Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| snapshot and ce-sscp: two simple and cost-effective methods to reveal genetic variability within a virus species. | the multiplex snapshot and the capillary electrophoresis-single-strand conformation polymorphism (ce-sscp) procedures are here used for rapid and high-throughput description of the molecular variability of viral populations. both approaches are based on (1) standard amplification of genomic sequence(s), (2) labeled primers or labeled single-stranded dna, and (3) migration of fluorescent-labeled molecules in capillary electrophoresis system. the snapshot technology was used to describe the divers ... | 2015 | 25981256 |
| respiratory viruses in airline travellers with influenza symptoms: results of an airport screening study. | there is very little known about the prevalence and distribution of respiratory viruses, other than influenza, in international air travellers and whether symptom screening would aid in the prediction of which travellers are more likely to be infected with specific respiratory viruses. | 2015 | 25959149 |
| enveloped virus flocculation and removal in osmolyte solutions. | our ability to reduce infectious disease burden throughout the world has been greatly improved by the creation of vaccines. however, worldwide immunization rates are low. the two most likely reasons are the lack of sufficient distribution in underdeveloped countries and the high cost of vaccine products. the high costs are due to the difficulties of manufacturing individual vaccine products with specialized purification trains. in this study, we propose to use virus flocculation in osmolytes, fo ... | 2015 | 25865274 |
| in vitro antiviral activity of germacrone against porcine parvovirus. | porcine parvovirus (ppv) infections can lead to significant losses to the swine industry by causing reproductive failure in pigs. germacrone has been reported to efficiently suppress the replication of influenza virus. in this report, the antiviral activity of germacrone on ppv in swine testis (st) cells was investigated. here, we show for the first time that germacrone protects cells from ppv infection and suppresses the synthesis of viral mrna and protein. furthermore, we show that germacrone ... | 2015 | 25813663 |
| clinical utility of urinary cytology to detect bk viral nephropathy. | reactivation of bk polyoma virus can result in destructive viral allograft nephropathy (bkvan) with limited treatment options. screening programs using surrogate markers of viral replication are important preventive strategies, guiding immunosuppression reduction. | 2015 | 25769077 |
| parapoxvirus (ppv) of red deer reveals subclinical infection and confirms a unique species. | parapoxvirus (ppv) infections are of worldwide importance, particularly in sheep and goat herds. owing to the zoonotic potential of all ppv species, they are a permanent threat to human health as well. the virus is also known to affect wildlife, as reported for pinnipeds, red deer and several other wild ruminants. ppvs found in red deer have been claimed as a unique species according to certain genomic features. so far infection of wildlife has been recognized because of clinical manifestation s ... | 2015 | 25701822 |
| effect of plum pox virus on chemical composition and fruit quality of plum. | the quality of fruit from ppv (plum pox virus)-infected plum (prunus domestica l.) trees was investigated during the last 3 weeks of ripening using healthy tree (t3), tree with short-term infection (t2), and tree with long-term infection (t1). the pomological variables (presence of necrosis on fruit, color of fruit, color of flesh, firmness, soluble solids content, and fruit weight) and composition of nutritive compounds (sugars and organic acids) and bioactive compounds (phenolics: anthocyanins ... | 2015 | 25495040 |
| the p25 protein of potato virus x (pvx) is the main pathogenicity determinant responsible for systemic necrosis in pvx-associated synergisms. | most plant viruses counter the rna silencing-based antiviral defense by expressing viral suppressors of rna silencing (vsrs). in this sense, vsrs may be regarded as virulence effectors that can be recognized by the host as avirulence (avr) factors to induce r-mediated resistance. we made use of agrobacterium-mediated transient coexpression of vsrs in combination with potato virus x (pvx) to recapitulate in local tissues the systemic necrosis (sn) caused by pvx-potyvirus synergistic infections in ... | 2015 | 25473046 |
| sharka: how do plants respond to plum pox virus infection? | plum pox virus (ppv), the causal agent of sharka disease, is one of the most studied plant viruses, and major advances in detection techniques, genome characterization and organization, gene expression, transmission, and the description of candidate genes involved in ppv resistance have been described. however, information concerning the plant response to ppv infection is very scarce. in this review, we provide an updated summary of the research carried out to date in order to elucidate how plan ... | 2015 | 25336685 |
| mechanistic evaluation of virus clearance by depth filtration. | virus clearance by depth filtration has not been well-understood mechanistically due to lack of quantitative data on filter charge characteristics and absence of systematic studies. it is generally believed that both electrostatic interactions and sized based mechanical entrapment contribute to virus clearance by depth filtration. in order to establish whether the effectiveness of virus clearance correlates with the charge characteristics of a given depth filter, a counter-ion displacement techn ... | 2015 | 25683459 |
| limits in virus filtration capability? impact of virus quality and spike level on virus removal with xenotropic murine leukemia virus. | virus filtration (vf) is a key step in an overall viral clearance process since it has been demonstrated to effectively clear a wide range of mammalian viruses with a log reduction value (lrv) > 4. the potential to achieve higher lrv from virus retentive filters has historically been examined using bacteriophage surrogates, which commonly demonstrated a potential of > 9 lrv when using high titer spikes (e.g. 10(10) pfu/ml). however, as the filter loading increases, one typically experiences sign ... | 2015 | 25395156 |
| assessing the mismatch between incubation and latent periods for vector-borne diseases: the case of sharka. | the relative durations of the incubation period (the time between inoculation and symptom expression) and of the latent period (the time between inoculation and infectiousness of the host) are poorly documented for plant diseases. however, the extent of asynchrony between the ends of these two periods (i.e., their mismatch) can be a key determinant of the epidemic dynamics for many diseases and consequently it is of primary interest in the design of disease management strategies. in order to ass ... | 2015 | 26512749 |
