Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a method for field infestation with meloidogyne incognita. | a field inoculation method was developed to produce meloidogyne spp. infestation sites with minimal quantities of nematode inoculum and with a reduced labor requirement compared to previous techniques. in a preseason-methyl bromidefumigated site, nematode egg suspensions were delivered at concentrations of 0 or 10(x) eggs/m of row where x = 2.12, 2.82, 3.52, or 4.22 through a drip line attached to the seed firmer of a commercial 2-row planter into the open seed furrow while planting cowpea. thes ... | 2005 | 19262898 |
| use of cucumis metuliferus as a rootstock for melon to manage meloidogyne incognita. | root-knot nematode-susceptible melons (cantaloupe) were grown in pots with varying levels of meloidogyne incognita and were compared to susceptible melons that were grafted onto cucumis metuliferus or cucurbita moschata rootstocks. in addition, the effect of using melons as transplants in nematode-infested soil was compared to direct seeding of melons in nematode-infested soil. there were no differences in shoot or root weight, or severity of root galling between transplanted and direct-seeded n ... | 2005 | 19262873 |
| application of rna interference to root-knot nematode genes encoding esophageal gland proteins. | plant parasitic nematodes have been, so far, refractory to transformation or mutagenesis. the functional analysis of nematode genes relies on the development of reverse genetic tools adapted to these obligate parasites. here, we describe the application of rna interference (rnai) to the root-knot nematode meloidogyne incognita for the knock-down of two genes expressed in the subventral esophageal glands of the nematode and potentially involved in parasitism, the calreticulin (mi-crt) and the pol ... | 2005 | 16042006 |
| nematicidal natural products from the aerial parts of lantana camara linn. | lantanilic acid, camaric acid and oleanolic acid possessing nematicidal activity were isolated from the methanolic extract of the aerial parts of lantana camara linn. through bio-assay guided fractionation. these compounds exhibited 98%, 95% and 70% mortality respectively against root-knot nematode meloidogyne incognita at 0.5% concentration. conventional nematicide furadan showed 100% mortality at this concentration. | 2005 | 16010828 |
| isolation and characterization of a cdna encoding a serine proteinase from the root-knot nematode meloidogyne incognita. | this report describes the first serine proteinase gene isolated from the sedentary nematode meloidogyne incognita. using degenerate primers, a 1372bp cdna encoding a chymotrypsin-like serine proteinase (mi-ser1) was amplified from total rna of adult females by rt-pcr and 5' and 3' rapid amplification of cdna ends. the deduced amino acid sequence of mi-ser1 encoded a putative signal peptide and a prodomain of 22 and 33 amino acids, respectively, and a mature proteinase of 341 amino acids with a p ... | 2005 | 15888294 |
| genome-wide expression profiling of the host response to root-knot nematode infection in arabidopsis. | during a compatible interaction, root-knot nematodes (meloidogyne spp.) induce the redifferentiation of root cells into multinucleate nematode feeding cells (giant cells). hyperplasia and hypertrophy of the surrounding cells leads to the formation of a root gall. we investigated the plant response to root-knot nematodes by carrying out a global analysis of gene expression during gall formation in arabidopsis, using giant cell-enriched root tissues. among 22 089 genes monitored with the complete ... | 2005 | 16236154 |
| pineappledb: an online pineapple bioinformatics resource. | a world first pineapple est sequencing program has been undertaken to investigate genes expressed during non-climacteric fruit ripening and the nematode-plant interaction during root infection. very little is known of how non-climacteric fruit ripening is controlled or of the molecular basis of the nematode-plant interaction. pineappledb was developed to provide the research community with access to a curated bioinformatics resource housing the fruit, root and nematode infected gall expressed se ... | 2005 | 16202174 |
| effects of jasmonate-induced defenses on root-knot nematode infection of resistant and susceptible tomato cultivars. | jasmonates, such as jasmonic acid (ja), are plant-signaling compounds that trigger induced resistance against certain pathogens and a broad range of arthropod herbivores. one goal of this study was to determine the effects of ja-dependent defenses in tomato on root-knot nematodes. another was to determine if the artificial induction of these defenses could enhance nematode control on plants that carry mi-1.2, a nematode resistance gene that is present in many tomato cultivars. at moderate soil t ... | 2005 | 16132206 |
| morphological and molecular characterization of a new root-knot nematode, meloidogyne thailandica n. sp. (nematoda: meloidogynidae), parasitizing ginger (zingiber sp.). | a root-knot nematode meloidogyne thailandica n. sp. was discovered on roots of ginger (zingiber spp.) intercepted from thailand in october 2002 by the u.s. department of agriculture animal and plant health inspection service at the port of san francisco. comparison by light microscopy (lm) and scanning electron microscopy (sem) to five other morphologically related species (m. incognita, m. arenaria, m. microcephala, m. megatyla, and m. enterolobii) revealed that the new species differs from the ... | 2005 | 19262883 |
| laser capture microdissection and real-time pcr for measuring mrna in giant cells induced by meloidogyne javanica. | the techniques of laser capture microdissection and quantitative rt-pcr were investigated as methods for measuring mrna in giant cells induced by meloidogyne javanica. laser capture microdissection allowed precise sampling of giant cells at 1 to 3 weeks after inoculation. the expression of three genes (a water channel protein gene rb7, a plasma membrane h(+)-atpase (lha4), and a hexose kinase (hxk1) was measured based on mrna extracted from tissue samples and quantitated using reversetranscripti ... | 2005 | 19262878 |
| effects of application strategies of fumigant and nonfumigant nematicides on cantaloupe grown in deep sand soils in florida. | a 2-year study was conducted in which three treatment tactics of oxamyl (at planting application, application every 2 weeks, and rescue applications, as determined by crop symptoms) were compared to fumigant treatments with methyl bromide, 1,3-dichloropropene (1,3-d), and 1,3-d plus chloropicrin for management of meloidogyne spp. in 2002, treatments that included 1,3-d produced higher yields as determined both by number and weight of marketable fruit. all treatment tactics relying solely on oxam ... | 2005 | 19262874 |
| studies on the efficacy of the antihelminthic antibiotic (kt-199) in the control of root-knot nematode meloidogyne incognita (kofoid and white) chitwood on tomato. | 2005 | 6746308 | |
| in planta secretion of a calreticulin by migratory and sedentary stages of root-knot nematode. | esophageal secretions from endoparasitic sedentary nematodes are thought to play key roles throughout plant parasitism, in particular during the invasion of the root tissue and the initiation and maintenance of the nematode feeding site (nfs) essential for nematode development. the secretion in planta of esophageal cell-wall-degrading enzymes by migratory juveniles has been shown, suggesting a role for these enzymes in the invasion phase. nevertheless, the secretion of an esophageal gland protei ... | 2005 | 16478047 |
