Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
effectiveness of beauveria bassiana sensu lato strains for biological control against rhipicephalus (boophilus) microplus (acari: ixodidae) in china.owing to the need to combat the spread of acaricide-resistant ticks, the development of long-term biological control has become a hot topic for tick control. in this study, we investigated the pathogenicity of three beauveria bassiana isolates on the engorged female rhipicephalus (boophilus) microplus ticks using different conidial concentrations. the results showed that b. bassiana b.bat17 was highly pathogenic against engorged r. (b.) microplus females, resulting in lethal time (lt50 and lt90) ...201323652160
transformation of beauveria bassiana to produce egfp in tenebrio molitor for use as animal feed additives.efforts are underway to develop more effective and safer animal feed additives. entomopathogenic fungi can be considered practical expression platforms of functional genes within insects which have been used as animal feed additives. in this work, as a model, the enhanced green fluorescent protein (egfp) gene was expressed in yellow mealworms, tenebrio molitor by highly infective beauveria bassiana erl1170. among seven test isolates, erl1170 treatment showed 57.1% and 98.3% mortality of mealworm ...201323651432
bumblebee venom serine protease increases fungal insecticidal virulence by inducing insect melanization.insect-killing (entomopathogenic) fungi have high potential for controlling agriculturally harmful pests. however, their pathogenicity is slow, and this is one reason for their poor acceptance as a fungal insecticide. the expression of bumblebee, bombus ignitus, venom serine protease (vsp) by beauveria bassiana (erl1170) induced melanization of yellow spotted longicorn beetles (psacothea hilaris) as an over-reactive immune response, and caused substantially earlier mortality in beet armyworm (sp ...201323626832
discriminating fever behavior in house flies.fever has generally been shown to benefit infected hosts. however, fever temperatures also carry costs. while endotherms are able to limit fever costs physiologically, the means by which behavioral thermoregulators constrain these costs are less understood. here we investigated the behavioral fever response of house flies (musca domestica l.) challenged with different doses of the fungal entomopathogen, beauveria bassiana. infected flies invoked a behavioral fever selecting the hottest temperatu ...201323620820
[antioxidation and multidrug resistance of structure-similar atp-binding cassette transporter proteins in two fungal entomopathogens].structure-similar atp-binding cassette (abc) transporter proteins, bbt1 in beauveria bassiana (bb) and ift1 in isaria fumosorosea (if), were characterized.201323614237
engineered production of fungal anticancer cyclooligomer depsipeptides in saccharomyces cerevisiae.two fungal cyclooligomer depsipeptide synthetases(codss), bbbeas (352 kda) and bbbsls (348 kda) from beauveria bassiana atcc7159, were reconstituted in saccharomyces cerevisiae bj5464-npga, leading to the production of the corresponding anticancer natural products, beauvericins and bassianolide, respectively. the titers of beauvericins (33.8 ± 1.4 mg/l) and bassianolide (21.7± 0.1 mg/l) in the engineered s. cerevisiae bj5464-npga strains were comparable to those in the native producer b. bassian ...201323608474
establishing fungal entomopathogens as endophytes: towards endophytic biological control.beauveria bassiana is a fungal entomopathogen with the ability to colonize plants endophytically. as an endophyte, b. bassiana may play a role in protecting plants from herbivory and disease. this protocol demonstrates two inoculation methods to establish b. bassiana endophytically in the common bean (phaseolus vulgaris), in preparation for subsequent evaluations of endophytic biological control. plants are grown from surface-sterilized seeds for two weeks before receiving a b. bassiana treatmen ...201323603853
differential contributions of five abc transporters to mutidrug resistance, antioxidion and virulence of beauveria bassiana, an entomopathogenic fungus.multidrug resistance (mdr) confers agrochemical compatibility to fungal cells-based mycoinsecticdes but mechanisms involved in mdr remain poorly understood for entomopathogenic fungi, which have been widely applied as biocontrol agents against arthropod pests. here we characterized the functions of five atp-binding cassette (abc) transporters, which were classified to the subfamilies abc-b (mdr1), abc-c (mrp1) and abc-g (pdr1, pdr2 and pdr5) and selected from 54 full-size abc proteins of beauver ...201323596534
effects of entomopathogenic fungus species, and impact of fertilizers, on biological control of pecan weevil (coleoptera: curculionidae).the pecan weevil, curculio caryae (horn), is a key pest of pecan, carya illinoinensis (wangenh.) k. koch. prior research indicated the potential for use of hypocreales fungi to suppress c. caryae. we compared the efficacy of two fungal spp., beauveria bassiana (gha strain) and metarhizium brunneum (f52), in their ability to cause c. caryae mortality. the fungus, b. bassiana, was applied to trunks of pecan trees (a method previously shown to be effective in c. caryae suppression) and efficacy was ...201323575015
can insects develop resistance to insect pathogenic fungi?microevolutionary adaptations and mechanisms of fungal pathogen resistance were explored in a melanic population of the greater wax moth, galleria mellonella. under constant selective pressure from the insect pathogenic fungus beauveria bassiana, 25(th) generation larvae exhibited significantly enhanced resistance, which was specific to this pathogen and not to another insect pathogenic fungus, metarhizium anisopliae. defense and stress management strategies of selected (resistant) and non-selec ...201323560083
a putative α-glucoside transporter gene bbagt1 contributes to carbohydrate utilization, growth, conidiation and virulence of filamentous entomopathogenic fungus beauveria bassiana.carbohydrate transporters are critical players mediating nutrient uptake during saprophytic and pathogenic growth for most filamentous fungi. for entomopathogenic fungi, such as beauveria bassiana, assimilation of α-glucosides, in particular, trehalose, the major carbohydrate constituent of the insect haemolymph, has been hypothesized to represent an important ability for infectious growth within the insect hemocoel. in this study, a b. bassiana α-glucoside transporter homolog was identified and ...201323499939
insight into the transcriptional regulation of msn2 required for conidiation, multi-stress responses and virulence of two entomopathogenic fungi.msn2/4 transcription factors in some fungi have null effects on virulence and cellular stress responses. here we found that the transcriptional regulation of msn2 orthologs is vital for the conidiation, virulence and multi-stress responses of beauveria bassiana (bb) and metarhizium robertsii (mr), which lack msn4 orthologs. compared to wild-type and complemented strains of each fungus with all similar phenotypes, δbbmsn2 and δmrmsn2 showed remarkable defects in conidial yield (∼40% decrease) and ...201323466345
diversity analysis of beauveria bassiana isolated from infected silkworm in southwest china based on molecular data and morphological features of colony.beauveria bassiana is an important entomopathogenic fungus that not only often causes infection and epidemics of wild insects but some strains also show pathogenicity to the silkworm, bombyx mori. the present study is about diversity of b. bassiana isolated from the silkworm in southwest china. five strains of b. bassiana were isolated from infected silkworm. two isolates, gxtr1009 and gxtr1010, were isolated from infected silkworms treated with two kinds of biological pesticides applied in guan ...201323417263