| first identification of porcine parvovirus 6 in north america by viral metagenomic sequencing of serum from pigs infected with porcine reproductive and respiratory syndrome virus. | currently, eight species in four genera of parvovirus have been described that infect swine. these include ungulate protoparvovirus 1 (classical porcine parvovirus, ppv), ungulate tetraparvovirus 2 (ppv3), ungulate tetraparvovirus 3 (which includes ppv2, porcine hokovirus, porcine partetravirus and porcine parv4), ungulate copiparvovirus 2 (which includes ppv4 and ppv5), ungulate bocaparvovirus 2 (which includes porcine bocavirus 1, 2 and 6), ungulate bocaparvovirus 3 (porcine bocavirus 5), ungu ... | 2015 | 26475593 |
| molecular epidemiology and evolution of porcine parvoviruses. | porcine parvovirus (ppv), recently named ungulate protoparvovirus 1, is considered to be one of the most important causes of reproductive failure in swine. fetal death, mummification, stillbirths and delayed return to estrus are predominant clinical signs commonly associated with ppv infection in a herd. it has recently been shown that certain parvoviruses exhibit a nucleotide substitution rate close to that commonly determined for rna viruses. however, the ppv vaccines broadly used in the last ... | 2015 | 26453771 |
| aphid transmission of the ontario isolate of plum pox virus. | utilization of timed virus acquisition access probes in studies of plum pox virus (ppv) transmission by aphids demonstrated that endemic species transmitted the virus readily from plum, prunus domestica (l.) batsch; peach, p. persica (l.); or dwarf flowering almond, p. glandulosa thunberg., to peach seedlings. the green peach aphid, myzus persicae (sulzer), was shown to be the most efficient vector. acquisition of virus by green peach aphids from infected peach leaves resulted in 18-28% infected ... | 2015 | 26453705 |
| a prospective study on the predictive value of plasma bk virus-dna load for hemorrhagic cystitis in pediatric patients after stem cell transplantation. | in hematopoietic stem cell transplantation (hsct), late hemorrhagic cystitis (hc) has been associated with bk virus (bkv) infection. we assessed the value of plasma bkv load in predicting hc. | 2015 | 26407413 |
| the pathogenic role of torque teno sus virus 1 and 2 and their correlations with various viral pathogens and host immunocytes in wasting pigs. | the pathogenic role of torque teno sus virus (ttsuv) in swine is controversial among different studies. the present study intended to evaluate the potential pathogenicity of ttsuv based on its correlations with the histopathological changes, various common concurrently infected viral pathogens including porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), and porcine parvovirus (ppv), as well as changes in the distribution and population of host immunocy ... | 2015 | 26390821 |
| serological prevalence of viral agents that induce reproductive failure in south korean wild boar. | viral agents associated with reproductive failure such as aujeszky's disease virus (adv), encephalomyocarditis virus (emcv), and porcine parvovirus (ppv) have also been identified in european wild boar. to screen for the presence of antibodies against adv, emcv, and ppv from wild boar (sus scrofa) in south korea, 481 serum samples were collected from wild boar hunted between december 2010 and may 2011. | 2015 | 25888836 |
| simultaneous detection of eight swine reproductive and respiratory pathogens using a novel gexp analyser-based multiplex pcr assay. | a new high-throughput genomelab gene expression profiler (gexp) analyser-based multiplex pcr assay was developed for the detection of eight reproductive and respiratory pathogens in swine. the reproductive and respiratory pathogens include north american porcine reproductive and respiratory syndrome virus (prrsv-na), classical swine fever virus (csfv), porcine circovirus 2 (pcv-2), swine influenza virus (siv) (including h1 and h3 subtypes), porcine parvovirus (ppv), pseudorabies virus (prv) and ... | 2015 | 26259690 |
| the potyviridae p1a leader protease contributes to host range specificity. | the p1a protein of the ipomovirus cucumber vein yellowing virus is one of the self-cleavage serine proteases present in potyviridae family members. p1a is located at the n-terminal end of the viral polyprotein, and is closely related to potyviral p1 protease. for its proteolytic activity, p1a requires a still unknown host factor; this might be linked to involvement in host specificity. here we built a series of constructs and chimeric viruses to help elucidate the role of p1a cleavage in host ra ... | 2015 | 25562450 |
| shedding patterns of endemic eurasian wild boar (sus scrofa) pathogens. | the eurasianwild boar has experienced aworldwide demographic explosion that increases awareness on shared pathogens. however, shedding routes of relevant wild boar pathogens are unknown. previous observations on sex- and age-related differences in aujeszky's disease virus (adv) exposure led us to hypothesize that shedding patterns of endemicwild boar pathogens may be influenced by individual traits.we investigated shedding routes of adv, porcine parvovirus (ppv), porcine circovirus type 2 (pcv2) ... | 2015 | 26412545 |
| assessment of non-invasive models for liver fibrosis in chronic hepatitis b virus related liver disease patients in resource limited settings. | a total of 350 million individuals are affected by chronic hepatitis b virus infection world-wide. historically, liver biopsy has been instrumental in adequately assessing patients with chronic liver disease. a number of non-invasive models have been studied world-wide. | 2015 | 24152493 |
| coding algorithms for identifying patients with cirrhosis and hepatitis b or c virus using administrative data. | despite the use of administrative data to perform epidemiological and cost-effectiveness research on patients with hepatitis b or c virus (hbv, hcv), there are no data outside of the veterans health administration validating whether international classification of disease, ninth revision, clinical modification (icd-9-cm) codes can accurately identify cirrhotic patients with hbv or hcv. the validation of such algorithms is necessary for future epidemiological studies. | 2015 | 25335773 |
| the validation of the 2010 american association for the study of liver diseases guideline for the diagnosis of hepatocellular carcinoma in an endemic area. | hepatocellular carcinoma (hcc) diagnosis could be made with one typical imaging study in a cirrhotic liver by the guideline of the american association for the study of liver diseases (aasld) in 2010. patients with hepatitis b who may not have fully developed cirrhosis could be applied. we aim to retrospectively analyze and validate the diagnostic power of the 2010 guideline in an hcc endemic area (taiwan). | 2015 | 25092265 |
