Publications

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galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: regulatory roles of cell surface glycans.galectin-1 is known to be one of the extracellular matrix proteins. to elucidate the biological roles of galectin-1 in cell adhesion and invasion of human anaplastic large cell lymphoma, we performed cell adhesion and invasion assays using the anaplastic large cell lymphoma cell line h-alcl, which was previously established in our laboratory. from the cell surface lectin array, treatment with neuraminidase from arthrobacter ureafaciens which cleaves all linkage types of cell surface sialic acid ...201424589677
lectin binding in meningiomas.forty-two meningiomas of different morphological sub-type were examined to determine their pattern of binding to 11 different lectins which characterize cell surface components such as carbohydrate residues. histiocytic and xanthoma cells within meningiomas could be demonstrated with six different lectins: wheat germ agglutinin (wga), peanut agglutinin (pna) bauhinia purpurea agglutinin (bpa), helix pomatia agglutinin (hpa), vicia fava agglutinin (vfa) and soyabean agglutinin (sba). vascular ele ...20153658105
degalactosylated/desialylated bovine colostrum induces macrophage phagocytic activity independently of inflammatory cytokine production.colostrum contains antibodies, such as immunoglobulin g (igg), immunoglobulin a (iga) and immunoglobulin m (igm), and, therefore, has potent immunomodulating activity. in particular, iga has an o-linked sugar chain similar to that in the group-specific component (gc) protein, a precursor of the gc protein-derived macrophage-activating factor (gcmaf). in the present study, we investigated the macrophage-activating effects of degalactosylated/desialylated bovine colostrum.201526168491
hydrastine pharmacokinetics and metabolism after a single oral dose of goldenseal (hydrastis canadensis) to humans.the disposition and metabolism of hydrastine was investigated in 11 healthy subjects following an oral dose of 2.7 g of goldenseal supplement containing 78 mg of hydrastine. serial blood samples were collected for 48 hours, and urine was collected for 24 hours. hydrastine serum and urine concentrations were determined by liquid chromatography-tandem mass spectrometry (lc-ms/ms). pharmacokinetic parameters for hydrastine were calculated using noncompartmental methods. the maximal serum concentrat ...201525609220
[synthesis and localization of l-lactate oxidase in yeasts].conditions for l-lactate oxidase synthesis by the yeast yarrowia lpolytica were investigated. the enzyme was found to be synthesized during growth on l-lactate in the exponential growth phase. l-lactate oxidase synthesis was observed, also on glucose after adaptation to stress conditions (oxidative or thermal stress) r during the stationary growth phase after glucose consumption. the cells grown on l-lactate exhibited high levels of antioxidant enzymes (catalase, superoxide dismutase, glucose-6- ...201525844463
penicillium ochrochloron mtcc 517 chitinase: an effective tool in commercial enzyme cocktail for production and regeneration of protoplasts from various fungi.penicillium ochrochloron mtcc 517 is a potent producer of chitinolytic enzymes. novozyme 234, traditional enzyme cocktail for protoplast generation is not available in the market. so, new enzyme cocktail is prepared for protoplast formation from various filamentous fungi which consists of 5 mg ml(-1) lysing enzymes from trichoderma harzianum, 0.06 mg ml(-1) β-glucuronidase from helix pomatia and 1 mg ml(-1) p. ochrochloron chitinase. the greatest number of protoplasts could be produced from most ...201525737658
pseudomonas aeruginosa arylsulfatase: a purified enzyme for the mild hydrolysis of steroid sulfates.the hydrolysis of sulfate ester conjugates is frequently required prior to analysis for a range of analytical techniques including gas chromatography-mass spectrometry (gc-ms). sulfate hydrolysis may be achieved with commercial crude arylsulfatase enzyme preparations such as that derived from helix pomatia but these contain additional enzyme activities such as glucuronidase, oxidase, and reductase that make them unsuitable for many analytical applications. strong acid can also be used to hydroly ...201525739756
the influence of electromagnetic pollution on living organisms: historical trends and forecasting changes.current technologies have become a source of omnipresent electromagnetic pollution from generated electromagnetic fields and resulting electromagnetic radiation. in many cases this pollution is much stronger than any natural sources of electromagnetic fields or radiation. the harm caused by this pollution is still open to question since there is no clear and definitive evidence of its negative influence on humans. this is despite the fact that extremely low frequency electromagnetic fields were ...201525811025
measuring faecal epi-androsterone as an indicator of gonadal activity in spotted hyenas (crocuta crocuta).enzyme immunoassays (eia) that measure faecal testosterone metabolites (ftm) are useful tools to monitor gonadal activity. the aim of this study was to validate an "in-house" epiandrosterone eia to monitor ftm in spotted hyenas. ftm were characterised in a male and a female hyena that each received an injection of 3h-testosterone. high-performance liquid chromatography (hplc) analyses revealed a cluster of highly polar enzyme-hydrolysable hormone metabolite conjugates. we performed hydrolysis us ...201526107516
molecular characterization of intergeneric hybrid between aspergillus oryzae and trichoderma harzianum by protoplast fusion.protoplast fusion between aspergillus oryzae and trichoderma harzianum and application of fusant in degradation of shellfish waste.201525444252
