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the effects of sodium cromoglycate on antigen inhalation challenge in two horses affected with chronic obstructive pulmonary disease (copd).80 mg sodium cromoglycate (scg) was administered by inhalation to two copd-affected animals known to have respiratory hypersensitivity to micropolyspora faeni. scg treatment 20-30 minutes prior to inhalation challenge with m. faeni prevented exacerbation of respiratory disease, usually seen 4-8 hours after challenge. duration of protection against antigen challenge after a single scg treatment was 4-5 days. the duration of protection was not prolonged by reducing the frequency of antigen challen ...197915612272
dispersal of aspergillus fumigatus from sewage sludge compost piles subjected to mechanical agitation in open air.aerosolization of the thermophilous fungal opportunist aspergillus fumigatus from mechanically agitated compost piles was examined at a pilot-scale sewage sludge composting facility and two other selected test sites. aerosols of a. fumigatus downwind from stationary compost piles were insignificant in comparison with those downwind from agitated piles. these aerosols were generated by a front-end loader moving and dropping compost. aerial concentrations of the fungus at distances downwind from t ...198016345563
the preparation and partial characterization of antigenic fractions obtained from the mycelial walls of several aspergillus species.extracts with immunological activity were prepared from aspergillus fumigatus, a. flavus, a. terreus, a. niger and a. nidulans. in each case crude mycelial wall was extracted with an aqueous solution of triton x-100 giving detergent-soluble material. further fractionation was achieved by removing the detergent from this solution; the resultant precipitate was removed by centrifugation, and the aqueous supernatant was used as a source of soluble antigens. the sensitivity of these preparations was ...19806157778
mycelial antigens from two strains of aspergillus fumigatus: an analysis by two-dimensional immunoelectrophoresis. 19806160400
trypsinogen-kinase from aspergillus fumigatus.the activation of bovine trypsinogen by an extracellular acid proteinase from a. fumigatus is described. the enzyme activates trypsinogen optimally at ph 3.5 and 32 degrees c. the effect of substrate and enzyme concentrations on the activation has been studied and the km-value has been determined.19806247187
the activity of ketoconazole in mixed cultures of fungi and human fibroblasts. 19806247651
opportunistic infections of the lung. 19806252645
relationship between the house dust and mite allergen by immunochemical and clinical evidence.this investigation was carried out to determine antigenic potency in extracts of house dust, d. pteronyssinus and d. farinae and aspergillus fumigatus with observation of precipiting antibodies in the serum of immunised rabbits. the titre of precipiting antibodies dependens on the mode of immunisation, preparation and concentration of antigens. our investigations revealed that the house dust antigen comprises antigen of d. pteronyssinus, which can clarify the cross-reaction between the antiserum ...19806447446
degradation of humic acids, extracted from manure and soil by some streptomycetes and fungi.humate-decomposing microorganisms were isolated from alluvial soil, farmyard manure and peat by direct plating and enrichment techniques. peat was found to harbour more of these organisms than soil and manure. out of 21 actinomycetes and 6 fungi 9 and 3 isolates, respectively, were found to utilize manure-humate as a sole source of carbon and to a less extent as a source of both carbon and nitrogen in liquid medium. these efficient cultures were identified as streptomyces spec. (a 11 and a 12). ...19806448526
evolutionary origins of vertebrate hormones: substances similar to mammalian insulins are native to unicellular eukaryotes.tetrahymena pyriformis, neurospora crassa, and aspergillus fumigatus that had been grown in simple defined media were extracted with acid ethanol by a classic method for recovering insulin from pancreas. after filtration of the extracts on sephadex g-50, distinct peaks of insulin immunoreactivity were recovered in the region typical of insulin. the gel-filtered material from the tetrahymena had reactivity in the pork insulin radioimmunoassay about equal to its reactivity in the insulin bioassay ...19806449704
immune complexes and humoral response to pseudomonas aeruginosa in cystic fibrosis.we studied the humoral immune status of 51 patients with cystic fibrosis (cf) as compared to 25 patients with other respiratory diseases (rd). cf patients had higher serum concentrations of igg and iga (p less than 0.001), c5, and ch50 (p less than 0.05), than did rd patients. twenty-three % of cf patients had increased ige concentrations. of 32 cf and 1 rd patients colonized with mucoid pseudomonas aeruginosa (pa), 91% had serum precipitins to pa, whereas no precipients were found in patients n ...19806766286
air-borne fungi in the air of barcelona (spain). iii. the genus aspergillus link.during a survey on the presence of species of the genus aspergillus in the air of the city of barcelona (spain), the following species were identified: aspergillus flavus link, a. niger van tieghem, a. fumigatus fresenius, a. clavatus desmazières, a. terreus thom, a. chevalieri (mang.) thom et church, a. niveus bloch, emend. thom et church, a. ochraceus wilhelm, a. versicolor (vuillemin) tiraboschi, and a. amstelodami (mang.) church et thom.19806770271
