Publications

comparison of initial yields of dna-to-protein crosslinks and single-strand breaks induced in cultured human cells by far- and near-ultraviolet light, blue light and x-rays.	the initial yields of dna-to-protein crosslinks (dpc) caused by ionizing and nonionizing radiations were compared, with emphasis upon values within the biological dose ranges (d0). induction of dpc in cold (0-0.5 degrees c) human p3 teratocarcinoma cells was measured by using alkaline elution techniques after exposure to monochromatic uvc (254 nm), uvb (313 nm), uva (365 and 405 nm), and blue light (434 nm). uvc and uvb light induced detectable numbers (about 100 dpc per cell per d0). monochroma ...	1991	1986263
relationship between body and brain temperature in traumatically brain-injured rodents.	recent work has shown that mild to moderate levels of hypothermia may profoundly reduce the histological and biochemical sequelae of cerebral ischemic injury. in the present study, the authors examined the effect of fluid-percussion injury on brain temperature in anesthetized rats and the effect of anesthesia on brain temperature in uninjured rats. the relationship between the brain, rectal, and temporalis muscle temperatures during normothermia, hypothermia, and hyperthermia was studied followi ...	1991	1993914
practical field epidemiology to investigate a cholera outbreak in a mozambican refugee camp in malawi, 1988.	of all populations affected by cholera, refugees are at particular risk of infection due to overcrowding and poor sanitation. between 15 march and 17 may 1988, 951 cases of cholera were registered at the cholera treatment centre in a mozambican refugee camp in malawi. the epidemic duration was 65 days. vibrio cholerae biotype e1 tor serotype inaba was isolated. to identify high-risk groups and potential risk of acquiring the disease, an epidemiologic investigation was conducted. the attack rate ...	1991	1995929
freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles.	freeze-substitution was performed on strains of escherichia coli, pasteurella multocida, campylobacter fetus, vibrio cholerae, pseudomonas aeruginosa, pseudomonas putida, aeromonas salmonicida, proteus mirabilis, haemophilus pleuropneumoniae, caulobacter crescentus, and leptothrix discophora with a substitution medium composed of 2% osmium tetroxide and 2% uranyl acetate in anhydrous acetone. a thick periplasmic gel ranging from 10.6 to 14.3 nm in width was displayed in e. coli k-12, k30, and hi ...	1991	1999383
toxr regulates the production of lipoproteins and the expression of serum resistance in vibrio cholerae.	the genes encoding three lipoproteins of vibrio cholerae were identified by a combination of dna sequence analysis and [3h]palmitate labeling of hybrid proteins encoded by tnphoa gene fusions. the expression of these three lipoproteins, taga, acfd, and tcpc, was controlled by toxr, the cholera toxin transcriptional activator. the involvement of other bacterial lipoproteins in conferring resistance to the bactericidal effects of complement prompted us to examine this possibility in v. cholerae. r ...	1991	2000374
direct evidence that ganglioside is an integral component of the thyrotropin receptor.	gangliosides were extracted from purified human and porcine thyrotropin (tsh) receptors (tsh-r) and were detected by probing with an 125i-labeled sialic acid-specific lectin, limax flavus agglutinin. gangliosides copurified with human and porcine tsh-r migrated between monosialoganglioside gm1 and disialoganglioside gd1a. ceramide glycanase digestion of the purified human tsh-r-associated glycolipid confirmed its ganglioside nature. it was resistant to vibrio cholerae sialidase, which digests al ...	1991	2000404
immunogenicity of two formulations of oral cholera vaccines in thai volunteers.	a formulation of oral vaccine consisting of vibrio cholerae lipopolysaccharides (lps), cell-bound haemagglutinin (cha) and procholeragenoid (p), namely vaccine a, was compared with another formulation, vaccine b, prepared from killed whole vibrios plus procholeragenoid on their immunogenicity and reactogenicity in thai male volunteers. volunteers were randomly allocated into three groups. the first two groups received orally three doses of vaccines a and b, respectively at 14-day intervals. volu ...	1991	2008802
purification and characterization of a secreted protease from the pathogenic marine bacterium vibrio anguillarum.	vibrio anguillarum is a pathogenic marine bacterium which causes the disease vibriosis in salmonid fish, which is characterized by a fatal hemorrhagic septicemia accompanied by massive tissue destruction. in this paper, the purification of the major caseinolytic extracellular protease from v. anguillarum is presented. the purification steps include ammonium sulfate precipitation, deae-sepharose chromatography, sephacryl s-200 chromatography, and deae high-pressure liquid chromatography. the puri ...	1991	2012804
