Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| echinococcus granulosus: study of the in vitro complement activation by protoscoleces by measuring the electric potential difference across the tegumental membrane. | complement activation by protoscoleces of echinococcus granulosus was studied by analyzing the damage to their tegumental membrane produced by incubation in both normal and hydatid human sera. the state of the apical tegumental membrane was evaluated by measuring the electric potential difference with microelectrodes. protoscoleces incubated in ringer-hepes or in heat-decomplemented normal human serum in the presence or absence of specific antibodies did not show significant variations in the el ... | 1992 | 1426130 |
| evaluation of different immunodiagnostic techniques for diagnosis of hydatidosis in egypt. | in this study detection of the circulating hydatid antibodies was performed by three serological tests: immunoelectrophoresis (iep), enzyme linked immunosorbent assay (elisa) and enzyme linked immunoelectrotransfer blot (eitb) technique. the antigen which was used in these tests was echinococcus granulosus antigen free of host (camel) antigen by immunoaffinity chromatography. most serum samples were pre-selected from the patients on the basis of clinical syndrome or past history of possible diag ... | 1992 | 1431284 |
| genetic variants within the genus echinococcus identified by mitochondrial dna sequencing. | the pattern of species and strain variation within the genus echinococcus is complex and controversial. in an attempt to characterise objectively the various species and strains, the sequence of a region of the mitochondrial cytochrome c oxidase subunit i (co1) gene was determined for 56 echinococcus isolates. eleven different genotypes were detected, including 7 within echinococcus granulosus, and these were used to categorise the isolates. the 4 generally accepted echinococcus species were cle ... | 1992 | 1435857 |
| in vitro culture of the strobilar stage of echinococcus granulosus of sheep and donkey origin from jordan. | protoscolices of echinococcus granulosus isolated from hydatid cysts of sheep and donkeys in jordan were cultured in vitro using a modified diphasic culture system. protoscolices from these two sources manifested differences in the mode of development, evagination and growth rates. protoscolices isolated from sheep cysts grew in vitro in the polyzoic direction up to the three- to four-segmented mature worms, reaching a length of 2.9 mm. in contrast, donkey protoscolices failed to develop beyond ... | 1992 | 1438153 |
| serological evaluation of the 12 kda subunit of antigen b in echinococcus granulosus cyst fluid by immunoblot analysis. | this study evaluated the 12 kda (smallest) subunit of echinococcus granulosus antigen b as a diagnostic molecule. using immunoblotting, 90.9% of cystic hydatid patients, 40% of alveolar hydatid patients and 5.5% of cysticercosis patients showed sero-reactivity to this subunit. human antibody response to the 12 kda molecule appeared independent of factors such as parasite strain or host population responsiveness. the majority of infection sera, and some normal human controls, also recognized the ... | 1992 | 1440787 |
| frequency distribution of echinococcus granulosus hydatid cysts in sheep populations in the xinjiang uygur autonomous region, china. | age-prevalence and age-intensity data of echinococcus granulosus hydatid cysts in sheep populations were collected in an abattoir in the xinjiang uygur autonomous region, people's republic of china. the frequency distribution of the larval cysts per sheep was empirically described by the negative binomial model, with parameter k being 0.5273. a mathematical model for the life cycle of e. granulosus was applied to the collected data and the results show that the infection pressure on sheep was 0. ... | 1992 | 1441193 |
| the efficacy of chemotherapy with mebendazole in human cystic echinococcosis: long-term follow-up of 52 patients. | sixty patients with echinococcus granulosus infection of the liver, lungs, bone and/or soft tissues were treated for several months with oral mebendazole (50-60 mg kg-1 day-1), in divided doses after fat-rich meals, either without surgery (ws), post-surgery (ps) or pre- and post-surgery (pps). long-term follow-up, possible for 52 of the patients, showed that ws, ps and pps patients have so far remained disease-free following treatment for (means +/- standard deviations) 65.5 +/- 37.7, 82.5 +/- 3 ... | 1992 | 1449273 |
| the role of foxes vulpes vulpes in the epidemiology of echinococcus granulosus in urban environments. | to survey the prevalence of intestinal worms, particularly echinococcus granulosus, in foxes in canberra. | 1992 | 1454000 |
| a strategy for production of monoclonal antibodies to echinococcus granulosus antigen 5 and antigen b. | serum antibody responses to sheep hydatid cyst fluid (shcf) and a purified antigen 5 (ag5) were examined in elisa, immunoelectrophoresis (iep) and immunoprecipitation (ip) to facilitate production of monoclonal antibodies (mab) to e. granulosus ag5 and antigen b (agb). although sera from mice immunized with shcf contained antibodies of various classes, the fusions using these donor mice resulted in mainly anti-agb mab, possibly due to the preferential selection of mab to agb by the shcf-based el ... | 1992 | 1459778 |
| echinococcus granulosus in sheep: transfer from ewe to lamb of 'arc 5' antibodies and oncosphere-killing activity, but not protection. | fourteen ewes were orally dosed with 2000 e. granulosus eggs at 2 weeks of age, were mated at 19 months, and produced lambs when the cysts were 2 years old. one week after parturition, all 14 ewes had 'arc 5' antibodies in their serum, as did 11/14 of their lambs. fourteen uninfected ewes were immunized three times before parturition with e. granulosus eggs injected intramuscularly. cysts grew at the first, or first and second site, but not the third, indicating that the ewes were immune prior t ... | 1992 | 1459779 |
| prevalence of human hydatid disease in northwestern libya: a cross-sectional ultrasound study. | a total of 4103 people were screened in an ultrasound survey of the prevalence of hydatid disease (echinococcus granulosus) in five areas of northwestern libya; hydatid cysts were seen in 57 (1.4%), an overall prevalence of approximately 2.0% when adjusted for the likelihood of the occurrence of cysts in other sites in the body. all ultrasound-positive cases were confirmed by dot-blot elisa. the prevalence of hydatid cysts increased with age, and differed between the sexes except in the five to ... | 1992 | 1463358 |
