Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| opisthorchiasis and cholangiocarcinoma in southeast asia: an unresolved problem. | the prevalence of cholangiocarcinoma (cca) in southeast asia is much higher than other areas of the world. eating raw, fermented, or undercooked cyprinid fish, infected with the liver fluke, opisthorchis viverrini sensu lato (sl), results in chronic biliary inflammation, periductal fibrosis, and increased cancer risk. there may be associated glomerulonephritis. the process of infection is difficult to disrupt because eating practices have proven extremely difficult to change, and the life cycle ... | 2017 | 28848361 |
| the battle of worldviews: a case study of liver fluke infection in khon kaen, thailand. | control efforts to reduce infection from the parasitic flatworm opisthorchis viverrini have progressed through understanding the epidemiology of opisthorchis viverrini, antiparasitic drug developments, technological innovations, health education promoting cooking of fish, and improved hygienic defecation. yet the problem persists. the case study method was used to examine the fundamental cause of the liver fluke infection problem. evidence shows that the liver fluke-infected population does not ... | 2017 | 28795594 |
| prospects and challenges towards sustainable liver fluke control. | the liver fluke opisthorchis viverrini (ov) is endemic in southeast asia where more than 10 million people are estimated to be infected. the infection is associated with several hepatobiliary diseases, including cholangiocarcinoma (cca). northeast thailand is a hotspot for ov transmission, and, despite extensive public health prevention campaigns led by the government, the prevalence of ov infection is still high. high infection rates result from cultural and ecological complexities where wet-ri ... | 2017 | 28754415 |
| ultrastructural characters of the spermatozoon of the liver fluke opisthorchis viverrini (poirier, 1886) (opisthorchiidae). | the present study records the ultrastructural organization of the mature spermatozoon of opisthorchis viverrini by means of transmission electron microscopy. the spermatozoon of o. viverrini is a filiform cell, tapered at both extremities. it exhibits the characteristics of type iv spermatozoon of digeneans, namely with two axonemes of the 9+'1' trepaxonematan pattern, external ornamentation of the plasma membrane associated with cortical microtubules that are in the posterior part of the anteri ... | 2017 | 28725936 |
| subsets of inflammatory cytokine gene polymorphisms are associated with risk of carcinogenic liver fluke opisthorchis viverrini-associated advanced periductal fibrosis and cholangiocarcinoma. | opisthorchis viverrini infection induces chronic inflammation, and a minor proportion of infected individuals develop advanced periductal fibrosis (apf) and cholangiocarcinoma (cca). inflammatory cytokines and/or their gene polymorphisms may link to these biliary pathologies. we therefore investigated associations among cytokine gene polymorphisms and cytokine production in 510 thai cases infected with o. viverrini who presented with apf+ or apf-, as established by abdominal ultrasonography as w ... | 2017 | 28719954 |
| simplified techniques for killing the carcinogenic, opisthorchis viverrini metacercariae in cyprinid fish | consumption of fluke-free fish is the most important factor in controlling opisthorchis viverrini (ov) infection in endemic areas such as northeast thailand and thereby reducing the risk of cholangiocarcinoma. cooking fish is the best way to avoid infection; however, the cultural practice of eating raw or fermented fish is difficult to change. we investigated the food preparation process, using freezing, heating and fermentation to kill ov metacercariae in fish. uncooked cyprinid fish infected w ... | 2017 | 28669159 |
| present situation of opisthorchiasis in vientiane capital, lao peoples' democratic republic. | opisthorchiasis is the commonest liver disease caused by opisthorchis viverrini in thailand, lao people's democratic republic (pdr) and cambodia, which will be a major cause of future human cholangiocarcinoma in these regions. in this short review, we describe the epidemic aspects of this parasite infection and the outline of results of our recent surveys of the fluke in humans and its intermediate hosts in vientiane capital, where information on the opisthorchiasis epidemic is insufficient. we ... | 2017 | 28552889 |
| development of a potent wound healing agent based on the liver fluke granulin structural fold. | granulins are a family of protein growth factors that are involved in cell proliferation. an orthologue of granulin from the human parasitic liver fluke opisthorchis viverrini, known as ov-grn-1, induces angiogenesis and accelerates wound repair. recombinant ov-grn-1 production is complex and poses an obstacle for clinical development. to identify the bioactive region(s) of ov-grn-1, four truncated n-terminal analogues were synthesized and characterized structurally using nmr spectroscopy. pepti ... | 2017 | 28425707 |
