Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| iron, ferritin, hepatitis b surface and core antigens in the livers of chinese patients with hepatocellular carcinoma. | surgically resected specimens, consisting of tumor and adjacent non-neoplastic liver tissue, were obtained from 40 patients with primary liver cancer at zhong shan hospital, shanghai medical university, the people's republic of china, between march 1983 and july 1984. all were hepatocellular carcinomas (hcc), one being admixed with cholangiocarcinoma. the relationship of hepatitis b virus (hbv) markers with iron and ferritin was evaluated in liver tissues from patients with primary liver cancers ... | 1987 | 3028601 |
| isolation of two immunogenically different allergens from schistosoma japonicum eggs. | two allergenic components, termed j1 and j2, were isolated from a soluble egg antigen preparation (sea) of schistosoma japonicum by anion-exchange chromatography on de52 and gel chromatography on sephacryl s-200. the apparent molecular weights of j1 and j2 were 260,000 and 46,000, respectively, by gel chromatography on sephadex g-150. by sds-polyacrylamide gel electrophoresis, both j1 and j2 showed apparent homogenicity and their estimated molecular weights were 135,000 and 45,000, respectively. ... | 1987 | 3034785 |
| cloning of a schistosoma japonicum gene encoding a major immunogen recognized by hyperinfected rabbits. | a library of randomly sheared schistosoma japonicum genomic dna fragments was constructed in the bacteriophage expression vector lambda gt11. a portion of the library was screened with sera collected from rabbits 8 weeks after they were infected with 1000 cercariae. four clones whose recombinant gene products react with the rabbit sera were purified to homogeneity. clone sjir-12a was chosen for detailed study because of its very intense reaction with the rabbit sera. sjir-12a was found to encode ... | 1987 | 3041213 |
| schistosomal glomerulopathy: selection factors. | of several hundreds of millions of people infested with schistosomiasis, only a few hundreds have, so far, been documented to have one or other of the three schistosoma-associated immune-mediated glomerulopathies, namely proliferative glomerulonephritis, focal and segmental sclerosis, and amyloidosis. regardless of undoubted under-reporting, some factors must be involved in the selection of those who develop such glomerulopathies. on the basis of experimental and clinical evidence, this review h ... | 1987 | 3126449 |
| susceptibility of schistosoma japonicum to different developmental stages to praziquantel. | 1987 | 3127152 | |
| in vitro analysis of t-cell-mediated immunity to intact eggs in murine experimental schistosoma japonicum infection. | to characterize the mechanisms of induction and regulation of the cell population involved in granuloma formation around eggs of schistosoma japonicum, we utilized a simple method of in vitro experiments. lyt1+2-t cells were essential for in vitro responses to the intact s. japonicum eggs, which were assumed to be comparable to in vivo granulomatous responses. t-cell responses seemed to be macrophage-dependent, and responding t cells produced il-2-like activities in the culture supernatant. sera ... | 1987 | 3127618 |
| [efficacy of praziquantel silicone elastomers on animals infected with schistosoma japonicum]. | 1987 | 3128057 | |
| [histochemical studies of artemether, fuvinazole and niridazole on schistosomula of schistosoma japonicum and mouse livers]. | 1987 | 3130747 | |
| [detection of enzyme dissociated scic from sera of schistosomiasis japonica by two-direction counter-immunoelectrophoresis]. | 1987 | 3131037 | |
| [observation on the stability of schistosome egg output in final host feces]. | 1987 | 3131039 | |
| negative conversion of cop (circumoval precipitation) reactions after selective mass chemotherapy on schistosomiasis japonica with praziquantel in bohol, philippines. | for the longitudinal survey of human schistosomiasis japonica in the villages of sto. tomas and san vicente in the province of bohol, philippines, the circumoval precipitin (cop) test was used for case detection. plasma collected by finger prick from the community people and lyophilized eggs of schistosoma japonicum were used for the assay. degree of precipitation at type 2 or 3 of yokogawa et al. [1] was considered as positive. selective mass chemotherapy with praziquantel was given to the pati ... | 1987 | 3131561 |
| differential susceptibility of male and female oncomelania hupensis quadrasi infected with schistosoma japonicum. | 1987 | 3429119 | |
| the simple laboratory maintenance of a highly productive schistosoma japonicum life cycle. | the maintenance and infection of oncomelania hupensis hupensis, the intermediate host of the chinese strain of schistosoma japonicum, is described. 60% of the snails exposed to miracidia became patent, and 5000 patent snails can be produced which yield over 250,000 cercariae per week. the maintenance and infection of snails require less than 8 man-hours per week. | 1987 | 3445324 |
| effect of praziquantel on larval stages of schistosoma japonicum. | the effect of praziquantel on s. japonicum mother sporocysts, daughter sporocysts and cercariae was studied. at concentrations of 3 x 10(-7), 3 x 10(-6) and 3 x 10(-5) m and treatment times of 24 or 48 h, mother and daughter sporocysts and young cercarial embryos were not affected but nearly mature cercariae were killed and dissociated. the resistance of young cercariae could support the suggestion that the primitive cercarial epithelium arises from the sporocyst tegument. treatment with praziqu ... | 1987 | 3445349 |