| conserved and variable domains in satellite dnas of mitotic parthenogenetic root-knot nematode species. | two satellite dnas have been characterized in the mitotic parthenogenetic root-knot nematodes meloidogyne javanica and m. paranaensis, agriculturally important phytoparasitic species. the satellite repeat variants cloned from m. javanica could not be resolved from those described earlier in m. arenaria [castagnone-sereno, p., leroy, f., abad, p., 2000. cloning and characterization of an extremely conserved satellite dna family from the root-knot nematode meloidogyne arenaria. genome 43, 346-353] ... | 2005 | 16229973 |
| a recessive gene for resistance to meloidogyne arenaria in interspecific arachis spp. hybrids. | a single dominant gene for resistance to meloidogyne arenaria was identified previously in two peanut cultivars, arachis hypogaea 'coan' and 'nematam'. the interspecific arachis hybrid txag-6 was the source of this resistance and the donor parent in a backcross breeding program to introgress resistance into cultivated peanut. to determine if other resistance genes were present in txag-6 and derived breeding populations from the third backcross generation (bc), f individuals were evaluated for th ... | 2005 | 19262858 |
| preliminary study of the green algae chlorella (chlorella vulgaris) for control on the root-knot nematode (meloidogyne arenaria) in tomato plants and ectoparasite xiphinema indexin grape seedlings. | the alternative ecological methods require investigation of many organo-biological means for plant protection against dangerous root parasites such as root-knot nematode meloidogyne arenaria and some ectoparasites (xiphinema index). the bulgarian organic product - dry extract of green alga chlorella vulgaris ("the golden apple"-plamen barakov) is the latest product, which in comparative aspect gives the best results. series of laboratory and pot experiments are carried out with tomato (cv. bele ... | 2005 | 16628939 |
| two chorismate mutase genes from the root-knot nematode meloidogyne incognita. | summary parasitism genes encoding secretory proteins expressed in the oesophageal glands of phytoparasitic nematodes play critical roles in nematode invasion of host plants, establishment of feeding sites and suppression of host defences. two chorismate mutase (cm) genes potentially having a role in one or more of these processes were identified from a meloidogyne incognita oesophageal gland-cell subtractive cdna library. these m. incognita enzymes (designated as mi-cm-1 and mi-cm-2) with amino- ... | 2005 | 20565635 |
| internalization of escherichia coli o157:h7 following biological and mechanical disruption of growing spinach plants. | the internalization and persistence of a bioluminescent escherichia coli o157:h7 ph1 was investigated in growing spinach plants that had been either biologically or mechanically damaged. in control (undamaged) plants cultivated in soil microcosms inoculated with e. coli o157:h7 phl, the bacterium was recovered from surface-sterilized root tissue but not from leaves. mechanical disruption of the seminal root and root hairs of the plants did not result in the internalization of the pathogen into t ... | 2005 | 16355819 |
| development of pasteuria penetrans in meloidogyne javanica females as affected by constantly high vs fluctuating temperature in an in-vivo system. | growth room and glasshouse experiment was conducted to investigate the effect of constant and fluctuating temperatures on the development of pasteuria penetrans a hyperparasite of root-knot nematodes. tomato plants (lycopersicon esculentum mill) were inoculated with meloidogyne javanica second-stage juveniles attached with endospores of p. penetrans and were grown in growth room at 26-29 degrees c and in glasshouse at 20-32 degrees c. the tomato plants were sampled from the growth room after 600 ... | 2005 | 15682497 |
| the effect of different initial densities of nematode (meloidogyne javanica) on the build-up of pasteuria penetrans population. | pasteuria penetrans will build-up faster where there is a high initial nematode density and can suppress root-knot nematode populations in the roots of tomato plants. the effect of different initial densities of nematode (meloidogyne javanica) (150, 750, 1500, 3000) and p. penetrans infected females (f1, f3) densities (f0=control and ac=absolute control without nematode or p. penetrans inoculum) on the build-up of pasteuria population was investigated over four crop cycles. two major points of i ... | 2005 | 15633246 |
| phenylacetic acid-producing rhizoctonia solani represses the biosynthesis of nematicidal compounds in vitro and influences biocontrol of meloidogyne incognita in tomato by pseudomonas fluorescens strain cha0 and its gm derivatives. | the aim of the present investigation was to determine the influence of rhizoctonia solani and its pathogenicity factor on the production of nematicidal agent(s) by pseudomonas fluorescens strain cha0 and its gm derivatives in vitro and nematode biocontrol potential by bacterial inoculants in tomato. | 2005 | 15610416 |
| high-nitrogen compost as a medium for organic container-grown crops. | compost was tested as a medium for organic container-grown crops. nitrogen (n) loss during composting of separated cow manure (scm) was minimized using high c/n (wheat straw, ws; grape marc, gm) or a slightly acidic (orange peels, op) additives. n conservation values in the resultant composts were 82%, 95% and 98% for gm-scm, op-scm and ws-scm, respectively. physical characteristics of the composts were compatible with use as growing media. the nutritional contribution of the composts was assess ... | 2005 | 15491822 |
| extracellular protease of pseudomonas fluorescens cha0, a biocontrol factor with activity against the root-knot nematode meloidogyne incognita. | in pseudomonas fluorescens cha0, mutation of the gaca-controlled apra gene (encoding the major extracellular protease) or the gaca regulatory gene resulted in reduced biocontrol activity against the root-knot nematode meloidogyne incognita during tomato and soybean infection. culture supernatants of strain cha0 inhibited egg hatching and induced mortality of m. incognita juveniles more strongly than did supernatants of apra and gaca mutants, suggesting that apra protease contributes to biocontro ... | 2005 | 16151170 |
| phylogenetic analysis of pasteuria penetrans by use of multiple genetic loci. | pasteuria penetrans is a gram-positive, endospore-forming eubacterium that apparently is a member of the bacillus-clostridium clade. it is an obligate parasite of root knot nematodes (meloidogyne spp.) and preferentially grows on the developing ovaries, inhibiting reproduction. root knot nematodes are devastating root pests of economically important crop plants and are difficult to control. consequently, p. penetrans has long been recognized as a potential biocontrol agent for root knot nematode ... | 2005 | 16077116 |
| nematicidal activity and chemical component of poria cocos. | poria cocos, a famous traditional chinese medicine, was found to have nematicidal activity in experiments searching for nematicidal fungi. the experiment showed it could kill 94.9% of the saprophytic nematode, panagrellus redivivue, 92.6% of the root-knot nematode, meloidogyne arenaria, and 93.5% of the pine nematode, bursaphelenchus xylophilus, on pda plate within 12 hours. according to the nematicidal activity, three new compounds, 2, 4, 6-triacetylenic octane diacid, 2, 4, 5, 6-tetrahydroxyhe ... | 2005 | 15765052 |
| a novel approach to identify plant parasitic nematodes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | plant parasitic nematodes are difficult to identify because different species are morphologically similar, and this makes their control more difficult. the aim of this work was to develop a rapid, simple method to identify plant parasitic nematodes, based on analysis of protein profiles of nematodes generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms). two methods have been used: grinding and direct analysis of intact nematodes. both methods wer ... | 2005 | 15880621 |
