Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| respiratory virus infection of peripheral blood monocytes: correlation with ageing of cells and interferon production in vitro. | the ability of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) to replicate in peripheral blood monocytes cultured in vitro for 1, 2, 4 or 7 days prior to infection was investigated. inoculation of 1-day old monocytes produced at least tenfold less new virus than infection of the older, more macrophage-like cells for both viruses. piv3 induced extensive syncytium formation, whereas rsv caused a cytopathic effect manifest by increased rounding of the cells with minimal syn ... | 1987 | 3035067 |
| effect of phorbol ester on growth of tumors induced by rous sarcoma virus and on pp60src kinase activity in these tumors. | the inoculation of rous sarcoma virus (rsv) into chickens results in the induction of tumors which usually grow progressively for several weeks and then regress. we have found that the direct injection of phorbol myristate acetate into growing rsv-induced sarcomas resulted in accelerated tumor regression in each of 11 cases studied. we further found that treatment of cultured rous sarcoma cells with phorbol myristate acetate for 72 h resulted in a 50% diminution in numbers of viable cells, in co ... | 1987 | 3036345 |
| haematological and pathomorphological studies on chickens inoculated with transformation-defective sarcoma virus. | a transformation-defective mutant of rous sarcoma virus (td pr-rsv-c) inoculated into chicken embryos caused a number of pathological changes in chickens after hatching. disturbances in haematopoiesis were manifested by anaemia of different degree and by the presence of immature blood elements in the peripheral blood. the haematological picture of erythroblastosis was confirmed histologically. in addition to erythroblastosis, lymphoid leukosis and a proliferative lymphosarcoma-like tumour were d ... | 1987 | 3036610 |
| unstable visible mutations induced in drosophila melanogaster by injections of oncogenic virus dna into the polar plasm of early embryos. | when rsv dna cloned in pbr 322 or dna of simian adenovirus sa7 (c8) is injected into the pole plasm of embryos of various drosophila stocks, the progeny of 1-70% of the surviving flies display visible mutations. the mutagenesis is partially directed: the loci mutating due to retrovirus and adenovirus dna do not overlap. the majority of resulting mutants are characterised by high instability: reversions and new mutations occur in them, which sometimes spread over the whole population ("explosive" ... | 1987 | 3037277 |
| expression of rous sarcoma virus transforming protein pp60v-src in saccharomyces cerevisiae cells. | the rous sarcoma virus (rsv) pp60v-src protein was expressed in saccharomyces cerevisiae cells either from a plasmid vector carrying the v-src gene or in yeast cells containing a single-copy v-src gene chromosomally integrated. in both yeast strains, v-src gene transcription is regulated by the galactose-inducible gal10 promoter. growth in galactose-containing medium resulted in constitutive expression of pp60v-src in the integrated strain and transient expression of higher levels of pp60v-src i ... | 1987 | 3037349 |
| mutants of the rous sarcoma virus envelope glycoprotein that lack the transmembrane anchor and cytoplasmic domains: analysis of intracellular transport and assembly into virions. | the envelope glycoprotein complex of rous sarcoma virus consists of a knoblike, receptor-binding gp85 polypeptide that is linked through disulfide bonds to a membrane-spanning gp37 spike. we used oligonucleotide-directed mutagenesis to assess the role of the hydrophobic transmembrane region and hydrophilic cytoplasmic domain of gp37 in intracellular transport and assembly into virions. early termination codons were introduced on either side of the hydrophobic transmembrane region, and the mutate ... | 1987 | 3041017 |
| noncoding region between the env and src genes of rous sarcoma virus influences splicing efficiency at the src gene 3' splice site. | viral rna and proteins in chicken embryo fibroblasts infected with different cloned variants of the prague strain rous sarcoma virus (rsv) were analyzed. the ratio of immunoprecipitated pp60src to the gag gene product p27 in prague a (pra) and prague b (prb) rsv-infected cells was two to three times that in prague c (prc) rsv-infected cells. a significant increase in the steady-state ratio of spliced 2.7-kilobase src gene mrna to unspliced 9.3-kilobase genome-size rna was observed in pra- and pr ... | 1987 | 3023695 |
| genetic alterations of integrated avian sarcoma virus dna sequences in transformed rat cells. | clones and subclones of schmidt-ruppin-rsv-d-(srd-) infected rat cells that were isolated in soft agar have shown differences in their morphology. some of the subclones were round or spindle-shaped cells with lower anchorage dependence and high growth rate, while others were more fibroblast-like and failed to overgrow one another. in correlation to their morphology, subclones with high degree of phenotypic transformation contained amplified amounts of full length proviral dna or proviral fragmen ... | 1987 | 3026480 |
| cytoskeletal association of pp60src. the transforming protein of the rous sarcoma virus. | immunoferritin labelling methods have been employed to examine the distribution of the rous sarcoma virus (rsv)-transforming protein pp60src in the detergent-resistant cytoskeleton of transformed cells. pp60src was found to be localized on actin microfilaments present in adhesion plaques, at adherens junctions between cells and also in microfilament bundles. this localization is consistent with the hypothesis that some of the morphological effects of transformation result from the interaction in ... | 1987 | 3026821 |
| comparison of the structural organizations in the 3'-terminal regions of five avian retrovirus strains: rav 7, rav 50, b77, pr-b, and sr-b. | in order to obtain information on the phylogenies of viral strains which belong to rsv (rous sarcoma virus) and alv (avian leukosis virus), the nucleotide sequences of noncoding regions adjacent to the u3 region in two alv strains, rous-associated virus 7 (rav 7) and rav 50, and three rsv strains, bratislava 77 (b77), prague:subgroup b (pr-b), and schmidt-ruppin:subgroup b (sr-b) were determined by extension from a common primer. the sequences thus deduced were compared with known sequences of o ... | 1987 | 3027964 |
| comparison of hep-2 cell culture and abbott respiratory syncytial virus enzyme immunoassay. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract infection in children. rapid identification of rsv infections would allow for specific chemotherapy. we evaluated a means of rapid diagnosis, the abbott enzyme immunoassay (eia), by using 314 stored nasopharyngeal aspirates. rsv antigens were identified in 62 of 66 rsv culture-positive specimens. an additional 37 specimens from which rsv was not isolated were positive in the eia. of these, 29 were confirmed as trul ... | 1987 | 3546371 |
| critical evaluation of viral antigen data in paget's disease of bone. | this study evaluates previous viral antigen data obtained from fixed tissue sections and cells grown in culture from bone affected by paget's disease. finding antigens to both respiratory syncytial virus (rsv) and measles virus (mv) in the same osteoclasts of ten patients could not be explained on the basis of any previously known cross-reactivity. therefore, possible causes for these observations were sought. monoclonal antibodies to viral proteins of rsv and mv were used to label proteins. pol ... | 1987 | 3549088 |
