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alteration of porcine serum albumin levels in pork meat by marination in kiwi or pineapple juice and subsequent pan broiling.this study was conducted to evaluate the changes in porcine serum albumin (psa), a major allergen, which occur when raw pork ham is marinated with kiwi or pineapple juice, and/or when the ham is pan broiled at 300℃ for 4 min after marination. in this study, raw pork ham was soaked for 4 h or 8 h in marinades containing commercial marinating sauce only, commercial marinating sauce and 7% kiwi juice, or commercial marinating sauce and 7% pineapple juice. when the meat was marinated and then pan-br ...201426761177
influence of harvest maturity and fruit logistics on pineapple (ananas comosus [l.] merr.) volatiles assessed by headspace solid phase microextraction and gas chromatography-mass spectrometry (hs-spme-gc/ms).profiling of volatiles from pineapple fruits was performed at four ripening stages using headspace solid-phase microextraction and gas chromatography-mass spectrometry (hs-spme-gc/ms). in total, 142 volatiles were detected, of which 132 were identified. multivariate data analysis was carried out to assess the effect of post-harvest storage on volatiles composition of green-ripe sea-freighted pineapple in comparison to air-freighted fruits harvested at full maturity. the latter fruits were charac ...201424360466
validation of a gc-ms method for the estimation of dithiocarbamate fungicide residues and safety evaluation of mancozeb in fruits and vegetables.a sensitive and rugged residue analysis method was validated for the estimation of dithiocarbamate fungicides in a variety of fruit and vegetable matrices. the sample preparation method involved reaction of dithiocarbamates with tin(ii) chloride in aqueous hcl. the cs2 produced was absorbed into an isooctane layer and estimated by gc-ms selected ion monitoring. limit of quantification (loq) was ⩽40μgkg(-1) for grape, green chilli, tomato, potato, brinjal, pineapple and chayote and the recoveries ...201424360436
prospective double-blind clinical trial evaluating the effectiveness of bromelain in the third molar extraction postoperative period.to evaluate the anti-inflammatory and analgesic effect of bromelain (pineapple extract) administered orally in the postoperative after extraction of impacted lower molars.201424316697
surface plasmon resonance optical sensor and antibacterial activities of biosynthesized silver nanoparticles.silver nanoparticles were prepared using aqueous fruit extract of ananas comosus as reducing agent. these silver nanoparticles showed surface plasmon peak at 439 nm. they were monodispersed and spherical in shape with an average particle size of 10 nm. the crystallinity of these nanoparticles was evident from clear lattice fringes in the hrtem images and bright circular spots in the saed pattern. the antibacterial activities of prepared nanoparticles were found to be size-dependent, the smaller ...201424291437
a superoxide dismutase purified from the roots from stemona tuberosa.proteins from the fresh roots of stemona tuberosa (stemonaceae) were extracted into 20 mm phosphate buffer, ph 7.2/0.1 m nacl, precipitated with 90% saturation ammonium sulfate, and enriched by diethylaminoethanol (deae) cellulose. the protein eluted as a single main peak from the unbound fractions (st-1), and appeared as a single band with superoxide dismutase (sod) activity after native polyacrylamide gel electrophoresis (page) resolution and zymogram development. st-1 was classified as sod du ...201424279794
sequential multi-layered hemostatic sutures for stepwise removal of arteriovenous malformations: the pineapple technique. 201424268693
assessment of fruit juice authenticity using uplc-qtof ms: a metabolomics approach.in recent years, with the growing complexity of global food supply chains and trade, food fraud, including adulteration of high value foods with cheaper substitutes, has become an increasingly important issue. a metabolomics approach can be applied to discover biomarkers that can be used to trace food adulteration. a study was undertaken to discover novel, potential biomarkers for the rapid detection of the adulteration of fruit juices with cheaper alternatives. pineapple, orange, grapefruit, ap ...201424262519
improving phosphorus availability in an acid soil using organic amendments produced from agroindustrial wastes.in acid soils, soluble inorganic phosphorus is fixed by aluminium and iron. to overcome this problem, acid soils are limed to fix aluminium and iron but this practice is not economical. the practice is also not environmentally friendly. this study was conducted to improve phosphorus availability using organic amendments (biochar and compost produced from chicken litter and pineapple leaves, resp.) to fix aluminium and iron instead of phosphorus. amending soil with biochar or compost or a mixture ...201425032229
therapeutic efficacy of bromelain in impacted third molar surgery: a randomized controlled clinical study.bromelain is the name given to a family of proteolytic enzymes obtained from ananas comosus, the pineapple plant. it is considered a potent anti-inflammatory and antiedematous substance. surgery of impacted third molars in the outpatient setting is one of the procedures most often associated with postoperative pain and swelling. the aim of this study was to evaluate the efficacy of bromelain in reducing postoperative pain and swelling.201425019111
