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identifying sequences potentially related to resistance response of piper tuberculatum to fusarium solani f. sp. piperis by suppression subtractive hybridization.piper tuberculatum is an exotic piper from the amazon region that shows resistance to infection by fusarium solani f. sp. piperis, causal agent of fusarium disease in black pepper (piper nigrum l.). in this work we aimed to study the interaction between p. tuberculatum and f. solani f. sp. piperis at a molecular level, using suppression subtractive hybridization to identify genes potentially related to fusarium disease resistance. comparative sequence analysis confirmed that clones isolated here ...200920001904
silver enhances the in vitro antifungal activity of the saponin, cay-1.summary the fungicidal properties of purified cay-1, dissolved silver ion and ethylenediamine tetraacetic acid (edta) separately were studied in vitro as were the abilities of silver and edta to enhance cay-1 fungicidal properties. non-germinated and germinating conidia of aspergillus flavus, aspergillus fumigatus, aspergillus niger, fusarium verticillioides (fusarium moniliforme), fusarium oxysporum and fusarium solani were incubated separately with cay-1 (0-24.8 mug ml(-1)), silver (0-111.1 mu ...200920002309
concurrent acanthamoeba and fusarium keratitis with silicone hydrogel contact lens use.purpose:: to report a case of simultaneous acanthamoeba and fusarium keratitis associated with no-rob multipurpose contact lens solution and silicone hydrogel contact lens use. method:: observational case report results:: a 39-ycar-old woman was referred for worsening of a presumed bacterial conical ulcer in the setting of silicone hydrogel lens wear with occasional overnight wear, no-rub multipurpose contact lens solution use, and combined topical antibiotic/corticosteroid treatment. initial co ...200920023585
persisting onychomycosis caused by fusarium solani in an immunocompetent patient.we present a patient with a fusarium solani infection of both distal thumbnails. she suffered from basedow's disease but had no signs of reduced immunity. the patient declined antimycotic treatment and the infection was still verifiable after 5 years. our observation shows that a superficial f. solani infection may persist in immunocompetent patients without causing noteworthy discomfort. because a superficial f. solani infection may give origin to a systemic infection it should always be treate ...200918643888
synthetic affinity ligands as a novel tool to improve protein stability.cutinase from fusarium solani pisi is the model-system for a new approach to assess and enhance protein stability based on the use of synthetic triazine-scaffolded affinity ligands as a novel protein-stabilizing tool. the active site of cutinase is excluded from the main surface regions postulated to be involved in early protein's thermal unfolding events. hence, these regions are suitable targets for binding complementary affinity ligands with a potential stabilizing effect. a random solid-phas ...200918654989
isolation and biochemical characterization of a novel leguminous defense peptide with antifungal and antiproliferative potency.leguminous plants have formed a popular subject of research owing to the abundance of proteins and peptides with important biological activities that they produce. the antifungal proteins and peptides have been purified from a number of leguminous species. however, research continues to discover novel antifungal plant-produced peptides and proteins are being needed, specially those novel ones with both antifungal activity and other significant bioactivities. the objective of this study was to is ...200918841359
bacteria and free-living amoeba in the lascaux cave.the lascaux cave was discovered in 1940, and by 1960 it had received up to 1800 daily visitors. in 1963, the cave was closed and in 2001 it was invaded by a fusarium solani species complex which was treated for four years with benzalkonium chloride. however, lascaux cave bacteria have only been poorly investigated. here we show that the cave is now a reservoir of potential pathogenic bacteria and protozoa which can be found in outbreaks linked to air-conditioning systems and cooling towers in co ...200918977432
catalysis by glomerella cingulata cutinase requires conformational cycling between the active and inactive states of its catalytic triad.cutinase belongs to a group of enzymes that catalyze the hydrolysis of esters and triglycerides. structural studies on the enzyme from fusarium solani have revealed the presence of a classic catalytic triad that has been implicated in the enzyme's mechanism. we have solved the crystal structure of glomerella cingulata cutinase in the absence and in the presence of the inhibitors e600 (diethyl p-nitrophenyl phosphate) and petfp (3-phenethylthio-1,1,1-trifluoropropan-2-one) to resolutions between ...200918983850
thctf1 transcription factor of trichoderma harzianum is involved in 6-pentyl-2h-pyran-2-one production and antifungal activity.we describe the cloning and characterization of the trichoderma harzianum thctf1 gene, which shows high sequence identity with a transcription factor gene of fusarium solani f. sp. pisi. in t. harzianum, disruption of the thctf1 gene by homologous recombination gave rise to transformants that in plate experiments did not show the yellow pigmentation observed in the wild-type strain. in several trichoderma spp. a yellow pigmentation and a coconut aroma have been related to the production of 6-pen ...200919007898
potential role of macrophages in experimental keratomycosis.to investigate the potential role of macrophages in keratomycosis.200919074808
overexpression of the chitosanase gene in fusarium solani via agrobacterium tumefaciens-mediated transformation.to overexpress the chitosanase gene (csn) in f. solani, a vector based on pcambia 1300 was constructed. the csn gene, which is under control of the aspergillus nidulans gpda promoter and a. nidulans trpc terminator, was introduced back into the f. solani genome by agrobacterium tumefaciens-mediated transformation, and the herbicide-resistance gene bar from streptomyces hygroscopicus was used as the selection marker. transformants which showed a significant increase in chitosanase production (app ...200919093150
utility of molecular sequence analysis of the its rrna region for identification of fusarium spp. from ocular sources.fungal ocular infections cause significant ocular morbidity, particularly when diagnosis and treatment are delayed. accurate morphologic identification of fusarium spp. beyond the genus is time-consuming and insensitive. it was the purpose of this study to examine the usefulness of the nuclear ribosomal rna (rrna) internal transcribed spacer regions (its1 and -2) to detect and differentiate fusarium spp. responsible for ocular infections.200919136697
isolation and characterisation of polychlorinated biphenyl (pcb) degrading fungi from a historically contaminated soil.abstract:200919138404
