Publications

reversibility of the stabilization effect of sodium molybdate on uterine estrogen and progesterone receptors of the vervet monkey.	sodium molybdate affected the stability of vervet monkey (cercopithecus aethiops pygerythrus) uterine estrogen (er) and progesterone (pr) receptors. yields of receptors were invariably higher (20-40%) when cytosols were prepared in the presence of 10mm sodium molybdate. no changes were observed in the binding affinities for the natural ligands as reflected in dissociation constants. receptor-ligand association at 0 degrees c and 20 degrees c was not affected in the presence or absence of molybda ...	1985	4057178
activation of metastatic potential in african green monkey kidney cell lines by prolonged in vitro culture.	studies on the tumorigenicity of vero kidney cells of cercopithecus aethiops monkey origin were extended to various passage levels of bsc-1 aneuploid cells and to low passage cv-1 diploid cells (derived also from c. aethiops monkey kidney). it was found that bsc-1 cells -- like vero cells -- showed increased tumorigenicity with increasing passage level in antithymocyte globulin (atg) treated newborn rats and in nude mice. cells passaged over 250 times in cultures formed invasive adenocarcinomas ...	1985	4066602
[activity of the nucleolus-organizing regions of the chromosomes of normal and transformed spleen cells in the african green monkey].	the activity of nucleolus organizer regions (nor's) of chromosomes in the african green monkey cells has been studied before and after the transformation. in cells before their transformation, one or two chromosomes entering into association and possessing nors are involved in the formation of one nucleolus. in transformed cells no correlation between the numbers of nucleoli in interphase and those of chromosomes possessing nors was revealed in metaphase plates. it is supposed that in chromosome ...	1985	4071663
behavioral aspects of serotonin-dopamine interaction in the monkey.	the effects of serotonin (5-hydroxytryptamine; 5-ht) antagonists and 5-ht uptake inhibitors on the behavioral response to amphetamine and haloperidol in monkeys (cercopithecus aethiops) were investigated. amphetamine increased locomotor activity and reactivity and induced repetitive movements of head, limbs and trunk, but no oral hyperkinesia. haloperidol induced dystonia and parkinsonism. pretreatment with the 5-ht antagonists cyproheptadine and mianserin increased amphetamine-induced locomotor ...	1985	4085556
genital signalling and the coexistence of male vervet monkeys (cercopithecus aethiops pygerythrus).	to ascertain the social significance of male genital colouration two troops of vervets, one free-ranging and one caged, were observed. it was found that genital signals were associated with intermale agonism. it is suggested that this communicatory system regulates the behaviour of competitors and may thereby have facilitated the evolution of a multimale social system.	1985	3833622
morphological changes in cho and vero cells treated with t-2 mycotoxin. correlation with inhibition of protein synthesis.	exposure of chinese hamster ovary and african green monkey kidney cells to t-2 mycotoxin resulted in several morphological changes which were related to inhibition of protein synthesis, the basic in vitro mechanism of action of the toxin. these changes, which occurred in both cell types, included disassociation of polysomes and mitochondrial cristae alterations. in addition, cho cells displayed membrane bleb formations similar to those found in cho cells after exposure to established inhibitors ...	1985	3836022
nucleotide sequence analysis and enhancer function of long terminal repeats associated with an endogenous african green monkey retroviral dna.	the nucleotide sequence and enhancer activity of the long terminal repeats (ltrs) associated with a cloned endogenous african green monkey (agm) retroviral dna designated as lambda-agm-1 was studied. a unique feature of the endogenous agm proviral ltrs was the presence of multiple copies of two types of directly repeating units in the u3 region: 16 8-base-pair (bp) repeats were present in the 5' ltr and 12 were present in the 3' ltr which were bound by a 6-bp perfect direct repeat; tandem duplic ...	1985	3861934
protein synthesis by mammalian cells treated with c-3-modified analogs of the 12,13-epoxytrichothecenes t-2 and t-2 tetraol.	modification at the c-3 position of the trichothecenes t-2 and t-2 tetraol affected their ability to inhibit protein synthesis in african green monkey kidney (vero) and mouse erythroleukemia cells. replacement of the 3-hydroxyl of t-2 with hydrogen caused a 24-fold decrease in activity, whereas acetylation resulted in a 500-to 1,000-fold decrease. protection of the 3-hydroxyl with a tetrahydropyranyl moiety gave an analog that was 37-fold more inhibitory to vero than to mouse erythroleukemia cel ...	1985	3866515
recombination and deletion of sequences in shuttle vector plasmids in mammalian cells.	shuttle vector plasmids were constructed with directly repeated sequences flanking a marker gene. african green monkey kidney (agmk) cells were infected with the constructions, and after a period of replication, the progeny plasmids were recovered and introduced into bacteria. those colonies with plasmids that had lost the marker gene were identified, and the individual plasmids were purified and characterized by restriction enzyme digestion. recombination between the repeated elements generated ...	1985	3869955
a growth regulatory factor that can both inhibit and stimulate growth.	a growth inhibitor that is produced by bsc-1 cells (african green monkey kidney epithelial cells) has been isolated from conditioned medium. it has been purified by gel chromatography and high performance liquid chromatography. it appears to be a protein with a relative molecular mass (mr) of 24 000. it is extremely active as a growth inhibitor with some cells, but not with others. approximately 50% inhibition of thymidine incorporation is observed with ccl64 cells at 0.05 ng/ml and with bsc-1 c ...	1985	3878272
circadian rhythm of hmg-coa reductase and insulin in african green monkeys.	hmg-coa reductase in hepatic microsomes and serum insulin display circadian rhythm in two strains of cercopethicus aethiops. grivets develop higher levels of serum cholesterol than vervets fed cholesterol. males (n = 20/gp) were adapted to a light cycle (7:30 a.m.-8:30 p.m.) for 60 days and fed a non-cholesterol diet at 8:30 a.m. and 2:00 p.m. vervet acrophase, reductase activity was synchronized to serum insulin units with a specific activity of 1480 pmol/min/mg protein changing by 7.5-fold fro ...	1985	3888223
the effects of ketamine anesthesia on glucose clearance in african green monkeys.	ketamine hydrochloride's effect on glucose clearance-insulin secretion during intravenous glucose tolerance testing was studied in five african green monkeys. animals were tested with ketamine anesthesia and then had indwelling cannulas implanted and were retested both in the presence and absence of ketamine anesthesia. serum glucose and insulin concentrations were determined. there were no significant differences in the glucose clearance rate (k value), basal glucose and insulin concentrations, ...	1985	3889340