| immunoglobulin administration and ribavirin therapy: efficacy in respiratory syncytial virus infection of the cotton rat. | we studied the effects of combined administration of human immunoglobulin (ivig) and ribavirin aerosol on respiratory syncytial virus (rsv) infection in cotton rats (sigmodon hispidus). cotton rats assigned to receive combined therapy were administered gamimune, a preparation of purified ivig with a high titer of anti-rsv neutralizing activity, intraperitoneally 24 h prior to intranasal rsv challenge and then treated with ribavirin aerosol 3 days after challenge. lung viral titers from these cot ... | 1987 | 3550674 |
| effectiveness of topically administered neutralizing antibodies in experimental immunotherapy of respiratory syncytial virus infection in cotton rats. | initial studies of the prophylactic effect of parenterally administered respiratory syncytial virus (rsv)-neutralizing antibodies in cotton rats indicated that virus replication in lung tissues was restricted when animals with preexisting antibody titers in serum of 1:100 or more (as measured by plaque reduction) were challenged intranasally with 10(4) pfu of virus. subsequently, a therapeutic effect of parenterally administered rsv antibodies (present in human gamma globulin) was demonstrated i ... | 1987 | 3553614 |
| aerosolized ribavirin treatment of respiratory syncytial virus infection in infants hospitalized during an epidemic. | thirty-three infants with predisposing conditions and/or severely symptomatic with respiratory syncytial virus (rsv) infection were treated with aerosolized ribavirin during a 12-week period at oklahoma children's memorial hospital. these patients were compared with 97 untreated patients with rsv infection hospitalized during the same epidemic. despite preconditions which selected for a more seriously ill treatment group, patients who received ribavirin showed prompter resolution of the illness ... | 1987 | 3562136 |
| rous sarcoma virus-transformed fibroblasts and cells of monocytic origin display a peculiar dot-like organization of cytoskeletal proteins involved in microfilament-membrane interactions. | by immunofluorescence and interference reflection microscopy (irm) we found that f-actin and a group of cytoskeletal proteins involved in microfilament-membrane interaction, including vinculin, alpha-actinin, fimbrin and talin, are specifically organized in discrete dot-like structures corresponding to cell-substratum contact sites in both monocytes and monocyte-derived cells such as macrophages and osteoclasts. these proteins have a precise topological distribution; vinculin and talin form a do ... | 1987 | 3028844 |
| inhibition of methylation at two internal n6-methyladenosine sites caused by gac to gau mutations. | we previously have mapped n6-methyladenosine (m6a) sites within the genomic rna of rous sarcoma virus (rsv). the results of that study and of experiments using inhibitors of methylation suggest that m6a might be involved in mrna processing events. we describe an approach for directly analyzing the function of m6a in rna and for studying the sequence specificity of the m6a methylase. two sites of methylation in rsv (nucleotides 7414 and 7424) were altered by oligonucleotide-directed mutagenesis. ... | 1987 | 3029112 |
| comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay. | paired nasopharyngeal aspirate (npa) and nasopharyngeal swab (nps) specimens obtained from each of 32 hospitalized infants with x-ray-confirmed pneumonia (91%) or bronchiolitis were tested for respiratory syncytial virus (rsv) infection by virus culture, the indirect immunofluorescent-antibody (ifa) technique, enzyme-linked immunosorbent assay (elisa; ortho diagnostic systems, inc.), and spot hybridization with a human genomic probe to quantitate cellular dna. rsv was isolated in cell cultures f ... | 1987 | 3294883 |
| diagnostic efficacy of two rapid tests for detection of respiratory syncytial virus antigen. | with the availability of ribavirin therapy for serious respiratory syncytial virus (rsv) infections, rapid diagnostic tests for the detection of rsv antigen are increasingly important. efficacies of a commercially available enzyme immunoassay (eia) (abbott laboratories, north chicago, ill.) and a fluorescent-antibody assay (fa) were evaluated in a study involving 135 specimens from children with respiratory symptoms. a nasal wash specimen was cultured immediately on rsv-sensitive a549 cells; the ... | 1987 | 3294889 |
| [studies on the possible association between severe neurological syndromes and compulsory vaccination in milano]. | from june 1978 throughout february 1979, 66 children with a mean age of 13 months were admitted to a pediatric intensive care unit in naples (santobono hospital) after a rapid onset of coma or deep stupor of unknown ethiology. the "mystery disease" ("il male oscuro") as somebody in naples has defined this syndrome, was suspected to be associated with tetanus and diphtheria immunization. epidemiological investigations and an international symposium of experts agreed not to consider the cases due ... | 1987 | 3435655 |
| intravenous immunoglobulin treatment of respiratory syncytial virus infections in infants and young children. | respiratory syncytial virus (rsv)-infected cotton rats (sigmadon hispidus) and owl monkeys (aotus trivirgatus) showed significant reductions in rsv shedding from their respiratory tracts following parenteral therapy with human intravenous immunoglobulin (ivig) containing high titers of rsv-neutralizing antibody. because this therapy was well tolerated and appeared safe, a double-blind, placebo-controlled ivig immunotherapy pilot study was performed on 35 hospitalized, rsv-infected infants and ch ... | 1987 | 3439796 |
| antitumour effect of interferon combined with hyperthermia against experimental brain tumour. | antiproliferative activity of recombinant murine interferon-beta (rec-muifn-beta) combined with hyperthermia against rous sarcoma virus-induced mouse malignant glioma (rsv glioma) was investigated both in vitro and in vivo. in vitro, the antiproliferative activity of rec-muifn-beta was enhanced by incubation at elevated temperatures (40 degrees and 43 degrees c). in vivo, combined therapy of rec-muifn-beta treatment and local tumour hyperthermia (43 degrees c) exerted a greater antitumour effect ... | 1987 | 3480323 |
| instability of v-src sequences in nonhuman primate tumors cultured in vitro. | we have analyzed the dna of marmoset tumors induced and marmoset cells transformed by rous sarcoma virus (rsv) and derivative viruses of various types. southern blot hybridization was used to determine the presence of v-src gene sequences. we failed to detect v-src dna in high-passage cells derived from marmoset tumors induced in vivo or from marmoset cell lines transformed in vitro. the inability to detect src sequences was not related to selection of revertants in culture, since all cell lines ... | 1987 | 3111082 |
| association of marek's disease with ea-b and immune response genes in subline and f2 populations of the iowa state s1 leghorn line. | chickens from the iowa state s1 white leghorn line, selected for characteristics of ea-b serotype, humoral immune response to glutamic acid-alanine-tyrosine (ir-gat), and response to rous sarcoma virus (rsv)-induced tumors, were evaluated for genetic resistance to marek's disease (md). in the first two trials, sublines that were triple homozygous for the three traits were challenged with md virus. birds of the b1b1 blood type were significantly (p less than .001) more resistant to md than chicke ... | 1987 | 3112755 |
| expression and glycosylation of the respiratory syncytial virus g protein in saccharomyces cerevisiae. | a cdna encoding the entire amino acid sequence of the g glycoprotein of respiratory syncytial virus (rsv) was inserted into a yeast-escherichia coli shuttle vector such that expression of the virus gene was regulated by the yeast gal1 promoter. transformation of saccharomyces cerevisiae with the vector led to the formation of the g protein when cells were grown in the presence of galactose. under these conditions the rsv g appeared as a 60- to 65-kda glycosylated protein. expression of the g cdn ... | 1987 | 3113070 |
