Publications

genetic diversity, reassortment, and recombination in alfalfa mosaic virus population in spain.	the variability and genetic structure of alfalfa mosaic virus (amv) in spain was evaluated through the molecular characterization of 60 isolates collected from different hosts and different geographic areas. analysis of nucleotide sequences in four coding regions--p1, p2, movement protein (mp), and coat protein (cp)--revealed a low genetic diversity and different restrictions to variation operating on each coding region. phylogenetic analysis of spanish isolates along with previously reported am ...	2014	24779352
plant-based vaccines: novel and low-cost possible route for mediterranean innovative vaccination strategies.	a plant bioreactor has enormous capability as a system that supports many biological activities, that is, production of plant bodies, virus-like particles (vlps), and vaccines. foreign gene expression is an efficient mechanism for getting protein vaccines against different human viral and nonviral diseases. plants make it easy to deal with safe, inexpensive, and provide trouble-free storage. the broad spectrum of safe gene promoters is being used to avoid risk assessments. engineered virus-based ...	2014	24751193
patellins 3 and 6, two members of the plant patellin family, interact with the movement protein of alfalfa mosaic virus and interfere with viral movement.	movement proteins (mps) encoded by plant viruses interact with host proteins to facilitate or interfere with intra- and/or intercellular viral movement. using yeast two-hybrid and bimolecular fluorescence complementation assays, we herein present in vivo evidence for the interaction between alfalfa mosaic virus (amv) mp and arabidopsis patellin 3 (atpatl3) and patellin 6 (atpatl6), two proteins containing a sec14 domain. proteins with sec14 domains are implicated in membrane trafficking, cytoske ...	2014	24751128
the movement protein (nsm) of tomato spotted wilt virus is the avirulence determinant in the tomato sw-5 gene-based resistance.	the avirulence determinant triggering the resistance conferred by the tomato gene sw-5 against tomato spotted wilt virus (tswv) is still unresolved. sequence comparison showed two substitutions (c118y and t120n) in the movement protein nsm present only in tswv resistance-breaking (rb) isolates. in this work, transient expression of nsm of three tswv isolates [rb1 (t120n), rb2 (c118y) and non-resistance-breaking (nrb)] in nicotiana benthamiana expressing sw-5 showed a hypersensitive response (hr) ...	2014	24690181
complete nucleotide sequence of alfalfa mosaic virus isolated from alfalfa (medicago sativa l.) in argentina.	the complete nucleotide sequence of an alfalfa mosaic virus (amv) isolate infecting alfalfa (medicago sativa l.) in argentina, amv-arg, was determined. the virus genome has the typical organization described for amv, and comprises 3,643, 2,593, and 2,038 nucleotides for rna1, 2 and 3, respectively. the whole genome sequence and each encoding region were compared with those of other four isolates that have been completely sequenced from china, italy, spain and usa. the nucleotide identity percent ...	2014	24510307
phosphorylation of alfalfa mosaic virus movement protein in vivo.	the 32-kda movement protein, p3, of alfalfa mosaic virus (amv) is essential for cell-to-cell spread of the virus in plants. p3 shares many properties with other virus movement proteins (mps); however, it is not known if p3 is posttranslationally modified by phosphorylation, which is important for the function of other mps. when expressed in nicotiana tabacum, p3 accumulated primarily in the cell walls of older leaves or in the cytosol of younger leaves. when expressed in pischia pastoris, p3 acc ...	2014	24435161
viral and chloroplastic signals essential for initiation and efficiency of translation in agrobacterium tumefaciens.	the construction of high-level protein expression vectors using the camv 35s promoter in concert with highly efficient translation initiation signals for agrobacterium tumefaciens is a relatively less explored field compared to that of escherichia coli. in the current study, we experimentally investigated the capacity of the camv 35s promoter to direct gfp gene expression in a. tumefaciens in the context of different viral and chloroplastic translation initiation signals. gfp expression and conc ...	2014	25117444