| suppression of meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria. | four rhizobacteria selected out of over 500 isolates from rhizosphere of the vegetables in china were further studied for suppression of the root-knot nematode and soil-borne fungal pathogens in laboratory and greenhouse in belgium. they were identified as brevibacillus brevis or bacillus subtilis by biolog test and partial 16s rdna sequence comparison. they not only inhibited the radial growth of the root-infecting fungi rhizoctonia solani sx-6, pythium aphanidermatum zjp-1 and fusarium oxyspor ... | 2005 | 15909333 |
| studies on the management of root-knot nematode, meloidogyne incognita-wilt fungus, fusarium oxysporum disease complex of green gram, vigna radiata cv ml-1108. | studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (azadirachta indica) seed powder at 50 mg/kg soil, green mould (trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, meloidogyne incognita-wilt fungus, fusarium oxysporum disease complex on green gram, vigna radiata cv ml-1108. all the treatments significantly improved ... | 2005 | 16052706 |
| biological activity of spatoglossum asperum: a brown alga. | chloroform and methanol fractions of an ethanol extract of spatoglossum asperum showed antifungal activity against the highly destructive plant pathogen, macrophomina phaseolina while the n-hexane fraction showed activity against rhizoctonia solani and fusarium solani. n-hexane and methanol fractions also showed nematicidal activity against the plant parasitic nematode, meloidogyne javanica. column chromatography of the ethanol extract of seaweed yielded oily fractions named sa-i to sa-vi. gc-ms ... | 2005 | 16161033 |
| variability of meloidogyne exigua on coffee in the zona da mata of minas gerais state, brazil. | minas gerais is the major coffee-producing state of brazil, with 28% of its production coming from the region of zona da mata. four major species of root-knot nematode attacking coffee (meloidogyne incognita, m. paranaensis, m. coffeicola, and m. exigua) have been reported from brazil. to determine the variability in meloidogyne spp. occurring in that region, 57 populations from 20 localities were evaluated for morphological, enzymatic, and physiological characteristics. according to the perinea ... | 2005 | 19262880 |
| esterase polymorphism in meloidogyne konaensis. | the continual detection of a slow (i1) esterase band in greenhouse cultures of meloidogyne konaensis isolated from the field led to a hypothesis that the nematode may be polymorphic for esterase. a survey of coffee fields demonstrated at least four esterase phenotypes were present in meloidogyne recovered. an f1 phenotype predominated (60% of the females), but an i1 phenotype was also common (30% of samples). a series of greenhouse and laboratory experiments were undertaken to understand this po ... | 2005 | 19262888 |
| effect of soils from six management systems on root-knot nematodes and plant growth in greenhouse assays. | the effects of soil management systems on root-knot nematode (meloidogyne incognita) eggs and gall incidence on tomato (lycopersicon esculentum) and cucumber (cucumis sativus) following tomato were evaluated. soil was collected from a replicated field experiment in which six management systems were being assessed for vegetable production. soil management systems were conventional production, organic production, bahiagrass (paspalum notatum) pasture, bahiagrass: stylosanthes (stylosanthes guianen ... | 2005 | 19262892 |
| study on infection symptoms of root-knot nematode, meloidogyneja vanica, on the stem of the tomato seedlings in greenhouse. | on the first of seeds germination, meloidogyne javanica induced knots on the stem eventually by to penetrate into the coleoptiles. in this experiment, used soil was loamy-sandy type, ph=7.2, including 1.5% composts. tomato seedlings of cultivar rutgers were used as host. this experiment was conducted in completely randomized design with 3 treatments and 10 replicates as fallow: (1) control, (2) 10 j2s/cm3 of soil, (3) 20 j2s/cm3 of soil. in each pot (1000 cm3), 50 seeds were planted. inoculation ... | 2005 | 16628935 |
| biological control potential of the obligate parasite pasteuria penetransagainst the root-knot nematode, meloidogyne incognita infestation in brinjal. | the efficacy of the obligate bacterial parasite, pasteuria penetrans against the rootknot nematode, meloidogyne incognita infestation was assessed in brinjal. the seedling pans with sterilized soil were inoculated with nematodes and root powder of p. penetrans were applied at different dosages viz., 0 x 10(6), 0.5 x 10(6) spores and 1 x 10(6) spores/pan. seeds of brinjal cv co2 were sown in the pans and seedlings were allowed to grow. the seedlings were transplanted to microplots containing ster ... | 2005 | 16628937 |
| integrated management of root-knot nematode, meloidogyne incognita infestation in tomato and grapevine. | an integrated approach with the obligate bacterial parasite, pasteuria penetrans and nematicides was assessed for the management of the root-knot nematode, meloidogyne incognita infestation in tomato and grapevine. seedlings of tomato cv. co3 were transplanted into pots filled with sterilized soil and inoculated with nematodes (5000 juveniles/pot). the root powder of p. penetrans at 10 mg/pot was applied alone and in combination with carbofuran at 6 mg/pot. application of p. penetrans along with ... | 2005 | 16628938 |
| impact of the nematophagous fungus pochonia chlamydosporia on nematode and microbial populations. | the microbial and nematode populations associated with two plants (tomato and cabbage) inoculated with the nematophagous fungus, pochonia chlamydosporia var. chlamydosporia or root knot nematode (meloidogyne incognita), or both, were compared with those in unplanted controls. the dominant factor affecting culturable microbial populations was found to be the presence or absence of tomato plants. generally microbial colony counts were lowest in unplanted soil, small increases were associated with ... | 2005 | 16363363 |
| distribution and downward movement of pasteuria penetrans in field soil. | endospores of pasteuria penetrans were evaluated for their vertical distribution in field soil and their downward movement through soil in the laboratory. in the field trial, the number of endospores attached to second-stage juveniles (j2) of meloidogyne arenaria race 1 varied greatly in different soil depths. there were higher percentages of j2 with endospores attached in former weed fallow plots during the first 3 years of growing peanut than in former bahiagrass and rhizomal peanut plots (p < ... | 2005 | 19262855 |
| morphological variability and molecular phylogeny of the nematophagous fungus monacrosporium drechsleri. | an isolate of the nematode-trapping fungus monacrosporium drechsleri was collected from cultures of the root-knot nematode meloidogyne arenaria that had been maintained on tomato roots in greenhouse pots in beltsville, maryland. the plant-parasitic nematodes heterodera glycines, meloidogyne incognita and pratylenchus zeae and the free-living nematodes caenorhabditis elegans and panagrellus redivivus were placed on colonies of m. drechsleri grown in petri dishes to study ability of the isolate to ... | 2005 | 16396348 |