in vitro anti-allergic activity of the fungal metabolite pyridovericin.mast cells play a critical role during the development of an allergic response. upon activation by an antigen and ige, via fcεri receptors, mast cells release histamine and other mediators that initiate and propagate immediate hypersensitivity reactions. mast cells also secrete cytokines that regulate the immune responses. in this way, inhibitors of mast cell activity could work as promising therapeutics for allergic disorders. in the present work, we investigated the capacity of pyridovericin, ...201323399420
biotransformation of vulgarin.using a standard two-stage fermentation technique, the fungus beauveria bassiana (atcc 7159) was found to convert the eudesmanolide vulgarin (1) to 1α,4α-dihydroxy-5αh,6,11βh-eudesman-6,12-olide (2). the use of the yeasthansenula anomala atcc 20170 instead, produced the less polar 4α-hydroxy-1-oxo-5αh,6,11βh-eudesman-6,12-olide(3), in addition to the more polar 3α,4α-dihydroxy-1-oxo-5αh,6,11βh-eudesman-6,12-olide (4). these metabolites were characterized on the basis of their spectral data and t ...201323373652
examining the "evolution of increased competitive ability" hypothesis in response to parasites and pathogens in the invasive paper wasp polistes dominula.successful invaders often become established in new ranges by outcompeting native species. the "evolution of increased competitive ability" hypothesis predicts that invasive species are subjected to less predation and parasitization than sympatric native species, and thus can allocate resources from defence and immunity to growth and fecundity, thereby achieving higher fitness. in this study, we examined whether american invasive polistes dominula paper wasps have reduced immunocompetence. to ex ...201323354757
symbiont-mediated protection against fungal pathogens in pea aphids: a role for pathogen specificity?here we show that a bacterial endosymbiont, regiella insecticola, protects pea aphids (acyrthosiphon pisum) from the aphid-specific fungal entomopathogen zoophthora occidentalis but not from the generalist insect fungal pathogen beauveria bassiana. this finding highlights the complex influence of fungi on the dynamics of this economically important agricultural pest.201323354709
disclosing a crypt: microbial diversity and degradation activity of the microflora isolated from funeral clothes of cardinal peter pázmány.a crypt can be considered as a particular environment where different microbial communities contribute to decomposition of organic materials present inside during a long interval of time. the textile remains of the funeral clothes (biretta and tunic) of cardinal pázmány, an important historic figure dead in bratislava the 19th march 1637, conserved in this kind of environment were subjected to microbial investigation. the sampling comprised three different approaches and the use of various kinds ...201323305771
update on the status of bemisia tabaci in the uk and the use of entomopathogenic fungi within eradication programmes.the sweetpotato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) continues to be a serious threat to crops worldwide. the uk holds protected zone status against this pest and, as a result, b. tabaci entering on plant material is subjected to a policy of eradication. both b and q bemisia biotypes are now regularly intercepted entering the uk. with increasing reports of neonicotinoid resistance in both these biotypes, it is becoming more problematic to control/eradicate. therefore, alt ...201326464385
entomopathogenic fungi associated with exotic invasive insect pests in northeastern forests of the usa.mycopathogens of economically important exotic invasive insects in forests of northeastern usa have been the subject of research at the entomology research laboratory, university of vermont, for the last 20 years. elongate hemlock scale, european fruit lecanium, hemlock woolly adelgid and pear thrips were analyzed for the presence of mycopathogens, in order to consider the potential for managing these pests with biological control. fungal cultures isolated from insects with signs of fungal infec ...201326462527
selection of a fungal isolate for the control of the pink hibiscus mealybug maconellicoccus hirsutus.maconellicoccus hirsutus green is a widely distributed pest of numerous crops. although synthetic pesticides are used to control this pest, entomopathogenic fungi may provide an alternative control mechanism. three experiments were carried out to select a fungal isolate with the potential to be used as a microbial control agent. the in vitro growth of five isolates of beauveria bassiana sensu lato (bals.) vuill and metarhizium anisopliae sensu lato (metschn.) sorokín, along with three isolates o ...201323255508
p-type calcium atpase functions as a core regulator of beauveria bassiana growth, conidiation and responses to multiple stressful stimuli through cross-talk with signalling networks.p-type ca(2+) -atpase (pmr1) is a core element in calcium-calcineurin pathway and evidence for its cross-talk with other signalling pathways in filamentous fungi is of scarcity. here, we characterized the striking functions of a golgi ca(2+) -atpase (bbpmr1) in beauveria bassiana (fungal entomopathogen) by multi-phenotypic and transcriptional analyses under normal and stressful conditions. bbpmr1 inactivation caused severe defects in nutritional uptake, growth, conidiation and germination under ...201323206243
the autophagy gene bbatg5, involved in the formation of the autophagosome, contributes to cell differentiation and growth but is dispensable for pathogenesis in the entomopathogenic fungus beauveria bassiana.autophagy is a highly conserved process, representing the major eukaryotic degradative pathway of cellular components. autophagy-mediated recycling of cellular materials contributes to cell differentiation, tissue remodelling and proper development. in fungi, autophagy is required for normal growth and cell differentiation. the entomopathogenic fungus beauveria bassiana and its invertebrate targets represent a unique model system with which to examine host-pathogen interactions. the atg5 gene is ...201323197175
pathogenicity, ovicidal action, and median lethal concentrations (lc 50 ) of entomopathogenic fungi against exotic spiralling whitefly, aleurodicus dispersus russell.biological control using entomopathogenic fungi could be a promising alternative to chemical control. entomopathogenic fungi, beauveria bassiana (balsamo) vuillemin, metarhizium anisopliae (metschnikoff) sorokin, lecanicillium lecanii (zimmerm.) zare and gams, and paecilomyces fumosoroseus (wize) brown and smith, were tested for their pathogenicity, ovicidal effect, and median lethal concentrations (lc50) against exotic spiralling whitefly, aleurodicus dispersus russell. the applications were ma ...201324455279
nutritional optimization of a native beauveria bassiana isolate (hq917687) pathogenic to housefly, musca domestica l.a new beauveria bassiana isolate, showing high activity against musca domestica l. (diptera: muscidae) adults (mortality-100.0 %), larvae (mortality-72.3 %) and pupae (infection in emerged flies-96.7 %) was used. the isolate was subjected to a combinational approach towards selection of process parameters for its growth optimization. initial screening of several carbon and nitrogen sources revealed glucose and nano3 as the most suitable source for optimal biomass and spore production. further, o ...201324431571