synthetic peptides as the basis for vaccine design.		1991	2017193
identification of the genomic region determining serotype specificity of vibrio cholerae 01.	a 2.1-kb genomic region responsible for ogawa serotype specificity of vibrio cholerae 01 was identified by cosmid cloning and recombinant plasmid experiments. the plasmid carrying this region derived from ogawa type vibrio cholerae nih 41 coded for a specific protein of 27 kd, and was found to convert serotype specificity from inaba to ogawa when co-introduced into the escherichia coli cells harboring a cloned 20-kilobase genomic dna fragment of inaba type vibrio cholerae 35a3.	1991	2018510
production and characterization of monoclonal antibodies to vibrio cholerae soluble haemagglutinin.	monoclonal antibodies (mabs) against a protease of vibrio cholerae, the soluble haemagglutinin (sha), have been prepared and characterized with regard to their ability to inhibit different biological properties of sha and to protect against experimental v. cholerae infection. four fusion experiments yielded two specific immunoglobulin g1 mabs that reacted with sha produced by different v. cholerae o1 and non-o1 strains but that differed in their antigen-binding capacity. both mabs were capable o ...	1991	2018638
preferential reactivity of autoantibodies in murine lupus nzb mice to neuraminidase-treated monosialogangliosides on b cells of mouse spleen.	when analyzed by flow cytometry, reactivity of igm autoantibodies in sera from nzb mice to spleen b cells, but not to t cells, from balb/c mice was remarkably increased after treatment of the cells with vibrio cholerae neuraminidase. by tlc immunostaining with the antibodies, neither neutral nor acidic glycosphingolipids from both balb/c and nzb mouse spleens were found to be reactive, but after neuraminidase treatment of the tlc plate, prior to the immunostaining, three components became reacti ...	1991	2018978
the protective activity of tea against infection by vibrio cholerae o1.	extracts of black tea exhibited bactericidal activity against vibrio cholerae o1. the tea extract inhibited the haemolysin activity of v. cholerae o1, el tor and the morphological changes of chinese hamster ovary cells induced by cholera toxin. tea extract also reduced fluid accumulation induced by cholera toxin in sealed adult mice and by v. cholerae o1 in ligated intestinal loops of rabbits. these findings suggest that tea has protective activity against v. cholerae o1.	1991	2019547
cholera. small risk of cholera transmission by food imports.		1991	2021526
synthesis in vibrio cholerae and secretion of hepatitis b virus antigens fused to escherichia coli heat-labile enterotoxin subunit b.	a simple and effective electroporation method for the transformation of vibrio cholerae with nonmobilizable plasmids is described. expression plasmids directing the synthesis of fusion proteins with the subunit b of escherichia coli heat-labile enterotoxin b (lt-b) were transformed into nontoxinogenic v. cholerae vaccine strains. a protein consisting of two overlapping immunodominant antibody-binding sites of the hepatitis b virus (hbv) middle surface antigen fused to the c terminus of full-leng ...	1991	2022336
characterization of genetic variation and 3'-azido-3'-deoxythymidine- resistance mutations of human immunodeficiency virus by the rnase a mismatch cleavage method.	the rnase a mismatch cleavage method has been applied to the characterization of natural genetic variation of human immunodeficiency virus (hiv) from different geographical areas. the approach provides a rapid and simple assay for the analysis of differences in closely related viral isolates and allows the establishment of phylogenetic relationships between epidemiologically distinct viruses. our results show a broad clustering of circulating viruses according to their geographical distribution. ...	1991	2034672
tumor necrosis factor alpha selectively sensitizes human immunodeficiency virus-infected cells to heat and radiation.	we report here that infection of the human t-cell line hut-78 with human immunodeficiency virus (hiv) increases its sensitivity to heat and radiation toxicity. a possible explanation for this result may be the reduced expression of manganous superoxide dismutase (mnsod) in hiv-infected cells compared to uninfected cells. tumor necrosis factor alpha (tnf-alpha) further sensitizes hiv-infected cells but not uninfected cells to heat and radiation. this is consistent with the ability of tnf-alpha to ...	1991	2034677