the pulpal response to dilute citric acid smear removers.	the aim of this study was to determine the pulpal response to three dilute citric acid smear removers. forty-eight vervet monkeys (cercopithecus aethiops pygerythrus), in three groups of sixteen animals provided 384 tooth specimens for the histological evaluation of the pulpal response at 3, 31, and 59 days post-operatively. labial class v cavities were prepared in the maxillary and mandibular incisor teeth. the pulpal responses elicited by 1% aqueous citric acid, 1% citric acid in 30% ethanol a ...	1985	3891932
cytotoxicity testing of a dentin bonding system.	the cytotoxicity of a newly developed dentin bonding system was examined in african green monkey kidney cells and human embryonic lung cells. a tissue culture agar overlay procedure, a longitudinal study, and a cell replication assay were employed to evaluate cytotoxicity. several of the components were discovered to be cytotoxic when tested individually in vitro. however, when used in the prescribed manner, little or no cytotoxic effect was elicited. the results of this study support further te ...	1985	3892412
hormonal responses to gonadotrophin releasing hormone during testicular development in male african green monkeys.	reproductive development in male african green monkeys was characterized by evaluating both luteinizing hormone (lh) and testosterone (t) before and after gonadotrophin releasing hormone (gnrh) stimulation in relation to the physical maturation of the testis. there were lh responses to gnrh at all ages studied, but the failure of some animals to respond at earlier ages suggested developmental changes in the responsiveness of the pituitary. the t secretion developed progressively but did not reac ...	1985	3908686
[gallbladder measurements in the green monkey (cercopithecus aethiops) and their dependence on liver and body weight].		1985	3917106
experimental leprosy in three species of monkeys.	eleven mangabey monkeys inoculated with mycobacterium leprae developed lepromatous-type leprosy. nine of the mangabeys were inoculated with m. leprae isolated from a mangabey with naturally acquired lepromatous leprosy. immune function was depressed in some of these animals after dissemination of the disease. two mangabeys developed lepromatous leprosy after inoculation with human m. leprae passaged in an armadillo. three rhesus and three african green monkeys inoculated with mangabey-derived m. ...	1985	3917577
the effect of apicoectomy before replantation on periodontal and pulpal healing in teeth in monkeys.	the effect of resection of the apical part of the root before replantation upon periodontal and pulpal healing was studied in 7 green vervet monkeys (cercopithecus aethiops) using teeth with incomplete and complete root formation. maxillary central incisors, mandibular lateral incisors and first and second mandibular molars were used in this experiment. on one side, the tooth was extracted and replanted with an intact root while the contralateral tooth had 2 mm of the apex resected for the purpo ...	1985	3920162
the heat shock consensus sequence is not sufficient for hsp70 gene expression in drosophila melanogaster.	a hybrid gene in which the expression of an escherichia coli beta-galactosidase gene was placed under the control of a drosophila melanogaster 70,000-dalton heat shock protein (hsp70) gene promoter was constructed. mutant derivatives of this hybrid gene which contained promoter sequences of different lengths were prepared, and their heat-induced expression was examined in d. melanogaster and cos-1 (african green monkey kidney) cells. mutants with 5' nontranscribed sequences of at least 90 and up ...	1985	3920509
recombinant huifn-gamma prevents herpes simplex keratitis in african green monkeys: demonstration of synergism with recombinant huifn-alpha 2.	human interferon gamma (huifn-gamma) was found to prevent herpes-simplex virus (hsv 1) induced keratitis in monkey eyes, when administered topically at concentrations of greater than or equal to 3 x 10(5) reference units/ml. protective efficacy demonstrated with lower concentrations of huifn-gamma in combination with low titers of huifn-alpha provided evidence of synergistic interferon activity in vivo. tolerance problems observed in eyes affected by virus inoculation seem to be attributable to ...	1985	3929044
wild african green monkeys of barbados are htlv negative.		1985	2857892
antibodies to simian t-lymphotropic retrovirus type iii in african green monkeys and recognition of stlv-iii viral proteins by aids and related sera.		1985	2860515
production and characterization of monoclonal antibodies against clostridium perfringens type a enterotoxin.	hybridomas secreting monoclonal antibodies (mabs) specific for clostridium perfringens type a enterotoxin were produced by fusion of p3x63ag8.653 myeloma cells with spleen cells from balb/c mice immunized with purified enterotoxin. wells containing hybridomas secreting immunoglobulin g (igg) antibodies against enterotoxin were specifically identified by an indirect enzyme-linked immunosorbent assay (elisa), and 10 elisa-positive hybridomas were selected and cloned twice by limiting dilution. all ...	1985	2865210
isolation of simian retroviruses closely related to human t-cell leukemia virus by establishment of lymphoid cell lines from various non-human primates.	simian retroviruses closely related to human t-cell leukemia virus (htlv) were isolated by establishing virus-producing lymphoid cell lines from 7 species of non-human primates. by co-cultivation with human umbilical cord-blood cells and/or in the presence of interleukin-2, lymphoid cell lines were successfully established from the chimpanzee. african green monkey, pig-tailed macaque, red-faced macaque, formosan monkey, japanese monkey and bonnet monkey that had antibodies against htlv antigens. ...	1985	2579038
characterization of angiotensin ii binding sites in african green monkey uterus.	the observation that there are significant differences in the concentration, affinity, and specificity of both central nervous system (cns) and peripheral angiotensin receptors among several different mammalian species, including the african green monkey, led to the detailed analysis of 125i-angiotensin ii binding in the uterus of the african green monkey. the bmax for angiotensin receptors in uterine tissue from this species is 56.6 +/- 8.7 fmole per mg protein. the kd for angiotensin ii is .60 ...	1985	2981383
expression of an ouabain resistance gene in transfected cells. ouabain treatment induces a k+-transport system.	we have investigated the expression of a cloned mouse gene which confers ouabain resistance to african green monkey kidney (cv1) cells. cv1 cells carrying the transfected ouabain resistance (ouar) gene express an ouabain-inducible k+-transport system. this k+-transport system is not a normal (na,k)-atpase since plasma membranes prepared from the transfected cells have significantly reduced na+-stimulated atpase activity. rna sequences homologous to the transfected gene are observed in abundance ...	1985	2981832
two separable functional domains of simian virus 40 large t antigen: carboxyl-terminal region of simian virus 40 large t antigen is required for efficient capsid protein synthesis.	the carboxyl-terminal portion of simian virus 40 large t antigen is essential for productive infection of cv-1 and cv-1p green monkey kidney cells. mutant dla2459, lacking 14 base pairs at 0.193 map units, was positive for viral dna replication, but unable to form plaques in cv-1p cells (j. tornow and c.n. cole, j. virol. 47:487-494, 1983). in this report, the defect of dla2459 is further defined. simian virus 40 late mrnas were transcribed, polyadenylated, spliced, and transported in dla2459-in ...	1985	2982029
