Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| rapid identification of cloned hsv2 dna fragments. | randomly generated herpes simplex virus type 2 (hsv2) dna fragments can be localized on the viral genome by digestion with smai and subsequent comparison of the resulting fragment patterns with a reference picture of smai-digested recombinant plasmids that contain inserts representing the entire hsv2 (strain 333) genome as adjacent fragments. the feasibility of the technique, which we call 'sma fingerprinting' and which is much faster than fragment identification by blot hybridization, is demons ... | 1984 | 6090338 |
| use of aggregating brain cultures to study the replication of herpes simplex virus types 1 and 2 in central nervous system tissue. | a novel tissue culture system consisting of reaggregated embryonic mouse brain cells was used to examine the replication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) within central nervous system tissue. brain aggregates cultured 30-40 days in vitro demonstrated progressive maturation and differentiation into cells recognizable as neurons, astrocytes and oligodendrocytes, with the latter cell type exhibiting myelin production. mature aggregates were infected with hsv and sampled at ... | 1984 | 6099372 |
| expression of recombinant genes containing herpes simplex virus delayed-early and immediate-early regulatory regions and trans activation by herpesvirus infection. | the promoter-regulatory regions from the herpes simplex virus type 1 (hsv-1) gene for the immediate-early, 175,000-molecular-weight (175k) protein and the hsv-2 delayed-early gene for a 38k protein were linked to the readily assayable bacterial gene for the enzyme chloramphenicol acetyltransferase (cat). unexpectedly, in measurements of the constitutive expression of the recombinant genes 40 to 50 h after transfection of vero cells, enzyme levels expressed from the delayed-early 38k-promoter-cat ... | 1984 | 6092673 |
| [studies of the nature of tubular structures which appear in herpes simplex virus type 2-infected cells. studies with temperature-sensitive mutants]. | 1984 | 6099328 | |
| the immunocytochemical detection of herpes simplex virus in cervical smears--a valuable technique for routine use. | herpes simplex virus type 2 is demonstrated in routine cervical smears by immunocytochemistry. optimum antibody dilution and suitable fixation were determined and immunostaining results were found to compare favourably with those obtained by screening papanicolaou-stained smears or by virus culture methods (virus isolation). results obtained for immunostaining of smears from 158 patients agreed with those of virus isolation in 47 of 50 cases where virus isolation was performed. it is suggested t ... | 1984 | 6090625 |
| characterization of herpes simplex virus 1 alpha proteins 0, 4, and 27 with monoclonal antibodies. | analyses of the reactivity and patterns of synthesis of infected cell polypeptides (icps) specified by herpes simplex virus (hsv) 1 and 2 and by hsv-1 x hsv-2 recombinants indicated that monoclonal antibody h1183 reacted with hsv-1 alpha icp0, whereas monoclonal antibody h1113 reacted with both hsv-1 and hsv-2 alpha icp27. h1083 and h1113 and a monoclonal antibody to icp4 (h640) similar to one previously described (d. k. braun et al., j. virol. 46:103-112.) were then used to study the properties ... | 1984 | 6090689 |
| extensive homology between the herpes simplex virus type 2 glycoprotein f gene and the herpes simplex virus type 1 glycoprotein c gene. | the region of the herpes simplex virus type 2 (hsv-2) genome which maps colinearly with the hsv-1 glycoprotein c (gc) gene has been cloned, and the dna sequence of a 2.29-kilobase region has been determined. contained within this sequence is a major open reading frame of 479 amino acids. the carboxyterminal three-fourths of the derived hsv-2 protein sequence showed a high degree of sequence homology to the hsv-1 gc amino acid sequence reported by frink et al. (j. virol. 45:634-647, 1983). the am ... | 1984 | 6090692 |
| differential antiherpes activity of the (e)-and (z)-isomers of 5-(2-fluorovinyl)-2'-deoxyuridine (fvudr). | four novel analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine [(e)-brvudr]--the (e)- and (z)-isomer of 5-(2-fluorovinyl)-udr (fvudr), (z)-5-(2-carboxy-2-fluorovinyl)-udr [(z)-cooh-fvudr], and (e)-5-(2-ethoxyvinyl)-udr [(e)-eovudr] were compared with the reference compounds (e)-brvudr and 5-vinyl-udr (vudr) for their inhibitory effects on plaque formation of herpes simplex virus type 1 (hsv-1 strain 77) and type 2 (hsv-2 strain 82) in human embryonic lung fibroblast (helf) cell cultures. (z)-fvudr ... | 1984 | 6148852 |
| characterization of mrnas that map in the bglii n fragment of the herpes simplex virus type 2 genome. | the bglii n dna fragment (0.580 to 0.620 map units) of herpes simplex virus type 2 strain (333) is of interest because of its transforming potential. this fragment contains either partial or the complete coding sequences for nine mrna transcripts that can be detected during a lytic infection. subclones of the bglii n dna fragment were generated in plasmid vectors, and the approximate locations of the mrna transcripts were mapped by rna blot hybridization technology. precise 5' or 3' ends (or bot ... | 1984 | 6207312 |
| purification of herpes simplex virus-specified major dna-binding proteins and detection of antibodies to them in human and rabbit sera. | we purified herpes simplex virus type 1 and 2-specified nonstructural dna-binding proteins (icp8 and icsp 11/12, respectively) from infected vero cells and applied them in elisa test to analyse human and rabbit sera. only a weak immune response to the icp8 and icsp 11/12 could be demonstrated following experimental hsv-1 or hsv-2 infections of rabbits. low levels of icsp8 antibodies were found also in acute hsv infections of man. | 1984 | 6152122 |
| antiidiotype modulation of herpes simplex virus infection leading to increased pathogenicity. | antiidiotype reagents that recognize idiotypic determinants associated with the combining site of monoclonal antibodies to herpes simplex virus type 2 ( hsv2 ) were used to manipulate the immune response to hsv2 in balb/c mice. the injection of antiidiotype antibodies into mice before challenge with a 50% lethal dose of hsv2 resulted in a shorter survival time than that of mice receiving either preimmune rabbit immunoglobulin g or antiidiotype reagents against hepatitis b surface antigen before ... | 1984 | 6202879 |
| demonstration of a herpes simplex virus type 2-induced deoxyuridine triphosphate nucleotidohydrolase in infected kb cells and in biochemically transformed hela cells. | the deoxyuridine triphosphate nucleotidohydrolase (dutpase) activity which is induced in kb cells infected with herpes simplex virus type 2 (hsv-2) and expressed in biochemically transformed hela cells was separated from the host dutpase activity using either gel filtration or chromatofocusing chromatography. the hsv-2-induced dutpase differed from the host dutpase activity in its molecular weight (53000 versus 46000) and isoelectric point (pi 5 x 9 versus 6 x 4). however, it was similar to the ... | 1984 | 6141216 |
| the nervous system in genital herpes simplex virus type 2 infections in mice. lethal panmyelitis or nonlethal demyelinative myelitis or meningitis. | female mice were inoculated vaginally with the ms strain of herpes simplex virus type 2, and serially positive vaginal cultures were used to confirm infection. the proportion of mice infected and the mortality rate in infected mice decreased with increasing age. in mice 12 weeks old, clinical, neuropathologic, and virologic criteria defined four patterns of disease. moribund mice had severe genital lesions, hindleg paralysis, and urinary and fecal retention, and most died during the second week ... | 1984 | 6092778 |
