Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| hepatitis c virus replication during acute infection in the chimpanzee. | the events following experimental infection of 2 chimpanzees with the h strain of hepatitis c virus (hcv) were studied by quantitating the levels of hcv rna in liver and serum. serum and liver samples were tested every 1-3 weeks for up to 32 weeks. the genomic and antigenomic strands of hcv rna were individually detected in liver and serum by strand-specific reverse transcription followed by polymerase chain reaction (pcr) using nested primers specific for the 5' noncoding region of the hcv geno ... | 1992 | 1321863 |
| indeterminate second-generation hepatitis c recombinant immunoblot test: detection of hepatitis c virus infection by polymerase chain reaction. | 1992 | 1321866 | |
| evaluating recombinant protein immunoblot assay and polymerase chain reaction for diagnosis of non-a, non-b hepatitis. | 1992 | 1321867 | |
| the prevalence of hepatitis c in a regional level i trauma center population. | several studies have examined the prevalence of hepatitis b (hbv) and human immunodeficiency virus (hiv) in a trauma population. to our knowledge, no one has reported on the prevalence of hepatitis c (hcv). we prospectively studied the prevalence of hcv, as well as hbv, hiv, and syphilis in our adult regional level i trauma center population. two hundred eighty-six consecutive trauma patients were tested for previous exposure to hcv using an anti-hcv mab elisa. patients were also tested for expo ... | 1992 | 1321915 |
| heterogeneity within the nonstructural protein 5-encoding region of hepatitis c viruses from a single patient. | nucleotide (nt) sequence heterogeneity of the hepatitis c virus (hcv) genome derived from a single carrier was investigated. a polymerase chain reaction (pcr) product of 311 bp in the putative nonstructural protein 5-encoding region was directly sequenced, while part of a pcr product was cloned, and sequence analyses were carried out for 27 independent clones. although 14 of the 27 clones were conserved, ten other types of nt sequences were found. the difference was at most 3 nt (1.1%). a direct ... | 1992 | 1322345 |
| detection of hepatitis c virus by polymerase chain reaction and response to interferon-alpha therapy: relationship to genotypes of hepatitis c virus. | to investigate the relationship between genotypes of hepatitis c virus and response to interferon-alpha therapy, hepatitis c virus rna was assayed by polymerase chain reaction with three sets of primers and probes in 70 patients with non-a, non-b chronic hepatitis who received interferon-alpha. twenty-four patients sustained long-term remissions (complete responders). polymerase chain reaction for 5'-terminal noncoding region detected hepatitis c virus rna in 94.3% (66 of 70) of the patients. po ... | 1992 | 1322348 |
| presence of lower temperature-dependent antibody with low avidity to c100-3 (hcv) antigen in voluntary blood donors. | serum samples with lower temperature-dependent antibody with low avidity to c100-3 (hcv) antigen were found in 0.19% of 23,197 voluntary blood donors at this blood center. they showed positive c100-3 antibody activity at 24 c but not at 37 c. the antibody activity bound to c100-3 antigen at lower temperature disappeared after incubation for 60 min at 37 c or treatment with 8 m urea. other markers of hepatitis c virus infection, especially the presence of hcv-rna were demonstrated in some of thes ... | 1992 | 1322472 |
| [occurrence of antibodies against hepatitis c virus (hcv) among alcoholics]. | 144 consecutive alcoholics attending an outpatient clinic were tested for the prevalence of anti-hcv with commercially available elisa test (abbott hcv eia). positive sera were further tested with neutralization assay (abbott hcv neutralization assay). anti-hcv were found in 35 (24%). there was no difference in the prevalence or mean titre of anti-cmv and anti-hsv between patients with and without anti-hcv what points to the specificity of tested antibodies. | 1992 | 1322534 |
| [occurrence of antibodies against hepatitis c virus (hcv) among drug addicts]. | the prevalence of anti-hcv and serological markers of hbv infection was tested in 100 unselected intravenous drug addicts admitted to a detoxification ward. anti-hcv were present in 78% addicts, and at least one markers of hbv infection was present in 69% addicts. no correlation was found between anti-hcv status and age, gender, duration of addiction or anti-hiv presence. furthermore, there was no difference in mean titres of anti-cmv and anti-hsv between subjects with and without anti-hcv what ... | 1992 | 1322535 |
| the association of serum hepatitis c virus rna with serum aminotransferase activities. | serum samples from 71 patients with hepatitis c virus infection at various stages were studied to determine the clinical significance of the detection of hepatitis c virus rna by the polymerase chain reaction. there was a significant correlation of serum hcv rna with elevation of the serum aminotransferase levels. these data suggest that the detection of hcv rna in the serum might be useful for evaluation of the extent of ongoing liver damage. | 1992 | 1322560 |
| early antihepatitis c virus response with second-generation c200/c22 elisa. | detection of early antibody to hepatitis c virus (hcv) by a new second-generation c200/c22 anti-hcv enzyme-linked immunosorbent assay (elisa) and a four-antigen recombinant immunoblot assay (4-riba) was compared with the first-generation anti-hcv c100 elisa using sequential serum samples of 9 recipients who were infected with hcv, as detected by polymerase chain reaction after transfusion of blood products. within 26 weeks after transfusion, 9/9 (100%) recipients seroconverted with c200/22 elisa ... | 1992 | 1322611 |
| hepatitis c viraemia in united kingdom blood donors. a multicentre study. | of 10,633 blood donations tested in three regional blood transfusion centres with two commercial first generation screening assays for antibodies to the hepatitis c virus (hcv), 65 (0.61%) were found to be repeatedly reactive in one or both assays. five of the 65 were confirmed positive by recombinant immunoblot assay (ortho riba-2) and a further 4 were judged indeterminate. all 5 riba-2 positive donations and 1 of the 4 riba-2 indeterminates were shown to be viraemic by hcv-rna polymerase chain ... | 1992 | 1322612 |