collection and chemical composition of phloem sap from citrus sinensis l. osbeck (sweet orange).through utilizing the nutrient-rich phloem sap, sap feeding insects such as psyllids, leafhoppers, and aphids can transmit many phloem-restricted pathogens. on the other hand, multiplication of phloem-limited, uncultivated bacteria such as candidatus liberibacter asiaticus (clas) inside the phloem of citrus indicates that the sap contains all the essential nutrients needed for the pathogen growth. the phloem sap composition of many plants has been studied; however, to our knowledge, there is no ...201425014027
drinking pineapple juice for undigested food in stomach. 201425003688
impact of mixtures of different fresh-cut fruits on respiration and ethylene production rates.packaging and storage of fresh-cut fruits and vegetables are a challenging task, since fresh produce continue to respire and senesce after harvest and processing accelerates the physiological processes. the response on respiration and ethylene production rates of fresh produce to changes in o2 and co2 concentrations and temperature has been extensively studied for whole fruits but literature is limited on processed and mixed fresh-cut fruits. this study aimed to investigate the effects of mixing ...201424984574
draft genome sequence of kozakia baliensis sr-745, the first sequenced kozakia strain from the family acetobacteraceae.kozakia baliensis belongs to the family acetobacteraceae and was described for the first time in 2002. these acetic acid bacteria are able to produce acetic acid from various carbon sources and 2- and 5-keto-d-gluconate from glucose. the novel k. baliensis strain sr-745 was isolated from a pineapple fruit bought in a german supermarket. the strain produces large amounts of organic acids when grown on glucose-containing medium and accepts also glycerol, fructose, mannitol, and sucrose as a c sour ...201424970826
bromelain: an overview of industrial application and purification strategies.this review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approache ...201424965557
physico-chemical properties, antioxidant activity and mineral contents of pineapple genotypes grown in china.the fruit physico-chemical properties, antioxidant activity and mineral contents of 26 pineapple [ananas comosus (l.) merr.] genotypes grown in china were measured. the results showed great quantitative differences in the composition of these pineapple genotypes. sucrose was the dominant sugar in all 26 genotypes, while citric acid was the principal organic acid. potassium, calcium and magnesium were the major mineral constituents. the ascorbic acid (asa) content ranged from 5.08 to 33.57 mg/100 ...201424959679
a study on ultraviolet protection of 100% cotton knitted fabric: effect of fabric parameters.the effect of fabric parameters such as weight, thickness, and stitch density on the ultraviolet (uv) protection of knitted fabrics was studied. different knitting structures such as plain, pineapple, lacoste, and other combinations of different knitting stitches of knit, tuck, and miss as well as half milano, full milano, half cardigan, full cardigan, 1 × 1 rib, and interlock were prepared. experimental results revealed that weight was the most important factor that affected uv protection while ...201424955409
the prevalence of the oral allergy syndrome and pollen-food syndrome in an atopic paediatric population in south-west sydney.oral allergy syndrome (oas) and pollen-food syndrome (pfs) are regarded as uncommon manifestations of paediatric atopic disease. however, little australian data exist. the aim of this study was to examine the prevalence of oas in an atopic, paediatric population.201424953857
drivers of conservation and utilization of pineapple genetic resources in benin.valuation of farmer knowledge has been seen as a route to promote sustainable use of plant genetic resources. in pineapple production systems in benin, inadequate knowledge of cultivation practices can lead to a number of inconveniences including abandon of some varieties and cultivars. to understand how farmer's knowledge and cultivation practices impact the sustainable utilization of pineapple genetic resources, we surveyed 177 pineapple farmers in southern benin. we assessed farmers' knowledg ...201424936393
viscosity differences between thickened beverages suitable for elderly patients with dysphagia.dysphagia has been associated with a high risk of undernutrition and aspiration pneumonia in the elderly. it is commonly managed by adding commercial thickening agents to thicken liquids. the rheological behavior of these thickeners in water is known but there is lack of information with regard to other liquids. the aim of this study was to determine the viscosity of 11 beverages after adding commercial thickeners and to compare their viscosity with that of thickened water and also with the refe ...201424842337
utilization of agricultural residues of pineapple peels and sugarcane bagasse as cost-saving raw materials in scenedesmus acutus for lipid accumulation and biodiesel production.the aim of this study is to optimize the lipid accumulation in microalgae by using two agricultural residues of pineapple peels and sugarcane bagasse as low-cost organic carbon sources. green microalgae scenedesmus acutus was isolated and selected for cultivation. effects of three initial sugar concentrations and the stage for adding sugar during cultivation on biomass and lipid production were investigated. the results clearly showed that two-stage cultivation is more suitable than one-stage. t ...201424817554