enterotoxin-associated dna sequence homology between salmonella species and escherichia coli.	multiple hindiii-restriction fragments of salmonella typhimurium and salmonella typhi chromosomal dna exhibited homology with the heat-labile enterotoxin (lt1) gene of escherichia coli as determined by southern blot analysis. a 9.4 kb hindiii restriction fragment identified in s. typhimurium and s. typhi chromosomal dna reacted with both elta and eltb gene probes. however, the homology of the 9.4 kb dna fragment from these salmonella species was greater with eltb than elta. in addition, a synthe ...	1991	2037224
purification and characterization of a new heat-stable enterotoxin produced by vibrio cholerae non-o1 serogroup hakata.	the possible production of a heat-stable enterotoxin (vc-h-st) by vibrio cholerae non-o1 serogroup hakata was investigated, and the purified vc-h-st was characterized. it has a unique amino acid sequence, lidcceiccnpacfgcln. this sequence is quite similar to that of the heat-stable enterotoxin (nag-st) produced by v. cholerae non-o1 except for one amino acid (leucine) residue excess at the n terminus. other characteristics, including biological activity, are compatible with those of nag-st.	1991	2037379
field trial of oral cholera vaccines in bangladesh: serum vibriocidal and antitoxic antibodies as markers of the risk of cholera.	the relationship of serum vibriocidal (vc) and igg anti-cholera toxin (ct) antibodies to the risk of cholera was evaluated during the first year of follow-up of recipients of three oral doses of b subunit (bs)-whole-cell vaccine, whole-cell vaccine, or escherichia coli k12 strain placebo in bangladesh. acute sera from 121 cholera patients were compared with sera from 2592 contemporaneous community controls. each doubling of vc titer was associated, on average, with a 22%-47% reduction of cholera ...	1991	2037789
strain specific sensitivity to diethylnitrosamine-induced carcinogenesis is maintained in hepatocytes of c3h/hen in equilibrium with c57bl/6n chimeric mice.	the c3h/hen (c3h) and c57bl/6n (c57) mouse strains are known, respectively, for their high and low susceptibility to both spontaneous and chemically induced hepatocarcinogenesis. the present study was aimed at elucidating whether this difference is dependent on intrinsic features of the target hepatocytes or in the in vivo milieu and associated growth promoting factors to which the cells are exposed. c3h in equilibrium with c57 chimeric mice were produced and given injections of diethylnitrosami ...	1991	2040001
human immunodeficiency virus infection in pregnancy.	many aspects of hiv infection in pregnancy remain unclear. subsets at increased risk for perinatal transmission, adverse pregnancy outcome, and development of symptomatic hiv infection need to be identified. for instance, relative risks may be quite different in asymptomatic hiv infected patients with t4 lymphocyte counts greater than 200 cells per cubic millimeter compared to those with either symptoms of hiv infection or t4 cell counts less than 200 cells per cubic millimeter. at present, anti ...	1991	2040117
experimental liver cysts induced by 2-acetylaminofluorene.	liver cyst formation was studied serially in an experimental model in which rats were fed a diet containing 0.02% 2-acetylaminofluorene, a carcinogen, for 6 weeks, followed by a normal diet for 42 weeks. cysts appeared in the portal tracts at the 12th week, by which time the intrahepatic bile ducts had also proliferated and dilated. some of the dilated bile ducts were cavernous or multicystic and appeared to represent a transitional form between the bile duct dilation and cystic formation. after ...	1991	2040399
production of monoclonal antibodies against a hemagglutinin/protease of vibrio cholerae non-01.	two hybridoma cell lines producing monoclonal antibodies (mabs) against a hemagglutinin/protease (ha/p) from vibrio cholerae non-01 were produced and characterized. the two mabs contained the kappa light chain and were igg1 type. they similarly neutralized ha/p protease activity derived from both v. cholerae non-01 and v. cholerae 01, whereas they were unable to neutralize the hemagglutinating activity of ha/p, suggesting that the epitopes for protease and hemagglutination activities are differe ...	1991	2040430
abrogation of dominant glucose intolerance in sjl mice by a growth hormone transgene.	glucose tolerance was studied in transgenic mice (sjl x c57bl/6) expressing human gh under the control of a housekeeping promoter. parental sjl mice were found to harbour a dominant allele, termed here glid, determining glucose intolerance in pure-bred animals and in f1 hybrids with glucose-tolerant c57bl/6 mice. blood glucose levels in transgenic sjl x c57bl/6 hybrid mice were well controlled following glucose challenge, whereas non-transgenic hybrids failed to control their glucose adequately. ...	1991	2043241