| characterisation of a herpes simplex virus type 1 mutant which has a temperature-sensitive defect in penetration of cells and assembly of capsids. | a herpes simplex virus type 1 (hsv-1) mutant, ts1204, which has a temperature-sensitive (ts) mutation located within genome map coordinates 0.318 to 0.324, close to but outside the coding sequences of the glycoprotein gb gene, has been characterised. although this mutant adsorbed to the cell surface at the nonpermissive temperature (npt), it failed to penetrate the cell membrane. as a consequence of this defect, high multiplicities of infection of ts1204 blocked subsequent infection of cells by ... | 1984 | 6093367 |
| interactions between herpes simplex virus dna-binding proteins. | monoclonal antibodies directed against several herpes simplex virus (hsv)-induced dna-binding proteins were used to investigate protein interactions in hsv-infected cells. q1 monoclonal antibody, which is specific for the hsv-induced alkaline nuclease, when used in an immunoadsorbant column resulted in the purification of the alkaline nuclease, to which large quantities of the major dna-binding protein were bound. conversely, when a monoclonal antibody to the major dna-binding protein was used i ... | 1984 | 6094715 |
| alteration of lymphocyte transformation response to herpes simplex virus infection by acyclovir therapy. | to evaluate the effect of acyclovir (acv) therapy on the cellular immune response, we sequentially followed 43 patients with culture-proven first episodes of genital herpes simplex virus (hsv) infection. twenty-three patients who were treated with acv and 20 who received placebo had blood obtained weekly during the first 6 weeks after onset of lesions and had their in vitro lymphocyte transformation (lt) response to inactivated hsv antigens measured. the mean stimulation index to hsv antigens at ... | 1984 | 6098221 |
| herpes simplex virus types 1 and 2 homology in the region between 0.58 and 0.68 map units. | the homology between herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2, respectively) dna between 0.58 and 0.674 map units was compared by southern and dot blot analysis with dna of one type of virus as a hybridization probe against the other type. regions of high homology were interspersed with regions of detectably lower homology. however, only one region (between 0.647 and 0.653 map units) contained few or no homologous sequences. in situ rna blot hybridization demonstrated that the mrn ... | 1984 | 6092683 |
| antibodies to membrane antigens of herpes simplex virus type-2 infected cells and hsv-2 specific antibodies in patients with cervical cancer in malawi. | in a geographical study involving the united kingdom, sudan, sri lanka and malawi, sera were taken from 27 malawian patients with cervical cancer and 18 controls matched for age, ethnic origin and socioeconomic status. the sera were tested by indirect immunofluorescence for igg and iga antibodies to membrane antigens of herpes simplex virus type-2 (hsv-2). the results showed that there was no significant difference at titres 1:4 between malawian patients and controls, but at titres 1:16 the diff ... | 1984 | 6100279 |
| herpes simplex virus type-2 glycoprotein-subunit vaccine: tolerance and humoral and cellular responses in humans. | twenty-three subjects were administered three doses of inactivated herpes simplex virus (hsv) type 2 (hsv-2) glycoprotein-subunit vaccine, with doses 2 and 3 being given four and 22 weeks after dose 1. both hsv-neutralizing antibody and antibody effective in antibody-dependent cell-mediated cytotoxicity were detected in serum samples of 12 of the 13 initially seronegative subjects by week 8. the geometric mean titers of both antibody types decreased between weeks 8 and 22 but increased one week ... | 1984 | 6088648 |
| complement-requiring neutralizing antibody in guinea pigs with primary and recurrent genital herpes. | guinea pigs inoculated intravaginally with herpes simplex virus type 2 (hsv-2) strain 1868 produced a serum complement-requiring neutralizing (crn) antibody during primary acute infection, i.e., 10 days postinoculation. the crn antibody titers in the guinea pig sera decreased to less than 1:10 after heating at 56 degrees c for 30 min. it was found that 32 units of complement were necessary to obtain a satisfactory hsv-2 neutralizing antibody titer. nonheated sera significantly reduced virus infe ... | 1984 | 6091148 |
| ribonucleotide reductase of herpes simplex virus type 2 resembles that of herpes simplex virus type 1. | the ribonucleotide reductase (ribonucleoside-diphosphate reductase; ec 1.17.4.1) induced by herpes simplex virus type 2 infection of serum-starved bhk-21 cells was purified to provide a preparation practically free of both eucaryotic ribonucleotide reductase and contaminating enzymes that could significantly deplete the substrates. certain key properties of the herpes simplex virus type 2 ribonucleotide reductase were examined to define the extent to which it resembled the herpes simplex virus t ... | 1984 | 6092726 |
| herpes simplex virus genital infection of the female guinea pig as a model for the evaluation of an experimental vaccine. | substantial protection against herpes simplex type 2 (hsv 2) infection of the female guinea pig genital tract was provided by immunization with an experimental hsv 2 vaccine prepared from the plasma membranes of infected mrc-5 cells. protection was evaluated in terms of the modification of histopathological lesions and clinical signs and in changes in viral replication and serological responses in vaccinated and control animals. | 1984 | 6099646 |
| infection of murine hepatocyte cultures by herpes simplex virus (hsv) 1 and 2. | a study was undertaken of the interaction between liver cells and herpes simplex virus (hsv) in vitro. hepatocytes were obtained from hsv-resistant (c57/b16) and from hsv-susceptible (balb/c, a/j, c3h) mouse strains and cultured according to standard methods. each culture was infected with several strains of hsv-type 1 or of hsv-type 2, respectively. the multiplicity of infection was 5. the cytopathic effect was evaluated by light- and electron-microscopy. the number of infectious particles was ... | 1984 | 6094943 |
| role of cytomegalovirus in kaposi's sarcoma. | previous studies have established an association between cytomegalovirus (cmv) and classic and endemic kaposi's sarcoma (ks) which can be extended to include the epidemic form of ks. the identification of nucleic acid sequences homologous to cmv but not to epstein-barr virus (ebv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) as well as the detection of cmv gene products, particularly early antigens, in tumour biopsies and/or early cultures derived from them is an important criterio ... | 1984 | 6100282 |
| herpes simplex virus type 2 and cervical cancer. | 1984 | 6100718 | |
| herpes simplex virus latency in immunosuppressed mice. | to determine the role of antibodies in establishing herpes simplex virus (hsv) latency, immunosuppressed swiss mice were experimentally infected in the right hind footpad with a hsv-1 x hsv-2 recombinant (c6d) with low virulence. immunosuppression was induced by repeated intraperitoneal inoculations of cyclophosphamide (cy) and the production of antibodies to c6d in immunosuppressed animals was monitored by enzyme linked immunosorbent assay (elisa). within 21 days after inoculation, c6d was able ... | 1984 | 6094985 |
| rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin-streptavidin enzyme-linked immunosorbent assay. | a sensitive enzyme-linked immunosorbent capture assay with biotin and streptavidin (capture b/sa elisa) was developed to detect herpes simplex virus (hsv) antigen. rabbit anti-hsv antibody (immunoglobulin g fraction) was coated on flat-bottom, irradiated, 96-well polystyrene microtiter plates and served to capture hsv antigen. clinical specimens from patients with genital herpes were added. biotin-linked rabbit anti-hsv immunoglobulin g was used as the second antibody. the antigen-antibody compl ... | 1984 | 6086705 |
| pathogenesis of genital herpes simplex virus infection in mice. iii. comparison of the virulence of wild and mutant strains. | this study was undertaken to establish the role of virulence of various herpes simplex virus (hsv) strains in the course of infection when applying the virus to the non-injured mucous membranes of mice. wild-type hsv-type 1 (hsv-1) strains with marked differences in their neurovirulence following intracerebral inoculation showed minor differences in virulence after vaginal inoculation, but essentially their neurovirulence in cerebral infection corresponded to their virulence on the mucous membra ... | 1984 | 6096684 |
| metabolic fate of (e)-5-(2-bromovinyl)-2'-deoxyuridine in herpes simplex virus- and mock-infected cells. | (e)-5-(2-bromovinyl)-2'-deoxyuridine is a potent antiherpes compound with far better activity against herpes simplex virus type 1 than type 2. to understand the role of drug metabolism in this differential antiviral activity, we examined the metabolic fate of this drug in virus-infected and mock-infected vero cells by high-pressure liquid chromatography. after 8 h of incubation in which cells were exposed to 10 micrograms of the drug per ml, 63 pmol/10(6) cells of the parent compound was detecte ... | 1984 | 6097176 |
| antibodies to a synthetic oligopeptide that react with herpes simplex virus type 1 and 2 glycoprotein c. | nucleotide sequence and mrna localization studies have allowed the prediction of the amino acid sequence of herpes simplex virus type 1 (hsv-1) glycoprotein c (gc). we immunized a rabbit with a conjugate of bovine serum albumin and a synthetic peptide having the same sequence as that deduced for amino acids 128 through 139 of hsv-1 gc. a very similar amino acid sequence has been predicted to exist in the related product, herpes simplex virus type 2 (hsv-2) gc, which was formerly designated gf. p ... | 1984 | 6086954 |
| more rapid isolation of herpes simplex virus in a continuous line of mink lung cells than in vero or human fibroblast cells. | herpes simplex virus (hsv) was isolated from clinical specimens more rapidly in mink lung (ml) cells, a continuous cell line available from a commercial supplier, than in vero cells or human fibroblast (hf) cells. stock strains of hsv type 1 (hsv-1) and hsv-2 titered higher in ml cells than in vero or hf cells. ml cells were equivalent to rabbit kidney (rk) cells in the isolation of hsv in clinical specimens, but titers of stock hsv strains were lower. ml cells could be employed to type strains ... | 1984 | 6091988 |
| the antiviral spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine. | the antiviral activity spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) is not restricted to herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) but also encompasses several other herpesviruses such as suid herpesvirus type 1 (shv-1), bovid herpesvirus type 1 (bhv-1), simian varicella virus (svv), herpesvirus saimiri, herpesvirus platyrrhinae, and the baculovirus trichoplusia ni multiple nuclear polyhedrosis virus. other herpesviruses such as herpes simplex virus type 2, ... | 1984 | 6092320 |
| successful use of oligopeptides as immunogens in the preparation of antisera to immediate-early gene products of herpes simplex virus type 1. | antisera to two herpes simplex virus-type 1 (hsv-1) immediate-early gene products ( ie12 and ie175 ) have been produced using oligopeptides, constructed on the basis of proposed dna coding sequences, as immunogens. in both cases the synthetic peptides were linked to bovine serum albumin via an n-terminal tyrosine prior to immunization. both the ie12 and the ie175 antisera reacted with their respective hsv-1 antigens and with antigens produced by the hsv-1 immediate-early mutant tsk but not with ... | 1984 | 6202828 |
| human cytotoxic t cell clones directed against herpes simplex virus-infected cells. ii. bifunctional clones with cytotoxic and virus-induced proliferative activities exhibit herpes simplex virus type 1 and 2 specific or type common reactivities. | human cytotoxic t lymphocyte (ctl) clones directed against herpes simplex virus (hsv)-infected cells were generated after stimulation of peripheral blood lymphocytes (pbl) with hsv type 1 (hsv-1) and hsv type 2 (hsv-2). these ctl clones were studied with regard to hsv type specificity and with regard to whether they also express helper cell activity. some clones, generated after stimulation with hsv-1, were cytotoxic for autologous cells infected with either hsv-1 or hsv-2 ("hsv type common clon ... | 1984 | 6207243 |
| localization of a type-specific antigenic site on herpes simplex virus type 2 glycoprotein d. | a herpes simplex virus type 1 strain isolated from a recurrent lesion of the nose reacted with monoclonal antibodies recognizing a type 2-specific site on glycoprotein d but not with monoclonal antibodies recognizing other type 2-specific sites. dna sequence analysis of the glycoprotein d gene of the isolate revealed a single nucleotide alteration which changed the codon for asparagine to one encoding histidine at amino acid 97 in the protein. histidine is located at this position in glycoprotei ... | 1984 | 6202883 |
| a rapid procedure for the enrichment of undenaturated, antigenically active herpes simplex virus glycoproteins. | the usefulness of lentil lectin affinity chromatography for the rapid enrichment of hsv glycoproteins in an undenatured state for both research and clinical purposes was investigated. in order to compare the lentil lectin-binding characteristics and immunologic specificities of undenatured hsv-1 and hsv-2 glycoproteins, [35s]methionine-labelled extracts of virus-infected hep-2 cells were subjected to lentil lectin affinity chromatography. individual hsv-1 and hsv-2 glycoproteins in bound and unb ... | 1984 | 6200489 |
| fine structure analysis of type-specific and type-common antigenic sites of herpes simplex virus glycoprotein d. | the fine structure of the antigenic determinants of herpes simplex virus type 1 and 2 glycoprotein d (gd) was analyzed to determine whether structural differences underlie the differential immunogenicity of these glycoproteins. a region common to herpes simplex virus type 1 and 2 gd (amino acid residues 11 to 19) and two sites specific for herpes simplex virus type 2 gd (one determined by proline at position 7, the other determined by asparagine at position 21) were localized within the n-termin ... | 1984 | 6208376 |
| potent synergistic inhibition of herpes simplex virus-2 by 9-[(1, 3-dihydroxy-2-propoxy)methyl]guanine in combination with recombinant interferons. | dhpg, an acyclic guanine nucleoside with the structure 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine], showed potent synergism with recombinant alpha or beta interferons and modest synergism with gamma interferon in inhibiting the replication of herpes simplex virus type 2 in vitro. the most potent direct anti-herpes viral synergism was obtained by combination of dhpg and recombinant human interferon-beta-ser17; when combined, doses of each near their separate effective dose50's resulted in almost ... | 1984 | 6201172 |
