Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| serosurvey of small carnivores in the bolivian chaco. | five species of bolivian carnivores, including nine geoffroy's cats (oncifelis geoffroyi), ten ocelots (leopardus pardalis), one jaguarundi (herpailurus yaguarondi), nine pampas foxes (pseudalopex gymnocercus), and five crab-eating foxes (cerdocyon thous) were sampled between march 2001 and april 2005 and tested for antibodies to common pathogens of domestic carnivores. carnivores were trapped in three areas: a village, the region between human settlements and a protected area, and within kaa-iy ... | 2007 | 17699100 |
| parvoviral host range and cell entry mechanisms. | parvoviruses elaborate rugged nonenveloped icosahedral capsids of approximately 260 a in diameter that comprise just 60 copies of a common core structural polypeptide. while serving as exceptionally durable shells, capable of protecting the single-stranded dna genome from environmental extremes, the capsid also undergoes sequential conformational changes that allow it to translocate the genome from its initial host cell nucleus all the way into the nucleus of its subsequent host. lacking a duple ... | 2007 | 17765706 |
| occurrence of canine parvovirus type 2c in the united states. | canine parvovirus (cpv) type 2 (cpv-2) emerged around 1978 as a major pathogen of dogs worldwide. in the mid-1980s, the original cpv-2 had evolved and was completely replaced by 2 variants, cpv-2a and cpv-2b. in 2000, a new variant of cpv (named cpv-2c) was detected in italy and now cocirculates with types 2a and 2b in that country. the cpv-2c has also been reported from single outbreaks in vietnam and spain. this study was conducted to determine if cpv-2c occurs in the united states. thirty-thr ... | 2007 | 17823398 |
| immunodetection of canine parvovirus (cpv) in clinical samples by polyclonal antisera against cpv-vp2 protein expressed in esherichia coli as an antigen. | the entire virion protein 2 (vp2) gene of canine parvovirus (cpv) was amplified by polymerase chain reaction (pcr) and engineered to be expressed by a bacterial expression vector pet-28a, under the control of the iptg-inducible t7lac promoter. sds-page gel revealed that vp2 expressed as a 67kda, and found mainly in the pellet of the bacterial lysates, suggesting that cytoplasmic expression is not preferred. the recombinant protein vp2 fused with his-tag was purified from esherichia coli using ni ... | 2007 | 17825928 |
| development and characterization of a continuous cell line, afkm-on-h, from hemocytes of the european corn borer ostrinia nubilalis (hübner) (lepidoptera, pyralidae). | the corn borer, ostrinia nubilalis, is a very important pest in different countries, and the in vitro system of the insect could be a useful tool for isolation and characterization of the pathogens and physiological responses of the insect. in this context, a cell line was derived from the hemocytes of the european corn borer and was named afkm-on-h for, respectively, o. nubilalis, armand frappier, king mongkut institutes, and hemocytes. this cell line was initiated and maintained in ex-cell 400 ... | 2007 | 17846857 |
| genetic analysis of canine parvovirus from dogs in australia. | to determine the genetic variants of canine parvovirus-2 (cpv) present in domestic dogs in australia and to investigate 26 cases of apparent vaccine failure. | 2007 | 17903125 |
| canine parvovirus types 2c and 2b circulating in north american dogs in 2006 and 2007. | parvovirus is the most common viral cause of diarrhea in young puppies. based on the analysis of a partial vp2 sequence of 54 samples, canine parvovirus type 2c (cpv-2c) (n = 26), cpv-2b (n = 25), and cpv-2 (n = 3) were detected in the united states. the american cpv-2b isolates have unique codons (494 and 572) in vp2. | 2007 | 17928423 |
| the study molecular epidemiology of canine parvovirus, europe. | canine parvovirus (cpv), which causes hemorrhagic enteritis in dogs, has 3 antigenic variants: types 2a, 2b, and 2c. molecular method assessment of the distribution of the cpv variants in europe showed that the new variant cpv-2c is widespread in europe and that the viruses are distributed in different countries. | 2007 | 17953097 |
| three-year serologic immunity against canine parvovirus type 2 and canine adenovirus type 2 in dogs vaccinated with a canine combination vaccine. | a group of client-owned dogs and a group of dogs at a commercial kennel were evaluated for duration of antibody responses against canine parvovirus type 2 (cpv-2) and canine adenovirus type 1 (cav-1) after receiving a combination vaccine containing recombinant canarypox-vectored canine distemper virus (cdv) and modified-live cpv-2, cav-2, and canine parainfluenza virus, with (c6) or without (c4) two serovars of leptospira (recombitek c4 or c6, merial). duration of antibody, which correlates with ... | 2007 | 18183549 |
| serosurvey of ex situ giant pandas (ailuropoda melanoleuca) and red pandas (ailurus fulgens) in china with implications for species conservation. | conservation strategies for the giant panda (ailuropoda melanoleuca) include the development of a self-sustaining ex situ population. this study examined the potential significance of infectious pathogens in giant pandas ex situ. serologic antibody titers against canine distemper virus (cdv), canine parvovirus (cpv), canine adenovirus (cav), canine coronavirus (ccv), canine herpesvirus, canine parainfluenza virus (cpiv), toxoplasma gondii, neospora caninum, and leptospira interrogans were measur ... | 2007 | 18229861 |
| activity of the anti-orthopoxvirus compound st-246 against vaccinia, cowpox and camelpox viruses in cell monolayers and organotypic raft cultures. | the potential use of variola virus as a biological weapon has renewed efforts in the development of antiviral agents against orthopoxviruses. st-246 [4-trifluoromethyl-n-(3,3a,4,4a,5,5a,6,6a-octahydro-1,3-di oxo-4,6-ethenocycloprop [f]isoindol-2(1 h)-yl)-benzamide] is an anti-orthopoxvirus compound active against several orthopoxviruses including vaccinia virus (vv), cowpox virus (cpv), camelpox virus (cmlv), ectromelia virus (ectv) and variola virus in cell culture. the compound has been shown ... | 2007 | 18240860 |
| sequence analysis of a canine parvovirus isolated from a red panda (ailurus fulgens) in china. | canine parvovirus (cpv) was first recognized in the late 1970 s in dogs and has mutated and spread throughout the world in canid and felid species since then. in this study, a novel cpv was isolated from the endangered red panda (ailurus fulgens) in china. nucleotide and phylogenetic analysis of the capsid protein vp2 gene classified the red panda parvovirus (rppv) as a cpv-2a type. substitution of val for gly at the conserved 300 residue in rppv presents an unusual variation in the cpv-2a amino ... | 2007 | 16927123 |