rediscovering medicinal plants' potential with omics: microsatellite survey in expressed sequence tags of eleven traditional plants with potent antidiabetic properties.herbal medicines and traditionally used medicinal plants present an untapped potential for novel molecular target discovery using systems science and omics biotechnology driven strategies. since up to 40% of the world's poor people have no access to government health services, traditional and folk medicines are often the only therapeutics available to them. in this vein, north east (ne) india is recognized for its rich bioresources. as part of the indo-burma hotspot, it is regarded as an epicent ...201424802971
the answer was not a pineapple. 201424794615
water-insoluble fiber-rich fraction from pineapple peel improves intestinal function in hamsters: evidence from cecal and fecal indicators.pineapple peel, a byproduct of agricultural processing, contains high levels of water-insoluble fiber-rich fraction (wiff) (~42%, wt/wt). our previous work has demonstrated that cellulose, hemicellulose (xylan and xyloglucan), and pectic substances are the major polysaccharides of pineapple-peel wiff. based on its chemical composition and unique characteristics, we hypothesized that daily consumption of wiff would improve intestinal function in hamsters. male golden syrian hamsters were fed a di ...201424774071
deciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping, rna-seq and eqtl analyses.understanding the basis for volatile organic compound (voc) biosynthesis and regulation is of great importance for the genetic improvement of fruit flavor. lactones constitute an essential group of fatty acid-derived vocs conferring peach-like aroma to a number of fruits including peach, plum, pineapple and strawberry. early studies on lactone biosynthesis suggest that several enzymatic pathways could be responsible for the diversity of lactones, but detailed information on them remained elusive ...201424742100
on the physiology of normal swallowing as revealed by magnetic resonance imaging in real time.the aim of this study was to assess the physiology of normal swallowing using recent advances in real-time magnetic resonance imaging (mri). therefore ten young healthy subjects underwent real-time mri and flexible endoscopic evaluations of swallowing (fees) with thickened pineapple juice as oral contrast bolus. mri movies were recorded in sagittal, coronal, and axial orientations during successive swallows at about 25 frames per second. intermeasurement variation was analyzed and comparisons be ...201424693283
genomic analyses of the cam plant pineapple.the innovation of crassulacean acid metabolism (cam) photosynthesis in arid and/or low co2 conditions is a remarkable case of adaptation in flowering plants. as the most important crop that utilizes cam photosynthesis, the genetic and genomic resources of pineapple have been developed over many years. genetic diversity studies using various types of dna markers led to the reclassification of the two genera ananas and pseudananas and nine species into one genus ananas and two species, a. comosus ...201424692645
tonoplast lipid composition and proton pump of pineapple fruit during low-temperature storage and blackheart development.vacuole represents a major storage organelle playing vital roles in ph homoeostasis and cellular detoxification. the chemical and functional properties of tonoplast in response to chilling temperature and their roles in chilling injury are largely unknown. in the current study, lipid composition of tonoplast and the activities of two vacuolar proton pumps, h?-atpase (v-atpase) and h?-pyrophosphatase (v-ppase), were investigated in accordance with the development of blackheart, a form of chilling ...201424658889
evaluation of anti-inflammatory activity of pseudananas macrodontes (morr.) harms (bromeliaceae) fruit extract in rats.several species of the family bromeliaceae are characterized by the production of proteases in unusual amounts, especially in fruits. bromelain, an extract rich in cysteine endopeptidases obtained from ananas comosus l., and a few other proteases have been used as anti-inflammatory agents for some years, but bromelain is still mainly being used as alternative and/or complementary therapy to the treatment with glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. in this study, the ...201424601082
effect of pesticides used in banana and pineapple plantations on aquatic ecosystems in costa rica.current knowledge on fate and effect of agricultural pesticides comes is mainly from temperate ecosystems. more studies are needed in tropical systems in order to assess contamination risks to nontarget endemic tropical species from the extensive use of pesticides e.g. in banana and pineapple plantations. in this study, acute laboratory toxicity tests with organophosphate pesticides ethoprophos and chlorpyrifos were conducted on two costa rican species, cladoceran daphnia ambigua and fish parach ...201424579523
use of cholinesterase activity as a biomarker of pesticide exposure used on costa rican banana plantations in the native tropical fish astyanax aeneus (günther, 1860).in costa rica, thousands of tones of agricultural pesticides have been used for decades and their use is continuously increasing due to intensive and expanding production of coffee, pineapple, rice, ornamental plants and bananas. the objective of this study was to evaluate whether choline esterase (che) activity could be used as a biomarker of exposure to pesticides in the costa rican native fish astyanax aeneus (characidae). three methods used in order to evaluate the che biomarker were as foll ...201424579519
