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ostreopexin: a hemopexin fold protein from the oyster mushroom, pleurotus ostreatus.proteins with hemopexin repeats are widespread in viruses, prokaryotes and eukaryotes. we report here for the first time the existence of a protein in fungi with the four-bladed β-propeller fold that is typical for hemopexin-like proteins. this protein was isolated from the edible basidiomycetous fungus pleurotus ostreatus and is named ostreopexin. it binds to ni(2+)-nta-agarose, and is structurally and functionally very similar to pa2 albumins isolated from legume seeds and the hemopexin fold p ...201323567905
characterization and lectin microarray of an immunomodulatory heteroglucan from pleurotus ostreatus mycelia.glucans isolated from various mushroom and mycelia sources are interestingly being studied nowadays as a potent therapeutic agent. the present work was focused on the isolation, characterization and immunomodulatory study of a novel water soluble glucan from the pure mycelia of pleurotus ostreatus. the extracted glucan was found to have a high molecular weight of ∼2.7 × 10(6)da and mainly comprised of glucose, mannose and fucose in a ratio of 3:2:1 with both β and α linkages. presence of termina ...201323544643
cadmium and lead bioavailability and their effects on polycyclic aromatic hydrocarbons biodegradation by spent mushroom substrate.bioremediation of mixed metal-organic soil pollution constitutes a difficult task in different ecosystems all around the world. the aims of this work are to determine the capacity of two spent mushroom substrates (agaricus bisporus and pleurotus ostreatus) to immobilize cd and pb, to assess the effect of these metals on laccase activity, and to determine the potential of spent a. bisporus substrate to biodegrade four polycyclic aromatic hydrocarbons (pah): fluorene, phenanthrene, anthracene, and ...201323716079
heat treatment of wheat straw by immersion in hot water decreases mushroom yield in pleurotus ostreatus.the oyster mushroom, pleurotus ostreatus, is cultivated worldwide. it is one of the most appreciated mushrooms due to its high nutritional value. immersion of the substrate in hot water is one of the most popular and worldwide treatment used for mushroom farmers. it is cheap and easy to implement.201323266315
immunomodulatory effect of pleuran (β-glucan from pleurotus ostreatus) in children with recurrent respiratory tract infections.recurrent respiratory tract infections (rrtis) represent a very important problem in daily clinical practice because of their significant contribution to morbidity in children. several natural nutritional supplements have been used in the prevention of rrtis, but the clinical efficacy of only a few preparations is supported by scientific evidence.201323261366
diversity of trichoderma spp. causing pleurotus green mould diseases in central europe.the present study includes the molecular characteristics of trichoderma pleurotum and trichoderma pleuroticola isolates collected from green moulded cereal straw substrates at 47 oyster mushroom farms in poland. the screening of the 80 trichoderma isolates was performed by morphological observation and by using the multiplex pcr assay. this approach enabled specific detection of 47 strains of t. pleurotum and 2 strains of t. pleuroticola. initial identifications were confirmed by sequencing the ...201323192526
evaluation of antidiabetic potential of oyster mushroom (pleurotus ostreatus) in alloxan-induced diabetic mice.to study the antidiabetic activity of pleurotus ostreatus in normal and alloxan-induced diabetic mice.201322867521
a heteroglycan from the mycelia of pleurotus ostreatus: structure determination and study of antioxidant properties.a heteroglycan (ps) isolated from the mycelia of pleurotus ostreatus was found to consist of l-fucose, d-mannose, and d-glucose in a molar ratio of nearly 1:2:3. on the basis of acid hydrolysis, methylation analysis, periodate oxidation, and nmr studies (1h, 13c, tocsy, dqf-cosy, noesy, roesy, hsqc, hmbc, and dept), the structure of the repeating unit of the ps was determined. the repeating unit had a branched backbone composed of (1→6)-linked α and βd-glucose and (1→2)-linked α-l-fucose. branch ...201323314300
molecular characterization of a lipoxygenase from the basidiomycete mushroom pleurotus ostreatus.the full-length cdna of the gene polox1 encoding a lipoxygenase (lox) and its corresponding genomic dna were isolated from the basidiomycete mushroom pleurotus ostreatus strain h1. the deduced amino acid sequence of polox1 showed similarity to a valencene dioxygenase of pleurotus sapidus, putative lox-like proteins from ascomycete, basidiomycete, and deuteromycete fungi, and known loxs from plants, animals, and bacteria. polox1 also contained the lox iron-binding catalytic domain in the c-termin ...201323291746
characterization of pleurotus ostreatus biofilms by using the calgary biofilm device.the adequacy of the calgary biofilm device, often referred to as the mbec system, as a high-throughput approach to the production and subsequent characterization of pleurotus ostreatus biofilms was assessed. the hydroxyapatite-coating of pegs was necessary to enable biofilm attachment, and the standardization of vegetative inocula ensured a uniform distribution of p. ostreatus biofilms, which is necessary for high-throughput evaluations of several antimicrobials and exposure conditions. scanning ...201323892744
[morphology and structural peculiarities of basidiomycetes pathogens].the materials of studies of morphology and structural peculiarities of viruses, fungi and bacteria, which affect basidiomycetes under biotechnology process and nature biocenosis conditions are given. the analysis of infection development in button mushroom (agaricus bisporus) (j.lge) imbach and in oyster mushroom (pleurotus ostreatus kumm.), which served as model objects in the experiments of various levels of complexity has been carried out. other kinds of edible and medicinal mushrooms, which ...201323866587
membrane cholesterol and sphingomyelin, and ostreolysin a are obligatory for pore-formation by a macpf/cdc-like pore-forming protein, pleurotolysin b.the mushroom pleurotus ostreatus has been reported to produce the hemolytic proteins ostreolysin (olya), pleurotolysin a (plya) and pleurotolysin b (plyb). the present study of the native and recombinant proteins dissects out their lipid-binding characteristics and their roles in lipid binding and membrane permeabilization. using lipid-binding studies, permeabilization of erythrocytes, large unilamellar vesicles of various lipid compositions, and electron microscopy, we show that olya, a plya ho ...201323806422
degradation of lindane and endosulfan by fungi, fungal and bacterial laccases.the ability of two white-rot fungi (trametes versicolor and pleurotus ostreatus) and one brown-rot fungus (gloeophyllum trabeum) to degrade two organochlorine insecticides, lindane and endosulfan, in liquid cultures was studied and dead fungal biomass was examined for adsorption of both insecticides from liquid medium. lindane and endosulfan were also treated with fungal laccase and bacterial protein cota, which has laccase activities. the amount of degraded lindane and endosulfan increased with ...201323736895