detection of chlamydia trachomatis in first-void urine collected from men and women attending a venereal clinic.	cervical, urethral and first-void urine (fvu) specimens from 196 men and 245 women attending a venereal outpatient clinic were studied by culture and a commercial enzyme immunoassay (eia) (chlamydiazyme). confirmatory chlamydial testing by a direct fluorescence assay (dfa) (microtrak) was performed on the sediments of the positive eia samples from culture-negative patients. chlamydia trachomatis was isolated from 11% of the men and 12% of the women. of the women, 67% were positive in both sampli ...	1991	2043357
mind over muscle.		1991	2044511
sir frederick hobday memorial lecture. all wind and water: some progress in the study of equine gut motility.		1991	2044514
cardiac output during exercise in paraplegic subjects.	the purpose of this study was to measure the cardiac output using the co2 rebreathing method during submaximal and maximal arm cranking exercise in six male paraplegic subjects with a high level of spinal cord injury (hp). they were compared with eight able bodied subjects (ab) who were not trained in arm exercise. maximal o2 consumption (vo2max) was lower in hp (1.11.min, sd 0.1; 17.5 ml.min-1.kg-1, sd 4) than in ab (2.5 l.min-1, sd 0.6; 36.7 ml.min-1.kg, sd 10.7). maximal cardiac output was si ...	1991	2044535
effects of an endogenous ouabainlike compound on heart and aorta.	an endogenous ouabainlike compound (olc) has been purified from human plasma, and mass spectrometry has shown it to be indistinguishable from plant-derived ouabain. this human olc was tested for its effects on evoked tension in guinea pig left atria and aortic rings. the tissues were incubated at 37 degrees c in bicarbonate-buffered physiological salt solution gassed with 95% o2-5% co2. in atria stimulated electrically at 1 hz, 85 and 170 nm human olc increased peak active force to 177 +/- 15% a ...	1991	2045174
cloning and nucleotide sequence of the vibrio cholerae hemagglutinin/protease (ha/protease) gene and construction of an ha/protease-negative strain.	the structural gene hap for the extracellular hemagglutinin/protease (ha/protease) of vibrio cholerae was cloned and sequenced. the cloned dna fragment contained a 1,827-bp open reading frame potentially encoding a 609-amino-acid polypeptide. the deduced protein contains a putative signal sequence followed by a large propeptide. the extracellular ha/protease consists of 414 amino acids with a computed molecular weight of 46,700. in the absence of protease inhibitors, this is processed to the 32- ...	1991	2045361
duodenal rapeseed oil infusion in early and midlactation cows. 1. intestinal apparent digestibility of fatty acids and lipids.	rapeseed oil was infused continuously into the duodenum of lactating dairy cows. five separate trials were conducted with differences in amount of oil infused (1.0 to 1.5 kg/d), in lactation stage (early to late), and in basal diet (corn or grass silages). the effects of lecithin addition (9 g/d) also were studied. oil infusion did not affect the apparent digestibility of defatted organic matter. fatty acid, ether extract, and total lipid digestibilities in control cows were 62 to 82%, 72 to 76% ...	1991	2045558
vibrio cholerae produces a second enterotoxin, which affects intestinal tight junctions.	attenuated vibrio cholerae vaccine strains specifically mutated in genes encoding cholera toxin (ct) are still capable of causing mild to moderate diarrhea. culture supernatants of v. cholerae strains, both ct-positive and ct-negative, were examined in ussing chambers, and a toxin was found that increases the permeability of the small intestinal mucosa by affecting the structure of the intercellular tight junction, or zonula occludens. the activity of this toxin is reversible, heat-labile, sensi ...	1991	2052603
regulatory cascade controls virulence in vibrio cholerae.	expression of more than 17 virulence genes in vibrio cholerae is under the coordinate control of the toxr protein. toxr is a transmembrane protein that binds to and activates the promoter of the operon encoding cholera toxin. as yet, the ability of toxr to activate directly other genes in this regulon has not been demonstrated. we have cloned a gene called toxt from v. cholerae 569b; the toxt gene product, like toxr, can activate the ctx promoter in escherichia coli. in addition, expression of o ...	1991	2052618
risk of transmission of cholera by foods.		1991	2054288
evaluation of the antimicrobial activity of methylglyoxal bis(guanylhydrazone) analogues, the inhibitors for polyamine biosynthetic pathway.	metabolic and antiproliferative effects of methylglyoxal bis(butylamidinohydrazone) (mgbb) and methylglyoxal bis(cyclopentylamidinohydrazone) (mgbcp), inhibitors for polyamine biosynthetic pathway, on escherichia coli, shigella sonnei, aeromonas sobria, aeromonas hydrophila and vibrio cholerae were investigated. mgbb at the concentration of 100 mumol/l depleted intracellular putrescine and spermidine concentrations of e. coli to 25 and 20% of the controls, respectively, while mgbcp depressed the ...	1991	2055790