| comparison of several pcr procedures for detection of serum hcv-rna using different regions of the hcv genome. | different methods for the isolation of hepatitis c virus rna and several sets of primers from the 5' untranslated, core, ns3, ns3/ns4 and ns5 regions of the hepatitis c virus genome were used for the detection of the viral genome by the polymerase chain reaction. serum samples from 10 patients with chronic hepatitis c and 10 healthy controls were studied. the best method for the rna extraction was with cold guanidinium isothiocyanate followed by a denaturation step prior to the polymerase chain ... | 1992 | 1322933 |
| at least five related, but distinct, hepatitis c viral genotypes exist. | hepatitis c virus, the major causative agent of blood-borne non-a, non-b hepatitis in the world, has been the subject of considerable nucleic acid sequence analysis. although all reported hepatitis c sequences from the united states have been represented by the prototype hepatitis c virus type 1 sequence, two groups of variant sequences have been reported in japan. however, we have noted five distinct, but related, genotypes (i-v) throughout the world, based on detailed sequence determination an ... | 1992 | 1323128 |
| clinical study of hepatic disorders in renal transplant recipients with special reference to hepatitis c. | 1992 | 1323155 | |
| use of aminotransferase, hepatitis c antibody, and hepatitis c polymerase chain reaction rna assays to establish the diagnosis of hepatitis c virus infection in a diagnostic virology laboratory. | clinical and therapeutic decisions for hepatitis c virus (hcv) infection depend on factors that include documentation of past infection as well as identification of those who might benefit from antiviral chemotherapy with systemic interferon. to evaluate the ability of a diagnostic laboratory to accurately identify such patients, we compared results obtained with serum transaminase assays, two hcv antibody assays (enzyme immunoassay [eia] and immunoblot), and a polymerase chain reaction (pcr)-ba ... | 1992 | 1323578 |
| detection of hepatitis c virus rna in liver needle biopsies by polymerase chain reaction. | 1992 | 1323604 | |
| low prevalence of hepatitis c virus genome detected by pcr in serum of caucasian patients with hepatocellular carcinoma. | 1992 | 1323606 | |
| [measurement of copy-number of hepatitis c virus by multicyclic rt-pcr]. | 1992 | 1323725 | |
| prospective controlled study of post-transfusion hepatitis after cardiac surgery in a large referral hospital in india. | we studied the risk of post-transfusion hepatitis (pth) in recipients of blood collected from voluntary donors screened for hbsag. two hundred and fifty patients without any previous history of liver disease or transfusion were followed up for 12 months subsequent to cardiac surgery. thirty-five of them had closed-heart surgery without receiving transfusion and served as controls. the remaining 215 patients received single-point transfusions (mean 4 +/- 2.4 units). none of the controls and 15 (6 ... | 1992 | 1323739 |
| hepatitis c virus antibody response in acute and chronic non-a, non-b hepatitis. | patients with post-transfusion, community-acquired or hemodialysis-acquired non-a, non-b hepatitis (nanbh) were tested for antibody to hepatitis c virus (hcv) during acute-phase and resolving or chronicized illness. hcv appears to be involved in most cases of post-transfusion and hemodialysis-acquired nanbh, but only in 40% of community-acquired nanbh. second generation hcv antibody assays are more specific and sensitive, favoring early detection of hcv seroconversion and identification of hcv-a ... | 1992 | 1323766 |
| behavior of antibody profile against hepatitis c virus in patients on maintenance hemodialysis. | the prevalence of anti-hepatitis c virus (hcv) in dialysis setting is still a nonstandard datum. in particular, it is not known of the phenomenon is stable or increasing or decreasing, even in a given geographical area. we studied the behavior of anti-hcv prevalence during a 12-month follow-up in 415 hemodialysis patients treated at a single institution and belonging to a limited geographical area with standard hcv endemic. point prevalence of anti-hcv has shown a tendency to growth linked in pa ... | 1992 | 1323767 |
| antibodies against hepatitis c virus in hemodialysis patients in the central italian region of umbria: evaluation of some risk factors. | the epidemiology of non-a, non-b hepatitis (nanbh) is still incomplete. to define the prevalence of antibodies against the main causative agent of nanbh, the hepatitis c virus (hcv) and the role of some risk factors, we tested sera from 269 patients on chronic dialysis at the hemodialysis units in our region in central italy. we utilized the recently developed serological assay. twenty-nine hemodialysis patients (13.3%) and 3 peritoneal dialysis patients (4.8%) were anti-hcv positive. of these, ... | 1992 | 1323768 |
| prevalence of hepatitis c virus antibodies in hemodialysis patients in the area of milan. | we studied the seroprevalence of antibodies to hepatitis c virus (hcv ab) in a cohort of 229 chronic hemodialysis patients followed by 6 dialysis units in the milan area. hcv ab was present in 51 (22.3%) of 229 examined patients. previous blood transfusions and surgery did not clearly influence hcv seroconversion, whereas duration of dialysis treatment seems to be strictly related to hcv ab positivity. | 1992 | 1323769 |
| non-a, non-b hepatitis: clinical laboratory course in patients on hemodialysis and its correlation with the presence of anti-hepatitis c virus antibodies. | 136 patients on hemodialysis, 89 males and 47 females, were studied; we evaluated the index of hepatic function (sgot and sgpt) and antibodies against hcv. we observed 42 cases of increased transaminases classified as non-a, non-b (nanb) hepatitis. antibodies against hcv were present in 40 patients. among 42 patients with nanb hepatitis. 31 (73.8%) presented anti-hcv antibodies. no significant clinical or laboratory difference exists between anti-hcv-positive and -negative patients with nanb hep ... | 1992 | 1323770 |