metal based isatin-derived sulfonamides: their synthesis, characterization, coordination behavior and biological activity.some isatin derived sulfonamides and their transition metal [co(ii), cu(ii), ni(ii), zn(ii)] complexes have been synthesized and characterized. the structure of synthesized compounds and their nature of bonding have been inferred on the basis of their physical (magnetic susceptibility and conductivity measurements), analytical (elemental analyses) and spectral (ir, (1)h nmr and (13)c nmr) properties. an octahedral geometry has been suggested for co(ii), ni(ii) and zn(ii) and square-planar for cu ...200918825557
synthesis of organometallic-based biologically active compounds: in vitro antibacterial, antifungal and cytotoxic properties of some sulfonamide incorporated ferrocences.sulfonamides incorporated ferrocene (sif) have been synthesized by the condensation reaction of sulfonamides (sulfanilamide, sulfathiazole or sulfamethaxazole) with 1,1'-diacetylferrocene. the synthesized compounds (sif(1)-sif(4)) have been characterized by their physical, spectral and analytical properties and have been screened for their in vitro antibacterial properties against pathogenic bacterial strains e.g., escherichia coli, pseudomonas aeruginosa, bacillus subtilis staphylococcus aureus ...200918608785
in vitro antifungal and antibacterial activities of extracts of galium tricornutum subsp. longipedunculatum.the aim of this study was to evaluate the antimicrobial activity of crude ethanolic extracts and fractions of the ariel parts and the fruits of galium tricornutum subsp. longipedunculatum, traditionally used in northern areas of pakistan for treating microbial infections of skin. extracts and their fractions were tested against six bacteria and six fungal strains using the hole diffusion method and macrodilution method. all extracts and fractions possessed significant antimicrobial effect. four ...200918608788
[successful treatment of resistant fusarium solani keratitis with liposomal amphotericin b].the prognosis for fusarium keratitis is poor. effective drugs to treat this infection are therefore needed.200919942316
[characterization of a chitosanase from fusarium solani and its expression in an industrial strain of saccharomyces cerevisiae].to investigate the biochemical characteristics of the chitosanase from fusarium solani, and its application in chitooligosaccharides production, and express the chitosanase gene (csn) in a saccharomyces cerevisiae industrial strain.200920222446
onychomycosis caused by fusarium solani in a woman with diabetes.a case of untreated fusarial onychomycosis leading to serious consequences is reported. fusarium solani is a widespread fungus and an occasional human pathogen. it usually invades rapidly in immunocompromised hosts, and often results in a poor outcome despite treatment. we report a woman with diabetes mellitus who had untreated fusarial infection of the nails, which developed into subcutaneous fusariosis, superinfected by bacteria, and then evolved into osteomyelitis that subsequently resulted i ...200920055843
[culture-filtrate producing condition and biological activity of fusarium solani].to study the culture-filtrate producing condition of fusarium solani isolated from astragalus root and explore the mechanism astragalus root rot disease caused by, in order to find theoretical support for screening resistant germ plasma via mycotoxin.200920069894
enhancing the thermal stability of lipases through mutagenesis and immobilization on zeolites.the hydrolysis reaction of p-nitrophenyl butyrate catalyzed by lipases was followed with in situ uv/vis diode array spectrophotometry. five enzymes - candida antarctica lipase b and fusarium solani pisi cutinase wild-type and three single-mutation variants - were tested as catalysts in homogeneous conditions and immobilized on zeolite nay, on a polyacrylate support and as cross-linked aggregates. using deconvolution techniques and kinetic modeling, the thermal stability of the different biocatal ...200918443829
improvement of sec-dependent secretion of a heterologous model protein in bacillus subtilis by saturation mutagenesis of the n-domain of the amye signal peptide.due to the lack of an outer membrane, gram-positive bacteria (e.g., bacillus species) are considered as promising host organisms for the secretory production of biotechnologically relevant heterologous proteins. however, the yields of the desired target proteins were often reported to be disappointingly low. here, we used saturation mutagenesis of the positively charged n-domain (positions 2-7) of the signal peptide of the bacillus subtilis alpha-amylase (amye) as a novel approach for the improv ...201020077115
two cutinase-like proteins secreted by mycobacterium tuberculosis show very different lipolytic activities reflecting their physiological function.cutinases are extracellular enzymes that are able to degrade cutin, a polyester protecting plant leaves and many kinds of lipids. although cutinases are mainly found in phytopathogenic fungi or bacteria, 7 genes related to the cutinase family have been predicted in the genome of mycobacterium tuberculosis. these genes may encode proteins that are involved in the complex lipid metabolism of the bacterium. here, we report on the biochemical characterization of two secreted proteins of m. tuberculo ...201020103719
molecular phylogenetic diversity of dermatologic and other human pathogenic fusarial isolates from hospitals in northern and central italy.fifty-eight fusaria isolated from 50 italian patients between 2004 and 2007 were subject to multilocus dna sequence typing to characterize the spectrum of species and circulating sequence types (sts) associated with dermatological infections, especially onychomycoses and paronychia, and other fusarioses in northern and central italy. sequence typing revealed that the isolates were nearly evenly divided among the fusarium solani species complex (fssc; n = 18), the f. oxysporum species complex (fo ...201020107100
a case of primary localized cutaneous infection due to fusarium oxysporum.fusarium is a ubiquitous hyalohyphomycete isolated from food, widespread in the environment (plants, soil) and present at all latitudes. fusarium oxysporum and fusarium solani are the most frequent pathogenic species, followed by f. moniliforme and f. chlamydosporum. infections due to this mold may be disseminated or localized. localized forms include cutaneous and subcutaneous infection, onychomycosis, endophtalmitis, otitis, sinusitis, arthritis, osteomyelitis, and brain abscess. disseminated ...201020177971
endophytic fungi diversity of aquatic/riparian plants and their antifungal activity in vitro.two hundred and fourteen endophytic fungi were isolated from 500 segments of aquatic/riparian plants ottelia acuminata, myriophyllum verticillatum, equisetum arvense, cardamine multijuga, and impatiens chinensis. they were identified to 31 taxa in which cladosporium, fusarium, and geotrichum were the dominant genera. among all isolates, 169 (79%) were anamorphic fungi, 1 (0.5%) was an teleomorphic ascomycete and 44 (21%) were sterile mycelia. there were significant differences in the colonizatio ...201020221722