| sensitivity and specificity of monoclonal antibodies directed against antigenic determinants of treponema pallidum nichols in the diagnosis of syphilis. | murine anti-treponema pallidum monoclonal antibodies were employed in studies on sensitivity and specificity of binding to examine their potential for use in the detection of low numbers of pathogenic treponemes present in various body fluids. monoclonal antibodies were used as a primary antibody source in a solid-phase immunoblot assay system. all monoclonal antibodies assayed were capable of detecting ca. 1.0 x 10(3) to 2.5 x 10(3) treponemes. of 13 monoclonal antibodies examined, 3 were able ... | 1984 | 6208218 |
| identification and preliminary mapping with monoclonal antibodies of a herpes simplex virus 2 glycoprotein lacking a known type 1 counterpart. | the properties of herpes simplex virus 2 (hsv-2)-specific proteins reactive with monoclonal antibody h966 derived from mice immunized with hsv-2 strain g are reported. the reactive proteins contained in infected cell lysates subjected to electrophoresis in denaturing gels and transferred to nitrocellulose sheets form a relatively sharp band characteristic of mr 124,000 proteins and a diffuse, more slowly migrating band. antigens reactive with h966 were detected on the surface of viable, unfixed ... | 1984 | 6199891 |
| treatment of primary acute genital herpes in guinea pigs by intraperitoneal administration of fluoropyrimidines. | fiac [1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine], fiau [1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodouracil], and fmau [1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-methyluracil] were evaluated for their efficacies in the treatment of genital infections with herpes simplex virus type 2 in guinea pigs. intraperitoneal administration of these drugs in daily doses of 100 mg/kg of body weight initiated 24 h after virus inoculation and repeated 2 successive days therea ... | 1984 | 6239591 |
| inhibition of the herpes simplex virus-coded thymidine kinase-complex by 9-beta-d-arabinofuranosyladenine 5'-monophosphate (ara-amp) and 9-(2-hydroxyethoxymethyl)guanine-monophosphate (acyclo-gmp). | the thymidine kinase-complex isolated from herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) is associated with the following enzyme activities:atp:dthd (dcyd) deoxypyrimidine kinase, atp:dtmp thymidylate kinase, adp:dthd- and amp:dthd 5'-phosphotransferase. in kinetic experiments it is shown that ara-amp inhibits amp:dthd- and adp:dthd phosphotransferase activity, while acyclo-gmp impairs adp:dthd phosphotransferase reaction only; the inhibition was found to be non-competitive. the funct ... | 1984 | 6206825 |
| clonal analysis of t-cell responses to herpes simplex virus: isolation, characterization and antiviral properties of an antigen-specific helper t-cell clone. | a herpes simplex virus (hsv)-specific long-term t-cell clone has been established from the draining lymph node cells of balb/c mice; the cells required repeated in vitro restimulation with uv-irradiated virus. the established t-cell clone expresses the thy-1 and lyt-1+2,3- surface antigens. for optimal proliferation of the cloned cells, both the presence of specific antigen and an exogenous source of t-cell growth factor are required. the proliferative response of the cloned t cells was found to ... | 1984 | 6209206 |
| localization and synthesis of an antigenic determinant of herpes simplex virus glycoprotein d that stimulates the production of neutralizing antibody. | an antigenic determinant capable of inducing type-common herpes simplex virus (hsv)-neutralizing antibodies has been located on glycoprotein d (gd) of hsv type 1 (hsv-1). a peptide of 16 amino acids corresponding to residues 8 to 23 of the mature glycoprotein (residues 33 to 48 of the predicted gd-1 sequence) was synthesized. this peptide reacted with an anti-gd monoclonal antibody (group vii) previously shown to neutralize the infectivity of hsv-1 and hsv-2. the peptide was also recognized by p ... | 1984 | 6197535 |
| immunofluorescent evidence of prior herpes simplex virus type-2 infection in prostate carcinoma. | the finding of herpes simplex virus type-2 (hsv-2) particles in prostatic carcinoma (pca) tissue has led to speculation that the virus might cause this disease. we studied 27 pca and 33 benign prostatic hyperplasia (bph) specimens for the presence of hsv-2 antigens by indirect immunofluorescent staining to hsv-2 using commercially prepared rabbit anti-hsv-2 and fluorescein-tagged goat and anti-rabbit antibody. the slides were randomly number coded by an impartial referee then read independently ... | 1984 | 6209848 |
| [detection of the virus-specific antigens of adenoviruses and herpes simplex virus in patients with malignant genital diseases]. | the possibility of detecting virus-specific antigens in women with various pathological changes of the genitalia (cancer and pre-cancer conditions) was demonstrated. comparative examinations of specimens from patients with cancer diseases of the genitalia for the presence of structural and virus-induced early antigens of hsv-2 demonstrated immunofluorescence test (ift) to be the diagnostic test for the detection of hsv antigens in patients with cervical carcinoma. the rate of virus-induced antig ... | 1984 | 6084901 |
| herpes virus antigens and antibodies in kidney biopsies and sera of iga glomerulonephritic patients. | antibody titers against 4 viruses of the herpes group have been studied in the sera of 54 patients with iga glomerulonephritis (gn). the incidence of antibodies to herpes simplex virus type-1 (hsv-1), hsv-2, cytomegalovirus (cmv), epstein-barr virus nuclear antigen (ebna), and that of igg antibodies to ebv virus capsid antigen (vca) were similar in patients and in controls. however, elevated antibody titers (greater than or equal to 64) to hsv and cmv occurred more frequently in patients with ig ... | 1984 | 6329570 |
| expression of a herpes simplex virus glycoprotein in two clonal lines of hamster cells transformed by herpes simplex virus type 2. | expression of herpes simplex virus type 2 (hsv-2) glycoproteins on the cell surface of two clones of a hamster cell line transformed by hsv-2 was examined by immunofluorescence tests with hamster antisera prepared against three size-class glycoprotein fractions obtained from hamster embryo fibroblast cells infected with hsv-2. enhanced expression of one of the three size-class glycoprotein fractions (molecular weight, 82,000-94,000) was found in one clone (155-4-213) after replating of the cells ... | 1984 | 6329882 |
| genital herpes simplex infections: effect of asymptomatic shedding and latency on management of infections in pregnant women and neonates. | asymptomatic shedding of herpes simplex virus (hsv) at delivery occurred in 2.4% of women with a past history of recurrent genital infections; using current methods, this could not be predicted in advance. in addition, only 22% of the mothers of infected infants had an elicitable history of recurrent genital infections. passively derived neutralizing antibody titers of 1:106 to hsv-1 and 1:67 to hsv-2 were found in 22 exposed infants who remained asymptomatic as compared with 1:8 and 1:8, respec ... | 1984 | 6330222 |
| herpes simplex virus type 2 establishes latency in the mouse footpad and in the sensory ganglia. | three mouse strains as well as wt and 7 ts mutants of herpes simplex virus (hsv) type 2 (strain hg52) have been used to investigate latency. the mice were inoculated in the right rear footpad. virus reactivation following explanation and culture of the dorsal root ganglia and the footpad was scored. the results show that: hsv-2 can be maintained in the mouse footpad in a state indistinguishable from latency; virus gene functions necessary for latency can be identified by the use of ts mutants; a ... | 1984 | 6330225 |
