Publications

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effects of x-ray treatments on pathogenic bacteria, inherent microflora, color, and firmness on whole cantaloupe.inactivation of inoculated escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on whole cantaloupes using x-ray at different doses (0.1, 0.5, 1.0, 1.5, and 2.0 kgy) was studied. the effect of x-ray on quality parameters (color and texture) of untreated and treated whole cantaloupes was instrumentally determined. the effect of x-ray on microflora counts (mesophilic counts, psychrotrophic counts and yeast and mold counts) of untreated and treated whole canta ...201222560020
shigella flexneri utilize the spectrin cytoskeleton during invasion and comet tail generation.the spectrin cytoskeleton is emerging as an important host cell target of enteric bacterial pathogens. recent studies have identified a crucial role for spectrin and its associated proteins during key pathogenic processes of listeria monocytogenes and salmonella typhimurium infections. here we investigate the involvement of spectrin cytoskeletal components during the pathogenesis of the invasive pathogen shigella flexneri.201222424399
development and evaluation of a multiplex pcr for simultaneous detection of five foodborne pathogens.to develop a rapid multiplex pcr method for simultaneous detection of five major foodborne pathogens (staphylococcus aureus, listeria monocytogenes, escherichia coli o157:h7, salmonella enteritidis and shigella flexneri, respectively).201222268759
bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells.bacterial pathogens have evolved specific effector proteins that, by interfacing with host kinase signalling pathways, provide a mechanism to evade immune responses during infection. although these effectors contribute to pathogen virulence, we realized that they might also serve as valuable synthetic biology reagents for engineering cellular behaviour. here we exploit two effector proteins, the shigella flexneri ospf protein and yersinia pestis yoph protein, to rewire kinase-mediated responses ...201222820255
complete nucleotide sequence of pvqs1 containing a quinolone resistance determinant from salmonella enterica serovar virchow associated with foreign travel.nalidixic acid-resistant salmonella enterica serovars kentucky (n = 5) and virchow (n = 6) cultured from individuals were investigated for the presence of plasmid-mediated quinolone resistance (pmqr) determinants.201222553140
[antagonistic activity of lactobacilli of the colon].study of antagonistic activity of lactobacilli of the colon against members of its autochthonous bacterial flora and agents of some acute infectious and chronic diseases of the gastrointestinal tract.201222442970
studies on shigella boydii infection in caenorhabditis elegans and bioinformatics analysis of immune regulatory protein interactions.shigella boydii causes bacillary dysentery or shigellosis and generates a significant burden in the developing nations. s. boydii-mediated infection assays were performed at both physiological and molecular levels using caenorhabditis elegans as a host. continuous exposure of worms to s. boydii showed a reduced life span indicating the pathogenicity of shigella. quantitative real-time pcr analysis was performed to analyze the expression and regulation of host specific candidate-antimicrobial gen ...201222841995
outer membrane vesicles of shigella boydii type 4 induce passive immunity in neonatal mice.like most other gram-negative bacteria, shigella releases outer membrane vesicles (omvs) into the surrounding environment during growth. in this study, we have exploited omvs of shigella as a protective immunogen in a mice model against shigellosis. distinctive vesicle secretion was noticed from different shigella strains. among them, shigella boydii type 4 (bch612) was secreting relatively higher amounts. we immunized female adult mice orally with 32 μg of purified shigella boydii type 4 (bch61 ...201222762732
effect of bovine lactoferrin on the minimum inhibitory concentrations of ampicillin and trimethoprim-sulfamethoxazole for clinical shigella spp. strains.here, we determined the effect of bovine lactoferrin (blf) on the minimum inhibitory concentration (mic) of ampicillin and trimethoprim-sulfamethoxazole in shigella . using a microdilution method, the mic was determined in the presence or absence of bovine lactoferrin (10 mg/ml) on 88 shigella strains (56 shigella flexneri , 15 shigella boydii , 13 shigella sonnei , and 4 shigella dysenteriae ) previously isolated from peruvian children <2 years old. a fold change of 2 or more in mic values was ...201222397495
antimicrobial-resistant shigella infections from iran: an overlooked problem?in this study, we wanted to assess the level of antimicrobial resistance, the presence of genes encoding resistance to cephalosporins and plasmid-mediated quinolone resistance (pmqr), and genetic relatedness among shigella isolates obtained from iranian patients.201222345385