| selective regimen shift and demographic growth increase associated with the emergence of high-fitness variants of canine parvovirus. | the natural evolution of canine parvovirus (cpv) is characterized by a variety of mutations, mainly in the vp1/vp2 gene. although positive selection has been previously reported in cpv, little is known about its overall contribution to viral adaptation in the canine population. herein, the influences of the evolutive constraints on cpv during a period of viral adaptation into a previously uninfected population are more clearly investigated. to do this, 31 sequences of vp1/vp2 gene obtained from ... | 2007 | 16716762 |
| comparison of different in-house test systems to detect parvovirus in faeces of cats. | in-house tests for the identification of faecal parvovirus antigen are now available. the majority of these are licensed for canine parvovirus only; but anecdotal information suggests that they will detect feline panleukopenia virus (fpv) as well. this prospective study was designed to compare five commercially available test systems. in total, 200 faecal samples from randomly selected healthy cats (148) and cats with diarrhoea (52) were tested and compared with the results of examination by ele ... | 2008 | 18243743 |
| prognostic usefulness of blood leukocyte changes in canine parvoviral enteritis. | despite treatment, many dogs still die of complications related to canine parvoviral (cpv) enteritis. effective prognostication would be beneficial in managing this disease. | 2008 | 18371025 |
| detection of cryptosporidium parvum oocysts by dot-blotting using monoclonal antibodies to cryptosporidium parvum virus 40-kda capsid protein. | monoclonal antibodies (mab) were prepared against the 40-kda capsid protein of cryptosporidium parvum virus (cpv) by immunizing mice with purified recombinant cpv40 protein. in immunoblotting analysis, mabcpv40-1 bound to a 40-kda protein in extracts of c. parvum oocysts. this 40-kda protein was localized in the sporozoite cytoplasm by immunofluorescence (ifa) staining with mabcpv40-1. in a dot-blot assay, mabcpv40-1 was capable of detecting 10(2) non-bleach-treated and 10(2)-10(3) bleach-treate ... | 2008 | 18372626 |
| comparison of the pathogenicity in three different korean canine parvovirus 2 (cpv-2) isolates. | canine parvovirus type 2 (cpv-2) is a major pathogen inducing acute hemorrhagic gastroenteritis in dogs. despite the identification of numerous cpv-2 variants (from cpv-2a to cpv-2c), the pathogenic differences among the cpv-2 variants in dogs have not been evaluated. the aim of this study was to compare the pathogenicity of cpv-2 variants (cpv-2a-i, cpv-2a-v and cpv-2b) isolated mainly from korea. we evaluated the pathogenicity of three different cpv-2 variants, by performing clinical, hematolo ... | 2008 | 18400421 |
| towards atomic resolution structural determination by single-particle cryo-electron microscopy. | recent advances in cryo-electron microscopy and single-particle reconstruction (collectively referred to as 'cryoem') have made it possible to determine the three-dimensional (3d) structures of several macromolecular complexes at near-atomic resolution ( approximately 3.8-4.5a). these achievements were accomplished by overcoming the challenges in sample handling, instrumentation, image processing, and model building. at near-atomic resolution, many detailed structural features can be resolved, s ... | 2008 | 18403197 |
| serosurvey of pathogens in domestic dogs on the border of noël kempff mercado national park, bolivia. | the threat of disease transmission from domestic animals to wildlife has become recognized as an increasing concern within the wildlife community in recent years. domestic dogs pose a significant risk as reservoirs for infectious diseases, especially for wild canids. as part of a multifaceted ecologic study of maned wolves and other canids in the large, remote noël kempff mercado national park (nkmnp) in northeastern bolivia, 40 domestic dogs in two villages and at two smaller settlements border ... | 2008 | 18432094 |
| evidence for immunisation failure in vaccinated adult dogs infected with canine parvovirus type 2c. | an outbreak of canine parvovirus type 2c (cpv-2c) infection in vaccinated adult dogs is reported. the disease occurred in a breeding kennel in italy and affected 11 dogs aged between 6 months and 2.5 years, that had been repeatedly administered vaccines containing a type 2 (old type) cpv strain. cpv infection was demonstrated in all diseased dogs by an immunochromatographic test. a cpv strain was isolated from the intestinal content of a 20-month-old pregnant bernese mountain bitch that underwen ... | 2008 | 18437851 |
| 3.88 a structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy. | cytoplasmic polyhedrosis virus (cpv) is unique within the reoviridae family in having a turreted single-layer capsid contained within polyhedrin inclusion bodies, yet being fully capable of cell entry and endogenous rna transcription. biochemical data have shown that the amino-terminal 79 residues of the cpv turret protein (tp) is sufficient to bring cpv or engineered proteins into the polyhedrin matrix for micro-encapsulation. here we report the three-dimensional structure of cpv at 3.88 a reso ... | 2008 | 18449192 |
| [immunoadjuvant effect of the extracellular domain of canine ctla-4 on the vp2 protein of canine parvovirus]. | investigation into the adjuvant effect of the extracellular domain of canine cytotoxic t lymphocyte-associated antigen 4 (ctla-4). | 2008 | 18479065 |
| parvovirus infection in domestic companion animals. | parvovirus infects a wide variety of species. the rapid evolution, environmental resistance, high dose of viral shedding, and interspecies transmission have made some strains of parvovirus infection difficult to control within domestic animal populations. some parvoviruses in companion animals, such as canine parvovirus (cpv) 1 and feline parvovirus, have demonstrated minimal evolution over time. in contrast, cpv 2 has shown wide adaptability with rapid evolution and frequent mutations. this art ... | 2008 | 18501282 |
| assessment of serum antibody titers against canine distemper virus, canine adenovirus type ii, and canine parvovirus in alaskan sled dogs before and after a long-distance race. | to determine serum antibody titers against canine distemper virus (cdv), canine adenovirus type ii (cav-2), and canine parvovirus (cpv) in trained sled dogs prior to and after completion of a long-distance race. | 2008 | 18518808 |
| [construction, expression and immunogenicity of eukaryotic vectors based on goat pox virus p32 gene]. | the full-length p32 gene and the truncated p32 gene (mp-32) were amplified from the recombinant plasmid pmd-p32 by polymerase chain reaction (pcr) and cloned into pcdna3. 1(+) and pcdna3.1-cpg respectively. the recombinant plasmids (pcdna3.1-p32, pcdna3.1-cpg-p32 and pcdna3. 1-cpg-mp32) were transfected into bhk-21 cells by using lipofectin. the expressed p32 protein was confirmed by indirect immunofluorescence assay (ifa). the balb/c mice were immunized with these recombinant plasmids by intram ... | 2008 | 18533345 |
