelets: a case report.	efforts to reduce bacterial contamination in platelets (plts) have led to implementation of tests for bacterial detection before product release. although relatively rare as a human pathogen, listeria monocytogenes often causes serious illness and has a case-fatality rate of 20 percent.	2006	16441611
interferon-producing killer dendritic cells provide a link between innate and adaptive immunity.	natural killer (nk) cells and dendritic cells (dcs) are, respectively, central components of innate and adaptive immune responses. we describe here a third dc lineage, termed interferon-producing killer dcs (ikdcs), distinct from conventional dcs and plasmacytoid dcs and with the molecular expression profile of both nk cells and dcs. they produce substantial amounts of type i interferons (ifn) and interleukin (il)-12 or ifn-gamma, depending on activation stimuli. upon stimulation with cpg oligod ...	2006	16444266
allelic exchange and site-directed mutagenesis probe the contribution of acta amino-acid variability to phosphorylation and virulence-associated phenotypes among listeria monocytogenes strains.	to test the hypothesis that acta allelic variation contributes to virulence differences among listeria monocytogenes strains, cell-to-cell spread and intracellular acta phosphorylation patterns were characterized for 14 wild-type isolates and selected isogenic mutants. our data show that (i) while acta allelic variation is not responsible for enhanced cell-to-cell spread observed in epidemic clone i strains, acta allelic variation may contribute to reduced plaque size observed in some isolates, ...	2006	16445760
listeria monocytogenes invades the epithelial junctions at sites of cell extrusion.	listeria monocytogenes causes invasive disease by crossing the intestinal epithelial barrier. this process depends on the interaction between the bacterial surface protein internalin a and the host protein e-cadherin, located below the epithelial tight junctions at the lateral cell-to-cell contacts. we used polarized mdck cells as a model epithelium to determine how l. monocytogenes breaches the tight junctions to gain access to this basolateral receptor protein. we determined that l. monocytoge ...	2006	16446782
selective targeting of antitumor immune responses with engineered live-attenuated listeria monocytogenes.	improved immunization and ex vivo t-cell culture strategies can generate larger numbers and more potent tumor-specific effector cells than previously possible. nonetheless, the capacity of these cells to eliminate established tumors is limited by their ability to efficiently enter tumor-bearing organs and mediate their effector function. in the current study, we show that the administration of an engineered organ-homing microbe selectively targets tumor-specific immune responses to metastases wi ...	2006	16424046
cutting edge: a novel nonoxidative phagosomal mechanism exerted by cathepsin-d controls listeria monocytogenes intracellular growth.	deciphering how listeria monocytogenes exploits the host cell machinery to invade mammalian cells is a key issue in understanding the pathogenesis of this food-borne pathogen, which can cause diseases ranging from gastroenteritis to meningitis and abortion. in this study, we show that the lysosomal aspartyl-protease cathepsin-d (ctsd) is of considerable importance for nonoxidative listericidal defense mechanisms. we observed enhanced susceptibility to l. monocytogenes infection of fibroblasts an ...	2006	16424157
protein vaccines induce uncommitted il-2-secreting human and mouse cd4 t cells, whereas infections induce more ifn-gamma-secreting cells.	mouse and human cd4 t cells primed during an immune response may differentiate into effector phenotypes such as th1 (secreting ifn-gamma) or th2 (secreting il-4) that mediate effective immunity against different classes of pathogen. however, primed cd4 t cells can also remain uncommitted, secreting il-2 and chemokines, but not ifn-gamma or il-4. we now show that human cd4 t cells primed by protein vaccines mostly secreted il-2, but not ifn-gamma, whereas in the same individuals most cd4 t cells ...	2006	16424174
sigma b contributes to listeria monocytogenes gastrointestinal infection but not to systemic spread in the guinea pig infection model.	contributions of the alternative sigma factor sigmab to listeria monocytogenes infection were investigated using strains bearing null mutations in sigb, prfa, or inla or in selected inla or prfa promoter regions. the deltap4inla strain, which has a deletion in the sigmab-dependent p4inla promoter, and the deltasigb strain had significantly reduced invasion efficiencies relative to that of the wild-type strain in the caco-2 human colorectal epithelial cell line, while the invasion efficiency of a ...	2006	16428730