| [effect of praziquantel on schistosoma japonicum cercariae]. | 1987 | 3445780 | |
| [role of respiration in the energy metabolism of schistosoma japonicum]. | 1987 | 3447776 | |
| [effects of praziquantel on the tegument of different developmental stages of schistosoma japonicum and host cell response]. | 1987 | 3447783 | |
| scanning electron microscopic study of the tegumental surface of the hybrid schistosome between schistosoma mekongi and s. japonicum-like (malaysian). | hybridization experiments between the two non-sibling species of schistosomes, schistosoma mekongi in man and s. japonicum-like (malaysian) in rodents, were carried out. two laboratory-bred snail species, tricula aperta (beta race), the snail host of s. mekongi and robertsiella kaporensis, the snail host of s. japonicum-like (malaysian), were used for the production of cercariae. cross mating between s. mekongi and s. japonicum-like (malaysian) were achieved in the laboratory by the usual proced ... | 1987 | 3448774 |
| [effects of praziquantel in vitro on adherence of neutrophils to schistosomula and killing of the worms]. | 1987 | 3451669 | |
| praziquantel: mode of action. | 1987 | 3493930 | |
| ultrastructure of skin-phase schistosomula of schistosoma japonicum by transmission electron microscopy. | 1987 | 3133177 | |
| responses in mice to sj26, a glutathione s-transferase of schistosoma japonicum worms. | the genetic variation in antibody responses of mice to glutathione s-transferase (gst) enzymes of schistosoma japonicum worms, and in particular to a mr 26,000 species termed sj26, was analysed. sera from infected mice, or mice immunized with adjuvant and an sj26 beta-galactosidase fusion protein produced in escherichia coli (sj26fp), or purified near-native recombinant sj26 produced in e. coli (rsj26), were assayed by enzyme-linked immunosorbent assay (elisa) for antibody titres to gst purified ... | 1987 | 2452132 |
| immune regulation in schistosomiasis japonica. | in schistosomiasis caused by the blood fluke, schistosoma japonicum, the major lesion responsible for clinical disease is the granuloma formed in the liver as an immune response to the eggs deposited there. in this review abram stavitsky discusses the most recent evidence for the mechanisms involved in the formation and spontaneous regression of the granulomas and presents a working hypothesis to guide future research. | 1987 | 25290435 |
| schistosome heat-shock proteins are immunologically distinct host-like antigens. | constitutively expressed schistosome homologues of heat-shock protein hsp70 elicit a dominant antibody response in humans infected with either schistosoma japonicum or schistosoma mansoni; in each case the parasite antigens are immunologically distinct and noncrossreactive. the antigenic site of the homologues is located near their carboxyl terminus where phylogenetic divergence between hsp70 proteins is greatest. nucleotide sequence comparison between these regions predicts very few amino acid ... | 1988 | 2457805 |
| generation and functional characterization of t cell lines and clones specific for schistosoma japonicum egg antigen in humans. | t cell lines specific for schistosoma japonicum egg ag were established in vitro from patients with chronic schistosomiasis japonica, and investigated their possible immunopathologic roles by testing lymphokines production and in vitro granuloma formation assay. all lines tested had surface phenotypes of cd3+ cd4+ cd8-, and showed s. japonicum soluble egg ag (sea)-specific proliferation requiring hla-dr-restricted ag presentation. of these fractions of sea separated by gel filtration, fraction i ... | 1988 | 2459210 |
| schistosoma japonicum egg antigen-specific t cell lines in man. induction of helper and suppressor t cell lines and clones in vitro in a patient with chronic schistosomiasis japonica. | t cell lines (tcls) specific for schistosoma japonicum egg antigen were established from a patient with chronic schistosomiasis japonica to investigate the regulatory mechanism of s.japonicum egg antigen-driven t cell responses in man. all five tcls tested were cd2+, cd4+, cd8-, and were strongly proliferative only to s. japonicum egg antigen in the absence of exogenous il-2. all but one tcl produced il-2-like lymphokines in vitro, indicating their helper t cell functions. one tcl, sje-3, failed ... | 1988 | 2449460 |
| [preliminary report on the diagnosis of schistosomiasis japonica by the indirect fluorescent antibody test and immunoenzyme staining technic]. | 1988 | 3133180 | |
| immunoblotting analysis of the major integral membrane protein antigens of schistosoma japonicum. | triton x-114 has been employed to isolate integral membrane proteins from schistosoma japonicum and schistosoma mansoni adult worms. suitable marker molecules and antisera directed or raised against schistosome proteins partitioned by triton x-114 extraction indicated that the phase separation and purification of integral membrane proteins had been successful and this fraction was free of contamination with aqueous (soluble) or secretory antigens. two dimensional immunoblots further exemplified ... | 1988 | 3133562 |
| schistosomiasis japonica: epidemiology and genetic aspects. | 1988 | 3136552 | |
| worm burden and lymphocyte response in mice immunized with n-methyl-n'-nitro-n-nitrosoguanidine attenuated cercariae of schistosoma japonicum. | 1988 | 3136992 | |