| hsv, cmv, and hpv in human neoplasia. | we are studying the role of sexually transmitted viruses in the development of human tumors. the persistence of herpes simplex virus, cytomegalovirus, and human papillomavirus nucleic acid sequences has been examined using cloned viral dna sequences as probes. the relationship of the viruses to various stages in the progression of neoplasia is examined, with particular reference to the role of viral and/or cellular genes in the initiation, promotion, and maintenance of the neoplastic phenotype. ... | 1984 | 6330227 |
| a rapid method for restriction endonuclease analysis of dna from herpes simplex virus type 2. | 1984 | 6330274 | |
| evaluation of the effectiveness of 2h-1,3-oxazine-2,6(3h)-dione (oxauracil) in the treatment of herpes simplex virus (hsv)-induced acute encephalitis in mice. | treatment of suckling mice with the pyrimidine analogue 2h-1,3-oxazine-2,6(3h)-dione (oxauracil) proved successful in reducing mortality associated with hsv-2-induced encephalitis. oxauracil did not decrease mortality associated with hsv-1 infection; however, there was an increased survival time and a decrease in the amount of infectious virus recovered from the brains of hsv-1-infected animals. | 1984 | 6330291 |
| nucleotide sequence of the marmoset herpesvirus thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide. | the nucleotide sequence of a 2549-bp dna fragment containing the entire coding region of the marmoset herpesvirus (marhv) thymidine kinase gene (tk) and the flanking sequences was determined by the dideoxynucleotide chain termination method. the marhv thymidine kinase polypeptide predicted from the nucleotide sequence contained 376 amino acids and had a molecular weight of 41,281. the sequencing data also reveal that the coding portion of another marhv gene probably begins only 292 nucleotides d ... | 1984 | 6330976 |
| enhanced efficacy of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine in combination with alpha-interferon against herpes simplex virus type 2 in mice. | the acyclic nucleoside dhpg [9-(1,3-dihydroxy-2-propoxymethyl)guanine] and recombinant human alpha-interferon of clones a/d potentiate each other's antiviral activity against a systemic infection with herpes simplex virus type 2. the effective dose at which 50% of the mice survived was lowered approximately 10-fold for dhpg when it was given in combination with a marginally effective dose of alpha-interferon and greater than 10-fold for alpha-interferon when it was given in combination with a no ... | 1984 | 6335382 |
| thymine arabinoside (ara-t) topical and iontophoretic application for herpes simplex virus type 1 and type 2 skin infections in hairless mice. | the antiviral agent thymine arabinoside (9-beta-d-arabinofuranosyl thymine, ara-t) was applied topically and by anodal (+) iontophoresis in a nacl solution to herpes simplex virus type 1 (hsv-1), and herpes simplex virus type 2 (hsv-2) skin infections in hairless mice. the chemotherapeutic efficacy of ara-t was evaluated employing the parameters of mean lesion score, number of mice with signs of neurological involvement (paralysis), mortality and mean survival time. anodal (+) iontophoresis of 0 ... | 1984 | 6717166 |
| human monocytes activated by immunomodulators in liposomes lyse herpesvirus-infected but not normal cells. | highly purified peripheral blood monocytes from normal human donors were activated in vitro by incubation with liposomes containing immunomodulators such as recombinant human gamma interferon, human lymphokines, or muramyl dipeptide. the ability of liposomes containing immunomodulators to activate monocytes to a cytotoxic state capable of discriminating between virus-infected and uninfected cells was shown by activated monocytes recognizing and destroying herpes simplex virus type 2-infected cel ... | 1984 | 6426057 |
| immunotoxic effects of diethylstilbestrol on host resistance: comparison with cyclophosphamide. | to evaluate the usefulness of host resistance assays for measurement of immunotoxicologic effects of chemicals, the immunosuppressive effects of exposure to diethylstilbestrol (des) were compared with the effects of treatment with the known immunosuppressive drug cyclophosphamide (cps). a panel of six host resistance models was evaluated, including infection with the bacterium listeria monocytogenes, herpes simplex virus type 2 (hsv-2), and encephalomyocarditis virus (emc), the yeast cryptococcu ... | 1984 | 6323603 |
| characterization of the 92,000-dalton glycoprotein induced by herpes simplex virus type 2. | evidence is presented showing that the 92,000-dalton glycoprotein (g92k) induced by herpes simplex virus (hsv) type 2 has properties distinct from those assigned to any other hsv glycoprotein. first, the carbohydrate composition and extent of sulfation differ from those of glycoproteins d and e. second, two clonally unrelated monoclonal antibodies, ap1 and lp5, shown in this paper to specifically immunoprecipitate g92k, do not react with any of the known processed forms of glycoproteins b, c, d, ... | 1984 | 6323754 |
| resistance of herpes simplex virus to 9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine: physical mapping of drug synergism within the viral dna polymerase locus. | a herpes simplex virus type 2 (hsv-2) mutant ts6 (strain hg52) induces a heat-labile viral dna polymerase at the nonpermissive temperature and is markedly resistant to 9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]-guanine [2'-nor-2'-deoxyguanosine; 2'ndg]. this antiviral drug requires hsv thymidine kinase for phosphorylation to an active inhibitor (2'ndg-triphosphate), and thymidine kinase-deficient mutants of hsv exhibit varying degrees of resistance to 2'ndg, with the hsv type 1 (hsv-1) b2006 ... | 1984 | 6324203 |
| herpes simplex virus infection causes the accumulation of a heat-shock protein. | a monoclonal antibody, produced from mice immunized with a herpes simplex virus (hsv)-infected cell extract, reacts with a molecule which is present in uninfected cells and which accumulates in large amounts during hsv 2 infection. in uninfected cells this molecule is growth regulated, in that exponentially growing cells have intense nuclear immunofluorescence, whereas confluent quiescent cells have little. it has a mol. wt. of 57 000 (p57) in exponential cells, and one of 61 000 (p61) in quiesc ... | 1984 | 6325164 |
| the presence of rna complementary to hsv-2 (herpes simplex virus) dna in cervical intraepithelial neoplasia after laser therapy. | in-situ hybridization of radiolabelled dna probes to tissue sections detected rna complementary to hsv-2 (herpes simplex virus) dna in 65% (22 of 34) biopsies from cervical intraepithelial neoplasia (cin) and in none of internally paired benign epithelia from individuals before treatment by laser vaporization cone. laser therapy did not produce an increase in clinical or subclinical hsv infections of the cervix. of 18 patients with rna complementary to hsv-2 dna in cin biopsies before treatment, ... | 1984 | 6322834 |
| calcium ionophore a-23187 and 12-o-tetradecanoyl-phorbol-13-acetate stimulation of prostaglandin synthesis in herpes simplex virus type 2-transformed rat cells. | the purpose of this investigation was to determine whether cells transformed by herpes simplex virus type 2 (hsv-2) can be stimulated to synthesize prostaglandins (pg). stimulation was determined by measuring the release of pg into overlay fluids from cell monolayers prelabeled with [3h]arachidonic acid. results showed that ca2+ ionophore a-23187 markedly stimulated arachidonic acid release starting 30 min after treatment of hsv-2-transformed and nontransformed rat embryo fibroblast cells. howev ... | 1984 | 6322982 |