quinolone-resistant shigella flexneri isolated in a patient who travelled to india.we report a recent case in which ciprofloxacin-resistant shigella flexneri was isolated from a 23-yr-old female patient with a history of travel to india. prior to her admission to our internal medicine department, she experienced symptoms of high fever and generalized weakness from continuous watery diarrhea that developed midway during the trip. s. flexneri was isolated from the stool culture. despite initial treatment with ciprofloxacin, the stool cultures continued to show s. flexneri growth ...201222950074
absence of o antigen suppresses shigella flexneri icsa autochaperone region mutations.the shigella flexneri icsa (virg) protein is a polarly distributed autotransporter protein. icsa functions as a virulence factor by interacting with the host actin regulatory protein n-wasp, which in turn activates the arp2/3 complex, initiating actin polymerization. formation of f-actin comet tails allows bacterial cell-to-cell spreading. although various accessory proteins such as periplasmic chaperones and the β-barrel assembly machine (bam) complex have been shown to be involved in the expor ...201222936034
identification of a divergent o-acetyltransferase gene oac 1b from shigella flexneri serotype 1b strains.shigella flexneri is a leading cause of bacterial dysentery in developing countries. among the 15 known serotypes, four (1b, 3a, 3b and 4b) contain a group 6 epitope due to an acetyl group connected to the o-2 position of rhamnose iii on the tetrasaccharide structure of the lipopolysaccharide. o-acetyltransferase encoded by a bacteriophage, sf6, mediates the acetylation reaction. we found that the oac gene in serotype 1b strains was very different from that in serotypes 3a, 3b and 4b strains and ...201226038428
shigella flexneri bacteremia in adult. 201222923931
molecular profiling of antimicrobial resistance and integron association of multidrug-resistant clinical isolates of shigella species from faisalabad, pakistan.bacillary dysentery, common in developing countries, is usually caused by shigella species. a major problem in shigellosis is the rapid emergence of multidrug-resistant strains. this is the first detailed molecular study on drug resistance of shigella isolates from the faisalabad region of pakistan. ninety-five shigella isolates obtained after screening of 2500 stool samples were evaluated for in vitro resistance to commonly used antimicrobial agents; the presence or absence of 20 of the most re ...201222906205
bacterial secretion and the role of diffusive and subdiffusive first passage processes.by funneling protein effectors through needle complexes located on the cellular membrane, bacteria are able to infect host cells during type iii secretion events. the spatio-temporal mechanisms through which these events occur are however not fully understood, due in part to the inherent challenges in tracking single molecules moving within an intracellular medium. as a result, theoretical predictions of secretion times are still lacking. here we provide a model that quantifies, depending on the ...201222879888
emergence of a novel shigella flexneri serotype 1d in china.we report on the isolation of 5 shigella flexneri strains displaying a novel serotype, 1d, that shares serologic features from both s. flexneri serotypes 1a and x. the 1d strains contained serotype-converting bacteriophages sfi and sfx in tandem on the chromosome. these strains were likely originated from serotype x strains through sfi infection.201222858548
characterization of lipid a profiles from shigella flexneri variant x lipopolysaccharide.in developing countries, shigella flexneri (sf) is the major causative agent of the endemic shigellosis (bacillary dysentery) responsible annually for one million fatalities mostly among infants. lipopolysaccharides (lpss) are characteristic components of the outer membrane of the overwhelming majority of gram-negative bacteria. since lipid a is essential for the viability of the gram-negative bacteria, it is subject to extensive chemical studies with new analytical techniques.201222847700
virb-mediated positive feedback control of the virulence gene regulatory cascade of shigella flexneri.shigella flexneri is a facultative intracellular pathogen that relies on a type iii secretion system and its associated effector proteins to cause bacillary dysentery in humans. the genes that encode this virulence system are located on a 230-kbp plasmid and are transcribed in response to thermal, osmotic, and ph signals that are characteristic of the human lower gut. the virulence genes are organized within a regulatory cascade, and the nucleoid-associated protein h-ns represses each of the key ...201222821978