selectivity of interferon action in simian virus 40-transformed cells superinfected with simian virus 40.	treatment of african green monkey kidney cv-1 cells with human alpha interferons before infection with simian virus 40 (sv40) inhibited the accumulation of sv40 mrnas and sv40 t-antigen (tag). this inhibition persisted as long as the interferons were present in the medium. sv40-transformed human sv80 cells and mouse sv3t3-38 cells express tag, and interferon treatment of these cells did not affect this expression. sv80 and sv3t3-38 cells which had been exposed to interferons were infected with a ...	1985	2983099
serotypic characterization of rotaviruses derived from asymptomatic human neonatal infections.	nineteen rotavirus strains derived from asymptomatic neonates (seven from england, five from australia, two from venezuela, and five from sweden) were successfully cultivated in primary african green monkey kidney cell cultures, serotyped by plaque reduction neutralization tests, subgrouped by indirect enzyme-linked immunosorbent assay, and electropherotyped by polyacrylamide gel electrophoresis. all 19 strains were shown to fall into one of the four known human serotypes; serotype 1 (all venezu ...	1985	2984247
the kpni family of long interspersed nucleotide sequences is present on discrete sizes of circular dna in monkey (bsc-1) cells.	discretely sized molecules of small circular dnas in african green monkey kidney (bsc-1) cells contain nucleotide sequences homologous to the kpni family of long interspersed repetitive nucleotide sequences. the size distribution of these kpni family-containing circular dnas differs markedly from those of bsc-1 cell circular dnas containing either the alu family of short interspersed nucleotide sequences or the alpha-satellite family of tandemly repeated sequences. the structures of several clon ...	1985	2984431
simian agent 12 is a bk virus-like papovavirus which replicates in monkey cells.	we have begun to characterize the genomic structure and replication of the baboon papovavirus simian agent 12 (sa12). we have defined a wild-type clone of sa12 (sa12 wt100) by plaque purification from a heterogeneous stock. the functional map of sa12 wt100 can be aligned with those of the other primate papovaviruses by assigning one of the two ecori sites as 0/1.0 map units. the origin of bidirectional viral dna replication maps near 0.67 map units, consistent with the limits of sequences homolo ...	1985	2985810
mutations near the carboxyl terminus of the simian virus 40 large tumor antigen alter viral host range.	we report the characterization of three mutants of simian virus 40 with mutations that delete sequences near the 3' end of the gene encoding large tumor antigen (t antigen). two of these mutants, dl1066 and dl1140, exhibit an altered viral host range. wild-type simian virus 40 is capable of undergoing a complete productive infection on several types of established african green monkey kidney lines, including bsc40 and cv1p. dl1066 and dl1140 grow on bsc40 cells at 37 degrees c. however, both mut ...	1985	2985819
persistently infected cultures as a source of hepatitis a virus.	primary african green monkey kidney, continuous african green monkey kidney cell line bs-c-1, and buffalo green monkey kidney cultures were infected with a uniform inoculum of hepatitis a virus (hav). although both the cell line bs-c-1 and primary african green monkey kidney cultures produced useful amounts of virus, hav was detected earlier and in greater quantities in primary african green monkey kidney cultures. a persistently infected primary african green monkey kidney culture was developed ...	1985	2988436
retroviruses associated with leukemia and ablative syndromes in animals and in human beings.	t-lymphotropic retroviruses of cats cause lymphopenia and immunosuppression and represent the major cause of death in that species. similarly htlv-i which is t4 tropic is associated with an increased risk for development of infectious disease in regions where the virus is endemic. since htlv-i is also believed to be transmitted by blood and by sexual intercourse we considered the possibility that a variant form of htlv might cause aids. the identification of cross-reactive antibodies to htlv-i-m ...	1985	2990682
the association of the interspersed repetitive kpni sequences with the nuclear matrix.	the kpni sequences constitute the dominant, long, interspersed repetitive dna families in primate genomes. these families contain related, but nonidentical sequence subsets, some of which border functional gene domains and are transcribed into rna. to test whether these sequences perform an organizational function in the nucleus, their association with the nuclear matrix has been examined in african green monkey cells. dnase i treatment depleted the residual matrix of most of the kpni 1.2- and 1 ...	1985	2991242
transcription factor sp1 recognizes promoter sequences from the monkey genome that are simian virus 40 promoter.	a 440-base-pair fragment of african green monkey genomic dna shares homology with the transcriptional regulatory region of simian virus 40 (sv40) and has been reported to direct transcription in vivo. we find that two regions within this fragment bind the promoter-specific cellular transcription factor sp1 and are protected in dnase protection ("footprinting") experiments. as in sv40, binding occurs in regions containing multiple copies of the sequence gggcgg. these regions, when fused to the pr ...	1985	2991898
covalent cross-linking of vasoactive intestinal peptide (vip) to its receptor in intact colonic adenocarcinoma cells in culture (ht 29).	[125i]monoiodinated vasoactive intestinal peptide (125i-vip) was cross-linked with human colonic adenocarcinoma cells (ht29 cells) grown as a monolayer using dithiobis(succinimidylpropionate) as cross-linking reagent. the cross-linked polypeptides were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. a major polypeptide of mr = 67 000 was characterized and it behaved like a high-affinity binding site for vip according to the following data. the concentra ...	1985	2992966
association of phosphorylated simian virus 40 t-antigen with subnuclear fractions of infected and transformed cells.	to define the roles of subnuclear structure in sv40 infection, the relative distribution of t-antigen (t-ag) in various subnuclear fractions obtained from both lytically infected and transformed african green monkey kidney cells was determined. depending on the differential sensitivity of nuclear t-ag to extraction by salt and detergent, nuclear t-ag could be separated into nucleoplasmic t-ag, salt-sensitive t-ag and matrix-bound t-ag subclasses. at least fivefold less matrix-bound t-ag was foun ...	1985	2995093
antigens of human t-lymphotropic virus type iii/lymphadenopathy-associated virus.	antigens encoded by the gag and env genes of the human t-lymphotropic virus type iii/lymphadenopathy associated virus (htlv-iii/lav) include a p55 gag polyprotein that yields p24 as the major virus core protein, and an env gene polyprotein, gp 160, that produces gp 120, the most immunogenic protein in humans, at the amino terminus. although its use is limited to research laboratories due to the cost and specialized procedures involved, the analysis of sera by radioimmunoprecipitation and sodium ...	1985	2996402