the effective use of acai juice, blueberry juice and pineapple juice as negative contrast agents for magnetic resonance cholangiopancreatography in children.magnetic resonance cholangiopancreatography (mrcp) is commonly performed in the evaluation of known or suspected pancreaticobiliary disease in children. the administration of a negative oral contrast agent can improve the quality of the examination without significant additional cost. we describe our experience with certain brands of acai juice, blueberry juice and pineapple juice as negative oral contrast agents in children. we believe these fruit juices are safe, palatable and may improve mrcp ...201424573534
physical, mechanical and hydration kinetics of particleboards manufactured with woody biomass (cupressus lusitanica, gmelina arborea, tectona grandis), agricultural resources, and tetra pak packages.lignocellulosic wastes resulting from agricultural activities as well as tetra pak residues from urban centres can cause significant levels of pollution. a possible action to minimize this problem is to use them in the production of particleboards. the purpose of this study was to evaluate the physical, mechanical, and hydration properties of particleboards manufactured with the mixture of woody biomass (cupressus lusitanica, gmelina arborea, and tectona grandis) and either agricultural wastes [ ...201424519224
influence of weight and type of planting material on fruit quality and its heterogeneity in pineapple [ananas comosus (l.) merrill].cultural practices can affect the quality of pineapple fruits and its variation. the objectives of this study were to investigate (a) effects of weight class and type of planting material on fruit quality, heterogeneity in quality and proportion and yield of fruits meeting european export standards, and (b) the improvement in quality, proportion and yield of fruits meeting export standards when flowering was induced at optimum time. experiments were conducted in benin with cvs sugarloaf (a perol ...201425653659
heterogeneity in pineapple fruit quality results from plant heterogeneity at flower induction.heterogeneity in fruit quality constitutes a major constraint in agri-food chains. in this paper the sources of the heterogeneity in pineapple in the field were studied in four experiments in commercial pineapple fields. the aims were to determine (a) whether differences in pineapple fruit quality among individual fruits are associated with differences in vigor of the individual plants within the crop at the time of artificial flower induction; and (b) whether the side shoots produced by the pla ...201425538714
[transmembrane transport behavior of in vitro hepg2 cells of ananas and its effect on lipids and glucose distribution].pineapple (ananas comosus) leaves contain mainly phenolic components with antioxidant and hypolipidemic effects. one of the principle components is p-coumaric acid. in this study, the transport behavior of p-coumaric acid, was observed after the administration of pineapple leaf phenols in vitro. simultaneously, the effect of the phenols on glucose, total cholesterol and triglycerides transportation and metabolism in hepg2 cells was also observed. the results showed that the phenols had good tran ...201425509303
effects of canned pineapple consumption on nutritional status, immunomodulation, and physical health of selected school children.this randomized, controlled trial examined the effects of canned pineapple consumption on immunomodulation, nutritional status, and physical health of ninety-eight (98) school children with mean age of 8.44 ± 0.20. the study participants were divided into three groups: group a (33) includes subjects who were not given canned pineapple, group b (33) includes those who were given 140 g, and group c (32) includes those given 280 g of canned pineapple for nine weeks. each major group was further div ...201425505983
amino acid profile as a feasible tool for determination of the authenticity of fruit juices.fruit juice is a nutrient rich food product with a direct connection to public health. the purpose of this research was to determine the amino acid profile of juices and provide a quick and accurate indicator for determining their authenticity.201425436191
species diversity and activity of insectivorous bats in three habitats in la virgen de sarapiquí, costa rica.pineapple farms make up 45,000 ha of costa rican landscape and are the second most exported crop. this is economically beneficial for the costa ricans, but greatly affects the natural flora and fauna because it is such a low growing crop. this study examined the differences in insectivorous bat species diversity and activity in the habitat gradient between the forest in tirimbina biological reserve in la virgen de sarapiquí, heredia, costa rica and the nearby pineapple farm called finca corsican ...201425412526
