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detection and characterization of antimicrobial resistance and putative virulence genes in aeromonas veronii biovar sobria isolated from gilthead sea bream (sparus aurata l.).the purposes of this study were to investigate the antimicrobial resistance of aeromonas veronii biovar sobria isolated from gilthead sea bream and to characterize the virulence-implicated genes. fish samples (n=365) were collected from wholesale and retail markets in aljouf, saudi arabia between 2013 and 2014. a total of 45 a. veronii biovar sobria isolates (12.3%) from those samples were tested for resistance to a range of antimicrobial agents. all strains exhibited 100% resistances to nalidix ...201526218714
draft genome sequence of aliivibrio fischeri strain 5lc, a bacterium retrieved from gilthead sea bream (sparus aurata) larvae reared in aquaculture.to shed light on the putative host-mediated lifestyle of the quintessential marine symbiont aliivibrio fischeri, and on the symbiosis versus potentially pathogenic features of bacteria associated with farmed fish, we report the draft genome sequence of a. fischeri strain 5lc, a bacterium retrieved from gilthead sea bream (sparus aurata) larvae.201526044435
preliminary investigation on the use of allyi isothiocyanate to increase the shelf-life of gilthead sea bream (sparus aurata) fillets.the aim of this work is to evaluate the activity of allyl isothiocyanate (aitc) against fish spoilage bacteria (specific spoilage organisms; ssos) as well as its possible use in gilthead sea bream (sparus aurata) fillets to extend their shelf-life. in this regard, in vitro tests are carried out in order to evaluate the inhibitory activity of aitc and its vapours on several strains of ssos. the aitc effect on the shelf-life of sea bream fillets was made by putting them in plastic trays hermetical ...201527800402
nodavirus colonizes and replicates in the testis of gilthead seabream and european sea bass modulating its immune and reproductive functions.viruses are threatening pathogens for fish aquaculture. some of them are transmitted through gonad fluids or gametes as occurs with nervous necrosis virus (nnv). in order to be transmitted through the gonad, the virus should colonize and replicate inside some cell types of this tissue and avoid the subsequent immune response locally. however, whether nnv colonizes the gonad, the cell types that are infected, and how the immune response in the gonad is regulated has never been studied. we have de ...201526691348
high diversity of skin-associated bacterial communities of marine fishes is promoted by their high variability among body parts, individuals and species.animal-associated microbiotas form complex communities, which are suspected to play crucial functions for their host fitness. however, the biodiversity of these communities, including their differences between host species and individuals, has been scarcely studied, especially in case of skin-associated communities. in addition, the intraindividual variability (i.e. between body parts) has never been assessed to date. the objective of this study was to characterize skin bacterial communities of ...201526048284
rapid extraction and reverse phase-liquid chromatographic separation of mercury(ii) and methylmercury in fish samples with inductively coupled plasma mass spectrometric detection applying oxygen addition into plasma.a simple and sensitive procedure was developed for extraction and speciation of mercury in fish. species separation was accomplished with reverse phase-high performance liquid chromatography (hplc) hyphenated to inductively coupled plasma mass spectrometry (icp-ms). oxygen addition into plasma allowed use of organic-rich mobile phase, achieving species separation in 4 min. mercury species extraction was achieved by microwave exposure for 2 min at mild conditions (60°c, ph 2.0), avoiding necessit ...201525872437
trace metals in tissues of the six most common fish species in the black sea, turkey.concentrations of mn, fe, co, ni, as, se, cd, ag and pb in scale, skin, muscle, gills, liver and the gonads of gilthead seabream (sparus aurata), european seabass (dicentrarchus labrax), black sea salmon (salmo trutta labrax), horse mackerel (trachurus trachurus), red mullet (mullus barbatus) and whiting (merlangius merlangius euxinus) from the black sea, in turkey, were investigated. elemental analyses were performed with inductively coupled plasma-mass spectrometry after sample preparation by ...201525082436
description and comparative study of physico-chemical parameters of the teleost fish skin mucus.the study of mucosal surfaces, and in particular the fish skin and its secreted mucus, has been of great interest recently among immunologists. measurement of the viscosity and other physico-chemical parameters (protein concentration, ph, conductivity, redox potential, osmolality and density) of the skin mucus can help to understand its biological functions. we have used five marine species of teleost: gilthead seabream (sparus aurata l.), european sea bass (dicentrarchus labrax l.), shi drum (u ...201526484392
molecular evidence for the existence of species complexes within macvicaria gibson & bray, 1982 (digenea: opecoelidae) in the western mediterranean, with descriptions of two new species.morphological and molecular characterisation of specimens of macvicaria spp. (digenea: opecoelidae) from five species of mediterranean sparids (teleostei: sparidae) sampled in the bizerte lagoon and the bay of bizerte (tunisia) revealed the presence of two species complexes designated as "obovata" and "crassigula" groups. detailed comparative morphological and phylogenetic analyses revealed that two of the specimen sets, one from each complex, represent species new to science. m. maamouriae n. s ...201526063299
ultrastructure and phylogeny of ceratomyxa sp. (myxosporea), a parasite infecting sparus aurata (teleostei) in a portuguese fish farm. 201526227725
ultrastructure and phylogeny of ceratomyxa auratae n. sp. (myxosporea: ceratomyxidae), a parasite infecting the gilthead seabream sparus aurata (teleostei: sparidae).a new myxosporean parasite is described from the gall bladder of the gilthead seabream sparus aurata in a southern portuguese fish farm, with basis on light and transmission electron microscopy, as well as in molecular procedures. in the bile, young and mature mono- to disporic plasmodia were elliptical and presented smooth surface membranes. crescent-shaped myxospores measured 6.7±0.7 (5.3-7.6) μm in length and 27.0±3.0 (19.7-31.2) μm in thickness. the myxospore wall was constituted by two symm ...201525892564
using a concentrate of phenols obtained from olive vegetation water to preserve chilled food: two case studies.phenols are plant metabolites characterised by several interesting bioactive properties such as antioxidant and bactericidal activities. in this study the application of a phenols concentrate (pc) from olive vegetation water to two different fresh products - gilt-head seabream (sparus aurata) and chicken breast - was described. products were treated in a bath of pc (22 g/l; chicken breast) or sprayed with two different solutions (l1:0.75 and l2:1.5 mg/ml; seabream) and then stored under refriger ...201627800444
the bromodomain testis-specific gene (brdt) characterization and expression in gilthead seabream, sparus aurata, and european seabass, dicentrarchus labrax.multiple genes and transcription factors are involved in regulation and control of the complex process of sex determination and differentiation of fish species. also more, several hormonal factors and some environmental conditions can also be adequate spawning strategies and stimuli for inducing reproduction of fish species. brdt gene belongs to the bromodomain-extraterminal domain (bet) family of transcriptional coregulators. in mammals, brdt gene is almost exclusively expressed in testis. furt ...201627349318