effect of light and atmosphere on the cultivation of the golden oyster culinary-medicinal mushroom, pleurotus citrinopileatus (higher basidiomycetes).with an aim to explore the productivity and quality of the fruiting body of culinary-medicinal golden oyster mushroom pleurotus citrinopileatus, the carbon dioxide (co₂) concentration of the ambient atmosphere was adjusted and a light-emitting diode panel was used to illuminate the colonized mycelium at different wavelengths. biological efficiency and yield were higher at co₂ levels of 0.05 and 0.1% than other tested co₂ levels, and the mature fruiting body showed the highest yellow value at a c ...201323510289
[white-rot fungi combinations impact on enzyme productions].in this study, the maximum laccase (lac) activities by single white-rot fungus and combining white-rot fungi were investigated. the optimal combinations of white-rot fungi were obtained by comparing the maximum lac activities and the time of achieving the maximum lac activity. the results showed that the synergy of white-rot fungi polyporellus picipes and pleurotus ostreatus improved the secretion of the laccase, with the enzyme production of 50.45 u x ml(-1), as well as the combination of white ...201323487950
induction of laccase activity in the white rot fungus pleurotus ostreatus using water polluted with wheat straw extracts.the purpose of this work was to explore the use of polluted water effluents from wheat straw using industries as inducers of lignocellulolytic enzymatic activities in cultures of white rot basidiomycetes. for this purpose, we studied the effect of a wheat straw water extract on the evolution of the laccase activity recovered from submerged cultures of pleurotus ostreatus made in different media and under various culture conditions. our results demonstrated an accumulative induction effect in all ...201323425584
pleurotus ostreatus biofilm-forming ability and ultrastructure are significantly influenced by growth medium and support type.to investigate the effect of support and growth medium (gm) on pleurotus ostreatus biofilm production, specific metabolic activity (sma) and ultrastructure.201323414514
in vitro antioxidant and antimicrobial activities of two edible mushroom mycelia obtained in the presence of different nitrogen sources.the antioxidant and antimicrobial properties of two edible mushrooms (pleurotus ostreatus pbs281009 and coprinus comatus m8102) were evaluated in this study. the mycelium ethanolic extract obtained in the presence of four different nitrogen sources was investigated with regard to their production of phenolic compounds, β-carotene, lycopene, and their antimicrobial and antioxidant properties. significant differences in phenol and β-carotene concentrations in mycelia grown in different nitrogen so ...201323289786
redundancy among manganese peroxidases in pleurotus ostreatus.manganese peroxidases (mnps) are key players in the ligninolytic system of white rot fungi. in pleurotus ostreatus (the oyster mushroom) these enzymes are encoded by a gene family comprising nine members, mnp1 to -9 (mnp genes). mn(2+) amendment to p. ostreatus cultures results in enhanced degradation of recalcitrant compounds (such as the azo dye orange ii) and lignin. in mn(2+)-amended glucose-peptone medium, mnp3, mnp4, and mnp9 were the most highly expressed mnp genes. after 7 days of incuba ...201323377936
sugarcane bagasse degradation and characterization of three white-rot fungi.in order to investigate the details of lignin biodegradation, the characteristics and process of sugarcane bagasse (scb) degradation by three lignin degrading fungi, phanerochaete chrysosporium pc2, lentinula edode le16 and pleurotus ostreatus po45, were studied. we found that the ligninolytic enzymes polyphenol oxidase (ppo) and manganese peroxidase (mnp) were produced first, and that the cellulolytic enzyme cmcase was produced subsequently. these three fungi were more efficient to degrade lign ...201323376835
transcriptional analysis of pleurotus ostreatus laccase genes.fungal laccases (p-diphenol:oxygen oxidoreductase; ec 1.10.3.2) are multi-copper-containing oxidases that catalyse the oxidation of a great variety of phenolic compounds and aromatic amines through simultaneous reduction of molecular oxygen to water. fungi generally produce several laccase isoenzymes encoded by complex multi-gene families. the pleurotus ostreatus genome encodes 11 putative laccase coding genes, and only six different laccase isoenzymes have been isolated and characterised so far ...201322395908
antioxidant properties of polysaccharides obtained by batch cultivation of pleurotus ostreatus mycelium.the antioxidant activity of the exopolysaccharides (eps) and intracellular polysaccharides (ips) obtained from the mycelia obtained by submerged culture of two edible pleurotus ostreatus strains, pqmz91109 and psi101109 in a batch bioreactor was determined. eps and ips showed significant antioxidant activities (in vitro) especially in the scavenging effect on the dpph and abts radicals, reducing power and chelating effect on ferrous ions. the tests proved the differences between the two mushroom ...201322784237
antihypercholesterolemic and antioxidative effects of an extract of the oyster mushroom, pleurotus ostreatus, and its major constituent, chrysin, in triton wr-1339-induced hypercholesterolemic rats.hypercholesterolemia and oxidative stress are known to accelerate coronary artery disease and progression of atherosclerotic lesions. in the present study, an attempt was made to evaluate the putative antihypercholesterolemic and antioxidative effects of an ethanolic extract of the oyster mushroom (pleurotus ostreatus) and chrysin, one of its major components, in hypercholesterolemic rats. hypercholesterolemia was induced in rats by a single intraperitoneal injection of triton wr-1339 (300 mg/kg ...201323104078
pilot study of bioaccumulation and distribution of cesium, potassium, sodium and calcium in king oyster mushroom (pleurotus eryngii) grown under controlled conditions.this pilot study presents preliminary results on interrelations between alkali and alkaline earth elements during their transfer to mycelium and fruitbodies of saprophytic fungi. the accumulation and distribution of four elements (cesium, potassium, sodium, and calcium) was evaluated in king oyster mushroom (pleurotus eryngii) cultivated under controlled conditions. elemental composition of caps, stipes, and the substrate was analyzed by atomic absorption/emission spectroscopy to evaluate discri ...201319260229
production and purification of statins from pleurotus ostreatus (basidiomycetes) strains.pleurotus ostreatus strains were cultured in liquid medium and on wheat straw. the yields of lovastatin were compared.201312622228
degradation of lignocellulose by pleurotus ostreatus in the presence of copper, manganese, lead and zinc.pleurotus ostreatus produces the cellulolytic and hemicellulolytic enzymes endo-1,4-beta-glucanase, exo-1,4-beta-glucanase, 1,4-beta-glucosidase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase, endo-1,4-beta-mannanase and 1,4-beta-mannosidase and ligninolytic enzymes mn-peroxidase and laccase during growth on wheat straw in the presence and absence of cu, mn, pb, and zn. this is the first report concerning endo-1,4-beta-mannanase in p. ostreatus. although the concentrations of trace metals in wheat ...201315921894