control of virus diseases in soybeans.	soybean, one of the world's most important sources of animal feed and vegetable oil, can be infected by numerous viruses. however, only a small number of the viruses that can potentially infect soybean are considered as major economic problems to soybean production. therefore, we consider management options available to control diseases caused by eight viruses that cause, or have the potential to cause, significant economic loss to producers. we summarize management tactics in use and suggest di ...	2014	25410106
structural studies on tobacco streak virus coat protein: insights into the pleomorphic nature of ilarviruses.	tobacco streak virus (tsv), the type member of ilarvirus genus, is a major plant pathogen. tsv purified from infected plants consists of a ss-rna genome encapsidated in spheroidal particles with diameters of 27, 30 and 33nm constructed from multiple copies of a single species of coat protein (cp) subunits. apart from protecting the viral genome, cps of ilarviruses play several key roles in the life cycle of these viruses. unlike the related bromo and cucumoviruses, ilarvirus particles are labile ...	2016	26706030
engineering and expression of a rhoa peptide against respiratory syncytial virus infection in plants.	main conclusion : a rhoa-derived peptide fused to carrier molecules from plants showed enhanced biological activity of in vitro assays against respiratory syncytial virus compared to the rhoa peptide alone or the synthetic rhoa peptide. a rhoa-derived peptide has been reported for over a decade as a potential inhibitor of respiratory syncytial virus (rsv) infection both in vitro and in vivo and is anticipated to be a promising alternative to monoclonal antibody-based therapy against rsv infectio ...	2016	26474991
the functional analysis of distinct tospovirus movement proteins (nsm) reveals different capabilities in tubule formation, cell-to-cell and systemic virus movement among the tospovirus species.	the lack of infectious tospovirus clones to address reverse genetic experiments has compromised the functional analysis of viral proteins. in the present study we have performed a functional analysis of the movement proteins (nsm) of four tospovirus species bean necrotic mosaic virus (benmv), chrysanthemum stem necrosis virus (csnv), tomato chlorotic spot virus (tcsv) and tomato spotted wilt virus (tswv), which differ biologically and molecularly, by using the alfalfa mosaic virus (amv) model sy ...	2017	27697453
the coat protein of alfalfa mosaic virus interacts and interferes with the transcriptional activity of the bhlh transcription factor ilr3 promoting salicylic acid-dependent defence signalling response.	during virus infection, specific viral component-host factor interaction elicits the transcriptional reprogramming of diverse cellular pathways. alfalfa mosaic virus (amv) can establish a compatible interaction in tobacco and arabidopsis hosts. we show that the coat protein (cp) of amv interacts directly with transcription factor (tf) ilr3 of both species. ilr3 is a basic helix-loop-helix (bhlh) family member of tfs, previously proposed to participate in diverse metabolic pathways. ilr3 has been ...	2017	26929142
detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in saudi arabia.	a total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in saudi arabia. das-elisa test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in saudi arabia. eighty percent of the alfalfa sampl ...	2017	28855829
within-host evolution of segments ratio for the tripartite genome of alfalfa mosaic virus.	the existence of multipartite viruses is an intriguing mystery in evolutionary virology. several hypotheses suggest benefits that should outweigh the costs of a reduced transmission efficiency and of segregation of coadapted genes associated with encapsidating each segment into a different particle. advantages range from increasing genome size despite high mutation rates, faster replication, more efficient selection resulting from reassortment during mixed infections, better regulation of gene e ...	2017	28694514
seed tolerance to deterioration in arabidopsis is affected by virus infection.	seed longevity is the period during which the plant seed is able to germinate. this property is strongly influenced by environment conditions experienced by seeds during their formation and storage. in the present study we have analyzed how the biotic stress derived from the infection of cauliflower mosaic virus (camv), turnip mosaic virus (tumv), cucumber mosaic virus (cmv) and alfalfa mosaic virus (amv) affects seed tolerance to deterioration measuring germination rates after an accelerated ag ...	2017	28477474