the microbiology of lascaux cave.lascaux cave (montignac, france) contains paintings from the upper paleolithic period. shortly after its discovery in 1940, the cave was seriously disturbed by major destructive interventions. in 1963, the cave was closed due to algal growth on the walls. in 2001, the ceiling, walls and sediments were colonized by the fungus fusarium solani. later, black stains, probably of fungal origin, appeared on the walls. biocide treatments, including quaternary ammonium derivatives, were extensively appli ...201020056706
multi-enzyme inhibition assay for the detection of insecticidal organophosphates and carbamates by high-performance thin-layer chromatography applied to determine enzyme inhibition factors and residues in juice and water samples.esterase inhibition assays provide an effect-directed tool of rapid screening for inhibitors in environmental and food samples. according to a multi-enzyme microtiter-plate assay, rabbit liver esterase (rle), bacillus subtilis esterase (bs2), and cutinase from fusarium solani pisi (cut) were used for the detection of 21 organophosphorus and carbamate pesticides by high-performance thin-layer chromatography-enzyme inhibition assays (hptlc-ei). staining was performed with fast blue salt b coupling ...201020060788
efficacy of posaconazole as treatment and prophylaxis against fusarium solani.invasive fusariosis is a highly aggressive fungal infection associated with high mortality in heavily immunocompromised patients. although posaconazole is efficacious as salvage therapy against infections caused by fusarium species, concerns remain regarding this agent in the setting of reduced potency. to evaluate the efficacy of posaconazole as treatment or prophylaxis against invasive fusariosis caused by fusarium solani, we utilized a neutropenic murine model of disseminated disease. icr mic ...201020065054
acidified nitrite enhances hydrogen peroxide disinfection of acanthamoeba, bacteria and fungi.in the human innate immune system, stimulated phagocytes release reactive nitrogen intermediates that can react with superoxide to form the powerful oxidant peroxynitrite and other less abundant species. in this study, the efficacy of hydrogen peroxide (h2o2) and acidified nitrite (nano2) alone and in combination was compared against a variety of bacteria, fungi and protozoa.201020335189
metal based biologically active compounds: design, synthesis, and antibacterial/antifungal/cytotoxic properties of triazole-derived schiff bases and their oxovanadium(iv) complexes.a new series of oxovanadium(iv) complexes have been designed and synthesized with a new class of triazole schiff bases derived from the reaction of 3,5-diamino-1,2,4-triazole with 2-hydroxy-1-naphthaldehyde, pyrrole-2-carboxaldehyde, pyridine-2-carboxaldehyde and acetyl pyridine-2-carboxaldehyde, respectively. physical (magnetic susceptibility, molar conductance), spectral (ir, (1)h nmr, (13)c nmr, mass and electronic) and analytical data have established the structures of these synthesized schi ...201020338672
[mycotic keratitis in an eye care hospital in mexico city].some of the most common precipitating events for keratomycoses (fungal keratitis), include surgical trauma (after cornea transplantation), the use of contaminated contact lenses or alterations in lacrimal secretions. diagnosis and treatment (to avoid loss of vision) for these type of infections are challenging.201020346302
other fungi causing onychomycosis.nondermatophyte onychomycosis account for 2% to 12% of all nail fungal infections and can be caused by a wide range of fungi, mainly scopulariopsis brevicaulis, aspergillus versicolor, a. flavus, a. niger, a. fumigatus, fusarium solani, f. oxysporum and scytalidium spp. among the predisposing factors are footwear, hyperhidrosis, local trauma, peripheral circulatory disease, and immunosuppression. these nondermatophyte fungi lack the keratinolytic capacity of dermatophytes, but they still can inf ...201020347658
mycotic keratitis in india: a five-year retrospective study.mycotic keratitis is a fungal infection of the cornea. this infection is difficult to treat and it can lead to severe visual impairment or blindness. it is worldwide in distribution, but is more common in the tropics and subtropical regions. trauma is the major predisposing factor, followed by ocular and systemic defects, prior application of corticosteroids, and prolonged use of antibiotic eye-drops. the objective of this study was to determine causative agents and to identify the predisposing ...201020351459
knock down of chitosanase expression in phytopathogenic fungus fusarium solani and its effect on pathogenicity.chitosanases are lytic enzymes involved in the degradation of chitosan, a component of fungal cell walls. the phytopathogenic fungus fusarium solani produces an extracellular chitosanase, csn1, the role of which in the physiology and virulence of the fungus remains to be expounded. here, we studied the expression of the csn1 gene through gene silencing and examined its effect on fungal pathogenicity. a vector construct encoding a hairpin rna (hprna) of csn1 was constructed and introduced into th ...201020419375
direct site-directed photocoupling of proteins onto surfaces coated with beta-cyclodextrins.a method called dock'n'flash was developed to offer site-specific capture and direct uva-induced photocoupling of recombinant proteins. the method involves the tagging of recombinant proteins with photoreactive p-benzoyl-l-phenylalanine (pbpa) by genetic engineering. the photoreactive pbpa tag is used for affinity capture of the recombinant protein by beta-cyclodextrin (beta-cd), which provides hydrogen atoms to be abstracted in the photocoupling process. to exemplify the method, a recombinant, ...201020441154
do multipurpose contact lens disinfecting solutions work effectively against non-fda/iso recommended strains of bacteria and fungi?recent outbreaks of microbial keratitis have increased concerns about the efficacy of multipurpose solutions (mps) against 'real-world' organisms. this study determined, in accordance with fda/iso standard methods, the effects of five mps against clinical isolates and type strains of bacteria, and isolates of fungi from subjects' ocular structures; and of three mps against environmental fungal isolates.201020444107
the genomic organization of plant pathogenicity in fusarium species.comparative genomics is a powerful tool to infer the molecular basis of fungal pathogenicity and its evolution by identifying differences in gene content and genomic organization between fungi with different hosts or modes of infection. through comparative analysis, pathogenicity-related chromosomes have been identified in fusarium oxysporum and fusarium solani that contain genes for host-specific virulence. lateral transfer of pathogenicity chromosomes, inferred from genomic data, now has been ...201020471307