| canine parvovirus type 2 vaccine protects against virulent challenge with type 2c virus. | the ability of dogs vaccinated with a live attenuated cpv type 2 (nobivac intervet) vaccine to resist challenge with a current cpv2c isolate was investigated. six spf beagle dogs were given the minimum recommended course of vaccination, comprising a single inoculation of vaccine (nobivac lepto+nobivac pi) at 8-10 weeks of age followed 3 weeks later with a parvovirus vaccine in combination with distemper, adenovirus and parainfluenza virus (nobivac dhppi) and a repeat leptospirosis vaccine. six c ... | 2008 | 18006253 |
| specific identification of feline panleukopenia virus and its rapid differentiation from canine parvoviruses using minor groove binder probes. | taking into account reports of the isolation of canine parvoviruses (cpvs) from faecal samples of cats, we developed a real-time pcr assay, based on minor groove binder (mgb) probe technology, for rapid discrimination between true feline panleukopenia viruses (fplvs) from cpvs. the assay takes advantage of a single nucleotide polymorphism at position 3753 of the viral genome (corresponding to residue 323 of the capsid vp2 protein) and of the ability of mgb probes to bind specifically only to per ... | 2008 | 17850892 |
| genetic transformation of sweet orange with the coat protein gene of citrus psorosis virus and evaluation of resistance against the virus. | citrus psorosis is a serious viral disease affecting citrus trees in many countries. its causal agent is citrus psorosis virus (cpsv), the type member of genus ophiovirus. cpsv infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootstocks. certification programs have not been sufficient to control the disease and no sources of natural resistance have been found. pathogen-derived resistance (pdr) can provide an ef ... | 2008 | 17712560 |
| prevalence of antibodies against canine distemper virus and canine parvovirus among foxes and wolves from spain. | viral diseases can influence the population dynamics of wild carnivores and can have effects on carnivore conservation. hence, a serologic survey was conducted in an opportunistic sample of 137 foxes (vulpes vulpes) and 37 wolves (canis lupus) in spain for 1997-2007 to detect antibodies against canine distemper virus (cdv) and against canine parvovirus (cpv) by indirect elisa. antibodies against cdv were detected in 18.7% of the analyzed animals and antibodies against cpv in 17.2%. there was no ... | 2008 | 17646060 |
| evaluation of the antigenic relationships among canine parvovirus type 2 variants. | the antigenic relationships among the original canine parvovirus type 2 (cpv-2) and the variants cpv-2a, -2b, and -2c were evaluated. cross-antigenic evaluation revealed clear differences among the cpv variants, which were more appreciable by serum neutralization (sn) than by hemagglutination inhibition. antigenic differences were found mostly between the original cpv-2 and the variants, but they were also observed among the variants cpv-2a, -2b, and -2c. the variant cpv-2c exhibited a unique an ... | 2008 | 18160619 |
| prevalence and genetic characterization of canine parvoviruses in korea. | the prevalence of canine parvovirus (cpv) variants in dog was investigated in a total of 51 fecal samples submitted over a 2-year period (2005-2007) in korea. the cpv vp2 gene was amplified and sequenced from the fecal samples, and the results indicated that of the 51 samples, 49 samples belong to the cpv-2a family, 1 to cpv-2b, and the remaining 1 to cpv-2a variant. the vp2 gene of 20 isolates was sequenced and phylogenetic analysis was conducted. with one exception, all of the isolates were cl ... | 2008 | 18181016 |
| potential and limitation of uvc irradiation for the inactivation of pathogens in platelet concentrates. | pathogen contamination, causing transfusion-transmitted diseases, is an ongoing concern in transfusion of cellular blood products. in this explorative study, the pathogen-inactivating capacity of uvc irradiation in platelet (plt) concentrates was investigated. the dose dependencies of inactivation of several viruses and bacteria were compared with the effect on plt quality. | 2008 | 18028277 |
| sphingomyelin induces structural alteration in canine parvovirus capsid. | one of the essential steps in canine parvovirus (cpv) infection, the release from endosomal vesicles, is dominated by interactions between the virus capsid and the endosomal membranes. in this study, the effect of sphingomyelin and phosphatidyl serine on canine parvovirus capsid and on the phospholipase a(2) (pla(2)) activity of cpv vp1 unique n-terminus was analyzed. accordingly, a significant (p< or =0.05) shift of tryptophan fluorescence emission peak was detected at ph 5.5 in the presence of ... | 2008 | 18045720 |
| do two current canine parvovirus type 2 and 2b vaccines provide protection against the new type 2c variant? | three groups (n=9 or 10) of 12-week-old canine parvovirus type 2 (cpv-2) antibody-negative puppies were vaccinated: one group with a product containing modified-live cpv-2b (galaxy da2ppv; schering-plough animal health), one group with a product containing modified-live cpv-2 (continuum dap, intervet), and one group (controls) with sterile saline. all puppies receiving cpv-2 and cpv-2b vaccines developed antibody as determined by the hemagglutination inhibition assay. all groups of puppies were ... | 2008 | 18597247 |
| canine parvovirus 2c infection in central portugal. | canine parvovirus (cpv) has been evolving, generating new genetic and antigenic variants throughout the world. this study was conducted to determine the types of cpv circulating in dogs in figueira da foz, portugal. thirty fecal samples, collected between 2006 and 2007 from dogs with clinical signs of cpv infection, were tested for cpv by a rapid, in-clinic, enzyme-linked immunosorbent assay (elisa)/immunomigration test, by conventional real-time polymerase chain reaction (pcr), and by minor-gro ... | 2008 | 18599855 |
| a consensus rating method for small virus-retentive filters. ii. method evaluation. | virus filters are membrane-based devices that remove large viruses (e.g., retroviruses) and/or small viruses (e.g., parvoviruses) from products by a size exclusion mechanism. in 2002, the parenteral drug association (pda) organized the pda virus filter task force to develop a common nomenclature and a standardized test method for classifying and identifying viral-retentive filters. a test method based on bacteriophage pp7 retention was chosen based on developmental studies. the detailed final co ... | 2008 | 19055229 |
| recombination between vaccine and field strains of canine parvovirus is revealed by isolation of virus in canine and feline cell cultures. | canine parvovirus type 2 (cpv) is a pathogen that causes severe hemorrhagic gastroenteritis with a high fatality rate in pups worldwide. since cpv emerged in the late 1970s, its origin has been explored with the conclusion that cpv originated from feline panleukopenia virus or a closely related virus. both high mutation rate and recombination are assumed to be key factors in the evolution of parvoviruses. here we provide evidence for natural recombination in cpv isolated from dogs in cell cultur ... | 2008 | 19122396 |