lovastatin production by pleurotus ostreatus: effects of the c:n ratio.the types of carbon source and nitrogen source used as well as the c:n ratio in the medium influenced lovastatin production by pleurotus ostreatus. the maximum value of the lovastatin yield was obtained in a medium that contained organic nitrogen.201316610224
mn2+ alters peroxidase profiles and lignin degradation by the white-rot fungus pleurotus ostreatus under different nutritional and growth conditions.the white-rot fungus pleurotus ostreatus produces two types of extracellular peroxidases: manganese-dependent peroxidase (mnp) and versatile peroxidase (vp). the effect of mn2+ on fungal growth, peroxidase activity profiles, and lignin degradation by p. ostreatus was studied in liquid culture and under solid-state fermentation conditions on perlite, the latter resembling the natural growth conditions of this fungus. the fungus was grown in either a defined asparagine-containing basidiomycete sel ...201312396142
mechanism and potential applications of bio-ligninolytic systems in a celss.a large amount of inedible plant material, generated as a result of plant growth in a controlled ecological life support system (celss), should be pretreated and converted into forms that can be recycled on earth as well as in space. the main portion of the inedible biomass is lignocellulosic material. enzymatic hydrolysis of this cellulose would provide sugars for many other uses by recycling carbon, hydrogen, oxygen, and nitrogen through formation of carbon dioxide, heat, and sugars, which are ...20139170251
ferulic acid as the inductor of messenger rna synthesis related to laccase formation in the wood rotting fungus pleurotus ostreatus. 20134206471
characteristic odorants from bailingu oyster mushroom (pleurotus eryngii var. tuoliensis) and summer oyster mushroom (pleurotus cystidiosus).in this study, the characteristic odorants of the volatile oils from pleurotus species (p. eryngii var. tuoliensis and p. cystidiosus) were extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (gc-ms), gas chromatography-olfactometry (gc-o), and aroma extract dilution analysis (aeda). a total of 52 and 54 components (p. eryngii var. tuoliensis and p. cystidiosus, respectively) were identified, representing about 98.8% and 85.1% of the volatile oils, respectively. t ...201424919476
a-wings: an integrated genome database for pleurocybella porrigens (angel's wing oyster mushroom, sugihiratake).the angel's wing oyster mushroom (pleurocybella porrigens, sugihiratake) is a well-known delicacy. however, its potential risk in acute encephalopathy was recently revealed by a food poisoning incident. to disclose the genes underlying the accident and provide mechanistic insight, we seek to develop an information infrastructure containing omics data. in our previous work, we sequenced the genome and transcriptome using next-generation sequencing techniques. the next step in achieving our goal i ...201425465051
cultivation of oyster mushroom pleurotus ostreatus on date-palm leaves mixed with other agro-wastes in saudi arabia.promoting the use of agricultural waste is one of the newly prepared water and environment friendly agriculture strategies in the kingdom of saudi arabia (ksa). the objective of this research was to study the efficiency of cultivating oyster mushroom (pleurotus ostreatus) on date palm wastes mixed with other agricultural wastes available in ksa. four agricultural wastes were mixed with date palm leaves at different ratios, with two supplements and three spawn rates were used. wheat straw mixed w ...201425473372
effect of ultraviolet-a (uv-a) and ultraviolet-c (uv-c) light on mechanical properties of oyster mushrooms during growth.this study investigated the effects of ultraviolet-a (uv-a) and ultraviolet-c (uv-c) light on the mechanical properties in oyster mushrooms during the growth. experiments were carried out with irradiation of the mushrooms with uv-a (365 nm) and uv-c (254 nm) light during growth. the exposure time ranged from 10 minutes to 60 minutes at intervals of 10 minutes and irradiation was done for three days. the samples for experimental studies were cut into cylindrical shapes of diameter 12.50 mm and th ...201425580117
anti-allergic effect of pleuran (β-glucan from pleurotus ostreatus) in children with recurrent respiratory tract infections.recurrent respiratory tract infections (rrtis) present a very important problem in paediatric praxis. as true immunodeficiencies are rare, one of the most important factors assumed to contribute to increased respiratory morbidity is atopy. several preparations of natural origin have been used for the prevention of rrtis, and some of the most effective immunomodulators are biologically active polysaccharides - e.g. ß-glucans. in our randomised, double-blind, placebo-controlled study, we investiga ...201423744488
determination of anti-inflammatory activities of standardised preparations of plant- and mushroom-based foods.chronic inflammatory processes contribute to the pathogenesis of many age-related diseases. in search of anti-inflammatory foods, we have systematically screened a variety of common dietary plants and mushrooms for their anti-inflammatory activity.201423653285
thiabendazole uptake in shimeji, king oyster, and oyster mushrooms and its persistence in sterile and nonsterile substrates.thiabendazole in the substrates incurred from spraying and premixing was translocated to the pileus, stipe, and volva of selected mushrooms. the spraying on the substrates resulted in higher residues of thiabendazole in all three mushrooms than the premixing treatment. for premixing, in the five substrates, half-lives of thiabendazole were found to be 13.6 days for shimeji, 10.0 days for king oyster, 13.7 days for oyster, 19.1 days for sterilized substrate, and 8.4 days for nonsterilized substra ...201424432721
pleurotus ostreatus, an edible mushroom, enhances glucose 6-phosphate dehydrogenase, ascorbate peroxidase and reduces xanthine dehydrogenase in major organs of aged rats.aging is now considered to be associated with an elevation in oxidative damage to macromolecules and enhanced levels of inflammation. therefore, inhibition of age-related oxidative stress by natural supplement is an important study.201424392756
ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability.the genome of pleurotus ostreatus, an important edible mushroom and a model ligninolytic organism of interest in lignocellulose biorefineries due to its ability to delignify agricultural wastes, was sequenced with the purpose of identifying and characterizing the enzymes responsible for lignin degradation.201424387130
purification and characterization of an extracellular laccase from solid-state culture of pleurotus ostreatus hp-1.a native isolate of pleurotus ostreatus hp-1 (genbank accession no. eu420068) was found to have an excellent laccase producing ability. the extracellular laccase was purified to electrophoretic homogeneity from copper sulphate induced solid-state fermentation medium by ammonium sulphate precipitation and ion-exchange chromatography. the enzyme was determined to be monomeric protein with an apparent molecular mass of 68,420 kda, and an isoelectric point (pi) of 3.5. the inductively coupled plasma ...201428324461