| binding of proteins, including pp60src, to activated ch-sepharose 4b. | activated ch-sepharose 4b and protein a sepharose cl-4b can bind, selectively and non-specifically, polypeptides from chick embryo cells. the major polypeptides bound have apparent molecular masses of 57-60 kda and 47-49 kda and cannot be eluted by extensive washing with buffers containing detergents. one of the 57-60 kda polypeptides was identified by immunoblotting as the transforming protein of rous sarcoma virus (rsv), pp60src. this polypeptide could be removed from the solid phase immunoabs ... | 1987 | 3118186 |
| effect of breast feeding on responses of systemic interferon and virus-specific lymphocyte transformation in infants with respiratory syncytial virus infection. | in order to elucidate the mechanisms of breast-feeding-induced resistance to respiratory syncytial virus (rsv) infection, groups of breast-fed and bottle-fed infants with this infection were tested at the onset of illness and then again 1 and 2 weeks later for the presence of interferon (ifn) as well as of virus-specific lymphocyte transformation (ltf) activity. alpha-ifn was detected in nine out of ten breast-fed infants, while it was found in only 11 out of 21 bottle-fed subjects. the rate of ... | 1987 | 3794673 |
| fatal pneumonia in an adult due to respiratory syncytial virus. | respiratory syncytial virus (rsv) infections in adults are generally mild, and no fatalities in uninstitutionalized adults have been reported. to our knowledge, we document herein the first case of fatal rsv pneumonia in a previously healthy elderly woman living at home, in whom complement fixation titers against rsv rose from less than 1:8 to reach 1:256 at death. cytoplasmic inclusions characteristic of rsv were detected at autopsy. | 1987 | 3827469 |
| expression of pp60v-src alters the ionic permeability of the plasma membrane in rat cells. | the transmembrane potential of rous sarcoma virus (rsv)-infected rat-1 cells, expressing the pp60v-src protein kinase, is markedly less negative (by approximately 30 mv) than that of their normal counterparts. by contrast, the membrane potential of rat-1 cells infected with kirsten sarcoma virus is virtually unaltered. the rsv-induced membrane depolarization is shown to be due to a severalfold increase in the cation permeability ratio (pna/pk) of the plasma membrane. when cells infected with a t ... | 1987 | 2434484 |
| detection of phosphotyrosine-containing proteins in polyomavirus middle tumor antigen-transformed cells after treatment with a phosphotyrosine phosphatase inhibitor. | cells transformed with the middle tumor antigen (mt) of polyomavirus were treated with sodium orthovanadate (na3vo4), an inhibitor of phosphotyrosine phosphatases, to enhance for the detection of cellular proteins which are phosphorylated on tyrosine. na3vo4 treatment of mt-transformed rat f1-11 cells resulted in a 16-fold elevation in the level of phosphotyrosine associated with total cellular proteins. parental f1-11 cells displayed only a twofold increase in phosphotyrosine following na3vo4 t ... | 1987 | 2434835 |
| interactions of retroviral structural proteins with single-stranded nucleic acids. | we have studied the interactions of single-stranded polyribonucleotides with murine leukemia virus structural proteins p10, p10' (a p10 variant), and pr65gag, as well as rous sarcoma virus (rsv) pp12 (a p10 analog). two quantitative assays have been used to monitor protein-rna association: the fluorescence enhancement of polyethenoadenylic acid) poly(epsilon a) upon binding protein, and tryptophan fluorescence quenching upon binding to poly(u). with each assay p10 was shown to bind stoichiometri ... | 1987 | 2435721 |
| recognition of rous sarcoma virus-induced tumor antigens by cytotoxic t lymphocytes (ctl): studies on specificity of killing by ctl employing h-2 congenic and recombinant mouse tumor cells. | the specificities of cytotoxic t lymphocytes (ctl) were studied for the analysis of ctl against tumor-specific cell surface antigen(s) (tssa) of non-virus-producing tumor cells induced by the schmidt-ruppin strain of rous sarcoma virus (sr-rsv) in b10 congenic and recombinant mice. eight ctl clones were established from immune spleen cells of b10.a(5r) mice. these clones demonstrated six patterns of cytotoxic reactivity in vitro: two clones showed h-2 restriction in tumor cell lysis. two other c ... | 1987 | 2436809 |
| induction of the ceruloplasmin mrna in balb/c 3t3 cells after rous-sarcoma virus transfection. | ceruloplasmin is a copper containing serum glycoprotein. it is an acute-phase protein and its levels are increased in inflammation and in a number of experimental and human tumors. it is normally synthesized in the liver and not in fibroblasts. in this paper we present evidence that ceruloplasmin mrna is synthesized in balb/c 3t3 cells and its levels are increased about 3-fold in rous-sarcoma virus (rsv) transformed cells. the ceruloplasmin mrnas from rat liver and rsv 3t3 cells have the same el ... | 1987 | 2438032 |
| altered pp60src kinase activity in regressing tumors induced by rous sarcoma virus. | tumors which are induced in chickens by rous sarcoma virus (rsv) usually grow progressively for several weeks and then regress. pp60src kinase activity was found to be reduced in cultured tumor cells which derived from regressing sarcomas by 60-75% as compared to progressively growing neoplasms. we further found that a cellular pp89 which co-precipitated with pp60src was largely diminished in tumor cells derived from regressing as compared with progressively growing sarcomas. in contrast, immuno ... | 1987 | 2438033 |
| histochemical staining of clonal mammalian cell lines expressing e. coli beta galactosidase indicates heterogeneous expression of the bacterial gene. | an evaluation has been made of the e. coli beta-galactosidase (beta-gal) gene for use as a reporter gene in mammalian cells in culture. we have adopted a histochemical procedure which enables identification of those cells within a population that express the introduced bacterial gene. data is presented concerning the sensitivity of the histochemical method relative to an immunological method of detection. it has been found that several clonal cell lines generated after transfection of human 293 ... | 1987 | 2440117 |
| modulation of monocyte mo2 surface antigen expression by exposure to respiratory syncytial virus. | the mo2 antibody recognizes a monocyte-specific cell surface antigen of unknown function. upward modulation of mo2 surface epitope density was demonstrated in response to 72-hr culture of monocytes with respiratory syncytial virus (rsv), but this was not seen after culture with phytohemagglutinin or other respiratory viruses. monoclonal antibody probes for rsv proteins were used to demonstrate probable replication of rsv proteins in peripheral blood monocytes and pulmonary alveolar macrophages. ... | 1987 | 2440300 |
| monoclonal antibodies to the large glycoproteins of respiratory syncytial virus: possible evidence for several functional antigenic sites. | employing different routes of immunization and different adjuvants, a large number of hybridomas were produced that secreted monoclonal antibodies (mabs) against the large envelope glycoprotein (gp90) and the fusion glycoprotein (gp70) of respiratory syncytial virus (rsv). the antigenic specificity of the mabs was established by immunoblot analysis and radioimmunoprecipitation. three hybridomas secreting anti-gp90 mabs were established from mice immunized by intraperitoneal injection of purified ... | 1987 | 2440982 |
