Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| occupational allergy due to spider mites: tetranychus urticae (koch) and panonychus citri (koch). | allergy to both house dust and storage mites is well established, but information about other species of mites is scant. | 1996 | 8955575 |
| mite sensitivity and exposure in the city of santa fe, argentina. | little information is available on mite allergen levels and rates of sensitization to domestic mites in south america. the purpose of this study was to establish the rate of sensitization to domestic mites in asthmatics and measure mite allergen levels in pillows and mattresses of mite allergic individuals in santa fe, argentina. dust samples were collected from the pillows and mattresses of 56 asthmatics (24 females and 32 males, mean age 17.6 +/- 11.5 years), who had been previously skin teste ... | 1996 | 8959537 |
| asthmatic reactions after nasal allergen provocation. | three bronchoconstrictive responses associated with acute rhinitis and occurring in two patients were observed among 400 nasal allergen challenges. causative substances were birch pollen, dermatophagoides pteronyssinus and hsa-conjugated pyromellitic dianhydride. both subjects showed bronchial hyperreactivity and immediate-type sensitization to these allergens. it is concluded that bronchoobstruction nearly occurs in nasal allergen challenges. the underline pathophysiological mechanism remains u ... | 1996 | 8966371 |
| atopy and nonspecific bronchial responsiveness. a population-based assessment. spanish group of the european community respiratory health survey. | the relative importance of specific immunoresponse in explaining nonspecific bronchial responsiveness (br) has scarcely been examined. we provide quantitative estimates of the proportion of br attributable to specific atopy to different common aeroallergens. we used data from a cross-sectional study on a random sample of the general population 20 to 44 yr of age from five spanish areas. there were 1,816 participants who performed a methacholine challenge and had atopy assessed. br was defined as ... | 1996 | 8970347 |
| false-positive skin prick test responses to commercially available dog dander extracts caused by contamination with house dust mite (dermatophagoides pteronyssinus) allergens. | in an outpatient population, a high frequency of positive skin prick test responses to dog dander was found in the absence of detectable ige to dog dander in the rast. the majority of these patients were sensitized to house dust mites (dermatophagoides pteronyssinus) and had no obvious dog-related allergic symptoms. these findings prompted us to investigate whether dog dander skin test preparations are contaminated with house dust mite allergens in amounts sufficient to cause false-positive skin ... | 1996 | 8977501 |
| prevalence of respiratory symptoms, bronchial hyperresponsiveness and atopy among adults: west and east germany. | the prevalence of respiratory symptoms, atopic sensitization and bronchial hyperresponsiveness was compared in a random sample of adults, 20-44 yrs of age, in two cities in west and east germany, hamburg and erfurt, respectively. there were much higher levels of outdoor air pollution due to sulphur dioxide and suspended particulates in erfurt, and major differences in living conditions during the last 40 yrs. within the european respiratory health survey, a short questionnaire was answered by 3, ... | 1996 | 8980967 |
| effect of allergen specific immunotherapy (it) on natural killer cell activity (nk), ige, ifn-gamma levels and clinical response in patients with allergic rhinitis and asthma. | allergen specific immunotherapy (it) has been widely used for many years as a specific treatment of allergic diseases. a variety of changes in immunological parameters have been described but it still remains uncertain as to which of them is responsible for the improvement of symptoms. the aim of our study was to evaluate the effect of it on natural killer (nk) cell activity, il-4, ifn-gamma, ige levels and skin test reactivity in addition to clinical efficacy. thirty-one patients with allergic ... | 1996 | 9015776 |
| fine tuning of epitopic dominance induced by lung cancer on the igg response to bovine betalactoglobulin: towards a paraneoplastic immune marker. | investigating the humoral immune response to mucosal antigens in patients with lung cancer, we have documented a preferential immunoglobulin g (igg) binding to cryptic epitopes unmasked by the proteolysis of bovine beta-lactoglobulin (blg). in contrast, igg from healthy controls and patients with chronic bronchitis (copd) bind preferentially to continuous epitopes presented on both native (n) and denaturated (d) forms of this antigen. the present study further characterized the differences in th ... | 1996 | 8616757 |
| serum total and specific ige levels in children infected with human immunodeficiency virus. | human immunodeficiency virus (hiv) infection is associated with polyclonal b-cell activation, and elevated ige levels are found in infected adults. chronic nasal symptoms, sinusitis, and otitis media are particularly common in children infected with hiv. the possible allergic cause of nasal symptoms in children infected with hiv has not been explored. | 1996 | 8621856 |
| comparative analysis of the genes encoding group 3 allergens from dermatophagoides pteronyssinus and dermatophagoides farinae. | group 3 allergens of the genus dermatophagoides represent one of the major groups of house dust mite allergens. the cdna sequence data for der p 3, in combination with both n-terminal amino acid sequences and substrate affinity data, have confirmed that the group 3 allergens are trypsin-like proteases. using the information from the der p 3 p3ws1 cdna clone, genes encoding both der f3 and der p 3 have now been amplified by the polymerase chain reaction and analysed. two der f3 clones and three d ... | 1996 | 8563488 |
| kinetics and functional implications of th1 and th2 cytokine production following activation of peripheral blood mononuclear cells in primary culture. | the importance of cytokine production in some disease processes is now widely recognized. to investigate temporal relationships between cytokines, we stimulated peripheral blood mononuclear cells (pbmc) in vitro using the t cell mitogen phytohemagglutinin (pha) and various antigens chosen to induce predominantly th1 (streptokinase: streptodornase or purified protein derivative) or th2 (dermatophagoides pteronyssinus, bee or wasp venom: allergens in sensitive subjects) responses. cytokine product ... | 1996 | 8647202 |