efficacy of two fungus-based biopesticide against the honeybee ectoparasitic mite, varroa destructor.the varroa mite, varroa destructor (anderson and trueman) (acari: varroidae), is known as the most serious ectoparasitic mite on honeybee, apis mellifera (hymenoptera: apidae) in the world. based on the spores of entomopathogenic fungi, two commercial preparations; bioranza (metarhizium anisopliae) and biovar (beauveria bassiana) were evaluated through application into the hives against varroa mite. data showed significant differences between treatments with bioranza and biovar, the results were ...201324498835
laboratory assessment of beauveria bassiana (hypocreales: clavicipitaceae) strain gha for control of listronotus maculicollis (coleoptera: curculionidae) adults.bioassays were designed to evaluate beauveria bassiana (balsamo) vuillemin strain gha against listronotus maculicollis (kirby) adults. b. bassiana and its "inert" carrier oil in the product botanigard and the inert carrier oil alone provided 99 and 96% mortality, respectively, in petri dish assays 1 d after treatment when applied in 1 ml water. when the same treatments were applied in 0.5 ml of carrier water, mortality was only 1.4 and 0.7%, respectively, 1 d after treatment. after 10 d in petri ...201324498729
characterization and biocontrol potential of entomopathogenic fungus, beauveria bassiana isolates against spilarctia obliqua.beauveria bassiana is a known natural enemy of a number of insect pests of crop plants. in order to screen different isolates of any given entomopathogens molecular markers provide a means for constructing the molecular phylogeny, diversity and link to virulent phenotypes. eight isolates of b. bossiona isolated from different insect hosts and from different location at pantnagar (uttrakhand) were characterized by pcr-based rapd markers. bioassays were conducted by using first, second and third i ...201324558805
biological control of aphid using fungal culture and culture filtrates of beauveria bassiana.aphids are one of the most destructive pests in crop production such as pepper, cucumber, and eggplants. the importance of entomopathogenic fungi as alternative pest control agents is increasing. conidia of entomopathogenic fungi are influenced by environmental conditions, such as temperature and relative humidity, and cause slow and fluctuating mortality. these factors have prevented wider application and use of biocontrol agents. for investigation of means of mitigation of such problems, we co ...201324493943
[use of entomopathogenic fungi to degrade wax secreted by ceroplastes japonicus].we used entomopathogenic fungi to degrade insect wax.201323957148
a fungal insecticide engineered for fast per os killing of caterpillars has high field efficacy and safety in full-season control of cabbage insect pests.fungal insecticides developed from filamentous pathogens of insects are notorious for their slow killing action through cuticle penetration, depressing commercial interest and practical application. genetic engineering may accelerate their killing action but cause ecological risk. here we show that a beauveria bassiana formulation, hv8 (bbhv8), engineered for fast per os killing of caterpillars by an insect midgut-acting toxin (vip3aa1) overexpressed in conidia has both high field efficacy and s ...201323956386
association between entomopathogenic nematodes and fungi for control of rhipicephalus microplus (acari: ixodidae).the aim of the study was to assess the effect of the association of entomopathogenic nematodes and fungi on rhipicephalus microplus. the nematodes used were heterorhabditis bacteriophora hp88 and heterorhabditis indica lpp1 and the fungi were metarhizium anisopliae ibcb 116 and beauveria bassiana esalq 986. in the groups treated with the fungi, the females were immersed for 3 min in a conidial suspension, while in the groups treated with the nematodes, the ticks were exposed to infective juvenil ...201323949242
a putative link between phagocytosis-induced apoptosis and hemocyanin-derived phenoloxidase activation.apoptosis and phagocytosis are crucial processes required for developmental morphogenesis, pathogen deterrence and immunomodulation in metazoans. we present data showing that amebocytes of the chelicerate, limulus polyphemus, undergo phagocytosis-induced cell death after ingesting spores of the fungus, beauveria bassiana, in vitro. the observed biochemical and morphological modifications associated with dying amebocytes are congruent with the hallmarks of apoptosis, including: extracellularisati ...201323925540
efficacy of entomopathogenic hypocrealean fungi against periplaneta americana.the american cockroach periplaneta americana, one of the worlds' most important urban insect pests was tested with entomopathogenic fungi. most promising metarhizium anisopliae, metarhizium robertsii and beauveria bassiana killed nymphs (≥ 81.7% mortality, 25 days after treatment), and these fungi developed on all dead insects. other fungi tested were less virulent (metarhizium frigidum and purpureocillium lilacinum) or avirulent (isaria cateniobliqua, isaria farinosa, simplicillium lanosoniveum ...201323899866
evaluation of entomopathogenic fungi against chilli thrips, scirtothrips dorsalis.commercial strains of entomopathogenic fungi were evaluated for control of chilli thrips, scirtothrips dorsalis hood (thysanoptera: thripidae), an invasive pest of ornamental and vegetable plants in the caribbean and southeastern united states. in laboratory assays, lc50 values against adult s. dorsalis were 5.1 × 10(4) cfu/ml for beauveria bassiana gha, with higher values 3.1 × 10(5) for metarhizium brunneum f52 and 3.8 × 10(5) for isaria fumosorosea apopka 97. second instars were comparatively ...201323895429
preparation, characterization, and insecticidal activity evaluation of three different formulations of beauveria bassiana against musca domestica.three formulations; bait, encapsulation, and emulsion of beauveria bassiana were prepared and evaluated for their insecticidal activity in simulated field settings. tea waste-based bait formulation of b. bassiana showed 100% mortality (within 72 h) in lab assay against adult houseflies. in field assay using traps, 65% relative entrapment and 100 % mortality (within 60 h) of entrapped flies was observed. although the bait formulation was low cost and easy to prepare and transport, its storage abi ...201323861009
in-planta detection and monitorization of endophytic colonization by a beauveria bassiana strain using a new-developed nested and quantitative pcr-based assay and confocal laser scanning microscopy.beauveria bassiana strain 04/01-tip obtained from larvae of the opium poppy stem gall iraella luteipes endophytically colonizes opium poppy plants and protect it against this pest. development of a specific, rapid and sensitive technique that allows accurately determining the process and factors leading to the establishment of this strain in opium poppy plants would be essential to achieve its efficient control in a large field scale. for that purpose in the present study, species-specific prime ...201323851123
cloning and characterization of the gene cluster required for beauvericin biosynthesis in fusarium proliferatum.beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. we isolated and characterized the fpbeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus fusarium proliferatum lf061. targeted inactivation of the f. proliferatum genomic copy of fpbeas abolished the production of beauvericin. comparative sequence analysis of the fpbeas showed 74% similarity with the bbbeas that synthesizes the cyclic ...201323832252