isolation and adaptation characteristics of hepatitis a virus in primary african green monkey kidney cells: production of antigen useful for elisa serology.	four strains of hepatitis a virus (hav) were isolated from four fecal samples of patients with type a hepatitis by using primary african green monkey kidney (pagmk) cells or frhk-4 cells. in all four samples viral antigen became detectable in pagmk cells at the 3rd passage level after 9 weeks of incubation; detectable levels of antigen were reached earlier in frhk-4 cells. an enzyme-linked immunosorbent assay (elisa) was used to detect hav antigen (hav-ag). blocking experiments with negative and ...	1985	2997078
one role for dna methylation in vertebrate cells is strand discrimination in mismatch repair.	although the occurrence of 5-methylcytosine (m5c) in dna is widespread, the function of this modified base remains unclear. at some specific sites it apparently has an effect in controlling gene expression, but many sites do not appear to be involved in this regulation. balanced against its regulatory usefulness at some sites is the mutational risk it imposes upon the cell. deamination of m5c can lead to its replacement by thymine (t). one possible role for excess methylation is strand discrimin ...	1985	2997788
isolation of t-lymphotropic retrovirus related to htlv-iii/lav from wild-caught african green monkeys.	present evidence suggests that the acquired immune deficiency syndrome (aids) emerged in central africa as a new disease in recent decades. this disease has recently approached epidemic proportions in many parts of the world. the etiologic agent of aids is believed to be the virus htlv-iii/lav, which has been proposed as having originated from a recent simian-human transmission in africa. this report describes the isolation of a designated stlv-iiiagm retrovirus closely related to htlv-iii/lav f ...	1985	2997923
recombinants between attenuated and virulent strains of poliovirus type 1: derivation and characterization of recombinants with centrally located crossover points.	recombinants with a centrally located crossover point were selected from crosses between poliovirus type 1 strains and intertypic (type 3/type 1) recombinants. two such recombinants were characterized in some detail. in one of them (v1/a1-6), the 5' half of the genome was derived from a virulent type 1 strain, while the 3' half came from an attenuated type 1 strain. the genome of the other recombinant (a1/v1-7) had the reverse organization, with the 5' and 3' halves being derived from the type 1 ...	1985	2998022
significance of glycosidases and phosphatases in cercopithecus and macaca cells.	when selected ratios of different glycosidases and phosphatases from primary monkey kidney cells or from monkey kidney cell lines are presented graphically, characteristic patterns do evolve. three different subtypes of vero cells show similar glycosidase patterns. the vero subtypes tested show glycosidase patterns that are closely similar to those of primary cells of cercopithecus aethiops. glycosidase patterns of bs-c-1 and cv-1 cells are less similar to those of primary cercopithecus cells th ...	1985	2998900
a shuttle vector plasmid for studying carcinogen-induced point mutations in mammalian cells.	we have constructed a shuttle vector plasmid for studying mutagenesis in mammalian cells. the plasmid replicates in cell lines permissive for sv40 virus as well as in the bacterium escherichia coli and carries a bacterial suppressor trna gene (supf) that can serve as a mutagenesis marker. the plasmid replicates as efficiently as sv40 virus in african green monkey kidney cv1 cells, indicating that all traces of the inhibitory sequences normally found in pbr322 and its derivatives have been remove ...	1985	2998945
adult t-cell leukemia-like disease in monkey naturally infected with simian retrovirus related to human t-cell leukemia virus type i.	spontaneous t-cell leukemia similar to human adult t-cell leukemia (atl) was found in an african green monkey naturally infected with simian retrovirus closely related to human t-cell leukemia virus type i (htlv-i). monoclonal integration of the simian retrovirus was detected in the primary leukemic cells, suggesting an association of the retrovirus with atl-like leukemia in the monkey.	1985	3001009
effect of chlordane on influenza type a virus and herpes simplex type 1 virus replication in vitro.	the effect of chlordane on the susceptibility of madin-darby canine kidney cells and african green monkey kidney cells to infection with influenza type a/pr/8/34 (hon1) virus and herpes simplex type 1 virus was determined. exposure of both cell lines to various concentrations of chlordane for 24 h at 37 degrees c (acute exposure) effected a marked reduction in the efficiency of influenza type a virus infection, except at a dose of 0.025 ppm. acute exposure of the monkey cells did not alter their ...	1985	3001327
control of ribonucleic acid function by oligonucleoside methylphosphonates.	oligodeoxyribonucleoside methylphosphonates contain nonionic 3'-5' linked methylphosphonate internucleotide bonds in place of the normal charged phosphodiester linkage of natural nucleic acids. these oligomers are resistant to nuclease hydrolysis, can pass through the membranes of mammalian cells in culture and can form stable hydrogen-bonded complexes with complementary nucleotide sequences of cellular rnas such as mrna. the oligomers are readily synthesized on insoluble polymer supports. their ...	1985	3002493
surface expression of influenza virus neuraminidase, an amino-terminally anchored viral membrane glycoprotein, in polarized epithelial cells.	we have investigated the site of surface expression of the neuraminidase (na) glycoprotein of influenza a virus, which, in contrast to the hemagglutinin, is bound to membranes by hydrophobic residues near the nh2-terminus. madin-darby canine kidney or primary african green monkey kidney cells infected with influenza a/wsn/33 virus and subsequently labeled with monoclonal antibody to the na and then with a colloidal gold- or ferritin-conjugated second antibody exhibited specific labeling of apica ...	1985	3016520
nucleoside pools of acyclovir-treated herpes simplex type 1 infected cells.	nucleoside pools of herpes simplex type 1 (hsv-1)-infected and uninfected african green monkey kidney (gmk) cells and human fetal lung fibroblasts (hl) have been analysed with high-performance liquid chromatography (hplc). the only nucleosides found in measurable amounts were deoxythymidine (dthd) and adenosine (ado). the dthd pool seemed to be greater in gmk cells than in hl cells. dthd was also the only nucleoside excreted into the medium. hsv-1 infection reduced the dthd concentration of gmk ...	1985	3160302
parvovirus-like particles associated with diarrhea in unweaned piglets.	numerous parvovirus-like particles, 18 to 26 nm in diameter, were detected by electron microscopy in the intestinal contents of two to three week old piglets with mild to severe diarrhea, in six quebec pig herds. hemagglutination of guinea pig and african green monkey red blood cells was obtained with clarified intestinal contents. two isolates were found to be antigenically related to porcine and canine parvoviruses, while another differed from the porcine parvovirus using the hemagglutination- ...	1985	2412678