importance of prebiotics in aquaculture as immunostimulants. effects on immune system of sparus aurata and dicentrarchus labrax.infectious diseases in fish represent a major problem for the aquaculture field as they produce extensive damages and loss. over the last few years, with increased development of the aquaculture industry, different methods have been used to contrast these pathologies. common interest has led to the use of components (as additives in diets) that could contrast diseases without causing any negative impact on the environment. these components are represented by prebiotics, probiotics, and plant ext ...201627074444
in vitro effects of metals on isolated head-kidney and blood leucocytes of the teleost fish sparus aurata l. and dicentrarchus labrax l.the in vitro use of fish leucocytes to test the toxicity of aquatic pollutants, and particularly the immutoxicological effects, could be a valuable alternative to fish bioassays but has received little attention. in this study, head-kidney and peripheral blood leucocytes (hkls and pbls, respectively) from gilthead seabream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.) specimens were exposed to cd, mehg (methylmercury), pb or as for 24 h being evaluated the resulting cytotoxi ...201627041665
stress and humoral innate immune response of gilthead seabream sparus aurata cultured in sea cages.innate and acquired immune responses of gilthead seabream sparus aurata was studied under normal culture and short-term stressful conditions for 18 months in offshore sea cages in alghero bay, italy. every 45 d, 50 fish were sampled and divided into two groups: fish in the first group (normal culture conditions) were bled after harvesting; fish in the second group were put into a tank under stressful conditions (crowding and confinement) and bled after 2 h. innate humoral immunity, such as compl ...201627485027
molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells.toll-like receptor 2 (tlr2) has been shown to play a crucial role in the host defense of pathogenic microbes in innate immunity. in this study, the full-length cdna of tlr2 in silvery pomfret (pampus argenteus) was cloned by homology cloning and the rapid amplification of cdna ends (race) technique. the complete cdna sequence of tlr2 was 2932 bp, containing an open reading frame (orf) of 2469 bp encoding 822 amino acids. a multiple alignment analysis of the silvery pomfret tlr2 protein-coding se ...201627574826
preliminary study on the in vitro and in vivo effects of asparagopsis taxiformis bioactive phycoderivates on teleosts.several compounds from marine organisms have been studied for their potential use in aquaculture. among the red algae, asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. depending on the seasonal period of sampling, ethanol extracts of a. taxiformis exhibited signi ...201627826246
transient up- and down-regulation of expression of myosin light chain 2 and myostatin mrna mark the changes from stratified hyperplasia to muscle fiber hypertrophy in larvae of gilthead sea bream (sparus aurata l.).hyperplasia and hypertrophy are the two mechanisms by which muscle develops and grows. we study these two mechanisms, during the early development of white muscle in sparus aurata, by means of histology and the expression of structural and regulatory genes. a clear stage of stratified hyperplasia was identified early in the development of gilthead sea bream but ceased by 35 dph when hypertrophy took over. mosaic recruitment of new white fibers began as soon as 60 dph. the genes mlc2a and mlc2b w ...201626246399
labeling and label free shotgun proteomics approaches to characterize muscle tissue from farmed and wild gilthead sea bream (sparus aurata).the proteome characterization of fish muscle tissues, together with the relative expression of each individual protein, provides knowledge on the biochemical response of the organisms and allows to assess the effect of different types of feeding, growth site and nutritional quality of the investigated species. this type of study is usually performed by gel-based proteomics approaches, however shotgun proteomics can serve as well, reducing analysis time and improving sample high-throughput. in th ...201626233254
assessment of freshness and freeze-thawing of sea bream fillets (sparus aurata) by a cytosolic enzyme: lactate dehydrogenase.the evaluation of freshness and freeze-thawing of fish fillets was carried out by assessment of autolysis of cells using a cytosolic enzyme lactate dehydrogenase. autolysis plays an important role in spoilage of fish and postmortem changes in fish tissue are due to the breakdown of the cellular structures and release of cytoplasmic contents. the outflow of a cytosolic enzyme, lactate dehydrogenase, was studied in sea bream fillets and the sparus aurata fibroblasts (saf-1) cell-line during an 8da ...201627211667
dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (sparus aurata) juveniles.a study was undertaken to evaluate dietary glutamine supplementation effects on gilthead sea bream performance, intestinal nutrient absorption capacity, hepatic and intestinal glutamine metabolism and oxidative status. for that purpose gilthead sea bream juveniles (mean weight 13.0g) were fed four isolipidic (18% lipid) and isonitrogenous (43% protein) diets supplemented with 0, 0.5, 1 and 2% glutamine for 6weeks. fish performance, body composition and intestinal nutrient absorption capacity wer ...201626424608
jellyfish stings trigger gill disorders and increased mortality in farmed sparus aurata (linnaeus, 1758) in the mediterranean sea.jellyfish are of particular concern for marine finfish aquaculture. in recent years repeated mass mortality episodes of farmed fish were caused by blooms of gelatinous cnidarian stingers, as a consequence of a wide range of hemolytic, cytotoxic, and neurotoxic properties of associated cnidocytes venoms. the mauve stinger jellyfish pelagia noctiluca (scyphozoa) has been identified as direct causative agent for several documented fish mortality events both in northern europe and the mediterranean ...201627100175
energy metabolism of hyperthyroid gilthead sea bream sparus aurata l.thyroid hormones, in particular 3,5,3'-triiodothyronine or t3, are involved in multiple physiological processes in mammals such as protein, fat and carbohydrate metabolism. however, the metabolic actions of t3 in fish are still not fully elucidated. we therefore tested the effects of t3 on sparus aurata energy metabolism and osmoregulatory system, a hyperthyroid-induced model that was chosen. fish were implanted with coconut oil depots (containing 0, 2.5, 5.0 and 10.0μg t3/g body weight) and sam ...201626419695
effects of sustained exercise on gh-igfs axis in gilthead sea bream (sparus aurata).the endocrine system regulates growth mainly through the growth hormone (gh)/insulin-like growth factors (igfs) axis and, although exercise promotes growth, little is known about its modulation of these factors. the aim of this work was to characterize the effects of 5 wk of moderate sustained swimming on the gh-igfs axis in gilthead sea bream fingerlings. plasma igf-i/gh ratio and tissue gene expression of total igf-i and three splice variants, igf-ii, three igf binding proteins, two gh recepto ...201626661095
igf-i and igf-ii effects on local igf system and signaling pathways in gilthead sea bream (sparus aurata) cultured myocytes.the insulin-like growth factors (igfs) have a fundamental role in a vast range of functions acting through a tyrosine-kinase receptor (igf-ir). igfs in muscle can affect the expression of components of the local igf system, myogenic regulatory factors (mrfs), proliferating (proliferating cell nuclear antigen, pcna) or differentiating molecules (myosin heavy chain, mhc) and, lead to the activation of different signaling pathways. the response of all these genes to igfs incubation at two different ...201626602376
vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (sparus aurata, linnaeus 1758).this study examined the effects of boat noise pollution on the stress indices of gilthead sea bream (sparus aurata, linnaeus 1758). to assess the stress response in these fish, biometric values and plasma parameters such as acth, cortisol, glucose, lactate, haematocrit, hsp70, total protein, cholesterol, triglycerides and osmolarity were analysed. after acclimatization of the animals, the experiment was carried out in a tank fitted with underwater speakers where the fish were exposed to sound tr ...201626581747
insulin-like growth factor 1 (igf-1) regulates prolactin, growth hormone, and igf-1 receptor expression in the pituitary gland of the gilthead sea bream sparus aurata.the role of insulin-like growth factor 1 (igf-1) on regulation of growth hormone (gh) and prolactin (prl) as well as the possible involvement of igf-1 receptor subtype a (igf-1ra) mrna was assessed in juvenile specimens of sparus aurata. igf-1ra was successfully cloned, and active receptor domains were localized in its mrna precursor. also, phylogenetic analysis of the protein sequence indicated a closer proximity to igf-1ra isoform found in zebrafish and other teleosts, than to the isoform igf- ...201626486515
predicting gilthead sea bream (sparus aurata) freshness by a novel combined technique of 3d imaging and sw-nir spectral analysis.a technique that combines the spatial resolution of a 3d structured-light (sl) imaging system with the spectral analysis of a hyperspectral short-wave near infrared system was developed for freshness predictions of gilthead sea bream on the first storage days (days 0-6). this novel approach allows the hyperspectral analysis of very specific fish areas, which provides more information for freshness estimations. the sl system obtains a 3d reconstruction of fish, and an automatic method locates gil ...201627775556
performance of maximum likelihood mixture models to estimate nursery habitat contributions to fish stocks: a case study on sea bream sparus aurata.mixture models (mm) can be used to describe mixed stocks considering three sets of parameters: the total number of contributing sources, their chemical baseline signatures and their mixing proportions. when all nursery sources have been previously identified and sampled for juvenile fish to produce baseline nursery-signatures, mixing proportions are the only unknown set of parameters to be estimated from the mixed-stock data. otherwise, the number of sources, as well as some/all nursery-signatur ...201627761305
having a blast: searchable transcriptome resources for the gilthead sea bream and the european sea bass.the gilthead sea bream (sparus aurata) and the european sea bass (dicentrarchus labrax) are the most important aquaculture species in the mediterranean sea and since the last decade it has been seen an exponential increase in their available molecular resources. in order to improve accessibility to transcriptome resources, expressed sequence tags (ests), mrna sequences and raw read sequences were assembled and deposited in blast queryable databases. the publicly available sea bream and sea bass ...201627742405
adipogenic gene expression in gilthead sea bream mesenchymal stem cells from different origin.during the last decades, adipogenesis has become an emerging field of study in aquaculture due to the relevance of the adipose tissue in many physiological processes and its connection with the endocrine system. in this sense, recent studies have translated into the establishment of preadipocyte culture models from several fish species, sometimes lacking information on the mrna levels of adipogenic genes. thus, the aim of this study was to determine the gene expression profile of gilthead sea br ...201627597840
replacement of marine fish oil with de novo omega-3 oils from transgenic camelina sativa in feeds for gilthead sea bream (sparus aurata l.).omega-3 (n-3) long-chain polyunsaturated fatty acids (lc-pufa) are essential components of the diet of all vertebrates. the major dietary source of n-3 lc-pufa for humans has been fish and seafood but, paradoxically, farmed fish are also reliant on marine fisheries for fish meal and fish oil (fo), traditionally major ingredients of aquafeeds. currently, the only sustainable alternatives to fo are vegetable oils, which are rich in c18 pufa, but devoid of the eicosapentaenoic (epa) and docosahexae ...201627590240
activity of r(+) limonene on the maximum growth rate of fish spoilage organisms and related effects on shelf-life prolongation of fresh gilthead sea bream fillets.r(+)limonene (lmn) is the major aromatic compound in essential oils obtained from oranges, grapefruits, and lemons. the improvement of preservation techniques to reduce the growth and activity of spoilage microorganisms in foods is crucial to increase their shelf life and to reduce the losses due to spoilage. the aim of this work is to evaluate the effect of lmn on the shelf life of fish fillets. its effectiveness was preliminarily investigated in vitro against 60 strains of specific spoilage or ...201627552348
transcriptomic changes in relation to early-life events in the gilthead sea bream (sparus aurata).teleosts are exposed to a broad range of external stimuli, which may be either of acute or chronic nature. the larval phase of certain fish species offer a unique opportunity to study the interactions between genes and environmental factors during early life. the present study investigates the effects of early-life events, applied at different time points of early ontogeny (first feeding, flexion and development of all fins; phase 1) as well as on the subsequent juvenile stage after the applicat ...201627461489
dietary butyrate helps to restore the intestinal status of a marine teleost (sparus aurata) fed extreme diets low in fish meal and fish oil.there is a constant need to find feed additives that improve health and nutrition of farmed fish and lessen the intestinal inflammation induced by plant-based ingredients. the objective of this study was to evaluate the effects of adding an organic acid salt to alleviate some of the detrimental effects of extreme plant-ingredient substitution of fish meal (fm) and fish oil (fo) in gilthead sea bream diet. three experiments were conducted. in a first trial (t1), the best dose (0.4%) of sodium but ...201627898676
nadph production, a growth marker, is stimulated by maslinic acid in gilthead sea bream by increased nadp-idh and me expression.nadph plays a central role in reductive biosynthesis of membrane lipids, maintenance of cell integrity, protein synthesis and redox balance maintenance. hence, nadph is involved in the growth and proliferation processes. in addition, it has been shown that changes in nutritional conditions produced changes in nadph levels and growth rate. maslinic acid (ma), a pentacyclic triterpene of natural origin, is able to stimulate nadph production, through regulation of the two oxidative phase dehydrogen ...201627178358
influence of time, storage temperature and freeze/thaw cycles on the activity of digestive enzymes from gilthead sea bream (sparus aurata).in this study, we tested the effects of long-term storage (2 years) at -20 °c and short-term storage (several hours) in ice and freeze/thaw cycles on the activities of pancreatic, gastric and intestinal (brush border and cytosolic) digestive enzymes in a teleost fish species. the results revealed a significant lose in activity of pancreatic (trypsin, chymotrypsin, total alkaline proteases and α-amylase) and intestinal cytosolic (leucine-alanine peptidase) enzymes between 140 and 270 days of stor ...201627083435