| helminth parasites of carnivores in northern iran. | a total of 100 jackals, 35 stray dogs, 13 cats, two badgers, one fox and one wild cat were collected from two provinces in northern iran. on post mortem examination 41 species of helminths were recovered, at least 12 of which have been reported infecting humans in various countries. hookworms were the most commonly found parasites, and echinococcus granulosus showed the highest intensity of infection. seven helminth species from jackals, three species from dogs, four species from cats and four s ... | 1992 | 1463360 |
| preliminary observations on helminth parasite populations of the dromedary in northern mali. | an abattoir survey on gastrointestinal parasites of the dromedary (camelus dromedarius) was carried out in march/april 1987 in northern mali. parasitological examination of the digestive tract contents of 11 dromedaries revealed that haemonchus longistipes was the predominant parasite with a prevalence as high as 85.7%. the genera impalaia, trichostrongylus, cooperia, and oesophagostomum were also recovered, but with a lower level of infection and frequency. the larval stage of the canine cestod ... | 1992 | 1466143 |
| use of echinococcus granulosus worm antigens for immunodiagnosis of e. granulosus infection in dogs. | echinococcus granulosus worm excretory/secretory antigens (wes) were used in elisa for diagnosis of e. granulosus infection in dogs and compared with protoscolex somatic antigens (psm). sera from 224 dogs were tested. there was no correlation between elisa absorbance values and e. granulosus worm burdens using either antigen. there was a significant linear relationship between absorbance values of sera tested in the elisa using wes (w-elisa) and the elisa using psm (p-elisa). however, there was ... | 1992 | 1485424 |
| limiting dilution analysis of autoreactive t cells in patients affected by hydatid disease. | we evaluated the peripheral autoreactive response in patients with echinococcus granulosus compared with healthy individuals. a limiting dilution analysis (lda) was performed to estimate frequency of auto-reactive t cells and to detect the complex mechanism that often underlies the autoreactive response. different lda curves were seen in healthy controls and patients. the control group provided a straight line passing through the origin ('single hit' kinetics), indicating that only peripheral au ... | 1992 | 1489486 |
| echinococcus granulosus infection of the liver and lung in an 8 1/2-year-old boy. | we report on an 8 1/2-year-old boy with an echinococcus granulosus infection of the liver and lung, drawing particular attention to the therapy applied. this therapy consists on the one hand of medicinal treatment with mebendazol and on the other hand of two subsequent operations with resection of the cysts. the course of the illness is illustrated by means of the most important laboratory parameters; an indication of the present serological diagnostic is also given. | 1992 | 1498102 |
| molecular and immunological diagnosis of echinococcosis. | echinococcosis is an infectious disease of humans caused by the larval (metacestode) stage of the cestode species echinococcus granulosus (cystic echinococcosis or hydatid disease) or echinococcus multilocularis (alveolar echinococcosis or alveolar hydatid disease). clinical manifestations depend primarily on localization and size of hepatic lesions and may include hepatomegaly, obstructive jaundice, or cholangitis. prognostically, alveolar echinococcosis is considered similar to liver malignanc ... | 1992 | 1498767 |
| identification and characterization of specific hydatid antigen fraction(s). | a specific hydatid antigen was prepared in this study from echinococcus granulosus cyst in livers and lungs of camels. elimination of host "camel" protein from crude hydatid fluid was achieved by two methods: salting out using ammonium sulfate precipitation method and immunoaffinity purification using coupled anticamel antibody to cyanogenbromide activated sepharose 4b gel. testing the prepared hydatid antigen against anticamel serum, using immunodiffusion method, indicated that the affinity pur ... | 1992 | 1500792 |
| [echinococcosis in pregnancy]. | hydatid disease affects sheep and cattle and is caused by the larval form of echinococcus granulosus. affected human beings are only chance intermediate hosts. we present a woman admitted in her 22nd week of pregnancy because of upper abdominal pain and persistent vomiting. the presumptive diagnosis of hydatid cyst of the liver was established by ultrasound, x-ray and ct examinations. at laparotomy a 10 x 7 cm hydatid cyst was removed from the liver. she recovered completely and delivered sponta ... | 1992 | 1505822 |
| diagnosis of echinococcus granulosus infection in dogs. | 1992 | 1509663 | |
| [environmental hygienic aspects of echinococcosis]. | a literature review on the current situation of echinococcosis in central europe is given. the only final host for echinococcus granulosus in this region ist the dog. the infection rate of dogs with e. granulosus in central europe is less than 1%. according to meat inspection statistics in germany less than 0.008% of sheep, pigs and horses carry larval stages of e. granulosus. parasitologically confirmed is the occurrence of a cattle-dog strain, the cysts of which were found in 0.26% of slaughte ... | 1992 | 1516550 |
| evaluation of eight serological tests in the diagnosis of human echinococcosis and follow-up. | the elisa with use of igg, iga, and ige, latex agglutination, indirect hemagglutination (iha), total ige, the radioallergosorbent test (rast), and immunoelectrophoresis (iep) were carried out to determine the preoperative diagnosis of infection due to echinococcus granulosus in 131 patients. eighty-nine patients received follow-up care for 42 +/- 22 months (mean +/- sd); 72 were treated surgically and 17 with mebendazole only. we analyzed 5 +/- 2 serum samples per patient and analyzed each serum ... | 1992 | 1520796 |
| the prevalence of echinococcus granulosus in stray dogs in iraq. | the present paper records the prevalence of echinococcus granulosus in stray dogs of al-tamim (northern iraq), diala (mid iraq) and theqar province (southern iraq), where many people are infected with hydatid disease. of 150 dogs examined in three provinces in iraq, 57 (38%) were infected with e. granulosus. the prevalence of the worm was higher in the dogs of theqar province (56%) than in those of al-tamim (20%) and diala provinces (38%). infections were light (1-200 worms) in eight (14%) of th ... | 1992 | 1560486 |
| echinococcus granulosus: possible formation of a shelled egg in vitro. | what appeared to be the early stages in the formation of a single egg with a striated embryophore was observed in an in vitro culture of echinococcus granulosus protoscoleces isolated from sheep hydatid cysts in north jordan. the 'egg' measured 19 x 19 microns in diameter and was formed in an intermediate vesicular/monozoic form which was never previously reported from a culture. this is the first report of an apparently shelled egg forming in an in vitro culture, but although promising, cannot ... | 1992 | 1563913 |