identification of novel gammadelta t-cell subsets following bacterial infection in the absence of vgamma1+ t cells: homeostatic control of gammadelta t-cell responses to pathogen infection by vgamma1+ t cells.	although gammadelta t cells are a common feature of many pathogen-induced immune responses, the factors that influence, promote, or regulate the response of individual gammadelta t-cell subsets to infection is unknown. here we show that in the absence of vgamma1+ t cells, novel subsets of gammadelta t cells, expressing t-cell receptor (tcr)-vgamma chains that normally define tcrgammadelta+ dendritic epidermal t cells (detcs) (vgamma5+), intestinal intraepithelial lymphocytes (iiels) (vgamma7+), ...	2006	16428757
intracellular gene expression profile of listeria monocytogenes.	listeria monocytogenes is a gram-positive, food-borne microorganism responsible for invasive infections with a high overall mortality. l. monocytogenes is among the very few microorganisms that can induce uptake into the host cell and subsequently enter the host cell cytosol by breaching the vacuolar membrane. we infected the murine macrophage cell line p388d1 with l. monocytogenes strain egd-e and examined the gene expression profile of l. monocytogenes inside the vacuolar and cytosolic environ ...	2006	16428782
development of a magnetic capture hybridization-pcr assay for listeria monocytogenes direct detection in milk samples.	a rapid and sensitive method for listeria monocytogenes direct detection from milk was developed. it is based on a magnetic capture hybridization procedure for selective dna purification, followed by pcr identification. a comparison with two similar commercial systems from dynal (dynabeads) was carried out.	2006	16430514
iron acquisition systems for ferric hydroxamates, haemin and haemoglobin in listeria monocytogenes.	listeria monocytogenes is a gram-positive bacterium that causes severe opportunistic infections in humans and animals. we biochemically characterized, for the first time, the iron uptake processes of this facultative intracellular pathogen, and identified the genetic loci encoding two of its membrane iron transporters. strain egd-e used iron complexes of hydroxamates (ferrichrome and ferrichrome a, ferrioxamine b), catecholates (ferric enterobactin, ferric corynebactin) and eukaryotic binding pr ...	2006	16430693
mechanism of polarization of listeria monocytogenes surface protein acta.	the polar distribution of the acta protein on the surface of the gram-positive intracellular bacterial pathogen, listeria monocytogenes, is required for bacterial actin-based motility and successful infection. acta spans both the bacterial membrane and the peptidoglycan cell wall. we have directly examined the de novo acta polarization process in vitro by using an acta-rfp (red fluorescent protein) fusion. after induction of expression, acta initially appeared at distinct sites along the sides o ...	2006	16430699
actin-dependent movement of bacterial pathogens.	listeria, rickettsia, burkholderia, shigella and mycobacterium species subvert cellular actin dynamics to facilitate their movement within the host cytosol and to infect neighbouring cells while evading host immune surveillance and promoting their intracellular survival. 'attaching and effacing' escherichia coli do not enter host cells but attach intimately to the cell surface, inducing motile actin-rich pedestals, the function of which is currently unclear. the molecular basis of actin-based mo ...	2006	16415925
hot water postprocess pasteurization of cook-in-bag turkey breast treated with and without potassium lactate and sodium diacetate and acidified sodium chlorite for control of listeria monocytogenes.	surface pasteurization and food-grade chemicals were evaluated for the ability to control listeriae postprocess on cook-in-bag turkey breasts (cibtb). individual cibtb were obtained directly from a commercial manufacturer and surface inoculated (20 ml) with a five-strain cocktail (ca. 7.0 log) of listeria innocua. in each of two trials, the product was showered or submerged for up to 9 min with water heated to 190, 197, or 205 degrees f (ca. 87.8, 91.7, or 96.1 degrees c) in a commercial pasteur ...	2006	16416899