| antibodies to hepatitis c virus in kidney transplantation. | ninety patients on dialysis, 241 cadaveric kidney donors and 27 cadaveric kidney recipients with a follow-up of 2 years, have been investigated as for anti-hcv positivity by means of 3 tests. as for patients on dialysis and cadaveric donors, the prevalence was 32 and 4%, respectively. as for transplanted patients, it must be noted that 4 negative recipients from positive donors seroconverted, but without any change in hepatic enzymes, while in 2 or 9 anti-hcv-positive recipients, hepatic enzymes ... | 1992 | 1323771 |
| histological features of non-a, non-b hepatitis in hemodialysis patients. | in 12 hemodialysis (hd) patients with persistently raised serum alanine amino-transferase concentrations, 6 of which with anti-hcv antibodies, a liver biopsy was performed. the histological examination showed chronic persistent hepatitis or less significant changes in 11 patients and mild chronic active hepatitis in only 1. non-a, non-b hepatitis seems to demonstrate in hd patients a low tendency to induce an active and progressive liver disease. | 1992 | 1323772 |
| epidemiology of hepatitis c in a population of hemodialysis patients. | a search for antibodies against hepatitis c virus (hcv) was performed in 185 patients on chronic hemodialysis by means of 1st and 2nd generation elisa tests. immunoblot assays were performed on positive sera. this study shows a 38% prevalence of hcv-positive patients in our dialysis population according to the 2nd generation elisa test which shows a higher specificity and sensitivity when compared to the 1st generation one (38 vs. 20%). a correlation was found between the prevalence of hcv-posit ... | 1992 | 1323773 |
| hcv infection occupational hazard at dialysis units and virus spread, among relatives of dialyzed patients. | it was the purpose of this study to evaluate the spread of hcv infection among the staff at dialysis units and among relatives of anti-hcv-positive hemodialysis patients. 122 health-care workers and 52 relatives were screened for presence of anti-hcv ab. the control groups consisted of 100 health-care workers from other wards and 30 relatives of anti-hcv-negative subjects. 2.45% of the health-care staff and 5.8% of the relatives were found to be anti-hcv ab carriers. none of the subjects in the ... | 1992 | 1323774 |
| prevalence of hcv antibodies in a uraemic population undergoing maintenance dialysis therapy and in the staff members of the dialysis unit. | we studied, by both 1st and 2nd generation assay, the prevalence rate of hcvab in a population of 141 dialysis patients, 37 transplanted patients and 55 staff members. from this study emerges a higher sensitivity of the 2nd generation hcvab test (15.38 versus 36.79% of positive responses, respectively), and a significant positive correlation between lengths of dialysis period. we have not found a significant difference between hcvab-positive and -negative patients in relation to the blood transf ... | 1992 | 1323775 |
| hepatitis c virus in hemodialysis patients. | anti-hcv prevalence in 284 hemodialysis patients was assessed using the abbott hcv-eia test. anti-hcv positivity was found in 19.4% of patients, was higher in males than in females and progressively increased with age. a correlation between time of dialysis treatment, transfusions and alt course was found. these data suggest that hcv infection is a very important risk in dialysis units. | 1992 | 1323776 |
| hepatitis c virus in dialysis units: a multicenter study. | three hundred and eighty-seven chronic hemodialysis patients were evaluated, in a multicenter study, to investigate the epidemiology of hepatitis c virus. in anti-hcv seropositive patients, serum alt values and blood transfusions were retrospectively compared; blood donors were studied for serum transaminases. in seropositive patients without previous blood transfusions, analysis of dialysis schedule was done. eventually, the intrafamilial transmission of hepatitis c virus was studied in 104 fam ... | 1992 | 1323777 |
| risk factors and clinical expression of hcv infection in hemodialysis patients. | in our dialytic unit, the prevalence of antibodies to hcv (anti-hcv) was 16.4% in 1990 (73 hemodialyzed) and 21% in 1991 (105 hemodialyzed). the incidence of seroconversion was 13.1%. the transaminase behavior was similar in a group of anti-hcv-positive and a group of anti-hcv-negative patients and with previous hbv infection. blood transfusions and dialytic age did not affect anti-hcv positivity. | 1992 | 1323778 |
| prospective and retrospective assessment of clinical and laboratory parameters in maintenance hemodialysis patients with and without hcv antibodies. | in order to clear some aspects of hcv infection, we evaluated quarterly hcv markers by a riba 1 test (antigens c100-3 and 5.1.1) and monthly transaminases (alt and ast) for 14 months in 89 hbsag-free maintenance hemodialysis patients (mhp), and we retrospectively examined clinical records until the start of hemodialysis treatment. at the start of the study, 16 patients showed hcv antibodies (hcv+) and 73 were antibody-free (hcv-). 39 subjects of the staff were also examined. no hcv+ patient show ... | 1992 | 1323779 |
| hcv infections in dialysis patients. | hcv is seen as an emergent problem in dialysis units; in fact, the patients of these centers are at high risk for blood-transmitted infections, through coming microepidemies. for this reason and using the same method, we have studied the prevalence of anti-hcv and virus-derived hepatitis in a dialytic population of 105 patients. the prevalence of anti-hcv was 25.71%. | 1992 | 1323780 |
| hepatitis c virus-related acute and chronic hepatitis in hemodialysis patients. | the incidence of hcv antibodies has been evaluated in 123 chronic hemodialysis (hd) patients (group a; 55 m and 68 f) and in 37 consecutive hd patients (group b) admitted to our hospitals for acute hepatitis. in group a, hcv antibodies were present in 27% of the patients. 20 of 36 (55%) had previously received blood transfusions. 21 patients (58%) were also positive for hbv ab. in 8 patients, alt were significantly increased. in group b, the diagnosis of hcv-related acute hepatitis was made in 1 ... | 1992 | 1323781 |