| evaluation of alpha-fetoprotein as a screening marker for hepatocellular carcinoma in hepatitis prevalent areas. | the objective of this study was to establish modified cutoff values of serum alpha-fetoprotein (afp) according to hepatitis status. while afp is used as a serum marker in the diagnosis or monitoring of hepatocellular carcinoma (hcc), its use as a screening method to the general population is controversial. we evaluated its screening performance in a hepatitis prevalent east asian population, and suggest different cutoff values according to the individual's hepatitis status. we evaluated the perf ... | 2015 | 26436360 |
| combined serum biomarkers in non-invasive diagnosis of non-alcoholic steatohepatitis. | non-alcoholic steatoheaptitis (nash), the critical stage of non-alcoholic fatty liver disease (nafld), is of chronic progression and can develop cirrhosis even hepatocellular carcinoma (hcc). however, non-invasive biomarkers for nash diagnosis remain poorly applied in clinical practice. our study aims at testing the accuracy of the combination of cytokeratin-18 m30 fragment (ck-18-m30), fibroblast growth factor 21 (fgf-21), interleukin 1 receptor antagonist (il-1ra), pigment epithelium-derived f ... | 2015 | 26121037 |
| non-injected illicit drug use and infectious disease risk of donor tissue: a single institution retrospective review. | this study assessed the relationship of non-injected illicit drug use and infectious disease seropositivity for human immunodeficiency virus (hiv-1), hepatitis b virus (hbv), hepatitis c virus (hcv), and syphilis. in a retrospective review of 986 donor charts recovered from 2009 to 2011 at a single tissue bank, the absence of reported non-injected illicit drug use corresponded with seropositivity in 6.61 %, of recovered donors while reported illicit drug use in the medical and social history cor ... | 2015 | 26006785 |
| serum levels of hepatitis b surface antigen predict severity of fibrosis in patients with e antigen-positive chronic hepatitis b. | noninvasive techniques are needed to assess hepatic fibrosis in patients with chronic hepatitis b. we developed a scoring system to determine the degree of fibrosis in patients with genotype b or genotype c hepatitis b virus (hbv) infection and positive for the hepatitis b e antigen. | 2015 | 25542306 |
| phase ii study of personalized peptide vaccination with both a hepatitis c virus-derived peptide and peptides from tumor-associated antigens for the treatment of hcv-positive advanced hepatocellular carcinoma patients. | objective. to evaluate safety and immune responses of personalized peptide vaccination (ppv) for hepatitis c virus- (hcv-) positive advanced hepatocellular carcinoma (hcc). patients and methods. patients diagnosed with hcv-positive advanced hcc were eligible for this study. a maximum of four hla-matched peptides were selected based on the preexisting igg responses specific to 32 different peptides, which consisted of a single hcv-derived peptide at core protein positions 35-44 (c-35) and 31 pept ... | 2015 | 26539554 |
| rapid prediction of treatment futility of boceprevir with peginterferon-ribavirin for taiwanese treatment experienced hepatitis c virus genotype 1-infected patients. | the efficacy and safety of the boceprevir (boc)-containing triple therapy in taiwanese treatment-experienced patients remains elusive. after 4 weeks of peginterferon/ribavirin lead-in therapy, patients with cirrhosis or previous null-response received triple therapy for 44 weeks; whereas others received 32 weeks of triple therapy followed by 12 weeks of peginterferon/ribavirin therapy. patients with hcv rna > 100 iu/ml at week 12 or with detectable hcv rna at week 24 of treatment were viewed as ... | 2015 | 26368130 |
| the clinical significance of hcv core antigen detection during telaprevir/peg-interferon/ribavirin therapy in patients with hcv 1 genotype infection. | direct-acting antiviral drugs (daa) regimen improve the svr rate. however, adverse effects often lead to therapy interruption. this underlines the importance to find some predictive parameters of response in order to consider the possibility of a shorter time of antiviral treatment in the appearance of adverse effects without affecting the success of the therapy. | 2015 | 26209382 |
| clinical characteristics and biopsy accuracy in suspected cases of sjögren's syndrome referred to labial salivary gland biopsy. | labial salivary gland biopsy (lsgb) is the most important diagnostic tool for the diagnosis of sjögren's syndrome (ss), but its diagnostic value is rarely studied. this study assessed the sensibility and specificity of lsgb, and the clinical profiles of patients who were referred for biopsy. | 2015 | 25887888 |
| utility of hepatitis c viral load monitoring on direct-acting antiviral therapy. | hepatitis c virus (hcv) rna loads serve as predictors of treatment response during interferon-based therapy. we evaluated the predictive ability of hcv rna levels at end of treatment (eot) for sustained virologic response (svr12) during interferon-sparing direct-acting antiviral therapies. | 2015 | 25733369 |
| concordance of sustained virological response 4, 12, and 24 weeks post-treatment with sofosbuvir-containing regimens for hepatitis c virus. | historically, clinical trials of regimens to treat chronic infection with hepatitis c virus (hcv) have used, as their primary efficacy endpoint, a sustained virological response (svr)—defined as hcv rna levels below a designated threshold of quantification—24 weeks after the end of treatment (svr24). more recently, regulatory authorities have begun to accept svr at 12 weeks post-treatment (svr12) as a valid efficacy endpoint because of its high rate of concordance with svr24. however, the concor ... | 2015 | 25314116 |
| antiviral effect of lithium chloride on infection of cells by porcine parvovirus. | porcine parvovirus (ppv) causes reproductive failure in pigs, which leads to economic losses to the industry. as reported previously, licl efficiently impairs the replication of a variety of viruses, including the coronavirus infectious bronchitis virus (ibv), transmissible gastroenteritis virus (tgev), and pseudorabies herpesvirus. we demonstrate for the first time that inhibition of ppv replication in swine testis (st) cells by licl is dose-dependent, and that the antiviral effect of licl occu ... | 2015 | 25663217 |
| self-reported hepatitis a vaccination as a predictor of hepatitis a virus antibody protection in u.s. adults: national health and nutrition examination survey 2007-2012. | to estimate the predictive value of self-reported hepatitis a vaccine (hepa) receipt for the presence of hepatitis a virus (hav) antibody (anti-hav) from either past infection or vaccination, as an indicator of hav protection. | 2015 | 26116252 |
| clinical application evaluation of two fourth-generation human immunodeficiency virus (hiv) screening assays in west china hospital. | fourth-generation human immunodeficiency virus (hiv) screening assays have been used in many laboratories. the elecsys® hiv combi pt assay is a new kind of fourth-generation hiv screening assay developed to allow earlier detection of seroconversion. | 2015 | 24797498 |