miniemulsion as efficient system for enzymatic synthesis of acid alkyl esters.the aim of this work is to devise an efficient enzymatic process for the production of linear alkyl esters in aqueous miniemulsion systems. the esterification reactions of linear alcohols and carboxylic acids were performed with three different enzymes, commercial amano lipase ps from pseudomonas cepacia, lipase type vii from candida rugosa, and lyophilized fusarium solani pisi cutinase expressed in saccharomyces cerevisiae su50. the miniemulsion system shows a high potential for the synthesis o ...201020503297
fungal outbreak in a show cave.castañar de ibor cave (spain) was discovered in 1967 and declared a natural monument in 1997. in 2003 the cave was opened to public visits. despite of extensive control, on 26 august 2008 the cave walls and sediments appeared colonized by long, white fungal mycelia. this event was the result of an accidental input of detritus on the afternoon of 24 august 2008. we report here a fungal outbreak initiated by mucor circinelloides and fusarium solani and the methods used to control it.201020553941
natural occurrence of the fusarium solani on tityus stigmurus (thorell, 1876) (scorpiones: buthidae).members of the fusarium solani species complex are agents of human mycoses, also affecting plants and other animals. nevertheless, this fungus has not been reported on scorpions. ten specimens of tityus stigmurus collected in the field and showing their surface covered by white mycelia were used to assess fungus presence in the animal after its death. identification of the fungi was based upon the cultural and morphological characteristics. the fungus was isolated from chelicerae and intersegmen ...201020231972
synthesis and antileishmanial and antimicrobial activities of some 2,3-disubstituted 3h-quinazolin-4-ones.a series of 2,3-disubstituted 3h-quinazolin-4-ones was synthesized. antimicrobial activities of the synthesized compounds were investigated against gram (+ve) and gram (-ve) bacteria, including b. subtilis, s. aureus, s. flexneri, p. aeruginosa, and s. typhi, and six fungi, namely trichophyton longifusus, candida albicans, aspergillus flavus, microsporum canis, fusarium solani, and candida glabrata using the broth microdilution method. compounds 9, 11, and 12 showed significant activities agains ...201020235747
transesterification of oil mixtures catalyzed by microencapsulated cutinase in reversed micelles.recombinant cutinase from fusarium solani pisi was used to catalyze the transesterification reaction between a mixture of triglycerides (oils) and methanol in reversed micelles of bis(2-ethylhexyl) sodium sulfosuccinate (aot) in isooctane for the purposes of producing biodiesel. the use of a bi-phase lipase-catalyzed system brings advantages in terms of catalyst re-use and the control of water activity in the medium and around the enzyme micro-environment. small-scale batch studies were performe ...201019943181
a novel class of peptide pheromone precursors in ascomycetous fungi.recently, sexual development in the heterothallic ascomycete trichoderma reesei (anamorph of hypocrea jecorina) has been achieved and thus initiated attempts to elucidate regulation and determinants of this process. while the α-type pheromone of this fungus fits the consensus known from other fungi, the assumed a-type peptide pheromone precursor shows remarkably unusual characteristics: it comprises three copies of the motif (li)gc(ts)vm thus constituting a caax domain at the c-terminus and two ...201020735770
fusarium keratitis 3 weeks after healed corneal cross-linking.to report a case of fusarium solani keratitis after corneal cross-linking (cxl) treatment.201020795586
novel short antibacterial and antifungal peptides with low cytotoxicity: efficacy and action mechanisms.short antimicrobial peptides with nine and eleven residues were developed against several clinically important bacterial and fungal pathogens (specifically escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, candida albicans, and fusarium solani). twelve analogues of previously reported peptides bp76 (kklfkkilkfl) and pac-525 (kwrrwvrwi) were designed, synthesized, and tested for their antimicrobial activities. two of our eleven amino acid peptides, p11-5 (gklfkkilkil) and p11-6 (kk ...201020603106
development of a new mlst scheme for differentiation of fusarium solani species complex (fssc) isolates.fungi belonging to the fusarium solani species complex (fssc) are well known plant pathogens. in addition to being the causative agent of some superficial infections, fssc has recently emerged as a group of common opportunistic moulds, mainly in patients with haematological malignancies. molecular typing methods are essential in order to better understand the epidemiology of such opportunistic agents with the final goal of preventing contamination. a three-locus typing scheme has thus been devel ...201020624428
antimicrobial activity of simulated solar disinfection against bacterial, fungal, and protozoan pathogens and its enhancement by riboflavin.riboflavin significantly enhanced the efficacy of simulated solar disinfection (sodis) at 150 watts per square meter (w m(-2)) against a variety of microorganisms, including escherichia coli, fusarium solani, candida albicans, and acanthamoeba polyphaga trophozoites (>3 to 4 log(10) after 2 to 6 h; p < 0.001). with a. polyphaga cysts, the kill (3.5 log(10) after 6 h) was obtained only in the presence of riboflavin and 250 w m(-2) irradiance.201020639371
agar block smear preparation: a novel method of slide preparation for preservation of native fungal structures for microscopic examination and long-term storage.we describe a novel method of fungal slide preparation named "agar block smear preparation." a total of 510 agar block smears of 25 fungal strains obtained from culture collections, 90 qc fungal strains, and 82 clinical fungal strains from our clinical microbiology laboratory, which included a total of 137 species of yeasts, molds, and thermal dimorphic fungi, were prepared and examined. in contrast to adhesive tape preparation, agar block smears preserved the native fungal structures, such as i ...201020660221
biodegradation of anthracene and benz[a]anthracene by two fusarium solani strains isolated from mangrove sediments.an investigation was undertaken on the biodegradation of two kinds of polycyclic aromatic hydrocarbons (pahs), anthracene (ant) and benz[a]anthracene (baa), by fungi isolated from pah-contaminated mangrove sediments environment in ma wan, hong kong. ant (50mg l(-1)) and baa (20mg l(-1)), respectively, were added to mineral salt medium initially for screening of pah-degrading fungi, and finally two fungal species capable of using ant or baa as the sole carbon source were isolated and identified a ...201020691587