mycoremediation of benzo[a]pyrene by pleurotus ostreatus in the presence of heavy metals and mediators.benzo[a]pyrene is considered as a priority pollutant because of its carcinogenic, teratogenic and mutagenic effects. the highly recalcitrant nature of benzo[a]pyrene poses a major problem for its degradation. white-rot fungi such as pleurotus ostreatus can degrade benzo[a]pyrene by enzymes like laccase and manganese peroxidase. the present investigation was carried out to determine the extent of benzo[a]pyrene degradation by the po-3, a native isolate of p. ostreatus, in the presence of heavy me ...201428324451
engineering a fungal peroxidase that degrades lignin at very acidic ph.ligninolytic peroxidases are divided into three families: manganese peroxidases (mnps), lignin peroxidases (lips), and versatile peroxidases (vps). the latter two are able to degrade intact lignins, as shown using nonphenolic lignin model compounds, with vp oxidizing the widest range of recalcitrant substrates. one of the main limiting issues for the use of these two enzymes in lignocellulose biorefineries (for delignification and production of cellulose-based products or modification of industr ...201425788979
cloning, expression, and characterization of a milk-clotting aspartic protease gene (po-asp) from pleurotus ostreatus.an aspartic protease gene from pleurotus ostreatus (po-asp) had been cloned based on the 3' portion of cdna in our previous work. the po-asp cdna contained 1,324 nucleotides with an open reading frame (orf) of 1,212 bp encoding 403 amino acid residues. the putative amino acid sequence included a signal peptide, an activation peptide, two most possible n-glycosylation sites and two conserved catalytic active site. the mature polypeptide with 327 amino acid residues had a calculated molecular mass ...201424338250
dynamics of sterols and fatty acids during uv-b treatment of oyster mushroom.fruiting bodies of the oyster mushroom pleurotus ostreatus were illuminated with uv-b with a light intensity maximum at 310-320 nm and 11.5 w/m² for 60 min at 20 °c. changes of the sterol and fatty acid spectrum were quantified. the onset of ergocalciferol (vitamin d₂) formation was immediate in fruiting bodies illuminated from the lamella side, in sliced fruiting bodies, and in the stipes. saturation concentrations above 100 μg/g of dry matter were reached after 1h. at the same time, the concen ...201424295670
hydrophobin-coated plates as matrix-assisted laser desorption/ionization sample support for peptide/protein analysis.fungal hydrophobins are amphipathic self-assembling proteins. vmh2 hydrophobin, prepared from mycelial cultures of the basidiomycete fungus pleurotus ostreatus, spontaneously forms a stable and homogeneous layer on solid surfaces and is able to strongly absorb proteins even in their active forms. in this work, we have exploited the vmh2 self-assembled layer as a novel coating of a matrix-assisted laser desorption/ionization (maldi) steel sample-loading plate. mixtures of standard proteins, as we ...201424287080
essential role of the n- and c-terminals of laccase from pleurotus florida on the laccase activity and stability.poxa1b is the most thermostable laccase isoenzyme from pleurotus ostreatus. poxa1b is remarkably stable at alkaline ph (the t1/2 at ph 10 was 30 days), and its c-terminal affects its catalytic and stability properties. we cloned poxa1c from p. florida, which showed 99 % identity with poxa1b. poxa1c was functionally expressed in pichia pastoris. the functions of the n and c termini of poxa1c were investigated using site-directed mutagenesis. compared with poxa1c, the n-terminal r5v site effective ...201425161036
antioxidant properties of mushroom mycelia obtained by batch cultivation and tocopherol content affected by extraction procedures.the determination of the antioxidant potential of lyophilized mushroom mycelia from 5 strains of the species pleurotus ostreatus and coprinus comatus (obtained by submerged cultivation in batch system) was analyzed as ethanolic extracts by evaluating abts and the hydroxyl scavenging activity, frap method, the chelating capacity, the inhibition of human erythrocyte hemolysis, and the inhibition of xanthine oxidase activity. the main compounds present in all extracts were determined by hplc chroma ...201425110715
anti-tumor and macrophage activation induced by alkali-extracted polysaccharide from pleurotus ostreatus.pleurotus ostreatus is popularly consumed as traditional medicine and health food for enhancing immune function in china. polysaccharides from mushroom have been demonstrated to possess a wide range of health beneficial properties. this study was carried out to elucidate the immunomodulating effects and molecular mechanism involved in the in vivo and in vitro anti-tumor activities of alkali-extracted polysaccharide (wpop-n1) from the fruiting bodies of p. ostreatus. the results showed that wpop- ...201424942990
x-ray crystallographic structure of rnase po1 that exhibits anti-tumor activity.rnase po1 is a guanylic acid-specific ribonuclease member of the rnase t1 family from pleurotus ostreatus. we previously reported that rnase po1 inhibits the proliferation of human tumor cells, yet rnase t1 and other t1 family rnases are non-toxic. we determined the three-dimensional x-ray structure of rnase po1 and compared it with that of rnase t1. the catalytic sites are conserved. however, there are three disulfide bonds, one more than in rnase t1. one of the additional disulfide bond is in ...201424882409
comparative evaluation of polysaccharides isolated from astragalus, oyster mushroom, and yacon as inhibitors of α-glucosidase.the incidence of diabetes has increased considerably, and become the third serious chronic disease following cancer and cardiovascular diseases. though acarbose, metformin, and 1-deoxynojirimycin have good efficacy for clinical application as hypoglycemic drugs, their expensive costs and some degree of side effects have limited their clinical application. recently, increasing attention has concentrated on the polysaccharides from natural plant and animal sources for diabetes. in order to illustr ...201424863354
biodegradation of heptachlor and heptachlor epoxide-contaminated soils by white-rot fungal inocula.the ability of certain white-rot fungi (wrf) inocula to transform heptachlor and heptachlor epoxide and its application in artificially contaminated soil were investigated. fungal inoculum of pleurotus ostreatus eliminated approximately 89 % of heptachlor after 28 days of incubation, and chlordene was detected as the primary metabolite. the fungal inoculum of pleurotus ostreatus had the highest ability to degrade heptachlor epoxide; approximately 32 % were degraded after 28 days of incubation, a ...201424840358
fungal macpf-like proteins and aegerolysins: bi-component pore-forming proteins?proteins with membrane-attack complex/perforin (macpf) domains are found in almost all kingdoms of life, and they have a variety of biological roles, including defence and attack, organism development, and cell adhesion and signalling. the distribution of these proteins in fungi appears to be restricted to some pezizomycotina and basidiomycota species only, in correlation with another group of proteins with unknown biological function, known as aegerolysins. these two protein groups coincide in ...201424798017