metabolic effect of an exogenous gene on transgenic beauveria bassiana using liquid chromatography-mass spectrometry-based metabolomics.genetic modification of beauveria bassiana with the scorpion neurotoxin aait gene can distinctly increase its insecticidal activity, whereas the effect of this exogenous gene on the metabolism of b. bassiana is unknown until now. thus, we investigate the global metabolic profiling of mycelia and conidia of transgenic and wild-type b. bassiana by liquid chromatography-mass spectrometry (lc-ms). principal component analysis (pca) and orthogonal projection to latent structure discriminant analysis ...201323822565
a carbon responsive g-protein coupled receptor modulates broad developmental and genetic networks in the entomopathogenic fungus, beauveria bassiana.in fungi, g-protein coupled receptors (gpcrs) link ligand/nutrient sensing to growth, mating, developmental/life-stage activation and pathogenesis. a gpcr was characterized from the entomopathogenic fungus, beauveria bassiana (bbgpcr3), which links nutrient sensing to stress response and development. δbbgpcr3 mutants grew slower on various carbohydrates and displayed increased sensitivity to osmotic, oxidative and cell wall stresses. gene expression profiling revealed a set of heat-shock and ant ...201323809710
distribution of the entomopathogenic fungus beauveria bassiana in rice ecosystems and its effect on soil enzymes.fungal entomopathogens, especially beauveria bassiana, are often studied within the context of their use in biological pest control; however, there is limited knowledge of their distributions in host plants and soil ecosystem. we examined the distribution of b. bassiana and its influence on rice plants and paddy soils. b. bassiana could only be detected on the foliar surfaces of rice plants within 15 days under bb-4 (7.5 × 10(4) conidia/ml) and bb-7 (7.5 × 10(7) conidia/ml) treatments. the endop ...201323794015
lactones 42. stereoselective enzymatic/microbial synthesis of optically active isomers of whisky lactone.two different methods, enzyme-mediated reactions and biotrasformations with microorganisms, were applied to obtain optically pure cis- and trans-isomers of whisky lactone 4a and 4b. in the first method, eight alcohol dehydrogenases were investigated as biocatalysts to enantioselective oxidation of racemic erythro- and threo-3-methyloctane-1,4-diols (1a and 1b). oxidation processes with three of them, alcohol dehydrogenases isolated from horse liver (hladh) as well as recombinant from escherichia ...201323768375
effects on diversity of soil fungal community and fate of an artificially applied beauveria bassiana strain assessed through 454 pyrosequencing.the entomopathogenic fungus beauveria bassiana is widely used as a biological control agent (bca) for insect pest control, with fungal propagules being either incorporated into the potting media or soil or sprayed directly onto the foliage or soil. to gain a better understanding of entomopathogenic fungal ecology when applied as a bca to the soil environment, a case study using tag-encoded 454 pyrosequencing of fungal its sequences was performed to assess the fate and potential effect of an arti ...201323736813
pseudomonas reactans, a bacterial strain isolated from the intestinal flora of blattella germanica with anti-beauveria bassiana activity.anti-beauveria bassiana activity of aqueous fecal extracts from conventional german cockroaches [blattella germanica (l.)] was detected, but was not detected in samples from germ-free german cockroaches. subsequently, bacterial strain bgi-14 was isolated from the gut of conventional german cockroaches and was identified as pseudomonas reactans based on 16s rdna sequence. the strain bgi-14 not only inhibited the germination of conidia, but also inhibited the growth of b. bassiana hyphae. further ...201323726054
activity of fusion prophenoloxidase-gfp and its potential applications for innate immunity study.insect prophenoloxidase (ppo) is essential for physiological functions such as melanization of invading pathogens, wound healing and cuticle sclerotization. the insect ppo activation pathway is well understood. however, it is not very clear how ppo is released from hemocytes and how ppo takes part in cellular immunity. to begin to assess this, three drosophila melanogaster ppo genes were separately fused with gfp at the c-terminus (rppo-gfp) and were over-expressed in s2 cells. the results of st ...201323717543
differentially expressed genes in the cuticle and hemolymph of the silkworm, bombyx mori, injected with the fungus beauveria bassiana.the most important pathogenic fungus of the silkworm, bombyx mori l. (lepidoptera: bombycidae), is beauveria bassiana (balsamo-crivelli ) vuillemin (hypocreales: clavicipitaceae), which causes significant damage to sericulture production. therefore, diagnosing fungal disease and developing new control measures are crucial to silk production. to better understand the responsive and interactive mechanisms between the host silkworm and this fungus, variations in silkworm gene expression were invest ...201324794288
mycosis inhibits cannibalism by melanoplus sanguinipes, m. differentialis, schistocerca americana, and anabrus simplex.cannibalism is common among the acrididae and the mormon cricket, anabrus simplex haldeman (orthoptera: tettigoniidae). this behavior has been proposed as a mechanism for the horizontal transmission of microsporida and entomopathogenic fungi. aanecdotal observations suggested that the migratory grasshopper, melanoplus sanguinipes fabricius (acrididae), and a. simplex did not eat cadavers that had been killed by insect pathogenic fungi. the hypothesis tested was that a. simplex or m. sanguinipes ...201324786183
recognition of a core fragment ofbeauveria bassiana hydrophobin gene promoter (p hyd1) and its special use in improving fungal biocontrol potential.to identify a suitable promoter for use in engineering fungal entomopathogens to improve heterologous gene expression and fungal biocontrol potential, a 1798 bp promoter (p hyd1) upstream of beauveria bassiana class i hydrophobin gene (hyd1) was optimized by upstream truncation and site-directed mutation. a truncated 1290 bp fragment (p hyd1-t1) drove egfp expression in b. bassiana much more efficiently than full-length p hyd1. further truncating p hyd1-t1 to 1179, 991 and 791 bp or mutating one ...201322639846
differentiated functions of ras1 and ras2 proteins in regulating the germination, growth, conidiation, multi-stress tolerance and virulence of beauveria bassiana.ras1 and ras2 are two distinct ras gtpases in beauveria bassiana, an entomopathogenic fungus whose biocontrol potential against insect pests depends largely on virulence and multi-stress tolerance. the functions of both proteins were characterized for the first time by constructing dominant-active (gtp-bound) ras1(g19v) and dominant-negative (gdp-bound) ras1(d126a) and integrating them and normal ras1 into wild type and δras2 for a series of phenotypic and transcriptional analyses. the resultant ...201322958161
microbial metabolism. part 14. isolation and bioactivity evaluation of microbial metabolites of resveratrol.the fungi, beauveria bassiana (atcc 13144) and penicillium chrysogenium (atcc 9480) transformed resveratrol to resveratrol-3-o-sulphate. the former, in addition, gave 5-methoxyresveratrol-3-o-β-glucoside with the latter yielding 5-methoxyresveratrol-3-o-sulphate. the structures were established by spectroscopic methods. evaluation of biological activity of metabolites through a series of mammalian cell based assays indicated that resveratrol tends to lose its anti-inflammatory, cytotoxic and ant ...201322950854