| effects of fly ash, pseudomonas striata and rhizobium on the reproduction of nematode meloidogyne incognita and on the growth and transpiration of pea. | glasshouse experiments were conducted twice to assess the ash amendments (0, 20, and 40% with soil), a phosphate solubilizing microorganism pseudomonas striata and a root-nodule bacterium rhizobium sp on the reproduction of root-knot nematode meloidogyne incognita and on the growth and transpiration of pea. amendments of fly ash with soil had no effect on transpiration. however, m. incognita reduced the rate of transpiration from 1st week onward after inoculation while inoculation of rhizobium s ... | 2005 | 16114471 |
| tropical soil microflora of spice-based cropping systems as potential antagonists of root-knot nematodes. | suppression of plant parasitic nematodes with nematode predators, parasites or antagonists is an eco-friendly approach than the toxic chemicals. in a study, soil borne fungi from the rhizosphere of major spice crops were collected from diverse cropping systems prevailing in three southern states of india. a series of in vitro studies were conducted using 73 freshly collected fungal isolates and 76 isolates obtained from other sources. out of this 67 isolates were not parasitic on females of root ... | 2005 | 15955340 |
| root-knot nematode problem of some winter ornamental plants and its biomanagement. | a microplot study under field conditions was carried out during 2 consecutive years to assess the effect of root-knot nematode infection (2,000 meloidogyne incognita eggs/kg soil) on three winter ornamental plants: hollyhock (althea rosea), petunia (petunia hybrida), and poppy (papaver rhoeas). effects of root-dip treatment with the biocontrol agents pochonia chlamydosporia, bacillus subtilis, and pseudomonas fluorescens and the nematicide fenamiphos were tested. the three ornamental species wer ... | 2005 | 19262861 |
| reproduction of belonolaimus longicaudatus, meloidogyne javanica, paratrichodorus minor, and pratylenchus brachyurus on pearl millet (pennisetum glaucum). | pearl millet (pennisetum glaucum) has potential as a grain crop for dryland crop production in the southeastern united states. whether or not pearl millet will be compatible in rotation with cotton (gossypium hirsutum), corn (zea mays), and peanut (arachis hypogaea) will depend, in part, on its host status for important plant-parasitic nematodes of these crops. the pearl millet hybrid 'tifgrain 102' is resistant to both meloidogyne incognita race 3 and m. arenaria race 1; however, its host statu ... | 2005 | 19262863 |
| effect of three plant residues and chicken manure used as biofumigants at three temperatures on meloidogyne incognita infestation of tomato in greenhouse experiments. | plant residues of broccoli, melon, and tomato with or without addition of chicken manure were used as biofumigants in two pot experiments with meloidogyne incognita-infested soils. the efficacy of these biofumigants in controlling m. incognita infestation in susceptible tomato bio-assay plants was studied at soil temperatures of 20 masculine, 25 masculine, and 30 masculinec. none of the plant residues was effective at 20 masculinec, and broccoli was more effective than tomato or melon at 25 masc ... | 2005 | 19262896 |
| entomopathogenic nematodes and bacteria applications for control of the pecan root-knot nematode, meloidogyne partityla, in the greenhouse. | meloidogyne partityla is a parasite of pecan and walnut. our objective was to determine interactions between the entomopathogenic nematode-bacterium complex and m. partityla. specifically, we investigated suppressive effects of steinernema feltiae (strain sn) and s. riobrave (strain 7-12) applied as infective juveniles and in infected host insects, as well as application of s. feltiae's bacterial symbiont xenorhabdus bovienii on m. partityla. in two separate greenhouse trials, the treatments wer ... | 2006 | 19259462 |
| effects of inducers of systemic acquired resistance on reproduction of meloidogyne javanica and rotylenchulus reniformis in pineapple. | the potency of the inducers of systemic acquired resistance (sar), acibenzolar-s-methyl, dl-alpha-amino-n-butyric acid (aaba), dl-beta-amino-n-butyric acid (baba), gamma-amino-n-butyric acid (gaba), p-aminobenzoic acid (paba), riboflavin, and salicylic acid (sa), in reducing reproduction of meloidogyne javanica and rotylenchulus reniformis in pineapple was investigated. all inducers were applied as foliar sprays to 1-mon-old pineapple plants (20 ml/plant) grown in 22-cm-diam. pots in the greenho ... | 2006 | 19259535 |
| plantago lanceolata and plantago rugelii extracts are toxic to meloidogyne incognita but not to certain microbes. | extracts from the plants plantago lanceolata and p. rugelii were evaluated for toxicity to the root-knot nematode meloidogyne incognita, the beneficial microbes enterobacter cloacae, pseudomonas fluorescens and trichoderma virens, and the plant-pathogenic fungi fusarium oxysporum f. sp. gladioli, phytophthora capsici, pythium ultimum, and rhizoctonia solani. wild plants were collected, roots were excised from shoots, and the plant parts were dried and ground to a powder. one set of extracts (10% ... | 2006 | 19259537 |
| expression and regulation of the arabidopsis thaliana cel1 endo 1,4 beta glucanase gene during compatible plant-nematode interactions. | the root-knot nematode meloidogyne incognita is an obligate endoparasite of plant roots and stimulates elaborate modifications of selected root vascular cells to form giant cells for feeding. an arabidopsis thaliana endoglucanase (atcel1) promoter is activated in giant cells that were formed in atcel1::uida transgenic tobacco and arabidopsis plants. activity of the full-length atcel1 promoter was detected in root and shoot elongation zones and in the lateral root primordia. different 5' and inte ... | 2006 | 19259541 |
| isolation and characterization of another cdna encoding a chorismate mutase from the phytoparasitic nematode meloidogyne arenaria. | a new cdna, named ma-cm-2, encoding a chorismate mutase (cm), has been isolated from meloidogyne arenaria. the full-length cdna, carrying the trans-spliced sl1 leader sequence, was 753-bp long with an open reading frame of 576 bp. the deduced protein ma-cm-2 including amino-terminal signal peptide shows significant similarity to cms of meloidogyne incognita, meloidogyne javanica, and also bacteria. secondary structure prediction of ma-cm-2 indicates the presence of the three conserved alpha-heli ... | 2006 | 16458297 |
| a method for isolation of pasteuria penetrans endospores for bioassay and genomic studies. | a rapid method for collection of pasteuria penetrans endospores was developed. roots containing p. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. this method obviates laborious handpicking of pasteuria-infected females and yields endospores competent to attach to and infect nematodes. endospores are suitable for morphology studies and dna preparations. | 2006 | 19259442 |
| influence of lysobacter enzymogenes strain c3 on nematodes. | chitinolytic microflora may contribute to biological control of plant-parasitic nematodes by causing decreased egg viability through degradation of egg shells. here, the influence of lysobacter enzymogenes strain c3 on caenorhabditis elegans, heterodera schachtii, meloidogyne javanica, pratylenchus penetrans, and aphelenchoides fragariae is described. exposure of c. elegans to l. enzymogenes strain c3 on agar resulted in almost complete elimination of egg production and death of 94% of hatched j ... | 2006 | 19259452 |
| a fusarium wilt resistance gene in gossypium barbadense and its effect on root-knot nematode-wilt disease complex. | abstract fusarium wilt, caused by the soilborne pathogen fusarium oxysporum f. sp. vasinfectum race 1, is a vascular disease in cotton (gossypium spp.), and is a component of a disease complex with root-knot nematodes (meloidogyne incognita). genetic analysis of two interspecific crosses (g. barbadense pima s-7 x g. hirsutum acala nemx and pima s-7 x acala sj-2) showed that one major gene (designated fov1) with allele dosage effect conferred resistance to f. oxysporum f. sp. vasinfectum race 1 i ... | 2006 | 18943146 |