| replication origins and a sequence involved in coordinate induction of the immediate-early gene family are conserved in an intergenic region of herpes simplex virus. | we have determined the structure of the 5' portion of herpes simplex virus type 2 (hsf-2) immediate-early (ie) mrna-3 and have obtained the dna sequence specifying the n terminus of its encoded polypeptide, vmw182, its untranslated leader and the intergenic region between iemrnas-3 & 4/5. comparison of the hsv-2 intergenic sequences with the hsv-1 equivalent region identifies several conserved regions: (1) an at-rich element with core consensus taatgarat which is likely to be the 'activator' seq ... | 1984 | 6322134 |
| the junctions between the repetitive and the short unique sequences of the herpes simplex virus genome are determined by the polypeptide-coding regions of two spliced immediate-early mrnas. | immediate-early (ie) mrnas-4 and -5 of herpes simplex virus type 2 (hsv-2) are transcribed from the irs/trs genome regions towards the us region. each of these spliced mrnas has an untranslated leader sequence of 249 bases and a single intron of approximately 540 bases which are contained entirely within trs/irs sequences. the dna sequence of the intron largely comprises tandem reiterations of three distinct short sequences. upstream of the common 5' mrna termini the dna sequence contains region ... | 1984 | 6321634 |
| differential substrate specificity of dna polymerase beta and of a dna polymerase induced by herpes simplex virus type 2 towards thymidine triphosphate analogues. | several triphosphates of 5-substituted deoxyuridine (du), such as 5-ethyl-, 5-n-propyl-, 5-n-hexyl- and 5-isopropyldeoxyuridine triphosphates and 5-trifluorothymidine triphosphate are substrates for hela cell dna polymerase beta (2'-deoxynucleoside-5'-triphosphate:dna-deoxynucleotidyltransferase, ec 2.7.7.7) and for a dna polymerase isolated from hela cells infected with herpes simplex virus type 2 (hsv-2) strain 75. at the concentration tested (50 microm), all these analogues were incorporated ... | 1984 | 6321635 |
| determination of herpes simplex virus type-specific antibodies by solid-phase ria on helix pomatia lectin-purified antigens. | herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) antibodies in humans and animals were determined by a micro-solid-phase radioimmunoassay with helix pomatia lectin/sepharose 4b-purified antigens from hsv-1- and hsv-2-infected cells. a low but constant heterotypic reactivity was found with hsv-1 antigen, whereas hsv-2 antigen was type specific, as evidenced by (1) its reactivity with sera from hsv-2- but not with those from hsv-1-immunized animals, (2) its reactivity with monoclonal antibod ... | 1984 | 6330240 |
| intra-axonal virus in demyelinative lesions of experimental herpes simplex type 2 infection. | three-week-old mice which had been infected intracerebrally with herpes simplex virus type 2 (hsv-2) were examined electron-microscopically for the presence of intra-axonal virus in or near optic nerve and spinal cord demyelinative lesions. acute lesions and their margins frequently contained a very small proportion of abnormal axons, and in a few of these mature virus particles, nucleocapsids, or other incomplete forms were found. a similar range of particle morphology was present in the cytopl ... | 1984 | 6321668 |
| synthesis of (e)-5-(3,3,3-trifluoro-1-propenyl)-2'-deoxyuridine and related analogues: potent and unusually selective antiviral activity of (e)-5-(3,3,3-trifluoro-1-propenyl)-2'-deoxyuridine against herpes simplex virus type 1. | syntheses of (e)-5-(3,3,3-trifluoro-1-propenyl)-2'-deoxyuridine (tfpe-durd) (1), 5-(3,3,3-trifluoro-1-propyl)-2'-deoxyuridine (11), 5-(3,3,3-trifluoro-1-methoxy-1-propyl)-2'-deoxyuridine (8), and 5-(3,3,3-trifluoro-1-hydroxy-1-propyl)-2'-deoxyuridine (10) from 5-chloromercuri-2'-deoxyuridine are described. the antiviral activity of tfpe-durd was determined in cell culture against herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), and vaccinia virus and compared concurrentl ... | 1984 | 6321735 |
| homology between two ebv early genes and hsv ribonucleotide reductase and 38k genes. | computer-matching of amino acid sequences predicted from the complete ebv dna sequence against the known hsv gene sequences has revealed significant homology between two ebv reading frames and the hsv1 and hsv2 140k and 38k proteins which are associated with ribonucleotide reductase activity. the two genes are arranged tandemly as in hsv though it appears that, unlike hsv, the two mrnas are not 3' co-terminal. we have mapped two promoters predicted from the dna sequence for these genes and shown ... | 1984 | 6330697 |
| organization of the left-hand end of the herpes simplex virus type 2 bglii n fragment. | we have determined the complete nucleotide sequence surrounding the region coding for a 38,000-dalton protein of herpes simplex virus type 2 strain 333 that is encoded between map coordinates 0.58 to 0.595 on the viral genome. the sequence data have revealed an open translational reading frame of 1,011 nucleotides encoding a protein of 337 amino acids. upstream of those sequences, transcriptional regulatory signals could be identified that overlap another open reading frame of 489 nucleotides, w ... | 1984 | 6321759 |
| limited proteolysis of herpes simplex virus glycoproteins that occurs during their extraction from vero cells. | herpes simplex virus glycoproteins extracted from infected vero cells can be smaller in apparent size than the same viral products extracted from infected hep-2 cells. here we show that the differences in size result primarily from limited proteolysis, during or after their extraction, of the viral glycoproteins made in vero cells. in the absence of appropriate protease inhibitors, both mature and immature forms of four different glycoproteins specified by herpes simplex virus type 2 were signif ... | 1984 | 6321794 |
| dna polymerase in nuclei isolated from herpes simplex virus type-2-infected cells. characterization of the reaction product and inhibition by substrate analogs. | nuclei isolated from herpes simplex virus (hsv) type 2-infected kb cells were examined for their capacity to serve as an in situ source of herpes dna polymerase. in contrast to purified enzymes with added template, approx. 80% of the dna synthesized in isolated nuclei was viral. the average size of dna fragments labeled in vitro was 3.2 x 10(6) da. based on an increase in dna density when nuclei were incubated in the presence of brdutp rather than dttp, 16% of the nucleotides were added during t ... | 1984 | 6320890 |
| structural organization of human herpesvirus dna molecules. | the herpesviruses are among the largest and most complex of all dna viruses, and their genomes display an astonishing diversity in size, structure, and organization. in 1974, the features of large inverted repeats and structural isomerization were first discovered, and these proved to be characteristic properties of many herpesvirus genomes. since then, research using the powerful techniques of modern molecular biology has revealed a great deal of comparative structural information about the arr ... | 1984 | 6330219 |
| glycoprotein c of herpes simplex virus 1 acts as a receptor for the c3b complement component on infected cells. | receptors for the fc portion of immunoglobulins or for the third component of complement (c3) are present on a variety of circulating and fixed tissue cells including granulocytes, monocytes, lymphocytes and glomerular epithelial cells. cells which lack fc receptors may express them after infection by herpes simplex virus (hsv)-1, hsv-2, cytomegalovirus or varicella zoster virus. we recently reported that infection by hsv-1 induces both fc and c3 receptors on human endothelial cells. glycoprotei ... | 1984 | 6328323 |