| prevalence of hepatitis c infection in a hemodialysis unit. | to define the prevalence of nanb hepatitis, anti-hcv antibodies were determined in 51 patients on renal replacement therapy, in 7 transplanted patients and 17 staff members of the hemodialysis unit. anti-hcv antibodies were evaluated using immunoenzymatic methods (ortho hcv elisa test system, 1st and 2nd generation). among hemodialysis patients, seroconversion was respectively documented in 17.6% (9/51) and 52.9% (27/51); none of the transplanted patients were positive with the 1st generation te ... | 1992 | 1323782 |
| hcv infection in hemodialyzed patients: incidence and correlation with dialytic age. | hemodialyzed patients mean an high-risk population for hepatitis c infection. our work was performed to evaluate the incidence of hepatitis c virus (hcv) infection in 51 hemodialyzed patients; the presence of anti-hcv antibodies was studied using the eia and riba test of 1st and 2nd generation. 18 patients (35.29%) showed anti-hcv antibodies with the 1st test; 27 patients (52.94%) showed the presence of anti-hcv antibodies using 2nd generation test. the incidence of test positivity is not relate ... | 1992 | 1323783 |
| prevalence of antibodies against hepatitis c virus in a dialysis unit. | anti-hcv was tested in 77 uremic patients, 48 on hemodialysis (hd), 29 on capd, by immunoenzymatic 1st and 2nd generation assays (elisa i, ii) and 4-antigen (4-riba) immublotting. the investigation was extended to the staff (n = 29) and to hcv-positive patients' families (n = 30). the prevalence using 2nd generation tests was double (21%) that in 1st generation tests (11%). a greater incidence in the hd than in the capd group (23 vs. 17%) and a highly significative correlation to dialytic age we ... | 1992 | 1323784 |
| on the positivity of the test for anti-hcv considerations in dialized patients. | we have tested dialized patients with the elisa test of 1st generation for anti-hcv. all patients who resulted positive were subjected to the riba test of 1st and 2nd generation. since the positivity rate with this test is considerably more reliable, we suggest to confirm by riba test all anti-hcv-positive patients with elisa in time. | 1992 | 1323785 |
| antibodies to hepatitis c virus (anti-hcv): prevalence in the same geographical area in dialysis patients, staff members, and blood donors. | we studied the anti-hcv prevalence in the same period in all the dialysis patients, staff members and blood donors of the same geographical area. in our opinion, the low prevalence (10.26%) in dialysis patients is strictly correlated to the low prevalence (0.46%) in blood donors. | 1992 | 1323786 |
| confirmation of high prevalence of hepatitis c antibodies in hemodialysis patients by second generation immunoblot assay. | sera from 209 dialysis patients were tested for antibodies to hepatitis c virus (anti-hcv) by a 2nd generation enzyme-linked immunoassay (elisa 2) using nonstructural and core antigens. confirmation of reactivity was obtained by a 2nd generation immunoblot assay (riba 2) for antibodies to 4 separate antigens (5-1-1, c100-3, c33c, c22-3). elisa 2 was positive in 99 sera, 95 of which were confirmed by riba 2, thus accounting for an anti-hcv prevalence of 45.5%. anti-hcv positivity was correlated t ... | 1992 | 1323787 |
| high prevalence of antibodies to hepatitis c virus in hemodialysis units using a second generation assay. | we studied the prevalence and significance of antibodies to hepatitis c virus (hcv) in patients and staff from 3 dialysis units, using a 2nd generation assay (2nd g.a.; ortho hcv). of 277 patients, 151 (55%) were positive by 2nd and 85 (31%) by 1st g.a. significant associations with the anti-hcv carrier status were: blood transfusions, retrospective finding of elevated alt and duration of dialysis treatment, independently of transfusions. of the 74 staff members, 5 were positive by 2nd and 3 by ... | 1992 | 1323788 |
| comparison between first and second generation tests to determine the frequency of anti-hcv antibodies in uremic patients in replacement dialytic therapy. | research was carried out on 74 hemodialysis patients to determine the frequency of anti-hcv antibodies; this was done by means of the 1st and 2nd generation screening and control tests. the 1st generation tests showed 9 hcv-seropositive patients, while the 2nd generation tests demonstrated 15 hcv-seropositive patients. it seems evident that the 2nd generation tests are more sensitive than those of the 1st. | 1992 | 1323789 |
| anti-hcv antibody presence in hemodialyzed patients: assessment after one year regarding some preventive measures chosen. | the authors report on the presence of anti-hcv antibodies in a uremic chronic (uc) patient population after 6 months and 1 year with regard to certain selective preventive measures. the nonincreased number of hcv-positive subjects among the uc population could lead to the conclusion that the risk of environmental spreading of hcv is less than the risk of hbv diffusion. | 1992 | 1323790 |
| hcv in a group of chronic hemodialysis patients. | since hcv appears to be the major cause of post-transfusion non-a, non-b hepatitis in italy, this study determines the presence of anti-hcv in a risk group. among 26 patients, 9 were anti-hcv in a risk group. among 26 patients, 9 were anti-hcv positive with the elisa test and all of them were confirmed with the riba test of 2nd generation. only 1 had a poussés movement of alt levels. hemodialyzed patients are reactive for hcv probably for the transfusional therapy. | 1992 | 1323791 |
| anti-hepatitis c virus positivity and clinical correlations in hemodialyzed patients. | 1992 | 1323792 | |