| use of trichoderma harzianum and trichoderma viride for the biological control of meloidogyne incognita on tomato. | trichoderma harzianum and trichoderma viride were tested for their capacity to reduce the incidence of the root-knot nematode meloidogyne incognita on tomato. in vitro studies demonstrated that all tested isolates were effective in causing nematode mortality compared with the control. trichoderma slightly reduced nematode damage to tomato in vivo. treatment of the soil with the biocontrol agents before transplanting, improved control over treatment directly at transplanting. the trichoderma isol ... | 2006 | 17390844 |
| colonization by the arbuscular mycorrhizal fungus glomus versiforme induces a defense response against the root-knot nematode meloidogyne incognita in the grapevine (vitis amurensis rupr.), which includes transcriptional activation of the class iii chitinase gene vch3. | inoculation of the grapevine (vitis amurensis rupr.) with the arbuscular mycorrhizal (am) fungus glomus versiforme significantly increased resistance against the root-knot nematode (rkn) meloidogyne incognita. studies using relative quantitative reverse transcription-pcr (rqrt-pcr) analysis of grapevine root inoculation with the am fungus revealed an up-regulation of vch3 transcripts. this increase was greater than that observed following infection with rkn. however, inoculation of the mycorrhiz ... | 2006 | 16326755 |
| a root-knot nematode secretory peptide functions as a ligand for a plant transcription factor. | parasitism genes expressed in the esophageal gland cells of root-knot nematodes encode proteins that are secreted into host root cells to transform the recipient cells into enlarged multinucleate feeding cells called giant-cells. expression of a root-knot nematode parasitism gene which encodes a novel 13-amino-acid secretory peptide in plant tissues stimulated root growth. two scarecrow-like transcription factors of the gras protein family were identified as the putative targets for this bioacti ... | 2006 | 16673933 |
| nematode infection and reproduction in transgenic and mutant arabidopsis and tobacco with an altered phenylpropanoid metabolism. | transgenic and mutant arabidopsis and tobacco plants with altered phenylpropanoid metabolism were infected with the plant parasitic root knot nematode meloidogyne incognita to assess the effect of the transgene or mutation on nematode infection and reproduction. modifications in the lignin biosynthetic pathway which alter lignin composition in roots affected reproduction. in arabidopsis with increased levels of syringyl lignin, reproduction was lower than in wild-type plants, while in tobacco wi ... | 2006 | 16831845 |
| [diversity of actinomycetes associated with root-knot nematode and their potential for nematode control]. | twenty actinomycetes were isolated from root-knot nematode eggs and females collected from 11 plant root samples infested by meloidogyne spp.. the isolates were assigned to the genera streptomyces, nocardia and pseudonocardia respectively, based on analysis of morphological characteristics, cell-wall daps and 16s rrna gene sequences. 80% of them were streptomycetes. biocontrol potential of the isolates against meloidogyne hapla was evaluated in liquid culture in vitro. the average percentages of ... | 2006 | 17037062 |
| integrated application of some compatible biocontrol agents along with mustard oil seed cake and furadan on meloidogyne incognita infecting tomato plants. | experiments were carried out to study the effect of two fungal bioagents along with mustard oil cake and furadan against root knot nematode meloidogyne incognita infecting tomato under greenhouse condition. bioagents viz., paecilomyces lilacinus and trichoderma viride alone or in combination with mustard cake and furadan promoted plant growth, reduced number of galls/plant, egg masses/root system and eggs/egg mass. the fungal bioagents along with mustard cake and nematicide showed least nematode ... | 2006 | 17048300 |
| sensitive pcr detection of meloidogyne arenaria, m. incognita, and m. javanica extracted from soil. | we have developed a simple pcr assay protocol for detection of the root-knot nematode (rkn) species meloidogyne arenaria, m. incognita, and m. javanica extracted from soil. nematodes are extracted from soil using baermann funnels and centrifugal flotation. the nematode-containing fraction is then digested with proteinase k, and a pcr assay is carried out with primers specific for this group of rkn and with universal primers spanning the its of rrna genes. the presence of rkn j2 can be detected a ... | 2006 | 19259460 |
| low-temperature scanning electron microscopy in fungus-nematode interaction. | a comparative study has been made of different preparation techniques used for the scanning electron microscope (sem), with regard to their application to fungus-nematode interaction. the preparation of frozen-hydrated specimens of both healthy and arthrobotrys-oligospora-infected second-stage larvae of the root-knot nematode (meloidogyne sp.) is described, and the results are compared with those obtained by critical point-drying and freeze-drying. in all cases the frozen-hydrated specimens cons ... | 2006 | 8281360 |
| damage and management of meloidogyne hapla using oxamyl on carrot in new york. | the northern root-knot nematode (meloidogyne hapla) is a major pathogen of processing carrot in new york, significantly reducing marketable yield and profitability. severely infected carrots are stubby, galled and forked and therefore unmarketable. in field microplot trials in 1996 and 1998, the incidence and severity of root-galling increased and the marketable yield of carrot decreased as the initial inoculum density of m. hapla was increased from 0 to 8 eggs/cm(3) soil, in mineral or organic ... | 2006 | 19259467 |
| screening of field pea (pisum sativum) selections for their reactions to root-knot nematode (meloidogyne incognita). | pot studies were carried out to evaluate the reactions of 23 selections of field pea (pisum sativum) against meloidogyne incognita (2000 freshly hatched juveniles (j2)/pot). experiment was conducted under greenhouse conditions ((24.7+/-3) degrees c and (62+/-7)% rh) and terminated 45 d after inoculation. the roots of all the selections were assessed to determine root-knot indices (rki) on a 0-4 scale. out of 23 selections hfp-990713, pant p-25, and hfp-0129 were resistant; pant p-2005, ndp-2 and ... | 2006 | 16502508 |
| qtl mapping for resistance to root-knot nematodes in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source. | root-knot nematodes meloidogyne incognita (kofoid and white) can cause severe yield loss in cotton (gossypium hirsutum l.). the objectives of this study were to determine the inheritance and genomic location of genes conferring root-knot nematode resistance in m-120 rnr, a highly resistant g. hirsutum line with the auburn 623 rnr source of resistance. utilizing two interspecific f(2) populations developed from the same m-120 rnr by gossypium barbadense (cv. pima s-6) cross, genome-wide scanning ... | 2006 | 16960714 |
| phenotypic expression of rkn1-mediated meloidogyne incognita resistance in gossypium hirsutum populations. | the root-knot nematode meloidogyne incognita is a damaging pest of cotton (gossypium hirsutum) worldwide. a major gene (rkn1) conferring resistance to m. incognita was previously identified on linkage group a03 in g. hirsutum cv. acala nemx. to determine the patterns of segregation and phenotypic expression of rkn1, f(1), f(2), f(2:3), bc(1)f(1) and f(2:7) recombinant inbred lines (ril) from intraspecific crosses between acala nemx and a closely related susceptible cultivar acala sj-2 were inocu ... | 2006 | 19259455 |