| parallel enzyme-linked immunosorbent assay screening for human immunodeficiency virus among blood donors in five chinese blood centres: a retrospective analysis. | to evaluate the strategy of parallel screening with different enzyme-linked immunosorbent assays (elisa) among chinese blood donors. | 2015 | 26104756 |
| use of real time polymerase chain reaction for detection of m. tuberculosis, m. avium and m. kansasii from clinical specimens. | the incidence of m. tuberculosis (mtb) and non tuberculous mycobacterium species (ntms) like m. avium and m. kansasii has increased due to human immunodeficiency virus (hiv) epidemic. therefore accurate, rapid and cost effective methods for the identification of these ntms and mtb are greatly needed for appropriate tb management. thus in this study we evaluated the performance of lightcycler(®) mycobacterium detection assay to detect mtb, m. avium and m. kansasii in sputum specimens. | 2015 | 25884439 |
| development and validation of new diagnostic criteria for acute retinal necrosis. | the purposes of this study are to develop and validate new diagnostic criteria for acute retinal necrosis (arn) based on the ocular findings, clinical course, and virologic testing of intraocular fluids. | 2015 | 25492579 |
| usefulness of bronchoscopic probe-based confocal laser endomicroscopy in the diagnosis of pneumocystis jirovecii pneumonia. | probe-based confocal laser endomicroscopy (pcle) is a novel technique that provides in vivo microscopic imaging of the distal lung. we hypothesized that the intra-alveolar exudates characterizing pneumocystis jirovecii pneumonia (pjp) can be identified by pcle in vivo and help in its diagnosis. | 2016 | 27362271 |
| quantitative and functional antibody responses to the 13-valent conjugate and/or 23-valent purified polysaccharide vaccine in aging hiv-infected adults. | the number of aging human immunodeficiency virus-infected (hiv+) individuals living in the united states has substantially grown over the past two decades. advanced age and hiv infection both increase susceptibility to streptococcus pneumoniae infection due to b cell dysfunction. the combined impact of these factors on pneumococcal vaccine responses remains unknown. | 2016 | 27158552 |
| provocation proven drug allergy in thai children with adverse drug reactions. | adverse drug reactions (adrs) are a common healthcare problem. the drug provocation test (dpt) is a gold standard for adr diagnosis. | 2016 | 26994627 |
| alterations in serotype-specific b cell responses to the 13-valent pneumococcal conjugate vaccine in aging hiv-infected adults. | advanced age and human immunodeficiency virus (hiv) infection are associated with increased pneumococcal disease risk. the impact of these factors on cellular responses to vaccination is unknown. | 2016 | 26707220 |
| hepatitis c viral kinetic changes in a retrospective cohort study of chronic hepatitis c virus egyptian patients on pegylated interferon and ribavirin therapy. | the aim of this study was to determine the relative importance of the kinetics of antiviral response compared to baseline host and virological factors for predicting treatment outcome. a retrospective analysis of 285 chronic hepatitis c virus (hcv) patients, encompassing genotypes 4 treated with peginterferon alpha-2a and ribavirin, was performed. baseline characteristics were compared across hcv genotypes and pretreatment factors associated with rapid virological response (rvr) were identified. ... | 2016 | 26982165 |
| can epstein-barr virus dna load in nasopharyngeal brushings or whole blood predict recurrent nasopharyngeal carcinoma in a non-endemic region? a prospective nationwide study of the dutch head and neck oncology cooperative group. | this study estimated the value of quantitative measurements of ebv markers in the clinical management of nasopharyngeal carcinoma in a non-endemic area. the aim was to predict prognosis and detect recurrent and residual disease. in 72 patients, ebv dna load in blood and nasopharyngeal brushes, and iga vca-p18 and ebna1 in plasma were measured at different time points. at diagnosis and post-treatment, a cut-off value was used for detecting disease [positive (ppv) and negative (npv) predictive val ... | 2016 | 25929413 |
| comparison of directigen flu a+b with real time pcr in the diagnosis of influenza. | early diagnosis and treatment of patients with influenza is the reason why physicians need rapid high-sensitivity influenza diagnostic tests that require no complex lab equipment and can be performed and interpreted within 15 min. the aim of this study was to compare the rapid directigen flu a+b test with real time pcr for detection of influenza viruses in the republic of macedonia. | 2016 | 26933779 |
| molecular triage of premalignant lesions in liquid-based cervical cytology and circulating cell-free dna from urine, using a panel of methylated human papilloma virus and host genes. | clinically useful molecular tools to triage women for a biopsy upon referral to colposcopy are not available. we aimed to develop a molecular panel to detect cervical intraepithelial neoplasia (cin) grade 2 or higher lesions (cin2(+)) in women with abnormal cervical cytology and high-risk hpv (hpv(+)). we tested a biomarker panel in cervical epithelium dna obtained from 211 women evaluated in a cervical cancer clinic in chile from 2006 to 2008. results were verified in a prospective cohort of 10 ... | 2016 | 27671338 |
| a pilot study to compare the detection of hpv-16 biomarkers in salivary oral rinses with tumour p16(ink4a) expression in head and neck squamous cell carcinoma patients. | human papilloma virus-16 (hpv-16) infection is a major risk factor for a subset of head and neck squamous cell carcinoma (hnscc), in particular oropharyngeal squamous cell carcinoma (opscc). current techniques for assessing the hpv-16 status in hnscc include the detection of hpv-16 dna and p16(ink4a) expression in tumor tissues. when tumors originate from hidden anatomical sites, this method can be challenging. a non-invasive and cost-effective alternative to biopsy is therefore desirable for hp ... | 2016 | 26940728 |
| improving the performance of reflex human papilloma virus (hpv) testing in triaging women with atypical squamous cells of undetermined significance (ascus): a restrospective study in a tertiary hospital in united arab emirates (uae). | cervical cancer is the second commonest cancer in women worldwide. infection with oncogenic types of human papillomavirus (hpv) is the most important risk factor for developing cervical cancer. reflex high risk hpv (hr-hpv) testing is of significant value in the assessment of papanicolaou (pap) smear results where ascus are identified. | 2016 | 26747717 |