combining organic acid treatment with steam pasteurization to eliminate listeria monocytogenes on fully cooked frankfurters.	an organic acid solution of 2% acetic, 1% lactic, 0.1% propionic, and 0.1% benzoic acids was combined with steam surface pasteurization to treat frankfurters during vacuum packaging to eliminate potential postcook contamination with listeria monocytogenes. the thermal lethality of l. monocytogenes from steam was evaluated at an inoculation concentration of 1 to 6 log cfu/cm2. about 3-log reductions of l. monocytogenes were achieved when frankfurters were treated by steam for 1.5 s. combining org ...	2006	16416900
post process control of listeria monocytogenes on commercial frankfurters formulated with and without antimicrobials and stored at 10 degrees c.	the antilisterial effect of postprocess antimicrobial treatments on commercially manufactured frankfurters formulated with and without a 1.5% potassium lactate-0.05% sodium diacetate combination was evaluated. frankfurters were inoculated (ca. 3 to 4 log cfu/cm2) with 10-strain composite listeria monocytogenes cultures originating from different sources. the inocula evaluated were cells grown planktonically in tryptic soy broth plus 0.6% yeast extract (30 degrees c, 24 h) or in a smoked sausage ...	2006	16416901
a predictive model for the effect of temperature and predrying treatments in reducing listeria monocytogenes populations during drying of beef jerky.	the objective of this study was to model the effect of drying temperatures (52, 57, and 63 degrees c) and predrying treatments on the inactivation of listeria monocytogenes on beef jerky. before drying, beef slices were inoculated with a 10-strain composite of l. monocytogenes and then treated with the following: (i) nothing (c), (ii) traditional marinade (m), or (iii) dipping in 5% acetic acid solution for 10 min, followed by m (am). in addition, sequential stresses (exposure to 10% nacl, follo ...	2006	16416902
cross-contamination between processing equipment and deli meats by listeria monocytogenes.	contamination of luncheon meats by listeria monocytogenes has resulted in outbreaks of listeriosis and major product recalls. listeriae can survive on processing equipment such as meat slicers which serve as a potential contamination source. this study was conducted to determine (i) the dynamics of cross-contamination of l. monocytogenes from a commercial slicer and associated equipment onto sliced meat products, (ii) the influence of sample size on the efficacy of the bax-pcr and u.s. departmen ...	2006	16416903
molecular characterization of listeria monocytogenes from natural and urban environments.	characterization of 80 listeria monocytogenes isolates from urban and natural environments differentiated 7 and 26 ecori ribotypes, respectively. whereas the majority of isolates from the natural environment represented l. monocytogenes lineage ii (12 of 13 isolates), urban isolates grouped evenly into lineages i and ii (32 and 33 isolates, respectively) and included two lineage iii isolates. multilocus sequence typing of all natural isolates and a randomly selected subset of 30 urban isolates s ...	2006	16416906
sensitivities of foodborne pathogens to pressure changes.	eight foodborne pathogens were suspended in ultrahigh-temperature whole milk and treated at pressure levels of 0.1 to 690 mpa at 21.5 degrees c for 10 min. there was no clear trend in pressure resistance between gram-negative and gram-positive organisms. the order of the single strains tested, from most to least pressure sensitive, was vibrio parahaemolyticus < yersinia enterocolitica < listeria monocytogenes < salmonella enterica serovar typhimurium < s. enterica serovar enteritidis < escherich ...	2006	16416910
influence of antimicrobial compounds and modified atmosphere packaging on radiation sensitivity of listeria monocytogenes present in ready-to-use carrots (daucus carota).	radiosensitization of listeria monocytogenes was determined in the presence of trans-cinnamaldehyde, spanish oregano, winter savory, and chinese cinnamon on peeled minicarrots packed under air or under a modified atmosphere (60% o2, 30% co2, and 10% n2). samples were inoculated with l. monocytogenes hpb 2812 serovar 1/2a (106 cfu/g) and were coated separately with each active compound (0.5%, wt/wt) before being packaged under air or the modified atmosphere and irradiated at doses from 0.07 to 2. ...	2006	16416924