[about 35 cases of pleural aspergillosis (author's transl)].considering 36 observations, the authors show the relative infrequency of pleural aspergillosis localizations by comparison with pulmonary aspergillomas. twenty four pleural aspergilloses developed on post-operative residual cavities, particularly after pulmonary exeresis and not only for aspergilloma; 11 forms were apparently primitive after therapeutical pneumothorax; 3 anatomo-clinical forms were found, aspergillomas and pyothoraxes were the most frequent. pleural involvement can be associate ...19806770358
cellulase induction in aspergillus fumigatus m 216. 19806771206
diagnosis of invasive aspergillosis by passive hemagglutination assay of antibody.sheep red cells treated with concanavalin a and sensitized with a partially purified aspergillus antigen were used to detect antibody to aspergillus by passive hemagglutination (pha). sera from eight patients with aspergillomas or allergic aspergillosis had pha titers of greater than or equal to 1:800 and antibody detectable by immunodiffusion (id). of 122 hospitalized cancer patients without invasive aspergillosis, 118 had titers of less than or equal to 1:80, 86 of < 1:10, two of 1:160, and tw ...19806772712
preparation of aspergillus fumigatus antigens and their analysis by two-dimensional immunoelectrophoresis.the water-soluble components of aspergillus fumigatus mycelium were partially separated by fractional precipitation with ammonium sulphate. the total protein and neutral-sugar content were determined for each of the four fractions prepared and their immunological activity was examined by double diffusion. partial chemical characterisation of these isolates by polyacrylamide-gel electrophoresis was linked to their precipitability by allowing the separated components to diffuse from the gel into a ...19806774095
the detection of circulating antibodies against antigens from three strains of aspergillus fumigatus. 19806775222
[inactivation of viruses and bacteria in sewage sludge by gamma radiation (author's transl)].the kinetics of inactivation and the resistance to gamma radiation of microorganisms usually to be found in raw sludge were examined with five viruses, three bacteria and a fungus serving as prototypes in comparative studies. all these infectious agents could reliably be inactivated by gamma rays in raw sewage sludge but they were clearly more resistant to gamma rays compared to irradiation in a liquid suspension. the reduction of the virus content required a much higher radiation dose compared ...19806775456
characterization of antigens from aspergillus fumigatus. v. reactivity in immunodiffusion tests with serums from patients with aspergillosis caused by aspergillus flavus, a. niger, and a fumigatus.antigen-rich mycelial extract (me) and fractions of aspergillus fumigatus were compared with culture filtrates of a. fumigatus. a. flavus, and a. niger for their abilities to detect antibody in serums from patients with confirmed aspergillosis caused by these fungi. in double immunodiffusion tests, homologous antigen preparations were more effective than heterologous preparations in detecting precipitins. mycelial extract of a. fumigatus was more effective than a. fumigatus culture filtrate in ...19806776857
serodiagnosis of aspergillus fumigatus antibody in migratory ducks.migrating duck populations in wisconsin were evaluated during a one-year period for exposure to aspergillus fumigatis by two serodiagnostic methods: immunodiffusion and countercurrent immunoelectrophoresis. peak antibody reactivity to sporulating culture extracts of the fungus was detected in serum samples collected in winter. antibody levels did not differ between male and female or adult and juvenile populations.19806776945
the preparation and partial purification of fractions from mycelial fungi with antigenic activity. 19806777666
a notable aspergillus from a mortal aspergilloma of the lung. new aspects of the epidemiology, serodiagnosis and taxonomy of aspergillus fumigatus.different basic mycological observations were made in relation to a fatal case of aspergilloma of the lung in a 45-year-old male. cultural examination of the sputum and autopsy material from the lung revealed the predominant presence of a white fungus along with a scarce growth of the typical aspergillus fumigatus. the white isolate produced conidial heads almost similar to that of a. fumigatus but with apparent septation in the phialides. with the help of the immunodiffusion test and use of the ...19806779440
plasma progesterone concentration in the bovine before abortion or parturition in pregnant animals exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus.pregnant cows in the 4th and 5th month of the gestation were exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus. plasma progesterone concentrations were determined at intervals from before the cows were exposed until they had aborted or calved. the plasma progesterone concentration of the exposed pregnant cattle remained at 3.95 +/- 2.0 ng/ml until 24 to 48 hours before abortion or parturition, when it decreased to below 1.00 ng/ml. this pattern was similar for cattle ex ...19806782916