| binding affinities of allergens from pollen, mites, and house dust for specific igg subclass antibodies. | the distribution and affinity of igg subclasses against various aeroallergens were assessed by inhibition of specific antibody binding. two parameters from the dose-response curves were taken as indicative of antibody affinity: the point of 50% inhibition and the value of the slopes on double-log plots. it was found that igg4 antibody specific for aeroallergens (i.e., from pollens of several species of gramineae, olea europaea, and parietaria judaica and from house dust) usually exhibits high af ... | 1996 | 8904998 |
| occupational asthma caused by fish inhalation. | occupational asthma (oa) due to fish inhalation, confirmed by specific bronchial challenge (sbc), has not been described as yet in medical literature, as far as we know. we describe two patients whose asthma was induced by occupational exposure to fish and confirmed by serial measurements of pefr and sbc. two fish-processing workers reported asthma symptoms related to their workplace. they were skin tested with fish extracts and their sera assayed for ige antibodies to various fish species. nons ... | 1997 | 9284987 |
| prevalence of allergic sensitization to inhalant allergens among blood donors in kuwait--a desert country. | kuwait is a desert country where the prevailing high temperatures, low humidity, and scant vegetation suggest a low prevalence of allergy. we evaluated the prevalence of atopic sensitization (presence of allergen-specific ige) among young adult blood donors by screening a total of 505 subjects (male: female ratio 1.6) with mean age of 28.4 years (range 18-50 years). the pharmacia cap-phadiatop test, which detects serum ige specific to most common airborne allergens, was used. some of the specifi ... | 1997 | 9450138 |
| allergic sensitisation to common environmental allergens in adult asthmatics in hong kong. | the relationship between immediate skin reactivity to common allergens and adult asthma is not well defined. we retrospectively evaluated the profile of allergen sensitisation to common environmental allergens in 666 adult asthma patients at an asthma clinic in a teaching hospital in hong kong. four hundred and seventy-one asthmatics (70.7%) were atopic; there were more males than females, although the difference did not reach statistical significance. the skin test positively rate decreased sig ... | 1997 | 11850573 |
| the distribution of total and specific serum ige in the european community respiratory health survey. | variations in the prevalence of atopy could provide important clues to the etiology of atopy and asthma. although estimates of prevalence are available from different studies, a lack of standardization makes comparisons difficult. | 1997 | 9058686 |
| allergic and nonallergic interactions between house dust mite allergens and airway mucosa. | asthma and allergy are extremely frequent diseases, affecting 5-10% and 30% of the population, respectively. the prevalence of asthma has increased in many developed countries, which may be due to several factors, including increased exposure to house dust mite (hdm) allergens. hdm to which humans are most frequently sensitized are dermatophagoides pteronyssinus, dermatophagoides farinae, and euroglyphus maynei. these mites multiply in carpets, bedding and upholstered furniture in a hot and humi ... | 1997 | 9073012 |
| allergen skin test reactivities among asthmatic thai children. | skin prick tests with 14 selected local aeroallergens were performed on 100 asthmatic children aged 0-16 years attending the pediatric allergy clinic, faculty of medicine, siriraj hospital. the 14 allergens included dermatophagoides farinae, dermatophagoides pteronyssinus, american cockroach (periplaneta americana), johnson grass (sorghum halopense), cladosporium clarosporoides, alternaria tenuis, penicillium mix, aspergillus mix, cat dander, dog dander, milk, egg white, soy and shrimp. positive ... | 1997 | 9078689 |
| cleavage of the low-affinity receptor for human ige (cd23) by a mite cysteine protease: nature of the cleaved fragment in relation to the structure and function of cd23. | der p i, a cysteine protease representing a major allergen of the house dust mite dermatophagoides pteronyssinus, has recently been shown to cleave cd23 from the surface of cultured human b cells (rpmi 8866 b cell line). we have now undertaken a detailed investigation of cd23 cleavage by der p i. we demonstrate that der p i cleaves cd23 at two sites (ser155-ser156 and glu298-ser299) to produce a 17-kda fragment containing the lectin domain and only part of the c-terminal tail. no such effect was ... | 1997 | 9079796 |
| stimulation of blood mononuclear cells of atopic children with the relevant allergen induces the release of eosinophil chemotaxins such as il-3, il-5, and gm-csf. | peripheral blood mononuclear cells (pbmc) from 10 atopic asthmatic children (atopics), sensitized to dermatophagoides pteronyssinus (dp), and from 5 nonatopic healthy children (controls) were stimulated with dp extract or with birch extract (be). after 6 days we tested the supernatant's (sn) chemotactic activity toward purified blood eosinbnophils and t-lymphocyte proliferation. dp induced a statistically significant t-cell proliferation from atopics as compared to controls (p < 0.05), which cor ... | 1997 | 9088301 |
| allergen content in dust from homes and schools in northern norway in relation to sensitization and allergy symptoms in schoolchildren. | previous studies have shown a high prevalence of atopic diseases among school children in the community of sør-varanger. moreover, animal dander followed by pollen and house dust mite, were the most common allergens in skin prick tests. | 1997 | 9088651 |
| asthma and its relation with dermatophagoides pteronyssinus and dermatophagoides farinae in andean altitudes (quito, ecuador). | previous investigations in alpine altitudes suggest a very low etiopathogenic relation between house-dust mites and bronchial asthma in these geographical zones. our purpose was to analyze the relationship between asthma and sensitization to the house-dust mites d. pteronyssinus and d. farinae in andean altitudes. we studied 587 asthmatic patients, residents in quito, ecuador, (2800 m above sea level), ranging in age from 8 months to 84 years. the patients were divided into five age groups. we i ... | 1997 | 9093934 |
| house dust mite allergen levels in public places in new zealand. | house dust mite allergens are a risk factor for asthma in new zealand, and levels in domestic dwellings have been found to be high compared with levels in most other countries. studies in other countries have demonstrated lower levels of dermatophagoides pteronyssinus allergens in public places compared with levels in domestic dwellings. | 1997 | 9155822 |
| transfer of atopy following bone marrow transplantation. | bone marrow transplantation is an optimal treatment of acute leukemia and aplastic anemia. allergic manifestations in recipients long after bone marrow transplantation have been reported. a case involving transfer of atopy manifested as food allergy-induced-atopic dermatitis is reported. | 1997 | 9164366 |