| [new type of cytoplasmic polyhedrosis virus isolated from mosquitoes in china]. | 0507bs3 virus was isolated from a mixed pool of culex sp. and anopheles sp. collected in kashi, xinjiang, china. 0507bs3 virus could cause cytopathic effects on c6/36 cells but not on vero and bhk-21 cells. viral particles had no envelope and appeared round with diameter of about 60 nm (n = 20). viral capsid was composed of a single layer and a central core. capsomeres on the surface of capsid were clearly visible. electrophoresis of viral genome showed a profile of 10 double stranded rna (dsrna ... | 2008 | 19226951 |
| genetic analysis of vp2 gene of canine parvovirus isolates in korea. | the purpose of this study was to genetically characterize cpv isolates from korea. the vp2 gene of 31 isolates was characterized by dna sequencing and their phylogeny. among the 31 field cpv isolates, 28 isolates were classified as type 2a and other 3 isolates as type 2b. the isolates in 2a-i, ii and iii subclusters have unique mutations. the isolates in 2a-iv and v subclusters had similar amino acid sequences to type 2a isolates from other parts of the world. the isolates in type 2b had similar ... | 2008 | 18685246 |
| detecting small changes and additional peptides in the canine parvovirus capsid structure. | parvovirus capsids are assembled from multiple forms of a single protein and are quite stable structurally. however, in order to infect cells, conformational plasticity of the capsid is required and this likely involves the exposure of structures that are buried within the structural models. the presence of functional asymmetry in the otherwise icosahedral capsid has also been proposed. here we examined the protein composition of canine parvovirus capsids and evaluated their structural variation ... | 2008 | 18701590 |
| late steps of parvoviral infection induce changes in cell morphology. | previously, virus-induced non-filopodial extensions have not been encountered in connection with viral infections. here, we report emergence of long extensions protruding from norden laboratory feline kidney (nlfk) and a72 (canine fibroma) cells infected with canine parvovirus for 72 h. these extensions significantly differ in length and number from those appearing in control cells. the most striking feature in the extensions is the length, reaching up to 130 microm, almost twice the average len ... | 2008 | 18718495 |
| phylogenetic analysis reveals the emergence, evolution and dispersal of carnivore parvoviruses. | canine parvovirus (cpv), first recognized as an emerging virus of dogs in 1978, resulted from a successful cross-species transmission. cpv emerged from the endemic feline panleukopenia virus (fpv), or from a closely related parvovirus of another host. here we refine our current understanding of the evolution and population dynamics of fpv and cpv. by analysing nearly full-length viral sequences we show that the majority of substitutions distinguishing cpv from fpv are located in the capsid prote ... | 2008 | 18753238 |
| genetic analysis of feline panleukopenia viruses from cats with gastroenteritis. | thirty-nine parvovirus strains contained in faecal samples collected in italy (n=34) and uk (n=5) from cats with feline panleukopenia were characterized at the molecular level. all viruses were proven to be true feline panleukopenia virus (fplv) strains by a minor groove binder probe assay, which is able to discriminate between fplv and the closely related canine parvovirus type 2. by using sequence analysis of the vp2 gene, it was found that the fplv strains detected in italy and uk were highly ... | 2008 | 18753239 |
| the vaccinia virus fusion inhibitor proteins spi-3 (k2) and ha (a56) expressed by infected cells reduce the entry of superinfecting virus. | the orthopoxvirus spi-3 (k2) and a56 (hemagglutinin, ha) proteins interact and together prevent cell-cell fusion. spi-3/a56 has been proposed to prevent the superinfection of previously infected cells by reducing virus-cell fusion. binding of mature virions of vaccinia virus (vv) to vv-infected cells was unaffected by spi-3 or a56 on the surface of infected cells. entry of vv into infected cells was assessed using vv-p(t7)-luc carrying the luciferase reporter under t7 control. cells infected wit ... | 2008 | 18760436 |
| within-host genetic diversity of endemic and emerging parvoviruses of dogs and cats. | viral emergence can result from the adaptation of endemic pathogens to new or altered host environments, a process that is strongly influenced by the underlying sequence diversity. to determine the extent and structure of intrahost genetic diversity in a recently emerged single-stranded dna virus, we analyzed viral population structures during natural infections of animals with canine parvovirus (cpv) or its ancestor, feline panleukopenia virus (fpv). we compared infections that occurred shortly ... | 2008 | 18768982 |
| chronological analysis of canine parvovirus type 2 isolates in japan. | fifty-five canine parvovirus type 2 (cpv) samples, 12 fecal specimens and 43 cell culture isolates, were examined for their genetic characteristics of vp2 gene. they were collected from the diseased dogs at various districts of japan during 27 years from 1980 to 2006. a fragment of vp2 gene was analyzed by restriction fragment length polymorphism assay and dna sequencing. the original antigenic type 2 of cpv (cpv-2) was no longer found in the samples since 1984, and two antigenic variants cpv-2a ... | 2008 | 18772550 |
| structural evolution of reoviridae revealed by oryzavirus in acquiring the second capsid shell. | the conservation of the core structure and diversification of the external features among the turreted reoviruses appear to be relevant to structural evolution in facilitating the infection of diverse host species. the structure of rice ragged stunt virus (rrsv), in the genus oryzavirus of the family reoviridae, is determined to show a core composed of capsid shell, clamps, and long turrets. the rrsv core structure is equivalent to the core structure of orthoreovirus and the virion structure of ... | 2008 | 18787002 |
| monkeypox virus evades antiviral cd4+ and cd8+ t cell responses by suppressing cognate t cell activation. | monkeypox virus (mpv) is a virulent human pathogen that has gained increased attention because of its potential use as a bioterrorism agent and inadvertent introduction into north america in 2003. the us outbreak also provided an important opportunity to study mpv-specific t cell immunity. although mpv-specific cd4(+) and cd8(+) t cells could recognize vaccinia virus (vv)-infected monocytes and produce inflammatory cytokines such as ifngamma and tnfalpha, they were largely incapable of respondin ... | 2008 | 18796610 |
| molecular characterization of a poxvirus isolated from an american flamingo (phoeniconais ruber rubber). | an avian poxvirus from the beak scab of an american flamingo (phoeniconais ruber rubber) was isolated by inoculation on the chorioallantoic membrane (cam) of specific-pathogen-free (spf) chicken embryos. the virus produced multifocal areas of epithelial hyperplasia along with foci of inflammation in the cam, and rare cells contained small eosinophilic intracytoplasmic bodies. chickens inoculated with the isolated virus in the feather follicle of the leg did not develop significant lesions. nucle ... | 2008 | 18939646 |