| the g glycoprotein of human respiratory syncytial viruses of subgroups a and b: extensive sequence divergence between antigenically related proteins. | two major antigenic subgroups (designated a and b) have been described for human respiratory syncytial virus (rsv). previously, on the basis of reactivity patterns with monoclonal antibodies, the greatest intersubgroup variation was shown to occur in the g protein, the putative attachment glycoprotein. to delineate the molecular basis for this variation, we have determined the nucleotide and deduced amino acid sequences of the g mrnas and proteins representing a subgroup a (long strain) and a su ... | 1987 | 2441388 |
| antiviral chemotherapy and prophylaxis of viral respiratory disease. | respiratory viruses continue to be major causes of morbidity and mortality. currently available chemotherapy is limited to oral amantadine for uncomplicated influenza a and aerosolized ribavirin for respiratory syncytial virus (rsv) infections. amantadine is also efficacious for chemoprophylaxis of influenza a virus infections. rimantadine has similar clinical efficacy and is better tolerated than amantadine. aerosolized ribavirin may be useful in the treatment of serious respiratory illness cau ... | 1987 | 2446820 |
| feather pulp organ cultures for assessing host resistance to infection with avian leukosis-sarcoma viruses. | the purpose of this study was to improve in vitro procedures for detecting cellular resistance to the avian leukosis-sarcoma group of viruses. four feather pulp organ cultures (fpoc) were prepared from each chicken by placing pulp squeezed from feathers in wells of microtitre plates that contained culture medium. two of the four fpoc were inoculated with rous sarcoma virus (rsv) of subgroup a and 5 to 6 days later the fluids from all four cultures were assayed for virus by inoculating chicken em ... | 1987 | 18766632 |
| hatchability and mortality following infection of chick embryos with subgroup a rous sarcoma virus. | an investigation, using rhode island red, new hampshire, columbian and australorp, was conducted to study the correlation between chorioallantoic membrane (cam)-infection phenotype to challenge with bryan strain of rous sarcoma virus (bs-rsv) of subgroup a and subsequent mortality, following hatching. a total of 806 cams and 438 chicks over nine hatch-replicates in three experiments provided evidence that infection via an inoculation of the cam induced rapid and high mortality, about 72%, averag ... | 1988 | 18766714 |
| characterization of ribosomal frameshifting in hiv-1 gag-pol expression. | based on precedents from other retroviruses, the precursor of the human immunodeficiency virus (hiv-1) reverse transcriptase is predicted to be a polyprotein with a relative molecular mass (mr) of 160,000 (160k) encoded by both the viral pol gene and the upstream gag gene. these two genes lie in different translational reading frames, with the 3' end of gag overlapping the 5' end of pol by 205 or 241 nucleotides. thus, production of the gag-pol fusion protein would require either messenger rna p ... | 1988 | 2447506 |
| nonmyristoylated p60v-src fails to phosphorylate proteins of 115-120 kda in chicken embryo fibroblasts. | we have used anti-phosphotyrosine antibodies to identify a large number of tyrosine phosphoproteins in rous sarcoma virus (rsv)-transformed chicken embryo fibroblasts. most of these proteins were found in the 100,000 x g supernatant when cells were separated into soluble and particulate fractions; however, one group of proteins, of 115-120 kda, was found in the particulate fraction. the phosphotyrosine content of the 115- to 120-kda proteins was greatly reduced in chicken embryo fibroblasts infe ... | 1988 | 2451829 |
| identification of multiple novel polypeptide substrates of the v-src, v-yes, v-fps, v-ros, and v-erb-b oncogenic tyrosine protein kinases utilizing antisera against phosphotyrosine. | antibodies to phosphotyrosine were used to identify proteins phosphorylated on tyrosine in chicken embryo fibroblasts transformed by the oncogenes, v-src, v-yes, v-fps, v-ros, or v-erb-b. these antibodies allowed detection of a minimum of 50, 44, and 47 bands in immunoblots of cellular lysates from fibroblasts transformed by v-src, v-yes, or v-fps respectively. eight of these bands were also detected in fibroblasts transformed by v-ros and v-erb-b, suggesting that the cellular transformation ind ... | 1988 | 2452398 |
| separation of the tumor rejection antigen of rous sarcoma virus-induced murine fibrosarcoma. | the tumor antigen capable of inducing tumor resistance (tumor rejection antigen; tra) was separated and some of its physicochemical properties were characterized. cytosol and plasma membrane fractions were separated from rous sarcoma virus (rsv)-induced csa1m tumor cells. immunization with membrane but not cytosol fraction of these tumor cells together with complete freund's adjuvant resulted in complete protection against subsequent challenge with viable csa1m cells. the tra activity contained ... | 1988 | 2453498 |
| use of an epstein-barr virus episomal replicon for anti-sense rna-mediated gene inhibition in a human cytotoxic t-cell clone. | a methodology was developed for stable gene transfer into cloned nontransformed human t lymphocytes. stable high-level gene expression was achieved in cloned human t cells by using a self-replicating epstein-barr virus (ebv) episomal replicon. a comparison of five eukaryotic promoters established that the rous sarcoma virus 3' long terminal repeat (rsv 3' ltr) and the lymphopapilloma virus (lpv) 5' ltr are optimal for episome-based expression in t cells. effective (greater than 95%), selective, ... | 1988 | 2453884 |
| enforced expression of the c-myc oncogene inhibits cell differentiation by precluding entry into a distinct predifferentiation state in g0/g1. | a broad base of data has implicated a role for the c-myc proto-oncogene in the control of the cell cycle and cell differentiation. to further define the role of myc in these processes, i examined the effect of enforced myc expression on several events that are thought to be important steps leading to the terminally differentiated state: (i) the ability to arrest growth in g0/g1, (ii) the ability to replicate the genome upon initiation of the differentiation program, and (iii) the ability to lose ... | 1988 | 2454393 |
| an enzyme-linked immunosorbent assay using monoclonal antibodies for the detection of respiratory syncytial virus in clinical specimens. | an enzyme-linked immunosorbent assay (elisa) has been developed for the detection of respiratory syncytial virus in nasopharyngeal secretions. this assay employed as immunoreagents two monoclonal antibodies directed against two distinct epitopes of the viral nucleocapsid. one of them (rsv 4) was used for antigen capture and the other (nc 4) was labelled with n-hydroxy-succinimide-epsilon-caproil biotin and used for antigen detection. streptavidin biotin-peroxidase complexes were employed as ampl ... | 1988 | 2455493 |
| immunological characterization of proteins detected by phosphotyrosine antibodies in cells transformed by rous sarcoma virus. | phosphotyrosine antibodies were used to identify tyrosine-phosphorylated proteins in rous sarcoma virus (rsv)-transformed chicken embryo fibroblasts. a large number of tyrosine phosphoproteins were detected. a similar set of proteins was observed in rsv-transformed murine cells. an 85,000-dalton protein, however, was present in transformed avian cells but missing in transformed murine cells. neither the 85,000-dalton protein nor any of the other tyrosine phosphoproteins appeared to be viral stru ... | 1988 | 2455815 |
| vanadate-treated baby hamster kidney fibroblasts show cytoskeleton and adhesion patterns similar to their rous sarcoma virus-transformed counterparts. | rous sarcoma virus-transformed baby hamster kidney fibroblasts (rsv/b4-bhk) adhere to a fibronectin-coated substratum by means of dot-like adhesion sites called podosomes in view of their shape and function as cellular feet (tarone et al.: exp cell res 159:141, 1985). podosomes concentrate tyrosine-phosphorylated proteins, including pp60v-src, and appear in many cells transformed by oncogenes coding for tyrosine kinases. in this paper we used orthovanadate, an inhibitor of phosphotyrosine phosph ... | 1988 | 2456294 |