| rapid expression of the cd69 antigen on t cells and natural killer cells upon antigenic stimulation of peripheral blood mononuclear cell suspensions. | the cd69 antigen has been identified as the earliest activation marker on the surface of cytokine- or mitogen-activated lymphocytes. the expression of this molecule may be a useful early marker of antigen- or allergen-specific activation of lymphocytes in vitro. we evaluated the expression of the cd69 and cd25 antigens on antigen- or allergen-stimulated lymphocytes and the proliferative responses as detected by thymidine incorporation. peripheral blood mononuclear cells (pbmc) of allergic patien ... | 1997 | 9188933 |
| il-13 production by allergen-stimulated t cells is increased in allergic disease and associated with il-5 but not ifn-gamma expression. | interleukin-13 (il-13) shares many, but not all, of the properties of the prototypic t-helper type 2 (th2) cytokine il-4, but its role in allergen-driven t-cell responses remains poorly defined. we hypothesized that allergen stimulation of peripheral blood t cells from patients with atopic disease compared with non-atopic controls results in elevated il-13 synthesis in the context of a 'th2-type' pattern. freshly isolated peripheral blood mononuclear cells (pbmc) obtained from sensitized atopic ... | 1997 | 9203965 |
| the most common phenotypes of sensitization to inhalant allergens in childhood. | atopic individuals are frequently sensitized to a limited number of certain allergens, although most of them are exposed to multiple inhalant allergens in daily life. | 1997 | 9208185 |
| functional cd86 (b7-2/b70) is predominantly expressed on langerhans cells in atopic dermatitis. | recently, we reported the functional expression of cd86 on cultured human langerhans cells derived from normal epidermis. in the present study, we investigated the expression and function of co-stimulatory molecules in the pathogenesis of atopic dermatitis. in immunohistochemical analysis, cd80 and/or cd86 were detected on dendritic-shaped cells not only in the epidermis but also in the dermis in the inflammatory lesions of atopic dermatitis (n = 12). cd80 was expressed in only five cases (42%), ... | 1997 | 9217814 |
| determination of the n- and c-terminal sequences required to bind human ige of the major house dust mite allergen der f 2 and epitope mapping for monoclonal antibodies. | b cell epitopes of the major house dust mite allergen der f 2 from dermatophagoides farinae were analysed using deletion mutants of der f 2 expressed as fusion proteins in escherichia coli. the reactivities of these partial der f 2 molecules to human anti-mite ige antibodies in atopic patients and to murine anti-der f2 monoclonal antibodies (mabs) were examined by immunoblotting. a c-terminal deletion mutant of der f 2, 1-123, had almost the same reactivity to human ige as the whole der f 2 (1-1 ... | 1997 | 9224967 |
| prevalence of wheeze and asthma and relation to atopy in urban and rural ethiopia. | asthma and allergy in developing countries may be associated with adoption of an urbanised "western" lifestyle. we compared the rates of asthma symptoms and atopy in urban populations in jimma, southwest ethiopia, at an early stage of economic development with those among the population of remote, rural, subsistence areas, and assessed the potential role of environmental aetiological factors leading to the differences. | 1997 | 9228959 |
| house dust mite and cat allergen levels in domestic dwellings in christchurch. | to quantify the levels of dermatophagoides pteronyssinus allergen (der p i) and felis domesticus allergen (fel d i) in domestic dwellings in christchurch and to assess possible relationships with housing characteristics. | 1997 | 9236806 |
| prevalence of childhood asthma based on questionnaires and methacholine bronchial provocation test in korea. | in most epidemiological survey studies, only subjective symptoms and past medical history of asthma have been used as diagnostic criteria. even though a questionnaire survey can be performed in a large population study at low cost, limitations such as lack of objectivity and poor predictability in non-specific bronchial hyperresponsiveness cannot be avoided. | 1997 | 9249268 |
| adhesion molecules in atopic dermatitis: upregulation of alpha6 integrin expression in spontaneous lesional skin as well as in atopen, antigen and irritative induced patch test reactions. | atopic dermatitis is a lymphocyte-mediated skin disease. we studied the expression of the adhesion molecule alpha6 integrin by immunohistochemistry in spontaneous atopic inflammation as well as during the eliciting phase of atopen (dermatophagoides pteronyssinus), antigen (nickel sulfate) and irritative (anthralin) induced patch test reactions in atopic skin. results were compared with nickel sulfate patch test reactions in normal skin. a role of the alpha6 integrin, expressed at the luminal sid ... | 1997 | 9250597 |
| exposure to indoor allergens in early infancy and sensitization. | indoor allergens play a major role both in sensitization and as triggers of asthma in children. the relationship between allergen exposure and sensitization to cats, dogs, and mites was studied prospectively in 100 newborn babies with a history of allergy in both parents. | 1997 | 9275137 |
| identification of the domestic mite fauna of puerto rico. | this study was conducted to identify the domestic mite fauna of puerto rico. a total of 57 dust samples were collected from mattresses in homes of 11 cities on the island. the analysis of the samples revealed that 73.70% of the mattress samples had at least one mite species. the identified species include: dermatophagoides pteronyssinus (45.6%), blomia tropicalis (31.6%), cheyletus sp. (19.3%), dermatophagoides farinae (17.5%), euroglyphus maynei (5.3%), dermatophagoides sibonei (1.8%), dermatop ... | 1997 | 9285987 |
| mould-devouring mites differ in guanine excretion from dust-eating acari, a possible error source in mite allergen exposure studies. | measurement of guanine in dust proved a good assessment of mite allergen exposure. | 1997 | 9291290 |
| immune memory in cd4+ cd45ra+ t cells. | this study addresses the question of whether human peripheral cd4+ cd45ra+ t cells possess antigen-specific immune memory. cd4+ cd45ra+ t cells were isolated by a combination of positive and negative selection. putative cd4+ cd45ra+ cells expressed cd45ra (98.9%) and contained < 0.1% cd4+ cd45ro+ and < 0.5% cd4+ cd45ra+ cd45ro+ cells. putative cd45ro+ cells expressed cd45ro (90%) and contained 9% cd45ra+ cd45ro+ and < 0.1% cd4+ cd45ra+ cells. the responder frequency of dermatophagoides pteronyss ... | 1997 | 9301520 |