| demographic effects of canine parvovirus on a free-ranging wolf population over 30 years. | we followed the course of canine parvovirus (cpv) antibody prevalence in a subpopulation of wolves (canis lupus) in northeastern minnesota from 1973, when antibodies were first detected, through 2004. annual early pup survival was reduced by 70%, and wolf population change was related to cpv antibody prevalence. in the greater minnesota population of 3,000 wolves, pup survival was reduced by 40-60%. this reduction limited the minnesota wolf population rate of increase to about 4% per year compar ... | 2008 | 18957638 |
| canine parvovirus strains identified from clinically ill dogs in the united kingdom. | 2008 | 18978368 | |
| simple tests for rapid detection of canine parvovirus antigen and canine parvovirus-specific antibodies. | canine parvovirus (cpv) is the number one viral cause of enteritis, morbidity, and mortality in 8-week-old young puppies. we have developed twin assays (slide agglutination test [sat] for cpv antigen and slide inhibition test [sit] for cpv antibody) that are sensitive, specific, cost-effective, generic for all genotypes of cpv, and provide instant results for cpv antigen detection in feces and antibody quantification in serum. we found these assays to be useful for routine applications in kennel ... | 2009 | 18987166 |
| review of companion animal viral diseases and immunoprophylaxis. | in this article we review important established, newly emergent and potential viral diseases of cats, dogs and rabbits. topics covered include virus epidemiology, disease pathogenesis, existing and prospective immunoprophylaxis against the viruses. for some feline viruses, notably the immunodeficiency virus, leukaemia virus and peritonitis virus, available vaccines are poorly efficacious but there are good prospects for this. a further challenge for the industry is likely to be due to viruses ju ... | 2009 | 19041354 |
| structural comparison of different antibodies interacting with parvovirus capsids. | the structures of canine parvovirus (cpv) and feline parvovirus (fpv) complexed with antibody fragments from eight different neutralizing monoclonal antibodies were determined by cryo-electron microscopy (cryoem) reconstruction to resolutions varying from 8.5 to 18 a. the crystal structure of one of the fab molecules and the sequence of the variable domain for each of the fab molecules have been determined. the structures of fab fragments not determined crystallographically were predicted by hom ... | 2009 | 19321620 |
| expression or subcellular targeting of virus capsid proteins with cloning genome of a canine parvovirus from china. | a strain of canine parvovirus (cpv), designated b2004, was isolated from the stool of a sick dog in beijing. the partial genome (4623bp) was cloned, sequenced with sequence showing b2004 to be a member of the widely distributed cpv-2a subclade. a completed vp2 or 11-residue n-terminal peptide (mappakrarrg) of vp1 from b2004 was also tested for its ability to mediate nuclear transport of a heterologous protein, in this case enhanced green fluorescence protein (egfp). egfp was detected in the nucl ... | 2009 | 19344923 |
| chloroplast-derived vaccine antigens and biopharmaceuticals: expression, folding, assembly and functionality. | chloroplast genetic engineering offers several advantages, including high levels of transgene expression, transgene containment via maternal inheritance, and multi-gene expression in a single transformation event. oral delivery is facilitated by hyperexpression of vaccine antigens against cholera, tetanus, anthrax, plague, or canine parvovirus (4%-31% of total soluble protein, tsp) in transgenic chloroplasts (leaves) or non-green plastids (carrots, tomato) as well as the availability of antibiot ... | 2009 | 19401820 |
| comparison of three rapid commercial canine parvovirus antigen detection tests with electron microscopy and polymerase chain reaction. | different antibody-based tests for rapid detection of canine parvovirus antigens in feces are commercially available, allowing quick diagnosis in a clinical setting. however, the diagnostic accuracy of these tests compared with standard methods has not been evaluated so far. in the current study, 3 commercial tests were compared with immune-electron microscopy (iem) and polymerase chain reaction (pcr). dogs were divided into 3 groups: group a, samples from dogs with acute hemorrhagic diarrhea (n ... | 2009 | 19407086 |
| genetic analysis of canine parvovirus type 2c. | the sequence of the full-length gene encoding for the main capsid protein vp2 of 58 canine parvovirus (cpv) type 2c strains, along with recent cpv-2a/2b strains, was determined and analysed in comparison with reference cpv isolates. the cpv-2c strains displayed a low genetic variability and shared amino acid changes already detected in recent cpv-2a/2b isolates, with a phylogenetic clustering accounting for their geographical distribution. analysis of the selection pressure driving cpv evolution ... | 2009 | 19144369 |
| strategies for improving vaccine antigens expression in transgenic plants: fusion to carrier sequences. | transgenic plants are gaining increasing attention from the industry as a natural bioreactor for the production of industrial and chemical products. optimization of transgene expression in plant cells holds the key to maximizing the potential of plants for producing proteins of commercial interest. this chapter is devoted to the description of the methods utilized for the generation of transgenic plants expressing a canine parvovirus vaccine peptide or virus-like particles from a rabbit calicivi ... | 2009 | 19183905 |
| tumor targeting using canine parvovirus nanoparticles. | advances in genetics, proteomics and cellular and molecular biology are being integrated and translated to develop effective methods for the prevention and control of cancer. one such combined effort is to create multifunctional nanodevices that will specifically recognize tumors and thus enable early diagnosis and provide targeted treatment of this disease. viral particles are being considered for this purpose since they are inherently nanostructures with well-defined geometry and uniformity, i ... | 2009 | 19198573 |
| serologic survey for canine distemper virus and canine parvovirus in free-ranging wild carnivores from portugal. | a serologic survey for canine distemper virus (cdv) and canine parvovirus (cpv) was performed on serum and lung extract from an opportunistic sample of 120 free-ranging wild carnivores (13 species) from portugal, collected from 1995 to 2006. antibodies to cdv were detected in wolf (canis lupus; 3/27) and red fox (vulpes vulpes; 2/22). antibodies to cpv were detected in wolf (9/28), red fox (2/14), wildcat (felis silvestris;1/8), genet (genetta genetta; 17/18), and stone marten (martes foina; 3/1 ... | 2009 | 19204354 |