carotid-cavernous sinus thrombosis caused by aspergillus fumigatus. case report.a case is presented of aspergillus fumigatus granuloma involving the sphenoid sinus, sella turcica, cavernous sinus, and the internal carotid artery. the diagnosis was established by a transsphenoidal biopsy. the infection proved difficult to treat and finally remitted after chemotherapy with a combination of amphotericin b, rifampin (rifampicin), and flucytosine (5-fluorocytosine). the spectrum of aspergillosis of the central nervous system is reviewed, and difficulties in treating this infecti ...19806985651
cutaneous aspergillosis with fatal dissemination in a renal transplant recipient. 19806988066
chemistry of tremorogenic metabolites. i. fumitremorgin a from aspergillus fumigatus. 19806988091
an epidemic of extrinsic allergic alveolitis caused by tap water. 19806988107
prevalence of precipitins in groups at risk of developing hypersensitivity pneumonitis.a study was made of the prevalence of serum precipitins to micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, and pigeon serum in population groups suspected to be at high risk for the development of hypersensitivity pneumonitis. pigeon breeders' sera contained precipitins mainly to pigeon serum (38%) and a. fumigatus (18%). occupants of homes in which forced air heating systems were investigated for the presence of microorganisms reacted mostly with m. faeni (13%) and a. f ...19806988108
[aspergillus fumigatus infection of the lung in cystic fibrosis (author's transl)].in an 8-year-old girl with cystic fibrosis aspergillus fumigatus infection of the lung was diagnosed. pot-plant soil as reservior of aspergillus fumigatus was shown to be the source of inhalable aspergillus fumigatus units within the clinic. cystic fibrosis mucus of the patient acted as nutritive substrate for aspergillus fumigatus. consequent exclusion of pot plants in housing and hospital rooms is considered necessary for prevention of aspergillus fumigatus infection in patients with cystic fi ...19806988190
[taxonomic and ecological and physiological characteristics of aspergillus fumigatus]. 19806988681
fungal infection of the brain: an increasing threat.a 79-year-old, diabetic man with an ear infection had a temporal lobe abscess from which aspergillus fumigatus was cultured. he died despite treatment with amphotericin and rifampin, removal of the abscesss, and left mastoidectomy. because of the ever-increasing number of elderly debilitated patients, the frequency of mild diabetes in the aged, the prevalent use of steroids, antibiotics, cytotoxics and immunosuppressives, and the ubiquity of the organism, aspergillus infections of the brain have ...19806988973
aspergillus nidulans and aspergillus fumigatus as causal agents of bovine mastitis.five cases of bovine udder aspergillosis diagnosed clinically and confirmed by repeated demonstration of fungal hyphae in koh preparations and isolation of the organism from milk sediments are described. in one case the fungus was identified as aspergillus nidulans, in the other 4 cases as a. fumigatus. the most pronounced features in altered udders were multiple abscesses with distinctive colonies of fungal hyphae in the centres, embedded in cell detritus, neutrophils and macrophages. these pur ...19806988991
the preparation of protoplasts from aspergillus fumigatus mycelium.protoplasts have been prepared from the mycelium of aspergillus funigatus, using a lytic enzyme mixture from trichoderma harzianum. a variety of experimental conditions were investigated in order to achieve optimal conditions for protoplast production. electron micrographs showed the preparations to be free of cell-wall. material obtained by this procedure can be used as a source of cytoplasmic antigens for further analysis.19806988993
[ocular mycoses as a complication of hormonal therapy]. 19806989089
serologic immunodiagnosis of of invasive aspergillosis.two sensitive methods, counterimmunoelectrophoresis (cie) and enzyme-linked immunosorbent assay (elisa), for detecting antibodies to aspergilus were used to study serial specimens from patients with histologically proven invasive aspergillosis for measurement of conversion from negative to positive immunoprecipitin reactions and changes in elisa titers during immunosuppression. sera from 12 granulocytopenic patients without invasive aspergillosis served as controls. positive cie reactions were d ...19806989934
sero-diagnosis of invasive aspergillosis: attempts to determine antigen and antibody relevance to infection.attempt was made to define antigens and antisera which might prove useful in diagnosis of invasive aspergillosis in man. a convalescent antiserum (serum from rabbits after liver infection with aspergillus fumigatus conidia) which might be more representative of immunological reaction to fungal growth in vivo, did not react in enzyme-linked immunosorbent assay with commerical antigens which are used at present in attempts to detect antibody response in systemic infections in man. however, this co ...19806990268
[pulmonary mycetomas of the aspergilloma type]. 19806990577
examination of the relationship between pathological changes, immunological response and serum protein concentrations in pregnant sheep inoculated with aspergillus fumigatus.pregnant sheep inoculated with aspergillus fumigatus conidia developed precipitating and latex agglutinating antibodies to mycelial antigens. the titres of these tended to be higher in those animals developing placental or fetal infection than in those which did not. the concentrations of total serum proteins and of albumin, alpha, beta and gamma globulins did not show any consistent changes which could be related to abortion or placental infection. lymphocyte transformation tests on whole blood ...19806991951