| biological activity of recombinant der p 2, der p 5 and der p 7 allergens of the house-dust mite dermatophagoides pteronyssinus. | der p 2, der p 5 and der p 7 are three allergens of the house-dust mite dermatophagoides pteronyssinus that have been cloned and expressed in escherichia coli as fusion proteins with glutathione-s-transferase (gst). we showed that these recombinant allergens produced immediate hypersensitivity skin-test reactions in 70, 60 and 52% respectively of a group of mite-sensitive allergic patients who were strongly positive to whole mite extract (wme). comparable positivities were found for serum levels ... | 1997 | 9303332 |
| potentiation of histamine release against inhalant allergens (dermatophagoides pteronyssinus) with bacterial antigens in bronchial asthma. | in the etiopathogenesis of bronchial asthma, the important role of bacterial infection is more evident every day, favoring inflammation and obstruction, that is, triggering an asthmatic response. we gathered 36 patients diagnosed of bronchial asthma with sensitization to dermatophagoides pteronyssinus and 32 healthy subjects. histamine release tests against staphylococcus aureus extract alone or together with d. pteronyssinus were performed, and the results were contrasted with or without the pr ... | 1997 | 9330183 |
| ige antibodies to aeroallergens in allergic children in são paulo, brazil. | the sensitization to inhaled allergens was studied in 80 brazilian children and 12 controls, aged 6 to 16 years. we performed skin tests with alternaria alternata, cat, dog, lolium perenne, grasses and the following domestic mites: blomia tropicalis, dermatophagoides pteronyssinus, dermatophagoides farinae, lepidoglyphus destructor, chortoglyphus arcutus and aleuroglyphus ovatus. the frequencies of positive skin tests (mean diameter > or = 23 mm) were respectively 15%, 11%, 11%, 6%, 7%, 95%, 92% ... | 1997 | 9330189 |
| lack of lysozyme activity of natural and yeast-derived recombinant der p 2. | for dermatophagoides pteronyssinus a number of allergens have been reported. although the function of several allergens is known, that of the house dust mite allergen der p 2 is unknown. a role as lysozyme has been suggested. we tested a yeast-derived recombinant der p 2 for its lysozymatic activity. this recombinant allergen and affinity-purified natural der p 2 lacked this enzymatic activity. whole-body mite extract retained its lysozymatic activity after removal of the group 2 allergen by mon ... | 1997 | 9338615 |
| salmeterol inhibits the allergen-induced mononuclear cell proliferation and downregulates gm-csf release and hla-dr expression by monocytes. | beta 2-adrenoceptor agonists are known to attenuate several functions of human mononuclear cells in response to activation. since monocytes and lymphocytes play a major pathogenetic role in allergic asthma, this study was designed to evaluate the hypothesis that salmeterol, a beta 2-adrenoceptor agonist with a long duration of action, could inhibit in vitro the allergen-induced activation of blood mononuclear cells (bmcs). bmcs were collected from subjects sensitized to dermatophagoides pteronys ... | 1997 | 9344832 |
| occupational allergic disease in cereal workers by stored grain pests. | it is well known that workers occupationally exposed to grain dust have a high prevalence of respiratory symptoms, but their pathogenesis remains obscure when sensitization to cereal flour cannot be demonstrated. storage mites, tenebroids, and cockroaches are stored-grain pests found in grain and cereal products frequently in our area, where the cereal industry is the most important industry. an epidemiological analysis of sensitization of these stored-grain pests was performed on 4379 patients ... | 1997 | 9350153 |
| gm-csf increases the ability of cultured macrophages to support autologous cd4+ t-cell proliferation in response to dermatophagoides pteronyssinus and ppd antigen. | previous studies have demonstrated an infiltration of monocytes and increased levels of granulocyte-macrophage colony-stimulating factor (gm-csf) in the asthmatic lung. to study the possible effects of this cytokine upon the differentiation and function of these newly recruited monocytes, we have developed a model in which monocytes isolated from human peripheral blood were differentiated into macrophages in serum in the presence or absence of gm-csf. after 7 days, the macrophages increased in s ... | 1997 | 9370934 |
| comparison of mrast and cap with skin end point titration for alternaria tenuis and dermatophagoides pteronyssinus. | there has been a recent explosion of new in vitro tests for the diagnosis of allergies. at present there is no general agreement on which type of in vitro test is best. recently our hospital switched in vitro testing from the modified radioallergosorbent system (mrast) to the pharmacia cap system (cap). while changing in vitro testing techniques, 47 patients were tested with both the mrast and cap tests. comparisons were made between the mrast and cap results of alternaria tenuis and dermatophag ... | 1997 | 9374169 |
| analysis of total ige and allergen-specific ige antibody levels of allergic children in taiwan. | with advances in technology, several in vitro screening tests such as mast and cap system have been used for analyzing the allergens involved in allergic diseases such as bronchial asthma (ba), allergic rhinitis (ar), atopic dermatitis (ad) and urticaria. in this study, cap system (pharmacia, sweden) was used to screen the prevalence of allergens responsible for these atopic diseases. a total of 392 children were enrolled in this study retrospectively, all these atopic children visited the aller ... | 1997 | 9401182 |
| ige responses to dermatophagoides pteronyssinus native major allergens der p 1 and der p 2 during long-term specific immunotherapy. | we investigated by elisa the ige response to whole extract of the house-dust mite dermatophagoides pteronyssinus (dp) and to the native major allergens, der p 1 and der p 2, in sera from 18 adult patients (group a) with dp-allergic asthma before (t0) and 1, 2, 3, and 4 (t1-t4) years after subcutaneous specific immunotherapy (sit). a qualitative reduction (p = 0.05) of the ige responses to dp and der p 2 was observed from t1 to t4, but a highly statistical significant decrease appeared at t3 (p < ... | 1997 | 9404565 |
| the effect of frequent vacuum cleaning on the house dust mite allergen, der p 1 in carpets: a pilot study. | to determine the effect of frequent vacuum cleaning of carpets on der p 1, the major group one allergen of the house dust mite dermatophagoides pteronyssinus. | 1997 | 9418840 |
| use of thiocyanate elution to estimate relative avidity of allergen specific ige antibodies. | although several methods for estimating avidity of antigen-antibody reactions are available, most are impractical for the study of human ige antibodies because of a requirement for pure allergen and antibody in relatively large amounts. to determine the relative avidity of specific ige antibodies for dermatophagoides pteronyssinus allergens, seven concentrations of the chaotropic thiocyanate ion were used to disrupt epitope-antibody binding in a specific ige immunoassay system, using sera from 1 ... | 1997 | 9429669 |