| utility of enzyme-linked immunosorbent assays to test core antigen in the diagnosis and antiviral therapy management of hepatitis c virus infections. | in this study, we evaluate the performance of the enzyme-linked immunosorbent assays (elisas) for hcv ag detection in the diagnosis and antiviral therapy management of hcv infections. for the diagnosis of an active hcv infection, the limit of detection of hcv ag corresponding to hcv rna level was approximately 7300 iu/ml; the sensitivity, specificity, positive predictive value (ppv), and negative predictive value (npv) of hcv-ag were 88.96, 100, 100, and 91.33%, respectively. the pearson's corre ... | 2016 | 27958657 |
| serological tests do not predict residual fibrosis in hepatitis c cirrhotics with a sustained virological response to interferon. | liver biopsy (lb) has lost popularity to stage liver fibrosis in the era of highly effective anti-hepatitis c virus (hcv) therapy, yet diagnosis of persistent cirrhosis may have important implications following hcv eradication. as performance of serological non-invasive tests (nits) to predict residual fibrosis in non-viremic hcv patients is unknown, we investigated accuracy of nits to predict residual fibrosis in cirrhotics after a sustained virological response (svr) to interferon (ifn). | 2016 | 27304619 |
| hcv ns3ag: a reliable and clinically useful predictor of antiviral outcomes in genotype 1b hepatitis c virus-infected patients. | since hepatitis c virus (hcv) non-structural 3 (ns3) protease inhibitor (pi) combined with pegylated interferon/ribavirin (pr) has been approved for chronic hcv genotype (gt) 1b infection, a reliable and clinically useful predictor combining with serum hcv rna to predict early virologic response, breakthrough, and relapse is important during hcv antiviral treatment. we evaluated the role of hcv ns3 antigen (hcv ns3ag) on the prediction of virologic response in patients with hcv gt1b during pr or ... | 2016 | 27173787 |
| on-treatment quantitative hepatitis b e antigen predicted response to nucleos(t)ide analogues in chronic hepatitis b. | to investigate potential predictors for treatment response to nucleos(t)ide analogues (nas) in hepatitis b e antigen (hbeag)-positive chronic hepatitis b (chb) patients. | 2016 | 28008342 |
| diagnostic accuracy of apri and fib-4 for predicting hepatitis b virus-related liver fibrosis accompanied with hepatocellular carcinoma. | aspartate aminotransferase to platelet ratio index (apri) and the fibrosis index based on four factors (fib-4) are the two most focused non-invasive models to assess liver fibrosis. | 2016 | 27599803 |
| multiplex reverse transcriptase-pcr for simultaneous detection of hepatitis b, c, and e virus. | the hepatitis b virus (hbv), hcv, and hev may occur as singly or concurrently in patients of different kind of liver disease. the rapid, reliable, and cost-effective screening of these pathogens is required for the large epidemiological studies. therefore, a study has been planned to develop a multiplex reverse transcriptase-pcr assay which can be used for the screening of maximum number of pathogens at a time. | 2016 | 27194894 |
| immunogenicity of adenovirus-derived porcine parvovirus-like particles displaying b and t cell epitopes of foot-and-mouth disease. | virus-like particles (vlps) vaccines combine many of the advantages of whole-virus vaccines and recombinant subunit vaccines, integrating key features that underlay their immunogenicity, safety and protective potential. we have hypothesized here the effective insertion of the vp1 epitopes (three amino acid residues 21-40, 141-160 and 200-213 in vp1, designated vpe) of foot-and-mouth disease (fmdv) within the external loops of ppv vp2 could be carried out without altering assembly based on struct ... | 2016 | 26685093 |
| logiq e9 shear wave elastrography for detection of liver fibrosis in patients with chronic hepatitis c virus. | shear wave elastography (swe) is an emerging technology that assesses hepatic fibrosis. the aim of our study was to determine the diagnostic accuracy of general electric (ge) healthcare's logiq e9 swe in patients with chronic hepatitis c virus using liver biopsy as the reference standard. | 2016 | 27812720 |
| hepatitis e virus derived from different sources exhibits different behaviour in virus inactivation and/or removal studies with plasma derivatives. | hepatitis e virus (hev) causes viral hepatitis, and is considered a risk factor for blood products. although some hev inactivation/removal studies have been reported, detailed investigations of different manufacturing steps as heat treatment, partitioning during cold ethanol fractionation, low ph treatment, and virus filtration have yet to be reported for plasma-derived medicinal products. in this study, human serum- and swine faeces-derived hevs, with and without detergent treatment, were used. ... | 2016 | 27461242 |
| simultaneous typing of seven porcine pathogens by multiplex pcr with a gexp analyser. | a novel high-throughput method was developed for simultaneous detection and differentiation of seven porcine pathogens by multiplex pcr based on a genomelab gene expression profiler (gexp) analyser. the pathogens included in this study were pseudorabies virus (prv), classical swine fever virus (csfv), african swine fever virus (asfv), porcine reproductive and respiratory syndrome virus (prrsv), porcine parvovirus (ppv), porcine circovirus type 2 (pcv-2) and japanese encephalitis virus (jev). sev ... | 2016 | 26706731 |
| rapid and specific detection of porcine parvovirus by isothermal recombinase polymerase amplification assays. | porcine parvovirus (ppv) is a major cause of swine reproductive failure and reported in many countries worldwide. recombinase polymerase amplification (rpa) assays using a real-time fluorescent detection (ppv real-time rpa assay) and a lateral flow dipstick (ppv rpa lfd assay) were developed targeting ppv ns1 gene. the detection limit of ppv real-time rpa assay was 300 copies per reaction within 9 min at 38 °c, while the rpa lfd assay has a detection limit of 400 copies per reaction in less than ... | 2016 | 27593155 |
| establishment of a nanoparticle-assisted rt-pcr assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus. | porcine epidemic diarrhea virus (pedv) can cause serious disease and even death in neonatal piglets, resulting in serious damage to the swine industry worldwide. open reading frame 3 (orf3) is the only accessory gene in the pedv genome. previous studies have indicated that pedv vaccine strains have a partial deletion in orf3. in this study, a nanoparticle-assisted polymerase chain reaction (nanoparticle-assisted rt-pcr) assay targeting the orf3 of pedv was developed to distinguish pedv field str ... | 2016 | 27287433 |
| diagnostic potential of monoclonal antibodies against the capsid protein of chikungunya virus for detection of recent infection. | chikungunya fever is self-limiting. however, neurological and hemorrhagic complications have been seen in recent outbreaks. the clinical manifestations of this disease are similar to those of dengue virus infection, indicating the need for differential diagnosis in areas such as india, which are endemic for both viruses. the aim of the present study was to develop monoclonal antibodies (mabs) against chikungunya virus (chikv) and assess their use in mab-based igm capture elisa (mac elisa). the e ... | 2016 | 27016930 |