ocean warming alters cellular metabolism and induces mortality in fish early life stages: a proteomic approach.climate change has pervasive effects on marine ecosystems, altering biodiversity patterns, abundance and distribution of species, biological interactions, phenology, and organisms' physiology, performance and fitness. fish early life stages have narrow thermal windows and are thus more vulnerable to further changes in water temperature. the aim of this study was to address the sensitivity and underlying molecular changes of larvae of a key fisheries species, the sea bream sparus aurata, towards ...201627062348
dietary lipid and carbohydrate interactions: implications on lipid and glucose absorption, transport in gilthead sea bream (sparus aurata) juveniles.a digestibility trial was performed with gilthead sea bream juveniles (ibw = 72 g) fed four diets differing in lipid source (fish oil, fo; or a blend of vegetable oil, vo) and starch content (0 %, ch-; or 20 %, ch+) to evaluate the potential interactive effects between carbohydrates and vo on the processes involved in digestion, absorption and transport of lipids and glucose. in fish fed vo diets a decrease in lipid digestibility and in cholesterol (c), high density lipoprotein(hdl)-c and low de ...201627023202
cytotoxicity and alterations at transcriptional level caused by metals on fish erythrocytes in vitro.the in vitro use of fish erythrocytes to test the toxicity of aquatic pollutants could be a valuable alternative to fish bioassays but has received little attention. in this study, erythrocytes from marine gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.) specimens were exposed for 24 h to cd, hg, pb and as and the resulting cytotoxicity was evaluated. exposure to metals produced a dose-dependent reduction in the viability, and mercury showed the highest toxic ...201626976014
molecular endocrine changes of gh/igf1 axis in gilthead sea bream (sparus aurata l.) exposed to different environmental salinities during larvae to post-larvae stages.the influence of acclimation of the euryhaline gilthead sea bream (sparus aurata) larvae/post-larvae to brackish water on growth, energetic contents, and mrna levels of selected hormones and growth-regulating hypothalamic neurohormones was assessed. specimens from 49 days post-hatching were acclimated during 28 days to two different environmental salinities: 38 and 20 psu (as brackish water). both groups were then transferred to 38 psu and acclimated for an additional week. early juveniles were ...201626947706
potential use of high levels of vegetal proteins in diets for market-sized gilthead sea bream (sparus aurata).the effect of partial or total dietary substitution of fishmeal (fm) by vegetal protein sources on growth and feed efficiency was carried out in on-growing gilthead sea bream (mean initial weight 131 g). the control diet (fm 100) contained fm as the primary protein source, while in diets fm 25 and fm 0 the fm protein was replaced at 75% and 100%, respectively, by a vegetable protein mixture consisting of wheat gluten, soybean meal, rapeseed meal and crystalline amino acids. diets fm 25 and fm 0 ...201626880503
unraveling the tissue-specific gene signatures of gilthead sea bream (sparus aurata l.) after hyper- and hypo-osmotic challenges.a custom microarray was used for the transcriptomic profiling of liver, gills and hypothalamus in response to hypo- (38‰ → 5‰) or hyper- (38‰ → 55‰) osmotic challenges (7 days after salinity transfer) in gilthead sea bream (sparus aurata) juveniles. the total number of differentially expressed genes was 777. among them, 341 and 310 were differentially expressed in liver after hypo- and hyper-osmotic challenges, respectively. the magnitude of changes was lower in gills and hypothalamus with aroun ...201626828928
lysine and leucine deficiencies affect myocytes development and igf signaling in gilthead sea bream (sparus aurata).optimizing aquaculture production requires better knowledge of growth regulation and improvement in diet formulation. a great effort has been made to replace fish meal for plant protein sources in aquafeeds, making necessary the supplementation of such diets with crystalline amino acids (aa) to cover the nutritional requirements of each species. lysine and leucine are limiting essential aa in fish, and it has been demonstrated that supplementation with them improves growth in different species. ...201626808650
morphological characterization of the oral cavity of the gilthead seabream (sparus aurata) with emphasis on the teeth-age adaptation.gilthead sea bream with different age groups that collected from seawater fisheries, ismailia governorate, egypt, were examined by gross anatomy and scanning electron microscopy to assess the morphological characteristics of the oral cavity. teeth patterns showed that the gilthead sea bream is adapted to the feeding pattern according to age development, as it modified from spiny form teeth in young fishes to obelisk-like teeth and flat dome-like teeth in growing fishes, with differentiation of t ...201626791335
dna damage and differential gene expression associated with physical stress in gilthead seabream (sparus aurata).fish stress may result in inhibition of reproduction, development and growth. thus, appropriate indices should be developed to accurately define the physiological plasticity of fish, in terms of coping with stress. sea bream individuals were subjected to physical stress (fasting and confinement). dna fragmentation of liver cells was assessed, in addition to gene expression of selected genes and plasma cortisol levels determination. stress response was characterized with significant temporal alte ...201627401265
partitioning the metabolic scope: the importance of anaerobic metabolism and implications for the oxygen- and capacity-limited thermal tolerance (ocltt) hypothesis.ongoing climate change is predicted to affect the distribution and abundance of aquatic ectotherms owing to increasing constraints on organismal physiology, in particular involving the metabolic scope (ms) available for performance and fitness. the oxygen- and capacity-limited thermal tolerance (ocltt) hypothesis prescribes ms as an overarching benchmark for fitness-related performance and assumes that any anaerobic contribution within the ms is insignificant. the ms is typically derived from re ...201627293766
fast real time pcr for control of intra-species recycling in aquaculture feed, focused to the most relevant fish species farmed in europe.recent regulations in animal feed composition prohibit intra-species recycling, the recycling of one given animal species to the same species, in order to avoid potential safety risks to human and animal health. these regulations have generated the need of their control in aquaculture by effective and specific analytical techniques. to date, most studies of species identification and detection in feedstuffs are focused on land species, but few studies are focused on species composition in fish f ...201626988512
determination of sulfadiazine, trimethoprim, and n(4) -acetyl-sulfadiazine in fish muscle plus skin by liquid chromatography-mass spectrometry. withdrawal-time calculation after in-feed administration in gilthead sea bream (sparus aurata l.) fed two different diets.this study presents a depletion study for sulfadiazine and trimethoprim in muscle plus skin of gilthead sea bream (sparus aurata l.). n(4) -acetyl-sulfadiazine, the main metabolite of sulfadiazine (sdz), was also examined. the fish were held in seawater at a temperature of 24-26 °c. sdz and trimethoprim (tmp) were administered orally with medicated feed for five consecutive days at daily doses of 25 mg sdz and 5 mg tmp per kg of fish body weight per day. two different diets, fish oil- and plant ...201626987772