| prospective study on the relationship between cervical neoplasia and herpes simplex type-2 virus. ii. herpes simplex type-2 antibody presence in sera taken at enrollment. | sera obtained at enrollment in the study from patients suffering from moderate to sever dysplasia (cervical intraepithelial neoplasia grade ii), carcinoma in situ (cervical intraepithelial neoplasia grade iii) and invasive carcinoma, or developing any of these conditions in the course of the prospective study, and from control subjects, were examined for herpes simplex type-2 (hsv-2) antibody presence. the controls were matched with the patients by age, age at first intercourse, number of sexual ... | 1984 | 6319297 |
| phosphorylation of four acyclic guanosine analogs by herpes simplex virus type 2 thymidine kinase. | four acyclic guanosine analogs, the (r) and (s) enantiomers of 9-(3,4-dihydroxybutyl)guanine, 9-(4-hydroxybutyl)guanine, and acyclovir were compared in enzyme kinetic experiments, using purified herpes simplex virus type 2 thymidine kinase. all four analogs showed competitive patterns of inhibition in the phosphorylation of thymidine by the viral thymidine kinase, but different affinities and relative rates of phosphorylation were observed. | 1984 | 6329085 |
| amino-terminal sequence of glycoprotein d of herpes simplex virus types 1 and 2. | glycoprotein d (gd) of herpes simplex virus is a structural component of the virion envelope which stimulates production of high titers of herpes simplex virus type-common neutralizing antibody. we carried out automated n-terminal amino acid sequencing studies on radiolabeled preparations of gd-1 (gd of herpes simplex virus type 1) and gd-2 (gd of herpes simplex virus type 2). although some differences were noted, particularly in the methionine and alanine profiles for gd-1 and gd-2, the amino a ... | 1984 | 6317892 |
| "recurrent herpes zoster": an unproved entity? | physicians sometimes misdiagnose recurrent zosteriform herpes simplex virus (hsv) skin infections as "zoster" or "recurrent zoster." misdiagnosis can lead to inappropriate therapy with potentially harmful consequences, particularly in patients with ophthalmitis or immunosuppression, in which early institution of the correct antiviral therapy may be crucial. we report three patients who were originally misdiagnosed to have recurrent herpes zoster skin infections before we cultured hsv from their ... | 1984 | 6327783 |
| secretory iga antibody in cervicovaginal secretions from women with genital infection due to herpes simplex virus. | sequential samples of cervicovaginal secretions from women with untreated first and recurrent episodes of genital infection due to herpes simplex virus type 2 (hsv-2) were assayed for iga antibodies to hsv-2 by using fluorescent antibodies to human secretory piece (siga) and human iga. among women with first-episode genital herpes, siga antibody to hsv-2 was detected in 20 of 31 women from whom hsv was isolated from the cervix, compared with four of 13 women from whom it was not (p less than .05 ... | 1984 | 6327834 |
| detection of herpes simplex virus intertypic recombinant genomes in infected cell dna. | intertypic recombination between herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) was detected using dna from mixedly infected cells. because hsv-1 and hsv-2 share a 50% base sequence homology along the genome but have markedly different dna restriction enzyme cleavage patterns, recombination events can be detected and quantified by analysis of restriction endonuclease digests for the presence of novel dna fragments. we have used this technique to quantify the degree of interference by hsv-2 ... | 1984 | 6323508 |
| mapping of the structural gene for the herpes simplex virus type 2 counterpart of herpes simplex virus type 1 glycoprotein c and identification of a type 2 mutant which does not express this glycoprotein. | the gene encoding glycoprotein f (gf) of herpes simplex virus type 2 (hsv-2) was mapped to the region of the viral genome from 0.62 to 0.64 map units. this region is colinear with, and partially homologous to, the region of the hsv-1 genome previously shown to encode gc. mapping of the gf gene was done by insertion of hsv-2 dna fragments into the thymidine kinase gene of an hsv-1 virus and screening of the resultant recombinant viruses for the expression of gf. in this way, dna sequences necessa ... | 1984 | 6321760 |
| mapping of a herpes simplex virus type 2-encoded function that affects the susceptibility of herpes simplex virus-infected target cells to lysis by herpes simplex virus-specific cytotoxic t lymphocytes. | a function(s) involved in the altered susceptibility of herpes simplex virus type 2 (hsv-2)-infected cells to specific lysis by cytotoxic t lymphocytes was mapped in the s component of hsv-2 dna by using hsv-1 x hsv-2 intertypic recombinants (rh1g44, rs1g25, r50bg10, a7d, and c4d) and hsv-1 mp. target cells infected with r50bg10, a7d, and c4d exhibited reduced levels of cytolysis, as did hsv-2-infected cells, whereas rh1g44 and rs1g25 recombinant-infected and hsv-1 mp-infected cells showed level ... | 1984 | 6321762 |
| heterogeneity in subregions of the terminal repeats of herpes simplex virus type 2 dna. | we constructed recombinant plasmids from the 0.5 molar terminal fragments of herpes simplex virus type 2 (hsv2) dna. physical mapping of three different l-terminal clones demonstrated one, two or three copies of the terminally redundant a repeat. the nucleotide sequence at the end of the terminal a repeats was shown to be identical in these three cloned hsv2 genomic l-termini and closely resembled the internal a' repeat sequence reported previously by davison and wilkie. virtually all clones tha ... | 1984 | 6321396 |
| herpes simplex virus type-selective enzyme-linked immunosorbent assay with helix pomatia lectin-purified antigens. | helix pomatia lectin-purified antigens with specific reactivity to herpes simplex virus type 1 (hsv-1) and hsv-2 antibodies in human sera were used in an enzyme-linked immunosorbent assay. the type specificity of the antigens was assessed by double immunodiffusion precipitation in gel against rabbit hsv-1 and hsv-2 hyperimmune sera, and by enzyme-linked immunosorbent assay with human reference sera containing antibodies to either type of hsv. fifty-two sera from patients with documented infectio ... | 1984 | 6321548 |
| early interactions between human cytomegalovirus and cells. | adsorption of cytomegalovirus (cmv) to human fibroblasts was not inhibited by preincubation with other herpes viruses (hsv-1, hsv-2, vzv). transport of virus to the nucleus was studied using virus labelled with 3h-thymidine. radioactivity was found in the nucleus 20 minutes after virus had been added to the cells. in order to assess the expression of the virus genome, synthesis of early (ea) and late (la) antigens was studied. assayed on permissive human lung fibroblasts, a plaque purified virus ... | 1984 | 6320772 |
| recovery of herpes simplex virus genetic information from human trigeminal ganglion cells following superinfection with herpes simplex virus type 2 temperature-sensitive mutants. | explant cultures of human trigeminal ganglia were derived from 36 individuals. those cultures which failed to release herpes simplex virus (hsv) spontaneously were superinfected at various times after establishment in vitro with a range of hsv-2 temperature-sensitive (ts) mutants. eight cultures from six individuals contained hsv-specific genetic information which could be detected or rescued following superinfection. restriction enzyme analysis of ts+ virus recovered from the ganglia of two ind ... | 1984 | 6319568 |
| class-specific antibody responses to early and late antigens of varicella and herpes simplex viruses. | antibody responses to early antigens of varicella-zoster virus (vzv), simian varicella virus, and herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were studied in primary, secondary, and latent infections. igg antibody responses to the early antigens occurred in primary and secondary vzv and hsv infections, and antibodies to early antigens were also demonstrable in healthy individuals with latent vzv and hsv infections, indicating that the presence of antibodies to early antigens cannot be t ... | 1984 | 6319583 |