evaluation of biomass of some invasive weed species as substrate for oyster mushroom (pleurotus spp.) cultivation.this study assessed the bioconversion of agriculture wastes like invasive weeds species (lantana camara, prosopis juliflora, parthenium hysterophorus) as a substrate for oyster mushroom (pleurotus species) cultivation together with wheat straw as a control. the experiment was laid out in factorial combination of substrates and three edible oyster mushroom species in a completely randomized design (crd) with three replications. pleurotus ostreatus gave significantly (p < 0.01) total yield of 840 ...201424783804
isolation, culture optimization and physico-chemical characterization of laccase enzyme from pleurotus fossulatus.pleurotus fossulatus (cooke) sace is member of oyster mushroom can produced extracellular laccase (benzenediol: oxygen oxidoreductase; ec 1.10.3.2) in submerged fermentation. to analyze the optimum production for laccase p. fossulatus was cultured both in stationary and shaking condition in different media. partial purification of laccase was done after 0-80% ammonium sulphate precipitation, followed by deae (diethylaminoethyl) sephadex (a-50) anion exchange chromatography. potato-sucrose pepton ...201424783799
permeability characteristics of cell-membrane pores induced by ostreolysin a/pleurotolysin b, binary pore-forming proteins from the oyster mushroom.proteins from the oyster mushroom, 15 kda ostreolysin a (olya), and 59 kda pleurotolysin b (plyb) with a membrane attack complex/perforin (macpf) domain, damage cell membranes as a binary cytolytic pore-forming complex. measurements of single-channel conductance and transmembrane macroscopic current reveal that olya/plyb form non-selective ion-conducting pores with broad, skewed conductance distributions in n18 neuroblastoma and cho-k1 cell membranes. polyethylene-glycol 8000 (hydrodynamic radiu ...201424211835
determination of statins by gas chromatography - ei/mrm - tandem mass spectrometry: fermentation of pine samples with pleurotus ostreatus.statins were separated and quantified with gas chromatography-mass spectrometry (gc-ei-ms/ms) using total ion monitoring (tic) and multiple reactions monitoring (mrm). the mrm method in statins determination has a novelty value, since there are no previous studies on their simultaneous analysis in environmental or plant samples. the method development and optimization was challenging due to the physicochemical similarities of the silylated lovastatin, simvastatin, pravastatin, fluvastatin, and a ...201424607418
molecular divergence and species delimitation of the cultivated oyster mushrooms: integration of igs1 and its.identification of edible mushrooms particularly pleurotus genus has been restricted due to various obstacles. the present study attempted to use the combination of two variable regions of igs1 and its for classifying the economically cultivated pleurotus species. integration of the two regions proved a high ability that not only could clearly distinguish the species but also served sufficient intraspecies variation. phylogenetic tree (igs1+its) showed seven distinct clades, each clade belonging ...201424587752
ligninolytic peroxidase gene expression by pleurotus ostreatus: differential regulation in lignocellulose medium and effect of temperature and ph.pleurotus ostreatus is an important edible mushroom and a model lignin degrading organism, whose genome contains nine genes of ligninolytic peroxidases, characteristic of white-rot fungi. these genes encode six manganese peroxidase (mnp) and three versatile peroxidase (vp) isoenzymes. using liquid chromatography coupled to tandem mass spectrometry, secretion of four of these peroxidase isoenzymes (vp1, vp2, mnp2 and mnp6) was confirmed when p. ostreatus grows in a lignocellulose medium at 25°c ( ...201424560615
the perforin pore facilitates the delivery of cationic cargos.cytotoxic lymphocytes eliminate virally infected or neoplastic cells through the action of cytotoxic proteases (granzymes). the pore-forming protein perforin is essential for delivery of granzymes into the cytoplasm of target cells; however the mechanism of this delivery is incompletely understood. perforin contains a membrane attack complex/perforin (macpf) domain and oligomerizes to form an aqueous pore in the plasma membrane; therefore the simplest (and best supported) model suggests that gra ...201424558045
decontamination of a municipal landfill leachate from endocrine disruptors using a combined sorption/bioremoval approach.sorption and biodegradation are the main mechanisms for the removal of endocrine disruptor compounds (eds) from both solid and liquid matrices. there are recent evidences about the capacity of white-rot fungi to decontaminate water systems from phenolic eds by means of their ligninolytic enzymes. most of the available studies report the removal of eds by biodegradation or adsorption separately. this study assessed the simultaneous removal of five eds—the xenoestrogens bisphenol a (bpa), ethynile ...201424122163
inactivation of a pleurotus ostreatus versatile peroxidase-encoding gene (mnp2) results in reduced lignin degradation.lignin biodegradation by white-rot fungi is pivotal to the earth's carbon cycle. manganese peroxidases (mnps), the most common extracellular ligninolytic peroxidases produced by white-rot fungi, are considered key in ligninolysis. pleurotus ostreatus, the oyster mushroom, is a preferential lignin degrader occupying niches rich in lignocellulose such as decaying trees. here, we provide direct, genetically based proof for the functional significance of mnp to p. ostreatus ligninolytic capacity und ...201424119015
changes in chemical composition and digestibility of three maize stover components digested by white-rot fungi.maize stover (total stem and leaves) is not considered a ruminant feed of high nutritive value. therefore, an improvement in its digestibility may increase the viability of total forage maize production systems in marginal growth regions. the objective of this study was to describe the changes in chemical composition during the storage of contrasting components of maize stover (leaf, upper stem and lower stem) treated with either of two lignin degrading white-rot fungi (wrf; pleurotus ostreatus, ...201424112093
polluting macrophytes colombian lake fúquene used as substrate by edible fungus pleurotus ostreatus.invasive aquatic plants from lake fúquene (cundinamarca, colombia), water hyacinth (eichhornia crassipes c. mart.) and brazilian elodea (egeria densa planch.) have been removed mechanically from the lake and can be used for edible mushrooms production. the growth of the oyster mushroom (pleurotus ostreatus) on these aquatic macrophytes was investigated in order to evaluate the possible use of fruiting bodies and spent biomass in food production for human and animal nutrition, respectively. treat ...201423900906
biodegradation of wheat straw by pleurotus ostreatus.wheat straw pretreated with chemicals as well as hot water was subjected to degradation by edible mushroom pleurotus ostreatus. lignin, cellulose and hemicelluloses component of both chemically as well as hot water treated wheat straw was degraded by the fungus and in turn the edible and nutritious fruiting body of the mushroom was produced. biodegradation of wheat straw in terms of loss of lignin, cellulose and hemicellulose showed positive correlation with cellulases, xylanase, laccase and pol ...201425535709