[analysis of etiological surveillance results of shigella spp between 2009 and 2010 in henan province].to explore the etiologic characteristics of bacillary dysentery found in henan province, between year 2009 and 2010.201222800632
[multilocus variable-number tandem-repeat analysis for molecular subtypes of shigella isolates in beijing].selecting variable-number tandem-repeat (vntr) loci for different serogroups of shigella spp to explore and establish multilocus variable-number tandem-repeat analysis (mlva) method, in order to study the molecular characteristic of the isolated strains.201222800631
identification of critical residues of the serotype modifying o-acetyltransferase of shigella flexneri.thirteen serotypes of shigella flexneri (s. flexneri) have been recognised, all of which are capable of causing bacillary dysentery or shigellosis. with the emergence of the newer s. flexneri serotypes, the development of an effective vaccine has only become more challenging. one of the factors responsible for the generation of serotype diversity is an lps o-antigen modifying, integral membrane protein known as o-acetyltransferase or oac. oac functions by adding an acetyl group to a specific o-a ...201222793174
molecular dynamics simulations of carbohydrate-mimetic haptens in complex with a complementary anti-carbohydrate antibody.the monoclonal antibody sya/j6 is specific for the o-polysaccharide of the shigella flexneri y bacterium. two haptens, a pentasaccharide and a mimetic octapeptide, bind to sya/j6 with moderate binding affinities. in a previous attempt to obtain improved binding affinity to sya/j6, two glycopeptide chimeras (α-glycopeptide and β-glycopeptide) were designed based on the structures of the pentasaccharide and the octapeptide, as well as a molecular docking study. despite the overall fit of the ligan ...201222789717
shigella flexneri bacteremia in two immune-competent adult travelers.shigella bacteremias are uncommon in immune-competent adults. we report two cases of shigella flexneri bacteremia that occurred in healthy young travelers, who recovered. self-medication with loperamide and ibuprofen without antibiotics (case 1) and concomitant falciparum malaria (case 2) were the only co-morbidities found in our two patients.201222776390
[construction of prokaryotic expression vectors for tandem affinity purification].to construct prokaryotic expression vectors suitable for tandem affinity purification to study protein-protein interactions in bacteria.201222712409
the normal chain length distribution of the o antigen is required for the interaction of shigella flexneri 2a with polarized caco-2 cells.shigella flexneri causes bacillary dysentery in humans. essential to the establishment of the disease is the invasion of the colonic epithelial cells. here we investigated the role of the lipopolysaccharide (lps) o antigen in the ability of s. flexneri to adhere to and invade polarized caco-2 cells. the s. flexneri 2a o antigen has two preferred chain lengths: a short o antigen (s-oag) regulated by the wzzb protein and a very long o antigen (vl-oag) regulated by wzz phs2. mutants with defined de ...201222688980
nlrp10 enhances shigella-induced pro-inflammatory responses.members of the nlr family evolved as intracellular sensors for bacterial and viral infection. however, our knowledge on the implication of most of the human nlr proteins in innate immune responses still remains fragmentary. here we characterized the role of human nlrp10 in bacterial infection. our data revealed that nlrp10 is a cytoplasmic localized protein that positively contributes to innate immune responses induced by the invasive bacterial pathogen shigella flexneri. sirna-mediated knock-do ...201222672233
two live attenuated shigella flexneri 2a strains wrsf2g12 and wrsf2g15: a new combination of gene deletions for 2nd generation live attenuated vaccine candidates.shigella infections are a major cause of inflammatory diarrhea and dysentery worldwide. first-generation virg-based live attenuated shigella strains have been successfully tested in phase i and ii clinical trials and are a leading approach for shigella vaccine development. additional gene deletions in sena, senb and msbb2 have been engineered into second-generation virg-based shigella flexneri 2a strains producing wrsf2g12 and wrsf2g15. both strains harbor a unique combination of gene deletions ...201222658966
roles of h2 uptake hydrogenases in shigella flexneri acid tolerance.hydrogenases play many roles in bacterial physiology, and use of h(2) by the uptake-type enzymes of animal pathogens is of particular interest. hydrogenases have never been studied in the pathogen shigella, so targeted mutant strains were individually generated in the two shigella flexneri h(2)-uptake enzymes (hya and hyb) and in the h(2)-evolving enzyme (hyc) to address their roles. under anaerobic fermentative conditions, a hya mutant strain (hya) was unable to oxidize h(2), while a hyb mutant ...201222628482