| immunologic diagnosis of schistosomiasis japonica by indirect immunoperoxidase on liver-egg section (le-iip). | 1988 | 3136993 | |
| correlation between worm burden and the incidence of circulating anodic antigens or antibodies in mice infected with schistosoma japonicum or schistosoma mansoni. | 1988 | 3141678 | |
| hla-typing in schistosoma japonicum infection. | 1988 | 3142119 | |
| [effects of praziquantel on the immunological response in mice infected with schistosoma japonicum]. | 1988 | 3142211 | |
| [effect of praziquantel on ultrastructure of schistosoma japonicum cercariae and schistosomula within mouse skin]. | 1988 | 3143224 | |
| molecular and serological characteristics of the glutathione s-transferases of schistosoma japonicum and schistosoma mansoni. | when aqueous extracts of schistosoma japonicum and s. mansoni adult worms are passed over columns of glutathione-conjugated agarose, two molecular species of mr 26,000 and mr 28,000 are detected in eluates as analysed by sds-page, these eluates having glutathione s-transferase (gst) activity. the molecules, termed sj26 and sj28 from s. japonicum and sm26 and sm28 from s. mansoni, can be immunogenic in rabbits or mice and appear not to be linked together as subunits of gst heterodimers. the eluti ... | 1988 | 3146049 |
| [study on circumoval precipitin test using dry egg antigen smear on pvc membrane in the diagnosis of schistosomiasis japonica]. | 1988 | 3149552 | |
| [a preliminary study on applicability of latex agglutination test in schistosomiasis-eradicated area]. | 1988 | 3149555 | |
| [diagnostic proteins of 31/31 kd in schistosoma japonicum: reactivity with patient sera and monoclonal antibodies]. | 1988 | 3150946 | |
| [the reconformation of the existence of lithoglyphopsis modestus (gredler, 1886) in hunan province, china and its artificial infection with schistosoma japonicum]. | 1988 | 3150947 | |
| [a computer programme for automatic recognition of schistosome eggs]. | 1988 | 3150948 | |
| schistosoma japonicum (chinese): changes of the tegument surface in cercariae, schistosomula and juvenile parasites during development. | 1988 | 3220650 | |
| high-molecular-weight eosinophil chemotactic factor from schistosoma japonicum eggs. | 1988 | 3226304 | |
| [isolation and identification of schistosoma japonicum messenger ribonucleic acid]. | 1988 | 3242967 | |
| studies on antigenic communities between the yamanashi and chinese strains of schistosoma japonicum eggs and oncomelania snails by immunoelectrophoresis. | 1988 | 3243720 | |
| [studies on adenylate cyclase in schistosoma japonicum]. | 1988 | 3253028 | |
| peculiarities of mekong schistosomiasis with particular attention to the people's democratic republic of laos. | the authors examined 10 children with clinical symptoms suggestive of intestinal or hepatolienal schistosomiasis. all the children were from the endemic focus in laos. we found eggs of schistosomes that were smaller and rounder (60.95 x 51.55 microns) than those from a southern chinese strain of s. japonicum (62.64 x 73.49 microns). there were no oncomelania species found among the snails in this endemic area. these observations confirm those reports in the literature that assert a new species f ... | 1988 | 3270583 |
| expression of an enzymatically active parasite molecule in escherichia coli: schistosoma japonicum glutathione s-transferase. | the nh2-terminal amino acid sequence of the mr 26 000 glutathione s-transferase (ec 2.5.1.18) of schistosoma japonicum (sj26) has been deduced by rna and protein sequence analysis. using this information, a bacterial plasmid has been constructed that directs the synthesis of the entire sj26 molecule in escherichia coli. recombinant sj26 exhibits glutathione s-transferase activity and can be readily purified from bacteria in a one-step procedure under non-denaturing conditions. the availability o ... | 1988 | 3278228 |
| schistosoma japonicum and paragonimus ohirai: antagonism between s. japonicum and p. ohirai in oncomelania nosophora. | antagonistic interactions between schistosoma japonicum and paragonimus ohirai were examined in the snail host, oncomelania nosophora. when p. ohirai-infected snails were exposed to s. japonicum miracidia at intervals of 4 to 18 weeks post-first exposure, only a few snails (0-7%) were found to be superinfected with s. japonicum sporocysts. sporocysts were fewer in number than those of single infected controls. mature s. japonicum cercariae were not observed. furthermore, when the snails were exa ... | 1988 | 3338543 |
| schistosoma malayensis n. sp.: a schistosoma japonicum-complex schistosome from peninsular malaysia. | schistosoma malayensis n. sp., a member of the schistosoma japonicum complex is described from rattus muelleri in peninsular malaysia and 2 strains are characterized. the only morphological differences noted among adults from natural hosts were that s. malayensis are in general smaller than s. mekongi and s. japonicum. but these differences may be the result of host-induced variations and therefore are of little taxonomic value. to minimize the effects of host-induced variations, adult worms rec ... | 1988 | 3379527 |
| immune responses in mice infected with schistosoma japonicum and treated with praziquantel in special reference to the role of egg antigen. | the sequential change of class-specific antibodies to antigens of the schistosoma japonicum egg and adult worm in mice after treatment with a single oral dose of praziquantel (pzq) at 100 mg/kg was studied using elisa in regard to the resulting release of egg antigen. in 3 out of 4 mice treated with pzq, only igm antibody to egg antigen was drastically declined from 30 min to 6 hr after treatment, and then gradually rose to the initial level after 2 to 3 days. after adding 100 micrograms/ml pzq ... | 1988 | 3411793 |