therapy of paraneoplastic pemphigus with rituximab: a case report and review of literature.	paraneoplastic pemphigus (pnp) is an autoimmune blistering disease with poor prognosis when associated with malignant neoplasm. we report the case of a patient with pnp associated with a cd20+ non-hodgkin follicular lymphoma who was treated with rituximab plus corticosteroids and short courses of cyclosporin. one and a half years after rituximab therapy, oral ulcerations had cleared and oral methylprednisolone was slowly tapered down without further recurrences. in the course of the disease, the ...	2006	16405612
multiple characterizations of listeria monocytogenes sensitive and insensitive variants to divergicin m35, a new pediocin-like bacteriocin.	divergicin m35 is a new class iia bacteriocin produced by carnobacterium divergicin m35. the bactericidal activity of this antimicrobial peptide was tested against a set of 11 strains of listeria monocytogenes isolated from food.	2006	16405682
partial characterization of enterocin mr99 from a corn silage isolate of enterococcus faecalis.	to assess the inhibitory activity on gram-positive and gram-negative bacteria of several species of enterococci recovered from a natural corn silage.	2006	16405692
evaluation of live-culture-producing lacticin 3147 as a treatment for the control of listeria monocytogenes on the surface of smear-ripened cheese.	a live lactococcus lactis culture, producing the two-component broad spectrum bacteriocin lacticin 3147, was assessed for ability to inhibit the food pathogen listeria monocytogenes on the surface of smear-ripened cheese.	2006	16405693
bacterial rna and small antiviral compounds activate caspase-1 through cryopyrin/nalp3.	missense mutations in the cias1 gene cause three autoinflammatory disorders: familial cold autoinflammatory syndrome, muckle-wells syndrome and neonatal-onset multiple-system inflammatory disease. cryopyrin (also called nalp3), the product of cias1, is a member of the nod-lrr protein family that has been linked to the activation of intracellular host defence signalling pathways. cryopyrin forms a multi-protein complex termed 'the inflammasome', which contains the apoptosis-associated speck-like ...	2006	16407888
cryopyrin activates the inflammasome in response to toxins and atp.	a crucial part of the innate immune response is the assembly of the inflammasome, a cytosolic complex of proteins that activates caspase-1 to process the proinflammatory cytokines interleukin (il)-1beta and il-18. the adaptor protein asc is essential for inflammasome function, binding directly to caspase-1 (refs 3, 4), but the triggers of this interaction are less clear. asc also interacts with the adaptor cryopyrin (also known as nalp3 or cias1). activating mutations in cryopyrin are associated ...	2006	16407890
invasion assay of listeria monocytogenes using vero and caco-2 cells.	the invasion ability of listeria monocytogenes into cultured cells has been used to evaluate its pathogenicity. in this study, invasive ability was investigated using vero and caco-2 cell lines. the form of invasion showed no morphological differences between both cell lines inoculated with l. monocytogenes l89-h2 or l96-23c1 strains when double fluorescence stained with rhodamine and fitc or with giemsa staining. recovery count and recovery rate of l. monocytogenes from vero cells was related t ...	2006	16410027
detection and quantification of listeria monocytogenes by 5'-nuclease polymerase chain reaction targeting the acta gene.	the aim of this study was to develop a 5'-nuclease polymerase chain reaction (pcr) for the rapid detection and quantification of listeria monocytogenes.	2006	16411913
ultrastructural studies on antimicrobial efficacy of thyme essential oils on listeria monocytogenes.	listeria monocytogenes has gained increasing attention as a pathogen of public health importance owing to large numbers of food-borne outbreaks of listeriosis. because of negative consumer perception of chemical preservatives, attention is shifting towards natural alternatives. particular interest has been focused on the potential application of plant essential oils. the objective of the present study was to determine ultrastructural changes brought about by essential oils from two types of thym ...	2006	16412677
recognition of cytosolic dna activates an irf3-dependent innate immune response.	nucleic acid recognition upon viral infection triggers type i interferon production. viral rna is detected by both endosomal, tlr-dependent and cytosolic, rig-i/mda5-dependent pathways. tlr9 is the only known sensor of foreign dna; it is unknown whether innate immune recognition of dna exists in the cytosol. here we present evidence that cytosolic dna activates a potent type i interferon response to the invasive bacterium listeria monocytogenes. the noninvasive legionella pneumophila triggers an ...	2006	16413926