catalases play differentiated roles in the adaptation of a fungal entomopathogen to environmental stresses.the catalase family of beauveria bassiana (fungal entomopathogen) consists of cata (spore-specific), catb (secreted), catp (peroxisomal), catc (cytoplasmic) and catd (secreted peroxidase/catalase), which were distinguished in phylogeny and structure and functionally characterized by constructing single-gene disrupted and rescued mutants for enzymatic and multi-phenotypic analyses. total catalase activity decreased 89% and 56% in δcatb and δcatp, corresponding to the losses of upper and lower act ...201322891860
morphological and ultrastructural characterization of carposina sasakii larvae (lepidoptera: carposinidae) infected by beauveria bassiana (ascomycota: hypocreales: clavicipitaceae).the aim of this study was to better understand the pathogenesis of the entomopathogenic fungus beauveria bassiana (balsamo) strain tst05 observed on the peach fruit moth (carposina sasakii (matsumura)), an important orchard pest. the morphological and ultrastructural characterization of the mature larvae of c. sasakii infected by b. bassiana was investigated by using light, scanning and transmission electron microscopy. the results of the study show that b. bassiana tst05 infected the host larva ...201322940571
morphological changes, chitinolytic enzymes and hydrophobin-like proteins as responses of lecanicillium lecanii during growth with hydrocarbon.lecanicillium lecanii, verticillium chlamydosporium, v. fungicola var flavidum and beauveria bassiana were evaluated on their growth with pure n-hexane, toluene and n-hexane:toluene 17:83 (v:v) mixture. another set of treatments were conducted with colloidal chitin as additional carbon source. all the strains of lecanicillium were able to grow using hydrocarbons with or without the addition of chitin, although the presence of hydrocarbons showed significant inhibition evidenced by measured bioma ...201322926786
antimicrobial activity of a honeybee (apis cerana) venom kazal-type serine protease inhibitor.insect-derived kazal-type serine protease inhibitors exhibit thrombin, elastase, plasmin, proteinase k, or subtilisin a inhibition activity, but so far, no functional roles for bee-derived kazal-type serine protease inhibitors have been identified. in this study, a bee (apis cerana) venom kazal-type serine protease inhibitor (acktspi) that acts as a microbial serine protease inhibitor was identified. acktspi contained a single kazal domain that displayed six conserved cysteine residues and a p1 ...201324076031
the identification of a bacterial strain bgi-1 isolated from the intestinal flora of blattella germanica, and its anti-entomopathogenic fungi activity.a bacterial strain bgi-1 was isolated from the gut of german cockroaches (blattella germanica l.) and was identified as bacillus subtilis based on 16s rdna sequence and morphological, physiological, and biochemical characters. the strain bgi-1 inhibited the growth of beauveria bassiana; the diameter of the inhibition zone exceeded 30 mm. vesicles were observed in b. bassiana hyphae on the edge of the inhibition zone. fermentation of bgi-1 reduced the conidial germination rate by 12%. further stu ...201323448013
combined effect of microbial and chemical control agents on subterranean termites.termite mortality was measured when fungi were combined with bacteria or a chemical termiticide to determine whether a synergistic effect occurred. the fungus beauveria bassiana was combined with the non-repellant chemical termiticide imidacloprid. of the three b. bassiana strains tested one, b. bassiana atcc 90519, was sufficiently pathogenic on its own that the advantage of a supplementary chemical treatment was marginal. the mortality caused by another fungal strain, b. bassiana atcc 26037, w ...201324037651
developmental regulation and antifungal activity of a growth-blocking peptide from the beet armyworm spodoptera exigua.insect cytokine growth-blocking peptides (gbps) are involved in growth regulation and the innate immune response. however, the microbial binding and antimicrobial activities of gbps remain unclear. here, we investigate the developmental role and antifungal activity of a gbp from the beet armyworm spodoptera exigua (segbp). sequence analysis predicted that mature segbp consists of 24 amino acid residues, including 2 cysteine residues. during s. exigua development, segbp is constitutively expresse ...201323732405
investigating alternatives to traditional insecticides: effectiveness of entomopathogenic fungi and bacillus thuringiensis against citrus thrips and avocado thrips (thysanoptera: thripidae).citrus thrips, scirtothrips citri (moulton) (thysanoptera: thripidae), is a plant-feeding pest most widely recognized for causing damage to citrus (citrus spp. l. [rutaceae]) and mango (mangifera indica l. [anacardiaceae]) fruits. this insect has recently broadened its known host range to become a significant pest of california grown blueberries. avocado thrips, scirtothrips. perseae nakahara (thysanoptera: thripidae), is a recent, invasive pest of california avocados, persea americana mill. (la ...201323448016
ochratoxin a management in vineyards by lobesia botrana biocontrol.grape berries attacked by lobesia botrana larvae are more easily infected by aspergillus section nigri (black aspergilli) ochratoxigenic species. two-year field trials were carried out in apulia (italy) to evaluate a bioinsecticide control strategy against l. botrana and the indirect effect on reducing ochratoxin a (ota) contamination in vineyards. a commercial bacillus thuringiensis formulate and an experimental beauveria bassiana (item-1559) formulate were tested in two vineyards cultivated wi ...201323282670
immunosuppressive properties of a protein (rvpr1) from the venom of the endoparasitic wasp, pimpla hypochondriaca: mechanism of action and potential use for improving biological control strategies.previously, it was determined that the presence of rvpr1 (a recombinant pimpla hypochondriaca venom protein), in the haemocoel of two lepidopteran larvae, significantly increases their susceptibility to the biological control agents (bcas), bacillus thuringiensis (bt) and beauveria bassiana (richards and dani, 2010; richards et al., 2011). the current work examines the mechanism of action of rvpr1 and demonstrates that it binds to the surface of some haemocytes and disrupts the organization of t ...201322698823
leaf microbiota of strawberries as affected by biological control agents.the increasing use of biological control agents (bcas) against botrytis cinerea in strawberry raises the question of whether there are any undesirable impacts of foliar applications of bcas on nontarget microorganisms in the phyllosphere. therefore, our objective was to investigate this issue within a field study. strawberry plants were repeatedly sprayed with three bcas-namely, rhizovital 42 fl. (bacillus amyloliquefaciens fzb42), trianum-p (trichoderma harzianum t22), and naturalis (beauveria ...201324020904
occurrence and prevalence of insect pathogens in populations of the codling moth, cydia pomonella l.: a long-term diagnostic survey.about 20,550 larvae, pupae and adults of the codling moth, cydia pomonella l., were diagnosed for pathogens during long-term investigations (1955-2012) at the institute for biological control in darmstadt, germany. the prevailing entomopathogens diagnosed in these studies were insect pathogenic fungi, especially beauveria bassiana and isaria farinosa, the microsporidium, nosema carpocapsae, the cydia pomonella granulovirus (cpgv), as well as mostly undetermined bacteria. while the cpgv was obser ...201326462428