| tonsillitis and respiratory infections of viral aetiology in a developing country. | among 75 clinically diagnosed cases of tonsillitis, 55 (73.3%) were found to be of viral aetiology. serological and virological investigations identified adenovirus as the main causative agent in children as well as in adults. in only two instances, both in children, was an association with respiratory syncytial virus (rsv) in tonsillitis detected, an extremely rare finding. a cross-sectional sero-epidemiological study of respiratory infections caused by adenovirus, rsv, influenza (a,b) and para ... | 1988 | 2456726 |
| rapid titration of bovine, caprine and human rs virus by a micro-immunoperoxidase assay using a monoclonal antibody and a permissive ovine kidney cell line. | an indirect immunoperoxidase micro-assay, using a continuous cell line derived from ovine kidney cells (ok) and a previously characterized monoclonal antibody (7c2), specific for an exposed and highly conserved epitope of the fusion protein of different strains of rs virus, was used advantageously to rapidly titrate bovine, caprine and human strains of rsv by either quantal (tcid50) or plaque forming assays. virus titers, obtained in less than 36 h, were in agreement with those obtained by the c ... | 1988 | 2458375 |
| an immunoperoxidase method of detecting respiratory syncytial virus antigens in paraffin sections of pneumonic bovine lung. | using an avidin-biotin-peroxidase complex immunoperoxidase staining method, respiratory syncytial virus (rsv) antigen was demonstrated in glutaraldehyde-fixed, paraffin-processed lung sections from calves with induced rsv pneumonia. the virus also was detected in formalin-fixed, paraffin-processed lung sections from calves with naturally occurring rsv pneumonia. specific immunoperoxidase staining was detected within the cytoplasm of epithelial cells and syncytia in small bronchi, bronchioli, and ... | 1988 | 2458691 |
| characteristics of in vitro production of mucosal antibody to respiratory syncytial virus in tonsillar tissue lymphocytes. | tonsillar lymphocytes (tl) obtained from 39 subjects were established in culture in vitro, and analyzed for respiratory syncytial virus (rsv) specific antibody synthesis after stimulation with pokeweed mitogen and live or ultraviolet light inactivated rsv. following stimulation with the virus, igg1, iga, and low levels of igg3 but no igg2, igg4, or ige anti-rsv antibody could be detected in the culture supernatants. the anti-rsv antibody production in tl did not show any correlation with the pre ... | 1988 | 2458875 |
| small finger protein of avian and murine retroviruses has nucleic acid annealing activity and positions the replication primer trna onto genomic rna. | retrovirus virions carry a diploid genome associated with a large number of small viral finger protein molecules which are required for encapsidation. our present results show that finger protein p12 of rous sarcoma virus (rsv) and p10 of murine leukaemia virus (mulv) positions replication primer trna on the replication initiation site (pbs) at the 5' end of the rna genome. an rsv mutant with a val-pro insertion in the finger motif of p12 is able to partially encapsidate genomic rna but is not i ... | 1988 | 2458920 |
| formalin-inactivated respiratory syncytial virus vaccine induces antibodies to the fusion glycoprotein that are deficient in fusion-inhibiting activity. | the fusion (f) glycoprotein of respiratory syncytial virus (rsv) induces neutralizing antibodies and antibodies that inhibit fusion of infected cells (fi antibody). it was previously shown that infants and children immunized with formalin-inactivated rsv 20 years ago developed antibodies that bound to the f glycoprotein but were deficient in neutralizing activity. a reexamination of these sera indicated that they were also deficient in fi activity. thus, formalin-inactivated rsv vaccine stimulat ... | 1988 | 2459154 |
| proposed gag-encoded transcriptional activator is not necessary for rous sarcoma virus replication or transformation. | it has been reported that gene expression directed by the long terminal repeat of rous sarcoma virus (rsv) is trans activated by a protein encoded in an alternate reading frame within the rsv gag gene (s. broome and w. gilbert, cell 40:537-546, 1985). we have made specific mutations to test the role of the putative transcriptional activator in rsv replication. termination codons were created within the alternate reading frame coding for the trans activator, and the mutations were introduced into ... | 1988 | 2459410 |
| neutralization of respiratory syncytial virus by individual and mixtures of f and g protein monoclonal antibodies. | we identified several types of neutralization effected by f and g protein monoclonal antibodies (mabs) reacted individually or as mixtures against respiratory syncytial virus (rsv). neutralizing activity was identified by a microneutralization test in which virus replication was determined by enzyme immunoassay. complete neutralization was seen only with mabs against the f protein. strain-specific neutralization, complete neutralization against some strains of rsv, and no neutralization against ... | 1988 | 2459412 |
| transduction of proto-src sequences in tissue culture by a molecular clone of transformation-defective rous sarcoma virus with an internal src deletion. | the sporadic appearance of nondefective (nd) rous sarcoma virus (rsv) from cells in tissue culture infected with a molecular clone of transformation-defective rsv was examined. southern analysis of extrachromosomal, virus-specific dna of three independent ndrsv isolates in each case indicated restoration of an isogenic src by homologous recombination with cellular proto-src. the frequency of transduction was estimated by fluctuation analysis to vary between one transduction per 0.4 x 10(7) to 1. ... | 1988 | 2460643 |
| a selectable temperature-sensitive v-src moloney retrovirus. | we have cloned the v-src gene of ts339b77 rsv into a new moloney-based retroviral expression vector (yn). in the resulting construct (ts339ynsrc), the ts339 gene is transcribed from the viral ltr, while a selectable resitance marker, the tn5 neomycin phosphotransferase (neor) gene, is transcribed from an internal thymidine kinase (tk) promoter. g418 resistance and focus formation were induced in nih3t3 cells at comparable efficiencies within the permissive temperature range (33-37 degrees c). at ... | 1988 | 2485257 |
| commercial monoclonal antibodies for rapid detection of respiratory syncytial virus by direct immunofluorescence. | a commercially available direct immunofluorescence (if) test using a reagent consisting of a pool of two monoclonal antibodies against selected surface and internal proteins of respiratory syncytial virus (rsv) was evaluated in comparison with the indirect if technique using a commercial bovine anti-rsv hyperimmune serum for the rapid detection of rsv in nasopharyngeal aspirates from 228 hospitalized children. overall agreement between the two if methods was 95%. the direct if test was quicker t ... | 1988 | 3134216 |
| [isolation and characteristics of antibodies against synthetic fragments of oncoproteins. ii. antiserum against the transforming protein pp60src of rous sarcoma virus]. | to generate the antibodies to the transforming protein of rous sarcoma virus (rsv) pp60src, rabbits were immunized with the peptide, corresponding to 415-421 sequence of pp60src. these antibodies immunoprecipitate pp60src in rsv-transformed chicken and mammalian cells, and also some proteins (45, 85 and 120 kda), which could be autophosphorylated in vitro. it was shown that 415-421 sequence of pp60src is not recognized by the antibodies to pp60src from rsv-induced tumour bearing rabbits (tbr ser ... | 1988 | 3141779 |