[development of a singleplex pcr assay targeting o-antigen modification genes for molecular serotyping of shigella flexneri].to develop a singleplex pcr assay targeting o-antigen modification genes for molecular serotyping of shigella (s.) flexneri.201222613389
antimicrobial susceptibility patterns of shigella isolates in foodborne diseases active surveillance network (foodnet) sites, 2000-2010.treatment of shigellosis with appropriate antimicrobial agents shortens duration of illness and bacterial shedding, but resistance to commonly used agents is increasing.201222572670
genome sequence of shigella flexneri serotype 5a strain m90t sm.bacteria of the genus shigella are a major cause of death worldwide (l. von seidlein et al., plos med. 3:e353, 2006). we sequenced the genome of shigella flexneri strain m90t sm (serotype 5a) and compared it to the published genome sequence of s. flexneri strain 8401 (serotype 5b).201222582379
shigella flexneri effectors ospe1 and ospe2 mediate induced adherence to the colonic epithelium following bile salts exposure.shigella flexneri is a gram-negative pathogen that invades the colonic epithelium. while invasion has been thoroughly investigated, it is unknown how shigella first attaches to the epithelium. previous literature suggests that shigella utilizes adhesins that are induced by environmental signals, including bile salts, encountered in the small intestine prior to invasion. we hypothesized that bile would induce adherence factors to facilitate attachment to colonic epithelial cells. to test our hypo ...201222571618
prevalence of plasmid-mediated quinolone resistance determinants in association with β-lactamases, 16s rrna methylase genes and integrons amongst clinical isolates of shigella flexneri. 201222556328
hierarchies of host factor dynamics at the entry site of shigella flexneri during host cell invasion.shigella flexneri, the causative agent of bacillary dysentery, induces massive cytoskeletal rearrangement, resulting in its entry into nonphagocytic epithelial cells. the bacterium-engulfing membrane ruffles are formed by polymerizing actin, a process activated through injected bacterial effectors that target host small gtpases and tyrosine kinases. once inside the host cell, s. flexneri escapes from the endocytic vacuole within minutes to move intra- and intercellularly. we quantified the fluor ...201222526677
self-association of the shigella flexneri icsa autotransporter protein.the icsa autotransporter protein is a major virulence factor of the human intracellular pathogen shigella flexneri. icsa is distributed at the poles in the outer membrane (om) of s. flexneri and interacts with components of the host actin-polymerization machinery to facilitate intracellular actin-based motility and subsequent cell-to-cell spreading of the bacterium. we sought to characterize the biochemical properties of icsa in the bacterial om. chemical cross-linking data suggested that icsa e ...201222516224
standardization and international multicenter validation of a pulsenet pulsed-field gel electrophoresis protocol for subtyping shigella flexneri isolates.shigella flexneri is one of the agents most frequently linked to diarrheal illness in developing countries and often causes outbreaks in settings with poor hygiene or sanitary conditions. travel is one of the means by which s. flexneri can be imported into developed countries, where this pathogen is not commonly seen. a robust and discriminatory subtyping method is needed for the surveillance of s. flexneri locally and regionally, and to aid in the detection and investigation of outbreaks. the p ...201222506731
epidemiological study of shigellosis in an urban area of argentina.shigellosis represents one of the main causes of bloody diarrhoea in south america. this study aimed to establish the incidence of shigellosis in an urban zone of buenos aires, argentina, by examining the type of shigella and living conditions associated with this infection.201222505441
ongoing outbreak of shigella flexneri serotype 3a in men who have sex with men in england and wales, data from 2009-2011.diagnoses of shigella flexneri in the united kingdom (uk) are usually travel-related. however, since 2009, there has been an overall increase in uk-acquired cases. the health protection agency has been investigating a national outbreak of s. flexneri detected in 2011 and which is still ongoing. cases occurred mostly in men who have sex with men and were of serotype 3a. the investigation aimed at obtaining epidemiological data to inform targeted outbreak management and control.201222490381