purification and characterization of an extracellular laccase from the anthracene-degrading fungus fusarium solani mas2.an extracellular laccase was purified from the culture medium of the non-white rot, anthracene-degrading fungal strain fusarium solani mas2. both native page and sds-page revealed one single band corresponding to a molecular weight of about 72 kda. treatment with endoglycosidase h reduced the molecular weight by 12%. the purified laccase maintained stable at ph 3-11 and up to 50 degrees c. the highest activity was detected at ph 3.0 and at 70 degrees c. the enzyme retained 46.2-97.2% of it activ ...201020716485
a polycationic antimicrobial and biocompatible hydrogel with microbe membrane suctioning ability.despite advanced sterilization and aseptic techniques, infections associated with medical implants have not been eradicated. most present coatings cannot simultaneously fulfil the requirements of antibacterial and antifungal activity as well as biocompatibility and reusability. here, we report an antimicrobial hydrogel based on dimethyldecylammonium chitosan (with high quaternization)-graft-poly(ethylene glycol) methacrylate (dmdc-q-g-em) and poly(ethylene glycol) diacrylate, which has excellent ...201021151166
changing indications for lamellar keratoplasty in shandong, 1993 - 2008.with the advancement of microsurgical techniques, lamellar keratoplasty (lk) has been more valued and performed to treat corneal blindness. this study aimed to evaluate the indications and changing trends for lk during the past 16 years in shandong eye institute, an eye center in china.201021163128
antifungal activity of pvd1 defensin involves plasma membrane permeabilization, inhibition of medium acidification, and induction of ros in fungi cells.in recent years, studies have demonstrated the function of many antimicrobial peptides against an extensive number of microorganisms that have been isolated from different plant species and that have been used as models for the study of various cellular processes linked to these peptides' activities. recently, a new defensin from phaseolus vulgaris (l.) seeds, named pvd(1,) was isolated and characterized. pvd(1) was purified through anion exchange and phase-reverse chromatography. pvd(1)'s antif ...201021170711
[fusarium solani infection in a patient after allogeneic hemotopoietic stem cell transplantation: case report and literature review].to study fusarium solani infection as a complication in patients after allogeneic hemotopoietic stem cell transplantation and to discuss the diagnosis and appropriate therapy.201021176501
oxidation of elemental sulfur by fusarium solani strain thif01 harboring endobacterium bradyrhizobium sp.nineteen fungal strains having an ability to oxidize elemental sulfur in mineral salts medium were isolated from deteriorated sandstones of angkor monuments. these fungi formed clearing zone on agar medium supplemented with powder sulfur due to the dissolution of sulfur. representative of the isolates, strain thif01, was identified as fusarium solani on the basis of morphological characteristics and phylogenetic analyses. pcr amplification targeting 16s rrna gene and analyses of full 16s rrna ge ...201020571793
pr-1, a novel antifungal protein from pumpkin rinds.a novel antifungal protein, m(r) = ca. 40 kda, was isolated from pumpkin rind and designated pr-1. when purified by anion exchange chromatography and hplc, it inhibited growth of several fungi including botrytis cinerea, fusarium oxysporum, fusarium solani and rhizoctonia solani, as well as the yeast, candida albicans, at 10-20 microm. it did not inhibit growth of escherichia coli or staphylococcus aureus even at 200 microm. laser scanning microscopy of fungal cells exposed to rhodamine-labeled ...201019760117
fusarium solani is responsible for mass mortalities in nests of loggerhead sea turtle, caretta caretta, in boavista, cape verde.the fungus fusarium solani (mart.) saccardo (1881) was found to be the cause of infections in the eggs of the sea turtle species caretta caretta in boavista island, cape verde. egg shells with early and severe symptoms of infection, as well as diseased embryos were sampled from infected nests. twenty-five isolates with similar morphological characteristics were obtained. their its rrna gene sequences were similar to the genbank sequences corresponding to f. solani and their maximum identity rang ...201020875054
direct enzymatic acylation of cellulose pretreated in bmimcl ionic liquid.cellulose esters are an important class of functional biopolymers with great interest in the chemical industry. in this work the enzymatic acylation of avicel cellulose with vinyl propionate, vinyl laurate and vinyl stearate, has been performed successfully in a solvent free reaction system. at first cellulose was putted into the ionic liquid bmimcl (1-n-butyl-3-methylimidazolium chloride) in order to facilitate the unwrap of the structure of the polysaccharide molecule and make it accessible to ...201020888759
successful treatment of fusarium solani ecthyma gangrenosum in a patient affected by leukocyte adhesion deficiency type 1 with granulocytes transfusions.ecthyma gangrenosum (eg) manifests as a skin lesion affecting patients suffering extreme neutropenia and is commonly associated with pseudomonas aeruginosa in immunocompromised patients. leukocyte adhesion deficiency i (lad i) which count among primary immunodeficiency syndromes of the innate immunity, is an autosomal recessive disorder characterized in its severe phenotype by a complete defect in cd18 expression on neutrophils, delayed cord separation, chronic skin ulcers mainly due to recurren ...201020929531
dermatitis in captive wyoming toads (bufo baxteri) associated with fusarium spp.from may 2007 to june 2008, 30 of 49 wyoming toads (bufo baxteri) kept at omaha's henry doorly zoo (nebraska, usa) died showing clinical signs of ventral erythema, inappetance, lethargy, and delayed righting reflex. treatment with antifungals and antibiotics was unsuccessful in all cases. histopathologic analyses revealed dermatitis as the primary problem in 20 of 21 toads in which skin was examined. fungal dermatitis was present in 17 toads, with hyphae approximately 1-3 μm in diameter, and par ...201020966269
transgenically expressed rice germin-like protein1 in tobacco causes hyper-accumulation of h2o2 and reinforcement of the cell wall components.our recent report documented that the rice germin-like protein1 (osglp1), being a cell wall-associated protein involves in disease resistance in rice and possesses superoxide dismutase (sod) activity as recognized by heterologous expression in tobacco. in the present study, the transgenic tobacco plants were analyzed further to decipher the detailed physiological and biochemical functions of the osglp1 and its associated sod activity. the transgenic tobacco lines expressing sod-active osglp1 sho ...201020971065