| molecular characterization of canine parvovirus-2 variants circulating in tunisia. | canine parvovirus type 2 (cpv2) emerged in 1978 as a highly contagious and very serious disease in dogs. the characterization of cpv2 antigenic types is exclusively based on the identification of the amino acid residue at position 426 of the capsid protein vp2. currently, three antigenic types cpv-2a (asparagine n(426)), cpv-2b (aspartic acid d(426)) and cpv-2c (glutamic acid e(426)) are circulating worldwide. in tunisia, despite the fact that many clinical and few serological investigations cle ... | 2009 | 19112611 |
| parvovirus capsid disorders cholesterol-rich membranes. | in this study canine parvovirus, cpv, was found to induce disorder in dppc:cholesterol membranes in acidic conditions. this acidicity-induced fluidizing effect is suggested to originate from the n-terminus of the viral capsid protein vp1. in accordance with the model membrane studies, a fluidizing effect was seen also in the endosomal membranes during cpv infection implying an important functional role of the fluidization in the endocytic entry of the virus. | 2009 | 19118523 |
| display of neutralizing epitopes of canine parvovirus and a t-cell epitope of the fusion protein of canine distemper virus on chimeric tymovirus-like particles and its use as a vaccine candidate both against canine parvo and canine distemper. | expression of physalis mottle tymovirus coat protein in escherichia coli was earlier shown to self-assemble into empty capsids that were nearly identical to the capsids formed in vivo. amino acid substitutions were made at the n-terminus of wild-type physalis mottle virus coat protein with neutralizing epitopes of canine parvovirus containing the antigenic sites 1-2, 4 and 6-7 and t-cell epitope of the fusion protein of canine distemper virus in various combinations to yield phmv1, phmv2, phmv3, ... | 2009 | 19818723 |
| multi-immunogenic outer membrane vesicles derived from an msbb-deficient salmonella enterica serovar typhimurium mutant. | to develop low endotoxic and multi-immunogenic outer membrane vesicles (omvs), a deletion mutant of the msbb gene in salmonella enterica serovar typhimurium (s. typhimurium) was used as a source of low endotoxic omv, and an expression vector of the canine parvovirus (cpv) vp2 epitope fused to the bacterial ompa protein was constructed and transformed into the salmonella deltaamsbb mutant. in a lethality test, balb/c mice injected intraperitoneally with the salmonella deltaamsbb mutant survived f ... | 2009 | 19884791 |
| molecular insights into the phylogeny of canine parvovirus 2 (cpv-2) with emphasis on korean isolates: a bayesian approach. | a bayesian approach was utilized to further investigate the molecular phylogeny of canine parvovirus 2 (cpv-2) with emphasis on korean isolates, and the complete vp2 gene sequences for 23 korean isolates in 2007 and two vaccine strains were determined. of the 44 korean cpvs, 40 were identified as cpv-2a, three as cpv-2b, and the remaining one as a cpv-2a variant having a gly300asp mutation. compared with cpv-2a, our findings also showed the presence of six additional amino acid substitutions (th ... | 2009 | 19590812 |
| transient transfection of cryptosporidium parvum using green fluorescent protein (gfp) as a marker. | cryptosporidium parvum is a protozoan parasite that infects a variety of mammals. the parasite has been shown to harbor a dsrna virus (cpv) and in the present study, we have developed a cpv transient transfection system for this parasite by using green fluorescent protein (gfp) to replace the partial gene encoding region of the larger dsrna (cpv-l) and the smaller dsrna (cpv-s) virus. two viral rna-mediated transfection vectors: pcpvl-gfp and pcpvs-gfp were successfully constructed and both in v ... | 2009 | 19631239 |
| [secreting expression and characterization of canine parvovirus vp2 protein in eukaryotic cells]. | to study and characterize secretive expression of canine parvovirus capsid protein 2 (vp2) gene in eukaryotic cells. | 2009 | 19637574 |
| early steps in cell infection by parvoviruses: host-specific differences in cell receptor binding but similar endosomal trafficking. | canine parvovirus (cpv) and feline panleukopenia virus (fpv) are closely related parvoviruses that differ in their host ranges for cats and dogs. both viruses bind their host transferrin receptor (tfr), enter cells by clathrin-mediated endocytosis, and traffic with that receptor through endosomal pathways. infection by these viruses appears to be inefficient and slow, with low numbers of virions infecting the cell after a number of hours. species-specific binding to tfr controls viral host range ... | 2009 | 19656887 |
| desipramine induces disorder in cholesterol-rich membranes: implications for viral trafficking. | in this study, the effect of desipramine (dmi) on phospholipid bilayers and parvoviral entry was elucidated. in atomistic molecular dynamics simulations, dmi was found to introduce disorder in cholesterol-rich phospholipid bilayers. this was manifested by a decrease in the deuterium order parameter s(cd) as well as an increase in the membrane area. disordering of the membrane suggested dmi to destabilize cholesterol-rich membrane domains (rafts) in cellular conditions. to relate the raft disrupt ... | 2009 | 19741281 |
| a serological survey of infectious disease in yellowstone national park's canid community. | gray wolves (canis lupus) were reintroduced into yellowstone national park (ynp) after a >70 year absence, and as part of recovery efforts, the population has been closely monitored. in 1999 and 2005, pup survival was significantly reduced, suggestive of disease outbreaks. | 2009 | 19756151 |
| evidence for recombination between feline panleukopenia virus and canine parvovirus type 2. | canine parvovirus type 2 (cpv) is a virulent pathogen that emerged in the late 1970s, probably originating from feline panleukopenia virus (fplv) or a closely related carnivore parvovirus belonging to the feline parvovirus (fpv) subspecies. in contrast to fplv, cpv has evolved rapidly since its emergence. the original antigenic type of cpv disappeared more than two decades ago and several new antigenic as well as genetic cpv variants have appeared and spread in the field. both high mutation rate ... | 2009 | 19420841 |
| rapid detection of porcine parvovirus dna by sensitive loop-mediated isothermal amplification. | a method of loop-mediated isothermal amplification (lamp) was employed to develop a rapid and simple detection system for porcine parvovirus (ppv) dna. the amplification could be finished in 45 min under isothermal condition at 62 degrees c by employing a set of four primers targeting vp2 gene of ppv. lamp assay showed higher sensitivity than pcr, with a detection limit of 5 copies of ppv genomic dna per reaction. no cross reactivity was observed from the samples of other related viruses includi ... | 2009 | 19428576 |
| [an update on viral diseases of the dog and cat]. | in this review, recent developments in the field of viral diseases of the dog and the cat are discussed. in the dog, infection with the coronavirus type 2 is associated with respiratory signs, while infection of a highly pathogenic strain of the coronavirus type 1 has been identified as the cause of mortality in puppies. a new strain of the canine parvovirus is identified, from which the pathogenicity is not yet completely clarified. infection with west nile virus is associated with progressive ... | 2009 | 19462619 |