| hepatitis c virus antibodies in dialysis pediatric patients. | a new 4-antigen recombinant immunoblot assay (4-riba) for confirmation of hepatitis c virus (hcv) c-100 enzyme-linked immunosorbent assay (elisa) reactivity was tested in serum samples of 11 pediatric patients on dialysis. of 6 hcv c-100 elisa-positive samples, all were 4-riba positive. the 2nd generation elisa picked up 1 additional case confirmed by 4-riba. the 2nd generation tests increased the prevalence of hcv-positive cases. | 1992 | 1323793 |
| comparison between first- and second-generation test for anti-hepatitis c virus antibodies in hemodialysis patients. | the serum of 387 hemodialysis patients from 9 dialysis units was checked for anti-hepatitis c virus antibodies with a 1st-generation elisa (ortho) test: 61 patients were repeatedly positive. in order to avoid false-positive results, these sera were tested with a 1st-generation confirmatory riba test, 2nd-generation screening elisa test and 2nd-generation confirmatory riba test. the 2nd-generation elisa test confirmed data obtained with 1st-generation elisa, however, the 1st-generation confirmato ... | 1992 | 1323794 |
| hepatitis c: reality of a renal unit. | hepatitis c virus (hcv) seems to be the most important agent of non-a, non-b hepatitis. this study was undertaken to assess the prevalence of hepatitis c in our renal unit. twelve patients (29%) had antibodies against hcv (anti-hcv). seropositive patients were on hemodialysis for a longer period than seronegatives. statistically significant associations with anti-hcv were: blood transfusions, at least 1 episode of elevated value of transaminases (2-fold) and fluctuations of transaminases. our fi ... | 1992 | 1323795 |
| hcv incidence in a dialysis center: preliminary reports. | incidence of seroconversion to anti-hcv from december 1989 to may 1991 was evaluated in patients and in their sexual partners. in december '89, 13 of 66 and in may '91, 26 of 75 patients were hcv positive. 9 of 13 new, seroconverted patients had been transfused. seroconverted mean dialytic age was lower than that of previous hcv-positive patients. all the sexual partners were hcv negative. blood transfusion seems to be the main risk factor for hcv infection, while environmental contamination is ... | 1992 | 1323797 |
| positive elisa 2 is pathologically relevant in elisa-1-negative patients on hemodialysis. | 1992 | 1323798 | |
| disappearance of hepatitis c virus rna in plasma during interferon alpha-2b treatment in hemophilia patients. | to establish the effect of interferon alpha-2b (ifn-alpha) treatment on hepatitis c virus (hcv) viremia, rather than monitor the alanine aminotransferase (alat) values we measured hcv-rna by cdna-polymerase chain reaction (cdna-pcr) in plasma before and during ifn-alpha treatment. eight hemophilia patients with chronic hepatitis c were treated with ifn-alpha for 24 weeks: 5 mu daily for 2 weeks, 2.5 mu daily for 4 weeks, and 1.5 mu three times a week for 18 weeks. hcv-rna, as measured by cdna-pc ... | 1992 | 1323873 |
| prevalence of markers for human immunodeficiency virus types 1 and 2, human t-lymphotropic virus type i, cytomegalovirus, and hepatitis b and c virus in multiply transfused thalassemia patients. the french study group on thalassaemia. | the prevalence of markers for human immunodeficiency virus types 1 and 2 (hiv-1, hiv-2), human t-lymphotropic virus type i (htlv-i), hepatitis b virus (hbv) and hepatitis c virus (hcv), and cytomegalovirus (cmv) was evaluated in a population of 305 multiply transfused thalassemia patients in belgium, france, and italy (sicily). no patients were found positive for hiv-2 antibodies. two french patients were seropositive for hiv-1, having been infected before systematic blood screening. antibodies ... | 1992 | 1323885 |
| improved serologic detection of hepatitis c virus with a paramagnetic microparticle assay using multiple antigenic sequences. | a paramagnetic microparticle (mp) assay for antibody to hepatitis c virus (anti-hcv) was developed, in which the probe for antibody consisted of synthetic peptides corresponding to hcv capsid and nonstructural c-100 regions, as well as a recombinant protein corresponding to the nonstructural c33c region. assay performance was evaluated by testing serum from 108 geographically diverse patients with non-a, non-b hepatitis (nanbh). the frequency of anti-hcv reactivity detected with the mp assay and ... | 1992 | 1323886 |
| detection of hepatitis c virus infection among cadaver organ donors: evidence for low transmission of disease. | to determine the prevalence of antibodies to hepatitis c virus (anti-hcv) and hcv rna among cadaver organ donors and to correlate these results with donor liver histologic abnormalities and evidence for transmission of disease through organ transplantation. | 1992 | 1323944 |
| hepatitis c virus antibodies in a long-term follow-up of beta-thalassaemic children with acute and chronic non-a non-b hepatitis. | the presence of antibodies toward hepatitis c virus (hcv) was examined in 78 polytransfused beta-thalassaemic children. the anti-hcv status was correlated with acute and chronic non-a non-b (nanb) hepatitis that developed during a follow up of about 13 years. anti-hcv was present in 83.3% of children with acute nanb hepatitis and in 82.9% of those with chronic nanb hepatitis. the percentage of chronic evolution was 56.7% for acute anti-hcv positive nanb hepatitis and 50.0% for anti-hcv negative ... | 1992 | 1324177 |
| a novel semi-automated paramagnetic microparticle based enzyme immunoassay for hepatitis c virus: its application to serologic testing. | a new rapid serologic enzyme immunoassay for antibodies to hepatitis c virus (hcv) is described. the assay combines synthetic peptide and recombinant antigens representing putative structural and non structural hcv gene products with paramagnetic microparticle assay (mp assay) technology. assay readout is based upon an enzymatically generated fluorescent product which is quantified with a novel semi-automated washer/reader instrument system. assay sensitivity and specificity was determined to be ... | 1992 | 1324258 |