| protein kinases as targets for opisthorchis viverrini- associated cholangiocarcinoma therapy. | protein kinases are enzymes that catalyze the transfer of phosphate from atp to the serine/threonine or tyrosine residues of target molecules. these are key important mediators in a signaling cascade involved in several biological processes. dysregulation of their activity has been found in various tumors. from our increased understanding of kinase structure and activation mechanisms there emerged new strategies for targeting kinase in cancer treatment. nowadays, kinase specific inhibitors are d ... | 2017 | 28699535 |
| nimotuzumab inhibits cholangiocarcinoma cell metastasis via suppression of the epithelial-mesenchymal transition process. | changes in epidermal growth factor receptor (egfr) are commonly found in cancer progression, signaling a poor outcome in patients. in the present study, we aimed to investigate whether nimotuzumab could be of benefit for cholangiocarcinoma (cca) treatment. | 2017 | 28668850 |
| the role of domestic dogs in the transmission of zoonotic helminthes in a rural area of mekong river basin. | dogs have been bred since ancient times for companionship, hunting, protection, shepherding and other human activities. some canine helminth parasites can cause significant clinical diseases in humans as opisthorchis viverrini causing cholangiocarcinoma in southeast asian countries. in this study, socio-cultural questionnaire, canine parasitological analysis, necropsy, parasite molecular confirmation and dog roaming data were evaluated in savannakhet, lao-pdr, a typical mekong basin area. dog ow ... | 2017 | 28426425 |
| comparison of novel and standard diagnostic tools for the detection of schistosoma mekongi infection in lao people's democratic republic and cambodia. | given the restricted distribution of schistosoma mekongi in one province in lao people's democratic republic (lao pdr) and two provinces in cambodia, together with progress of the national control programmes aimed at reducing morbidity and infection prevalence, the elimination of schistosomiasis mekongi seems feasible. however, sensitive diagnostic tools will be required to determine whether elimination has been achieved. we compared several standard and novel diagnostic tools in s. mekongi-ende ... | 2017 | 28793922 |
| lineage-specific expansion and loss of tyrosinase genes across platyhelminths and their induction profiles in the carcinogenic oriental liver fluke, clonorchis sinensis. | tyrosinase provides an essential activity during egg production in diverse platyhelminths by mediating sclerotization of eggshells. in this study, we investigated the genomic and evolutionary features of tyrosinases in parasitic platyhelminths whose genomic information is available. a pair of paralogous tyrosinases was detected in most trematodes, whereas they were lost in cyclophyllidean cestodes. a pseudophyllidean cestode displaying egg biology similar to that of trematodes possessed an ortho ... | 2017 | 28583212 |
| the complete mitochondrial genome of orthocoelium streptocoelium (digenea: paramphistomidae) for comparison with other digeneans. | orthocoelium streptocoelium is a common paramphistome species parasitizing the rumen and/or reticulum of small ruminants, leading to significant losses. this study first determined the complete mitochondrial (mt) genome of o. streptocoelium. the complete mt genome of o. streptocoelium was amplified, sequenced, assembled, analysed and then compared with those of other digeneans. the entire mt genome of o. streptocoelium is 13,800 bp in length, which is smaller than those of other digeneans except ... | 2017 | 27030161 |
| epidemiological investigation of parasitic infection of schoolchildren from six elementary schools in sakon nakhon province, thailand. | we conducted an epidemiological study of intestinal parasitic infection in 572 schoolchildren aged 4 to 12 years old from six elementary schools in sakon nakhon province, thailand from june 2013 to august 2014. we collected fecal, blood, and urine samples to investigate parasitic infection and conducted a questionnaire survey. soil samples were examined for egg contamination. fecal examination, using the formalin-ether sedimentation method, revealed that 39% of schoolchildren were infected with ... | 2017 | 28289269 |
| development of a pcr assay for diagnosing trematode (opisthorchis and haplorchis) infections in human stools. | we developed a combined conventional polymerase chain reaction (pcr) and real-time pcr (qpcr)-based assay for detecting and discriminating between opisthorchis viverrini and haplorchis taichui parasite infections. the first pcr amplifies the mitochondrial cytochrome c oxidase subunit i (coi) genes of parasites, and differential diagnosis is achieved by performing qpcr with specific primers and sybr green i. the detection limit of the assay was found to be 2.0 × 10(2) plasmid copies in a test in ... | 2017 | 27821695 |