| sylvatic hydatid disease in children: case reports and review of endemic echinococcus granulosus infection in canada and alaska. | 1992 | 1565558 | |
| hydatid disease in a horse. | during routine necropsy of a 28-year-old horse with intestinal volvulus, numerous hydatid cysts were discovered in the liver. viable protoscolices of echinococcus granulosus were obtained from the cyst. as a 4-year-old, this horse had been imported from an area that is enzootic for equine hydatidosis. | 1992 | 1577650 |
| interventional radiology in the liver and pancreas. | the application of interventional radiographic procedures in the management of patients with liver and pancreatic pathology continues to expand. percutaneous hepatobiliary interventional procedures that have received considerable attention in the past year include technical refinements of the transjugular intrahepatic portosystemic shunt procedure used in patients with portal hypertension and esophageal varices, transshunt embolotherapy of persistent varices in patients with small-caliber mesoca ... | 1992 | 1581136 |
| [metastatic echinococcosis as a rare differential diagnosis of therapy refractory bronchitis]. | in a 68 year old male german patient we found a transdiaphragmatic perforation of a superinfected liver echinococcus granulosus cyst into the bronchial system as cause of a purulent bronchitis resistant to treatment. after operative removal and oversewing of diaphragm bronchitis was cured and the patient could be discharged. | 1992 | 1584736 |
| a clinical-radiological evaluation of benzimidazoles in the management of echinococcus granulosus cysts. | nine patients with complicated hydatid disease managed with surgery and mebendazole/albendazole are presented. five patients received albendazole (1 treatment course) and 5 patients received mebendazole (3 had 2 treatment courses, 1 had a switch-over from mebendazole to albendazole). the mean durations of treatment and follow-up were respectively 7 +/- 2.5 months and 7 +/- 2.5 months (albendazole); 13 +/- 10 months and 29 +/- 31 months (mebendazole). a superior clinical and radiological response ... | 1992 | 1589713 |
| evaluation of a monoclonal antibody-based competition elisa for the diagnosis of human hydatidosis. | an antibody competition enzyme-linked immunosorbent assay (elisa) using 4 different monoclonal antibodies (mab) raised against major antigens (antigen 5 and antigen b) of echinococcus granulosus was evaluated for the diagnosis of human hydatidosis. the competition assay, using anti-ag5 mab 24.14, detected specific antibodies in 70% (131/188) of sera from patients with surgically confirmed e. granulosus infection and 38.5% (10/26) of sera from patients with e. multilocularis infection. none of th ... | 1992 | 1594299 |
| immunodiagnosis of human cysticercosis (taenia solium): a field comparison of an antibody-enzyme-linked immunosorbent assay (elisa), an antigen-elisa, and an enzyme-linked immunoelectrotransfer blot (eitb) assay in peru. the cysticercosis working group in peru (cwg). | we compared results of an enzyme-linked immunosorbent assay (elisa) and an enzyme-linked immunoelectrotransfer blot (eitb) assay for the diagnosis of cysticercosis in sera and cerebrospinal fluid (csf). sera from 34 patients with confirmed cysticercosis were tested by both elisa and eitb assays. cerebrospinal fluid from some of these patients was also tested by elisa for the presence of antibody (ab-elisa) (n = 21) and antigen (ag-elisa) (n = 15). specificity in sera was examined by testing 51 s ... | 1992 | 1599056 |
| [bone hydatidosis. 12 cases]. | the hydatic cyst is a parasitic disease caused by the development in man of the larval form of echinococcus granulosus. all parts of the body may be involved, but the liver and lungs are the main locations of the disease. the bone site is rare, accounting for 0.9 to 2% of all hydatic lesions. between 1982 and 1988, we have compiled 12 cases of hydatic disease of bone. they were located in the skull (4 cases), the iliac bone (4 cases), the omoplate (1 case), the ribs (1 case), the femur (1 case) ... | 1992 | 1599580 |
| immunological localization of blood pl antigen in tissues of echinococcus granulosus. | 1992 | 1616399 | |
| enzyme-linked immunoelectrotransfer blot test for diagnosis of human hydatid disease. | sera from 71 patients with surgically confirmed hydatid disease (which is caused by echinococcus granulosus) were studied by an enzyme-linked immunoelectrotransfer blot (eitb) assay. sera from patients either with other cestode infections or with another illness were used as controls. results of the eitb test for hydatidosis were compared with those of the double-diffusion (dd5) test and an enzyme-linked immunosorbent assay (elisa). in the eitb assay with bovine lyophilized hydatid fluid, three ... | 1992 | 1624574 |
| examination of murine antibody response to secondary hydatidosis using elisa and immunoelectrophoresis. | antibody responses in mice with up to 64 weeks of secondary echinococcus granulosus hydatidosis were examined by elisa using hydatid protoscolex antigen (px), antigen 5 (ag5) and antigen b (agb), and by immunoelectrophoresis (iep) using sheep hydatid cyst fluid (shcf). anti-px igg antibodies, evident from 3-5 days post infection (p.i.), increased steadily until 16 weeks and maintained a high level afterwards. anti-ag5 igg antibodies were negligible up to two weeks, but they showed a small increa ... | 1992 | 1625903 |
| cyclophilin of schistosoma japonicum. | a 623-bp cdna molecule encoding cyclophilin, a specific cyclosporin a-binding protein, has been isolated from schistosoma japonicum using a heterologous cdna probe from echinococcus granulosus. the nucleotide sequence of this molecule has been determined, and the deduced amino acid sequence has revealed extensive homology with homologues of other species. southern blot analysis suggests that s. japonicum cyclophilin is the product of a single-copy gene. the cloning of this cdna will allow an inv ... | 1992 | 1635025 |
| viability of echinococcus granulosus cysts in mice following cultivation in vitro. | the viability of hydatid cysts developed in vitro for 90 days was assessed by implantation into mice. cysts removed from mice at 270 days post-infection (p.i.) increased their size 13.5-fold and contained several brood capsules containing protoscoleces. thus, cysts remain viable after prolonged in vitro culture. the implantation in mice of 15 cysts developed in vitro yielded an average of 10 cysts per mouse, which is indicative of a high survival rate in these experimental infections. the ultras ... | 1992 | 1639569 |