| incidence of herpes simplex virus types 1 and 2 in penile lesions of college men. | herpes simplex virus (hsv) was isolated from penile lesions of 15 college men. using (e)-5-(2-bromovinyl)-2'-deoxyuridine sensitivity, monoclonal antibody immunofluorescence, and restriction endonuclease ecori digestion of viral dna, 4 of 15 (26%) isolates were found to be hsv-1, and 11 of 15 (74%) isolates were found to be hsv-2. it is likely that some of the genital hsv-1 infections are related to oral genital contact, but this fact could not be established for all cases, since the females in ... | 1984 | 6319589 |
| ultrastructural localization of gold in macrophages and mast cells exposed to aurothioglucose. | the ultrastructural localization of gold in resting, stimulated, and activated mouse peritoneal cells exposed in vivo and in vitro to aurothioglucose was examined by a photochemical technique. activated macrophages from herpes simplex virus type 2 infected mice showed the heaviest accumulation of gold, located in phagolysosomes. gold was also visualized in granules of mast cells and in nuclei of disintegrated cells. no gold was found in polymorphonuclear leucocytes and in lymphocytes. the mechan ... | 1984 | 6420183 |
| topical treatment of cutaneous herpes simplex virus infection in hairless mice with (e)-5-(2-bromovinyl)-2'-deoxyuridine and related compounds. | (e)-5-(2-bromovinyl)-2'-deoxyuridine (bromovinyldeoxyuridine) was found to suppress the development of herpetic skin lesions and the paralysis and mortality associated therewith in hairless mice inoculated intracutaneously with herpes simplex virus type 1. this protective effect was achieved with bromovinyldeoxyuridine applied topically at 1, 3, or 10% in either dimethylsulfoxide (dmso), beeler base, tween-glycerol-water, 5% azone (1-dodecylazacycloheptan-2-one) in water, or 5% azone in dmso. th ... | 1984 | 6486759 |
| broad-spectrum synergistic antiviral activity of selenazofurin and ribavirin. | the antiviral effects of selenazofurin (2-beta-d-ribofuranosylselenazole-4-carboxamide, selenazole), ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), and 3-deazaguanosine (6-amino-1-beta-d-ribofuranosylimidazo-[4.5-c]pyridin-4(5h)-one) were investigated separately and in various combinations in an in vitro study. the combination interactions were evaluated at seven drug concentrations, graphically (isobolograms) or by using fractional inhibitory concentration indices against mump ... | 1984 | 6517540 |
| recurrences after first episodes of genital herpes in patients treated with topical acyclovir cream. | the effect of topical acyclovir treatment of first episode genital herpes on the time to first recurrence in a group of 42 patients receiving either acyclovir or placebo was investigated. topical acyclovir treatment had no effect on time to first recurrence in patients with either first episode hsv-1 or hsv-2 infections. there was no significant difference in the time to first recurrence in patients with either true primary or initial genital infections. however, the time to first recurrence in ... | 1984 | 6517563 |
| epidemiological aspects of cervical cancer in tropical africa. | cervical cancer is one of the most common malignant tumours in tropical africa accounting, for example, for nearly one-quarter of female cancer cases overall in uganda. the disease is likely to be under-reported and available incidence rates are probably gross underestimates. spread is through sexual contacts. both female and male promiscuity and a low standard of sexual hygiene lead to a high incidence within a given community. a number of investigations show that the risk of cervical cancer va ... | 1984 | 6399280 |
| carcinoma of the cervix: can a viral etiology be confirmed? | the etiological role of viruses in various animal cancers is no longer in doubt; a similar role in human cancers is, however, yet to be confirmed. indeed, until recently, virus infections were considered to be responsible for only a small proportion of the clinical states encountered in humans and the view that viruses may be etiological agents for human malignant tumours was not seriously entertained. with the introduction of more advanced techniques in immunovirology, the role of viruses in cl ... | 1984 | 6399281 |
| enhanced efficacy of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine in combination with beta-interferon against herpes simplex virus type 2 in mice. | the acyclic nucleoside 9-(1,3-dihydroxy-2- propoxymethyl )guanine (dhpg) and natural mouse interferon beta ( muifn -beta) were evaluated for their efficacy alone and in combination against herpes simplex virus type 2 systemic infections in mice. intraperitoneally infected animals were treated once a day with the drugs at various concentrations for 5 days starting 24 h after inoculation. dhpg was injected subcutaneously at doses of 0.7 to 6 mg/kg. muifn -beta was given intraperitoneally at doses ... | 1984 | 6610387 |
| synthesis of some phosphonates with antiherpetic activity. | several keto phosphonates, phosphonoacetates , and dialkyl phosphonates containing (aryloxy)aryl groups were synthesized and evaluated for antiherpteic activity. two of the most active compounds, 12 and 16, were evaluated topically in the mouse vaginal model against herpes simplex virus (hsv) type 2. compound 16 exhibited an increased survival rate, as well as increased survival time. evaluation of 16 in the guinea pig skin test against hsv-2 produced a reduction in virus titer, as well as in me ... | 1984 | 6325695 |
| immunogenicity of herpes simplex virus glycoproteins gc and gb and their role in protective immunity. | the relative antigenicity of the individual herpes simplex virus type 1 (kos) glycoproteins gc and gb was analyzed in balb/c mice by using kos mutants altered in their ability to present these antigens on cell surface membranes during infection. the mutants employed were as follows: syn ld70 , a non-temperature-sensitive mutant defective in the synthesis of cell surface membrane gc; tsf13 , a temperature-sensitive mutant defective in the processing of the precursor form of gb to the mature cell ... | 1984 | 6328010 |
| physical mapping of the herpes simplex virus type 2 nuc- lesion affecting alkaline exonuclease activity by using herpes simplex virus type 1 deletion clones. | the nuc- lesion affecting alkaline exonuclease activity in the herpes simplex virus type 2 (hsv-2) mutant ts1348 had previously been mapped to the ecori-d restriction enzyme fragment of hsv-1. eight clones with deletions representing most of hsv-1 ecori fragment d were selected with lambda gtwes hybrids. these clones were tested for their ability to rescue the alkaline exonuclease activity of hsv-2 nuc- ts1348 virus. the sequences colinear with the hsv-2 nuc- lesion were found to map between 0.1 ... | 1984 | 6328043 |
| characterization of an aphidicolin-resistant mutant of herpes simplex virus type 2 which induces an altered viral dna polymerase. | the replication of wild-type herpes simplex virus type 2 (hsv-2) was very sensitive to aphidicolin, a specific inhibitor of eukaryotic alpha-type dna polymerases; viral dna synthesis was strongly inhibited by 1 microgram/ml of aphidicolin, but the synthesis of early viral polypeptides was not affected. using aphidicolin as the selective agent, aphidicolin-resistant ( aphr ) viruses were isolated from hsv-2 strain 186. all of these plaque isolates induced altered viral dna polymerases which were ... | 1984 | 6328755 |