| nitrative and oxidative dna damage in infection-related carcinogenesis in relation to cancer stem cells. | infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells, and result in the formation of oxidative and nitrative dna lesions, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in inflammation-mediated carcinogenesis. it has bee ... | 2017 | 28163802 |
| coinfection with helicobacter pylori and opisthorchis viverrini enhances the severity of hepatobiliary abnormalities in hamsters. | persistent infection with opisthorchis viverrini causes hepatobiliary abnormalities, predisposing infected individuals to cholangiocarcinoma (cca). in addition, helicobacter pylori is highly prevalent in most countries and is a possible risk factor for cca; however, its role in enhancing hepatobiliary abnormality is unclear. here, we investigated the effects of coinfection with h. pylori and o. viverrini on hepatobiliary abnormality. hamsters were divided into four groups: (i) normal, (ii) h. py ... | 2017 | 28138021 |
| opisthorchis viverrini infection in the snail and fish intermediate hosts in central vietnam. | opisthorchis viverrini, a carcinogenic fish borne fluke, requires freshwater snails and fish as intermediate hosts. opisthorchiasis is endemic in parts of southeast asia, including central and south vietnam. in this region the transmission by intermediate hosts has received little attention. therefore, freshwater snails and wild fish from bau my tho, an opisthorchiasis endemic area in binh dinh province were collected for examination of o. viverrini cercariae and metacercariae, respectively. a t ... | 2017 | 28242064 |
| preliminary genetic evidence of two different populations of opisthorchis viverrini in lao pdr. | opisthorchis viverrini is a major public health concern in southeast asia. various reports have suggested that this parasite may represent a species complex, with genetic structure in the region perhaps being dictated by geographical factors and different species of intermediate hosts. we used four microsatellite loci to analyze o. viverrini adult worms originating from six species of cyprinid fish in thailand and lao pdr. two distinct o. viverrini populations were observed. in ban phai, thailan ... | 2017 | 28238124 |
| effects of albendazole, artesunate, praziquantel and miltefosine, on opisthorchis viverrini cercariae and mature metacercariae. | to explore larvicidal effects of anthelmintic drugs on opisthorchis viverrini (o. viverrini) for alternative approach to interrupting its cycle for developing a field-based control program. | 2017 | 28237476 |
| social network analysis of food sharing among households in opisthorchiasis endemic villages of lawa lake, thailand. | consumption of raw fish is a well-documented risk factor for opisthorchis viverrini infection. sharing of food, especially raw fish recipes may influence the spread of disease through a community. using social network analysis of an ego network, we investigated food sharing among households in an opisthorchis-endemic area. network centrality properties were used to explain the differences in o. viverrini transmission and control between villages with a low and high prevalence of infection. infor ... | 2017 | 28188767 |
| efficacy and safety of praziquantel against light infections of opisthorchis viverrini: a randomized parallel single-blind dose-ranging trial. | 2017 | 28174906 | |
| targeting hexokinase ii as a possible therapy for cholangiocarcinoma. | overexpression of hexokinase 2 (hkii) has been demonstrated in various cancers. a number of in vitro and in vivo studies in several cancers show the significance of hkii in many cellular processes including proliferation, metastasis and apoptosis. however, the role of hkii in opisthorchis viverrini (ov) associated cholangiocarcinoma (cca) is still unknown. in the present study, the expression and roles of hkii were determined in ov associated cca. the expression of hkii was investigated in 82 pa ... | 2017 | 28131825 |
| efficacy of moxidectin versus ivermectin against strongyloides stercoralis infections: a randomized controlled non-inferiority trial. | infections with strongyloides stercoralis are of considerable public health relevance. moxidectin, a well-established drug in veterinary medicine under consideration for regulatory submission for the treatment of onchocerciasis, might serve as alternative to the most widely used ivermectin. | 2017 | 28369530 |