| comparative cytotoxicity of secondary hydatid cysts, protoscoleces, and in vitro developed microcysts of echinococcus granulosus. | infection with the metacestode of echinococcus granulosus is characterized by a concomitant immunity. survival of established and developing hydatid cysts in the intermediate host implies a mechanism to modulate its immunological reactions. in order to investigate this mechanism, secondary hydatid cysts were isolated from intraperitoneally infected laboratory white mice (strain nmri) 12 months p.i. a number of hydatid cysts were freed from the surrounding host adventitial tissue. monolayer cultu ... | 1992 | 1640086 |
| echinococcus granulosus: use of an intermediate host mouse model to evaluate sources of protective antigens and a role for antibody in the immune response. | a balb/cj mouse model was used to determine which stage of the e. granulosus life cycle possessed the most potent protective antigens. mice were immunized with crude extracts of protoscoleces, brood capsules, cyst fluid, adult worm tissue, eggs or oncospheres and then challenged intraperitoneally with 600 activated oncospheres. sonically disrupted oncospheres induced the highest levels of protection (greater than 90%) at doses greater than or equal to 10(3) oncosphere equivalents per mouse. high ... | 1992 | 1644518 |
| a case of natural hydatidosis in a baboon. | four large and several smaller cysts of echinococcus granulosus were found in the liver of a baboon submitted for necropsy. three of the large cysts had an atypical structure and were packed with a semi-solid mass of collapsed daughter cysts, small cysts containing fluid and involuted membranes. this atypical structure has been reported previously though only in baboons. | 1992 | 1644938 |
| diagnostic value of a synthetic peptide derived from echinococcus granulosus recombinant protein. | a specific monoclonal antibody (mab; eg 02 154/12) directed against a protein epitope of echinococcus granulosus antigen 5 was used to screen a cdna library constructed from e. granulosus protoscoleces rna. one clone designated eg14 was selected and shown to code for an amino acid sequence partially homologous to that of the clone eg6 previously identified with the same mab. hydrophobic cluster analysis showed that both recombinant antigens may adopt a similar alpha-helical organization and shar ... | 1992 | 1737836 |
| albendazole in hydatid disease: results in 22 patients. | a prospective study to evaluate the effect of albendazole in the treatment of echinococcus granulosus was conducted at the gastroenterology unit, armed forces hospital, riyadh since april 1985 to present involving 22 patients mainly with liver hydatid disease. of these patients, 30% had a previous history of surgery for recurrence of previously operated hydatid cysts. the duration treatment and follow up period ranged from two months to three years. the only side effect was hair loss in two pati ... | 1992 | 17589144 |
| analysis of taeniid antigens using monoclonal antibodies to echinococcus granulosus antigen 5 and antigen b. | antigens derived from echinococcus granulosus, taenia hydatigena and t. pisiformis cyst fluids, t. solium cysticerci, e. multilocularis protoscoleces and e. vogeli cyst membranes were examined by enzyme-linked immunosorbent assay (elisa) and immunoelectrophoresis (iep) using four monoclonal antibodies (mab) to e. granulosus antigen 5 (ag5) and antigen b (agb). anti-ag5 mabs 24.14 and 61a12 reacted strongly with t. hydatigena and t. pisiformis cyst fluids and, to a lesser degree, anti-agb mabs 31 ... | 1993 | 7682330 |
| echinococcus granulosus:isoprinosine treatment of the metacestode stage. | the efficacy of short- and long-term treatments with isoprinosine, an immunomodulatory compound, was studied in echinococcus granulosus cysts developed in nmri mice intraperitoneally infected with sheep pulmonary cysts. after treatment, a reduction in the size and number of cysts with macroscopic modifications was observed. the structural alterations included damage or destruction of the protoscoleces and partial destruction of the cyst wall, which predominated at the inner germinal layer level. ... | 1993 | 7685983 |
| chromatographic and antigenic properties of echinococcus granulosus hydatid cyst-derived glycolipids. | the neutral and acidic fraction glycolipids of echinococcus granulosus metacestode tissue compartments were isolated, defined by their chromatographic and antigenic properties, and assessed as to their efficacy as antigens in the serodiagnosis of human hepatic cystic and alveolar echinococcosis, and other helminthiases. analyses were accomplished by thin-layer chromatography immunostaining and elisa. the neutral glycolipid fraction's major carbohydrate epitope was the same as or very similar to ... | 1993 | 7533281 |
| [rflp analysis of dna from echinococcus granulosus collected from four provinces/autonomous region in china]. | a fragment of the ribosomal rna gene of schistosoma mansoni psm889 and two dna fragments specific to echinococcus granulosus phd5 and peg18 have been used as dna probes to assess the extent of genetic variability within e. granulosus. the dna analysis, including restriction endonuclease digestion and southern blot hybridization with the probes, did not demonstrate any genetic variation among e. granulosus collected from sheep in xinjiang, qinghai, gansu and ninxia. similarly, there was no geneti ... | 1993 | 7909504 |
| [pulmonary echinococcosis, hepatic opisthorchiasis and generalized trichinelliasis combined with pregnancy]. | a case is reported of a spontaneous abortion (25 weeks of gestation) in a nenets woman resulting in thrombohemorrhagic syndrome and profuse uterine bleeding. the abortion is attributed to drastic allergization by three parasites: echinococcus granulosus, opisthorchis felineus, trichinella spiralis. | 1993 | 7980070 |
| intestinal helminth parasite community in wolves (canis lupus) in italy. | from 1987 to 1993, 89 wolves (canis lupus) collected throughout the whole italian range were examined for intestinal helminth parasites. twelve species were found, including 5 nematodes (uncinaria stenocephala, toxocara canis, ancylostoma caninum, trichuris vulpis and toxascaris leonina) and 7 cestodes (echinococcus granulosus, taenia hydatigena, t. multiceps, t. pisiformis, t. ovis, mesocestoides lineatus and dipylidium caninum). no significant differences were detected between sexes. t. canis ... | 1993 | 8065823 |
| rapid discrimination of echinococcus species and strains using a polymerase chain reaction-based rflp method. | echinococcus species and genetically distinct strains of echinococcus granulosus can be rapidly and reliably identified using a polymerase chain reaction (pcr)-linked restriction fragment length polymorphism (rflp) method which surveys the sequence of a rapidly evolving region of the ribosomal dna (rdna) unit. internal transcribed spacer 1 (its1) of the rdna repeat was amplified from various isolates and the product was digested with one of a number of 4-base cutting restriction enzymes. charact ... | 1993 | 8094539 |