necrotising aspergillosis complicating fulminating viral pneumonia. 19806992331
aortotomy site infections: case presentation and review of the literature.a patient with aspergillus fumigatus infection involving an aortotomy site with multiple peripheral emboli following implantation of an aortic valve prosthesis for rheumatic heart disease is described. eleven aortotomy site infections are reviewed from the literature. eight cases were caused by fungi with aspergillus sp accounting for four cases. presenting symptoms were nondiagnostic and multiple. however, the diagnosis of aortotomy site infection should be entertained in patients who have had ...19806992578
antibodies to aspergillus fumigatus in farmers' lung patients measured by enzyme-linked immunosorbent assay (elisa).enzyme-linked immunosorbent assay (elisa) was used to measure antibodies against aspergillus fumigatus in farmers' lung patients, various other pulmonary diseases and healthy controls. both the prevalence and the mean titre of antibodies were significantly higher in farmers' lung patients than in the other groups. there was no difference between controls and patients with bronchial asthma or non-allergic pulmonary diseases. on the other hand, in the group of patients with respiratory diseases of ...19806993041
environmental factors in allergic bronchopulmonary aspergillosis.many workers have suggested that exposure to an environment rich in aspergillus spores is aetiologically significant in allergic bronchopulmonary aspergillosis. this hypothesis has been investigated by means of an environmental survey in which eight urban dwelling patients with this disease and five atopic control patients took part. enquiries were made about contact with known sources of aspergillus fumigatus and air spora studies were conducted in the home. findings were similar for both group ...19806993043
antibodies against aspergillus fumigatus. i. standardization of the antigenic composition.three different stages were found during growth of aspergillus fumigatus as characterized by the changes in ph of the medium: (1) an acidic phase (phase i); (2) a second phase (phase ii) during which the ph rises till values around ph = 8.5; (3) a third phase (phase iii) during which the ph remains constant or drops slightly. during phase i a fast appearance of certain antigenic components was found that is ascribed to an active process of excretion. additional antigenic components appeared in t ...19806993368
antibodies against aspergillus fumigatus ii. identification and quantification by means of crossed immunoelectrophoresis.individual antigenic components of aspergillus fumigatus were identified by crossed immunoelectrophoresis (cie) using pooled rabbit hyperimmune antiserum. patients' sera with enhanced precipitins against a. fumigatus showed antibodies against identical antigenic components in cie. identification of the antigenic components by the intermediate gel technique showed high concentrations of antibodies against a small group of antigenic components. no differences were found in the individual antigenic ...19806993369
hydrolysis of cellulosic wastes by aspergillus fumigatus cellulase. 19806995286
degradation of melanin by aspergillus fumigatus.a strain of aspergillus fumigatus from composted coffee and garden wastes utilized natural deproteinized insect, banana, hair, octopus, and synthetic tyrosine and dopa melanins as sole sources of carbon. with a sucrose supplement, degradation was essentially complete after 50 days in czapek medium ph 6.5 at 30 degrees c. the catabolic rate differed for each substrate pigment, as did the molecular weight distribution of products accumulating in the medium. after incubation with l-[u-14c]melanin, ...19806996615
detection of candida antigenemia in human invasive candidiasis by a new solid phase radioimmunoassay.there is a continuing need to develop reliable non-invasive methods to aid in the early diagnosis of disseminatyed candidiasis. we report the development of a solid phase "sandwich" radioimmunoassay (ria) for the detection of soluble cytoplasmic protein antigens (spa) of candida albicans in patients with systemic candidiasis. spa were prepared by ultrasonic disruption of blastospores of c. albicans. anti-spa igg was covalently linked to a solid phase of polyacrylamide-like microspheres. standard ...19806997211
chromatographic purification and homogeneity of extracellular acid proteinase of aspergillus fumigatus.the extracellular acid proteinase from a. fumigatus was purified by employing several chromatographic procedures and a 164 fold purification with 22% yield was achieved. the homogeneity of the enzyme was confirmed by sds-polyacrylamide gel electrophoresis, immodiffusion and immunoelectrophoresis techniques.19806997232
[usefulness of counterimmunoelectrophoresis for serological diagnosis of pulmonary aspergillosis]. 19806997834
characterization of subspecies from a fungal fatty acid synthetase.fatty acid synthetase from the filamentous fungus, aspergillus fumigatus dissociates during gel filtration on sepharose 6b into two differently sized subspecies with mol. wt. approx. 1.5 x 10(6) and 8 x 10(5). after elution, they readily reform intact molecules, as determined by their enzymic activity (overall synthetase and 3-oxoreductase activities were measured), sedimentation coefficient and appearance in the electron microscope. synthetase was cross-linked with dimethyl suberimidate and the ...19806998497
changes in chemical composition of conidia of aspergillus fumigatus during maturation and germination. 19806999300