bioremediation of vegetable and agrowastes by pleurotus ostreatus: a novel strategy to produce edible mushroom with enhanced yield and nutrition.pleurotus ostreatus was grown on paddy straw as well as other vegetable and agricultural wastes i.e. pea pod shell, cauliflower leaves, radish leaves and brassica straw in various combinations with paddy straw. the mushroom did not grow on the vegetable wastes separately. the cumulative yield and biological efficiency of the edible oyster mushroom p. ostreatus grown on substrate containing paddy straw in various combinations with different vegetable wastes i.e. 20% and 30 % vegetable wastes mixe ...201425535705
pleurotus ostreatus opposes mitochondrial dysfunction and oxidative stress in acetaminophen-induced hepato-renal injury.acetaminophen (apap)-induced toxicity is a predominant cause of acute hepatic and renal failure. in both humans and rodents toxicity begins with a reactive metabolite that binds to proteins. this leads to mitochondrial dysfunction and nuclear dna fragmentation resulting in necrotic cell death. pleurotus ostreatus (an edible oyster mushroom) is well recognized as a flavourful food, as well as a medicinal supplement. in the present study, we evaluated the role of pleurotus ostreatus in the protect ...201425510860
highly expressed captured genes and cross-kingdom domains present in helitrons create novel diversity in pleurotus ostreatus and other fungi.helitrons are class-ii eukaryotic transposons that transpose via a rolling circle mechanism. due to their ability to capture and mobilize gene fragments, they play an important role in the evolution of their host genomes. we have used a bioinformatics approach for the identification of helitrons in two pleurotus ostreatus genomes using de novo detection and homology-based searching. we have analyzed the presence of helitron-captured genes as well as the expansion of helitron-specific helicases i ...201425480150
co-pyrolysis behaviour and kinetic of two typical solid wastes in china and characterisation of activated carbon prepared from pyrolytic char.this is the first study on the co-pyrolysis of spent substrate of pleurotus ostreatus and coal tar pitch, and the activated carbon prepared from the pyrolytic char. thermogravimetry (tg) analysis was carried out taking spent substrate, coal tar pitch and spent substrate-coal tar pitch mixture. the activation energies of pyrolysis reactions were obtained via the flynn-wall-ozawa and kissinger-akahira-sunose methods. the kinetic models were determined by the master-plots method. the activated carb ...201425378256
mutagenesis of the novel hericium erinaceus ribonuclease, rnase he1, reveals critical responsible residues for enzyme stability and activity.here, we determined the sequence of a cdna encoding a guanylic acid-specific ribonuclease (rnase he1) from hericium erinaceus that exhibits high sequence identity (59%) with rnase po1, an enzyme with anti-cancer activity and which is found in pleurotus ostreatus. rnase he1 and rnase po1 have similar structures and heat stabilities; hence, rnase he1 may also have potential as an anti-cancer agent. therefore, we initiated structure-function studies to further characterize the enzyme. based on the ...201425366489
characterization of an anti-gout xanthine oxidase inhibitor from pleurotus ostreatus.we selected pleurotus ostreatus from among several edible mushrooms because it has high anti-gout xanthine oxidase (xod) inhibitory activity. the maximal amount of xod inhibitor was extracted when the pleurotus ostreatus fruiting body was treated with distilled water at 40℃ for 48 hr. the xod inhibitor thus obtained was purified by sephadex g-50 gel permeation chromatography, ultrafiltration, c18 solid phase extraction chromatography and reverse-phase high-performance liquid chromatography with ...201425346610
telomerase inhibitory effects of medicinal mushrooms and lichens, and their anticancer activity.telomerase has been widely accepted as a cancer marker and a promising therapeutic target for novel anticancer drugs. the aim of this study was to investigate the in vitro telomerase inhibitory effects of mushrooms and their anticancer properties. the inhibitory effects of mushrooms and lichens against telomerase activity of hl-60 cells were systematically assessed using polymerase chain reaction based on assay of telomeric repeat amplification protocol. telomerase inhibitory samples were furthe ...201424940901
immobilization of a pleurotus ostreatus laccase mixture on perlite and its application to dye decolourisation.in the present study, a crude laccase preparation from pleurotus ostreatus was successfully immobilized on perlite, a cheap porous silica material, and tested for remazol brilliant blue r (rbbr) decolourisation in a fluidized bed recycle reactor. results showed that rbbr decolourisation is mainly due to enzyme action despite the occurrence of dye adsorption-related enzyme inhibition. fine tuning of immobilization conditions allowed balancing the immobilization yield and the resulting rate of dec ...201424895564
evaluation of the efficacy of fermented by-product of mushroom, pleurotus ostreatus, as a fish meal replacer in juvenile amur catfish, silurus asotus: effects on growth, serological characteristics and immune responses.the present experiment was conducted to evaluate the efficacy of dietary fermented by-product of mushroom, pleurotus ostreatus, (fbpm) as a fish meal (fm) replacer in juvenile amur catfish, silurus asotus. a total number of 225 fish averaging 5.7±0.1 g (mean±standard deviation) were fed one of the five experimental diets formulated to replace fm with fbpm at 0%, 5%, 10%, 20%, and 30% (fbpm0, fbpm5, fbpm10, fbpm20, and fbpm30, respectively). at the end of eight weeks of the experiment, average we ...201425178300
characterization of interspecific hybrid dikaryons of the oyster mushrooms, pleurotus florida pau-5 and p. sajor-caju pau-3 (higher basidiomycetes) from india.five pleurotus hybrid dikaryons, developed through cross-breeding of p. florida pau-5 (pf-5) and p. sajor-caju pau-3 (psc-3) were characterized with respect to textural properties, color, and enzymatic and genetic variability. texture profile revealed significant differences in springiness, resilience, cohesiveness, and chewiness between all hybrids compared to the parents. among the hybrid cultures, maximum whiteness was reported in hybrid 37, whereas hybrid 8 had minimum whiteness. three hybri ...201425271864
hepato- and nephroprotective activities of a nigerian local king tuber oyster mushroom, pleurotus tuberregium (higher basidiomycetes), in chemically induced organ toxicities in rats.chronic kidney disease, end-stage renal failure, and liver diseases are increasing worldwide and constitute a huge burden on health care costs, with attendant high morbidity and debility. despite advances in modern medicine, there are still no licensed drugs that satisfactorily restore lost kidney or hepatic functions. in this study the chemoprotective effects of the hot aqueous extract of a local edible oyster mushroom, pleurotus tuberregium (aptr), was evaluated in experimental liver and kidne ...201425271859
impact of laccase enzyme inducers on solid waste compost maturity and stability.the influence of inducers of laccase produced by pleurotus ostreatus-p1 on the physicochemical properties of solid waste compost was investigated. in order to examine the degree of humification and time period for maturity and stability of compost, three different treatments (t: laccase; t1: laccase+cuso4; and t2: laccase+cuso4+cacl2) were inoculated on solid waste in the initial stage of the composting process. during the composting process, the carbon nitrogen ratio (c/n), nitrate ([formula: s ...201425244141