a method for studies of an el tor-associated antigen of vibrio cholerae o1.	a method for studying the biotype el tor associated mannose-sensitive haemagglutinin (msha) of v. cholerae o1 has been developed. by using crude msha adsorbed to chicken erythrocytes as solid phase antigen in an enzyme-linked immunosorbent assay (elisa), antisera against v. cholerae of the el tor biotype reacted in high titre with the msha-coated cells, whereas antisera against vibrios of the classical biotype did not bind significantly, i.e. in higher titre than pre-immune sera. the binding of ...	1991	2060759
cholera and cholera-like diarrhoeal diseases: why the differences in severity?	although vibrio cholera and several other enterotoxigenic bacteria such as the enterotoxigenic escherichia coli produce secretory diarrhoea by exactly the same mechanism, cholera is much more severe. it is proposed that this difference in severity may be because while the alkaline diarrhoeal fluid produced in these conditions is optimal for the growth of vibrio cholera, e. coli and other enterotoxigenic bacteria are inhibited.	1991	2062265
rubinstein-taybi syndrome with de novo reciprocal translocation t(2;16)(p13.3;p13.3).	we describe a girl with typical rubinstein-taybi syndrome with apparently balanced reciprocal translocation between chromosome 2 and 16. the patient has a condition characterized by mental retardation, typical facial manifestations, broad thumbs and first toes. cytogenetic studies of the patient showed a reciprocal translocation without visible deletion, karyotype: 46,xx, t(2;16)(p13.3;p13.3). her parents had normal chromosomes. these results suggest that the locus of the gene for the rubinstein ...	1991	2063911
epidermal cytokines and their roles in cutaneous wound healing.	cytokines are small proteins or glycoproteins which are synthesized and secreted by a variety of cell types. through binding to specific receptors on target cells, these hormone-like products regulate many normal cell activities, including growth and differentiation, migration and immune functions. within the epidermis, keratinocytes are the major source of cytokines along with melanocytes and langerhans cells. in response to a variety of injurious stimuli, including ultraviolet irradiation and ...	1991	2064935
effects of vibrio cholerae recombinant strains on rabbit ileum in vivo. enterotoxin production and myoelectric activity.	previous studies have identified the effects of vibrio cholerae and its enterotoxin, choleragen (ct a+b+), on the myoelectric activity of rabbit ileal loops in vivo. the response was defined as the migrating action potential complex, the single ring contraction that propels luminal contents aborad. in this study the same rabbit model is used to assess whether migrating action potential complex activity or fluid output is induced by recombinant strains of v. cholerae that produce no subunit of ch ...	1991	2065905
[surveys on the contamination of marine fish with non-o1 vibrio cholerae and vibrio mimicus and food poisoning cases by these organisms].	the present paper describes the relationship between the contamination with non-o1 vibrio cholerae and vibrio mimicus of marine fish, with special reference to the seasonal variation and the concentration of contamination, and the actual cases of domestic food poisoning by these organisms. a 10 year survey revealed that non-o1 vibrio cholerae (non-o1 v. cholerae) strains were frequently isolated from fish during the summer season with some variations from one year to another, and isolates from f ...	1991	2066602
cholera--new york, 1991.	through june 26, 1991, cholera has been reported from seven countries in the western hemisphere: brazil, chile, colombia, ecuador, mexico, peru, and the united states. in the united states, a total of 14 confirmed cases of epidemic-associated cholera have been reported among persons in florida (one) (1), georgia (one) (2), new jersey (eight) (1), and new york (four). this report summarizes information regarding the four cases reported in new york and describes a new laboratory procedure used to ...	1991	2072887
localization of protective epitopes within the pilin subunit of the vibrio cholerae toxin-coregulated pilus.	from a collection of monoclonal antibodies (mabs) that recognize the native structure of the toxin-coregulated pilus of vibrio cholerae, two protective mabs (16.1 and 169.1) were used to localize the corresponding epitopes on the pilus. these mabs were shown to specifically recognize the carboxyl half of the tcpa pilin subunit, as determined by their recognition of proteolytic fragments and hybrid pilin proteins. the positions of the epitopes were precisely determined through the use of overlapp ...	1991	1702758