effect of five pesticides on the mycelial growth of some soil and pathogenic fungi.the effect of three doses (the lowest is the recommended dose in the field) of 5 pesticides namely: ceresan, orthocid (fungicides), vcs-438 (herbicide), and dursban and dipterex (insecticides) on the mycelial dry weight of aspergillus fumigatus. fusarium moniforme, pencillium italicum, and sclerotium ceprivorum was tested 2, 4, 6, 8 and 10 days after treatment. all the pesticides induced inhibition of mycelial growth of the test fungi. only vcs-438 at the low dose was stimulatory to f. monilifor ...19806999754
[physiological studies on thermophilic hydrocarbon-utilizing hyphae fungi (author's transl)].out of different substrates, thermophilic n-alkane-assimilating hyphae fungi were isolated and the strains of aspergillus fumigatus and mucor lusitanicus selected for physiological investigations. the growth curves, obtained in hydrocarbon and glucose at different temperatures, showed three phases: 1) initial growth phase, 2) linear growth phase, and 3) lysis phase. maximum dry matter and maximum protein content occurred within a range of 35-40 degrees c. during the entire time of growth the dna ...19806999773
prevalence of aspergillus fumigatus in free-living goshawks (accipiter gentilis atricapillus).during the fall migration of 1972 and 1973 unusually large numbers of goshawks (accipiter gentilis atricapillus) were counted at hawk ridge in duluth, minnesota. these birds were sampled for prevalence of fungi of the genus aspergillus. fungi of this genus were recovered from 26 of 49 birds (53%) in 1972 and 4 of 45 (7%) birds in 1973. aspergillosis was confirmed at necropsy in three wild goshawks in 1972, but none in 1973. the disease was further confirmed at necropsy in 8 of 12 (67%) goshawks ...19807001046
[liver abscess in chronic granulomatosis of childhood (author's transl)].a thirteen-year-old boy with an abscess of the liver and recurrent infections since infancy is described. aspergillus fumigatus could be isolated from this surgical treated abscess. polymorphonuclear leukocytes (pmn) and monocytes from the patient show a normal phagocytosis, whereas the oxygen metabolism and the intracellular killing of kastalase-positive microorganismus (staphylococcus aureus, candida albicans) are strikingly decreased. in contrast, the catalase-negative pneumococcus pneumoniae ...19807001145
[case of an allergic form of pulmonary aspergillosis]. 19807001390
extrinsic allergic alveolitis in a sawmill worker. a case report.extrinsic allergic alveolitis, with acute attacks of the disease, developed in a sawmill worker. the diagnosis was based on the history of exposure to moldy wood, on the symptoms and clinical signs, and on physiological and radiographic findings. a microbiological analysis of the ambient air of the patient's work environment showed that the air contained a considerable number of spores, both of fungi and actinomycetes. the most common fungal genus was aspergillus. thermoactinomyces vulgaris pred ...19807001618
allergic bronchopulmonary aspergillosis complicating cystic fibrosis in childhood.allergic bronchopulmonary aspergillosis, known to be associated with cystic fibrosis in older patients, occurred in 7 young atopic children with cystic fibrosis. the diagnosis was suggested by the onset of, or the increase in, asthmatic symptoms accompanied by major chest x-ray changes ranging from total collapse of a lung or lobe to extensive but changing areas of consolidation. each of the children had a blood eosinophilia, positive type i skin tests to aspergillus fumigatus, and reversible ai ...19807002053
prick testing for aspergillus. 19807002364
[sphenoidal aspergilloma]. 19807008316
the use of enzyme-linked immunosorbent assay (elisa) in the diagnosis of farmer's lung.enzyme-linked immunosorbent assay (elisa) was adapted to measure antibodies against aspergillus fumigatus, thermoactinomyces vulgaris and micropolyspora faeni. mycelial and culture fluid antigens were used separately to analyze antibodies in three study groups: farmer's lung patients, patients with bronchitis and healthy control persons. the mycelial antigen of a. fumigatus gave more positive results than the corresponding culture fluid antigen. the situation was reversed for the actinomycetes. ...19807008641
iga antibody against aspergillus fumigatus antigen in patients with allergic bronchopulmonary aspergillosis.iga antibodies against aspergillus fumigatus (af) antigen were measured by a radioimmunoassay in sera from patients with allergic bronchopulmonary aspergillosis (abpa) and compared with iga antibody levels in asthmatics who had positive prick tests to af and normal controls. the assay for iga antibody discriminated between both control groups and the abpa group at two serum dilutions (10(-2) and 10(-3)) but did not discriminate all individual abpa sera from the peak levels of antibody in the con ...19807008988
[primary, disseminated aspergillosis with signs of hilar lymphoadenopathy in chest x-ray (author's transl)]. 19807009943
the effect of calcium on protoplast release from species of aspergillus.the effect of various osmotica and lytic enzyme treatments, on the release of stable protoplasts from aspergillus niger and two strains of aspergillus fumigatus, has been investigated. most osmotica supported protoplast release at a concentration of 0.6 m except for cacl2 and ca(no3)2. supplementation of 0.6 m kcl with 0.2 m cacl2 enhanced release from both species, but depended upon strain of organism, concentration of osmoticum and enzyme system employed. more protoplasts were released at low ...19807010082