| modeling impacts of climate change on the potential distribution of the carcinogenic liver fluke, opisthorchis viverrini, in thailand. | global climate change is now regarded as imposing a significant threat of enhancing transmission of parasitic diseases. maximum entropy species distribution modeling (maxent) was used to explore how projected climate change could affect the potential distribution of the carcinogenic liver fluke, opisthorchis viverrini, in thailand. a range of climate variables was used: the hadley global environment model 2-earth system (hadgem2-es) climate change model and also the ipcc scenarios a2a for 2050 a ... | 2017 | 27774575 |
| elevated prevalence of helicobacter species and virulence factors in opisthorchiasis and associated hepatobiliary disease. | recent reports suggest that opisthorchis viverrini serves as a reservoir of helicobacter and implicate helicobacter in pathogenesis of opisthorchiasis-associated cholangiocarcinoma (cca). here, 553 age-sex matched cases and controls, 293 and 260 positive and negative for liver fluke o. viverrini eggs, of residents in northeastern thailand were investigated for associations among infection with liver fluke, helicobacter and hepatobiliary fibrosis. the prevalence of h. pylori infection was higher ... | 2017 | 28198451 |
| differential protein expression marks the transition from infection with opisthorchis viverrini to cholangiocarcinoma. | parts of southeast asia have the highest incidence of intrahepatic cholangiocarcinoma (cca) in the world due to infection by the liver fluke opisthorchis viverrini (ov). ov-associated cca is the culmination of chronic ov-infection, with the persistent production of the growth factors and cytokines associated with persistent inflammation, which can endure for years in ov-infected individuals prior to transitioning to cca. isobaric labelling and tandem mass spectrometry of liver tissue from a hams ... | 2017 | 28232516 |
| morphological and molecular identification of the liver fluke opisthorchis viverrini in the first intermediate host bithynia snails and its prevalence in kampong cham province, cambodia. | 2017 | 28189767 | |
| application of environmental dna analysis for the detection of opisthorchis viverrini dna in water samples. | opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in southeast asian countries. the trematode, opisthorchis viverrini, is the causative agent of the disease. accurate and rapid monitoring of o. viverrini is crucial for disease prevention and containment. therefore, in this study we sought to develop a novel species-specific real-time pcr assay for detecting o. viverrini using environmental dna (edna). the diagnostic sensitivity ... | 2017 | 28108370 |
| clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin β4-fak/src signaling pathway. | cholangiocarcinoma (cca) is a slow-growing but highly metastatic cancer. its metastatic potential largely explains its high mortality rate. a recognized risk factor for cca development is infection with the liver flukes opisthorchis viverrini and clonorchis sinensis. we previously reported that the excretory-secretory products (esps) of c. sinensis promoted the three-dimensional aggregation and invasion of cca cells. in the present study, a quantitative real-time pcr array of extracellular matri ... | 2017 | 28286026 |
| recurrent emergence of structural variants of ltr retrotransposon csrn1 evolving novel expression strategy and their selective expansion in a carcinogenic liver fluke, clonorchis sinensis. | autonomous retrotransposons, in which replication and transcription are coupled, encode the essential gag and pol genes as a fusion or separate overlapping form(s) that are expressed in single transcripts regulated by a common upstream promoter. the element-specific expression strategies have driven development of relevant translational recoding mechanisms including ribosomal frameshifting to satisfy the protein stoichiometry critical for the assembly of infectious virus-like particles. retrotra ... | 2017 | 28322871 |
| [control of opisthorchis viverrini infection for cholangiocarcinoma prevention]. | the international agency for research on cancer (iarc) has classified two liver flukes as carcinogenic to humans (group 1): opisthorchis viverrini in 1994 and clonorchis sinensis in 2009. this review is focused on o. viverrini, the most studied of these two trematodes, which infects nearly 10 million people in southeast asia. the life cycle involves two intermediate hosts living in fresh water: a snail of the genus bithynia and a ciprinid fish. the definitive hosts (human, cat, dog) become infec ... | 2017 | 28105582 |
| fish sharing as a risk factor for opisthorchis viverrini infection: evidence from two villages in north-eastern thailand. | foodborne trematodiasis (fbt) is a significant global health problem, with the liver flukes opisthorchis viverrini, o. felineus, and clonorchis sinensis contributing to half of the global burden of fbt. north-eastern thailand where o. viverrini is endemic and un-cooked fish dishes remain an integral part of the food culture has the highest reported incidence of opisthorchiasis, including associated cholangiocarcinoma. both food sharing and eating practices are potentially important factors in ft ... | 2017 | 28372560 |