comparison of the prevalence and changing resistance to nalidixic acid and ciprofloxacin of shigella between europe-america and asia-africa from 1998 to 2009.shigella is becoming an increasing public health problem due to development of multiple antimicrobial resistance, frequently resulting in treatment failure. a systematic review was conducted based on a literature search of computerised databases. random or fixed-effects models were used, based on the p-value considering the possibility of heterogeneity between studies, for meta-analysis. statistical analyses were performed using stata 10.0. in the area of asia-africa, resistance rates to nalidix ...201222483324
ultrastructural analysis of ipad at the tip of the nascent mxih type iii secretion apparatus of shigella flexneri.shigella flexneri is a gram-negative enteric pathogen that is the predominant cause of bacillary dysentery. shigella uses a type iii secretion system to deliver effector proteins that alter normal target cell functions to promote pathogen invasion. the type iii secretion apparatus (t3sa) consists of a basal body, an extracellular needle, and a tip complex that is responsible for delivering effectors into the host cell cytoplasm. ipad [ipa (invasion plasmid antigen)] is the first protein to local ...201222480614
structural characterization of closely related o-antigen lipopolysaccharide (lps) chain length regulators.the surface o-antigen polymers of gram-negative bacteria exhibit a modal length distribution that depends on dedicated chain length regulator periplasmic proteins (polysaccharide co-polymerases, pcps) anchored in the inner membrane by two transmembrane helices. in an attempt to determine whether structural changes underlie the o-antigen modal length specification, we have determined the crystal structures of several closely related pcps, namely two chimeric pcp-1 family members solved at 1.6 and ...201222437828
characterization of the promoter, mxie box and 5' utr of genes controlled by the activity of the type iii secretion apparatus in shigella flexneri.activation of the type iii secretion apparatus (t3sa) of shigella flexneri, upon contact of the bacteria with host cells, and its deregulation, as in ipab mutants, specifically increases transcription of a set of effector-encoding genes controlled by mxie, an activator of the arac family, and ipgc, the chaperone of the ipab and ipac translocators. thirteen genes carried by the virulence plasmid (ospb, ospc1, ospd2, ospd3, ospe1, ospe2, ospf, ospg, vira, ipah1.4, ipah4.5, ipah7.8 and ipah9.8) and ...201222427898
calpain activation by the shigella flexneri effector vira regulates key steps in the formation and life of the bacterium's epithelial niche.the enteropathogen shigella flexneri invades epithelial cells, leading to inflammation and tissue destruction. we report that shigella infection of epithelial cells induces an early genotoxic stress, but the resulting p53 response and cell death are impaired due to the bacterium's ability to promote p53 degradation, mainly through calpain protease activation. calpain activation is promoted by the shigella virulence effector vira and dependent on calcium flux and the depletion of the endogenous c ...201222423964
identification of the bile salt binding site on ipad from shigella flexneri and the influence of ligand binding on ipad structure.type iii secretion (tts) is an essential virulence factor for shigella flexneri, the causative agent of shigellosis. the shigella tts apparatus (ttsa) is an elegant nanomachine that is composed of a basal body, an external needle to deliver effectors into human cells, and a needle tip complex that controls secretion activation. ipad is at the tip of the nascent ttsa needle where it controls the first step of tts activation. the bile salt deoxycholate (doc) binds to ipad to induce recruitment of ...201222423359
the shigella flexneri effector ospi deamidates ubc13 to dampen the inflammatory response.many bacterial pathogens can enter various host cells and then survive intracellularly, transiently evade humoral immunity, and further disseminate to other cells and tissues. when bacteria enter host cells and replicate intracellularly, the host cells sense the invading bacteria as damage-associated molecular patterns (damps) and pathogen-associated molecular patterns (pamps) by way of various pattern recognition receptors. as a result, the host cells induce alarm signals that activate the inna ...201222407319
structure of a type iii secretion needle at 7-å resolution provides insights into its assembly and signaling mechanisms.type iii secretion systems of gram-negative bacteria form injection devices that deliver effector proteins into eukaryotic cells during infection. they span both bacterial membranes and the extracellular space to connect with the host cell plasma membrane. their extracellular portion is a needle-like, hollow tube that serves as a secretion conduit for effector proteins. the needle of shigella flexneri is approximately 50-nm long and 7-nm thick and is made by the helical assembly of one protein, ...201222388746