s-phase patterns of cyclin (pcna) antigen staining resemble topographical patterns of dna synthesis. a role for cyclin in dna replication?	the sequence of cyclin (proliferating cell nuclear antigen, pcna), antigen staining throughout the cell cycle of african green monkey kidney cells (bs-c-1) has been determined by indirect immunofluorescence using pcna autoantibodies specific for this protein. patterns of cyclin staining observed between the beginning of s-phase and maximum dna synthesis are similar to those reported in human ama cells [(1985) proc. natl. acad. sci. usa 82, 3262-3266], while those detected thereafter are signific ...	1985	2415394
improved in situ hybridization and g-banding by pretreatment with denhardt's solution and gelatin-chrome alum.	various pretreatments of metaphase spreads were examined to obtain optimal dna labelling patterns while maintaining chromosome integrity during in situ hybridization procedures. preparations of african green monkey (agm) chromosomes fixed in methanol-acetic acid (cv-1 cell line) were treated by coating with denhardt's solution, dilute gelatin-chrome alum, nonfat instant dry milk dissolved in saline-citrate solution (ssc) and/or acetylation prior to denaturation of chromosomal dna in 70% formamid ...	1985	2417994
adaptation and characteristics of replication of a strain of avian reovirus in vero cells.	one of six australian strains of avian reovirus was adapted to replicate in an african green monkey kidney (vero) cell line. the cytopathic effect of the adapted strain in vero cells was characterised by syncytium formation occurring 12 hours post-infection, coincident with the production of progeny virus. the virus in vero cells was very cell-associated and longer incubation times were required for titration than in primary cultures of chicken kidney cells. methods for determination of the 50% ...	1985	18766925
effects of chronic fenfluramine on blood serotonin, cerebrospinal fluid metabolites, and behavior in monkeys.	the effects of long term (70 days) fenfluramine treatment on selected physiological and behavioral measures were examined in four adult male vervet monkeys (cercopithecus aethiops sabaeus). relative to pretreatment baseline values, whole blood serotonin (wbs) and cerebrospinal fluid 5-hydroxyindole acetic acid (5-hiaa) were reduced, cerebrospinal fluid homovanillic acid (hva) was unaltered, and aggressive and locomotor behavior were increased. both physiological and behavioral effects were rever ...	1986	2433707
isolation of low-copy-number sequences that neighbor satellite dna in mammals.	to investigate the role of satellite dna in eukaryotic genomes, we isolated from an african green monkey (cercopithecus aethiops) genomic library cloned segments containing the previously described deca-satellite linked to low-copy-number genomic sequences. three such clones were obtained. the low-copy-number sequences in the three clones do not cross-hybridize suggesting that they derive from different genomic loci. the structure of one of the clones, lambda mka, is described in detail. subclon ...	1986	3034734
reorganization of alpha-actinin and vinculin induced by a phorbol ester in living cells.	we have used fluorescent analogue cytochemistry, image intensification, and digital image processing to examine the redistribution of alpha-actinin and vinculin in living cultured african green monkey kidney (bsc-1) cells treated with the phorbol ester 12-o-tetradecanoylphorbol-13-acetate (tpa). before treatment, microinjected alpha-actinin shows characteristic distribution along stress fibers and at adhesion plaques; vinculin is localized predominantly at adhesion plaques. soon after the additi ...	1986	3082892
cell-cycle-dependent repair of damage in alpha and bulk dna of monkey cells.	excision repair of bulky chemical adducts in alpha dna of confluent cultures of african green monkey cells has previously been shown to be deficient relative to that in the overall genome. we have compared the removal of adducts produced by treatment with aflatoxin b1 (afb1) and n-acetoxy-2-acetylamino-fluorene (na-aaf) from alpha dna sequences in synchronized and exponentially growing cultures of monkey cells. proficient removal of afb1 adducts in alpha dna was observed in exponentially growing ...	1986	3088442
sulphoxidation of s-carboxymethyl-l-cysteine in the rhesus monkey (macaca mulatta), cynomologus monkey (macaca fascicularis), african green monkey (cercopithecus aethiops) and the marmoset (callithrix jacchus).	the metabolic pathways giving rise to the urinary metabolites of s-carboxymethyl-l-cysteine have been identified for the rhesus, cynomologus, african green and marmoset species of monkey. the formation of a sulphoxide metabolite from the sulphide precursor is a reaction important in these species. the metabolic profile displayed by the marmoset was distinct from the three old world species, with the rhesus and cynomologus being similar to man.	1986	3089678
high density lipoprotein accumulation in perfusates of isolated livers of african green monkeys. effects of saturated versus polyunsaturated dietary fat.	to determine whether altered hepatic secretion of hdl is part of the mechanism by which polyunsaturated fat lowers plasma hdl concentration, we have studied hdl secretion in the isolated perfused livers of african green monkeys fed an atherogenic diet containing either safflower oil as the polyunsaturated fat or butter as the saturated fat. during recirculating perfusion with a lipoprotein-free medium, livers from safflower oil-fed animals produced 21% less hdl mass on the average than those fro ...	1986	3090181
benzo[a]pyrene diol epoxide induces viral reactivation at concentrations that block dna elongation in mammalian cells.	the survival of uv-irradiated simian virus 40 (sv40) in cv-1p african green monkey kidney cells treated with (+/-)7 beta, 8 alpha-dihydroxy-9 alpha, 10 alpha epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (bp-diol epoxide i) was studied. enhanced survival of uv damaged sv40 was detected when cv-1p cells were treated with dose levels of bp-diol epoxide i corresponding to the exponential portion (0.33-1.11 microm) of a cv-1p cell survival curve. dose levels of bp-diol epoxide i corresponding to the shoul ...	1986	3017584
sequence and evolution of rhesus monkey alphoid dna.	analysis of rhesus monkey alphoid dna suggests that it arose by tandem duplication of an ancestral monomer unit followed by independent variation within two adjacent monomers (one becoming more divergent than the other) before their amplification as a dimer unit to produce tandem arrays. the rhesus monkey alphoid dna is a tandemly repeated, 343-bp dimer; the consensus dimer is over 98% homologous to the alphoid dimers reported for baboon and bonnet monkey, 81% homologous to the african green mon ...	1986	3018269
dna helicase activity of sv40 large tumor antigen.	large tumor antigen (t antigen) was extracted from sv40-infected african green monkey cells and purified to homogeneity by immunoaffinity chromatography. the purified t antigen preparations unwind dna duplices of greater than 120 bp in a reaction which is dependent on magnesium ions and atp hydrolysis. based on these and other properties of the reaction we classify this newly discovered enzymatic activity as a eukaryotic dna helicase. the helicase and the known atpase function of t antigen cosed ...	1986	3019672