plant and fungal diversity in gut microbiota as revealed by molecular and culture investigations.few studies describing eukaryotic communities in the human gut microbiota have been published. the objective of this study was to investigate comprehensively the repertoire of plant and fungal species in the gut microbiota of an obese patient.201323555039
identification and expression profile analysis of antimicrobial peptide/protein in asian corn borer, ostrinia furnacalis (guenée).antimicrobial peptides/proteins (amps) are a group of immune proteins that exhibit strong antibiotic properties against numerous infectious bacterial strains. they are evolutionarily conserved and present in every kingdom and phylum, ranging from prokaryotes to humans. we analyzed the transciptome from the larvae of asian corn borer, ostrinia furnacalis (guenée), and identified several putative amp transcripts, ofglys5, ofglys6, ofglys10, ofgatt, and ofgiid. ofglys5, ofglys6, and ofglys10 are al ...201324155672
characterizations and functions of regulator of g protein signaling (rgs) in fungi.proteins that serve as regulator of g protein signaling (rgs) primarily function as gtpase accelerators that promote gtp hydrolysis by the gα subunits, thereby inactivating the g protein and rapidly switching off g protein-coupled signaling pathways. since the first rgs protein was identified from the budding yeast saccharomyces cerevisiae, more than 30 rgs and rgs-like proteins have been characterized from several model fungi, such as aspergillus nidulans, beauveria bassiana, candida albicans, ...201323917634
analysis of whitefly transcriptional responses to beauveria bassiana infection reveals new insights into insect-fungus interactions.the fungal pathogen, beauveria bassiana, is an efficient biocontrol agent against a variety of agricultural pests. a thorough understanding of the basic principles of insect-fungus interactions may enable the genetic modification of beauveria bassiana to enhance its virulence. however, the molecular mechanism of insect response to beauveria bassiana infection is poorly understood, let alone the identification of fungal virulent factors involved in pathogenesis.201323861870
immune-related transcriptome of coptotermes formosanus shiraki workers: the defense mechanism.formosan subterranean termites, coptotermes formosanus shiraki, live socially in microbial-rich habitats. to understand the molecular mechanism by which termites combat pathogenic microbes, a full-length normalized cdna library and four suppression subtractive hybridization (ssh) libraries were constructed from termite workers infected with entomopathogenic fungi (metarhizium anisopliae and beauveria bassiana), gram-positive bacillus thuringiensis and gram-negative escherichia coli, and the libr ...201323874972
targeting of insect epicuticular lipids by the entomopathogenic fungus beauveria bassiana: hydrocarbon oxidation within the context of a host-pathogen interaction.broad host range entomopathogenic fungi such as beauveria bassiana attack insect hosts via attachment to cuticular substrata and the production of enzymes for the degradation and penetration of insect cuticle. the outermost epicuticular layer consists of a complex mixture of non-polar lipids including hydrocarbons, fatty acids, and wax esters. long chain hydrocarbons are major components of the outer waxy layer of diverse insect species, where they serve to protect against desiccation and microb ...201323422735
expression of trypsin modulating oostatic factor (tmof) in an entomopathogenic fungus increases its virulence towards anopheles gambiae and reduces fecundity in the target mosquito.adult and larval mosquitoes regulate food digestion in their gut with trypsin modulating oostatic factor (tmof), a decapeptide hormone synthesized by the ovaries and the neuroendocrine system. tmof is currently being developed as a mosquitocide, however, delivery of the peptide to the mosquito remains a significant challenge. entomopathogenic fungi offer a means for targeting mosquitoes with tmof.201323336669
effect of temperature and humidity on pathogenicity of native beauveria bassiana isolate against musca domestica l.beauveria bassiana hq917687 virulence to housefly larvae and adult was assessed at different relative humidity, rh (50, 75, 90, and 100 %) and temperature (15, 20, 25, 30, 35, 40, 45 °c) conditions at the fungal dose of 10(8) conidia/ml. depending on the temperature and rh regime tested, difference in mortality rates of housefly adult and larvae were detected. during assay on adult housefly, 100 % mortality was achieved at rh, 90 and 100 % while the temperature of 30 °c showed maximum mortality ...201326688637
malaria mosquitoes attracted by fatal fungus.insect-killing fungi such as beauveria bassiana are being evaluated as possible active ingredients for use in novel biopesticides against mosquito vectors that transmit malaria. fungal pathogens infect through contact and so applications of spores to surfaces such as walls, nets, or other resting sites provide possible routes to infect mosquitoes in and around domestic dwellings. however, some insects can detect and actively avoid fungal spores to reduce infection risk. if true for mosquitoes, s ...201323658757
identification of immunity-related genes in ostrinia furnacalis against entomopathogenic fungi by rna-seq analysis.the asian corn borer (ostrinia furnacalis (guenée)) is one of the most serious corn pests in asia. control of this pest with entomopathogenic fungus beauveria bassiana has been proposed. however, the molecular mechanisms involved in the interactions between o. furnacalis and b. bassiana are unclear, especially under the conditions that the genomic information of o. furnacalis is currently unavailable. so we sequenced and characterized the transcriptome of o. furnacalis larvae infected by b. bass ...201424466095
natural variation in virulence of the entomopathogenic fungus beauveria bassiana against malaria mosquitoes.insecticide resistance is greatly hampering current efforts to control malaria and therefore alternative methods are needed. entomopathogenic fungi have been proposed as an alternative with a special focus on the cosmopolitan species beauveria bassiana. however, few studies have analysed the effects of natural variation within fungal isolates on mosquito survival, and the implications and possible exploitation for malaria control.201425480526
development and evaluation of a novel contamination device that targets multiple life-stages of aedes aegypti.the increasing global threat of dengue demands new and easily applicable vector control methods. ovitraps provide a low-tech and inexpensive means to combat dengue vectors. here we describe the development and optimization process of a novel contamination device that targets multiple life-stages of the aedes aegypti mosquito. special focus is directed to the diverse array of control agents deployed in this trap, covering adulticidal, larvicidal and autodissemination impacts.201424766772
a serine protease homolog negatively regulates tep1 consumption in systemic infections of the malaria vector anopheles gambiae.clip domain serine protease homologs are widely distributed in insect genomes and play important roles in regulating insect immune responses, yet their exact functions remain poorly understood. here, we show that clipa2, a clip domain serine protease homolog of anopheles gambiae, regulates the consumption of the mosquito complement-like protein tep1 during systemic bacterial infections. we provide evidence that clipa2 localizes to microbial surfaces in a tep1-dependent manner whereby it negative ...201425012124