| molecular examination of the sympatry and distribution of sheep and camel strains of echinococcus granulosus in kenya. | previous studies have shown that camel and sheep strains of the cystic hydatid parasite echinococcus granulosus occur in kenya. we examined 208 larval isolates and 40 worm samples of e. granulosus from various hosts in kenya using restriction fragment length polymorphism analysis of a segment of ribosomal dna amplified by the polymerase chain reaction. this was in an effort to determine whether additional strains of e. granulosus occur in kenya, to examine the level of genetic heterogeneity with ... | 1993 | 8097619 |
| host influence on the rate of maturation of echinococcus granulosus in dogs in kenya. | 1993 | 8122923 | |
| high performance latex reagent for hydatid serology using an echinococcus granulosus lipoprotein antigen fraction purified from cyst fluid in one step. | a lipoprotein fraction from fertile bovine hydatid cyst fluid (fbhcf) was isolated by affinity chromatography on acrylic-heparin. the heparin-binding lipoprotein fraction (hblf) was proved to be free from host immunoglobulins and albumin. immunoblotting with confirmed human hydatid sera showed that the major relevant diagnostic bands in fbhcf were also present in hblf. hblf or fbhcf were used to sensitize polystyrene latex particles. hblf-latex showed both higher reactivity with positive hydatid ... | 1993 | 8225758 |
| epidemiology of hydatidosis and echinococcosis in theqar province, southern iraq. | ninety-six cases of human hydatidosis caused by echinococcus granulosus were diagnosed from surgical records of hospitals in theqar province, southern iraq during 1989. the infection rate was higher in female than in male patients (56 versus 40 cases) and increased with their age (maximum, 25.0% in a female group of 31-40 years and 32.5% in a male group of 41-50 years). many organs such as liver, lungs, peritoneum and spleen were involved, but the liver was most frequently affected. of 96 patien ... | 1993 | 8230806 |
| molecular cloning and characterization of actin genes from echinococcus granulosus. | an echinococcus granulosus genomic library has been screened with a mouse beta-actin cdna probe. two clones carrying dna fragments of about 15 kb, possibly derived from the same genome region, have been isolated. this 15-kb genomic region includes 2 actin-related sequences (egacti and egactii) separated by about 4 kb. the nucleotide sequences of both genes were determined. the egacti sequence presents no introns, but an intron of 591 bp was observed in the egactii sequence. the genes potentially ... | 1993 | 8232413 |
| ultrastructural localization of major hydatid fluid antigens in brood capsules and protoscoleces of echinococcus granulosus of human origin. | monospecific rabbit antisera obtained through experimental immunization with previously purified proteins were used in the ultrastructural localization of two hydatid fluid antigens, in brood capsules and protoscoleces of echinococcus granulosus of human origin. the antigen-antibody reaction was revealed by a colloidal gold based method. reaction was evident in the connective region of the germinal membrane and in the parenchyma of the protoscoleces. both antigen 5 and antigen b were located in ... | 1993 | 8233559 |
| igg subclasses in human hydatid disease: prominence of the igg4 response. | to assess the participation of the four subclasses of igg in the humoral response to echinococcus granulosus infection, we determined total and parasite-specific igg1, igg2, igg3 and igg4 in sera from 46 patients with hydatid disease using an enzyme-linked immunosorbent assay (elisa). parasite-specific igg subclass antibodies were quantitatively measured by means of standard curves obtained by affinity chromatography. sera from 35 healthy individuals served as controls. the total component of ig ... | 1993 | 8241797 |
| dose-dependent effects of hydatid fluid toxins from echinococcus granulosus on mouse peritoneal macrophages. | hydatid fluid (hf) from hydatid cysts of the cestode echinococcus granulosus inhibited the phagocytosis of bacteria and yeast cells by host macrophages in vitro. different assays were used to study the dose-dependent effect of partially purified hf toxins on peritoneal macrophages (pm phi) of the mouse. trypan blue exclusion, as well as measuring the specific release of lactate dehydrogenase activity and 51cr release of 51cr-labelled pm phi, showed that incubation with hf toxins lead to the lysi ... | 1993 | 8244205 |
| echinococcal disease. | diagnosis of infection by the larval stages of echinococcus granulosus, e. multilocaris, and e. vogeli, has increased in most parts of the world because of improved diagnostic technology, active surveillance, and increasing rates of transmission. specific immunodiagnostics and sophisticated imaging techniques have made diagnosis more sensitive and specific. surgery, performed by an experienced team with adequate postoperative support, remains the mainstay of therapy; however, alternative treatme ... | 1993 | 8254162 |
| albendazole therapy of hydatid disease: 2-year follow-up of 40 cases. | forty patients with 63 echinococcus granulosus cysts affecting different sites were treated with albendazole and have been followed up for at least 24 months from completion of therapy. twenty-one patients (53%) with 37 cysts (59%) showed evidence of healing. the criteria and pattern of healing are outlined. the most serious complication of albendazole therapy was hepatoxic jaundice, which occurred in 5% of patients. recurrence during the observation period was encountered in 9.5% of patients wi ... | 1993 | 8257234 |
| giant hydatid lung cysts in the canadian northwest: outcome of conservative treatment in three children. | hydatid lung disease due to echinococcus granulosus in the canadian northwest and alaska is often asymptomatic and usually benign. we reviewed the course and outcome of three children with giant hydatid lung cyst seen over a 2-year period. all were north american indian children aged 9 to 12 years who presented with cough, fever, and chest pain. one had a rash. there was a history of exposure to domestic dogs who had been fed moose entrails in each case. chest x-rays showed solitary lung cysts w ... | 1993 | 8308679 |
| random amplified polymorphic dna technique for speciation studies of echinococcus granulosus. | 1993 | 8327458 | |
| heart transplantation in a patient with liver hydatidosis. | the human can serve as an accidental intermediate host to echinococcus granulosus, a parasite that targets dogs as its definitive hosts. we present the case of a 27-year-old man with liver hydatidosis, who underwent heart transplantation because of ischemic cardiomyopathy. the immunosuppressor treatment had no effect on the size of the cysts, which were removed surgically 14 months after heart transplantation; good results persist after 3 years of follow-up. | 1993 | 8329434 |