| prevalence of markers of hepatotropic viruses among drug addicts in warsaw, poland. | we studied 100 unselected parenteral drug abusers for infection with hepatitis c, b, a and d virus (hcv, hbv, hav and hdv). seventy-six percent had serological evidence of hcv infection. 12% were positive for hbsag and at least one marker of hbv infection was present in 69%. these results were significantly higher than in a matched control population. compared to controls, the prevalence of anti-hav (65%) was not significantly increased in drug addicts. of the anti-hcv-positive drug addicts, 80. ... | 1992 | 1324268 |
| prevalence of markers of hepatitis viruses in out-patient alcoholics. | the prevalence of hcv, hbv and hav markers was investigated in unselected patients attending an outpatient alcoholic clinic. anti-hcv were detected in 35 (24%) of 144 patients studied, and at least one marker of hbv infection was present in 72 (50%). these results are significantly higher than in a matched control population. the presence of anti-hcv was related to previous blood transfusions and familial history of alcoholism. we conclude that alcoholics should be considered a high risk group f ... | 1992 | 1324269 |
| hepatitis c virus in kidney recipients. epidemiology and impact on renal transplantation. | in an attempt to evaluate the prevalence, kinetics and impact of hcv infection in renal transplantation, we analyzed 140 kidney recipients according to the histopathological status of the liver. thirty-three hbsag-negative patients had chronic active hepatitis, 73 hbsag-negative patients had a normal liver, 21 hbsag-negative kidney recipients had minimal pathological changes and 13 patients had hbsag-positive cirrhosis. serum antibodies to hcv were detected using the elisa from ortho diagnostic ... | 1992 | 1324270 |
| hepatitis c virus testing in primary biliary cirrhosis. | we retrospectively investigated anti-hcv prevalence in a series of 160 consecutive patients with primary biliary cirrhosis who presented between 1980 and 1989. of these, 19 (12%) were positive for anti-hcv by c-100 elisa. serum igg levels were significantly higher in anti-hcv-positive patients and correlated to optical density values. a serum sample was again collected from all the patients from the same series who were seen in 1990 for follow-up, after a median period of 32 months. anti-hcv pos ... | 1992 | 1324271 |
| cryptogenic chronic liver disease and hepatitis c virus infection in children. | the clinical features of 'cryptogenic' chronic liver disease and the prevalence of antibody to hepatitis c virus (hcv) in serum have been investigated in 33 italian children (mean age 5 years). the diagnosis was based on the persistence of increased alanineaminotransferase values for longer than 6 months after the exclusion of biliary diseases, of extra-hepatic causes of hypertransaminasemia, of infection with known hepatotropic viruses and of autoimmune or metabolic disorders. five patients had ... | 1992 | 1324275 |
| [significance of alt increase in blood donors with antiviral antibodies of hepatitis c with negative first generation elisa]. | 1992 | 1324384 | |
| [antibody prevalence for hepatitis c and other parenterally transmissible viral diseases in i. v. drug dependent patients]. | serum samples of 151 intravenous drug users were tested for markers of hepatitis b, hepatitis c, hiv and htlv-i infection to estimate the prevalence of blood born virus infection in this high risk group. anti hcv antibodies were found in 75% of sera. seroprevalence for hiv was found 13%, for hepatitis b markers 68% and none for htlv-i. multiple infections have been very frequent. | 1992 | 1324551 |
| [progress on diagnosis of hepatitis c]. | 1992 | 1324966 | |
| prevalence of hepatitis c virus rna in organ donors positive for hepatitis c antibody and in the recipients of their organs. | there is a high prevalence of liver disease among the recipients of organs from donors with antibodies to hepatitis c virus (hcv). we undertook a study to determine the frequency of persistent hcv infection, as indicated by the presence of hcv rna, among both cadaveric organ donors positive for antibodies to hcv (anti-hcv) and the recipients or organs from these donors. | 1992 | 1325035 |
| [detection of antibodies against the hepatitis c virus in cuban blood donors]. | 1992 | 1325074 | |
| hepatitis c virus and hepatocellular carcinoma. | 1992 | 1325172 | |
| prevalence of hepatitis c virus antibodies in chronic liver disease and hepatocellular carcinoma patients in india. | the prevalence of antibodies to hepatitis c virus (hcv) was investigated in 129 patients with chronic liver disease (85 with chronic active hepatitis and 44 with cirrhosis) and 53 patients with hepatocellular carcinoma. the commercially available second generation anti-hcv enzyme immunoassay kit was used. antibodies to hepatitis c virus were detected in 16.2% of the patients with chronic liver disease and in 15.1% with hepatocellular carcinoma. of the hcv positive patients in all groups 51.7% we ... | 1992 | 1325197 |
| molecular genetics of pestiviruses. | recent developments in understanding the molecular genetics of pestiviruses are reviewed. the genomic rna of several pestiviruses has been molecularly cloned and sequenced. the genetic organization of the viral protein coding region has been refined, and the complete complement of virus-encoded proteins has been elucidated. viral gene expression involves both co-translational and post-translational precursor polyprotein processing. biogenesis of the virion structural proteins requires the proteo ... | 1992 | 1325329 |
| genetic drift of hepatitis c virus during an 8.2-year infection in a chimpanzee: variability and stability. | extensive variability in genomic sequence, especially at "hypervariable regions" within the ns1/e2 region of the long open reading frame, has been reported for rna cloned from hepatitis c virus (hcv)-infected humans and chimpanzees. however, genetic changes of hcv occurring during the course of chronic infections in humans and animals have been evaluated only for partial sequences of the hcv genome. we compared two full-length cdna sequences of hcv obtained from a chimpanzee that was experimenta ... | 1992 | 1325713 |