| topically administered immunoglobulin reduces pulmonary respiratory syncytial virus shedding in owl monkeys. | a human intravenous immunoglobulin preparation containing neutralizing antibodies to respiratory syncytial virus (rsv) was utilized for intratracheal and topical treatment of laboratory-induced rsv infections in owl monkeys (aotus trivirgatus). topically administered antibodies induced significant reductions in rsv shedding in the lower respiratory tract. | 1988 | 3142351 |
| virus dilution affects the anti-rous sarcoma response of progressor but not regressor major histocompatibility (b) complex genotypes. | responses to tumors induced by three dilutions of rous sarcoma virus (rsv) were studied in unh 105, a noninbred line of new hampshire chickens. single-male matings produced progeny of three b genotypes: b23/b23 (tumor regressor) and b23/b24 and b24/b24 (tumor progressors). a total of 223 6-wk-old chicks were wingweb inoculated with one of three dilutions (10(-3), 4 x 10(-3), and 10(-4)) of a pseudotype of bryan high titer rsv designated as bh rsv (rav-1). a tumor profile index (tpi), an indicato ... | 1988 | 2853337 |
| helper t cell recognition of respiratory syncytial virus in mice. | in this study we aimed to define the protein and viral subtype specificities of helper th cells to respiratory syncytial virus (rsv). balb/c mice were primed by infection with rsv, or with vaccinia viruses (vv) containing genes encoding several individual rsv proteins. priming for th cell memory was assayed by stimulating spleen cells in vitro with different rsv isolates and measuring rsv-specific interleukin 2 (il-2) release by t cells into supernatants using an il-2-dependent ctll cell line. s ... | 1988 | 2963089 |
| respiratory syncytial virus infections and intravenous gamma-globulins. | the respiratory syncytial virus (rsv) is a common cause of bronchiolitis and pneumonia in infants and young children. throughout the world annual rsv epidemics result in numerous hospitalizations, substantial morbidity and some mortality. until the recent introduction of ribavirin only supportive therapy has been available for treating these infections. the development of animal models of rsv infection and the observation that some lots of immunoglobulin prepared for intravenous administration c ... | 1988 | 3041354 |
| respiratory syncytial virus: heterogeneity of subgroup b strains. | in order to investigate further possible structural differences among the two subgroups of respiratory syncytial virus (rsv), we analysed the antigenic characteristics and size of structural proteins of 20 subgroup a and 43 subgroup b strains by their reactions with monoclonal antibodies (mabs) directed against the proteins of rsv using immunofluorescence, elisa and radioimmunoprecipitation assays. the latter test also enabled determination of the size of different structural components. the 37 ... | 1988 | 3045252 |
| passive transfer of respiratory syncytial virus (rsv) antiserum suppresses the immune response to the rsv fusion (f) and large (g) glycoproteins expressed by recombinant vaccinia viruses. | in young infants who possess maternally derived respiratory syncytial virus (rsv) antibodies, the antibody response to rsv glycoproteins is relatively poor, despite extensive replication of rsv. in the present study, it was found that cotton rat rsv hyperimmune antiserum suppressed the antibody response to the rsv glycoproteins but not the response to vaccinia virus antigens when the antiserum was passively transferred to cotton rats prior to infection with vaccinia recombinant viruses expressin ... | 1988 | 3047432 |
| comparison of the abbott and ortho enzyme immunoassays and cell culture for the detection of respiratory syncytial virus in nasopharyngeal specimens. | a comparison of the abbott laboratories and the ortho diagnostic systems respiratory syncytial virus (rsv) enzyme immunoassays (eia) and hep-2 cell culture for the detection of rsv in 81 nasopharyngeal (np) specimens from pediatric patients with lower respiratory tract infection was carried out. the sensitivity and specificity of the abbott test compared to confirmed infection was 92.3% and 100.0%, respectively. the sensitivity and specificity of the ortho test was 87.5% and 80.3%, respectively. ... | 1988 | 3053011 |
| detection, pathogenesis, and therapy of respiratory syncytial virus infections. | respiratory syncytial virus (rsv) infection is a major cause of serious lower respiratory disease in infancy and early childhood. the unique pathogenesis of lower respiratory illness due to rsv offers some intriguing clues to the role of the human immune system in both protection against and development of respiratory illness. more than any other virus, rapid diagnostic techniques have been especially successful in identifying rsv infection. many of these techniques could be easily adaptable to ... | 1988 | 3060243 |
| respiratory syncytial virus. | rsv is now understood to be the most significant viral respiratory pathogen of infants and is capable of causing both bronchiolitis and pneumonia. it is a particular risk to hospitalized infants as the virus is easily spread through close contact. the most vulnerable infants are those who suffer with either congenital heart disease or bronchopulmonary dysplasia who easily fall prey to pulmonary complications of infection. strict environmental control and the use of protective clothing and eyewea ... | 1988 | 3066824 |
| time-resolved fluoroimmunoassay compared with virus isolation for rapid detection of respiratory syncytial virus in nasopharyngeal aspirates. | two monoclonal antibodies against two distinct conserved epitopes of the respiratory syncytial virus (rsv) nucleocapsid protein were used in a direct time-resolved fluoroimmunoassay (tr-fia) for the detection of rsv antigens in nasopharyngeal aspirates. the capture antibody was adsorbed to the solid phase of microdilution strip wells, and the indicator antibody was labeled with a europium chelate. specimens and label were incubated simultaneously for 1 h at 37 degrees c in the coated wells. afte ... | 1988 | 3068251 |
| [bronchiolitis caused by respiratory syncytial virus in very young infants and transmitted maternal antibodies]. | the study with an enzyme linked immunosorbent assay of the sera of 57 infants aged less than 9 weeks and developing a bronchiolitis, argues for the absence of a protective role for the maternally transmitted anti-respiratory syncytial virus (rsv) igg and for the existence of a positive relationship between these antibodies levels - which are not neutralizing - and the severity of rsv bronchiolitis. | 1988 | 3071286 |
| evaluation in non-human primates of the safety, immunogenicity and efficacy of recombinant vaccinia viruses expressing the f or g glycoprotein of respiratory syncytial virus. | it has been shown previously that immunization with recombinant vaccinia viruses expressing the f or g envelope glycoprotein of human respiratory syncytial virus (rsv) strain a2 induced a protective immune response in the lower respiratory tract of cotton rats against live rsv challenge. as a continuation of these studies, the safety, immunogenicity and efficacy of these recombinant vaccinia viruses was evaluated in non-human primates. rhesus and patas monkeys were each inoculated intradermally ... | 1988 | 3072795 |
| [comparison between immunofluorescence and immunoenzymatic assay for the rapid diagnosis of the respiratory syncytial virus in nasopharyngeal secretions]. | an enzyme immunoassay, rsv-eia abbot, was evaluated by comparison with indirect immunofluorescence. nasopharyngeal secretions obtained from 95 infants and young children with acute respiratory infections were examined for the presence of respiratory syncytial virus antigens with both methods. specimens were stored at -70 degrees c before being tested by eia. out of 60 samples positive by indirect immunofluorescence, 46 were also positive by rsv-eia (sensitivity 78.7%) and 34 out of 35 immunofluo ... | 1988 | 3073406 |