| isolation and characterization of a middle repetitive dna element from echinococcus granulosus. | 1993 | 8341331 | |
| the potential of vervet monkeys (cercopithecus aethiops) and baboons (papio anubis) as models for the study of the immunology of echinococcus granulosus infections. | nine vervet monkeys and nine baboons were infected with eggs of echinococcus granulosus per os. six of the vervets and one of the baboons possessed hydatid cysts at autopsy, 15-28 months post-infection. the sequential igg response to hydatid fluid and protoscolex antigens showed considerable inter-animal variation. infected vervets and baboons became seropositive after an average of 8 months post-infection. considerable fluctuation in the igg response was observed, particularly to the hydatid fl ... | 1993 | 8341587 |
| [surgical management of liver echinococcosis]. | in the last ten years 62 patients were operated on by the authors because of liver echinococcosis. each of the infections caused by echinococcus granulosus. in the course of the examination ultrasonography, abdominal x-ray, casoni test and elisa were used. angiography and ercp were applied only to answer special questions. the echinococcus cysts were removed in 15 cases by atypical liver resection, in 42 cases by pericystectomy and in 5 cases by partial cystectomy using the pringle's manoeuvre i ... | 1993 | 8351130 |
| effects of mebendazole, albendazole, and praziquantel on succinate dehydrogenase, fumarate reductase, and malate dehydrogenase in echinococcus granulosus cysts harbored in mice. | echinococcus granulosus cyst wall possess high biochemical activities of malate dehydrogenase (md) and fumarate reductase (fr), but low activity of succinate dehydrogenase (sd), suggesting that the cyst wall may utilize a partial reverse tricarboxylic acid cycle. when infected mice were given intragastrically with mebendazole, 25-50 mg.kg-1.d-1, albendazole 300 mg.kg-1.d-1 or praziquantel 500 mg.kg-1.d-1 for 7-14 d, no apparent effects on sd and fr activities of the cyst wall were found, while t ... | 1993 | 8352009 |
| distribution of some elements in hydatid cysts of echinococcus granulosus from buffalo (bubalus bubalis). | distribution of some elements during development of endogenous daughter (hydatid) cysts of echinococcus granulosus of buffalo origin was determined by atomic absorption spectrophotometry. these analyses showed that the distribution of copper and cobalt was highest in the smallest cysts. these elements gradually decreased in the cysts as they enlarged. the distribution of zinc, iron and manganese was very high in the smallest cysts in comparison to copper and cobalt, but these three elements decr ... | 1993 | 8354855 |
| sudden death due to ruptured hydatid cyst of the liver. | the cyst stage of echinococcus granulosus is commonly located in the liver, which frequently results in a long symptomless period. we present a sudden death due to a freshly hemorrhagic hydatid cyst following blunt trauma to the liver in a 19-year-old boy. the possible mechanism was thought to be an anaphylactoid reaction to the leakage of the cyst fluid. | 1993 | 8355014 |
| anthelmintic effects of cyclosporin a on protoscoleces and secondary hydatid cysts of echinococcus granulosus in the mouse. | cyclosporin a (csa), employed primarily as an immunosuppressant during the management of organ and graft transplants, exhibits anthelmintic properties. however, its efficacy against tapeworm infections in laboratory models is variable. a preliminary investigation has been undertaken to assess the action of csa on the establishment and growth of protoscoleces and secondary hydatid cysts of ovine echinococcus granulosus in mice. administration of csa in five consecutive daily doses, beginning 2 da ... | 1993 | 8359980 |
| [echinococcosis--current status of diagnosis and therapy]. | the most frequent parasitosis in europe is the infection by echinococcus spp. echinococcus granulosus is identified worldwide, 60% of all human infection are found in the liver, where cysts (hydatides) are built. echinococcus alveolaris is found in the northern hemisphere, endemic in middle europe in southern germany, austria and parts of switzerland. 98% of all human infection are localized in the liver, where infiltrative growth destroying the tissue is found. diagnosis is based on serological ... | 1993 | 8366752 |
| molecular cloning and characterization of an echinococcus granulosus cdna encoding malate dehydrogenase. | 1993 | 8366891 | |
| intracranial hydatid cysts in children. | brain involvement in hydatid disease occurs in 1 to 2% of all echinococcus granulosus infections. fifty to 75% of intracranial hydatid cysts are seen in children. this study included 19 children who underwent surgery for intracranial hydatid cysts between january 1979 and september 1992. there were 12 boys and 7 girls, ages 3 to 16 years (mean, 8.1 yr). headache and vomiting were the predominant symptoms. papilledema was present in 16 patients, and 2 patients had secondary optic atrophy. a round ... | 1993 | 8367043 |
| [hepatic alveolar hydatidosis]. | alveolar echinococcosis of the liver is a disease caused by the intrahepatic growth of echinococcus multilocularis larvae. in contrast to hydatidic cyst caused by echinococcus granulosus, this is a rare disease in spain. we present a case of alveolar echinococcosis of the liver which presented with jaundice and hepatomegaly. an infiltrative solid tumor was diagnosed by us and ct scan. the patient required left hepatectomy and interventional radiology procedures with satisfactory results. diagnos ... | 1993 | 8398373 |
| [screening antihydatid drugs using cultivated germinal cells of echinococcus granulosus]. | germinal cells isolated from echinococcus granulosus cysts harbored in mice have been maintained in an in vitro culture system containing rpmi 1640 supplemented by 20% calf serum, and used as a model for screening anti-hydatid drugs. when the germinal cells were maintained in the medium for 6 days, the cell proliferation rate was rather high in the first four days but declined in the last two days. in screening drugs, 1.4 x 10(6) germinal cells were exposed to known effective drugs against metac ... | 1993 | 8403269 |
| [elib of specific antigens in in vitro cultivated germinal cells of echinococcus granulosus]. | specific antigens are detected in cyst fluid and cyst wall of echinococcus granulosus, as well as germinal cells cultivated in vitro by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) and enzyme linked immunotransfer blot technique (elib), using the sera from mice infected with protoscoleces of e. granulosus for at least 10 months. a specific reaction band of 52 kda or 38 kda was detected in soluble protein of germinal cells and cyst fluid, respectively, but these two react ... | 1993 | 8403282 |