the role of aspergillus fumigatus antigens in blood coagulation and platelet function.the effects of aspergillus fumigatus antigens on platelet function of rabbits was studied. a. fumigatus antigens produced spontaneous and irreversible aggregation of platelets. aspirin, indomethacin or lidocaine did not block the antigen-induced aggregation. however, no aggregations occurred when complement depleted plasma was used. no differences were noted between the strains used. our findings show that soluble aspergillus antigens can cause platelet aggregation in vitro, possibly via a compl ...19807010083
case report: antemortem diagnosis of disseminated aspergillosis by nasal scraping. 19807012447
[chemistry, action and pathogenic significance of fungal toxins]. 19807019485
atopic allergy, serum ige and rast specific ige antibodies in patients with cystic fibrosis.the occurrence of allergic diseases is studied in a group of 56 swiss children suffering from cystic fibrosis (cf). 27% of cf children showed allergic manifestations, and 61% of cf children had positive family history for atopic diseases. the occurrence of allergies is increased in homozygotes as well as in heterozygotes for cf. total serum ige values are clearly increased in atopic cf children, but also slightly elevated in cf patients without allergic manifestations. occurrence of specific ige ...19806967864
standardization of allergen extracts by inhibition of rast, skin test, and chemical composition.five allergen extracts of dermatophagoides pteronyssinus, lolium perenne, alternaria tenuis, aspergillus fumigatus and cladosporium herbarum, obtained from four different manufacturers, were examined by inhibition of rast, content of protein and carbohydrate, contents of phosphorylcholine (pc) and tridacnin reactive components, and by skin test. inhibition of rast was used as a primary method for establishing allergenic potency and demonstrated wide variations for each preparation supplied by th ...19807004662
[multifocal fungal infections in patients coming to the laryngologists (author's transl)]. 19807005825
[clinical analysis of the causes of pulmonary hemorrhage]. 19807027207
disseminated aspergillosis treated with amphotericin b and surgery in a boy with chronic granulomatous disease.a case of systemic infection caused by aspergillus fumigatus in a seven-year-old boy suffering from chronic granulomatous disease is described. the fungus had infiltrated his lungs, his left foot and the popliteal and inguinal lymph nodes. amphotericin b, 1 mg/kg daily, was given for three months via a central venous catheter, progressive anaemia made amputation of his left leg necessary. the bone tissue was heavily infiltrated with fungal elements. the regional lymph nodes were also resected be ...19807028632
antigenemia detected by radioimmunoassay in systemic aspergillosis.because of difficulties in antemortem diagnosis of systemic aspergillosis, a radioimmunoassay to an aspergillus fumigatus carbohydrate was developed and evaluated in patients with mycotic or bacterial infections. antigenemia was detected in sera obtained antemortem from four of seven patients with systemic aspergillosis and in pleural fluid from an aspergillus empyema but not in control sera or pleural fluid from 43 patients or 27 normal donors. when characterized with reference to onset of dise ...19807387020
hla-a, b and c and hla-dr antigens in intrinsic and allergic asthma.some 103 patients with asthma and 100 healthy volunteers have been typed for hla-a, b and c and hla-dr antigens. the 103 patients consisted of thirty-three with intrinsic asthma, thirty-four with extrinsic asthma, and thirty-six known to have precipitins to aspergillus fumigatus. no increase in frequency of any of the a, b, c, or dr antigens was found to be significant after correction for the number of comparisons was made. however certain trends comparable to findings in other immunopathic dis ...19807389070
fatal pulmonary psittacosis and aspergillosis. case report of dual infection.a 59-year-old man in previously good health had acute, progressive bronchopneumonia and died after a three-week course of illness, despite treatment with various antibiotics. serologic, microbiologic, and histologic findings indicated infection with both chlamydia psittaci and aspergillus fumigatus. disseminated, invasive aspergillosis sometimes is a complication of chronic respiratory disease, malignancy, or other deficiency of host defenses, but to our knowledge, this is the first report of ps ...19807396594
invasive pulmonary aspergillosis in an apparently nonimmunocompromised host.invasive aspergillosis generally occurs in patients with hematologic malignancies, neutropenia or other severe derangements of host defense. an adult patient without such a predisposition, and without a previous history of susceptibility to infections, had rapidly progressive respiratory failure associated with repeated growth of aspergillus fumigatus on culture of sputum and bronchoscopy specimens. at autopsy he proved to have invasive pulmonary aspergillosis.19807424951
cerebral aspergilloma in a patient with allergic bronchopulmonary aspergillosis.the case is described of a woman of 21 who had suffered since childhood from asthma and allergic bronchopulmonary aspergillosis. during her recovery from a period of severe status asthmaticus she developed widespread patchy lung shadowing, signs of an intracerebral space-occupying lesion and high titres of serum precipitins to aspergillus fumigatus. post-mortem examination revealed aspergillus bronchopneumonia and an intracerebral aspergilloma.19807426370