| evaluation of the mast cla allergy system for diagnosis of allergies to house dust mites and cats. | the mast cla system was evaluated against skin prick test (spt) for diagnosis of allergies to house dust mites (dermatophagoides pteronyssinus, dermatophagoides farinae) and cats. forty three asthmatic children were examined by spt and mast cla. chi-square analysis indicated significant association between spt and mast cla results for the house dust mites but not for cats. the sensitivities of mast cla for house dust mites and cats were 100 and 25% respectively; specificities were all less than ... | 1997 | 9438543 |
| kinetics of allergen expression in cultures of house dust mites, dermatophagoides pteronyssinus and d. farinae (acari: pyroglyphidae). | laboratory cultures of house dust mites dermatophagoides pteronyssinus (trouessart, 1897) and dermatophagoides farinae hughes, 1961 were used to study the population dynamics of the mites and the kinetics of antigen appearance. the analysis of extracts obtained after different incubation periods, carried out by sds-page, immunoblotting, and enzyme-linked immunosorbent assay, allows for the definition of 3 different growth phases: the latency phase (f1); the exponential growth phase (f2) during w ... | 1997 | 9439123 |
| comparison of major aeroallergens in taipei and kin-men. | ragweed pollen is an important aeroallergen in the usa, however, its role in the pathogenesis of allergic airway diseases in taiwan has not been reported. recently, a dramatic increase in patients with allergic rhinitis was noted in kin-men. most patients had characteristic seasonal variation with the symptoms waxing in august and waning in november, which correlated with the pollen season of ragweed. the aim of this study was to clarify the role of ragweed pollen in the pathogenesis of allergic ... | 1997 | 9444919 |
| allergen challenge of passively sensitized human bronchi alters m2 and beta2 receptor function. | we investigated whether antigen challenge may alter m2 and beta2 receptor function in isolated passively sensitized human bronchi. bronchial rings (2-4 mm internal diameter) were obtained from 12 patients. passive sensitization was induced by serum containing high ige levels to dermatophagoides pteronyssinus and dermatophagoides farinae. rings from six patients were used to study m2 receptor function by incubating with cumulatively increasing concentrations (10(-8) m to 10(-4) m) of pilocarpine ... | 1997 | 9105059 |
| rush immunotherapy results in allergen-specific alterations in lymphocyte function and interferon-gamma production in cd4+ t cells. | allergen immunotherapy results in a number of changes in clinical, inflammatory, and immunologic parameters. however, the basis for the specificity of this form of therapy is unknown, especially in the context of changes in t- and b-lymphocyte function after desensitization to specific allergens. | 1997 | 9111499 |
| il-5 secretion by allergen-stimulated cd4+ t cells in primary culture: relationship to expression of allergic disease. | il-5-producing allergen-specific t cells are thought to play a prominent role in the pathogenesis of allergic inflammation. we hypothesized that t cell allergen-driven il-5 synthesis is elevated in patients with atopic disease as compared with that in atopic patients free of disease and nonatopic control subjects. | 1997 | 9111503 |
| prenatal allergen contact with milk proteins. | cellular proliferation to various allergens (dermatophagoides pteronyssinus, beta-lactoglobulin, bovine serum albumin, ovalbumin) has been found in cord blood cells. whether this reflects a sensitization during foetal life is uncertain. | 1997 | 9117877 |
| feather mites are potentially an important source of allergens for pigeon and budgerigar keepers. | previous studies on allergy to feathers have not addressed whether organisms living on feathers (mites, lice, moulds) are a source of allergens. | 1997 | 9117882 |
| evaluating the factors that relate to asthma severity in adolescents. | over the past 5 years, we have been engaged in a cross-sectional evaluation of risk factors for higher asthma severity in adolescents aged 13-18. all recruitment takes place through public and private schools. the sample from which our current findings are derived includes 151 adolescents covering a wide spectrum of asthma severity and socioeconomic status (ses) and representing both african american and caucasians. an asthma severity instrument has been developed and validated for the purpose o ... | 1997 | 9130491 |
| prevalence of skin reactions to aeroallergens in asthmatics of puerto rico. | in puerto rico, although a high prevalence of asthma has been reported, the sensitization rates to aeroallergens in these patients is unknown. the purpose of this study using a case control design, was to determine and compare the rates of sensitization to common aeroallergens in an asthmatic population of 576 asthmatics and 144 healthy controls. a skin prick test was conducted using standardized extracts of dermatophagoides farinae (df) and d. pteronyssinus (dp), house dust (hd), cat hair and e ... | 1997 | 9580406 |
| indoor allergens and dwelling characteristics in two cities in spain. | indoor allergens are common causes of sensitization and asthma. climatic and home conditions can modify their levels. | 1997 | 9491197 |
| [the incidence of peculiar ige in response to inhalant allergens in relation to the age of the patient]. | 208 patients between the age of 1 month to 56 years, with symptoms of respiratory tract allergy were investigated. in all cases serum titre of sige to birch, thymoty, rye, mugwort pollen, alternaria alternata, dog, cat fur, dermatophagoides pteronyssinus and farinae was determined with the use of quidel immunoenzymatic method. we have found sige to alternaria alternata in 8 (38%), birch pollen in 5 (23.8%) dog fur in 5 (23.8%) and thymoty and rye pollen in 3 (14.3%) even among one years-old infa ... | 1997 | 9518328 |
| purification and characterization of a 94 kd high molecular weight allergen from house dust mite, dermatophagoides pteronyssinus. | house dust mite allergens from dermatophagoides pteronyssinus is an important cause of severe allergic asthma and rhinitis in many countries. although several low to medium molecular weight allergens had been well characterized, limited studies on the high molecular weight ige-binding components were reported. in this study, a 94 kd high molecular weight allergen from crude mite body extract of d. pteronyssinus was purified and characterized. monoclonal antibody (mab) affinity chromatography and ... | 1997 | 10592827 |