| [the application of the indirect fluorescent antibody test (ifat) in epidemiological surveys on schistosomiasis japonica]. | 1988 | 3133118 | |
| [evaluation of urea-soluble egg antigen (jeu) in the diagnosis and assessment of cure of schistosomiasis japonica]. | 1988 | 3133133 | |
| lack of effect of unmated schistosomes on the fecundity of mated worm pairs. | an imbalance between the numbers of male and female worms had no significant effect upon the fecundity of schistosoma mansoni in rhesus monkeys or upon the number of s. mansoni eggs in the tissues of infected persons or mice. the number of s. japonicum eggs in the tissues of infected rabbits was similarly unaffected by disproportion between worm genders. | 1988 | 3128653 |
| [susceptibility of the chinese hamster (cricetulus griseus) to parasitic infection. (2) experimental infection with schistosoma japonicum]. | chinese hamsters (cricetulus griseus milne-edwards: cha colony) were experimentally infected with schistosoma japonicum cercariae by the percutaneous route and intraperitoneal inoculation to confirm their susceptibility to the infection with this parasite in comparison with that of mice (dd). chinese hamsters proved to be highly susceptible to percutaneous and intraperitoneal infection with s. japonicum. the eggs of s. japonicum were recognized in feces of all chinese hamsters and mice from abou ... | 1988 | 3129351 |
| single-step purification of polypeptides expressed in escherichia coli as fusions with glutathione s-transferase. | plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in escherichia coli as fusions with the c terminus of sj26, a 26-kda glutathione s-transferase (gst; ec 2.5.1.18) encoded by the parasitic helminth schistosoma japonicum. in the majority of cases, fusion proteins are soluble in aqueous solutions and can be purified from crude bacterial lysates under non-denaturing conditions by affinity chromatography on immobilised glutathione. using batch wash pr ... | 1988 | 3047011 |
| epidemiologic studies on schistosomiasis japonica in a newly found habitat of oncomelania snails in japan. | epidemiologic studies' on schistosomiasis japonica were carried out in the obitsu river basin of central boso peninsula, a newly found habitat of oncomelania snails in japan. forty-five (4.1%) of 1,102 schoolchildren and 64 (10.4%) of 618 junior high school students were positive for the skin test with veronal buffered saline-extracted schistosoma japonicum antigen. none was positive by the enzyme-linked immunosorbent assay, complement fixation test, double diffusion test in agar, and circumoval ... | 1988 | 3124649 |
| praziquantel pharmacokinetics and side effects in schistosoma japonicum-infected patients with liver disease. | praziquantel undergoes extensive first-pass hepatic biotransformation, but there is little information on its disposition or toxicity when administered to patients with liver disease. to define the influence of liver disease on the pharmacokinetics of praziquantel, we administered it orally to 30 patients with proven schistosoma japonicum infection whose liver disease was carefully assessed as being severe, moderate, or absent. both the peak plasma concentration of praziquantel and the bioavaila ... | 1988 | 3125260 |
| deficiency of interleukin-2 activity upon addition of soluble egg antigen to cultures of spleen cells from mice infected with schistosoma japonicum. | schistosoma japonicum-infected c57bl/6 mice show similar dynamics of hepatic granulomatous inflammation (hgi) and delayed hypersensitivity (dh) elicited by soluble egg antigens (sea) which reach peak levels at 9 weeks of infection and then spontaneously regress. the in vitro sea-induced proliferation of spleen cells (sc) from infected animals attained its high point and then declined when sc from 5-week-infected mice were used. the present study determined the dynamics of interleukin-2 (il-2) pr ... | 1988 | 2968321 |
| schistosoma mansoni and s. japonicum worm numbers in 129/j mice of two types and dominance of susceptibility in f1 hybrids. | in a study on the genetics of resistance to schistosomiasis in wehi 129/j mice, susceptibility to either schistosoma mansoni or schistosoma japonicum was shown to be unequivocally dominant in f1 hybrid crosses between genetically resistant wehi 129/j and susceptible balb/c mice. the operation of only 1 or 2 genes in the expression of resistance to s. mansoni was suggested by backcross analysis. thus, approximately 25% of (balb/c x wehi 129/j) f1 x wehi 129/j mice were resistant to s. mansoni inf ... | 1988 | 2969409 |
| hepatoma formation in ddy mice with chronic schistosomiasis japonica. | three hundred and ninety-five four-week-old spf female ddy mice were each exposed to 5 or 6 cercariae of schistosoma japonicum (japanese strain) on their shaved abdomens and were maintained in a conditioned clean environment and fed on sterilized food and water. fecal examinations at 8 to 10 weeks postinfection (pi) revealed 169 mice to be infected. more than half of them died within 30 weeks pi and 70 mice that survived to the 50th week pi were sacrificed. at autopsy, we could find no schistoso ... | 1988 | 3130351 |