comparison of the transcriptional responses of skeletal muscle and bone to a flooding dose of leucine in the gilthead sea bream (sparus aurata).skeletal muscle, cartilage and bone must function in a co-ordinated fashion during locomotion and growth. in the present study on the gilthead sea bream (sparus aurata) we tested the hypothesis that muscle and bone differ in their responsiveness to stimuli eliciting fast growth, providing a potential mechanism for generating the skeletal deformities observed in aquaculture. to investigate transcription regulation in skeletal muscle and bone we stimulated protein synthesis using a flooding dose o ...201626921774
characterization data of gilthead sea bream (sparus aurata) igf-i receptors (igf-ira/rb).in this data article we describe the coding sequence of two igf-ir paralogues (igf-ira and igf-irb) obtained from gilthead sea bream embryos. the putative protein architecture (domains and other important motifs) was determined and, amino acid sequences alignment and phylogenetic analysis of both receptors together with igf-ir orthologues from different vertebrates was performed. additionally, a semi-quantitative conventional pcr was done to analyze the mrna expression of both receptors in diffe ...201626904713
effect of short chain fructooligosaccharides (scfos) on immunological status and gut microbiota of gilthead sea bream (sparus aurata) reared at two temperatures.the effects of dietary short chain fructooligosaccharides (scfos) incorporation on hematology, fish immune status, gut microbiota composition, digestive enzymes activities, and gut morphology, was evaluated in gilthead sea bream (sparus aurata) juveniles reared at 18 °c and 25 °c. for that purpose, fish with 32 g were fed diets including 0, 0.1, 0.25 and 0.5% scfos during 8 weeks. overall, scfos had only minor effects on gilthead sea bream immune status. lymphocytes decreased in fish fed the 0.1 ...201626721230
gilthead seabream (sparus aurata) mx gene promoters respond differentially to ipnv and vhsv infections in rtg-2 cells.the understanding of virus-host interactions relies on the knowledge of the regulatory mechanisms of the type i interferon (ifn i)-stimulated genes (isgs). among isgs, those coding mx proteins play a main role due to their direct antiviral activity. the study of these genes in gilthead seabream is interesting, since this species displays high natural resistance to viral diseases, being asymptomatic carrier of infectious pancreatic necrosis virus (ipnv) and viral haemorrhagic septicaemia virus (v ...201626964720
differential modulation of igt and igm upon parasitic, bacterial, viral, and dietary challenges in a perciform fish.three different immunoglobulin (ig) isotypes can be found in teleost fish, igm, igd, and the teleost-specific igt. igm is considered to have a systemic activity, and igt is attributed a mucosal role, similar to mammalian iga. in this study, the complete sequence of gilthead sea bream igm and igt in their membrane (m) and soluble (s) forms are described for the first time in a perciform fish. their constitutive gene expression is analyzed in different tissues, and their regulation upon viral, bac ...201628082977
genomic prediction of resistance to pasteurellosis in gilthead sea bream (sparus aurata) using 2b-rad sequencing.gilthead sea bream (sparus aurata) is a species of paramount importance to the mediterranean aquaculture industry, with an annual production exceeding 140,000 metric tonnes. pasteurellosis due to the gram-negative bacterium photobacterium damselae subsp. piscicida (phdp) causes significant mortality, especially during larval and juvenile stages, and poses a serious threat to bream production. selective breeding for improved resistance to pasteurellosis is a promising avenue for disease control, ...201627652890
transcription of histones h1 and h2b is regulated by several immune stimuli in gilthead seabream and european sea bass.histones (h1 to h4) are the primary proteins which mediate the folding of dna into chromatin; however, and in addition to this function, histones have been also related to antimicrobial peptides (amps) activity in vertebrates, in fact, mammalian h1 is mobilized as part as the anti-viral immune response. in fish, histones with amp activity have been isolated and characterized mainly from skin and gonads. one of most threatening pathogens for wild and cultured fish species nowadays is nodavirus (n ...201627523279
pathogenesis of photobacterium damselae subspecies infections in sea bass and sea bream.photobacterium damselae is a gram negative bacterium causes photobacteriosis, a worldwide septicemic disease in aquaculture including sea bass (dicentrarchus labrax) and sea bream (sparus aurata). the pathogenicity of bacterial subspecies and the disease pathological changes in natural and experimental infections have thus far yielded inconsistency of effective preventive measures. this study aimed to represent a comprehensive analysis of the potential pathogenic capacities of the two subspecies ...201627497892
in vitro cytokine profile revealed differences from dorsal and ventral skin susceptibility to pathogen-probiotic interaction in gilthead seabream.skin is the first barrier of defense on fish, which is crucial to protection against different stressors, including pathogens. skin samples obtained from dorsal and ventral part of sparus aurata specimens were incubated with photobacterium damselae subsp. piscicida (a pathogen for this fish species), with shewanella putrefaciens pdp11 (a probiotic bacteria isolated from healthy gilthead seabream skin) or with both bacteria. the gene expression profile of nine cytokines (il1b, tnfa, il6, il7, il8 ...201627422755
characterization of the gilthead seabream (sparus aurata l.) immune response under a natural lymphocystis disease virus outbreak.lymphocystis or lymphocystis disease virus (lcdv) is distributed worldwide and affects many fresh and marine water fish species. lcdv is commonly found in aquaria fish species but also in farmed fish species, among them the gilthead seabream (sparus aurata l.). the immune status of gilthead seabream (s. aurata) specimens under a natural outbreak of lcdv was studied. the replication of the virus was demonstrated in infected fish, but not in control fish. the results showed decreased total serum i ...201627133966
evaluation of an integrated cell culture rt-pcr assay to detect and quantify infectious lymphocystis disease virus.the lymphocystis disease virus (lcdv), a member of the iridoviridae family, infects a wide range of fish species including gilthead seabream (sparus aurata l.), the most important species cultured in the mediterranean. lcdv is difficult to propagate in cell culture and does not produce clear and consistent cytopathic effects (cpe), especially in samples collected from subclinically infected fish. an integrated cell culture reverse transcription-polymerase chain reaction (icc-rt-pcr) assay, follo ...201627756548
development of a convenience and safety chilled sous vide fish dish: diversification of aquacultural products.the dynamic expansion of the ready-to-eat seabream sector in its adaptation to new lifestyles has led to the search for new presentation formats in seabream (sparus aurata). green sauce (olive oil, wine vinegar, garlic, fresh parsley, black pepper, basil and salt) and 60 ℃ of cooking temperature were chosen by the panellists for the sous vide cooking process. seabream fillet and sauce were packaged in polypropylene trays, cooked, chilled and stored at 2 ℃. microbiological (total viable counts,en ...201625941212
morphology and molecular analysis of life-cycle stages of proctoeces maculatus (looss, 1901) (digenea: fellodistomidae) in the bizerte lagoon, tunisia.the life cycle of proctoeces maculatus (looss, 1901) (digenea, fellodistomidae) was studied in bizerte lagoon (tunisia). three sequential hosts appear to be involved: the mediterranean mussel mytilus galloprovincialis lamarck, 1819 (mytilidae) as the first intermediate host; the polychaete sabella pavonina savigny, 1822 (sabellidae), as the second intermediate host; and fishes (lithognathus mormyrus (linnaeus, 1758) (sparidae), trachinotus ovatus (linnaeus, 1758) (carangidae) and sparus aurata l ...201626694018