| detection of camel pox and vaccinia viruses by polymerase chain reaction. | pcr following two methods of dna extraction was used to confirm the growth of camel pox virus (cpv) and vaccinia virus in cell culture and chorioallantoic membrane. results were compared with the commonly used neutralization test. the first method of dna extraction was accomplished by using viral dna in tissue culture supernatant and chorioallantoic membrane, which was released by initial heating for 15 min at 99 degrees c followed by ordinary pcr. in the second method dna was extracted by using ... | 2009 | 19484375 |
| molecular characterization of canine parvovirus strains in argentina: detection of the pathogenic variant cpv2c in vaccinated dogs. | pcr amplification with sequence-specific primers was used to detect canine parvovirus (cpv) dna in 38 rectal swabs from argentine domestic dogs with symptoms compatible with parvovirus disease. twenty-seven out of 38 samples analyzed were cpv positive. the classical cpv2 strain was not detected in any of the samples, but nine samples were identified as cpv2a variant and 18 samples as cpv2b variant. further sequence analysis revealed a mutation at amino acid 426 of the vp2 gene (asp426glu), chara ... | 2009 | 19490967 |
| parvovirus induced alterations in nuclear architecture and dynamics. | the nucleus of interphase eukaryotic cell is a highly compartmentalized structure containing the three-dimensional network of chromatin and numerous proteinaceous subcompartments. dna viruses induce profound changes in the intranuclear structures of their host cells. we are applying a combination of confocal imaging including photobleaching microscopy and computational methods to analyze the modifications of nuclear architecture and dynamics in parvovirus infected cells. upon canine parvovirus i ... | 2009 | 19536327 |
| coagglutination for viral dna preparation of canine parvovirus for molecular diagnosis. | the use of pcr for molecular diagnosis is accepted as the standard method for detecting nucleic acids from numerous major infectious agents using diverse sampling techniques. although pcr is an essential tool in the research laboratory; the success of the method depends on a sample free of inhibitors that was obtained preferably by a simple and fast extraction method. the coagglutination test (coa test) is based on the propriety of protein a, from staphylococcus aureus, which can bind specifical ... | 2009 | 19539653 |
| [genetic variation analysis of canine parvovirus vp2 gene in china]. | to recognize the molecular biology character, phylogenetic relationship and the state quo prevalent of canine parvovirus (cpv), faecal samnples from pet dogs with acute enteritis in the cities of beijing, wuhan, and nanjing were collected and tested for cpv by pcr and other assay between 2006 and 2008. there was no cpv to fpv (mev) variation by pcr-rflp analysis in all samples. the complete orfs of vp2 genes were obtained by pcr from 15 clinical cpvs and 2 cpv vaccine strains. all amplicons were ... | 2009 | 20077936 |
| [biological characteristics of a chimeric rabies virus expressing canine parvovirus vp2 protein]. | to obtain a bivalence vaccine against canine rabies virus and canine parvovirus, a chimeric rabies virus expressing canine parvovirus vp2 protein was generated by the technique of reverse genetics. it was shown that the chimeric virus designated as hep-flury (vp2) grew well on bhk-21 cells and the vp2 gene could still be stably expressed after ten passages on bhk-21 cells. experiments on the mice immunized with the chimeric virus hep-flury (vp2) demonstrated that specific antibodies against rabi ... | 2009 | 19954113 |
| detection of feline panleukopenia virus using a commercial elisa for canine parvovirus. | feline panleukopenia virus (fpv) is a significant pathogen of cats. rapid virus detection is critical for treatment and management, especially in populations in which spread may occur. this study investigated the ability of the snap canine parvovirus antigen test kit (snap parvo, idexx laboratories) to detect fpv with confirmation of viral identity by polymerase chain reaction (pcr) assay and genetic sequencing on fecal samples (n = 97) from cats with suspected fpv infection. fifty-five samples ... | 2009 | 20425728 |
| oxidative stress indices in gastroenteritis in dogs with canine parvoviral infection. | gastroenteritis of viral origin has emerged as a major cause of morbidity and mortality in dogs during the last two decades. amongst the viral etiologies responsible for gastroenteritis in dogs, canine parvovirus (cpv) is considered as the most pathogenic. the disease is characterized by hemorrhagic enteritis, bloody diarrhoea and myocarditis in young pups. the present study was carried out to examine alterations in oxidative stress indices in the erythrocytes from dogs suffering from gastroente ... | 2009 | 18572211 |
| first detection of canine parvovirus type 2c in brazil. | the presence of canine parvovirus type 2 (cpv-2), 2a and 2b has been described in brazil, however, the type 2c had not been reported until now. in the current study, seven out of nine samples from dogs with diarrhea were characterized as cpv-2c, indicating that this virus is already circulating in the brazilian canine population. | 2009 | 24031389 |
| partial vp2 sequencing of canine parvovirus (cpv) strains circulating in the state of rio de janeiro, brazil: detection of the new variant cpv-2c. | canine parvovirus (cpv) is the most important enteric virus for dogs and it seems to be undergoing continuous evolution, generating new genetic and antigenic variants throughout the world. the aim of this study was to analyze the distribution of cpv variants from 1995 to 2009 and to investigate the circulation of the new variant cpv-2c in rio de janeiro, brazil. in addition, the clinical features of cpv infection were also reported. after cpv laboratorial confirmation by ha/hi and pcr, thirty-tw ... | 2010 | 24031592 |
| molecular typing of canine parvovirus occurring in pondicherry by multiplex pcr and pcr-rflp. | the present study was aimed at molecular typing of canine parvovirus (cpv) occurring in pondicherry using pcr based assays. cpv-2a and cpv-2b types were detected by pcr in 68 (53.12%) out of 128 faecal samples/rectal swabs. all the 68 samples found positive by pcr assay were subjected to multiplex pcr assay with cpv-2ab and cpv-2b primer pairs. sixty-seven (98.52%) samples were characterized as cpv-2b type and one sample (1.47%) was categorized as cpv-2a type. sixty clinical samples found negati ... | 2010 | 23637484 |
| canine parvovirus: current perspective. | canine parvovirus 2 (cpv-2) has been considered to be an important pathogen of domestic and wild canids and has spread worldwide since its emergence in 1978. it has been reported from asia, australia, new zealand, the americas and europe. two distinct parvoviruses are now known to infect dogs-the pathogenic cpv-2 and cpv-1 or the minute virus of canine (mvc). cpv-2, the causative agent of acute hemorrhagic enteritis and myocarditis in dogs, is one of the most important pathogenic viruses with hi ... | 2010 | 23637476 |