| an outbreak of schistosomiasis japonica in taiwan. | the present study was undertaken to determine the current status of schistosoma japonicum infection in the newly found endemic focus at hanpao farm, fangyuan district, changhua county, west-central taiwan. two field surveys and one laboratory investigation were conducted from july to october 1987. of 705 snails examined by shedding and crushing methods, 17.0% were found to harbor cercariae of s. japonicum. by sodium sulfate-acid-ether-triton centrifugal sedimentation technique, 25% of 16 cattle, ... | 1988 | 3151482 |
| sensitization against the parasite antigen sj26 is not sufficient for consistent expression of resistance to schistosoma japonicum in mice. | mice immunized with purified antigen preparations produced in escherichia coli and containing the glutathione s-transferase (gst) isoenzyme of schistosoma japonicum (sj26) can be partially resistant to infection with this parasite. maximum resistance was approximately 50% and no protection was obtained in balb/c mice, known low responders to sj26. although only freund's complete adjuvant has been used, the data obtained indicate that satisfactory levels of resistance to s. japonicum will not be ... | 1988 | 3151530 |
| serological evaluation of ultrasound examination for chronic schistosomiasis japonica in a previously endemic area--the chikugo river basin, japan. | ultrasound diagnosis of chronic schistosomiasis japonica was assessed by comparison with the results of serological tests using enzyme-linked immunosorbent assay (elisa) with egg and adult worm antigen, and the circumoval precipitation test. the subjects were persons resident in the chikugo river basin in kyushu, southwest japan, where schistosomiasis used to be highly prevalent. of 93 individuals with a past history of infection, 37 (39.8%) presented the characteristic echo pattern of the disea ... | 1988 | 3151543 |
| granulomatous response in selective ige-deficient sja/9 mice infected with schistosoma japonicum. | granulomatous response was examined in selective ige-deficient sja/9 mice infected with schistosoma japonicum. the average size of granulomas formed around newly deposited eggs in sja/9 mice was significantly smaller than that in control c57bl/6 or sjl/j mice, although the numbers of adult worms recovered from each strain were comparable among them. thus, ige antibody specific to allergenic components of s. japonicum eggs seems to act as an amplifier for the formation of granulomatous lesions. | 1989 | 2592120 |
| induction of resistance in oncomelania hupensis nosophora against schistosoma japonicum, but not against paragonimus ohirai, using irradiated miracidia. | oncomelania hupensis nosophora snails sensitized with x-irradiated schistosoma japonicum miracidia demonstrated resistance against a following challenge infection with non-irradiated homologous miracidia. the resistance in o. h. nosophora against s. japonicum was acquired within 1 day of sensitization, and it was strongest in a group challenged at an interval of 3 days. the resistance persisted for at least 4 weeks. histological examinations revealed amoebocyte accumulation around the challenged ... | 1989 | 2592137 |
| [synthesis of acylates of niridazole and its analogs as schistosomicides]. | in order to decrease the toxicity and enhance the curative effect of niridazole against schistosomiasis japonica, a series of acylates of niridazole has been prepared through acylation of niridazole, 2-substituted acetamido-5-nitrothiazoles and 1-(5-nitro-2-thiazolyl)-4-acylpiperazine were also prepared. the products has been tested against schistosomiasis japonica in mice. preliminary test results showed that the majority of aliphatic acylates of niridazole exhibited marked schistosomicidal eff ... | 1989 | 2609988 |
| histochemical evidence of the catecholamine-associated nervous system in certain schistosome cercariae. | the localization of catecholamines was documented in the cercaria of schistosoma mansoni and s. japonicum by a fluorescent histochemical method using glyoxylic acid (ga). cell bodies and nerve fibres were spatially visualized in whole-mount preparations, and the fluorescent traces were investigated. the nervous system was bilaterally symmetrical, showing a similar formation in both species. a pair of cerebral ganglia with a transverse commissure showed a complex network of fluorophores, and each ... | 1989 | 2616566 |
| glucose metabolism of adult schistosoma japonicum as revealed by nuclear magnetic resonance spectroscopy with d-[13c6]glucose. | excretory end-products of adult schistosoma japonicum, fed d-[13c6]glucose in vitro under aerobic and anaerobic conditions, were studied using 1h- and 13c-nuclear magnetic resonance (nmr) spectroscopy. the glucose in the medium is degraded to produce lactate and alanine aerobically and succinate and acetate as well as lactate and alanine anaerobically. succinate and acetate have not been previously recorded as excretory products resulting from the metabolism of glucose for schistosomes. the pres ... | 1989 | 2616648 |
| schistosoma japonicum: the effect of serum fractions precipitated by ammonium sulfate on viability of schistosomula. | the effect of serum fractions precipitated by a saturated ammonium sulfate solution on viability of schistosoma japonicum schistosomula was investigated. axenized cercariae were incubated for 10 days at 37 degrees c in nctc 109 supplemented with the 34%, 40%, 50%, 62% and 68% fractions of human or rabbit serum in an equivalent concentration to 10% serum. only larvae cultured in media with the 68% fraction of human or rabbit serum retained the transparent feature and active motility, while the la ... | 1989 | 2622065 |