| seropositivity to hepatitis c virus in saudi haemodialysis patients. | seropositivity to hepatitis b, c and d viruses was studied in 66 saudi haemodialysis patients from al baha region, south of saudi arabia, and was compared to that in 380 healthy saudis from the same region. the results showed that hcv is endemic in this region (3.6%) and the prevalence of anti-hcv in the haemodialysis patients was 45.5%, which is the highest reported so far in this group of patients. anti-hcv was related to the amount of blood transfused (greater than 5 units) and the duration o ... | 1992 | 1325714 |
| a study of hepatitis c virus antibodies and serum alanine amino transferase in blood donors in hong kong chinese. | the reference range of serum alanine amino transferase (alt) for the local population was established by testing 5,000 random voluntary chinese blood donors of various age groups of both sexes. in addition, 1,769 serum samples with elevated alt levels were also collected for anti-hcv assays using both the abbott and ortho anti-hepatitis c virus (hcv) assay kits. the relationship between serum alt and anti-hcv tests was studied and the performances of both kits used were compared. it was found th ... | 1992 | 1325715 |
| prevalence of hepatitis b and c and hiv antibodies in children in a romanian orphanage. | two hundred and fifteen children in an orphanage in romania were examined for serum markers of present or past hepatitis b and c virus and hiv infection. in total, 183 children (85.1%) had at least one marker (hbsag, anti-hbs or anti-hbc) of hepatitis b virus infection. an hbsag carrier state was diagnosed in 38 (20.8%) of the infected children. among the carriers 24.3% were hbeag carriers, 51.4% had anti-hbe and 24.3% had neither hbe antigen nor antibody. nine children (4.2%) had antibodies to ... | 1992 | 1325807 |
| recent developments in the molecular cloning and characterization of hepatitis c and e viruses. | the molecular properties of the genomes of both hepatitis c virus (hcv) and hepatitis e virus (hev), the major etiologic agents of non-a, non-b hepatitis, are briefly described. the organization of the genome of each of these viruses is discussed and compared to those of other related or distantly related viruses that contain single-stranded, positive-sense rna genomes. in the case of hev, the reactivity of expressed proteins and genetic divergence of geographically distinct isolates are also de ... | 1992 | 1326075 |
| hepatitis c virus infection in patients with essential mixed cryoglobulinemia. | to study the association between hepatitis c virus (hcv) infection and essential mixed cryoglobulinemia. | 1992 | 1326246 |
| non-a, non-b hepatitis and dentistry: a status report for the american journal of dentistry. | the recent identification of one of the causes of non-a, non-b hepatitis (nanbh) has led to an improved understanding of the epidemiology and possible clinical manifestations of this infectious disorder. nanbh is of some concern to dental personnel since the causative agents may be transmitted to health care workers and there is no effective immunization against nanbh. current recommendations for cross infection control in the dental workplace must apply to nanbh. | 1992 | 1326286 |
| [the evolution of non-a, non-b hepatitis: hepatitis c and hepatitis e]. | the nosological evolution of non-a, non-b hepatitis is synthetically reviewed. after the initially complete silence from the epidemiological and diagnostic points of view, much research has been devoted to this pathology and has already yielded a number of new acquisitions even beyond simple hcv and hev hepatitis. the problems connected with this pathology are a direct concern of medical practice in view of its frequency in the population and of its prevention where much progress has already bee ... | 1992 | 1326424 |
| [enzymatic amplification of sequences of viral nucleic acids. a methodological revolution promising new prospects in hepatology]. | 1992 | 1326457 | |
| [hepatitis c virus: the virus responsible of the majority of non a non b hepatitis. first part: biology of the virus and clinical and serological aspects of hepatitis c]. | 1992 | 1326461 | |
| estimation of igg subclasses of anti-hcv (c100-3) in non-a, non-b fulminant, acute and chronic hepatitis and it's significance. | to determine whether identification of subclasses of anti-hcv igg would help distinguish between acute and fulminant hepatitis, we assayed serum igg subclasses of anti-hcv (c100-3) in non-a, non-b hepatitis. anti-hcv igg was restricted to two subclasses in different diagnostic conditions; anti-hcv igg1 and anti-hcv igg3. anti-hcv igg1 was the main subclass in acute hepatitis (ah), and was positive in all the cases and only one case was positive for anti-hcv igg3. anti-hcv igg3 was the dominant s ... | 1992 | 1326465 |
| hepatitis c: virology, epidemiology, clinical course, and treatment. | 1992 | 1326784 | |
| [therapeutic difference by tae between hcc with hcv-ab and hbv-ag]. | transarterial infusion therapy using adriamycin-lipiodol emulsion (tae) was used for 30 patients of hcc with hcv-ab and 20 patients with hbv-ag. we compared the tumor effect and prognosis in terms of several clinico-pathological factors. the response rate (pr+mr) after tae was 43% in hcc patients with hcv-ab and 30% in those with hbv-ag. one-year survival rate was 89% in hcc patients with hcv-ab and 58% in hcc patients with hbv-ag. thus, there was a significant difference between the two groups. ... | 1992 | 1326926 |
| clinical and laboratory presentation of lichen planus patients with chronic liver disease. | a recent case-control study on 577 lichen planus (lp) patients and 1008 controls confirmed that lp patients may significantly associate with a chronic liver disease (cld) which is independent from drug or alcohol intake and has some connection with hepatitis b virus (hbv) infection. the study, however, failed to define the nature of cld. this has been investigated through the clinical and laboratory features of 50 patients with lp and impaired liver function tests. overall, the laboratory signs ... | 1992 | 1327093 |
| [serological results for hepatitis c in hemodialysis patients]. | 1992 | 1327172 | |
| [liver disease patients with anti-hcv. what can be done?]. | 1992 | 1327246 | |
| [characterization of hepatitis c related markers and control of hepatitis c infection]. | 1992 | 1327295 | |