| a dermatitis flare after patch testing with dermatophagoides pteronyssinus in an atopic patient. | 1997 | 9255502 | |
| a reason why measures to avoid mite-induced asthma are more effective in children than in adults. | mite-sensitive asthmatics seem to respond more markedly to a reduction in mite allergen in the bedding if they are children than if they are adults. in order to investigate this difference, 882 children who attended our allergy clinic were studied. the accompanying parents were asked standardized questions, and skin prick tests to dermatophagoides pteronyssinus and d. farinae were performed on the children. the number of hours that the children spent in bed lessened progressively with increasing ... | 1997 | 9617778 |
| a prospective safety-monitoring study of immunotherapy in mite-allergy patients with mass-units-standardized extract. | to determine the tolerance of an allergen extract standardized in mass units, we closely monitored the side-effects, during the buildup phase of immunotherapy treatment, in 88 patients with well-documented respiratory allergy to house-dust mite (dermatophagoides pteronyssinus). thirty-four patients (38.6%) suffered from moderate perennial rhinitis, and 54 had mild to moderate bronchial asthma (61.4%). for the desensitizing treatment, we used a depot extract adsorbed in aluminum hydroxide of d. p ... | 1997 | 9201372 |
| allergen binding to specific ige in the nasal mucosa of allergic patients. | until now, it has not been possible to identify specific ige locally in the airway mucosa. in this study we investigated the possibility of determining specific allergen binding locally in the nasal mucosa. | 1997 | 9111497 |
| immunotherapy with a standardized dermatophagoides pteronyssinus extract. vi. specific immunotherapy prevents the onset of new sensitizations in children. | the natural history of allergic sensitization is complex and poorly understood. a prospective nonrandomized study was carried out in a population of asthmatic children younger than 6 years of age whose only allergic sensitivity was to house dust mites (hdms). | 1997 | 9111487 |
| house dust mite allergens in saudi arabia: regional variations and immune response. | in order to assess the causative extrinsic allergic factor(s) in school-age children diagnosed as having bronchial asthma and allergic rhinitis, and to qualitatively and quantitatively evaluate the presence of house dust mites (hdms) in the homes of these children in saudi arabia, a study analyzing mite contents in 165 samples collected from patientsâ indoor environment was conducted. the dust samples were collected from four regions of saudi arabia, showing variation in their geography and clim ... | 1997 | 17377421 |
| a preceding airway reaction to one allergen may lead to priming of the airway responses to another allergen. | this study aimed to determine whether a preceding airway response to one allergen leads to priming of the airway responses to another allergen. twelve asthmatic children who had positive prick tests to two allergens, dermatophagoides pteronyssinus (d.p.) and german cockroach (cr), participated in a randomized, placebo-controlled crossover study. we performed two consecutive inhalation challenges, d.p. challenge being followed 48 h later by cr challenge. the effect of initial (d.p.) challenge on ... | 1997 | 9140518 |
| specific immunotherapy with dermatophagoides pteronyssinus and d. farinae results in decreased bronchial hyperreactivity. | to evaluate the tolerability and efficacy of specific immunotherapy with mite extracts, we performed a double-blind, placebo-controlled immunotherapy study in 30 patients with proven allergy to mite allergens. the specific immunotherapy with standardized extracts of dermatophagoides pteronyssinus and d. farinae by a clustered rush protocol was well tolerated. after 1 year of treatment, the actively treated group showed a significant improvement compared to their starting value as well as to the ... | 1997 | 9140517 |
| spetial and temporal distribution of house dust mite (astigmata:pyroglyphidae) allergens der p 1 and der f 1 in barbadian homes. | house features contribute to house dust mite abundance and, therefore, exposure to mite allergens. our study assessed the hypothesis that modernization of the domestic environment in a tropical setting may lead to a level of allergen from the house dust mites dermatophagoides pteronyssinus (trouessart) and d. farinae hughes that previously has been defined clinically as at risk for people who suffer from allergic disease. allergen (der p 1 and der f 1) levels were measured at 4 sites (mattress, ... | 1997 | 9103765 |
| mite allergy is associated with a specific profile of igg epitopes recognized on antigen p1 of dermatophagoides pteronyssinus. | immune response to inhaled antigens differs in allergic patients and healthy individuals, mostly in the quality of t cell help provided (i.e. th2 or th1 dominant subset). however, while different in many functional aspects, both groups of t cells shared the capacity to support the synthesis of antigen-specific immunoglobulin g (igg) antibodies detected in different amounts in the serum of atopic and healthy individuals. | 1997 | 9088656 |
| immunotherapy with standardized extract of dermatophagoides pteronyssinus in bronchial asthma: a dose-titration study. | according to the maximum tolerated dose (mtd) achieved, we assessed the changes in clinical and laboratory parameters, induced by specific immunotherapy (sit), in a group of 43 asthmatic patients sensitized to dermatophagoides pteronyssinus, over a period of 18 months. a standardized extract (100 bu/ml; 40 micrograms/ml of der p 1; 20 micrograms/ml of der p 2) was used. the patients were divided into two groups: the high-dose immunotherapy (hdi) group (mtd > or = 4 micrograms der p i) and the co ... | 1997 | 9105521 |
| heterogeneous proteolytic specificity and activity of the house dust mite proteinase allergen der p i. | exposure of the skin or respiratory tract to proteinases is frequently associated with allergic sensitization. this is of particular significance in the domestic indoor environment where the proteolytic activity of der p i, the group i allergen of the house dust mite dermatophagoides pteronyssinus, may influence the allergenicity of mites. using class-specific proteinase inhibitors and active-site affinity chromatography, we have previously shown that der p i exhibits a mixed cysteine-serine pro ... | 1997 | 9061221 |
| effect of a bed covering system in children with asthma and house dust mite hypersensitivity. | allergen avoidance is regarded as an important approach to management of atopic asthma. the effect of intervent bed covering systems on house dust mite (hdm) allergen exposure, asthma symptoms and markers of inflammation was investigated in 31 hdm sensitive asthmatic children. dust concentrations of dermatophagoides pteronyssinus allergen 1 (der p 1) were monitored before and after covering the mattress, duvet and pillow with active and placebo covers for 3 months, in a single-blind, cross-over ... | 1997 | 9042633 |