solubilization of insoluble phosphates by thermophilic fungi.the solubilization of tricalcium phosphate and rock phosphate and assimilation of solubilized p by thermophilic fungi isolated from compost were studied. the solubilization of tricalcium phosphate was greater than that of rock phosphate on inoculation with fungi in liquid medium, but growth of most of the fungi was greater in rock phosphate. torula thermophila solubilized tricalcium phosphate maximally. there was solubilization of rock phosphate in semi-solid lignocellulose medium by aspergillus ...19807469257
[allergic alveolitis following inhalation of mould spores from pot plant earth (author's transl)].acute exogenous allergic alveolitis with the typical symptoms of unproductive cough, dyspnoea on exertion, fever, loss of weight, headache and limb pains was observed in a 24-year-old bank employee. leucocytosis was accompanied by raised esr; pronounced hypoxaemia and marked restrictive ventilatory defects were found. x-ray changes consisted of severely increased fine reticular interstitial shadowing. the differential diagnosis of allergic alveolitis was confirmed by the demonstration of precipi ...19817472183
[a case of aspergillosis involving the frontal sinus and orbit (author's transl)]. 19817043372
microbial aerosols and actinomycetes in etiological considerations of mushroom workers' lungs.spent steamed compost, phase ii compost, and dust emanating from spent compost during dumping of stationary-bed mushroom houses were examined bacteriologically. the total count for spent compost was 16 x 10(8) microorganisms per g. the total count for dust was 333 microorganisms per liter of air. actinomycetes belonging to the genus streptomyces often constituted 90% or more of isolates from dust, whereas mold spores constituted approximately 5%. dust weight averaged 3.4 mg/liter of air and cont ...19817195685
the microbiology of spent mushroom compost and its dust.microorganisms in spent steamed mushroom compost and its dust were enumerated, and identified. some phase ii (indoor composting) compost samples were also examined. steaming of spent compost resulted in a 70-76% reduction in microbial numbers. total counts made with compost fusion agar were approximately two logs greater than those for nutrient agar. the most common bacterial isolate was bacillus licheniformis. the most common actinomycete isolates were streptomyces diastaticus and thermoactinom ...19817197578
the prevalence of immediate positive skin tests in nepalese children.two hundred and ninety-three normal schoolchildren between 5 and 15 years old living in the hills of eastern nepal were tested by the skin prick method for sensitivity to six allergens: dermatophagoides pteronyssinus, aspergillus fumigatus. cladosporium herbarum, penicillium notatum, mixed pollens and mixed threshings. these children were also questioned and examined for symptoms and signs relating to allergic disease. of the children, 20% were skin-prick positive to at least one of the allergen ...19817249341
allergic bronchopulmonary aspergillosis in corticosteroid-dependent asthmatics.forty-two corticosteroid-dependent asthmatics were studied to determine whether their underlying disease might be allergic bronchopulmonary aspergillosis (abpa). the clinical and laboratory characteristics studied included age, sex, atopic status, mean corticosteroid doses, skin tests to aspergillus fumigatus (af), precipitins to af, total serum ige, and chest roentgenograms. twelve patients had four or more of the seven primary criteria used and were considered as the abpa suspect group. furthe ...19817252001
125i-clq-binding and specific antibodies as indicators of pulmonary disease activity in cystic fibrosis.we studied the incidence and levels of circulating immune complexes by the 125i-clq-binding assay in patients with cystic fibrosis in relation to clinical respiratory status and specific igg and ige antibodies to pseudomonas aeruginosa. staphylococcus aureus, aspergillus fumigatus, and candida albicans. overall prevalence of cic was 43%, but 86% of serially studied patients had evidence of cic at some time. patients with acute respiratory exacerbations and deteriorating pulmonary function had a ...19817252678
porcine abortions associated with fungi, actinomycetes, and rhodococcus sp.history, lesions, and results of microbiologic examinations are given for four porcine abortions associated with fungi and one each associated with actinomadura madurae, an aerobic actinomycete, and rhodococcus sp. the fungi were gliomastix sp., petriellidium boydii, aspergillus fumigatus, and exophiala jeanselmei. parvovirus, isolated from one fetus, was the only other infectious agent identified. the presence of fungi, actinomadura madurae, and rhodococcus sp. in fetal and placental lesions in ...19817281459
the effect of 5-fluorocytosine on the synthesis of 80s ribosomes by pathogenic fungi.we studied the influence of 5-fluorocytosine(5fc) on the monomeric 80s ribosomes isolated by gradient ultracentrifugation from candida albicans, cryptococcus neoformans, aspergillus fumigatus and wangiella dermatitidis. labelling of the cultures with 32po4 and [3h]-leucine showed marked inhibition of both ribosomal rna and ribosomal protein synthesized during the contact of the fungi with the drug. the degree of inhibition was very similar for the synthesis of both ribosomal constituents, (mostl ...19817292207