concurrence of iridovirus, polyomavirus, and a unique member of a new group of fish papillomaviruses in lymphocystis disease-affected gilthead sea bream.lymphocystis disease is a geographically widespread disease affecting more than 150 different species of marine and freshwater fish. the disease, provoked by the iridovirus lymphocystis disease virus (lcdv), is characterized by the appearance of papillomalike lesions on the skin of affected animals that usually self-resolve over time. development of the disease is usually associated with several environmental factors and, more frequently, with stress conditions provoked by the intensive culture ...201627440877
immunostimulatory effects of prolactin on tlr1 and tlr5m in shk-1 cells infected with piscirickettsia salmonis.the innate immune system is the first line of defense against infection by pathogens. it consists of various elements, including toll-like receptors (tlrs), which recognize molecular patterns associated with pathogens and trigger the immune response, through activation of important transcription factors such as nf-κb, which are usually found sequestered in the cytoplasm by iκbα until it receives the release signal. piscirickettsia salmonis causes piscirickettsiosis or salmonid rickettsial septic ...201627025311
application of a new real-time polymerase chain reaction assay for surveillance studies of lymphocystis disease virus in farmed gilthead seabream.lymphocystis disease (lcd) is the main viral infection reported to affect cultured gilthead seabream (sparus aurata) in europe. the existence of subclinical lymphocystis disease virus (lcdv) infection in this fish species has been recognised by using polymerase chain reaction (pcr)-based methods. nevertheless, these methods do not provide quantitative results that can be useful in epidemiological and pathological studies. moreover, carrier fish have been involved in viral transmission, therefore ...201627048523
effects of shewanella putrefaciens on innate immunity and cytokine expression profile upon high stocking density of gilthead seabream specimens.high stocking density increases the number of emerging diseases triggering economic losses worldwide. probiotics provide an effective and natural solution for preventing some diseases through an improvement of innate immune system among others. in the present work dietary administration of the probiotic shewanella putrefaciens (known as pdp11) was evaluated under stress by high stocking density after 2 and 4 weeks of administration to gilthead seabream (sparus aurata) specimens. results showed a ...201626879495
enrichment of gilthead seabream (sparus aurata l.) diet with palm fruit extracts and probiotics: effects on skin mucosal immunity.fish skin mucus contains numerous immune substances still poorly studied. to date, there are no studies regarding the possible influence of dietary supplements on such important substances. in the present work, a commercial diet used as control diet was enriched with: 1) probiotic shewanella putrefaciens (pdp11 diet, 10(9) cfu g(-1)); 2) probiotic bacillus sp. (bacillus diet, 10(9) ufc g(-1)); 3) aqueous date palm fruits extracts (dpe diet, 4%), and 4) a combination of pdp11 + bacillus sp + aque ...201626712151
differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress.gilthead seabream (sparus aurata l.) is the major cultured fish species in the mediterranean area. high density stocking causes stress and increases the impact of diseases leading to economic losses. probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. the probiotic shewanella putrefaciens, also known as pdp11, was firstly isolated from the skin of healthy gilthead seabream. ...201626617323
isolation and characterization of two lytic bacteriophages, φst2 and φgrn1; phage therapy application for biological control of vibrio alginolyticus in aquaculture live feeds.bacterial infections are a serious problem in aquaculture since they can result in massive mortalities in farmed fish and invertebrates. vibriosis is one of the most common diseases in marine aquaculture hatcheries and its causative agents are bacteria of the genus vibrio mostly entering larval rearing water through live feeds, such as artemia and rotifers. the pathogenic vibrio alginolyticus strain v1, isolated during a vibriosis outbreak in cultured seabream, sparus aurata, was used as host to ...201626950336
application of compound mixture of caprylic acid, iron and mannan oligosaccharide against sparicotyle chrysophrii (monogenea: polyopisthocotylea) in gilthead sea bream, sparus aurata.we have evaluated the therapeutic effect of a compound mixture of caprylic acid (200 mg/kg fish), organic iron (0.2% of diet) and mannan oligosaccharide (0.4% of diet) in gilthead sea bream, sparus aurata linnaeus, infected with sparicotyle chrysophrii beneden et hesse, 1863 in controlled conditions. one hundred and ten reared and s. chrysophrii-free fish (197 g) located in a cement tank were infected by the parasite two weeks following the addition of 150 s. chrysophrii-infected fish (70 g). gr ...201627507773
designation of pathogenic resistant bacteria in the sparusaurata sea collected in tunisia coastlines: correlation with high performance liquid chromatography-tandem mass spectrometry analysis of antibiotics.vibrio is characterized by a large number of species and some of them are human pathogens causing gastro intestinal and wound infections through the ingestion or manipulation of contaminated fishes including vibrio parahaemolyticus and vibrio alginolyticus. in this study, we reported the phenotypic and molecular characterization of vibrio parahaemolyticus and vibrio alginolyticus strains isolated from wild and farm sea bream (sparus aurata l.) along the tunisian coast from december 2015 to april ...201728062288
effects of graded dietary levels of soy protein concentrate supplemented with methionine and phosphate on the immune and antioxidant responses of gilthead sea bream (sparus aurata l.).the effects of a dietary soy protein concentrate (spc) as a fish meal (fm) substitute, on selected innate immune responses, the oxidative status, hepatic and intestinal morphology of gilthead sea bream, sparus aurata, were evaluated after a three-month feeding trial. isonitrogenous (45% crude protein) and isoenergetic (23 kj/g gross energy) diets with 20% (spc20), 40% (spc40) and 60% (spc60) of spc inclusion, supplemented with methionine and phosphate, were evaluated against a diet containing fm ...201728284854
molecular taxonomic profiling of bacterial communities in a gilthead seabream (sparus aurata) hatchery.as wild fish stocks decline worldwide, land-based fish rearing is likely to be of increasing relevance to feeding future human generations. little is known about the structure and role of microbial communities in fish aquaculture, particularly at larval developmental stages where the fish microbiome develops and host animals are most susceptible to disease. we employed next-generation sequencing (ngs) of 16s rrna gene reads amplified from total community dna to reveal the structure of bacterial ...201728261166
the effect of starvation and re-feeding on vasotocinergic and isotocinergic pathways in immature gilthead sea bream (sparus aurata).this study describes the responses of the vasotocinergic and isotocinergic systems to food deprivation and re-feeding processes in immature gilthead sea bream (sparus aurata). the animals were subjected to the following experimental treatments: (1) normal feeding (control), (2) food deprivation for 21 days; and (3) re-feeding for 7 days, beginning 14 days after starvation. the animals were sampled at 0, 7, 14 and 21 days from the beginning of the trial. the pituitary and plasma arginine vasotoci ...201728251326