| [preliminary study on monoclonal antibodies against cysticercus cellulosae]. | in order to produce valuable monoclonal antibodies for immunodiagnosis of cysticercosis, hybridoma technique was adopted using the fusion of splenic lymphocytes from balb/c mice immunized with cysticercus cellulosae antigens and the myeloma cell line ns-1. through screening and cloning, three monoclonal antibody cell lines have been established. immunoglobulin subclasses of 3 lines were determined to be igg1. these monoclonal antibodies were found to be highly specific and without cross reaction ... | 1993 | 8403283 |
| improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the em2plus antigen. | alveolar echinococcosis (ae) in humans is generally a fatal disease when not diagnosed early enough to provide curative treatment such as radical surgery. immunodiagnosis for early detection of ae was improved by the isolation of an affinity-purified metacestode em2 antigen and by the synthesis of recombinant echinococcus multilocularis antigen ii/3-10. both antigens were individually assessed by enzyme-linked immunosorbent assay (elisa) and demonstrated high specificities and diagnostic sensiti ... | 1993 | 8432825 |
| morphological characterization of adult echinococcus granulosus as a means of determining transmission patterns. | host induced changes in morphological characters of the rostellar hooks of echinococcus granulosus were used to determine the origin of infection in definitive hosts in rural areas of southeastern australia where wild and domestic cycles of transmission may interact. the morphological characters studied vary depending on the species of intermediate host (macropod marsupials, sheep) in which protoscoleces develop, and these characters are retained in adult worms. it was therefore possible to dete ... | 1993 | 8437058 |
| partial characterization of antigens in circulating immune complexes in cystic hydatid patients treated with albendazole. | immune complexes were precipitated by 3% polyethylene glycol (peg) from 4 sera from albendazole-treated hydatid patients from uruguay, and together with sheep hydatid cyst fluid were analysed by ion-exchange fast protein liquid chromatography (fplc). fplc-eluted fractions of serum preparations and cyst fluid were tested in an enzyme-linked immunosorbent assay for the detection of specific echinococcus granulosus antigens and also for presence of specific immunoglobulin (ig) m and igg in the seru ... | 1993 | 8465413 |
| echinococcus granulosus hydatid cysts in the livers of two horses. | 1993 | 8466972 | |
| further evidence for the occurrence of a distinct strain of echinococcus granulosus in european pigs. | the morphology, adult development and genetic characteristics of echinococcus granulosus isolated from pigs in poland were examined and compared with those of other recognised strains of e. granulosus. the isolates were characterised by their distinct morphology, rapid maturation and unique dna hybridisation profiles. the form of e. granulosus that occurs in european pigs may therefore be a distinct strain that can be separated morphologically and genetically from other strains and that exhibits ... | 1993 | 8469670 |
| a developmentally regulated gene of echinococcus granulosus codes for a 15.5-kilodalton polypeptide related to fatty acid binding proteins. | a stage-specific expressed gene has been isolated from a cdna expression library of echinococcus granulosus protoscolices. the isolated clone contains the complete coding sequence. the corresponding protein (egdf1) has a molecular weight of 15.5 kda and is expressed at the tegumental level in the protoscolices, being undetectable in the germinal layer of the metacestode. this protein shares an important homology with a family of low-molecular weight proteins involved in the binding of hydrophobi ... | 1993 | 8479446 |
| molecular cloning of an echinococcal microtrichal antigen immunoreactive in echinococcus multilocularis disease. | a cdna expression library of the larval stage of the cestode worm echinococcus multilocularis has been established in the phage lambda zapii system. by immunoscreening with pooled sera from patients with alveolar echinococcosis an immunoreactive clone, termed pem13, was isolated. em13 was expressed using the expression vector pgex-3x, resulting in the synthesis of a glutathione s-transferase fusion protein. 82% of the sera from 28 patients suffering from e. multilocularis disease had antibodies ... | 1993 | 8479454 |
| assessment of monoclonal antibodies to echinococcus granulosus antigen 5 and antigen b for detection of human hydatid circulating antigens. | four monoclonal antibodies (mab) to echinococcus granulosus antigen 5 (ag5) and antigen b (agb) were assessed in an enzyme-linked immunosorbent assay (elisa) for detection of circulating antigens (cag) in sera of human patients with e. granulosus infection. around 5.5-8% of 200 sera from 42 surgically proven hydatid patients contained detectable cag by individual mab. the combined detection rate for cag, using four mab, was 19% (38/200). although hydatid cag was detected by mab in at least one s ... | 1993 | 8479805 |
| ultrastructure and microanalyses of the calcareous corpuscles of the protoscoleces of echinococcus granulosus. | the calcareous corpuscles of the protoscolex stage of echinococcus granulosus are irregularly spherical or ovoid in shape and have a diameter ranging between 2 and 16 microns. the central region of immature corpuscles is composed of an electron-lucent matrix containing granular deposits and, in more mature corpuscles, paired membrane lamellae. energy-dispersive x-ray microanalysis of sectioned immature corpuscles demonstrated calcium, magnesium and phosphorus, whilst a quantitative analysis indi ... | 1993 | 8493249 |
| survey of echinococcosis in eastern ethiopia. | a total of 171 adult cattle and nine stray dogs were examined for echinococcus granulosus in eastern ethiopia. prevalences of 20.5% and 22% were recorded in cattle and dogs, respectively. some of the factors involved in the transmission dynamics are discussed. | 1993 | 8493763 |
| [effects of mebendazole on glucose, glycogen, lactic acid, and lactate dehydrogenase in echinococcus granulosus cyst wall]. | the glucose, glycogen, and lactic acid contents in echinococcus granulosus cyst wall were 3570 +/- 623, 1276 +/- 126, and 267 +/- 29 micrograms.g-1, respectively. the lactate dehydrogenase (ldh) activity in the cyst wall was 4307 +/- 297 u/(min.mg protein). when infected mice were treated ig with mebendazole (meb) 25 or 50 mg.kg-1.d-1 for 7-14 d, the glucose and glycogen contents in the cyst wall decreased markedly. no apparent changes in lactic acid and ldh activity were seen. the results sugge ... | 1993 | 8503286 |