| [virus detection, clinical signs, and laboratory findings in dogs with acute hemorrhagic diarrhea: a retrospective study of 935 cases]. | the study evaluated which viruses can be detected in dogs with acute hemorrhagic diarrhea and compared signalment, clinical signs, and laboratory abnormalities among groups of dogs infected with different viruses and those that tested virus-negative. | 2010 | 22331307 |
| evaluation of the antiviral activity of chlorine dioxide and sodium hypochlorite against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. | we evaluated the antiviral activity of a chlorine dioxide gas solution (cd) and sodium hypochlorite (sh) against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. cd at concentrations ranging from 1 to 100 ppm produced potent antiviral activity, inactivating >or= 99.9% of the viruses with a 15 sec treatment for sensitization. the antiviral activity of cd was approximately 10 times higher ... | 2010 | 20616431 |
| a multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus. | a multiplex reverse transcription-nested polymerase chain reaction (rt-npcr) method was developed for the detection and differentiation of wild-type and vaccine strains of canine distemper virus (cdv). a pair of primers (p1 and p4) specific for cdv corresponding to the highly conserved region of the cdv genome were used as a common primer pair in the first-round pcr of the nested pcr. primers p2 specific for cdv wild-type strains, were used as the forward primer together with the common reverse ... | 2010 | 20433759 |
| a goat poxvirus-vectored peste-des-petits-ruminants vaccine induces long-lasting neutralization antibody to high levels in goats and sheep. | recombinant capripoxvirus (cpv) is a promising candidate differentiating infected from vaccinated animals (diva) vaccine against peste-des-petits-ruminants (ppr). in order for recombinant cpv to be successfully used in the field, there should exist dependable indicators for quality control of vaccine products, surveillance and vaccination evaluation. viral neutralization antibody (vna) is correlated to protection against ppr and is a technically feasible indicator for this purpose. the immunogen ... | 2010 | 20471441 |
| immunodeficiencies caused by infectious diseases. | immunodeficiencies caused by infectious agents may result from disruption of normal host barriers or dysregulation of cellular immunity, the latter serving to promote survival of the infectious agent through immune evasion. such infections may be followed by opportunistic infections with a variety of other microorganisms. classic infectious causes of immunodeficiency in companion animals are the immunodeficiency retroviruses, including feline immunodeficiency virus and feline leukemia virus. oth ... | 2010 | 20471525 |
| prevalence of protective antibody titers for canine distemper virus and canine parvovirus in dogs entering a florida animal shelter. | to determine the proportion of dogs entering an animal shelter with protective antibody titers (pats) for canine distemper virus (cdv) and canine parvovirus (cpv) and identify factors associated with having a pat. | 2010 | 20550446 |
| age and long-term protective immunity in dogs and cats. | vaccination can provide an immune response that is similar in duration to that following a natural infection. in general, adaptive immunity to viruses develops earliest and is highly effective. such anti-viral immune responses often result in the development of sterile immunity and the duration of immunity (doi) is often lifelong. in contrast, adaptive immunity to bacteria, fungi or parasites develops more slowly and the doi is generally short compared with most systemic viral infections. steril ... | 2010 | 19959181 |
| how baculovirus polyhedra fit square pegs into round holes to robustly package viruses. | natural protein crystals (polyhedra) armour certain viruses, allowing them to survive for years under hostile conditions. we have determined the structure of polyhedra of the baculovirus autographa californica multiple nucleopolyhedrovirus (acmnpv), revealing a highly symmetrical covalently cross-braced robust lattice, the subunits of which possess a flexible adaptor enabling this supra-molecular assembly to specifically entrap massive baculoviruses. inter-subunit chemical switches modulate the ... | 2010 | 19959989 |
| pathology in practice. enteritis. | 2010 | 20043797 | |
| isolation and characterization of feline panleukopenia virus from a diarrheic monkey. | a feline panleukopenia virus (fpv) mutant, monkey/bj-22/2008/chn, was isolated from intestinal contents of a diarrheic monkey in beijing, china. the virus was identified by morphology and physicochemical characteristics, and specific fragments were obtained by pcr using consensus primers of parvovirus and specific primers of fpv. sequence of the full-length vp2 gene of the isolated fpv was determined and analyzed by comparison with reference fpv and canine parvovirus (cpv) isolates, showing high ... | 2010 | 20044220 |
| isolation of a virus related to canine parvovirus type 2 from a raccoon (procyon lotor). | 2010 | 20045856 | |
| demography of domestic dogs in rural and urban areas of the coquimbo region of chile and implications for disease transmission. | a cross-sectional household questionnaire survey was conducted along two transects (80 and 45km long) from coquimbo and ovalle cities to the fray jorge national park (fjnp) in the coquimbo region of chile in 2005-2007 to investigate the demography of dogs in the context of a study of canine infectious diseases. data were collected on the number of dogs per household, fecundity, mortality, and sex and age distribution. the results from 1021 households indicated that dog ownership was common, with ... | 2010 | 20096943 |
| the emergence of parvoviruses of carnivores. | the emergence of canine parvovirus (cpv) represents a well-documented example highlighting the emergence of a new virus through cross-species transmission. cpv emerged in the mid-1970s as a new pathogen of dogs and has since become endemic in the global dog population. despite widespread vaccination, cpv has remained a widespread disease of dogs, and new genetic and antigenic variants have arisen and sometimes reached high frequency in certain geographic regions or throughout the world. here we ... | 2010 | 20152105 |
| binding site on the transferrin receptor for the parvovirus capsid and effects of altered affinity on cell uptake and infection. | canine parvovirus (cpv) and its relative feline panleukopenia virus (fpv) bind the transferrin receptor type 1 (tfr) to infect their host cells but show differences in the interactions with the feline and canine tfrs that determine viral host range and tissue tropism. we changed apical and protease-like domain residues by introducing point mutations and adding or removing glycosylation signals, and we then examined the interactions of those mutant tfrs with the capsids. most substitutions had li ... | 2010 | 20200243 |