enhancing gilthead seabream immune status and protection against bacterial challenge by means of antigens derived from vibrio parahaemolyticus.in an attempt to control the proliferation of the pathogenic bacterium vibrio parahaemolyticus in gilthead seabream (sparus aurata), the immunostimulant effect of lysate and toxa from this bacterium was evaluated. fish were intraperitoneally injected twice (first injection, day 1 of the experiment; second injection, day 7) and sampled after one week (on days 8 and 15). afterwards, all fish specimens were experimentally infected with v. parahaemolyticus and mortality was recovered for 1 week. fis ...201727890799
dietary yeast sterigmatomyces halophilus enhances mucosal immunity of gilthead seabream (sparus aurata l.).a yeast was isolated from hypersaline sediments, grown and phylogenetically characterized as sterigmatomyces halophilus strainn16. the dietary administration of this yeast was studied for its effect on skin mucosal immune and antioxidant status of gilthead seabream (sparus aurata l.). fish were fed a commercial diet (control, non-supplemented diet), or the same commercial diet supplemented with 0.55% or 1.1% of yeast for 15 and 30 days. one month after the end of the trial, fish from all treatme ...201728315392
rapid and sensitive detection of lymphocystis disease virus genotype vii by loop-mediated isothermal amplification.lymphocystis disease virus (lcdv) infections have been described in gilthead seabream (sparus aurata l.) and senegalese sole (solea senegalensis, kaup), two of the most important marine fish species in the mediterranean aquaculture. in this study, a rapid, specific, and sensitive detection method for lcdv genotype vii based on loop-mediated isothermal amplification (lamp) was developed. the lamp assay, performed using an apparatus with real-time amplification monitoring, was able to specifically ...201727709436
algerian propolis extracts: chemical composition, bactericidal activity and in vitro effects on gilthead seabream innate immune responses.propolis has been used as a medicinal agent for centuries. the chemical composition of four propolis samples collected from four locations of the sétif region, algeria, using gas chromatography-mass spectrometry was determined. more than 20 compounds and from 30 to 35 compounds were identified in the aqueous and ethanolic extracts, respectively. furthermore, the antimicrobial activity of the propolis extracts against two marine pathogenic bacteria was evaluated. finally, the in vitro effects of ...201728089892
enhanced dietary formulation to mitigate winter thermal stress in gilthead sea bream (sparus aurata): a 2d-dige plasma proteome study.low water temperatures during winter are common in farming of gilthead sea bream in the mediterranean. this causes metabolic disorders that in extreme cases can lead to a syndrome called "winter disease." an improved immunostimulatory nutritional status might mitigate the effects of this thermal metabolic stress. a trial was set up to assess the effects of two different diets on gilthead sea bream physiology and nutritional state through plasma proteome and metabolites. four groups of 25 adult g ...201727882445
molecular details on gilthead sea bream (sparus aurata) sensitivity to low water temperatures from (1)h nmr metabolomics.biometric and metabolic responses of gilthead sea bream to cold challenge are described following a growth trial divided into three water temperature steps, namely cooling, cold maintenance and recovery. experimental data provide a useful description of fish response to thermal stress at both zootechnical and molecular level. although no mortality has been observed, nuclear magnetic resonance-based metabolomics confirms the marked sensitivity of this fish species to low water temperature, and ex ...201727872009
histochemical localization of zinc in the retina cells of gilthead sea bream (sparus aurata) fed different presentations of zinc.to describe the distribution of zinc in the retina of a representative marine fish species and to determine whether the intracellular deposition amount correlates with the presentation of the zinc included in the practical diets (organic, inorganic, encapsulated, fish meal, and control diet), we examined the precise localization of endogenous zinc in the gilthead sea bream (sparus aurata) retina by autometallography. as observed by light microscopy, reaction products were widely distributed thro ...201727862297
histamine and mast cell activator compound 48/80 are safe but inefficient systemic adjuvants for gilthead seabream vaccination.histamine has a key role in the regulation of inflammatory and innate immune responses in vertebrates. gilthead seabream (sparus aurata l.), a marine hermaphrodite teleost of great commercial value, was the first fish species shown to possess histamine-containing mast cells (mcs) at mucosal tissues. mcs are highly abundant in the peritoneal exudate of gilthead seabream and compound 48/80 (co 48/80), often used to promote mc activation and histamine release, is able to promote histamine release f ...201728193449
olive oil bioactive compounds increase body weight, and improve gut health and integrity in gilthead sea bream (sparus aurata).an olive oil bioactive extract (obe) rich in bioactive compounds like polyphenols, triterpenic acids, long-chain fatty alcohols, unsaturated hydrocarbons, tocopherols and sterols was tested (0, 0·08, 0·17, 0·42 and 0·73 % obe) in diets fed to sea bream (sparus aurata) (initial weight: 5·4 (sd 1·2) g) during a 90-d trial (four replicates). fish fed diets containing 0·17 and 0·42 % obe were 5 % heavier (61·1 (sd 1·6) and 60·3 (sd 1·1) g, respectively) than those of the control group (57·0 (sd 0·7) ...201728245885
moderate and sustained exercise modulates muscle proteolytic and myogenic markers in gilthead sea bream (sparus aurata).swimming activity primarily accelerates growth in fish by increasing protein synthesis and energy efficiency. the role of muscle in this process is remarkable and especially important in teleosts, where muscle represents a high percentage of body weight, and by the continuous growth that many fish species present. the aim of this work was to characterize the effects of five weeks of moderate and sustained swimming in gene and protein expression of myogenic regulatory factors, proliferation marke ...201728228414
skin mucus of gilthead sea bream (sparus aurata l.). protein mapping and regulation in chronically stressed fish.the skin mucus of gilthead sea bream was mapped by one-dimensional gel electrophoresis followed by liquid chromatography coupled to high resolution mass spectrometry using a quadrupole time-of-flight mass analyzer. more than 2,000 proteins were identified with a protein score filter of 30. the identified proteins were represented in 418 canonical pathways of the ingenuity pathway software. after filtering by canonical pathway overlapping, the retained proteins were clustered in three groups. the ...201728210224
linking cortisol response with gene expression in fish exposed to gold nanoparticles.fish exposure to environmental stressors (e.g. chemicals, hypoxia, temperature) induce responses enabling them to cope with alterations in their environment. a stress response involves a wide array of changes, from molecular to physiological and behavioural, set to counteract the effect of the stressor and recover homeostatic equilibrium. among other processes, there is activation of the hypothalamus-pituitary-interrenal (hpi) axis, resulting in stimulation of the steroidogenic pathway and relea ...201728169028
untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (sparus aurata).a metabolomic study has been performed to identify sensitive and robust biomarkers of malnutrition in farmed fish, using gilthead sea bream (sparus aurata) as a model. the metabolomic fingerprinting of serum from fasted fish was assessed by means of ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. more than 15,000 different m/z ions were detected and partial least squares-discriminant analysis allowed a clear differentiation between the two exp ...201728168106