| prediction of murine mhc class i epitopes in a major house dust mite allergen and induction of t1-type cd8+ t cell responses. | the group i (der p 1) allergen of dermatophagoides pteronyssinus (house dust mite, hdm) contains several t helper (th) epitopes recognized by c57bl/6 mice, with the peptide (111-139) containing a dominant mhc class ii-restricted epitope (113-127). since cd8+ t cells are thought to play a role in the regulation of allergic disease, we examined the der p 1 sequence for potential mhc class i-binding motifs and observed that residues 111-119 (fgisnycqi) contain motifs for h-2db and kb. furthermore, ... | 1997 | 9040009 |
| germline tcr-a restriction of immunoglobulin e responses to allergen. | immunoglobulin e responses to known environmental antigens (allergens) may serve as a general model to investigate germline genetic restriction of the immune response. we have previously shown genetic linkage between ige responses to major allergens and the t-cell receptor (tcr) a/d locus, but not to tcr-b, implying that elements in tcr a/d restrict the ability to react to specific antigens. we now show, in two sets of subjects from the same population, a strong allelic association between a va8 ... | 1997 | 9211749 |
| house dust mite immunotherapy results in a decrease in der p 2-specific ifn-gamma and il-4 expression by circulating t lymphocytes. | allergen-specific immunotherapy (it) can be an important adjunctive therapy in the treatment of allergic disorders. although it has now been used for over 80 yr, the precise mechanism of action remains unclear. recently a number of studies have shown that cytokine production may be modified by it, but different protocols have been used and different results obtained. | 1997 | 9117880 |
| skin prick reaction and nasal provocation response in diagnosis of nasal allergy to the house dust mite. | the allergen skin test is commonly used to ensure the diagnosis of allergic rhinitis even though positive results do not necessarily indicate that rhinitis is of allergic origin. | 1997 | 9396976 |
| recruitment of circulating allergen-specific t lymphocytes to the lung on allergen challenge in asthma. | in allergic subjects with asthma, the migration of cd4+ t cells to the lungs in the hours after allergen exposure may contribute to allergic inflammation in the target organ. | 1997 | 9389298 |
| predictors of early- and late-phase reactions to bronchial allergen challenge. | the influence of inhaled steroids and predictive factors on the response to bronchial allergen challenge (bca) was evaluated in 80 asthmatics allergic to dermatophagoides pteronyssinus (der p). all underwent bca with der p and measurement of early (ear) and late asthmatic reaction (lar). the cumulative dose of allergen producing 20% fall in fev1 in the ear (pd20) was calculated. bronchial histamine provocation, conjunctival provocation test (cpt), and skin prick test with der p extract were perf ... | 1997 | 9360751 |
| phospholipid metabolism in european house dust mite (acari: pyroglyphidae). | phospholipid metabolism was studied in european house dust mite, dermatophagoides pteronyssinus (trouessart). analysis of phospholipid metabolites in mite extracts suggested the presence of plasea1, plasea2, and plasec, which have important physiological roles in phospholipid metabolism. the optimum ph of plasea1 and plasea2 was 8 and 9, and the optimum temperature of plasea1 was 45 degrees c; the activity of plasea2 was not affected by temperature. the activity of plasea1 at ph 8 and 37 degrees ... | 1997 | 9379459 |
| allergen-induced cytokine production in atopic disease and its relationship to disease severity. | the th2 cytokines, interleukin (il)-4 and il-5, have an important role in atopic disease. cd30 is a transmembrane molecule that may be expressed on a proportion of activated t-lymphocytes and has been reported to be a marker for th2 phenotype. our objective was to compare the in vitro cytokine responses and cd30 expression of peripheral blood mononuclear cells (pbmcs) to stimulation with house dust mite antigen (dermatophagoides pteronyssinus) in atopic asthmatics, atopic nonasthmatics, and norm ... | 1997 | 9308924 |
| hla antigens in asthmatic children. | the purpose of the present study was to analyze the frequency of hla-dr and dq antigens in brazilian asthmatic children with skin-test and rast positivity to dermatophagoides pteronyssinus. the comparison of hla-dr and dq antigenic frequencies between patients (n = 30) and controls disclosed a significantly higher hla-dq2 frequency in the patients (60% versus 34%, p = 0.013; r. r. = 2.8). | 1997 | 9532256 |
| skin test and crossreactivity studies with euroglyphus maynei and dermatophagoides pteronyssinus. | the prevalence of sensitization to euroglyphus maynei (e. maynei) in the united states has not been reported previously. | 1997 | 9291285 |
| t-cell sensitization to epitopes from the house dust mites dermatophagoides pteronyssinus and euroglyphus maynei. | dermatophagoides pteronyssinus and euroglyphus maynei frequently occur in house dust but little is known about primary sensitization to the less abundant e. maynei. | 1997 | 9291282 |
| a 1-year, placebo-controlled, double-blind house-dust-mite immunotherapy study in asthmatic adults. | thirty-one adult patients with asthma caused by house-dust mites (hdm) were included in this placebo-controlled, double-blind study to evaluate the efficacy and safety of specific immunotherapy (sit) with biologically standardized extracts of hdm. the specific diagnosis was confirmed by skin prick tests, specific ige, and bronchial provocation tests with hdm allergens. the patients were randomized to receive active treatment with extracts of either dermatophagoides pteronyssinus (dpt) or d. fari ... | 1997 | 9284985 |
| exposure chamber for allergen challenge. a placebo-controlled, double-blind trial in house-dust-mite asthma. | exposure chambers have proven to be valuable tools in the study of reactions to aeroallergens, and in monitoring the efficacy of antiallergic therapy. in the present study, 15 house-dust-mite-allergic asthmatics and five nonallergic volunteers were challenged in a recently developed exposure chamber. the trial was performed double-blinded with house-dust-mite allergen or placebo. patients with allergy to house-dust mite (dermatophagoides pteronyssinus) (der p) were included by positive skin pric ... | 1997 | 9284981 |