stlv-i antibodies in feral populations of east african vervet monkeys (cercopithecus aethiops).	serum samples from feral populations of african green monkeys (cercopithecus aethiops) were screened for antibodies to the simian t-lymphotropic virus, type i (stlv-i). blood samples had been collected from 336 monkeys in 4 regions of central and southern kenya in 1978 and 1979, from 114 monkeys in central ethiopia in 1973, and from 85 monkeys from the kampala region of uganda in 1966. a total of 178/535 monkeys (33%) were seropositive (stlv-i+). only 4/114 monkeys (4%) from ethiopia were seropo ...	1986	3019900
dna engineering shows that nucleosome phasing on the african green monkey alpha-satellite is the result of multiple additive histone-dna interactions.	the mechanism underlying sequence-specific positioning of nucleosomes on dna was investigated. african green monkey alpha-satellite dna was reconstituted in vitro with histones. histone octamers were found to adopt one major and several minor positions on the satellite repeat unit, very similar to those positions found previously in vitro, demonstrating that sequence-specific histone-dna interactions are responsible for nucleosome positioning on this dna. in order to understand the nature of the ...	1986	3023642
intramolecular recombination between transfected repeated sequences in mammalian cells is nonconservative.	when plasmids carrying a fragmented gene with segments present as direct repeats are introduced into mammalian cells, recombination or gene conversion between the repeated sequences can reconstruct the gene. intramolecular recombination leads to the deletion of the intervening sequences and the loss of one copy of the repeat. this process is known to be stimulated by double-strand breaks. two current models for recombination in eucaryotic cells propose that the reaction is initiated by double-st ...	1986	3023937
the causes of false-positives encountered during the screening of old-world primates for antibodies to human and simian retroviruses by elisa.	sera from 526 old-world primates representing 50 different species were screened by elisa for antibodies to human t-lymphotropic viruses i and iii, and simian retrovirus type 1 (srv-1). about one-fourth of the sera were positive by elisa. there was a tendency, however, for the same sera to be positive for all three human and simian retroviruses. only about one in five of the elisa antibody-positive sera were confirmed to be positive by western blotting. false-positive elisa antibody tests were p ...	1986	3025237
nucleotide sequence of the bamhi repetitive sequence, including the hindiii fundamental unit, as a possible mobile element from the japanese monkey macaca fuscata.	clustered repeat units produced by bamhi digestion of genomic dna from the japanese monkey macaca fuscata [jmr(bamhi)] were sequenced by dideoxy dna sequencing. the nucleotide sequences of several individual repeats showed that the bamhi repeat contains the 170-bp hindiii element as an integral part, and that it has more than 90% homology with the hindiii repeat element [agmr(hindiii)] found in the genomic dna of the african green monkey. in the jmr(bamhi) repeat unit, the 170-bp hindiii element ...	1986	3027353
alpha dna in african green monkey cells is organized into extremely long tandem arrays.	we have determined the size of arrays formed by tandemly repeated monomers of alpha dna in african green monkey cells. dna fragments containing intact alpha dna arrays were generated by digestion of genomic dna with restriction endonuclease that do not have sites in the alpha dna consensus sequence. their size was determined by southern analysis and by sedimentation through neutral sucrose gradients followed by probing of each fraction for alpha sequences. the restriction fragments varied in siz ...	1986	3003109
new human t-lymphotropic retrovirus related to simian t-lymphotropic virus type iii (stlv-iiiagm).	this report describes serologic evidence for a virus similar to that known as simian t-lymphotropic virus type iii of african green monkeys (stlv-iiiagm) infecting apparently healthy people in senegal, west africa, and the isolation of virus from these individuals. serum samples from selected healthy west african people showed unusual serologic profiles when tested with antigens of htlv-iii/lav, the etiologic agent of aids, and of stlv-iiiagm. the samples reacted strongly with all of the major v ...	1986	3006256
varicella-like herpesvirus infections of nonhuman primates.	at least three distinct herpesviruses cause varicella like exanthematous diseases among nonhuman primates. spontaneous epizootics have resulted in high morbidity and mortality rates among cercopithecus aethiops, erythrocebus patas and macaca species in research colonies. mild infections have been observed in infant chimpanzees and a gorilla. this group of diseases is of interest not only because they are a threat to primate colonies, but also because their pathologic similarity to progressive hu ...	1986	3007865
an infectious cdna clone of the poliovirus sabin strain could be used as a stable repository and inoculum for the oral polio live vaccine.	viruses were recovered from hela s3 cells and african green monkey kidney (agmk) cells transfected with an infectious cdna clone of poliovirus vaccine sabin 1 strain. the viruses recovered from the different dna-transfected cells were tested for the biological characteristics of temperature sensitivity (rct marker), plaque size, and bicarbonate concentration dependency (d marker). the results revealed that the above properties were similar to those obtained from tests on the sabin 1 vaccine refe ...	1986	3008430
quantitation and kinetics of induced hsv-1 ocular shedding.	iontophoresis of 6-hydroxydopamine (6-hd) to the rabbit eye, followed by topical instillation of 2% epinephrine, induces ocular shedding of herpes simplex virus type 1 (hsv-1) reliably and with a high frequency in latently infected rabbits. rabbit eyes inoculated with hsv-1 (mckrae strain) showed dendritic lesions indicative of acute hsv infection and subsequently shed virus spontaneously at least once during days 20 to 39 postinoculation (p.i.). two iontophoretic conditions were employed. group ...	1986	3009094
evaluation of cell line 293 for virus isolation in routine viral diagnosis.	cell line 293, a continuous line of transformed human embryonic kidney cells, has been recognized for its sensitivity in the isolation of adenoviruses, particularly the fastidious species 40 and 41, from stool specimens. to explore the possibility of using this cell line for the isolation of other viruses from clinical specimens, 293 cells were tested for their susceptibility to a variety of viruses including herpes simplex virus, parainfluenza viruses, respiratory syncytial virus, and the enter ...	1986	3009540
chromosome-specific subfamilies within human alphoid repetitive dna.	nucleotide sequence data of about 20 x 10(3) base-pairs of the human tandemly repeated alphoid dna are presented. the dna sequences were determined from 45 clones containing ecori fragments of alphoid dna isolated from total genomic dna. thirty of the clones contained a complete 340 base-pair dimer unit of the repeat. the remaining clones contained alphoid dna with fragment lengths of 311, 296, 232, 170 and 108 base-pairs. the sequences obtained were compared with an average alphoid dna sequence ...	1986	3009826