enzymes for the biofunctionalization of poly(ethylene terephthalate).the functionalization of synthetic polymers such as poly(ethylene terephthalate) to improve their hydrophilicity can be achieved biocatalytically using hydrolytic enzymes. a number of cutinases, lipases, and esterases active on polyethylene terephthalate have been identified and characterized. enzymes from fusarium solani, thermomyces insolens, t. lanuginosus, aspergillus oryzae, pseudomonas mendocina, and thermobifida fusca have been studied in detail. thermostable biocatalysts hydrolyzing poly ...201021076908
elimination of hydrophobic volatile organic compounds in fungal biofilters: reducing start-up time using different carbon sources.fungal biofilters have been recently studied as an alternative to the bacterial systems for the elimination of hydrophobic volatile organic compounds (voc). fungi foster reduced transport limitation of hydrophobic vocs due to their hydrophobic surface and extended gas exchange area associated to the hyphal growth. nevertheless, one of their principal drawbacks is their slow growth, which is critical in the start-up of fungal biofilters. this work compares the use of different carbon sources (gly ...201021077141
[expression of integrin during the course of adhesion between fungi and corneal epithelial cells].to investigate the expression of integrin during the course of adhesion between fungi and corneal epithelial cells.201021092564
identification of fusarium species in formalin-fixed and paraffin-embedded sections by in situ hybridization using peptide nucleic acid probes.fusarium has recently emerged as an opportunistic pathogen of humans, but the histological differentiation of fusarium from aspergillus and scedosporium is particularly difficult because these fungi may induce similar clinical features and exhibit filamentous development in host tissues. thus, there is a need to establish rapid and reliable methods that are applicable to pathological diagnoses. the aim of this study was to evaluate and establish in situ hybridization (ish) using peptide nucleic ...201021106796
[identification of pathogens causing root rot of sophora tonkinensis].to identify the pathogens what caused of root rot, it can provide method of theoretical gist of integrated pest management of these kinds of diseases in the future.201021355186
elimination of hydrophobic volatile organic compounds in fungal biofilters: reducing start-up time using different carbon sources.fungal biofilters have been recently studied as an alternative to the bacterial systems for the elimination of hydrophobic volatile organic compounds (voc). fungi foster reduced transport limitation of hydrophobic vocs due to their hydrophobic surface and extended gas exchange area associated to the hyphal growth. nevertheless, one of their principal drawbacks is their slow growth, which is critical in the start-up of fungal biofilters. this work compares the use of different carbon sources (gly ...201021404250
antifungal activity of rhizospheric bacteria.fluorescent pseudomonad spp. were isolated from the rhizosphere of potato plants (algeria) by serial dilutions of rhizosphere soils on kings b medium and were tested for their antifungal activity. the antifungal activity of the pseudomonas isolated from potatoes rhizosphere was tested against pythium ultimum, rhizoctonia solani and fusarium oxysporum in dual culture with bacteria on pda. the petri dish was divided into tow, on one the bacteria was spread and on the opposite side fungal plugs wer ...201021534477
fusarium solani: an emerging fungus in chronic diabetic ulcer.fusarium species, a mold which causes disease mainly in plants has emerged as pathogen in immunocompromised patients. fusarium is known to cause keratitis, onychomycosis, and endophthalmitis. fusarium solani, is the most common isolate from clinical specimen. here is a case, a 65-year-old male with type ii diabetes mellitus since 10 years presented with a large ulcer on the left leg since 8 months following trauma. the fungal culture of the escar of the ulcer isolated a mold, fusarium solani. th ...201021814405
development of a nested pcr assay for the detection of fusarium solani dna and its evaluation in the diagnosis of invasive fusariosis using an experimental mouse model.fusarium infections are increasingly being encountered in immunocompromised patients. fusarium solani accounts for nearly half of these infections. a specific nested pcr (npcr) assay has been developed by using dna isolated from several fusarium species and other common fungi. furthermore, dna samples isolated from bronchoalveolar lavage (bal) and serum samples from mice infected intravenously with f. solani conidia and sacrificed on every third day post infection were used for the evaluation of ...201019076280
a dark strain in the fusarium solani species complex isolated from primary subcutaneous sporotrichioid lesions associated with traumatic inoculation via a rose bush thorn.fusarium species are hyaline hyphomycetes widely distributed in nature and documented agents of both superficial and systemic infections in humans. in this paper, we report a darkly-pigmented and initially non-sporulating isolate in the fusarium solani species complex (fssc) causing a post-traumatic sporotrichoid infection in an otherwise healthy, male patient. sequencing of multiple loci showed that the isolate represented an otherwise unknown lineage, possibly corresponding to a separate speci ...201020055744
endophytic fungal strains of fusarium solani, from apodytes dimidiata e. mey. ex arn (icacinaceae) produce camptothecin, 10-hydroxycamptothecin and 9-methoxycamptothecin.camptothecin and 10-hydroxycamptothecin are two important precursors for the synthesis of the clinically useful anticancer drugs, topotecan and irinotecan. in recent years, efforts have been made to identify novel plant and endophytic fungal sources of camptothecin and 10-hydroxycamptothecin. in this study we have isolated endophytic fungi strains from apodytes dimidiata (icacinaceae), a medium sized tree from the western ghats, india. the fungi were identified as fusarium solani using both its ...201019863979
a murine model of contact lens-associated fusarium keratitis.fusarium solani and f. oxysporum were the causative organisms of the 2005/2006 outbreak of contact lens-associated fungal keratitis in the united states. the present study was an investigation of the ability of f. oxysporum grown as a biofilm on silicone hydrogel contact lenses to induce keratitis.201019875664
biodegradation of low-density polyethylene (ldpe) by isolated fungi in solid waste medium.in this study, biodegradation of low-density polyethylene (ldpe) by isolated landfill-source fungi was evaluated in a controlled solid waste medium. the fungi, including aspergillus fumigatus, aspergillus terreus and fusarium solani, were isolated from samples taken from an aerobic aged municipal landfill in tehran. these fungi could degrade ldpe via the formation of a biofilm in a submerged medium. in the sterilized solid waste medium, lpde films were buried for 100 days in a 1-l flask containi ...201019919893