reduced survival and reproductive success generates selection pressure for the dengue mosquito aedes aegypti to evolve resistance against infection by the microsporidian parasite vavraia culicis.the success and sustainability of control measures aimed at reducing the transmission of mosquito-borne diseases will depend on how they influence the fitness of mosquitoes in targeted populations. we investigated the effects of the microsporidian parasite vavraia culicis on the survival, blood-feeding behaviour and reproductive success of female aedes aegypti mosquitoes, the main vector of dengue. infection reduced survival to adulthood and increased adult female mosquito age-dependent mortalit ...201424822081
evaluating the efficacy of biological and conventional insecticides with the new 'mcd bottle' bioassay.control of mosquitoes requires the ability to evaluate new insecticides and to monitor resistance to existing insecticides. monitoring tools should be flexible and low cost so that they can be deployed in remote, resource poor areas. ideally, a bioassay should be able to simulate transient contact between mosquitoes and insecticides, and it should allow for excito-repellency and avoidance behaviour in mosquitoes. presented here is a new bioassay, which has been designed to meet these criteria. t ...201425515850
diversity of soil fungi in north 24 parganas and their antagonistic potential against leucinodes orbonalis guen. (shoot and fruit borer of brinjal).soil samples were collected from agricultural fields and gardens in north 24 parganas, west bengal, and fungi species were isolated from them. thirty-one fungal species were isolated with 19 found in agricultural soil and 28 in garden soil. twenty-eight out of 31 were identified using cultural and microscopic characters, and three were unidentified. the diversity of isolated fungi was calculated by simpson's diversity index. the garden soil possessed more fungal colonies (750) than agricultural ...201425213561
transformation of saturated nitrogen-containing heterocyclic compounds by microorganisms.the saturated nitrogen-containing heterocyclic compounds include many drugs and compounds that may be used as synthons for the synthesis of other pharmacologically active substances. the need for new derivatives of saturated nitrogen-containing heterocycles for organic synthesis, biotechnology and the pharmaceutical industry, including optically active derivatives, has increased interest in microbial synthesis. this review provides an overview of microbial technologies that can be valuable to pr ...201424352731
[antagonism against beauveria bassiana by lipopeptide metabolites produced by entophyte bacillus amyloliquefaciens strain swb16].we screened bacterial strains that have strong antagonism against beauveria bassiana, an important pathogen of silkworm industry, and detected the antagonistic activity of lipopeptide metabolites.201425252459
occurrence of fruiting structures allows determination of purpureocillium lilacinum as an inciting agent of pleuritis and pneumonia in a loggerhead sea turtle (caretta caretta) by histopathologic correlation to culture.purpureocillium lilacinum and beauveria bassiana were isolated from lung sampled at necropsy of a 12 year-old female loggerhead sea turtle (caretta caretta) that had displayed abnormal buoyancy. histopathologic evaluation revealed pleuritis and pneumonia with non-melanized, septate hyphae and fruiting structures identical to those of p. lilacinum. this case emphasizes the importance of a histological correlate to fungal culture when environmental fungi are isolated and demonstrates the infrequen ...201425379399
combination of endophytic bacillus and beauveria for the management of fusarium wilt and fruit borer in tomato.most of the approaches for biocontrol of pests and diseases have used a single biocontrol agent as antagonist to a single pest or pathogen. this accounts for the inconsistency in the performance of biocontrol agents. the development of a bioformulation possessing a mixture of bioagents could be a viable option for the management of major pests and diseases in crop plants.201424376014
a bumblebee (bombus ignitus) venom serine protease inhibitor that acts as a microbial serine protease inhibitor.serine protease inhibitors from bumblebee venom have been shown to block plasmin activity. in this study, we identified the protein bivspi from the venom of bombus ignitus to be a serine protease inhibitor and an antimicrobial factor. bivspi is a 55-amino acid mature peptide with ten conserved cysteine residues and a p1 methionine residue. bivspi is expressed in the venom gland and also found in the venom as an 8-kda peptide. recombinant bivspi that was expressed in baculovirus-infected insect c ...201424158004
dual function of a bee (apis cerana) inhibitor cysteine knot peptide that acts as an antifungal peptide and insecticidal venom toxin.inhibitor cysteine knot (ick) peptides exhibit ion channel blocking, insecticidal, and antimicrobial activities, but currently, no functional roles for bee-derived ick peptides have been identified. in this study, a bee (apis cerana) ick peptide (acick) that acts as an antifungal peptide and as an insecticidal venom toxin was identified. acick contains an ick fold that is expressed in the epidermis, fat body, or venom gland and is present as a 6.6-kda peptide in bee venom. recombinant acick pept ...201425106915
direct plantlet inoculation with soil or insect-associated fungi may control cabbage root fly maggots.a potential delia radicum biological control strategy involving cauliflower plantlet inoculation with various fungi was investigated in a series of laboratory and glasshouse experiments. in addition to entomopathogenic fungi, fungi with a high rhizosphere competence and fungi with the ability to survive as saprotrophs in soil were tested. the following fungal species were evaluated in the experiments: trichoderma atroviride, t. koningiopsis, t. gamsii, beauveria bassiana, metharhizium anisopliae ...201424907449
effects of entomopathogens on mortality of western corn rootworm (coleoptera: chrysomelidae) and fitness costs of resistance to cry3bb1 maize.fitness costs can delay pest resistance to crops that produce insecticidal toxins derived from the bacterium bacillus thuringiensis (bt), and past research has found that entomopathogens impose fitness costs of bt resistance. in addition, entomopathogens can be used for integrated pest management by providing biological control of pests. the western corn rootworm, diabrotica virgifera virgifera leconte (coleoptera: chrysomelidae), is a major pest of maize and is currently managed by planting of ...201424665720
[species diversity and temporal niche of entomopathogenic fungi in the extensively managed tea plantation soil].the species diversity and temporal niche of entomopathogenic fungi community in the rhizosphere soil collected from the extensively managed huangshan fuzz tip tea plantation were investigated. a total of 140 soil samples were collected at the location of tangkou town, huangshan of anhui province during august, 2012 to june, 2013, and totally 1041 fungal isolates were obtained on selective medium with soil dilution plating. the results showed that the entomopathogenic fungi community in the tea p ...201425898633
[identification of spore germination and virulence related biomarkers from beauveria bassiana using an lc-ms-based metabolomic technique].to identify biomarkers associated with germination and virulence of beauveria bassiana.201424783852