| a symbiont-independent endo-1,4-beta-xylanase from the plant-parasitic nematode meloidogyne incognita. | substituted xylan polymers constitute a major part of the hemicellulose fraction of plant cell walls, especially in monocotyledons. endo-1,4-beta-xylanases (ec 3.2.1.8) are capable of hydrolyzing substituted xylan polymers into fragments of random size. many herbivorous animals have evolved intimate relationships with endosymbionts to exploit their enzyme complexes for the degradation of xylan. here, we report the first finding of a functional endo-1,4-beta-xylanase gene from an animal. the gene ... | 2006 | 16673939 |
| soil amendment with dried weed leaves as non-chemical approach for the management of meloidogyne incognita infecting tomato. | in pot trial, dried ground weed leaves of cynodon dactylon, datura stramonium, eichhomia crassipes, emex spinosus, ricinus communis and sisymbrium irio were mixed with soil at the rate of 1, 3, 5 and 10 g/kg soil and compared their nematicidal potential with carbofuran as a standard against the root-knot nematode, m. incognita infecting tomato. in addition, their effects on growth rate of tomato plants were also investigated. the results showed that m. incognita populations in the soil and root ... | 2006 | 17612348 |
| characterization of a new beta-1,4-endoglucanase gene from the root-knot nematode meloidogyne incognita and evolutionary scheme for phytonematode family 5 glycosyl hydrolases. | cellulases from plant parasitic nematodes are encoded by multiple gene families and are thought to originate from horizontal gene transfer. unraveling the evolution of these genes in the phylum will help understanding the evolution of plant parasitism in nematodes. here we describe a new gene, named mi-eng-2, that encodes a family 5 glycosyl hydrolase (ghf5) with a predicted signal peptide and devoid of linker domain and cellulose-binding domain. the beta-1,4-endoglucanase activity of the protei ... | 2006 | 16962258 |
| identification and mapping of microsatellite markers linked to a root-knot nematode resistance gene (rkn1) in acala nemx cotton (gossypium hirsutum l.). | host-plant resistance is the most economic and effective strategy for root-knot nematode (rkn) meloidogyne incognita control in cotton (gossypium hirsutum l.). molecular markers linked to resistance are important for incorporating resistance genes into elite cultivars. to screen for microsatellite markers (ssr) closely linked to rkn resistance in g. hirsutum cv. acala nemx, f1, f2, bc1f1, and f2:7 recombinant inbred lines (rils) from intraspecific crosses and an f2 from an interspecific cross wi ... | 2006 | 16362274 |
| [interaction between structure of plant steroids and their effect on phytonematodes]. | the effects of certain plant steroids of the groups of furostanol glycosides, glycoalkaloids, and alpha-ecdysone on growth and development of phytoparasitic nematodes were studied. it was shown using an experimental system including tomato, lycopersicon esculentum mill., and root-knot nematode, meloidogyne incognita kofoid et white, that steroid molecule had significant nematicidic activity if it contained a carbohydrate moiety and an additional heterocycle in the steroid core. the maximum nemat ... | 2006 | 15029710 |
| synthesis, characterization, and biotoxicity of n n donor sulphonamide imine silicon(iv) complexes. | the organosilicon derivatives of 2-[1-(2-furayl)ethyledene]sulphathiazole with organosilicon chlorides have been synthesised and characterized on the basis of analytical, conductance, and spectroscopic techniques. probable trigonal bipyramidal and octahedral structures for the resulting derivatives have been proposed on the basis of electronic, ir, (1)h, (13)c nmr, and (29)si nmr spectral studies. in the search for better fungicides, bactericides, nematicides, and insecticides studies were condu ... | 2006 | 17496998 |
| root-knot nematode management in double-cropped plasticulture vegetables. | combination treatments of chisel-injected fumigants (methyl bromide, 1,3-d, metam sodium, and chloropicrin) on a first crop, followed by drip-applied fumigants (metam sodium and 1,3-d +/- chloropicrin) on a second crop, with and without oxamyl drip applications were evaluated for control of meloidogyne incognita in three different tests (2002 to 2004) in tifton, ga. first crops were eggplant or tomato, and second crops were cantaloupe, squash, or jalapeno pepper. double-cropped vegetables suffer ... | 2006 | 19259431 |
| atcat6, a sink-tissue-localized transporter for essential amino acids in arabidopsis. | amino acids represent the major form of reduced nitrogen that is transported in plants. amino acid transporters in plants often show tissue-specific expression patterns and are used by plants to transport these metabolites from source to sink during development and under changing environmental conditions. we identified one amino acid transporter, atcat6, which is expressed in sink tissues such as lateral root primordia, flowers and seeds. additionally atcat6 was induced during infestation of roo ... | 2006 | 17052324 |
| soil treatment with hot air (cultivit) as alternative to methyl bromide. | a new development in physical soil treatment is the application of hot air. hot air treatment is based on blowing extremely hot air into rotavating humid soil. the method has been developed and applied commercially in israel for the last few years. an increased growth response (igr) was observed in several crops like potato, cauliflower, kohlrabi and the flower esclepia, when the soil was treated with hot air prior to planting. scientific trials were performed in israel and cyprus to quantify ig ... | 2006 | 17390791 |
| high conservation of the differentially amplified mpa2 satellite dna family in parthenogenetic root-knot nematodes. | sequence variability and distribution of a newly characterized mpa2 satellite dna family are described in five root-knot nematode species of the genus meloidogyne, the mitotic parthenogens m. paranaensis, m. incognita, m. arenaria and m. javanica, and the meiotic/mitotic m. hapla (isolates a and b, respectively). the lack of distinctive mutations and the considerable contribution (40.8%) of ancestral changes disclose an ancient satellite dna which existed in the common ancestor of extant parthen ... | 2006 | 16765538 |
| modulation of reactive oxygen species activities and h2o2 accumulation during compatible and incompatible tomato-root-knot nematode interactions. | here, the interaction of melodoigyne incognita virulent and avirulent pathotypes with susceptible and mi-resistant tomato (solanum lycopersicon) has been studied. significant differences in nematode penetration occurred 2 days postinoculation (dpi) and became stable from 3 dpi onwards. the hypersensitive cell response (hr) in resistant plants prevented the installation of the avirulent pathotype. the virulent pathotype overcame the mi (nematode) resistance and induced feeding sites in root cells ... | 2006 | 16626472 |
| engineering broad root-knot resistance in transgenic plants by rnai silencing of a conserved and essential root-knot nematode parasitism gene. | secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (rkn). parasitism gene 16d10 encodes a conserved rkn secretory peptide that stimulates root growth and functions as a ligand for a putative plant transcription factor. we used in vitro and in vivo rna interference approaches to silence this parasitism gene in rkn and validate that the parasitism gene has an essential function in rkn parasi ... | 2006 | 16985000 |