| immunoglobulin preparations from hepatitis c antibody-positive plasma donors: influence on diagnosis and risk of infection in heart transplant recipients. | all heart transplant patients in our clinic received intravenous immunoglobulins as a prophylaxis against cytomegalovirus infections or reactivations. serum was sampled from 160 heart transplant patients within 4 months after surgery. in 98 samples (61%) hepatitis c virus (hcv)-specific antibodies could be detected by a "second generation" enzyme immunoassay. of these hcv antibody-positive patients 89 were tested for a second time. at this time, 5-11 months later, in 66 patients (74%) the hcv an ... | 1992 | 1327328 |
| hepatitis c virus infection in hepatocellular carcinoma. detection of plus-strand and minus-strand viral rna. | although serum antibody to hepatitis c virus (anti-hcv) is found in many patients with hepatocellular carcinoma, the actual roles of hcv in carcinogenesis are unknown. | 1992 | 1327494 |
| hcv rna and antibody to hcv core protein in japanese patients with chronic liver disease. | we evaluated the prevalence of hepatitis c virus (hcv) rna and antibody (anti-hcvcore) to the putative hcv core protein in japanese patients with chronic liver disease. sera were screened by solid-phase enzyme immunoassay with a recombinant hcv core protein and by the reverse transcription-polymerase chain reaction (rt-pcr) test which directly detects the hcv genome. anti-hcv core was detected with high titers in 95% (69/73) of chronic non-a, non-b hepatitis, and 94% (65/69) of anti-hcvcore-posi ... | 1992 | 1327684 |
| development of antibody to hepatitis c virus (hcv) in acute and chronic non-a, non-b post-transfusion hepatitis. | the antibody to hepatitis c virus (anti-hcv) was measured by an immunoassay in 507 serum samples from 94 patients with acute and chronic post-transfusion non-a, non-b hepatitis (nanb) and in 436 healthy blood donors. anti-hcv was found in 70.8 of patients with acute hepatitis, in 78.2 with chronic hepatitis, and in 1.4 of healthy blood donors. in acute hepatitis, anti-hcv appeared in the serum from 4 to 34 weeks after transfusion and from 1 to 30 weeks after the onset of the overt disease. three ... | 1992 | 1327865 |
| hepatitis c and b viruses in hepatitis b surface antigen-negative hepatocellular carcinoma. | the relative role of hepatitis c virus and hepatitis b virus in hepatitis b surface antigen-negative hepatocellular carcinoma was evaluated by polymerase chain reaction in 31 patients from taiwan. twenty-one were positive for antibody to hepatitis c virus (group 1) and 10 were negative (group 2). of the group 1 patients, hepatitis c viral rna was detected in the serum by polymerase chain reaction in 16 and in the liver tissue in 17, whereas hepatitis b viral dna was found in the liver tissue in ... | 1992 | 1327934 |
| acute hepatitis c infection: interferon finally succeeds. | 1992 | 1327936 | |
| sequence comparisons for a hepatitis c virus genome rna isolated from a patient with liver cirrhosis. | the nucleotide (nt) sequence was determined for an isolate of hepatitis c virus (hcv) derived from cirrhotic tissue of a patient with hepatocellular carcinoma. the 9408-nt sequence (embl acq. no. x61596) showed homology of 90.7-91.4% on the nt level, as compared to two japanese isolates from patients with a high titer of serum transaminase, 78.4-78.8% to those obtained in the united states, and 65.0% to that from an asymptomatic japanese carrier. the phylogenetic tree of the six isolates classif ... | 1992 | 1327977 |
| anti-hepatitis c virus antibodies in monoclonal gammopathy: an appraisal using the second generation tests. | 1992 | 1328000 | |
| treatment of children with chronic hepatitis c with recombinant interferon-alpha: a pilot study. | twelve children with chronic non-a, non-b hepatitis were entered in a pilot trial of recombinant interferon-alpha. although all the children had hepatitis c virus rna in serum, only five had antibodies against this virus. children received 3 mu/m2 body surface area interferon-alpha 3 times/wk for 6 mo; they were followed for 24 mo, including the therapy period. one child was dropped from the study, so the results are from the 11 children who completed the study. at the end of the therapy period, ... | 1992 | 1328009 |
| prevalence of antibodies to hepatitis c virus in hbsag negative hemodialysis patients. | the prevalence of hepatitis c virus (hcv) infection was estimated in a 14-month study using anti-c100-3 antibody assay in 31 hbsag negative patients on maintenance hemodialysis (mhd) for > or = 3 months. one and three patients respectively had alt elevation and anti-hcv positivity at entry. during mhd (mean period of follow up 9.9 mo), 11 (35.5%) patients had, on fortnightly estimation, alt elevation which lasted for < or = 6 months in seven patients and for > 6 months in four. fourteen (45.2%) ... | 1992 | 1328045 |
| new hepatocellular carcinoma cell line suhc-1 established from a patient with hepatitis c virus rna in serum. | a new human hepatocellular carcinoma cell (hcc) line, designated suhc-1, was derived from a japanese patient with hepatocellular carcinoma having antibody to hepatitis c virus (hcv) and hcv-rna in his serum, and established in tissue culture. this cell line exhibited typical epithelial cell morphology in culture as observed by phase-contrast and electron microscopy. the suhc-1 cells produced albumin and alpha 2-macroglobulin. chromosomal analysis showed several rearrangements at short and long a ... | 1992 | 1328123 |
| detection of replicative form of hepatitis c virus rna in peripheral blood mononuclear cells. | paired samples of plasma and peripheral blood mononuclear cells (pbmc) from 7 patients with posttransfusion hepatitis c were collected and studied for the presence of replicative forms of hepatitis c virus (hcv). rna was extracted and tested for both positive and negative strands of hcv rna by polymerase chain reaction. positive-strand rna of hcv was found in both plasma and pbmc of all 7 patients. however, negative-strand rna was found in pbmc of 3 patients while none was found in the plasma. l ... | 1992 | 1328405 |