enhanced type iii secretion system expression of atypical shigella flexneri ii:(3)4,7(8).we aimed at evaluating the virulence of atypical shigella flexneri ii:(3)4,7(8) by dna microarray and invasion assay.201224159518
analysis of different prognostic indicators for malnutrition and shigella flexneri infection among the children in bangladesh.the present study investigates into some socio-economic, demographic, and nutritional features that can predispose bangladeshi children to malnutrition and shigella flexneri infection. significant prognostic indicators associated with malnutrition were mother's illiteracy (unadjusted odds ratio, or = 2.580; 95% confidence interval, ci = 1.134-5.867 and adjusted odds ratio, ora, 3.814; 95% ci = 1.124-12.943), low birth weight (or = 3.143; 95% ci = 1.2-8.232 and ora = 2.404; 95% ci = 0.870-6.644) ...201223997331
[the men behind the syndrome: johannes peutz and harold jeghers. the explorers of an underdiagnosed condition with intestinal polyps and mucocutaneous pigmentations]. 20123302567
[soviet urology in the period of perestroĭka]. 20123438946
blood venom antigen levels after malayan pit viper bite. 20123445336
smallpox in australia. 20123883104
comparative bio-availability of theophylline whole and halved sustained-release tablets.in a randomised, multiple-dose, cross-over study in 14 healthy volunteers, plasma theophylline concentrations were compared during a 12-hour dosing interval after repeated administration of theophylline (euphyllin retard; byk gulden) as whole and halved tablets. bio-availability of theophylline from the halved tablets relative to the whole tablets was: 116% (100%, 134%) for the extent of absorption as judged by the area under the concentration time curve (auc) and 115% (99%, 135%) for the rate o ...20123299763
color drawings: a means of identifying problems in children.between the ages of seven and nine years, the child develops a schema or way to draw his version of the world. drawings are revealing in that they can help assess a child's behavior. figure drawings are useful in determining general cognitive development, self-image, and level of maturity (harris, 1963; dileo, 1973; dileo, 1983; and koppitz, 1968). as the child progresses through developmental stages in perceiving and using color, she similarly progresses in her drawing abilities. distinction, i ...20123272927
transition to flight nursing: three experiences. 20123302460
elastic properties of relaxed, activated, and rigor muscle fibers measured with microsecond resolution.tension responses due to small and rapid length changes (completed within 40 microseconds) were obtained from skinned single-fiber segments (4- to 7-mm length) of the iliofibularis muscle of the frog incubated in relaxing, rigor, and activating solution. the fibers were skinned by freeze-drying. the first 500 microseconds of the responses for all three conditions could be described with a linear model, in which the fiber is regarded as a rod composed of infinitesimally small identical segments, ...20123266558
arteriolar oxygen reactivity: where is the sensor?the hypothesis that arterioles are intrinsically sensitive to oxygen was tested by comparing arteriolar diameter responses with local and global po2 changes in superfused hamster cheek pouch preparations. local po2 changes were produced by microapplication of fluid onto the surface of occluded or unoccluded aparenchymal arterioles or by cannulation and perfusion of arterioles in situ. global changes refer to po2 changes in the superfusate flowing over the entire preparation. local, effective po2 ...20123318502
[an epidemiologic survey of treated and non-treated occlusion]. 20123509632
a partial solution to the base rate problem of the kappa statistic. 20123358648
the relevance of transcultural nursing to international nursing.does transcultural nursing as taught in united states' nursing education programmes adequately prepare us nurses for experiences in international health as they assist with the implementation of changes in another country's health care delivery system? that's the question posed by lydia desantis, who is concerned that us nurses need a broader perspective of health care and culture in order to effect changes needed at the national level of policy and decisionmaking to meet the challenges of the d ...20123417392
[efficacy of the conservative treatment of patients with inoperable esophageal cancer].the results of gamma-beam therapy of esophageal cancer leave much to be desired. however betatron therapy improves them. to compare the results of esophageal cancer therapy 4 therapeutic modalities were employed: the 1st group--chemoradiotherapy (methotrexate at a weekly dose of 45 mg), the 2nd group--chemoradiotherapy (methotrexate at a dose of 10 mg alternating with prospidin at a dose of 200 mg), the 3rd group--radiotherapy (inhibitory radiation), the 4th group--radiotherapy (gamma-beam thera ...20123339977
1 in 4 irish women suffer bone disease. 20123378870