gmp-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction.	gmp-140 is a rapidly inducible receptor for neutrophils and monocytes expressed on activated platelets and endothelial cells. it is a member of the selectin family of lectin-like cell surface molecules that mediate leukocyte adhesion. we used a radioligand binding assay to characterize the interaction of purified gmp-140 with human neutrophils. unstimulated neutrophils rapidly bound [125i]gmp-140 at 4 degrees c, reaching equilibrium in 10-15 min. binding was ca2+ dependent, reversible, and satur ...	1991	1704009
positive transcriptional regulation of an iron-regulated virulence gene in vibrio cholerae.	we have previously described a virulence gene in vibrio cholerae (irga) that is more than 850-fold regulated in response to iron. negative regulation of irga by iron occurred at the transcriptional level, and there was a dyad symmetric nucleotide sequence in the vicinity of the irga promoter homologous to the fur binding site in escherichia coli. when irga was cloned into e. coli, we showed that transcription of irga required 900 base pairs of dna upstream of the irga promoter that contained an ...	1991	1705025
new model for analysis of mucosal immunity: intestinal secretion of specific monoclonal immunoglobulin a from hybridoma tumors protects against vibrio cholerae infection.	secretory immunoglobulin a (siga) plays a role in defense against vibrio cholerae and other microorganisms that infect mucosal surfaces, but it is not established whether siga alone can prevent disease. we report here a strategy for identifying the antigen specificities of monoclonal siga antibodies that are capable of providing such protection. iga hybridomas were generated from peyer's patch lymphocytes after oral immunization with v. cholerae ogawa 395. a clone was selected that produced dime ...	1991	1705246
cholera: ancient scourge on the rise. who announces global plan for cholera control. (25 april 1991).	vibrio cholerae spreads quickly via contaminated water and food, especially in areas with a poor health and sanitation infrastructure. its enterotoxin induces vomiting and huge amounts of watery diarrhea leading to severe dehydration. 80-90% of cholera victims during an epidemic can use oral rehydration salts. a cholera epidemic is now spreading through latin america threatening 90-120 million people (started in january 1991), particularly those in urban slums and rural/mountainous areas. as ...	1991	12317930
sanitation in the time of cholera.	cholera, identified by violent diarrhea, cramps, vomiting, and dehydration, is spreading through peru into colombia, ecuador, child, and brazil. water contaminated with vibrio cholerae is used for washing food and/or drinking thereby transmitting the disease. paho estimates 6 million people in south america may get cholera within the next 3 years. this cholera epidemic is the result of unsanitary conditions in which the urban poor in south america live. in fact, in lima, peru, 40% of the peo ...	1991	12343751
bacterial adhesion to mucosal surfaces.	bacteria utilize one or more of several mechanisms to adhere to mucosal surfaces. members of the enterobacteriaceae and related bacteria typically elaborate adhesive filamentous appendages from their surfaces termed fimbriae (or pili). vibrio cholerae expresses at least three types of these structures that are likely involved in various stages of colonizing the intestine. so far one of these, termed tcp for toxin coregulated pilus, has been shown to be essential for colonization and virulence in ...	1991	12041763
[isolation and identification of vibrio genus microorganisms in the quibu river].	the quibú river sewages were studied during 9 weeks, in order to isolate and characterize vibrio genus microorganisms. twenty moore's hyssops were placed 2 or 3 times a week on the banks of the river, where each of them was kept in a passive capture stay for 24 hours. in all the hyssops placed, vibrio cholerae non-01 were isolated.	1991	9768187
[on the role of nursing in adjustment of the environment].		1991	259095
teenage sexuality.		1991	259025
[laboratory diagnosis of cholera during enterocolitis epidemic at dimitrovgrad in august and september 1989].	epidemy of enterocolitis at dimitrovgrad started on the 15th of august 1989, and lasted for a whole month. 2018 persons became ill. bacteriological cholera tests were performed on 3054 samples of stools from 2558 patients, and of those who were in contact with the sick populations. stool samples were cultured on the alcal peptonic water, alcal agar, and tcbs agar for vibrio isolation. suspicious colonies identification was made by biochemical and serological tests. difference between cholera bio ...	1991	17974375
psychiatric nursing consultation: a study of the referral process.		1991	207865