sustainable management tactics for control of phyllotreta cruciferae (coleoptera: chrysomelidae) on canola in montana.the crucifer flea beetle, phyllotreta cruciferae (goeze) (coleoptera: chrysomelidae), has recently emerged as a serious pest of canola (brassica napus l.) in montana. the adult beetles feed on canola leaves, causing many small holes that stunt growth and reduce yield. in 2013, damage to canola seedlings was high (approximately 80%) in many parts of montana, evidence that when flea beetles emerge in large numbers, they can quickly destroy a young canola crop. in the current study, the effectivene ...201424772547
a novel approach: beauveria bassiana granules applied to nursery soil for management of rice water weevils in paddy fields.spraying of entomopathogenic fungi formulated as wettable powders and suspension concentrates based on oils or water has been used for the management of crop pests. however, the use of fungal granules to control paddy field pests has not been fully explored. herein, several beauveria bassiana isolates (erl1170, erl1578 and erl836) were produced as granules using a millet-based solid culture. the granules were applied to the rice nursery 3 days before transplanting, and their control efficacy aga ...201424771507
specific diversity of the entomopathogenic fungi beauveria and metarhizium in mexican agricultural soils.prior knowledge of the local population structure of entomopathogenic fungi is considered an important requisite when developing microbial control strategies against major pests of crops such as white grubs. an extensive survey in the estate of guanajuato, one of the main agricultural regions of mexico, was carried out to determine the abundance and diversity of entomopathogenic fungi in soil. soil collected from 11 locations was baited for entomopathogenic fungi using galleria mellonella. in ad ...201424769124
adenylate cyclase orthologues in two filamentous entomopathogens contribute differentially to growth, conidiation, pathogenicity, and multistress responses.adenylate cyclase (ac) is a core element of camp signalling network. here we show functional diversity and differentiation of beauveria bassiana ac (bbac) and metarhizium robertsii ac (mrac). severe growth defects occurred in δbbac and δmrac grown on nutrition-rich sday and several minimal media but were largely alleviated by adding camp to sday. conidial yield increased greatly in δbbac but decreased in δmrac. during colony growth, δbbac was highly sensitive to oxidation, high osmolarity, cell ...201424742837
the connection of protein o-mannosyltransferase family to the biocontrol potential of beauveria bassiana, a fungal entomopathogen.o-mannosylation dependent on the protein o-mannosyltransferase (pmt) family is an essential post-translational modification process in eukaryotes, but their connection to the biocontrol potential of a filamentous entomopathogen against arthropod pests is not understood. here, we characterized the functions of three pmt orthologues (pmt1, pmt2 and pmt4) in the pmt family of beauveria bassiana and found that they were positive, but differential, regulators of the fungal growth, conidiation, multi- ...201424727441
phytochrome controls conidiation in response to red/far-red light and daylight length and regulates multistress tolerance in beauveria bassiana.phytochromes (phy) in filamentous fungi are group viii histidine kinases that share a unique n-terminal photosensory core, but their functions are largely unknown. here we show that beauveria bassiana phy (bbphy) is functionally vital for growth, conidiation and multistress tolerance of the fungal entomopathogen lacking sexual stage. colony growth of δbbphy was significantly slower in a nutrition-rich medium but faster in several minimal media. conidial yield of δbbphy in the rich medium increas ...201424725588
host-to-pathogen gene transfer facilitated infection of insects by a pathogenic fungus.metarhizium robertsii is a plant root colonizing fungus that is also an insect pathogen. its entomopathogenicity is a characteristic that was acquired during evolution from a plant endophyte ancestor. this transition provides a novel perspective on how new functional mechanisms important for host switching and virulence have evolved. from a random t-dna insertion library, we obtained a pathogenicity defective mutant that resulted from the disruption of a sterol carrier gene (mr-npc2a). phylogene ...201424722668
vasorelaxant activity of 7-β-o-glycosides biosynthesized from flavonoids.in this work we report the vasorelaxant activity of 7-β-o-glycosides obtained with biosynthesis of naringenin-7-β-o-glycoside (3) and quercetin-7-β-o-glycoside (4). these compounds were obtained from naringenin (1) and quercetin (2) glycosylation catalyzed by beauveria bassiana atcc 7159. screening of the best strain as a catalyst for glycosylation was carried out and the reaction conditions established. cultures were grown in pdsm medium for 7 days at 27 °c. after purification by reverse-phase ...201424704375
fitness consequences of larval exposure to beauveria bassiana on adults of the malaria vector anopheles stephensi.entomopathogenic fungi have shown to be effective in biological control of both larval and adult stages of malaria mosquitoes. however, a small fraction of mosquitoes is still able to emerge after treatment with fungus during the larval stage. it remains unclear whether fitness of these adults is affected by the treatment during the larval stage and whether they are still susceptible for another treatment during the adult stage. therefore, we tested the effects of larval exposure to the entomopa ...201424694552
establishment of fungal entomopathogens beauveria bassiana and bionectria ochroleuca (ascomycota: hypocreales) as endophytes on artichoke cynara scolymus.entomopathogenic fungi (epf) are commonly found in diverse habitats and are known to cause mycoses in many different taxa of arthropods. various unexpected roles have been recently reported for fungal entomopathogens, including their presence as fungal endophytes, plant disease antagonists, rhizosphere colonizers and plant growth promoting fungi. in tunisia, a wide range of indigenous epf isolates from different species, such as beauveria bassiana and bionectria ochroleuca, were found to occur i ...201424681358
physiological aspects of the interaction of multi-walled carbon nanotubes with beauveria bassiana (balsamo) vuillem in entomopathogenic spores.the objective of the study was to evaluate the in vitro effect of aqueous suspensions of commercial multi-walled carbon nanotubes (mwcnts) on spores of three entomopathogenic fungus species of beauveria bassiana. spores were placed in contact with mwcnts for 1, 24, 72 and 216 h and then an in vitro evaluation in the subculture was performed on the linear growth of the vegetative mycelium of the spores after the contact with nanomaterial, as well as its sporulation after completion of the culture ...201424679095
bbssk1, a response regulator required for conidiation, multi-stress tolerance, and virulence of beauveria bassiana.ssk1-type response regulator proteins are the core elements of histidine-to-aspartate systems that mediate fungal stress tolerance, a determinant to the biocontrol potential of fungal entomopathogens. we characterized the functions of beauveria bassiana ssk1 (bbssk1) by analyzing multi-phenotypic changes in δbbssk1 and differentially expressed genes in the digital gene expression (dge) libraries of δbbssk1 and wild-type constructed under osmotic stress. the bbssk1 disruption caused 25 % reductio ...201424633371
Refresh
firstprev
12345678910...
nextlast
Displaying items 801 - 900 of 1213