| genome-wide association links candidate genes to resistance to plum pox virus in apricot (prunus armeniaca). | in fruit tree species, many important traits have been characterized genetically by using single-family descent mapping in progenies segregating for the traits. however, most mapped loci have not been sufficiently resolved to the individual genes due to insufficient progeny sizes for high resolution mapping and the previous lack of whole-genome sequence resources of the study species. to address this problem for plum pox virus (ppv) candidate resistance gene identification in prunus species, we ... | 2016 | 26356603 |
| combining billing codes, clinical notes, and medications from electronic health records provides superior phenotyping performance. | to evaluate the phenotyping performance of three major electronic health record (ehr) components: international classification of disease (icd) diagnosis codes, primary notes, and specific medications. | 2016 | 26338219 |
| evaluation of case definitions to detect respiratory syncytial virus infection in hospitalized children below 5 years in rural western kenya, 2009-2013. | in order to better understand respiratory syncytial virus (rsv) epidemiology and burden in tropical africa, optimal case definitions for detection of rsv cases need to be identified. | 2016 | 27207342 |
| diagnostic performance of influenza viruses and rsv rapid antigen detection tests in children in tertiary care. | rapid antigen detection tests (radts) are increasingly used to detect influenza viruses and respiratory syncytial virus (rsv). however, their sensitivity and specificity are a matter of debate, challenging their clinical usefulness. | 2016 | 27045454 |
| plum pox virus 6k1 protein is required for viral replication and targets the viral replication complex at the early stage of infection. | the potyviral rna genome encodes two polyproteins that are proteolytically processed by three viral protease domains into 11 mature proteins. extensive molecular studies have identified functions for the majority of the viral proteins. for example, 6k2, one of the two smallest potyviral proteins, is an integral membrane protein and induces the endoplasmic reticulum (er)-originated replication vesicles that target the chloroplast for robust viral replication. however, the functional role of 6k1, ... | 2016 | 26962227 |
| incidence of hospitalization for respiratory syncytial virus infection amongst children in ontario, canada: a population-based study using validated health administrative data. | rsv is a common illness among young children that causes significant morbidity and health care costs. | 2016 | 26958849 |
| an inactivated whole-virus porcine parvovirus vaccine protects pigs against disease but does not prevent virus shedding even after homologous virus challenge. | inactivated whole-virus vaccines against porcine parvovirus (ppv) can prevent disease but not infection and virus shedding after heterologous virus challenge. here, we showed that the same is true for a homologous challenge. pregnant sows were vaccinated with an experimental inactivated vaccine based on ppv strain 27a. they were challenged on day 40 of gestation with the virulent porcine parvovirus ppv-27a from which the vaccine was prepared (homologous challenge). on day 90 of gestation, the fe ... | 2016 | 26939976 |
| porcine parvovirus as a contaminant in cell cultures and laboratory supplies. | although ppv has been described as a cellular contaminant, few recent studies about the presence of this virus in cell cultures, serum, and trypsin were found in the literature. the purpose of this study was to detect the presence of porcine parvovirus (ppv) by polymerase chain reaction (pcr) in cell cultures, serum, and trypsin used in official public laboratories of educational institutes and research centers. we tested samples of cell cultures (88), batches of trypsin (10), and fetal bovine s ... | 2016 | 26811218 |
| a rapid protocol of crude rna/dna extraction for rt-qpcr detection and quantification of 'candidatus phytoplasma prunorum'. | many efforts have been made to develop a rapid and sensitive method for phytoplasma and virus detection. taking our cue from previous works, different rapid sample preparation methods have been tested and applied to candidatus phytoplasma prunorum ('ca. p. prunorum') detection by rt-qpcr. a duplex rt-qpcr has been optimized using the crude sap as a template to simultaneously amplify a fragment of 16s rrna of the pathogen and 18s rrna of the host plant. the specific plant 18s rrna internal contro ... | 2016 | 26742106 |
| genome sequence analysis of new isolates of the winona strain of plum pox virus and the first definitive evidence of intrastrain recombination events. | plum pox virus (ppv) is genetically diverse with nine different strains identified. mutations, indel events, and interstrain recombination events are known to contribute to the genetic diversity of ppv. this is the first report of intrastrain recombination events that contribute to ppv's genetic diversity. fourteen isolates of the ppv strain winona (w) were analyzed including nine new strain w isolates sequenced completely in this study. isolates of other strains of ppv with more than one isolat ... | 2016 | 26667187 |
| epidemiological and immunopathological studies on porcine parvovirus infection in punjab. | the aim of this study was to get the first-hand knowledge about the seroprevalence of porcine parvovirus (ppv) in punjab and a diagnosis of ppv from abortion cases of swine using gross, histopathological, and immunohistopathological techniques to observe the tissue tropism of the virus strain. | 2016 | 27651669 |
| fighting sharka in peach: current limitations and future perspectives. | sharka, caused by plum pox virus (ppv), is by far the most important infectious disease of peach [p. persica (l.) batsch] and other prunus species. the progressive spread of the virus in many important growing areas throughout europe poses serious issues to the economic sustainability of stone fruit crops, peach in particular. the adoption of internationally agreed-upon rules for diagnostic tests, strain-specific monitoring schemes and spatial-temporal modeling of virus spread, are all essential ... | 2016 | 27625664 |
| new insights into the history of domesticated and wild apricots and its contribution to plum pox virus resistance. | studying domesticated species and their wild relatives allows understanding of the mechanisms of population divergence and adaptation, and identifying valuable genetic resources. apricot is an important fruit in the northern hemisphere, where it is threatened by the plum pox virus (ppv), causing the sharka disease. the histories of apricot domestication and of its resistance to sharka are however still poorly understood. we used 18 microsatellite markers to genotype a collection of 230 wild tree ... | 2016 | 27480465 |