analysis of line-1 family sequences on a single monkey chromosome.	the structure of line-1 (l1ca) family members present on african green monkey chromosome cae-19 is compared with that of the entire set of l1ca sequences present in the monkey genome. the analysis involved annealing of cloned subsegments of monkey l1 family members to dna-blots containing restriction endonuclease digests of either total monkey liver dna or dna isolated from a monkey/mouse somatic cell hybrid carrying the single monkey chromosome. in addition, l1ca segments cloned from hybrid cel ...	1986	3012483
influence of dietary fiber on lipids and aortic composition of vervet monkeys.	a semipurified, cholesterol-free diet containing 40% carbohydrate can produce aortic sudanophilia or aortic atherosclerosis in vervet monkeys (cercopithecus aethiops pygerethrus) depending on the particular carbohydrate fed. four groups of vervet monkeys (three males and three females per group) were fed semipurified diets containing lactose. two of the groups were also fed 15% cellulose (c) or 15% cellulose plus 0.1% cholesterol (cc); the two other groups were fed 15% pectin (p) or 15% pectin p ...	1986	3014255
isolation of a t-lymphotropic retrovirus from naturally infected sooty mangabey monkeys (cercocebus atys).	healthy mangabey monkeys in a colony at the yerkes regional primate research center were found to be infected with a retrovirus related to human immunodeficiency virus (hiv). virus was isolated from peripheral blood cells of 14 of 15 randomly selected mangabeys. all virus-positive animals had antibodies to the mangabey virus at the time of virus isolation and, in a retrospective study, 82% of mangabey serum samples obtained in 1981 had antibodies to the virus. the newly isolated retrovirus is (i ...	1986	3014542
influence of acyclovir and bucyclovir on nucleotide pools in cells infected with herpes simplex virus type 1.	the effects of the acyclic guanosine analogs acyclovir (acv) and (r)-9-(3,4-dihydroxybutyl)guanine (bucyclovir, bcv) on the deoxyribonucleoside triphosphate (dntp) pools of herpes simplex type 1 (hsv-1)-infected african green monkey kidney (gmk) and human embryonic lung fibroblast (hl) cells were investigated. hsv-1 infection increased the dntp pools in both cell types compared with those in uninfected cells. mock-infected gmk cells showed a 10-fold-higher dttp concentration than comparable hl c ...	1986	3015006
kinetic properties of the sodium bicarbonate (carbonate) symport in monkey kidney epithelial cells (bsc-1). interactions between na+, hco-3, and ph.	bsc-1 kidney epithelial cells derived from the african green monkey are known to express a na+hco3- symport (jentsch, t. j., schill, b. s., schwartz, p., matthes, h., keller, s. k., and wiederholt, m. (1985) j. biol. chem. 260, 15554-15560). in the present work, 4,4-diisothiocyanostilbene-2,2'-disulfonic acid (dids)-sensitive 22na+ uptake into confluent monolayers of bsc-1 is measured in the presence of ouabain (10(-4) m) and amiloride (10(-3) m) to define the interactions between na+ and hco3- ...	1986	3015937
rescue of presumptive viral information from human cells by a helper oncovirus.	we have attempted to rescue presumptive human endogenous retrovirus(es) by using a competent animal oncovirus as a helper. human melanoma cells (line hmb2) were fused, using polyethylene glycol, with mouse nih-3t3 cells which had been infected and transformed by the harvey murine leukaemia and sarcoma virus complex (mlv and msv). the heteropolykaryons obtained were co-cultivated with fresh nih-3t3 cells; filtered (millipore 0.22 micron) medium from these was used to infect further nih-3t3 cells. ...	1986	3016151
human t-cell leukemia virus type i is a member of the african subtype of simian viruses (stlv).	the nucleotide sequences of the long terminal repeat (ltr) of simian retroviruses (stlv), which are closely related to human t-cell leukemia virus type i (htlv-i) were found to be of at least two subgroups: an asian subtype in macaques and an african subtype in african green monkeys and chimpanzee. the nucleotide sequence of htlv-i was found to be included within the divergence among stlv, but showed closer homology (95%) to african subtype stlv than to asian subtype stlv (90%).	1986	3002040
a field study of infection with human t-cell leukemia virus among african primates.	african non-human primates were surveyed seroepidemiologically for natural infection of human t-cell leukemia virus type i (atlv/htlv-i) or its closely related virus(es). materials from three genera (cercopithecus, papio, and theropithecus), four species (grivet monkey, anubis baboon, hamadryas baboon, and gelada), totalling 983 animals under natural conditions, were obtained in a field study in ethiopia. virus infection was determined by the indirect immunofluorescence test using htlv-i specifi ...	1986	2873501
antibodies to simian immunodeficiency virus in african green monkeys in africa in 1957-62.		1986	2874433
[effect of anxiolytics--buspirone and diazepam--on the prolactin, thyrotropin and cortisol content of the blood in the green monkey].	the influence of two anxiolytics--diazepam and buspirone--on prolactin, thyrotrophin and cortisol levels in green monkey (cercopithecus aethiops) plasma was studied 30 min following i/m injection. diazepam at 1 mg/kg decreased prolactin and cortisol levels by 30-50% compared to the control animals. buspirone at 2.5-10 mg/kg induced a 7-10-fold increase in prolactin level but did not change cortisol and thyrotrophin concentration. buspirone analog--mj 138-05 at 10 mg/kg produced a 2-3-fold increa ...	1986	2879573
survival and growth of fetal catecholamine neurons transplanted into primate brain.	dopamine and norepinephrine neuroblasts of the ventral mesencephalon, hypothalamus, and dorsolateral pons were transplanted from fetal african green monkeys into multiple brain sites in adult (host) african green monkeys. tissue was grafted from both early and late gestational age fetuses. immunohistochemical analysis, with antibodies to tyrosine hydroxylase, a marker of catecholamine-containing neurons, showed large numbers of transplanted catecholamine neurons in host cerebral cortex, corpus s ...	1986	2879615
hematologic abnormalities similar to the preleukemic state of adult t-cell leukemia in african green monkeys naturally infected with simian t-cell leukemia virus.	studies were made of the hematologic features of 23 adult african green monkeys (cercopithecus aethiops) naturally infected with simian t-cell leukemia virus (stlv), which is closely related to human t-cell leukemia virus type i. none of the stlv-infected monkeys showed any clinical signs, but their absolute lymphocyte count and percentage of atypical lymphocytes were significantly higher than those of uninfected monkeys. three stlv-infected monkeys had especially high percentages of atypical ly ...	1986	2880832
x-ray-induced base sequence damage in primate alphoid dna.	radiation-induced single-strand breaks were found throughout the 172 bp repeat units of african green monkey component alpha dna. two kinds of 3'-ends of 5'-32p-labeled restriction fragments were found, as previously described by others. after irradiation in vitro, the yield of single-strand breaks was 4 x 10(-5) breaks/nucleotide/gy, as determined by analyses in dna sequencing type gels. protection from x-ray damage was found when the dna received 150 gy in the presence of 2-mercaptoethanol. th ...	1986	3952274