antitumor activity of pleurotus ostreatus polysaccharide fractions on ehrlich tumor and sarcoma 180.the medicinal properties of fungi of the genus pleurotus have attracted great interest due to their therapeutic properties. polysaccharides synthesized by pleurotus, including the β-glucans are considered the main responsible for its therapeutic properties. this study aimed to evaluate the efficacy of polysaccharidic fractions extracted from mycelial biomass of pleurotus ostreatus dsm 1833 in inhibiting the development of ehrlich tumor (et) and sarcoma 180 (s-180). fc, fi and fii fractions provi ...201424768967
mn²⁺-deficiency reveals a key role for the pleurotus ostreatus versatile peroxidase (vp4) in oxidation of aromatic compounds.the manganese peroxidase gene family (mnps) is a part of the ligninolytic system of pleurotus ostreatus. this gene family is comprised of nine members, mnp1-9, encoding short manganese peroxidases (short-mnps) or versatile peroxidases (vps). we show that unlike in mn(2+)-amended glucose-peptone (gp) medium, where redundancy among mnps was reported, in mn(2+)-deficient gp medium mnp4 [encoding versatile peroxidase isoenzyme 4 (vp4)] has a key and nonredundant function. the abundance of mnps trans ...201424737058
statistical optimization of ultraviolet irradiate conditions for vitamin d₂ synthesis in oyster mushrooms (pleurotus ostreatus) using response surface methodology.response surface methodology (rsm) was used to determine the optimum vitamin d2 synthesis conditions in oyster mushrooms (pleurotus ostreatus). ultraviolet b (uv-b) was selected as the most efficient irradiation source for the preliminary experiment, in addition to the levels of three independent variables, which included ambient temperature (25-45°c), exposure time (40-120 min), and irradiation intensity (0.6-1.2 w/m2). the statistical analysis indicated that, for the range which was studied, i ...201424736742
effects of uv-b radiation levels on concentrations of phytosterols, ergothioneine, and polyphenolic compounds in mushroom powders used as dietary supplements.compositional changes of powder dietary supplements made from mushrooms exposed to different levels of uv-b irradiation were evaluated for the bioactive naturally occurring mushroom antioxidant, ergothioneine; other natural polyphenolic compounds, e.g., flavonoids, lignans, etc.; and selected phytosterols. four types of mushroom powder consisting of white, brown (agaricus bisporus), oyster (pleurotus ostreatus), and shiitake (lentinula edodes) mushrooms from three different treatment groups (con ...201424628700
biosorption of malachite green from aqueous solutions by pleurotus ostreatus using taguchi method.dyes released into the environment have been posing a serious threat to natural ecosystems and aquatic life due to presence of heat, light, chemical and other exposures stable. in this study, the pleurotus ostreatus (a macro-fungus) was used as a new biosorbent to study the biosorption of hazardous malachite green (mg) from aqueous solutions. the effective disposal of p. ostreatus is a meaningful work for environmental protection and maximum utilization of agricultural residues.the operational p ...201424620852
profile of natural redox mediators production of laccase-producing fungus pleurotus ostreatus.polycyclic aromatic hydrocarbons (pahs) are highly toxic organic pollutants which are abundant and environmentally widespread. anthracene is a simple pah that can be oxidized by laccases, copper-containing oxidase enzymes, produced by some plants, fungi, and bacteria. in this work, the extracellular culture fluid (cf) of laccase-producing fungus pleurotus ostreatus was separated to crude laccase (cl) and aqueous ultrafiltrate (au) fractions. the rate of anthracene oxidation by cf was 68.7 % whil ...201425108623
overproduction of laccase by the white-rot fungus pleurotus ostreatus using apple pomace as inducer.laccase activity of pleurotus ostreatus is significantly increased by the addition of apple pomace. among various conditions, the best concentration of apple pomace and cultivation time for the production of laccase by p. ostreatus was 2.5% and 9 days, respectively. reverse transcription polymerase chain reaction analyses of laccase isoenzyme genes, including pox1, pox3, pox4, poxc, poxa3, and poxa1b, revealed a clear effect of apple pomace on transcription induction. our findings reveal that th ...201425071391
high-level expression of a manganese superoxide dismutase (pomn-sod) from pleurotus ostreatus in pichia pastoris.the full-length cdna of pleurotus ostreatus superoxide dismutase (pomn-sod) was cloned and successfully expressed by using the ppic9k vector under the control of alcohol oxidase 1 promoter with a secretion signal peptide (α-factor) in pichia pastoris. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and western blotting demonstrated that recombinant pomn-sod, a 21.8 kda protein, was secreted into the culture medium. nondenaturing page experiments confirmed that recombinant po ...201425059984
differential response of oyster shell powder on enzyme profile and nutritional value of oyster mushroom pleurotus florida pf05.oyster mushroom pleurotus florida was cultivated on different combinations of wheat straw (ws) as basal substrate and oyster shell powder (osp) supplement. the osp supplementation considerably responded to different cultivation phases. the mycelium grew fast and showed rapid growth rate (8.91 mmd(-1)) in ws + osp (97 + 3) combination while ws + osp (92 + 8) showed maximum laccase (3.133 u/g) and mn peroxidase (mnp) activities (0.091 u/g). the climax level of laccase (5.433 u/g) and mnp (0.097 u/ ...201425054140
water-soluble polysaccharides from pleurotus ostreatus var. florida mycelial biomass.pleurotus ostreatus var. florida known as hiratake has a high nutritional value, presents medicinal and nutraceutical properties and it is one of the consumed mushrooms in brazil. thus, the aim of this study was to characterize the chemical structure of polysaccharides found in mycelial biomass produced by submerged culture of p. ostreatus var. florida in order to compare with those found in p. ostreatus var. florida fruit bodies. aqueous and alkali extracts obtained from mycelial biomass were p ...201425008131
potential of pleurotus ostreatus mycelium for selenium absorption.the aim of this study was to evaluate the effect of high selenium (se) concentrations on morphophysiological and ultrastructural properties of pleurotus ostreatus. mycelium growth was good in media enriched with 5.0, 10.0, and 20.0 mg l(-1) of se, concentration of 500.0 mg l(-1) strongly inhibited growth, and 1000.0 mg l(-1) was the minimum inhibitory concentration. contrary to thin-walled, hyaline, branched, and anastomized hyphae with clamp-connections in the control, at se concentrations of 1 ...201425003145
high removal of chemical and biochemical oxygen demand from tequila vinasses by using physicochemical and biological methods.the goal of this research is to find a more effective treatment for tequila vinasses (tvs) with potential industrial application in order to comply with the mexican environmental regulations. tvs are characterized by their high content of solids, high values of biochemical oxygen demand (bods), chemical oxygen demand (cod), low ph and intense colour; thus, disposal of untreated tvs severely impacts the environment. physicochemical and biological treatments, and a combination of both, were probed ...201424956770