| complete genome sequence of a novel potyvirus, callistephus mottle virus, identified in callistephus chinensis. | the complete genomic sequence of a novel putative member of the genus potyvirus was detected from callistephus chinensis (china aster) in south korea. the genomic rna consists of 9,859 nucleotides (excluding the 3' poly(a) tail) and contains the typical open reading frame of potyviruses, encoding a putative large polyprotein of 3,154 amino acids. the callistephus virus is most closely related to plum pox virus and members of the apvy subgroup which showed 50-52 % polyprotein amino acid sequence ... | 2016 | 27480416 |
| genotyping by sequencing (gbs) in apricots and genetic diversity assessment with gbs-derived single-nucleotide polymorphisms (snps). | genotyping by sequencing (gbs), which is a highly promising technique for molecular breeding, has been implemented in apricots, including turkish, european, and plum pox virus-resistant accessions. dna samples were digested with the apeki restriction enzyme to construct a genome-complexity-reduced 90-plex gbs library. after filtering the raw sequences, approximately 28 g of clean data were generated, and 17,842 high-quality single-nucleotide polymorphism (snp) loci were discovered. a total of 56 ... | 2016 | 27465591 |
| should viral load thresholds be lowered?: revisiting the who definition for virologic failure in patients on antiretroviral therapy in resource-limited settings. | the world health organization (who) guidelines on antiretroviral therapy (art) define treatment failure as 2 consecutive viral loads (vls) ≥1000 copies/ml. there is, however, little evidence supporting 1000 copies as an optimal threshold to define treatment failure. objective of this study was to assess the correlation of the who definition with the presence of drug-resistance mutations in patients who present with 2 consecutive unsuppressed vl in a resource-limited setting.in 10 nurse-led clini ... | 2016 | 27428189 |
| [immune response of recombinant pseudorabies virus rprv-vp2 expressing vp2 gene of porcine parvovirus in mice]. | in order to develop a combined live vaccine that will be used to prevent against porcine parvovirus (ppv) and pseudorabies virus (prv) infection, the vp2 gene of ppv was inserted into the transfer vector plasmid pg to produce the recombinant plasmid pgvp2. the plasmid pgvp2 and the genome of prv hb98 attenuated vaccine were transfected by using lipofectamine into swine testis cells for the homologous recombination. the recombinant virus rprv-vp2 was purified by selection of green fluorescence pl ... | 2016 | 27396164 |
| validity of the coding for herpes simplex encephalitis in the danish national patient registry. | large health care databases are a valuable source of infectious disease epidemiology if diagnoses are valid. the aim of this study was to investigate the accuracy of the recorded diagnosis coding of herpes simplex encephalitis (hse) in the danish national patient registry (dnpr). | 2016 | 27330328 |
| plum pox virus capsid protein suppresses plant pathogen-associated molecular pattern (pamp)-triggered immunity. | the perception of pathogen-associated molecular patterns (pamps) by immune receptors launches defence mechanisms referred to as pamp-triggered immunity (pti). successful pathogens must suppress pti pathways via the action of effectors to efficiently colonize their hosts. so far, plant pti has been reported to be active against most classes of pathogens, except viruses, although this defence layer has been hypothesized recently as an active part of antiviral immunity which needs to be suppressed ... | 2016 | 27301551 |
| removal process of prion and parvovirus from human platelet lysates used as clinical-grade supplement for ex vivo cell expansion. | pooled human platelet lysate (hpl) is becoming the new gold standard as supplement for ex vivo cell culture for clinical protocols. however, the risk of pathogen contamination of hpl increases with the platelet pool size. we hypothesized that hollow fiber anion exchange membrane chromatography using qyuspeed d (qsd) could remove resistant and untested bloodborne pathogens, such as parvoviruses and prions, from hpl-supplemented growth media without substantially affecting their capacity to suppor ... | 2016 | 27260210 |
| occurrence and characterization of plum pox virus strain d isolates from european russia and crimea. | numerous plum pox virus (ppv) strain d isolates have been found in geographically distant regions of european russia and the crimean peninsula on different stone fruit hosts. phylogenetic analysis of their partial and complete genomes suggests multiple introductions of ppv-d into russia. distinct natural isolates from prunus tomentosa were found to bear unique amino acid substitutions in the n-terminus of the coat protein (cp) that may contribute to the adaptation of ppv-d to this host. serologi ... | 2016 | 26530833 |
| persistent corneal epithelial defect after pars plana vitrectomy. | to investigate the incidence, risk factors, and clinical course of persistent corneal epithelial defects (pced) after pars plana vitrectomy (ppv). | 2016 | 26166798 |
| utility of keywords from chest radiograph reports for pneumonia surveillance among hospitalized patients with influenza: the cdc influenza hospitalization surveillance network, 2008-2009. | transcripts from admission chest radiographs could aid in identification of pneumonia cases for public health surveillance. we assessed the reliability of radiographic data abstraction and performance of radiographic key terms to identify pneumonia in patients hospitalized with laboratory-confirmed influenza virus infection. | 2017 | 27252568 |
| sugars and organic acids in plum fruit affected by plum pox virus. | plum pox virus (ppv) causes severe economic losses in stone fruit production, but little is known about its effect on plum fruit composition. in this study, the influence of ppv on sugars and organic acids was evaluated in a susceptible plum (prunus domestica l.) cultivar. | 2017 | 27614092 |
| an efficient viral vector for functional genomic studies of prunus fruit trees and its induced resistance to plum pox virus via silencing of a host factor gene. | rna silencing is a powerful technology for molecular characterization of gene functions in plants. a commonly used approach to the induction of rna silencing is through genetic transformation. a potent alternative is to use a modified viral vector for virus-induced gene silencing (vigs) to degrade rna molecules sharing similar nucleotide sequence. unfortunately, genomic studies in many allogamous woody perennials such as peach are severely hindered because they have a long juvenile period and ar ... | 2017 | 27565765 |