| human chorionic gonadotropin-like immunoreactivity in schistosomes and fasciola. | 1989 | 2622900 | |
| vaccination against ovine cysticercosis using a defined recombinant antigen. | cysticercosis caused by larval tapeworms is a major public health problem and a cause of substantial economic losses in the farm-animal industries. taenia ovis in sheep is a particularly important example. immunity to reinfection with the larvae has a central role in regulating natural transmission of the parasites, and vaccination with antigens from the early larval oncosphere stage can induce complete protection against infection. as it is impractical to obtain enough oncospheres for a commerc ... | 1989 | 2648160 |
| characterization of a large gene family in schistosoma japonicum that encodes an immunogenic miracidial antigen. | three of eleven clones isolated from a genomic expression library of schistosoma japonicum dna using chronically infected human sera also react with chronically infected mouse sera. characterization of these three clones showed that they contain different members of the same gene family. one clone contains two members of the gene family approximately 2 kb apart and in opposite orientation to each other. dna sequence homologies between pairs of genes range from 98% to 99.5%. southern hybridizatio ... | 1989 | 2657419 |
| soluble proteins from schistosoma mansoni and japonicum: a comparative biochemical and immunological analysis. | 1. soluble proteins were recovered from male schistosoma mansoni after homogenization in tris-hcl buffer containing 0.6 m kcl and 1.0% triton x-100 followed by preparative electrophoresis on sds-gel. 2. polyclonal antibodies produced in mice against the soluble fraction were used in comparative analysis of s. mansoni and s. japonicum using immunoblots and immunoprecipitation of in vitro translated polypeptides. 3. small molecular weight polypeptide (20-22 kdalton), identified by infected mouse s ... | 1989 | 2667879 |
| [advances in research on the culturing of schistosoma japonicum in vitro]. | 1989 | 2670314 | |
| [production and characterization of monoclonal antibodies to echinococcus multilocularis]. | spleen cells from balb/c mice immunized against echinococcus multilocularis were fused with mouse myeloma cells (p3x6.5.3.) in the presence of polyethylene glycol (peg 1000), and the hybridomas were obtained. of the 85 hybridomas, 23 were found to produce antibodies. of the 23 cell lines, 13 hybridomas which produced a high titer of antibodies were cultured for cloning the cells by the limiting dilution method. then 5 monoclonal antibodies were obtained. the immunoglobulin class of the monoclona ... | 1989 | 2684834 |
| comparison of morphology, pathogenicity and drug response among three isolates of schistosoma japonicum in the mainland of china. | geographic differences among isolates of schistosoma japonicum from anhui province (a) in the east, guangxi autonomous region (g) in the south and yunnan province (y) in the southwest of mainland of china were studied. c57bl mice were used to compare these isolates. morphologically, both male and female worms of isolate y were shorter than those of isolates a and g, and the ovary of female worm of isolate g was the smallest. in isolate a, the percentage of male worms bearing 7 testes was 87%, wh ... | 1989 | 2735700 |
| screening for molluscicidal activity in crude drugs. | thirty-four extracts of crude drugs and medicinal plants have been screened for activity against oncomelania nosophora, the intermediate host of the japanese strain of schistosoma japonicum. strong molluscicidal activity was found in the meoh extract of anemarrhenae rhizoma. although timosaponin a-iii, one of the main saponins of anemarrhenae rhizoma, showed very strong killing activity, markogenin-3-o-beta-d-glucopyranosyl (1-2)-o-beta-d-galactopyranoside having the same glycosidic linkage was ... | 1989 | 2766410 |
| carbohydrate metabolism in adult schistosomes of different strains and species. | in this comparative study the carbohydrate metabolism of schistosoma japonicum, s. haematobium, s. intercalatum, s. bovis and three strains of s. mansoni was investigated. no large differences were found in the protein and glycogen contents of all species involved. in all species investigated, lactate was the main end product of carbohydrate breakdown. however, all parasites degraded part of the glucose to co2 via the krebs cycle. no difference was observed in the contribution of this aerobic pr ... | 1989 | 2777465 |
| [a study on mechanism of skin penetration by cercaria of s. japonicum and screening of preventive drugs]. | mice pretreated with pg cyclo-oxygenase inhibitor aspirin, at 300 mg/kg/d x 5, and ibuprofen, at 100 mg/kg/d x 5 orally and 20 mg/kg/d x 3 intraperitoneally revealed moderate chemoprophylactic effects and the reduction of the penetration of worms by 23% when compared to controls. daily oral administration of aspirin, at 300 mg/kg for 15 days the worms reduction increased to 41.7%. in estimation of pge1 in cercariae of s. japonicum by radioimmunoassay, the pge1 was 49 pg/100 cercariae. in normal ... | 1989 | 2793135 |
| integration and expression of murine retrovirus-related sequences in schistosomes. | antibodies against the retrovirus envelope glycoprotein (gp70) of mouse xenotropic retrovirus, balb virus 2 (bv2) reacted with the adult worms of schistosoma japonicum and s. mansoni. this reaction was completely inhibited after adsorption of the antibodies with virions of retrovirus. the reactive schistosome antigen was located in the subtegumental layer of the adult male fluke and in the vitelline gland of the adult female of s. japonicum and s. mansoni. proteins extracted from both parasites ... | 1989 | 2797870 |