| evaluation of a nematode bio-product dbx-20% against root-knot nematode meloidogyne incognita affecting grapevine under field conditions. | a field trial was conducted in el-shourouk farm, el-beheira governorate, western nile valley, egypt to determine the effectiveness of the commercial bio-product dbx 1003 20% containing the nematode-trapping fungus dactylaria brochopaga against root-knot nematode meloidogyne incognita infesting grapevine variety superior. its effects on plant growth criteria and yield production were also investigated. the fungus was introduced to soil by either of two ways. first: soil was drenched with spore su ... | 2006 | 17390807 |
| heterologous expression of the mi-1.2 gene from tomato confers resistance against nematodes but not aphids in eggplant. | the mi-1.2 gene in tomato (solanum lycopersicum) is a member of the nucleotide-binding leucine-rich repeat (nblrr) class of plant resistance genes, and confers resistance against root-knot nematodes (meloidogyne spp.), the potato aphid (macrosiphum euphorbiae), and the sweet potato whitefly (bemisia tabaci). mi-1.2 mediates a rapid local defensive response at the site of infection, although the signaling and defensive pathways required for resistance are largely unknown. in this study, eggplant ... | 2006 | 16610741 |
| control of meloidogyne javanica by formulations of inula viscosa leaf extracts. | inula viscosa is a perennial plant that is widely distributed in mediterranean countries. formulations of i. viscosa extracts were tested for their effectiveness in control of meloidogyne javanica in laboratory, growth chamber, microplot, and field experiments. oily pastes were obtained by extraction of dry leaves with a mixture of acetone and n-hexane or n-hexane alone, followed by evaporation of the solvents. emulsifiable concentrate formulations of the pastes killed m. javanica juveniles in s ... | 2006 | 19259429 |
| expression of a plant expansin is involved in the establishment of root knot nematode parasitism in tomato. | a group of plant proteins, expansins, have been identified as wall-loosening factors and as facilitators of cell expansion in vivo. the root knot nematode meloidogyne javanica establishes a permanent feeding site composed of giant cells surrounded by gall tissue. we used quantitative pcr and in situ localization to demonstrate the induction of a tomato (lycopersicon esculentum cv. vf36) expansin (leexpa5) expression in gall cells adjacent to the nematode feeding cells. to further characterize th ... | 2006 | 16395582 |
| effects of the mi-1, n and tabasco genes on infection and reproduction of meloidogyne mayaguensis on tomato and pepper genotypes. | meloidogyne mayaguensis is a damaging root-knot nematode able to reproduce on root-knot nematode-resistant tomato and other economically important crops. in a growth chamber experiment conducted at 22 and 33 degrees c, isolate 1 of m. mayaguensis reproduced at both temperatures on the mi-1-carrying tomato lines bhn 543 and bhn 585, whereas m. incognita race 4 failed to reproduce at 22 degrees c, but reproduced well at 33 degrees c. these results were confirmed in another experiment at 26 +/- 1.8 ... | 2007 | 19259507 |
| low amounts of herbivory by root-knot nematodes affect microbial community dynamics and carbon allocation in the rhizosphere. | increased carbon translocation to the rhizosphere via 'leakage' induced by low amounts of plant parasitic nematodes can foster microorganisms. the effects of the root-knot nematode meloidogyne incognita on microbial biomass (c(mic)) and community structure (phospholipid fatty acids) in the rhizosphere of barley were studied. inoculation densities of 2000, 4000, and 8000 nematodes were well below the threshold level for plant damage. a (13)co(2) pulse-labelling was performed to assess the distrib ... | 2007 | 17916076 |
| management of disease complex caused by root knot nematode and root wilt fungus on pigeonpea through soil organically enriched with vesicular arbuscular mycorrhiza, karanj (pongamia pinnata) oilseed cake and farmyard manure. | this investigation was undertaken to compare the percentage response of colonization and development of va-mycorrhiza (glomus fasciculatum) on a number of pulse crops viz. cowpea, chickpea, soybean, pigeonpea and lentil under glasshouse conditions. among the above-mentioned crops, pigeonpea exhibited the best performance and was selected for further studies. in this host the development and colonization percentage of g. fasciculatum was investigated under two separate substrates i. e. soil amend ... | 2007 | 17978958 |
| root-knot nematode (meloidogyne spp.) me resistance genes in pepper (capsicum annuum l.) are clustered on the p9 chromosome. | the root-knot nematode (meloidogyne spp.) is a major plant pathogen, affecting several solanaceous crops worldwide. in capsicum annuum, resistance to this pathogen is controlled by several independent dominant genes--the me genes. six me genes have previously been shown to be stable at high temperature in three highly resistant and genetically distant accessions: pi 322719, pi 201234, and cm334 (criollo de morelos 334). some genes (me4, mech1, and mech2) are specific to certain meloidogyne speci ... | 2007 | 17136373 |
| cami, a root-knot nematode resistance gene from hot pepper (capsium annuum l.) confers nematode resistance in tomato. | several root-knot nematode (meloidogyne spp.) resistance genes have been discovered in different pepper (capsium annuum l.) lines; however, none of them has yet been cloned. in this study, a candidate root-knot nematode resistance gene (designated as cami) was isolated from the resistant pepper line pr 205 by degenerate pcr amplification combined with the race technique. expression profiling analysis revealed that this gene was highly expressed in roots, leaves, and flowers and expressed at a lo ... | 2007 | 17310335 |
| position of the reduced mycorrhizal colonisation (rmc) locus on the tomato genome map. | our research aims to investigate the molecular communication between land plants and arbuscular mycorrhizal (am) fungi in the establishment of symbiosis. we have identified a mutation in the facultative am host tomato, which we named rmc. plants that are homozygous for rmc no longer host most am fungi. the mutation also affects the interaction of tomato with root knot nematode and fusarium wilt. however, the function/s encoded by the intact rmc locus is/are unknown. to clone and sequence the gen ... | 2007 | 17285306 |
| rnai from plants to nematodes. | coincident with the award of the nobel prize for medicine in 2006 to fire and mello for their discovery of rnai, plant scientists have succeeded in using rnai-based techniques to control nematodes, a hitherto unmanageable plant parasite. recent work has demonstrated that the expression in a host plant of double-stranded rna targeting housekeeping or parasitism genes in the root-knot nematode resulted in resistance to nematode infection. | 2007 | 17254655 |
| molecular cloning and analysis of a new venom allergen-like protein gene from the root-knot nematode meloidogyne incognita. | a new venom allergen-like protein gene isolated from meloidogyne incognita (designated mi-vap-2) was cloned and analysed. the genomic clone of mi-vap-2 is 1917-bp long, contains three introns, which range in size from 39 to 797 bp, and four exons ranging in size from 37 to 361 bp. the cdna of mi-vap-2 contains an open reading frame encoding 294 amino acids, being the first 16 residues a putative secretion signal. southern blot analysis suggested that mi-vp-2 is probably a member of a small multi ... | 2007 | 17481609 |
| ecotypes of the model legume lotus japonicus vary in their interaction phenotypes with the root-knot nematode meloidogyne incognita. | knowledge of host factors affecting plant-nematode interactions is scarce. here, relevant interaction phenotypes between a nodulating model host, lotus japonicus, and the endoparasitic root-knot nematode meloidogyne incognita are assessed via a genetic screen. | 2007 | 17475628 |
