Publications

dysbiosis of upper respiratory tract microbiota in elderly pneumonia patients.	bacterial pneumonia is a major cause of morbidity and mortality in elderly. we hypothesize that dysbiosis between regular residents of the upper respiratory tract (urt) microbiome, that is balance between commensals and potential pathogens, is involved in pathogen overgrowth and consequently disease. we compared oropharyngeal microbiota of elderly pneumonia patients (n=100) with healthy elderly (n=91) by 16s-rrna-based sequencing and verified our findings in young adult pneumonia patients (n=27) ...	2016	26151645
alterations in airway microbiota in patients with pao2/fio2 ratio ≤ 300 after burn and inhalation injury.	injury to the airways after smoke inhalation is a major mortality risk factor in victims of burn injuries, resulting in a 15-45% increase in patient deaths. damage to the airways by smoke may induce acute respiratory distress syndrome (ards), which is partly characterized by hypoxemia in the airways. while ards has been associated with bacterial infection, the impact of hypoxemia on airway microbiota is unknown. our objective was to identify differences in microbiota within the airways of burn p ...	2017	28358811
community analysis of dental plaque and endotracheal tube biofilms from mechanically ventilated patients.	mechanically ventilated patients are at risk for developing ventilator-associated pneumonia, and it has been reported that dental plaque provides a reservoir of respiratory pathogens that may aspirate to the lungs and endotracheal tube (ett) biofilms. for the first time, metataxonomics was used to simultaneously characterize the microbiome of dental plaque, etts, and non-directed bronchial lavages (nbls) in mechanically ventilated patients to determine similarities in respective microbial commun ...	2017	28259058
actinotignum schaalii subcutaneous abscesses in a patient with hidradenitis suppurativa: case report and literature review.	actinotignum schaalii (formerly actinobaculum schaalii) is a gram-positive, facultative anaerobic rod that is typically involved in urinary tract infections in elderly patients or those with underlying urological pathologies. in contrast, abscess formation caused by a. schaalii is very rare. we present a case of multiple abscesses in the perineal area in a young patient with hidradenitis suppurativa associated with a. schaalii and prevotella melaninogenica and review the relevant literature on t ...	2017	27913316
next-generation sequencing combined with specific pcr assays to determine the bacterial 16s rrna gene profiles of middle ear fluid collected from children with acute otitis media.	the aim of the study was to analyze the bacteriome of acute otitis media with a novel modification of next-generation sequencing techniques. outpatient children with acute otitis media were enrolled in the study, and middle ear fluids were collected during 90 episodes from 79 subjects aged 5 to 42 months (median age, 19 months). the bacteriome profiles of middle ear fluid samples were determined by a nested-pcr amplification of the 16s rrna gene (v4 region), followed by mass sequencing. the prof ...	2017	28357413
quantitative metagenomics reveals unique gut microbiome biomarkers in ankylosing spondylitis.	the assessment and characterization of the gut microbiome has become a focus of research in the area of human autoimmune diseases. ankylosing spondylitis is an inflammatory autoimmune disease and evidence showed that ankylosing spondylitis may be a microbiome-driven disease.	2017	28750650
black-pigmented anaerobic bacteria associated with ovine periodontitis.	periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. this study aimed to detect species of the genera porphyromonas and prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). the presence of microorganisms was evaluated by polymerase chain reaction (pcr) using specific primers for por ...	2017	28619155
influence of chronic azithromycin treatment on the composition of the oropharyngeal microbial community in patients with severe asthma.	this study of the oropharyngeal microbiome complements the previously published azithromycin in severe asthma (azisast) clinical trial, where the use of azithromycin was assessed in subjects with exacerbation-prone severe asthma. here, we determined the composition of the oropharyngeal microbial community by means of deep sequencing of the amplified 16s rrna gene in oropharyngeal swabs from patients with exacerbation-prone severe asthma, at baseline and during and after 6 months treatment with a ...	2017	28486933
oral microbial profiles of individuals with different levels of sugar intake.	the aim was to compare the oral microbial profiles in young adults with an intake of free sugars above or below the current recommendations by the who for sugar consumption. seventy subjects completed a quantitative food frequency questionnaire to establish the proportion of free sugars in relation to the total energy intake (% e). subjects with <5% e (n = 30) formed the low-sugar group, while those with ≥5% e (n = 40) were regarded as reference group. saliva and plaque samples were analyzed by ...	2017	28839520