munchausen's syndrome: a case report. 20123503007
coronary artery disease presenting with exercise induced complete heart block. a case report. 20123454398
notifiable diseases in canada. 20123416266
[delayed eruption, enamel hypoplasia and caries in childhood celiac disease]. 20123480411
leakage of cathepsin from lysosomes during an early stage of fixation may be related to background adsorption of horseradish peroxidase (hrp) and loss of cell surface hrp-binding sites. 20123335776
focal dermal hypoplasia syndrome (goltz syndrome): the first dental case report. 20123466146
okt3 rescue in refractory renal rejection.a community hospital-based transplant program participated in the study of orthoclone okt3 as a treatment for refractory renal rejection. a total of 16 patients receiving varied oral immunosuppressive regimens, and developing steroid-resistant acute rejection, were treated with okt3. thirteen of the 16 patients completed a 10-day course of therapy with reversal of rejection in all 13. rerejection occurred in 1 of the 13 patients; the remaining 12 have normal renal function. fever, chills, and di ...20123306423
measurement of monoclonal immunoglobulin concentrations in hybridoma cultures by competitive inhibition enzyme immunoassay. 20123523131
changes in caloric nystagmus induced by vasodilating drugs.systemic blood flow, blood flow to the peripheral skin, middle ear, and temporal bone, and that to the inner ear of normal volunteers was manipulated selectively through the use of vasodilator drugs. the effect of selective vasodilation was monitored via changes in the temporal course of the slow-phase velocity of nystagmus evoked by bithermal caloric irrigation. drug influenced eye velocities were compared with control values measured before drug administration. eye velocities measured after ni ...20123485753
vitamin a in osteo- and rheumatoid arthritis. 20123408838
[clinical competence and medical responsibility in rare cases]. 20123599673
femoral component loosening in hip arthroplasty. cadaver study of subsidence and hoop strain.to aid in understanding loosening following cemented total hip arthroplasty, we conducted a cadaver study of the proximal femur with implanted cobalt-chromium and titanium femoral components of recent design, loaded through the head of the prosthesis. stem subsidence and strain in the proximal femur were measured. after proximal support of the implant collar was removed, we found that cobalt-chromium implants had a greater tendency to subside than titanium implants. subsidence of a femoral compo ...20123425274
keratophakia and keratomileusis. 20123378864
[difficulties in the diagnosis of epilepsy in children].on the basis of an analysis of 3 cases it was shown that the occurrence of seizure-like symptoms with abnormal eeg and history data suggesting possible damage to the central nervous system are not always sufficient for an unequivocal diagnosis of epilepsy and for beginning of long-term antiepileptic treatment which is not without adverse effects on the developing organism of a child. for establishing of correct diagnosis and avoiding of too hasty introduction of anticonvulsant treatment not only ...20123587512
[difficulties in the diagnosis of epilepsy in children].on the basis of an analysis of 3 cases it was shown that the occurrence of seizure-like symptoms with abnormal eeg and history data suggesting possible damage to the central nervous system are not always sufficient for an unequivocal diagnosis of epilepsy and for beginning of long-term antiepileptic treatment which is not without adverse effects on the developing organism of a child. for establishing of correct diagnosis and avoiding of too hasty introduction of anticonvulsant treatment not only ...20123587512
esophageal deviation: pushed or pulled? 20123497534
biologic fixation of a press-fit titanium hip joint endoprosthesis.the rectangular cross section of a straight-stem prosthesis makes a primary stable, rotation-safe fixation possible that is independent of the individual form of the femur. due to the curvature of the femur, a three-dimensional large-area anchorage is achieved along the entire prosthesis length in the corticalis as well as in the spongiosa. the titanium-aluminum-niobium alloy is extremely biocompatible and enables the fast ongrowth of newly formed osseous tissue to act as secondary stabilization ...20123416526
induction and maintenance of swallowing responses in infants with dysphagia.a treatment package was used to induce and maintain swallowing with three infants who did not swallow food or liquid. prior to treatment, they received all nutrition and hydration via gastrostomy tube feedings. the treatment package consisted of least-to-most intrusive physical prompts, an eliciting stimulus, contingent social reinforcement, and repeated trials to induce and maintain swallowing. the design combined elements of reversal and changing criterion designs for all three infants. the pa ...20123417578