| fl-160. a surface antigen of trypanosoma cruzi that mimics mammalian nervous tissue. | chagas' disease, caused by trypanosoma cruzi, is an excellent model for autoimmune disease induced by an infectious agent. transfer of t cells, directed against crossreactive antigens of t. cruzi and nervous tissue, have been shown to reproduce pathology found in chronic chagas' disease. we used recombinant dna technology to characterize one of these crossreactive antigens (fl-160). we have cloned dna from t. cruzi, which expresses a protein corresponding to a 160-kd protein found on the surface ... | 1989 | 2466939 |
| monoclonal antibodies reacting with schistosoma japonicum eggs and their target epitopes. | ten monoclonal antibodies (mcabs) raised to schistosoma japonicum eggs could be assigned using several serological and immunochemical techniques to 3 groups. the mcabs, termed a, b and c-mcabs, apparently recognize carbohydrate epitopes that can be located on the same antigen molecule. the antibodies, generally of igm isotype, are idiotypically related. they are distinct from another igm mcab (group d-mcab) the carbohydrate target epitope of which can also be associated with the epitopes of a, b ... | 1989 | 2468264 |
| reactivity of anti-tegument monoclonal antibodies with target epitopes in different worm tissues and developmental stages of schistosoma mansoni. | sixteen monoclonal antibodies (mabs) were selected for their reactivity with adult schistosome tegument. the distribution of target epitopes in different tissues of the adult and in various developmental stages was investigated by indirect immunofluorescence. the distinct patterns of reactivity of the mabs permitted their classification into 9 groups. the distribution of epitopes in larvae, particularly 3 h schistosomula, generally mirrored that in adults. a change in distribution of epitopes co ... | 1989 | 2474789 |
| granuloma formation in schistosoma japonicum infected nude mice: the effects of reconstitution with l3t4+ or lyt2+ splenic cells. | the role of l3t4+ and lyt2+ lymphocytes in the formation and modulation of granulomas around schistosoma japonicum eggs was examined in athymic mice. nude c57bl/6 mice infected with s. japonicum miracidia were compared to nude mice given normal spleen cells depleted of l3t4+ or lyt2+ cells prior to infection and to intact mice. nude mice formed small granulomas that were poor in eosinophils and connective tissue. nude mice reconstituted with cells enriched for l3t4+ or lyt2+ cells formed granulo ... | 1989 | 2492779 |
| [schistosomiasis]. | schistosomiasis ranks among the major public health problems in the tropics and subtropics. the disease is caused by the parasite schistosoma, a flatworm with a life cyclus including man as the definitive host and a freshwater snail as the intermediate host. during the last ten years one hundred cases of this disease have been reported in norway. we report two cases of schistosomiasis, one patient infected with schistosoma japonicum, the first reported case in norway, the other with schistosoma ... | 1989 | 2493686 |
| schistosome vaccines: current progress and future prospects. | vaccination against human schistosomes in laboratory hosts is now a reality. a number of different parasite molecules have been shown to confer partial protective immunity against challenge infection with schistosoma mansoni or schistosoma japonicum in rodent or primate hosts. these antigens are unusually diverse in their structure and stage specificity. interestingly, although all of the vaccine molecules characterized are situated in the tegument, their exposure on the parasite surface, in mos ... | 1989 | 2498828 |
| evidence of triple infections with schistosoma japonicum, s. haematobium, and s. mansoni in two siblings. | 1989 | 2499639 | |
| persistence of hepatic fibrosis and tissue eggs following treatment of schistosoma japonicum infected mice. | the persistence of hepatic fibrosis and of schistosoma japonicum eggs in the tissues of mice was examined after chemotherapy. c57bl/6 mice infected with a philippine strain of s. japonicum were treated with praziquantel or amoscanate 7 or 8 weeks after infection. groups of mice were killed at the time of treatment and 3, 8, 26, and 52 weeks later. the number of eggs per worm pair in the tissues did not change during the year after treatment. however, s. japonicum eggs injected into the tail vein ... | 1989 | 2500856 |
| schistosomiasis associated with rupture of the appendix in pregnancy. | acute appendicitis is among the most common indications for exploratory laparotomy during pregnancy. although usually pyogenic in origin, parasitic infections account for a small percentage of cases. we report here the association of pregnancy and appendicitis caused by schistosoma japonicum. schistosomiasis is a very common complication of pregnancy, with 250,000,000 persons infected worldwide, including 20% of pregnant women living in hyperendemic areas. schistosome egg masses can lodge throug ... | 1989 | 2503794 |
| mammographic microcalcifications associated with schistosomiasis. | we have described the first known occurrence of ectopic schistosoma japonicum of the breast mimicking the mammographic pattern of carcinoma. | 1989 | 2503893 |