effectiveness of low-dose lovastatin in lowering serum cholesterol. experience with 56 patients.	this study reviews the progress of 56 consecutive patients with type iia and iib hyperlipoproteinemia following treatment with lovastatin. lovastatin, a potent inhibitor of 3-hydroxy-3-methylglutaryl coenzyme a reductase, has been shown to have a cholesterol-lowering effect in doses ranging from 10 to 80 mg/d. thus far, however, no large study has been performed to show the effectiveness of low-dose lovastatin (20 mg/d) for more than a 6-week duration. fifty-six patients with known coronary arte ...	1991	2393325
[late results of comprehensive treatment of parodontal diseases in patients with crowding of teeth].		1991	270126
radioimmunoscintigraphy of head and neck cancer using 99mtc-labeled monoclonal antibody e48 f(ab')2.	the diagnostic value of 99mtc-labeled monoclonal antibody e48 f(ab')2 (750 mbq, 1 mg) was evaluated in 10 patients with a histologically proven squamous cell carcinoma of the head and neck and with clinical evidence of cervical lymph node involvement. preoperative findings on lymph node status obtained by radioimmunoscintigraphy (ris), computerized tomography, magnetic resonance imaging, and palpation were defined per side (left and/or right side of the neck) as well as per lymph node level (i t ...	1991	1568225
the determination of inferiority feelings and their contribution to psychiatric morbidity.		1991	270365
drugs in the treatment of peptic ulcers.		1991	245282
[anti-smoking campaign in skaraborg district -- nursing personnel only moderately interested].		1991	258079
parametrial calcification in cervical carcinoma patients treated with radioactive gold.	a unique appearance of soft tissue calcification in the pelvis which may be specific for patients treated with parametrial injection of 198au colloid is described. the calcification appears in the area of maximum radiation dose and can appear within 5 years of treatment. it is significant because it occurred in approximately one-half of the cases radiographically reviewed and may be confused with calcification from other etiologies.	1991	183543
[country council organization does nothing on summer situation!].		1991	258041
question from a director of nursing service.		1991	258007
development and use of an inhalation therapy apparatus for small animal practice.		1991	257985
arthroplasty of the temporomandibular joint with use of a vitallium condyle prosthesis: report of three cases.	we have devised a vitallium intramedullary prosthesis to replace the deformed arthritic condyle in cases that require bilateral condylectomy or in rare long-term failures of a single condylectomy. experience has led to modification of this prosthesis. recently a rectangular stem that prevents rotation of the prosthesis in the condylar stub has been added (fig 11). in bilateral condylectomies, replacement of only one side by the vitallium condylar prosthesis is adequate, because it stabilizes the ...	1991	269935
studies in a phenotypic female with 17-alpha-hydroxylase deficiency.	in a 19-year-old phenotypic female (46, xy) with hypertension and hypokalemia, studies confirmed 17-alpha-hydroxylase deficiency. prior to diagnosis she had been considered to have testicular feminization. increased plasma progesterone and urinary pregnanediol levels were present before treatment. increased secretion rates of deoxycorticosterone and corticosterone and abnormally low production of cortisol and aldosterone were present. following treatment with hydrocortisone, plasma progesterone, ...	1991	182944
the one that nearly got away.		1991	258355
editorial: cancer by county.		1991	51152
cobalt-labeled bleomycin--a new radiopharmaceutical for tumor localization. a comparative clinical evaluation with gallium citrate.	the efficacy of 57co-labeled bleomycin as a tumor-scanning agent was evaluated in 50 patients with malignant tumor. in terms of sensitivity, labeled bleomycin was found to be a superior agent for malignant tumor detection when compared with 67ga-citrate (p less than or equal to 0.005). maximum sensitivity (96%) was found in epidermoid carcinomas. because of rapid blood clearance and rapid excretion by the kidneys, interpretation of 57co-bleomycin scans is facilitated.	1991	51080
theophylline and isoniazid.		1991	2288839
[neurosurgical emergencies].		1991	257713
[inflammatory diseases of the maxillofacial region and the function of the adrenal cortex].		1991	208193
expectations of management.		1991	258133
toxigenicity and toxin genes of vibrio cholerae 01 isolated from an artificial aquatic environment.	a simple experiment was carried out to examlne the effect of varlous physicochemical conditions on toxigenicity and toxin genes of vibrio cholerae 01 lsolated from an artificial aquatic environment. all isolated strains, tested by tissue culture assay, dna-dna hybridization and elisa, were cytotoxic to vero cells, did not lose their toxin genes and continued to produce cholera toxin. these results are consistent with the hypothesis that v. cholerae can survive in the environment without losing p ...	1991	24424942