| multiple forms of growth inhibitors secreted from cultured rat liver cells: purification and characterization. | it was found that a non-tumorigenic epithelial cell line from the liver of a buffalo-strain rat (brl) secreted into the culture medium various inhibitors of the growth of brl and rsv-brl (tumorigenic brl transformed by infection of rous sarcoma virus). the secreted inhibitors were classified into two types: one inhibited the growth of brl to a greater extent than that of rsv-brl (non-tumorigenic brl growth inhibitor, ngi), and the other, vice versa (tumorigenic brl growth inhibitor, tgi). two ng ... | 1988 | 3170513 |
| the release of leukotrienes in the respiratory tract during infection with respiratory syncytial virus: role in obstructive airway disease. | samples of nasopharyngeal secretions from a group of 73 infants with bronchiolitis or upper respiratory illness alone during infection with respiratory syncytial virus were analyzed for leukotriene c4 (ltc4) content using a reverse-phase high-pressure liquid chromatography assay with confirmation by radioimmunoassay. titers of respiratory syncytial virus (rsv)-specific ige in nasopharyngeal secretion (nps) specimens were determined using an enzyme-linked immunosorbent assay. the highest concentr ... | 1988 | 3174295 |
| fluorescence and electron microscopic studies of the cytoskeletal organization of normal, established and transformed chick embryo cells. | the cytoskeletons of two established chick embryo cell (cec) lines were examined by fluorescence and electron microscopy and compared with those of control cells and cells transformed by rous sarcoma virus (rsv). in normal cec, many stress fibers were observed. on the other hand, stress fibers were disorganized in nontransformed spontaneously established cec, non-tumorigenic cec partially transformed with a chemical carcinogen, and tumorigenic rsv-transformed cec. in the normal cec, actin filame ... | 1988 | 3177004 |
| studies on the pathogenesis and interspecies transmission of respiratory syncytial virus isolated from sheep. | inoculation of lambs with an ovine isolate of respiratory syncytial virus (rsv) by a combined intranasal and intratracheal route resulted in mild respiratory tract illness, with respiratory tract lesions. lung lesions were characterized by bronchitis and bronchiolitis, hyperplasia of bronchial and bronchiolar epithelium, peribronchiolar and perivascular accumulations of lymphocytes, alveolar septal thickening, and collapse. respiratory syncytial virus was recovered from the respiratory tract of ... | 1988 | 3178037 |
| primary respiratory syncytial virus infection in mice. | a mouse model of respiratory syncytial virus (rsv) infection is described. a high-titered, large-volume inoculum results in replication of rsv to a high titer in lungs of balb/c mice. mice older than 15 weeks of age are more susceptible to rsv infection. titers up to 10(6.9) plaque-forming units (pfu)/gram lung can be attained in 32-week-old mice. older mice experience a clinical illness manifested by ruffled fur, reduced activity, and weight loss. lung histology of older mice infected with rsv ... | 1988 | 3183639 |
| role of respiratory syncytial virus in early hospitalizations for respiratory distress of young infants with cystic fibrosis. | to determine the frequency of respiratory syncytial virus (rsv) as the cause of hospitalization for acute pulmonary exacerbations in young infants with cystic fibrosis (cf), and to assess the clinical effects of rsv infections, we prospectively followed 48 children with a diagnosis of cf after identification by newborn screening. at a mean follow-up age of 28.8 months (range 5 to 59), 18 infants (38%) had been hospitalized a total of 30 times for acute respiratory distress. at the time of admiss ... | 1988 | 3183835 |
| fatal respiratory syncytial virus pneumonitis in a previously healthy child. | respiratory syncytial virus (rsv) is a cause of significant morbidity and mortality in infants and children with an immunocompromised status or a congenital heart disease. the following case describes a 6 8/12-year-old, previously normal child who had a fatal interstitial pneumonitis caused by rsv. documentation of rsv as the etiologic agent and documentation of her immune status are presented. in light of recent advances in the rapid diagnosis and treatment of rsv, this virus should be consider ... | 1988 | 3195838 |
| viruses and airborne allergens as precipitants of obstructive respiratory difficulties in children. | findings of virus infection were prospectively studied in 133 episodes of obstructive respiratory difficulty in 90 children aged 2 to 15 years. a viral infection was confirmed in 16 episodes (12%). in a further 11% of cases serological evidence of a recent viral infection was found. respiratory syncytial virus (rsv) accounted for half of the positive viral results. skin prick tests were performed after the second episode of bronchial obstruction. of the 79 children tested, 57% had at least one p ... | 1988 | 3218914 |
| [antigenic variants of the respiratory syncytial virus in argentina and uruguay]. | the characterization of rsv antigenic variants was carried out on 160 nasopharyngeal aspirates from children under 5 years of age, from argentina and uruguay, with acute respiratory infection and a previously positive etiological diagnosis for rsv. results for argentina were: 20.9% of samples belonged to subtype a, 76.9% to subtype b and the remaining 2.2% to new subtypes as yet uncharacterized. results for uruguay were: 5.6% of samples belonged to subtype a, 81% to subtype b and 13.5% to other ... | 1988 | 3231713 |
| [proliferative activity of normal, transformed and tumor cells in in vitro contact with syrian hamster neutrophils]. | the effect of casein-induced peritoneal neutrophils (nph) of syrian hamsters on the proliferative activity of normal, embryonal, transformed, and tumour cells was determined by their ability to inhibit or to stimulate 3h-thymidine incorporation in the replicating target cells. the enhanced proliferation of normal embryonal cells (he) of hamsters was observed after their in vitro contact with nph, in contrast to the cells of sfhe (spontaneously transformed in vitro he cells of low tumourigenicity ... | 1988 | 3243194 |
| [analysis of respiratory syncytial virus diseases in hospitalized children in the district of steyr 1984/85]. | respiratory syncytial virus (rsv) is the most frequent cause of serious respiratory tract infections in infancy. in the course of a one-year study the nasopharyngeal secretions of all hospitalized children with diseases of the lower respiratory tract (almost exclusively infants) were examined for viral antigens. rsv antigen could be detected in 34 of the 71 secretions. in these infants a serious clinical course, pneumonia, bronchiolitis or obstructive bronchitis was dominant, but laboratory test ... | 1988 | 3260367 |
| cytotoxic t cells clear virus but augment lung pathology in mice infected with respiratory syncytial virus. | we have examined the function of class i mhc-restricted cytotoxic t cells in experimental respiratory syncytial virus (rsv) infection of balb/c mice by transfer of t cell line mjc-a2 and ctl clone e8a into rsv-infected mice. the t cell line cleared pulmonary rsv infection within 5 d in persistently infected gamma-irradiated mice, but caused acute respiratory disease. this was only seen in infected mice and was often lethal after transfer of greater than 3 x 10(6) ctl. lower numbers of ctl produc ... | 1988 | 3262705 |
| seasonal variation in respiratory syncytial virus infections in children in benin-city, nigeria. | the seasonal variation in respiratory syncytial virus (rsv) infections in children was examined in benin city. nasopharyngeal washes were obtained from children under 3 years hospitalised for acute lower respiratory infections during two seasons - rainy season (june-august) and dry season (december-february). rsv surface antigen was identified by elisa. 54% of patients in the rainy season were elisa positive for rsv compared to 8.8% during the dry season. clinical features in the rsv infected pa ... | 1988 | 3265812 |
| detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay. | we evaluated prospectively the detection of respiratory syncytial virus (rsv) by culture and by direct antigen detection using an indirect immunofluorescence assay (ifa), a direct monoclonal immunofluorescence assay (dfa), and a monoclonal enzyme immunoassay (eia). of 221 specimens, 95 (43%) were culture positive for rsv, 4 (1.8%) contained more than one virus, and 17 (7.6%) contained a virus other than rsv. overall, hep-2 and flow 6000 cells grew significantly more rsv isolates (82 and 72%, res ... | 1988 | 3281981 |
| detection of an immunoglobulin m response in the elderly for early diagnosis of respiratory syncytial virus infection. | the indirect fluorescent-antibody technique was compared with indirect and mu-capture enzyme-linked immunosorbent assays for the detection of respiratory syncytial virus (rsv) immunoglobulin m (igm) in the elderly. sera from 47 patients (mean age, 70 years) with acute lower respiratory tract infections caused by rsv were investigated. specific igm was detected in 81% (38 of 47) of the patients. the fluorescent-antibody technique, which gave 70% positive results, proved to be the most sensitive o ... | 1988 | 3290237 |
| detection of respiratory syncytial virus antigen in nasopharyngeal secretions by abbott diagnostics enzyme immunoassay. | we compared a rapid respiratory syncytial virus (rsv) antigen enzyme immunoassay (eia) (abbott diagnostics, north chicago, ill.) with virus culture and with the indirect fluorescent-antibody test (fat) by using nasopharyngeal washings from children with suspected rsv pneumonia or bronchiolitis. fresh washings were used in all three tests. specimens were inoculated into hep-2 cells and human embryonic lung fibroblasts and observed for cytopathic effect. cells in the centrifuged sediments of the n ... | 1988 | 3290243 |
| occurrence of respiratory syncytial virus subgroup a and b strains in japan, 1980 to 1987. | the subgroup characteristics of 71 strains of respiratory syncytial virus (rsv) isolated in sapporo, japan, during 5 epidemic years from 1980 to 1987 were determined by the use of 17 monoclonal antibodies (mabs) raised against the rsv long strain, which is now recognized as the prototype subgroup a strain. nine of these mabs immunoprecipitated the fusion protein (f), five immunoprecipitated the large glycoprotein (g), two immunoprecipitated the nucleoprotein (np), and one immunoprecipitated the ... | 1988 | 3290246 |
| evaluation of two commercially available test methods to determine the feasibility of testing for respiratory syncytial virus in a community hospital laboratory. | the feasibility of performing respiratory syncytial virus (rsv) testing in a community hospital laboratory was assessed. the abbott rsv eia kit, an enzyme immunoassay (eia), and the ortho respiratory syncytial virus identification reagent, a direct fluorescent antibody (dfa) technique, were evaluated in terms of technologist time, result time, cost, and agreement of results. there was little difference in the amount of technologist time required for either method. the dfa technique was shown to ... | 1988 | 3293421 |
| comparison of cell culture and three enzyme-linked immunosorbent assays for the rapid diagnosis of respiratory syncytial virus from nasopharyngeal aspirate and tracheal secretion specimens. | a total of 211 specimens, including 144 nasopharyngeal aspirates and 67 tracheal secretions, were evaluated for the rapid detection of respiratory syncytial virus (rsv) antigen by three commercial enzyme-linked immunosorbent assays (elisa; kallestad diagnostic, austin, tx; ortho diagnostics, raritan, nj, and abbott laboratories, north chicago, il) and by isolation of rsv in cell culture. of the 61 rsv culture positive specimens, kallestad elisa, ortho elisa, and abbott elisa detected rsv antigen ... | 1988 | 3293894 |
| calcium requirement for syncytium formation in hep-2 cells by respiratory syncytial virus. | respiratory syncytial virus (rsv) grown in hep-2 cells in the absence of calcium did not induce cell fusion and syncytium formation. although the infected cells contained viral antigens, the cytopathic effect (giant cell formation) typical for rsv was not observed in calcium-free cultures. infectious virus yield was also slightly reduced (less than a one log10 reduction) in the absence of calcium. an analysis of viral proteins synthesized in both the presence and the absence of calcium revealed ... | 1988 | 3343306 |
| strain-specific serum antibody responses in infants undergoing primary infection with respiratory syncytial virus. | we sought to characterize the human respiratory syncytial virus (rsv) subgroup-specific antibody response to primary infection with rsv. rsv isolates from 43 infants and young children were typed as either subgroup a (long strain-like) or b (18537 strain-like) based on reactivity with monoclonal antibodies. acute-phase or preinfection and convalescent-phase sera were collected from the 43 subjects and tested in an enzyme-linked immunosorbent assay using purified f and g glycoproteins from rsv su ... | 1988 | 3346563 |
| prevention of nosocomial transmission of respiratory syncytial virus in a newborn nursery. | during three winter seasons prior to 1984-1985 the special care nursery at new england medical center experienced respiratory syncytial virus (rsv) epidemics that required closure of the unit. prior to and during the 1984-1985 winter season, several measures were taken to prevent recurrent nosocomial rsv transmission. in the winters of 1984-1985 and 1985-1986 there were 26 introductions of community-acquired rsv with no transmission of nosocomial cases during 1,688 patient days at risk as compar ... | 1988 | 3351266 |
| respiratory syncytial virus infection: a cause of respiratory distress syndrome and pneumonia in adults. | respiratory syncytial virus (rsv) infection, an important and sometimes lethal disease of infants and children, generally causes a milder and self-limited syndrome of cough, nasal congestion and fever in adults. while some evidence suggests that rsv may be responsible for more serious respiratory illness in the elderly and chronically ill, it has not been shown to cause life-threatening respiratory tract disease in previously healthy adults. this report describes a previously healthy woman who e ... | 1988 | 3354594 |
| modulation of systemic and mucosal immune responses to inhaled ragweed antigen in experimentally induced infection with respiratory syncytial virus implication in virally induced allergy. | groups of balb/c mice were either sham-infected or infected intranasally with respiratory syncytial virus (rsv). on the third day following intranasal inoculation, all groups of mice were exposed by inhalation to ragweed antigen for 5 consecutive days and rechallenged with ragweed on day 31. development of antibody activity to ragweed antigen was examined in serum and bronchial washings at regular intervals employing an elisa assay for igg and iga antibody activity and passive cutaneous anaphyla ... | 1988 | 3372039 |
| response of vaccinated and unvaccinated bighorn sheep (ovis canadensis canadensis) to experimental respiratory syncytial virus challenge. | five rocky mountain bighorn sheep (ovis canadensis canadensis), approximately 5 mo old and without detectable antibody titers to respiratory syncytial virus (rsv), were assigned to two groups to study the effects of rsv challenge inoculation in vaccinated (n = 3) and unvaccinated (n = 2) bighorns. the three lambs vaccinated with a modified live bovine rsv vaccine developed a detectable antibody response to the vaccine. vaccinated and unvaccinated lambs challenged with an ovine isolate of rsv dev ... | 1988 | 3373644 |