| [dermal responses of various hosts to infection with schistosoma japonicum cercariae]. | the present investigation concerns with the dermal reactions produced in various host species by the invading schistosomula in primary infection. eight animal species including mouse, rat, hamster, jird, guinea pig, rabbit, rhesus monkey and pigeon were used. a small area of abdominal skin exposed to 300 schistosoma japonicum cercariae was taken at different time intervals from each animal species. | 1989 | 2504510 |
| a comparison of the antischistosomal effect of levo- and dextro-praziquantel on schistosoma japonicum and s. mansoni in mice. | praziquantel (pzq) is a racemic compound composed of equal proportions of its optical isomers, levo- and dextro-pzq. the efficacy of these compounds was compared with that of pzq in mice infected with schistosoma japonicum or s. mansoni. mice were given 50, 2 x 50 (on consecutive days), 500, or 2 x 250 mg/kg of each compound orally 5 weeks after infection. significant reduction of worm recovery was observed in s. japonicum infection 30 days after treatment with 2 x 50, 500, or 2 x 250 mg/kg of l ... | 1989 | 2505624 |
| schistosoma japonicum and s. mansoni: ultrastructural damage in the tegument and reproductive organs after treatment with levo- and dextro-praziquantel. | ultrastructural observations were made of changes in the tegument and reproductive organs of schistosoma japonicum and s. mansoni from icr mice after treatment with praziquantel (pzq), levo-pzq, and dextro-pzq at a single oral dose of 500 mg/kg body weight. no marked difference in types and extent of lesions of the tegument of s. japonicum was found between the compounds regardless of the time of worm recovery after treatment. this was equally true of s. mansoni. degeneration of the testis, ovar ... | 1989 | 2505625 |
| disappearance of specific antibodies in patients with chronic schistosomiasis japonica by treatment with praziquantel. | we tested effects of praziquantel, an antischistosomal compound, on clinical and immunological parameters of chronic schistosomiasis japonica. two japanese patients, who had high antibody titers to schistosoma japonicum antigens but no fecal schistosome eggs or no or mild symptoms complained, were treated with praziquantel. within two years after treatment, anti-schistosome antibodies in sera from the patients became negative in enzyme-linked immunosorbent assay. there was no significant alterat ... | 1989 | 2506387 |
| [further study on the immunity induced by mutagenic ntg attenuated cercariae of schistosoma japonicum]. | in order to understand the immunity induced by ntg attenuated cercariae of s. japonicum, investigations were carried out on the survival rate of mice, worm reduction rate, liver and spleen granulation etc. at different intervals between immunization and challenge (4, 6, 8, 10 and 12 wk) in mice exposed percutaneously to ntg attenuated cercariae (30 micrograms/ml ntg for 15 min). scanning electron microscopy on tegumental surface of schistosomes from immunized mice was also performed. results sho ... | 1989 | 2509101 |
| reduced levels of mutagen processing potential in the schistosoma japonicum-infected mouse liver. | epidemiological studies have shown the presence of a positive correlation between the infection of schistosoma japonicum and colorectal and/or liver cancers in the humans. to explore the mechanism underlying this correlation, we have investigated the mutagen-activating potentials of the liver homogenate fraction (s9) from schistosoma japonicum infected mice and those from control mice, by use of the ames test with 2-acetylaminofluorene, aflatoxin b1 and 3-amino-1-methyl-5h-pyrido[4,3-b]indole (t ... | 1989 | 2509904 |
| [a case of metastatic lung tumor of the colon cancer with ova of schistosoma japonicum in the resected lung specimen]. | a 69-year-old man underwent right middle lobectomy because of the tumor lesion in the right middle lung. he had ever been in celebes and china (riverside area of the yangtze), the infected area of schistosomiasis japonica, for military service during the world war ii. resected lung specimen was carefully examined at the department of pathology in our hospital. several ova of schistosoma japonicum were discovered scattered in the specimen, and the pathological feature of the lung lesion was revea ... | 1989 | 2512405 |
| schistosomiasis on bohol island, philippines, with special emphasis on the successful discovery of new habitats of the vector snail, oncomelania quadrasi, and area-wide mollusciciding. | in the endemic area of schistosomiasis on bohol island, we succeeded in locating seven new colonies of the vector snail, oncomelania quadrasi, four in trinidad and three in talibon, in addition to six locations from which the snails were previously recorded. we were led by the proximity of the snail habitat to the residence of the infected individuals. in northern bohol the continuous wet season would appear to make the major differences between the places that do and those that do not support o ... | 1989 | 2513438 |
| cross protection between a laboratory passaged chinese strain of schistosoma japonicum and field isolates of s. japonicum from china. | our laboratory strain of schistosoma japonicum has been isolated for 51 years, but is comparable to the indigenous chinese parasite in terms of its infectivity to both the intermediate and definitive hosts. vaccination with our strain protects mice against challenge with wild isolates of s. japonicum from china. thus any defined antigen vaccine developed using our laboratory strain would be expected to protect against s. japonicum in the field in china. | 1989 | 2513672 |