synthesis and in vitro antimicrobial property of o-carborane derivatives.various o-carboranes and nido-type dicarbollide anions have been synthesized and tested for antimicrobial activity. nearly all of the dicarbollide monoanions investigated were active in vitro against fungi such as candida albicans, aspergillus fumigatus, and tricophyton asteroides, as well as against gram-positive bacteria. from a consideration of the structure-activity relationships, it seems most reasonable to conclude that the introduction of lipophilic alkyl or o-carboranyl groups to the hyd ...19817310826
the effect of bacterial and fungal phospholipids on the activity of macrophages.the effect of some bacterial and fungal phospholipids on the functions of macrophage in mice was studied. it has been found that in animals injected with phospholipids from staphylococcus aureus, streptococcus faecalis, bacillus subtilis and aspergillus fumigatus the population of macrophages with increased phagocytic and microbicidal activity appeared. the phospholipids from cryptococcus neoformans and penicillium notatum did not affect the activity of macrophages. administration of the candida ...19817347586
experimental aspergillosis in rabbits.controls, as well as cortisone-treated rabbits, were challenged with aspergillus fumigatus spores intratracheally. animals were sacrificed 3, 7, 14 and 28 days after inoculation and their organs cultured and studied histologically. lesions were produced in both groups, although the cortisone-treated group showed greater pathology and the lesions persisted for a longer period of time than the control groups. histologically, the pulmonary lesions showed granulomatous pulmonary aspergillosis and su ...19817348209
leg paralysis in pheasant poults (phasianus colchicus) due to spinal aspergillosis. 19817041410
antigens of aspergillus fumigatus. 1. purification of a cytoplasmic antigen reactive with sera of patients with aspergillus-related disease.an antigen has been purified from the mycelial cell sap of aspergillus fumigatus (strain 507). the same component appears to be present in the extracellular phase (culture filtrate) in a partially degraded form. the cell sap protein has a structure composed of four polypeptides of 45,000 daltons linked through disulphide bonds. the isoelectric point (5.2-5.6) and carbohydrate content (12.5% neutral hexose) indicate that this protein is an acidic glycoprotein. it shows reactions with 75% of sera ...19817039876
terelisa: the elisa test performed in terasaki plates.the use of terasaki (10 microliter samples) and microtitration (100 microliter samples) plates as the solid phase in enzyme immunoassays was compared. various antigens were used for coating the plates and antibodies present in human sera were evaluated using the same anti-human ig antibody labelled with either beta -galactosidase, alkaline phosphatase, peroxidase or glucose oxidase. the results obtained, either by scoring with the naked eye or by absorbance reading with appropriate densitometers ...19817031134
[negative precipitin test after surgical treatment of pulmonary and pleural aspergillosis]. 19817031619
progressive lung disease in a malt-worker.we described a malt-worker whose initial symptoms suggested extrinsic allergic alveolitis. the ensuing cavitating lung disease, aspergillus fumigatus infection with mycetomata, vigorous immune response, and granulomatous liver disease are unusual features.19817031969
fungal flora in the superficial infections of the skin and nails at lucknow. 19817033130
aspergillus keratitis with intraocular invasion.a healthy 48-year-old man developed aspergillus keratitis following mild corneal trauma. intensive medical therapy, initially empirical, then guided by in vitro sensitivity testing, as well as attempts at surgical excision of the infection, were ultimately unsuccessful. the poor therapeutic response may have been due to fungal penetration of the deep corneal stromal before treatment was initiated. the clinical and histologic features of a keratitis are described and related to fungal keratitis i ...19817033854
[otomycosis in the south of chile]. 19817034110
nasal aspergillosis in three horses.three horses were referred for investigation of a unilateral foul smelling scanty nasal discharge, complicated in one case by intermittent epistaxis. thick purulent material or a mycotic plaque was identified by an endoscopic examination of the middle meatus but in two horses this had to be repeated under general anaesthesia before the abnormalities were detected. aspergillus fumigatus was cultured from all three cases and septate hyphae were identified on smears from lesions. histological exami ...19817034361
[the growth of thermophilic fungi strains aspergillus fumigatus and mucor lusitanicus in n-alkane medium (author's transl)].growth parameters of the thermophilic hydrocarbon utilizing strains aspergillus fumigatus and mucor lusitanicus in n-alkanes (kw)- and glucose (gic)-medium in standing and fermenter cultures were investigated. the temperature limits for growth were dependent on the medium composition. the temperature range for sporulation was smaller than for the hyphal growth. both strains were cultivated on carbon limitation with subsequent determinations of n-alkanes glucose, ammoniumsulfate-nitrogen, phospha ...19817034398
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