presentation of self-antigens on mhc class ii molecules during dendritic cell maturation.	little is known about how dendritic cells (dcs) maintain a balance between tolerance and immunity for antigens synthesized by dcs themselves. using transgenic dcs expressing a model self-antigen, in vitro self-peptide-mhc class ii complex formation and presentation increased with dc maturation, as for exogenous antigens. in vivo, however, even 'immature' dcs isolated from steady-state lymph nodes expressed mhc at mature cell levels, although many were also cd86 low. adoptive transfer of naive sp ...	2006	16361313
subversion of cellular functions by listeria monocytogenes.	listeria monocytogenes is a gram-positive bacterium that is able to invade and multiply within eukaryotic cells. its intracellular life-cycle includes pathogen-induced phagocytosis, lysis of the phagocytic vacuole, movement in the cytoplasmic environment, and a cell-to-cell spread mechanism. many l. monocytogenes virulence factors have been studied in detail, certain of which subvert specific eukaryotic cell functions in order to favour infection. during entry, the invasion protein inla takes ad ...	2006	16362984
listeria monocytogenes activated p38 mapk and induced il-8 secretion in a nucleotide-binding oligomerization domain 1-dependent manner in endothelial cells.	nucleotide-binding oligomerization domain (nod) proteins serve as intracellular pattern recognition molecules recognizing peptidoglycans. to further examine intracellular immune recognition, we used listeria monocytogenes as an organism particularly amenable for studying innate immunity to intracellular pathogens. in contrast to wild-type l. monocytogenes, the nonpathogenic listeria innocua, or l. monocytogenes mutants lacking internalin b or listeriolysin o, poorly invaded host cells and escape ...	2006	16365441
il-15 regulates cd8+ t cell contraction during primary infection.	during the course of acute infection with an intracellular pathogen, ag-specific t cells proliferate in the expansion phase, and then most of the t cells die by apoptosis in the following contraction phase, but the few that survive become memory cells and persist for a long period of time. although il-15 is known to play an important role in long-term maintenance of memory cd8+ t cells, the potential roles of il-15 in cd8+ t cell contraction are not known. using an adoptive transfer system of ot ...	2006	16365444
carbon metabolism of intracellular bacteria.	bacterial metabolism has been studied intensively since the first observations of these 'animalcules' by leeuwenhoek and their isolation in pure cultures by pasteur. metabolic studies have traditionally focused on a small number of model organisms, primarily the gram negative bacillus escherichia coli, adapted to artificial culture conditions in the laboratory. comparatively little is known about the physiology and metabolism of wild microorganisms living in their natural habitats. for approxima ...	2006	16367862
cytolysin-dependent delay of vacuole maturation in macrophages infected with listeria monocytogenes.	the bacterial pathogen listeria monocytogenes (lm) evades the antimicrobial mechanisms of macrophages by escaping from vacuoles to the cytosol, through the action of the cytolysin listeriolysin o (llo). because of heterogeneities in the timing and efficiency of escape, important questions about the contributions of llo to lm vacuole identity and trafficking have been inaccessible. expression of cyan fluorescent protein (cfp)-labelled endocytic membrane markers in macrophages along with a yellow ...	2006	16367870
identification of the insulin-like growth factor ii receptor as a novel receptor for binding and invasion by listeria monocytogenes.	the gram-positive bacterium listeria monocytogenes causes a life-threatening disease known as listeriosis. the mechanism by which l. monocytogenes invades mammalian cells is not fully understood, but the processes involved may provide targets to prevent and treat listeriosis. here, for the first time, we have identified the insulin-like growth factor ii receptor (igfiir; also known as the cation-independent mannose 6-phosphate receptor (ci)m6pr or cd222) as a novel receptor for binding and invas ...	2006	16369013