| liver fluke infection and cholangiocarcinoma: a review. | parasites are significant groups for carcinogenesis among which liver flukes, including opisthorchis viverrini and clonorchis sinensis, are typical representatives causing cholangiocarcinoma (cca), the second most common primary hepatic malignancy with dismal prognosis. o. viverrini is prevalent in southeast asia, infecting 10 million people while c. sinensis has a wider distribution in east asia and several southeast asian countries, affecting more than 35 million people's health. these two wor ... | 2017 | 27718017 |
| role of socio-cultural and economic factors in cyprinid fish distribution networks and consumption in lawa lake region, northeast thailand: novel perspectives on opisthorchis viverrini transmission dynamics. | opisthorchis viverrini (ov) is a fish-borne parasite endemic in parts of lao pdr, cambodia, southern vietnam and northeast thailand (isaan) where an estimated 10 million people are infected. human ov infection, associated with hepatobiliary complications, including cholangiocarcinoma (cca), occurs when infected fish are consumed raw or undercooked, a longstanding cultural tradition in the region. this mixed- methods descriptive study was carried out in isaan villages around lawa lake, khon kaen ... | 2017 | 28216369 |
| new locality record for haplorchoides mehrai and possible interactions with opisthorchis viverrini metacercariae in cyprinid fishes in northeast thailand. | metacercariae of opisthorchis viverrini, a carcinogenic liver fluke, and haplorchoides sp., a trematode maturing in catfish, are commonly found in cyprinid fish, the second intermediate hosts of both flukes. however, the specific identity of haplorchoides sp. in thailand and a precise assessment of the effects of co-infections with o. viverrini have never been clarified. therefore, we aimed to identify the species of haplorchoides and to investigate possible interactions of the two trematode spe ... | 2017 | 27882434 |
| cytokine profiles in opisthorchis viverrini stimulated peripheral blood mononuclear cells from cholangiocarcinoma patients. | the carcinogenic liver fluke opisthorchis viverrini causes chronic inflammation in the bile duct and resulting in unremitting tissue damage that lead to hepatobiliary diseases, including cholangiocarcinoma (cca). despite inflammatory cytokine expression having been studied in the animal model, so far no studies have been carried out on cytokines in human cca cases. here we report the profile of cytokine production in peripheral blood mononuclear cells (pbmcs) collected from o. viverrini-associat ... | 2017 | 27769807 |
| delineating distinct heme-scavenging and binding functions of domains in mf6p/hdm proteins from parasitic flatworms. | mf6p/fhhdm-1 is a small protein secreted by the parasitic flatworm (trematode) fasciola hepatica and belonging to a broad family of heme-binding proteins (mf6p/hdms). mf6p/hdms are of interest for understanding heme homeostasis in trematodes and also as potential targets for the development of new flukicides. moreover, interest in these molecules has also increased due to their immunomodulatory properties. here we have extended our previous findings on the mechanism of mf6p/hdm-heme interactions ... | 2017 | 28348084 |
| experimental and modelling investigations of opisthorchis viverrini miracidia transmission over time and across temperatures: implications for control. | transmissibility is a significant factor in parasite fitness. the rate and magnitude of parasite transmission affect prevalence and infection intensity in individual hosts and are influenced by environmental factors. in this context, the objectives of this study were: (i) to experimentally assess opisthorchis viverrini miracidia survival and infectivity over time and across temperatures; and (ii) to combine these experimental results with environmental data to build a key component of a transmis ... | 2017 | 28237890 |
| prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower northern thailand. | gastrointestinal zoonotic helminths of dogs and cats have a public health concern worldwide. we investigated the prevalence of gastrointestinal helminths of zoonotic significance in dogs and cats in lower northern thailand and utilized molecular tools for species identification of hookworms and opisthorchis viverrini. fecal samples of 197 dogs and 180 cats were collected. overall prevalence of infection using microscopy was 40.1% in dogs and 33.9% in cats. helminth infection found in both dogs a ... | 2017 | 27570099 |