ana's program of certification in nursing administration.		1992	258256
the neurologic examination in patients with central nervous system trauma.	the neurological assessment of the head injured patient must be integrated into the emergency management and daily care of the patient. neurosurgical nurses are in the best position to perform serial neurologic examinations and assess changes in the patient's clinical picture. if this added responsibility is assumed, neurosurgical nurses must take an active role in improving assessment skills. the improved assessment of the head injured patient will result in early intervention in the patient's ...	1992	258248
prenatal testing for birth defects and nursing practice.	recent advances in the prenatal diagnosis of birth defects including amniocentesis, ultrasound and fetoscopy are discussed. indications, technical considerations and limitations are presented. parental reactions to the stresses encountered during the process of prenatal diagnosis are related. implications are identified for nursing care practice in different clinical settings including prenatal diagnostic units, paediatric services, obstetrical services and in the community.	1992	258240
intensive care of the newborn--3. disorders in the newborn--1.		1992	257463
insider-out: the health worker as researcher.	this article discusses two dimensions related to the insider-outsider dilemma in conducting anthropological research in the health setting. one dimension concerns advantages of the insider's special knowledge in selecting and conducting successful research in a health institution. the other dimension deals with the ambiguity and confusion of roles attributed by staff to the investigator who may be seen as practitioner as well as social scientist. the patient may also see the researcher as perfor ...	1992	257409
[application of controlled electric discharge to the construction of overlay dentures].		1992	1793590
[rehabilitation of psychiatric patients].		1992	257302
erysipelas: facial lymphangitis.	two cases of facial lymphangitis have been described, and the pathophysiology of erysipelas has been discussed. now rarely observed by hospital residents, this potentially serious infection can create considerable diagnostic confusion in medical as well as dental services. rapid response of erysipelas to penicillin therapy is the modern expectation.	1992	265381
changes in nasal profile after maxillary advancement in cleft and non-cleft patients.	based on 25 cases with unilateral clefts of lip, alveolus and palate with retromaxillism (clap) and 25 cases with pure retromaxillism (rm) the effect on the nose of advancing the maxilla by a le fort i osteotomy is analyzed. it can be shown that on average the base of the nose which is at the same time the base of the upper lip, follows the base of the maxilla in the ratio of 4 : 7 while the nasal tip is advanced in a ratio of 2 : 7. this means that to achieve a specified advancement of the nasa ...	1992	265348
current trends in nurse education in the united kingdom.		1992	257142
intelligence quotient in offspring of oral and non-oral contraceptive. second part.		1992	265147
a musical box of therapy.		1992	257566
isolated histiocytosis x of the hypothalamus. case report.	failure to consider histiocytosis x in the differential diagnosis of hypothalamic masses may lead to inappropriate empirical radiotherapy. this report concerns a case of histiocytosis x of the hypothalamus, unassociated with systemic histiocytic lesions, in which early biopsy allowed specific therapy with subsequent total regression of the lesion.	1992	310452
panoramic radiography--an adjunct.		1992	265050
layman's guide to common complaints. 6. earache.		1992	257651
nursing management: past, present and future. the teacher-manager and leader.		1992	257646
nursing management: past, present and future. a better service to patients.		1992	257645
nursing management: past, present and future.		1992	257643
[new diagnostic possibilities in leukemias].		1992	310386
schistosomiasis japonica of liver.		1992	265025
effects of msh/acth 4--10 on memory, attention and endogenous hormone levels in women.		1992	205892
mercury complexes of thionicotinamide adenine dinucleotide.	one-to-one mercury complexes of thionicotinamide adenine dinucleotide (tnad+) were prepared by using hgso4 and hg(ch3 coo-)2. optical absorption spectroscopy indicated that the mercury probably binds to the tnad+ through the thio-keto group on the pyridine ring. x-ray diffraction patterns of crystals of mitochondrial malate dehydrogenase soaked in solution containing tnad+ . mercury complex indicated binding and the x-ray intensity differences are different from mercurials alone.	1992	207356
a two-rooted maxillary central incisor.		1992	265496
medical manpower.		1992	265454
stress due to nuclear accident: a survey of an employee population.		1992	257251