extending immunological profiling in the gilthead sea bream, sparus aurata, by enriched cdna library analysis, microarray design and initial studies upon the inflammatory response to pamps.this study describes the development and validation of an enriched oligonucleotide-microarray platform for sparus aurata (saq) to provide a platform for transcriptomic studies in this species. a transcriptome database was constructed by assembly of gilthead sea bream sequences derived from public repositories of mrna together with reads from a large collection of expressed sequence tags (est) from two extensive targeted cdna libraries characterizing mrna transcripts regulated by both bacterial a ...201728165358
bioconcentration and biotransformation of amitriptyline in gilt-head bream.extensive global use of the serotonin-norepinephrine reuptake inhibitor amitriptyline (ami) for treatment of mental health problems has led to its common occurrence in the aquatic environment. to assess ami bioconcentration factors, tissue distribution, and metabolite formation in fish, we exposed gilt-head bream (sparus aurata) to ami in seawater for 7 days at two concentrations (0.2 μg/l and 10 μg/l). day 7 proportional bioconcentration factors (bcfs) ranged from 6 (10 μg/l dose, muscle) to 12 ...201728106990
isolation of lacinutrix venerupis strains associated with disease outbreaks in sea bream sparus aurata and european sea bass dicentrarchus labrax.four gram-negative bacterial isolates were recovered from 2 disease outbreaks that occurred in 2013 affecting european sea bass dicentrarchus labrax fry and sea bream sparus aurata adults. main symptoms were erratic swimming, eroded fins and, in the sea bream outbreak, haemorrhages on the body surface; bacteria were always recovered from internal organs, almost in pure culture. on the basis of phenotypic characterization and 16s rrna gene sequence analysis, the isolates were identified as lacinu ...201728357988
anthropogenic chemical cues can alter the swimming behaviour of juvenile stages of a temperate fish.human pressure on coastal areas is affecting essential ecosystems including fish nursery habitats. among these anthropogenic uses, the seasonal increment in the pressure due to leisure activities such as coastal tourism and yachting is an important environmental stressor in many coastal zones. these pressures may elicit understudied impacts due to, for example, sunscreens or other seasonal pollutants. the island of majorca, northwest mediterranean sea, experiences one of the highest number of to ...201728103523
a novel approach to limit chemical deterioration of gilthead sea bream (sparus aurata) fillets: coating with electrospun nanofibers as characterized by molecular, thermal, and microstructural properties.coating of sea bream fillets with thymol loaded chitosan based electrospun nanofibers (tlcn) and chitosan based nanafibers (cn) has been presented a novel approach to delay chemical deterioration. we assessed cn and tlcn with respect of scanting of total volatile basic nitrogen (tvbn), trimethylamine (tma), thiobarbituric acid (tba) deterioration during cold storage condition. electrospinning process was applied to obtain tlcn and cn. both of nanofibers obtained from biopolymer and bioactive mat ...201728346739
osmoregulatory role of vasotocinergic and isotocinergic systems in the gilthead sea bream (sparus aurata l).gilthead sea bream, sparus aurata l., is an important fish species for the mediterranean aquaculture and is considered a good model for studying the osmoregulatory process, due to its capacity to cope with great changes in environmental salinity (5-60‰). our group studied the osmoregulatory role of different endocrine systems in this species, focusing on the vasotocinergic and isotocinergic systems over several years. for this purpose, the cdnas coding for pro-vasotocin (pro-vt), pro-isotocin (p ...201728065737
tissue-specific gene expression and fasting regulation of sirtuin family in gilthead sea bream (sparus aurata).the seven sirtuin (sirt) counterparts of higher vertebrates were identified and molecularly characterized in a farmed fish of the sparidae family, order perciformes. these proteins are nad(+)-dependent deacetylases that couple protein deacetylation with the energy status of the cell via the cellular nad(+)/nadh ratio with a strict conservation of the characteristic catalytic domain surrounded by divergent n- and c- terminal regions. phylogenetic analysis showed three major clades corresponding t ...201727431591
evaluation of a battery of marine species-based bioassays against raw and treated municipal wastewaters.the present study evaluates a battery of marine species-based bioassays against chemically characterized municipal wastewater samples (raw and wwtp treated). we estimated dunaliella tertiolecta growth rate inhibition (24-96h ic50 values), artemia franciscana immobilization (24h lc50 values), mussel hemocytes viability and lipid peroxidation enhancement (in terms of neutral red retention assay/nrrt and malondialdehyde/mda content, respectively) in influent- and wwtp effluent-treated species. we f ...201727676080
effect of dietary macronutrients on the expression of cholecystokinin, leptin, ghrelin and neuropeptide y in gilthead sea bream (sparus aurata).endocrine factors released from the central nervous system, gastrointestinal tract, adipose tissue and other peripheral organs mediate the regulation of food intake. although many studies have evaluated the effect of fed-to-starved transition on the expression of appetite-related genes, little is known about how the expression of appetite-regulating peptides is regulated by the macronutrient composition of the diet. the aim of the present study was to examine the effect of diet composition and n ...201727725144
quality and antioxidant response of gilthead seabream (sparus aurata l.) to dietary supplements of fenugreek (trigonella foenum graecum) alone or combined with probiotic strains.the present study was conducted to determine the potential effect of the dietary intake of fenugreek (trigonella foenum graecum) seeds alone or in combination with bacillus licheniformis, lactobacillus plantarum or b. subtilis on gilthead seabream quality and antioxidant response after 2 and 3 weeks of experimental feeding. the results showed that the supplements did not affect the percentage of the fatty acid profiles of muscle, demonstrating that all the additives tested can be administrated w ...201728232283
effects of dietary administration of fenugreek seeds, alone or in combination with probiotics, on growth performance parameters, humoral immune response and gene expression of gilthead seabream (sparus aurata l.).the use of immunostimulants is considered a promising preventive practice that may help to maintain animal welfare and a healthy environment, while increasing production and providing higher profits. the purpose of this study was to evaluate the effects on gilthead seabream (sparus aurata l.) of the dietary administration of fenugreek (trigonella foenum graecum) seeds, alone or combined with one of the following probiotic strains: bacillus licheniformis (tsb27), lactobacillus plantarum or bacill ...201727856325
the tongue dorsal surface in fish: a comparison among three farmed species.the oral cavity of vertebrates has a very important role in many functions strictly related to the food processing. two species, sea bass dicentrarchus labrax and seabream sparus aurata, are mainly involved in the aquaculture industry in the mediterranean area; moreover, the white seabream diplodus sargus sargus was recently chosen as the best candidate for the diversification of species of commercial interest. this investigation was carried out, using the standard procedures for light and scann ...201727990675
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