| differential mrna expression and production of interleukin-4 and interferon-gamma in stimulated peripheral blood mononuclear cells of house-dust mite-allergic patients. | optimal culture conditions were established for the analysis of interleukin-4 (il-4) and interferon-gamma (ifn-gamma) mrna expression and protein production, as well as proliferative capacity of peripheral blood mononuclear cells (pbmc). these culture conditions permitted the analysis of differences in the responses of house-dust mite (hdm) allergic patients and healthy controls after polyclonal and allergen-specific stimulation. proliferative responses were optimal when pbmc were cultured in rp ... | 1998 | 9613681 |
| [nedocromil sodium is effective in the treatment of mild and moderate allergic asthma caused by dermatophagoides pteronyssinus]. | we have evaluated the efficacy of nedocromil sodium in 38 patients (18 males and 20 females, mean age 37.2 yrs) suffering from mild or moderate allergic asthma due to dermatophagoides pteronissinus. nedocromil sodium was given twice a day at the dosage of 4 mg each time for two months. on a daily diary card patients had to record the severity and the number of asthmatic attacks, the usage of bronchodilators and the values of peak respiratory flow measured in 1/min in the morning and at night. th ... | 1998 | 9586423 |
| clinical effects of benzyl benzoate in the prevention of house-dust-mite allergy. results of a prospective, double-blind, multicenter study. | the efficacy of the acaricide benzyl benzoate as an additive to a chemically and technically defined cleaning substance (acarosan) was tested in a multicentric, prospective, randomized, controlled study on 118 outpatients with bronchial asthma due to house-dust-mite allergy. subjective reports from patients and doctors revealed an improvement in clinical complaints in more than 50%, with only small differences between the verum and the placebo group. objective parameters such as titrated skin te ... | 1998 | 9574889 |
| reproducibility of skin prick test results in epidemiologic studies: a comparison of two devices. | the reproducibility of skin prick tests under field conditions is essential for comparing prevalences between centers in epidemiologic multicenter studies. this study aimed to evaluate and compare the reproducibility of two widely used skin prick test devices: the multi-test and the alk lancet. the subjects were 28 children, aged 6-14 years, with known sensitivities to dermatophagoides pteronyssinus (d. pter.). both devices were applied to each subject on two occasions, 1 week apart, by differen ... | 1998 | 9574876 |
| changes in serum haptoglobin level after allergen challenge test in asthmatic children. | bronchial asthma is characterized by airway inflammation, which underlies the phenomenon of bronchial hyperresponsiveness. previous studies have shown that this correlates with the serum concentration of haptoglobin. the occurrence of the late asthmatic response (lar) after an allergen challenge test is associated with airway inflammation. the objectives of this study were to examine serum levels of haptoglobin during the 24 h after allergen challenge and to compare changes between the subjects ... | 1998 | 9534918 |
| cross-reactivity between terrestrial snails (helix species) and house-dust mite (dermatophagoides pteronyssinus). ii. in vitro study. | epidemiologic and in vitro data have shown that the association of house-dust mite (hdm) allergy and snail allergy in the same patients was due to cross-reactivity between hdm and snail allergenic components. however, the cross-reacting allergen(s) have not yet been identified. in vitro reactivity of seven patients' sera to the various extracts and hemolymph of four different helix snail species was analyzed by ige detection and immunodots and western blots. cross-reactivity between snails and d ... | 1998 | 9534913 |
| cross-reactivity between terrestrial snails (helix species) and house-dust mite (dermatophagoides pteronyssinus). i. in vivo study. | clinical reports have suggested an unusual frequency in the number of patients with food allergy to snails who are also allergic to the house-dust mite (hdm). as allergy to hdm is one of the most frequent sensitizations in atopic patients of western countries, evaluation of the relevance of the concomitant sensitization to dermatophagoides pteronyssinus and to snails is an important consideration. to evaluate the responsibility of different snail components and of snail mites for inducing in viv ... | 1998 | 9534912 |
| house dust mite antigen exposure of patients with atopic dermatitis of psoriasis. | we studied the amount of house dust antigen in the beds of 55 patients with atopic dermatitis, eleven patients with psoriasis and ten healthy volunteers using a commercial elisa which can determine the amount of antigen from dermatophagoides pteronyssinus, d. farinae and d. microseras expressed as nanogram (ng) antigen per gram of house dust. the world health organization has indicated that 10,000 ng house dust mite antigen per gram house dust can elicit an asthma attack in ige-sensitized patien ... | 1998 | 9534894 |
| asthma in the english-speaking caribbean. | the prevalence of asthma in the caribbean is high and seems to be increasing. asthma research in this region has been biased towards the paediatric population and there is little published on adult disease. there is a high prevalence of other allergic diseases in the patients studied, with skin reactivity to at least one allergen in 50-81% of subjects. reactivity to house dust mite, especially dermatophagoides pteronyssinus, occurred most commonly and sensitivity to this mite correlated with hig ... | 1998 | 10097663 |
| occurrence of mites in norway and the rest of scandinavia. | this paper gives a faunistic review of mites that have been recorded in the indoor environment, particularly homes, barns, and stored products, in norway and the other scandinavian countries. some preliminary results are given from unpublished investigations in norway. seven species of pyroglyphid mites have been recorded: dermatophagoides pteronyssinus, d. microceras, d. farinae, d. evansi, euroglyphus maynei, gymnoglyphus longior, and hirstia chelidonis. d. pteronyssinus was the predominant sp ... | 1998 | 10096804 |
| quantification of house-dust-mite populations. | since the proposal that allergen from the mite dermatophagoides pteronyssinus is identical to the house-dust allergen supposed to cause asthma and allergy, interest in the abundance, species diversity, and frequency of mites in the home has expanded. surveys and ecologic studies have confirmed the ubiquity of mites in house dust, especially mites of the family pyroglyphidae, but most do not give an accurate estimate of population size or mite exposure. these are still important issues today in a ... | 1998 | 10096802 |
| in vivo and ex vivo inhibitory effects of loratadine on histamine release in patients with allergic rhinitis. | the aim of this study was to evaluate the in vivo and ex vivo effects of the h1-antagonist loratadine on histamine release. | 1998 | 9930595 |