development of an improved variant of gh51 α-l-arabinofuranosidase from pleurotus ostreatus by directed evolution.in this study, the α-l-arabinofuranosidase from pleurotus ostreatus was subjected to directed evolution by expressing a library of around 7000 randomly mutated variants by error prone polymerase chain reaction. high-throughput screening of the library for the most active variants was performed by assaying activity towards p-nitrophenyl α-l-arabinofuranoside, and a variant with higher activity than the wild type was selected, purified and characterised. it exhibited a kcat of 7.3 ×1 0¹ ± 0.3 min⁻ ...201424565609
toward an understanding of the effects of agitation and aeration on growth and laccases production by pleurotus ostreatus.mycelial growth and laccase production by pleurotus ostreatus cp50 cultured in a 10-l mechanically agitated bioreactor were assessed through a 2(3) factorial experimental design. the main effects and interactions of three factors (agitation, aeration and copper induction) over five responses (μ, αlacc, βlacc, maximal volumetric laccase activity and maximal biomass concentration) were analyzed. p. ostreatus growth was significantly improved when culturing was conducted with high agitation (5.9kw/ ...201424572371
microsatellites in the genome of the edible mushroom, volvariella volvacea.using bioinformatics software and database, we have characterized the microsatellite pattern in the v. volvacea genome and compared it with microsatellite patterns found in the genomes of four other edible fungi: coprinopsis cinerea, schizophyllum commune, agaricus bisporus, and pleurotus ostreatus. a total of 1346 microsatellites have been identified, with mono-nucleotides being the most frequent motif. the relative abundance of microsatellites was lower in coding regions with 21 no./mb. howeve ...201424575404
biosynthesis and characterization of silver nanoparticles produced by pleurotus ostreatus and their anticandidal and anticancer activities.the biosynthesis of nanoparticles has received increasing interest because of the growing need to develop safe, cost-effective and environmentally friendly technologies for the synthesis of nano-materials. in this study, silver nanoparticles (agnps) were synthesized using a reduction of aqueous ag(+) ions with culture supernatant from pleurotus ostreatus. the bioreduction of agnps was monitored by ultra violet-visible spectroscopy and the obtained agnps were characterized by transmission electro ...201425053172
[codon usage bias in the straw mushroom volvariella volvacea].we analyzed the whole genome coding sequence of volvariella volvacea to study the pattern utilization of codons by codon w 1.4.2. as results, 24 optimal codons were identified. moreover, the frequency of codons usage was calculated by cusp program. we compared the frequency of codons usage of v. volvacea with other organisms including 6 modal value species (homo sapiens, saccharomys cerevisiae, arabidopsis thalian, mus musculus, danio rerio and drosophila melanogaster) and 4 edible fungi (coprin ...201425720157
water recycle as a must: decolorization of textile wastewaters by plant-associated fungi.textile dye effluents are among the most problematic pollutants because of their toxicity on several organisms and ecosystems. low cost and ecocompatible bioremediation processes offer a promising alternative to the conventional and aspecific physico-chemical procedures adopted so far. here, microorganisms resident on three real textile dyeing effluent were isolated, characterized, and tested for their decolorizing performances. although able to survive on these real textile-dyeing wastewaters, ...201423553673
effective mutations in a high redox potential laccase from pleurotus ostreatus.since the first report on a laccase, there has been a notable development in the interest towards this class of enzymes, highlighted from the number of scientific papers and patents about them. at the same time, interest in exploiting laccases-mainly high redox potential-for various functions has been growing exponentially over the last 10 years. despite decades of work, the molecular determinants of the redox potential are far to be fully understood. for this reason, interest in tuning laccase ...201424463760
antifungal activity of cinnamic acid derivatives involves inhibition of benzoate 4-hydroxylase (cyp53).cyp53a15, from the sorghum pathogen cochliobolus lunatus, is involved in detoxification of benzoate, a key intermediate in aromatic compound metabolism in fungi. because this enzyme is unique to fungi, it is a promising drug target in fungal pathogens of other eukaryotes.201424314266
aflatoxin detoxification by manganese peroxidase purified from pleurotus ostreatus.manganese peroxidase (mnp) was produced from white rot edible mushroom pleurotus ostreatus on the culture filtrate. the enzyme was purified to homogeneity using (nh4)2so4 precipitation, deae-sepharose and sephadex g-100 column chromatography. the final enzyme activity achieved 81 u ml(-1), specific activity 78 u mg(-1) with purification fold of 130 and recovery 1.2% of the crude enzyme. sds-page indicated that the pure enzyme have a molecular mass of approximately 42 kda. the optimum ph was betw ...201424948923
screening of indigenously isolated fungi for lovastatin production and its in vivo evaluation.seven indigenously isolated fungal strains (aspergillus niger, aspergillus flavus, aspergillus terreus, penicillium citrinum, penicillium notatum, pleurotus ostreatus and trichoderma viradae) were tested for their potential to produce cholesterol lowering drug lovastatin by using different agro-industrial wastes (corn cobs, corn stover, banana stalk, wheat straw, wheat bran, bagasse) in submerged as well as solid state fermentation. aspergillus terreus showed maximum production of 18.74 mg/100 m ...201424894549
characterisation of potential antidiabetic-related proteins from pleurotus pulmonarius (fr.) quél. (grey oyster mushroom) by maldi-tof/tof mass spectrometry.pleurotus pulmonarius has been reported to have a potent remedial effect on diabetic property and considered to be an alternative for type 2 diabetes mellitus treatment. this study aimed to investigate the antidiabetic properties of ammonium sulphate precipitated protein fractions from p. pulmonarius basidiocarps. preliminary results demonstrated that 30% (nh4)2so4 precipitated fraction (f30) inhibited saccharomyces cerevisiae α-glucosidase activity (24.18%), and 100% (nh4)2so4 precipitated frac ...201425243114
medicinal and antimicrobial role of the oyster culinary-medicinal mushroom pleurotus ostreatus (higher basidiomycetes) cultivated on banana agrowastes in india.oyster mushrooms, species of the genus pleurotus, are recognized for producing secondary metabolites with important medicinal properties. investigations were carried out to evaluate the antioxidative and antimicrobial properties of the edible mushroom pleurotus ostreatus (mtcc142) extracts cultivated on banana agrowastes. ethanolic extracts showed antimicrobial activities against gram-positive and gram-negative bacteria, and their in vitro antifungal activities against all fungi tested revealed ...201424941164
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