[use of effective methods of treating caries and its complications to prevent odontogenic inflammatory processes in children]. 20123467454
what can dental lab software do? 20123457417
the pianist's hand: anthropometry and biomechanics. 20123359991
suspended scaffolding: proper protection reduces fall injuries. 20123347448
creative retention and recruitment. 20123340363
a.i.d. and aids--too close for comfort. 20123338577
kinetics and dynamics of prednisolone. 20123319533
health care delivery to adolescents and young adults by pediatricians. 20123405691
structure/function relationship in macrolide polyene antibiotics. 20123255745
cell-mediated lympholysis in kaposi's sarcoma after renal allograft. report of two cases. 20123538555
long-term response to carbamazepine: a retrospective study.carbamazepine is now used by many clinicians in the treatment of bipolar disorder (bd) refractory to standard treatments, including lithium and neuroleptics. little information is yet available about the utility and efficacy of this novel treatment during long-term use. we carried out a retrospective study of 50 patients (34 with bd) who had received carbamazepine for the treatment of a psychotic disorder. two-thirds (22) of the bd patients and two of the 16 patients with other diagnoses appeare ...20123372707
design of skin flaps with a new technique using templates.patterned templates were constructed to assist in planning, performing, and teaching skin flap surgery. templates can be used to design geometrically accurate flaps and to consider different flap orientation strategies. they can be used to align flap tension forces in proper relationship with relaxed skin tension lines.20123312330
acute orbital pseudotumor with iatrogenic intraorbital air: a diagnostic dilemma.a 13-year-old black female was referred with unilateral granulomatous uveitis and orbital inflammation of sudden onset. hematologic, serologic, bacteriologic, and ultrasonographic studies along with high resolution ct scans of the orbits were employed to determine the diagnosis and appropriate treatment. subconjunctival corticosteroid injection containing air prior to referral obfuscated the ultimate diagnosis of pseudotumor. the pediatric infectious disease service delayed definitive treatment ...20123454372
[pulmonary eosinophilia in asthmatic patients: after radiography, bronchoalveolar lavage].the technique of broncho-alveolar lavage (bal) can be used without difficulty in asthmatic patients. the authors studied 17 patients with high eosinophil counts (greater than 20 p. 100) and followed them up for 6 to 12 months. an increase in total cell counts in bal was observed which was in direct correlation with the percentage of eosinophils; chest x-ray showed no evidence of pulmonary infiltration in any of the cases; 9 patients were steroid dependent. these observations indicate that radiol ...20123706956
to reduce or to integrate: psychiatry's dilemma.three conceptual approaches to psychiatry may be distinguished: the somatic (biological); the psychological (psychodynamic, psychosocial); and the integrative (holistic, biopsychosocial). the first two emerged in the early nineteenth century, the third - in this century. all three continue to vie for influence today. the author traces their historical precursors and discusses their current state. he argues that psychiatrists need to apply methodological reductionism but adopt an integrative orie ...20123518907
smooth the way for safe emergency transfers. 20123375752
insights on death & dying. depression: bottled-up emotions. 20123386914
information systems for the community health services.this paper is concerned with the design and implementation of an information system which fulfils some of the local needs of fourteen nursing and para-medical professions working in the community in a district health authority, whilst satisfying the statutory requirements of the nhs körner steering group for those professions. the implementation of the computer information system was achieved through the participation of those professionals who would eventually use the system. a prototype system ...20123405016
therapeutic activity of lactate 12% lotion in the treatment of ichthyosis. active versus vehicle and active versus a petrolatum cream.lactate 12% lotion was significantly more effective than both its vehicle and a petrolatum-based cream in the treatment of ichthyosis. the treatment regimen was twice-daily application for 4 weeks with evaluations weekly during the treatment period and for 2 weeks after treatment was stopped. vulgaris, lamellar, sex-linked, netherton's, and epidermolytic hyperkeratotic forms of ichthyosis were significantly improved by treatment with lactate 12% lotion. this new therapeutic modality expands the ...20123543073
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