| effect of broccoli (brassica oleracea) tissue, incorporated at different depths in a soil column, on meloidogyne incognita. | brassicas have been used frequently for biofumigation, a pest-management strategy based on the release of biocidal volatiles during decomposition of soil-incorporated tissue. however, the role of such volatiles in control of plant-parasitic nematodes is unclear. the goal of this study was to determine the direct localized and indirect volatile effects of amending soil with broccoli tissue on root-knot nematode populations. meloidogyne incognita-infested soil in 50-cm-long tubes was amended with ... | 2007 | 19259479 |
| response of five lettuce cultivars to root-knot nematode, meloidogyne incognita. | the root-knot nematode, meloidogyne incognito (kofoid et white) chitwood is an important pathogen of vegetables. five commercial cultivars of lettuce (lactuca sativa l.) were evaluated under greenhouse conditions for resistance to meloidogyne incognita, benguet population. plants were inoculated with 1000 eggs collected from 'apollo' tomato (lycopersicon esculentum) roots. the degree of galling and number of egg masses were assessed 4 and 8 weeks after inoculation. host plant response was classi ... | 2007 | 18399501 |
| transcriptome analysis of root-knot nematode functions induced in the early stages of parasitism. | root-knot nematodes of the genus meloidogyne are obligate biotrophic parasites able to infest > 2000 plant species. the nematode effectors responsible for disease development are involved in the adaptation of the parasite to its host environment and host response modulation. here, the differences between the transcriptomes of preparasitic exophytic second-stage juveniles (j2) and parasitic endophytic third-stage juveniles (j3) of meloidogyne incognita were investigated. genes up-regulated at the ... | 2007 | 17692078 |
| cloning of the gene lecanicillium psalliotae chitinase lpchi1 and identification of its potential role in the biocontrol of root-knot nematode meloidogyne incognita. | the nematophagous fungus lecanicillium psalliotae (syn. verticillium psalliotae) is a well-known biocontrol agent. in this study, a chitinase gene lpchi1 was isolated for the first time from l. psalliotae using degenerate primers and dna-walking technique. the cloned gene lpchi1 encoding 423 amino acid residues shares a high degree of homology with other pathogenicity-related chitinases from entomopathogenic and mycoparasitic fungi. the complementary dna sequence of the mature chitinase was ampl ... | 2007 | 17665191 |
| nematicidal and larvicidal activities of the essential oils from aerial parts of pectis oligocephala and pectis apodocephala baker. | the chemical composition of the essential oils from aerial parts of pectis apodocephala and pectis oligocephala were analyzed by gc-ms. the essential oils of these species were predominantly constituted by monoterpenes. geranial (42.9-44.5%), neral (32.2-34.2%) and alpha-pinene (10.7-11.4%) were the main constituents in the oil of p. apodocephala, while p-cymene (50.3-70.9%) and thymol (24.4-44.7%), were the prevalent compounds in the oil of p. oligocephala. the essential oils were tested agains ... | 2007 | 17625675 |
| meiotic parthenogenesis in a root-knot nematode results in rapid genomic homozygosity. | many isolates of the plant-parasitic nematode meloidogyne hapla reproduce by facultative meiotic parthenogenesis. sexual crosses can occur, but, in the absence of males, the diploid state appears to be restored by reuniting sister chromosomes of a single meiosis. we have crossed inbred strains of m. hapla that differ in dna markers and produced hybrids and f(2) lines. here we show that heterozygous m. hapla females, upon parthenogenetic reproduction, produce progeny that segregate 1:1 for the pr ... | 2007 | 17483427 |
| comparison of methods for assessing resistance to meloidogyne arenaria in peanut. | use of resistant cultivars is a desirable approach to manage the peanut root-knot nematode (meloidogyne arenaria). to incorporate resistance into commercially acceptable cultivars requires reliable, efficient screening methods. to optimize the resistance screening protocol, a series of greenhouse tests were done using seven genotypes with three levels of resistance to m. arenaria. the three resistance levels could be separated based on gall indices as early as two weeks after inoculation (wai) u ... | 2007 | 19259486 |
| comprehensive transcriptome profiling in tomato reveals a role for glycosyltransferase in mi-mediated nematode resistance. | root-knot nematode (rkn; meloidogyne spp.) is a major crop pathogen worldwide. effective resistance exists for a few plant species, including that conditioned by mi in tomato (solanum lycopersicum). we interrogated the root transcriptome of the resistant (mi+) and susceptible (mi-) cultivars 'motelle' and 'moneymaker,' respectively, during a time-course infection by the mi-susceptible rkn species meloidogyne incognita and the mi-resistant species meloidogyne hapla. in the absence of rkn infectio ... | 2007 | 17434994 |
| the tobacco cel7 gene promoter is auxin-responsive and locally induced in nematode feeding sites of heterologous plants. | summary: emerging evidence suggests that plant cell-wall-modifying enzymes induced by root-parasitic nematodes play important roles in feeding cell formation. we previously identified a tobacco endo-beta-1,4-glucanase (cellulase) gene, ntcel7, that was strongly induced in both root-knot and cyst nematode feeding cells. to characterize further the developmental and nematode-responsive regulation of ntcel7, we isolated the ntcel7 promoter and analysed its expression over a time course of nematode ... | 2007 | 20507511 |
| the mi-9 gene from solanum arcanum conferring heat-stable resistance to root-knot nematodes is a homolog of mi-1. | resistance conferred by the mi-1 gene from solanum peruvianum is effective and widely used for limiting root-knot nematode (meloidogyne spp.) yield loss in tomato (solanum lycopersicum), but the resistance is ineffective at soil temperatures above 28 degrees c. previously, we mapped the heat-stable resistance gene mi-9 in solanum arcanum accession la2157 to the short arm of chromosome 6, in a genetic interval as mi-1 and the cladosporium fulvum resistance gene cf2. we developed a fine map of the ... | 2007 | 17172289 |
| solubilization of insoluble zinc compounds by gluconacetobacter diazotrophicus and the detrimental action of zinc ion (zn2+) and zinc chelates on root knot nematode meloidogyne incognita. | to examine the zinc (zn) solubilization potential and nematicidal properties of gluconacetobacter diazotrophicus. methods and results: atomic absorption spectrophotometer, differential pulse polarography and gas chromatography coupled mass spectrometry were used to estimate the total zn and zn(2+) ions and identify the organic acids present in the culture supernatants. the effect of culture filtrate of zn-amended g. diazotrophicus pal5 on meloidogyne incognita in tomato was examined under gnotob ... | 2007 | 17309498 |
| the mi-1-mediated pest resistance requires hsp90 and sgt1. | the tomato (solanum lycopersicum) mi-1 gene encodes a protein with putative coiled-coil nucleotide-binding site and leucine-rich repeat motifs. mi-1 confers resistance to root-knot nematodes (meloidogyne spp.), potato aphids (macrosiphum euphorbiae), and sweet potato whitefly (bemisia tabaci). to identify genes required in the mi-1-mediated resistance to nematodes and aphids, we used tobacco rattle virus (trv)-based virus-induced gene silencing (vigs) to repress candidate genes and assay for nem ... | 2007 | 17351050 |