movement of naegleria fowleri stimulated by mammalian cells in vitro.	naegleria fowleri amebae, but not those of n. australiensis, n. gruberi, or n. lovaniensis, demonstrated enhanced motility when placed in proximity to mammalian cells. amebae of nonpathogenic species of naegleria, however, were more motile in cell culture medium than the amebae of n. fowleri. the locomotory response of highly pathogenic mouse-passaged n. fowleri amebae to nerve cells was greater than axenically cultured amebae. the enhanced mobility elicited by whole nerve cells or disrupted ner ...	1986	3959003
micropuncture study of the superficial nephron of cercopithecus aethiops.	clearance and micropuncture experiments were performed on male cercopithecus monkeys weighing between 2 and 4 kg to evaluate the function of the superficial nephron. the mean glomerular filtration rate was 4.47 +/- 0.32 ml/min. the fraction of filtered sodium, potassium, calcium, magnesium, and phosphate remaining at the end of the accessible proximal tubule were 54 +/- 3, 64 +/- 4, 70 +/- 3, 79 +/- 5, and 39 +/- 5%, respectively. the concentration of magnesium and calcium in the tubule fluid ro ...	1986	3961264
conjugates of biogenic amines in ventriculocisternal perfusates of conscious african green monkeys.	to determine the importance of conjugation relative to alternate metabolic pathways for the inactivation of catecholamines and serotonin, free and conjugated norepinephrine, dopamine and serotonin, together with the acid metabolites homovanillic acid and 5-hydroxyindoleacetic acid, were measured in ventriculocisternal perfusions in awake african green monkeys. measurements were made of endogenous transmitters and their metabolites using high performance liquid chromatography with electrochemical ...	1986	3708364
synthesis and stability of nuclear matrix proteins in resting and serum-stimulated swiss 3t3 cells.	the major [35s]methionine-radiolabeled nuclear matrix proteins of mouse 3t3 cells were isolated, and most of these were found to be similar in molecular weight, charge, and protease fingerprint to the nuclear matrix proteins of african green monkey kidney cells, which are found tightly bound to simian virus 40 chromosomes. these nuclear matrix proteins were found to be synthesized in quiescent and serum-stimulated cells and to be turned over slowly during pulse-chase experiments. in contrast, a ...	1986	3711149
an improved hemagglutination test for study of canine parvovirus.	optimal conditions for hemagglutination (ha) by canine parvovirus (cpv) strains were investigated using several buffers. porcine erythrocytes often agglutinated spontaneously in phosphate-buffered salt solution, isotonic saline solution or barbitone-complement-fixation buffer. results were reproducible when borate-buffered saline (bbs) was used as the diluent for antigen, and "virus adjusting diluent" (vad), containing 0.15 m nacl and 0.3 m phosphate was used as the diluent for erythrocytes. hig ...	1986	3727364
activity of 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodouracil against simian varicella virus infections in african green monkeys.	the fluorinated pyrimidines 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau) and 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau) are highly effective inhibitors of herpesvirus infections in vitro and in vivo. this report is concerned with an evaluation of their activities in african green monkeys (cercopithecus aethiops) infected with simian varicella virus, a herpesvirus closely related to human varicella-zoster virus. oral or intravenous administration of ...	1986	3729332
cytoskeletal changes during adhesion and release: a comparison of human and nonhuman primate platelets.	the organization of cytoskeletal proteins in whole-mount adherent platelets from african green monkeys and normal human volunteers has been studied by sem, high vacuum electron microscopy (hvem) and conventional (120 kv) electron microscopy. we describe three distinct organizational zones, the central matrix, the trabecular zone and the peripheral web in spread platelets from both sources. the central matrix is an ill-defined superstructure of 80-100 a filaments of short length which enshrouded ...	1986	3738417
amitotic neuroblastoma cells used for neural implants in monkeys.	the potential utility of cultured neuroblastoma cells as donor tissue for neutral implants into the mammalian brain has been examined. cells from a human neuroblastoma cell line, imr-32, were labeled with [3h]thymidine and chemically rendered amitotic. these differentiated imr-32 cells were grafted into the hippocampi of five adult african green monkeys, and graft survival was evaluated for up to 270 days after transplantation. autoradiographically labeled grafted cells were identified in four a ...	1986	3749886
metabolism of t-2 mycotoxin by cultured cells.	t-2 mycotoxin is a small (i.e. mol. wt 466), non-protein toxin. we studied its metabolism in chinese hamster ovary (cho) cells, african green monkey kidney (vero) cells, human fibroblasts and mouse connective tissue cells (l-929). confluent cells were exposed to [3h]-t-2(0.01 micrograms/ml) for 1 hr at 37 degrees c. the toxin was removed, cells rinsed, and unlabeled culture media added for 4 hr (37 degrees c). cell monolayers were extracted and media and cell extracts were spotted on thin-layer ...	1986	3750347
expression and cell type--specific processing of human preproenkephalin with a vaccinia recombinant.	the posttranslational maturation of a complex precursor polyprotein, human proenkephalin, was assessed by infection of a wide spectrum of cell types with a recombinant vaccinia virus that expressed human proenkephalin. the infected cells rapidly produced both cellular and secreted met-enkephalin immunoreactivity. although each cell line could secrete intact proenkephalin, only a mouse pituitary line was capable of processing proenkephalin to mature enkephalin peptides. the quantity of intact pro ...	1986	3754979
a species comparison of low density lipoprotein heterogeneity in nonhuman primates fed atherogenic diets.	six male cynomolgus monkeys and five male african green monkeys were fed dietary cholesterol to induce hypercholesterolemia. the two groups studied had equivalent total plasma cholesterol concentrations. low density lipoproteins (ldl) were isolated from whole plasma by ultracentrifugation and separated from other lipoprotein classes by agarose column chromatography. ldl were further subfractionated by density gradient ultracentrifugation in a vti-50 vertical rotor. the material within five densi ...	1986	3760711
reproduction in the vervet monkey (cercopithecus aethiops): endometrial oestrogen and progestin receptor dynamics during normal and prolonged menstrual cycles.	sixteen individually caged adult female vervet monkeys (cercopithecus aethiops), whose reproductive parameters had been studied for 5 years, were hysterectomized/ovariectomized during three reproductive states; i.e. the late follicular (15.4 +/- 4.7 (s.d.) days) and luteal (27.8 +/- 4.7 days) phases of the normal cycle (20-50 days), and during prolonged intermenstrual intervals (pii; 99.0 +/- 2.5 days since the previous menses). these latter animals showed characteristics of both follicular and ...	1986	3760741