| virulence determines beneficial trade-offs in the response of virus-infected plants to drought via induction of salicylic acid. | it has been hypothesized that plants can get beneficial trade-offs from viral infections when grown under drought conditions. however, experimental support for a positive correlation between virus-induced drought tolerance and increased host fitness is scarce. we investigated whether increased virulence exhibited by the synergistic interaction involving potato virus x (pvx) and plum pox virus (ppv) improves tolerance to drought and host fitness in nicotiana benthamiana and arabidopsis thaliana. ... | 2017 | 28718885 |
| development of a novel rapid micro-neutralization elisa for the detection of neutralizing antibodies against chandipura virus. | chandipura virus (chpv) is a leading cause of acute encephalitis with high mortality in paediatric population in india. a micro-neutralization elisa (mn-elisa) assay was developed for the detection of neutralizing antibodies (nab) against chpv. this novel method gives read-out in the form of elisa optical density (od) values and has a shorter turn-around time (tat) as compared to the conventional cytopathic effect (cpe)-based neutralization assay (mn-cpe). the assay was developed using an indian ... | 2017 | 27856212 |
| do two screening tools for chikungunya virus infection that were developed among younger population work equally as well in patients aged over 65 years? | chikungunya is an endemo-epidemic infection, which is still considered as an emerging public health problem. the aim of this study was to evaluate in a 65+ population, the accuracy of two chikungunya screening scores that were developed in younger people. | 2017 | 28056031 |
| porcine parvovirus capsid protein expressed in escherichia coli self-assembles into virus-like particles with high immunogenicity in mice and guinea pigs. | porcine parvovirus (ppv) is a causative agent of reproductive failure in pregnant sows. classical inactivated vaccine is extensively used to control ppv infection, but problems concerning safety, such as incomplete inactivation may occur. in this study, a novel subunit vaccine against ppv based on virus-like particles (vlps) formed from the complete ppv vp2 protein expressed in a prokaryotic system with co-expressed chaperones is reported. the vlps have a similar size, shape, and hemagglutinatio ... | 2017 | 28063996 |
| laboratory and molecular characterization of dengue virus during the 2014 outbreak in guangfo region, southern china. | non-specific symptoms and low viremia levels make early diagnosis of dengue virus (denv) infection challenging. this study aimed to 1) identify laboratory markers which can predict a positive laboratory diagnosis, and 2) perform a molecular characterization of denv from the 2014 guangdong epidemic. this retrospective study enrolled 1044 patients from the guangdong epidemic who had clinically suspected dengue. viral rna was detected by real-time rt-pcr and viral-specific ns1 antigen was detected ... | 2017 | 28367875 |
| a combination of different diagnostic tools allows identification of inactive hepatitis b virus carriers at a single time point evaluation. | serial evaluation of hepatitis b virus (hbv) dna and aminotransferase values is required for identification of inactive hbv carriers (ics). recently, hbv surface antigen quantification (qhbsag) and liver stiffness measurement (lsm) have been proposed as diagnostic tools in chronic hbv infection. the aim of this study was to evaluate the efficacy of hbv dna quantification, qhbsag and lsm in diagnosing ics at a single time point. | 2017 | 27606573 |
| a novel system for predicting liver histopathology in patients with chronic hepatitis b. | there is currently a lack of reliable, reproducible, and easily applied methods for assessing changes in liver histology in patients in the gray zone phase of chronic hepatitis b (chb). therefore, we aimed to develop a novel predictive scoring system to detect significant liver histological changes in these patients.a total of 388 patients in the gray zone phase of chb who underwent liver biopsy were divided into a training group and a validation group, and their clinical and routinely available ... | 2017 | 28383410 |
| predictors of liver histological changes and a sustained virological response to peginterferon among chronic hepatitis b e antigen-positive patients with normal or minimally elevated alanine aminotransferase levels. | a proportion of chronic hepatitis b patients with normal or only minimally elevated alanine aminotransferase (alt) levels display significant histologic changes and would benefit from antiviral therapy. we aim to evaluate the histologic abnormalities seen in these patients and then determine which of them would most likely respond to peginterferon therapy. one hundred and thirteen hepatitis b e antigen (hbeag)-positive patients with a normal or minimally elevated alt level and moderate-to-severe ... | 2017 | 28107601 |
| role of signal-to-cut-off ratios of anti-hepatitis c virus antibody by enzyme immunoassays along with id-nat for screening of whole blood donors in india. | the use of elevated signal-to-cut off ratios (s/co) as an alternate to further supplemental testing (i.e., riba) has been included in the guidelines provided by the centres for disease control and prevention for hcv diagnostic purposes since 2003. with availability of screening by nat and non availability of riba, further confirmation of hcv infection has been possible at the molecular level (rna). | 2017 | 27011676 |
| hepatitis c virus core antigen assay: an alternative method for hepatitis c diagnosis. | background the study aimed to evaluate a fully automated chemiluminescent immunoassay and compared it with a quantitative rna assay and anti-hcv assay to verify the utility of this automated ag assay as an alternative method for hepatitis c diagnosis. methods a total of 229 serum samples previously tested for anti-hcv concentrations by the architect anti-hcv assay, were selected for hcv rna testing by real time rt-pcr kit (shanghai zj bio-tec co., ltd) and 125 specimens were tested for hcv ag by ... | 2017 | 27614354 |
| non-invasive assessment of liver fibrosis in patients with hepatitis c: shear wave elastography and colour doppler velocity profile technique versus liver biopsy. | determination of the presence and degree of liver fibrosis is essential for the prognosis and treatment of patients with chronic hepatitis c. non-invasive methods of assessing fibrosis have been developed to reduce the need for biopsy. we determined the efficacy of shear wave elastography (swe) and colour doppler velocity as non-invasive methods for the assessment of liver fibrosis compared to liver biopsy among patients with chronic hepatitis c virus (hcv) infection. | 2017 | 28262531 |
| predictors of extracapsular extension in hpv-associated oropharyngeal cancer treated surgically. | extracapsular extension (ece) in cervical metastatic lymph nodes remains an indication for adding chemotherapy for patients with oropharyngeal squamous cell carcinoma (opscc). the aim of this study is to identify specific imaging characteristics on computed tomography (ct) scan that are predictive of ece in order to better risk stratify patients preoperatively. | 2017 | 28109475 |