[a case of congenital type ii dyserythropoiesis. demonstration of the antigen with helix pomatia extracts. comparative study with antigens of the cad series]. 20154453773
structure and properties of hemocyanins. xii. electron microscopy of dissociation products of helix pomatia alpha-hemocyanin: quaternary structure. 20154453014
structure and properties of hemocyanins. xiv. reassociation of helix pomatia alpha-hemocyanin. 20154453008
lectin histochemistry shows wga, pha-l and hpa binding increases during progression of human colorectal cancer.most colorectal carcinomas develop from an adenoma-carcinoma sequence to metastatic disease. the aim of the present study was to use lectins, carbohydrate-binding proteins, to detect changes in glycosylation during this malignant progression.201526408694
praziquantel efficacy against brachylaima sp. metacercariae (trematoda: brachylaimidae) parasitizing the edible landsnail cornu aspersum and its hplc-ms/ms residue determination.cornu aspersum is a terrestrial edible snail, often parasitized by brachylaima (trematoda) metacercariae. ingestion of undercooked snails by humans allows metacercariae to develop to adult in the intestine causing brachylaimiasis (expected mortality rate 5-10%). the cosmopolitan character of the trematode and of its vector, enhanced in a future climate change scenario, and the absence of adequate sanitary controls of the snails in marketplaces clearly increase the risk of human brachylaimiasis. ...201526140831
accumulation and risk assessment of heavy metals in water, sediments, and aquatic organisms in rural rivers in the taihu lake region, china.concentrations of heavy metals (cd, cr, cu, ni, pb, and zn) were measured in water, sediments, ceratophyllum (hornwort), and bellamya sp. (edible snail) from residential, mixed (industrial and commercial), and agricultural areas with rural rivers in the taihu lake region, china. zn concentrations were the highest, whereas cd concentrations were the lowest among the six metals in water, sediments, and aquatic organisms. cd was mainly present in the acid-soluble fraction, cr in the residual fracti ...201525422112
pharmacological studies confirm neurotoxic metabolite(s) produced by the bloom-forming cylindrospermopsis raciborskii in hungary.a rapid cyanobacterial bloom of cylindrospermopsis raciborskii (3.2 × 10(4) filaments/ml) was detected early november, 2012, in the fancsika pond (east hungary). the strong discoloration of water was accompanied by a substantial fish mortality (even dead cats were seen on the site), raising the possibility of some toxic metabolites in the water produced by the bloom-forming cyanobacteria (c. raciborskii). the potential neuronal targets of the toxic substances in the bloom sample were studied on ...201524293352
[activators of protein kinase a and epac proteins enhance the contractile response of the isolated snail (helix pomatia) heart to serotonin].the mechanisms of camp action on the contractility of the isolated heart were studied in the snail helix pomatia. serotonin is a powerful activator of heart contractility in this animal. preincubation of the isolated heart ventricle with the activator of protein kinase a (pka) sp-8-bromoadenosine-3',5'-cyclic monophosphothioate (200 microm) or the activator of epac proteins 8-(4-chlorophenylthio)-2'-o-methyladenosine-3',5'-cyclic monophosphate (100 microm) proved to enhance the amplitude of cont ...201619198079
slow changes of membrane permeability in giant neurones of helix pomatia. 20165100571
purification of a -1,4-glucan hydrolase (cellulase) from the snail, helix pomatia. 20164714910
putative new groups of invertebrate water channels based on the snail helix pomatia l. (helicidae) mip protein identification and phylogenetic analysis.water channel proteins, classified as a family of membrane intrinsic proteins (mips) superfamily, enable rapid movement of water and small uncharged molecules through biological membranes. although water channel proteins are required in several important processes characteristic for the animals, such as osmoregulation, mucus secretion, or defense against desiccation, molluscs, until now, have been very poorly explored in this aspect. therefore, we decided to study mips in helix pomatia l. applie ...201627662803
phytoestrogens in milk: overestimations caused by contamination of the hydrolytic enzyme used during sample extraction.isoflavones are natural phytoestrogens with antioxidant and endocrine-disrupting potencies. monitoring of their levels is important to ensure the high quality and safety of food, milk, and dairy products. the efficiency and accuracy of phytoestrogen analyses in complex matrices such as milk depend on the extraction procedure, which often uses hydrolysis by means of the β-glucuronidase/sulfatase enzyme originating from helix pomatia. the present study reveals that the commercially available hydro ...201627394955
n-acetyl-l-cysteine and cysteamine as new strategies against mixed biofilms of nonencapsulated streptococcus pneumoniae and nontypeable haemophilus influenzae.acute otitis media, a polymicrobial disease of the middle ear cavity of children, is a significant public health problem worldwide. it is most frequently caused by encapsulated streptococcus pneumoniae and nontypeable haemophilus influenzae, although the widespread use of pneumococcal conjugate vaccines is apparently producing an increase in the carriage of nonencapsulated s. pneumoniae frequently, pneumococci and h. influenzae live together in the human nasopharynx, forming a self-produced biof ...201727919900
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