pyrene degradation and copper and zinc uptake by fusarium solani and hypocrea lixii isolated from petrol station soil.this study aimed to isolate and identify potential polycyclic aromatic hydrocarbon (pah)-degrading and/or metal-tolerant fungi from pah-contaminated and metal-contaminated soils.201019922595
steady state and time resolved fluorescence quenching and chemical modification studies of a lectin from endophytic fungus fusarium solani.the solute quenching studies of a lectin from endophytic fungus fusarium solani were carried out using different quenchers such as acrylamide, succinimide, potassium iodide and cesium chloride. the lectin showed emission maximum at 348 nm indicating relative exposure of tryptophan. the quenchable fraction of the fluorophore was 100% with acrylamide, whereas it was only 50% with succinimide. the ionic quenchers iodide and cesium showed opposite effects at different ph. in the case of cesium, rais ...201019823920
endophytic bacteria from ocimum sanctum and their yield enhancing capabilities.endophytes are beneficial microbes that reside intercellularly inside the plants. interaction of endophytes with the host plants and their function within their host are important to address ecological relevance of endophyte. four endophytic bacteria os-9, os-10, os-11, and os-12 were isolated from healthy leaves of ocimum sanctum. these isolated microbes were screened in dual culture against various phytopathogenic fungi viz. rhizoctonia solani, sclerotium rolfsii, fusarium solani, alternaria s ...201019826860
design of eudragit rl 100 nanoparticles by nanoprecipitation method for ocular drug delivery.the objective of the current study was to prepare positively charged amphotericin-b-loaded nanoparticles providing a controlled release formulation. the particles were prepared by solvent displacement or nanoprecipitation method. the non-biodegradable positively charged polymer eudragit rl 100 was used to prepare the different formulations with varying ratios of drug and polymer. the formulations were evaluated in terms of particle size, zeta potential, and differential scanning calorimetry meas ...201019800990
invasive fusariosis with prolonged fungemia in a patient with acute lymphoblastic leukemia: case report and review of the literature.fusariumspp are rare but important opportunistic pathogens in immunocompromised patients. disseminated fusarial infections occur mostly in patients with hematologic malignancies with myelosuppressive chemotherapy or in patients with severe immunodeficiency. although more frequent than aspergillus fungemia, fusarium fungemia remains a rare event. we describe the case of a female patient with febrile neutropenia and persistent fungemia due to fusarium solani, treated with posaconazole and liposoma ...201019665409
fungal biomass is a key factor affecting polymorphonuclear leucocyte-induced hyphal damage of filamentous fungi.previous studies have not systematically assessed the effect of fungal biomass on polymorphonuclear leucocyte (pmn)-induced hyphal damage (hd) of filamentous fungi. we hypothesised that fungal biomass is a significant factor affecting pmn-induced hd. one isolate each consisting of a volume of 2 x 10(4) conidia ml(-1) of aspergillus fumigatus, aspergillus flavus, aspergillus terreus, rhizopus oryzae, rhizopus microsporus, cunninghamella bertholletiae, scedosporium prolificans and fusarium solani ...201019638003
quantitative determination of gibberellins by high performance liquid chromatography from various gibberellins producing fusarium strains.high performance liquid chromatographic (hplc) method was developed for analysis of seven gibberellins, i.e., ga3, ga4, ga7, ga3 methyl ester, ga7 methyl ester 3,13 diacetate, ga7 methyl ester, and fusaric acid, using an isocratic system. method was used for estimation of gibberellins from different fusarium strains. gibberellins were extracted from 28 strains of fusarium, out of which six strains of fusarium were isolated from soil of different parts of india and 22 strains were procured from t ...201019626451
backbone dynamics of the antifungal psd1 pea defensin and its correlation with membrane interaction by nmr spectroscopy.plant defensins are cysteine-rich cationic peptides, components of the innate immune system. the antifungal sensitivity of certain exemplars was correlated to the level of complex glycosphingolipids in the membrane of fungi strains. psd1 is a 46 amino acid residue defensin isolated from pea seeds which exhibit antifungal activity. its structure is characterized by the so-called cysteine-stabilized alpha/beta motif linked by three loops as determined by two-dimensional nmr. in the present work we ...201019632194
in vitro antagonism of cotton seedlings fungi and characterization of chitinase isozyme activities in trichoderma harzianum.the antagonistic fungus trichoderma harzianum is widely recognized as a potential biocontrol agent against several soil-borne plant pathogens. t. harzinum is rich source of chitinoltic enzymes. in vitro screening of 5 isolates of t. harzinum, one isolate of chaetomium globosum and one isolate of conetherium mentance, revealed that all of them had reduced growth area of macrophomina phaseolina, fusarium solani and rhizoctonia solani on pda medium, significantly. the inhibition percentage ranged f ...201023961072
a new furoquinoline alkaloid with antifungal activity from the leaves of ruta chalepensis l.bioassay-guided separation with an eye toward antifungal activity led to the isolation of the new alkaloid 5-(1̀,1̀-dimethylallyl)-8-hydroxyfuro[2-3-b] quinoline (1) and the known biscoumarin daphnoretin (2) as the active constituents of the chloroform extract obtained from the leaves of ruta chalepensis. the structures of the metabolites were elucidated on the basis of their spectral characteristics (nmr, uv, and ms) and were compared with the literature. the antifungal activity of the isolated ...201022491304
characterization of bacillus isolates of potato rhizosphere from andean soils of peru and their potential pgpr characteristics.bacillus spp. are well known rhizosphere residents of many crops and usually show plant growth promoting (pgp) activities that include biocontrol capacity against some phytopatogenic fungi. potato crops in the andean highlands of peru face many nutritional and phytophatogenic problems that have a significant impact on production. in this context is important to investigate the natural presence of these microorganisms in the potato rhizosphere and propose a selective screening to find promising p ...201024031569
[severe keratomycosis due to fusarium solani induced by a telluric foreign body: about a case in moroccan sahara].we report a case of severe keratitis due to fusarium solani in a young man in the sahara in morocco where the climate is arid. this patient reported had a grain of sand in his right eye for a week after a sandstorm. on admission he had a corneal abscess. despite rapid diagnosis and initiation of treatment with available antifungal drugs: amphotericin b and natamycin eye drops, the prognosis worsened and led to the enucleation of the right eye. faced with a suspected eye infection after a microtr ...201124451564
molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran.fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ...201123961146
diversity of microfungi in sandy beach soil of teluk aling, pulau pinang.a total of 82 isolates of microfungi were isolated from 6 sandy soil samples collected from teluk aling beach, pulau pinang. the soil microfungi were isolated by using direct isolation, debris isolation and soil dilution techniques. based on morphological characteristics, seven genera of microfungi were identified namely, fusarium (42%), aspergillus (24%), trichoderma (13%), curvularia (9%), colletotrichum (6%), helminthosporium (4%) and penicillium (2%). the most common species isolated was fus ...201124575210
[mycetomas diagnosed in senegal from 2008 to 2010].mycetomas are inflammatory pseudotumours of subcutaneous and possibly osseous soft fabrics, generally polyfistulas with chronic mode of evolution.201124451559
fusarium solani onychomycosis of the thumbnail coinfected with pseudomonas aeruginosa: report of two cases.fusarium species are non-dermatophytic moulds, which are commonly known soil saprophytes and important plant pathogens, and have been frequently reported to be aetiological agents of opportunistic infections in humans. the prevalence of onychomycosis caused by fusarium species varies in the literature because of geographical differences in mould distribution and diagnostic methods. onychomycosis caused by fusarium species is considered rare in korea, and only four cases have been described to da ...201119751392
antibacterial effects of enniatins j(1) and j(3) on pathogenic and lactic acid bacteria.enniatins (ens) are n-methylated cyclohexadepsipeptides, secondary metabolites produced by various species of the genus fusarium. they are known to act as antifungal, antiyeast and antibacterial and to possess antiinsecticidal and phytotoxic properties. in this study we evaluated for the first time the antibiotic effect of pure fractions of en j(1) and j(3) on several pathogenic strains and lactic acid bacteria. the ens j(1) and j(3) were purified from the fermentation extract of fusarium solani ...201121742008
in vitro antifungal activity of e1210, a novel antifungal, against clinically important yeasts and moulds.e1210 is a new antifungal compound with a novel mechanism of action and broad-spectrum of antifungal activity. we investigated the in vitro antifungal activity of e1210 compared to that of fluconazole, itraconazole, voriconazole, amphotericin b, and micafungin against clinical fungal isolates. e1210 showed potent activity against most candida spp. (mic(90) of ôëñ0.008 to 0.06 ++g/ml) except for candida krusei (mics of 2 to >32 ++g/ml). e1210 showed equally potent activity against fluconazole-res ...201121825291
identification and comparison of cutinases for synthetic polyester degradation.cutinases have been exploited for a broad range of reactions, from hydrolysis of soluble and insoluble esters to polymer synthesis. to further expand the biotechnological applications of cutinases for synthetic polyester degradation, we perform a comparative activity and stability analysis of five cutinases from alternaria brassicicola (abc), aspergillus fumigatus (afc), aspergillus oryzae (aoc), humicola insolens (hic), and the well-characterized fusarium solani (fsc). of the cutinases, hic dem ...201121713515
extracellular xylanase production by fusarium species in solid state fermentation.fusarium sp. has been shown to be a promising organism for enhanced production of xylanases. in the present study, xylanase production by 21 fusarium sp. isolates (8 fusarium culmorum, 4 fusarium solani, 6 fusarium verticillioides and 3 fusarium equiseti) was evaluated under solid state fermentation (ssf). the fungal isolate fusarium solani syrn7 was the best xylanase producer among the tested isolates. the effects of some agriculture wastes (like wheat straw, wheat bran, beet pulp and cotton se ...201122184927
Alpha2-macroglobulin from an Atlantic shrimp: biochemical characterization, sub-cellular localization and gene expression upon fungal challenge.In this study, we report on the isolation and characterization of an alpha2-macroglobulin (a2M) from the plasma of the pink shrimp Farfantepenaeus paulensis, its sub-cellular localization and transcriptional changes after infection by fungi. The molecular mass of the a2M was estimated at 389 kDa by gel filtration and 197 kDa by SDS-PAGE, under reducing conditions, suggesting that a2M from F. paulensis consists of two identical sub-units, covalently linked by disulphide bonds. The N-terminal amin ...201121888978
[Identification of soil-borne fungi using Fourier transform infrared spectroscopy].Fourier transform infrared (FTIR) attenuated total reflectance (ATR) spectroscopy was used in combination with multivariate statistic analysis for identification of soil-borne fungi that causes severe economic damage to agriculture: Fusarium monili forme, Fusarium semitectum, Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Sclerotinia sclerotiorum, Pythium aphanidermatum and Phytophthora capsici. The original FTIR spectra were normalized, and the second derivatives were calculated, from ...201122007392
the in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of marrubium vulgare l. essential oil grown in tunisia.in order to validate its antiseptic and anticancer properties with respect to traditional uses, we have screened for the first time the antimicrobial activity of aerial parts of m. vulgare l. essential oil against different pathogenic microorganisms and the cytotoxic activity against hela cell lines.201121936887
fungal endophthalmitis.fungal endophthalmitis (fe) is infrequent but results in poor visual outcomes. it can be exogenous or endogenous depending upon the mode of infection. the common causes for endogenous fe, post-traumatic fe and fe secondary to keratitis are candida albicans, aspergillus niger and fusarium solani, respectively. clinical features depend on the virulence of the organism and the mode of infection. broad-spectrum systemic antifungal therapy with or without intravitreal antifungal drugs is recommended. ...201122114969
performance of innovative pu-foam and natural fiber-based composites for the biofiltration of a mixture of volatile organic compounds by a fungal biofilm.the performance of perlite and two innovative carriers that consist of polyurethane (pu) chemically modified with starch; and polypropylene reinforced with agave fibers was evaluated in the biofiltration of a mixture of vocs composed of hexane, toluene and methyl-ethyl-ketone. at a total organic loading rate of 145gcm(-3)h(-1) the elimination capacities (ecs) obtained were 145, 24 and 96gcm(-3)h(-1) for the biofilters packed with the pu, the reinforced polypropylene, and perlite, respectively. s ...201122178276
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