| isolation & immunochemical characterization of diagnostically relevant antigens of echinococcus granulosus. | two hydatid specific polypeptides with molecular masses of 8 kda and 116 kda have been successfully isolated from e. granulosus hydatid cyst fluid using affinity chromatography. sodium dodecyl sulphate polyacrylamide gel electrophoresis and western immunoblot analysis under reducing and denaturing conditions indicated the 116 kda purified antigen to be a hetero-tetramer consisting of 45 kda, 66 kda, 75 kda and 116 kda subunits linked by disulphide bonds while the 8 kda purified antigen was found ... | 1993 | 8505079 |
| treatment of echinococcus granulosus cysts. | 1993 | 8511523 | |
| [human echinococcosis: the role of histocompatibility antigens in realizing infestations and the characteristics of their course]. | the role of histocompatibility antigens in the realization of invasions and the nature of its course were studied in 71 patients with echinococcus granulosus infection and in 18 with e. multilocularis infection. it has been shown that the effects of hla antigens on the realization of invasion is heterodirectional; the factors predisposing to the occurrence of echinococcosis are hla-b5, b18, and a28 antigens, whereas those preventing their realization are hla-b14 antigen. the carriage of hla-b5 a ... | 1993 | 8127263 |
| [characterization of echinococcus granulosus and echinococcus multilocularis by dna probe]. | cloned dna fragments phd5, psm889 and peg18 have been used as dna probes in the restriction endonuclease analysis and southern blot hybridization to characterize e. granulosus and e. multilocularis protoscolices from china. southern blot hybridization method is sensitive, specific and has the advantage in identification over microscopic examination. the authors deem that it can be used in the base-line epidemiological survey and surveillance of hydatid disease to provide data for hydatid disease ... | 1993 | 8168243 |
| studies on echinococcus granulosus using the scanning electron microscope. i. preparations of the parasite for infection of the final host. | the development of the protoscoleces of echinococcus granulosus was studied after chemical stimulation, which mimics that offered by the final host. this stimulation resulted in some morphological changes in the parasite and to its evagination. these changes prepare the parasite to infect the intestine of its final host. the external morphology of the protoscolex body and of the germinal layer of the cyst and brood capsule were studied using the scanning electron microscope (sem). findings were ... | 1993 | 8278334 |
| studies on echinococcus granulosus using the scanning electron microscope. ii. the hooks. | the morphology of the hooks of echinococcus granulosus at the protoscolex level was studied by means of scanning electron microscopy. hooks were uncovered in situ and also freed from the rostellum so as to study them in detail. there were two types of hooks: those of the upper row, which were larger and had a projecting, rounded stout guard, and those of the lower row, which were smaller and had a flattened guard. these and other differences in the morphology of the two types of hooks were studi ... | 1993 | 8278335 |
| [hydatidosis immunodiagnosis: determination of taenia crassiceps cysticerws antigens in hydatid and vesicular fluid]. | the specificity and sensitivity of the enzyme immunoassay (eia), presently used in south america areas where hydatidosis caused by echinococcus granulosus is endemic, was compared to two alternative eia. one of these uses an hydatid antigen of different preparation and the other vesicular fluid of taenia crassiceps cisticerci (vfcc). the effect of previous neutralization in the serum sample of antibodies antinormal ovine or murine sera and antiphosphorylcholine on the diagnostic efficiency of th ... | 1993 | 8284600 |
| some observations on hydatidosis in jordan. | a total of 2.8% (5 of 176) sera from schoolchildren and university students were seropositive for hydatidosis (echinococcus granulosus), by the elisa ige test. the prevalence of seropositivity was 4.5% among females of all age groups (5-24 years) and among males of the age group 18-24 years. males of the age group (5-17 years) were seronegative. the ratio of seropositivity between schoolboys and schoolgirls was 1:4. sera collected from 1085 university students, aged 18-24 years, and from 1656 ou ... | 1993 | 8288858 |
| paramyosin of echinococcus granulosus: cdna sequence and characterization of a tegumental antigen. | a lambda zapii cdna library of echinococcus granulosus larvae was expressed in escherichia coli sure cells. screening of the library with a rabbit antiserum raised against total larval antigen yielded several immunoreactive clones. for analysis of the nucleotide sequence, in vivo excision into pblueskript was carried out and the 3' end of the cloned insert was sequenced. three of these clones exhibited identical nucleotide sequences, suggesting expression of identical genes. the complete nucleot ... | 1993 | 8295903 |
| intestinal helminths of public health importance in dogs in nairobi. | in a survey of intestinal canine zoonotic helminths in nairobi, 156 dogs were autopsied. of these, 10% were infected with echinococcus granulosus, 88% with ancylostoma caninum, 45% with dipylidium caninum and 3% with toxocara canis. all the infected dogs with e. granulosus originated from around abottoirs in dagoretti. the risk of infection in man with hydatid disease is considered to be high around dagoretti and control measures are recommended. | 1993 | 8187655 |
| serological differentiation between cystic and alveolar echinococcosis by use of recombinant larval antigens. | two recombinant antigens of the larval stages of echinococcus granulosus and echinococcus multilocularis, termed eg55 and em10, respectively, were applied for serodiagnosis and serological differentiation between parasitic infections caused by the metacestode tissue of both tapeworms. antigen em10 is synthesized by e. multilocularis larvae. antigen eg55 represents the recombinant form of the low-molecular-weight subunit of antigen b, which is an echinococcus genus-specific antigen. both recombin ... | 1993 | 8308113 |
| echinococcus granulosus (taeniidae) and autochthonous echinococcosis in a north american horse. | we report the first documented case of autochthonous echinococcosis in a horse of north american origin. three fully mature and viable unilocular hydatid cysts of echinococcus granulosus (batsch, 1786) were an incidental finding at necropsy in the liver of a 14-yr-old gelding thoroughbred that had been foaled in virginia and raised in maryland. protoscolices were armed with 2 rows of 28-37 rostellar hooks; small hooks measured 23-30 microns; large hooks measured 26-33 microns. morphologically, t ... | 1994 | 8308647 |
| us case of the day. hydatid cyst (echinococcus granulosus) in the right lobe of the liver. | 1994 | 8190968 |