effects of different agricultural systems on soil quality in northern limón province, costa rica.conversion of native rainforest ecosystems in limón province of costa rica to banana and pineapple monoculture has led to reductions in biodiversity and soil quality. agroforestry management of cacao (theobroma cacao) is an alternative system that may maintain the agricultural livelihood of the region while more closely mimicking native ecosystems. this study compared physical, biological and chemical soil quality indicators of a cacao plantation under organic agroforestry management with banana ...201425412521
successful treatment with pineapple juice of a gastric bezoar caused by mastic. 201425409047
effects of elevated temperature postharvest on color aspect, physiochemical characteristics, and aroma components of pineapple fruits.in this work, 2 separate experiments were performed to describe the influence of elevated temperature treatments postharvest on the color, physiochemical characteristics and aroma components of pineapple fruits during low-temperature seasons. the l* (lightness) values of the skin and pulp of pineapple fruits were decreased. the a* (greenness-redness) and b* (blueness-yellowness) values of the skin and pulp were all markedly increased. the elevated temperature significantly increased the contents ...201425367439
evaluation of date syrup as an oral negative contrast agent for mrcp.the purpose of this study was to compare the in vitro effects of date syrup with those of other contrast agents by qualitative and quantitative analysis and in vivo evaluation of the use of date syrup to improve the quality of mrcp images.201425341137
interactive neonatal gastrointestinal magnetic resonance imaging using fruit juice as an oral contrast media.the objective was to evaluate the use of fruit juice with an interactive inversion recovery (ir) mr pulse sequence to visualise the gastrointestinal tract.201425245815
partitioning carbon dioxide emission and assessing dissolved organic carbon leaching of a drained peatland cultivated with pineapple at saratok, malaysia.pineapples (ananas comosus (l.) merr.) cultivation on drained peats could affect the release of carbon dioxide (co2) into the atmosphere and also the leaching of dissolved organic carbon (doc). carbon dioxide emission needs to be partitioned before deciding on whether cultivated peat is net sink or net source of carbon. partitioning of co2 emission into root respiration, microbial respiration, and oxidative peat decomposition was achieved using a lysimeter experiment with three treatments: peat ...201425215335
ananas comosus l. leaf phenols and p-coumaric acid regulate liver fat metabolism by upregulating cpt-1 expression.in this study, we aimed to investigate the effect and action mechanisms of pineapple leaf phenols (plps) on liver fat metabolism in high-fat diet-fed mice. results show that plp significantly reduced abdominal fat and liver lipid accumulation in high-fat diet-fed mice. the effects of plp were comparable with those of fb. furthermore, at the protein level, plp upregulated the expression of carnitine palmitoyltransferase 1 (cpt-1), whereas fb had no effects on cpt-1 compared with the hfd controls. ...201425197313
determination of some heavy metal levels in soft drinks on the ghanaian market using atomic absorption spectrometry method.twenty-three soft drink samples (i.e., four pineapple-based fruit drinks, eight citrus-based fruit juices, one soya-based drink, three cola carbonated drinks, one apple-based fruit drink, and six cocktail fruit drinks) were randomly purchased from retail outlets in an urban market in accra and analyzed for the concentrations of iron, cobalt, cadmium, zinc, lead, and copper using flame atomic absorption spectrometry. the mean concentration of iron and cadmium were 0.723 ± 0.448 mg/l and 0.032 ± 0 ...201425179943
the pineapple acmads1 promoter confers high level expression in tomato and arabidopsis flowering and fruiting tissues, but acmads1 does not complement the tomato lemads-rin (rin) mutant.a previous est study identified a mads box transcription factor coding sequence, acmads1, that is strongly induced during non-climacteric pineapple fruit ripening. phylogenetic analyses place the acmads1 protein in the same superclade as lemads-rin, a master regulator of fruit ripening upstream of ethylene in climacteric tomato. lemads-rin has been proposed to be a global ripening regulator shared among climacteric and non-climacteric species, although few functional homologs of lemads-rin have ...201425139231
bromelain surface modification increases the diffusion of silica nanoparticles in the tumor extracellular matrix.tumor extracellular matrix (ecm) represents a major obstacle to the diffusion of therapeutics and drug delivery systems in cancer parenchyma. this biological barrier limits the efficacy of promising therapeutic approaches including the delivery of sirna or agents intended for thermoablation. after extravasation due to the enhanced penetration and retention effect of tumor vasculature, typical nanotherapeutics are unable to reach the nonvascularized and anoxic regions deep within cancer parenchym ...201425119793
quality of osmotically pre-treated and vacuum dried pineapple cubes on storage as influenced by type of solutes and packaging materials.the quality and stability of osmotically pre-treated and subsequently vacuum dried pineapple cubes using three different solutes and packed in three different types of packaging materials on storage was evaluated. the experiment was laid out in completely randomized block design with two factors and three replications for each treatment. treatment combinations were considered as one factor and storage interval as another factor. pineapple cubes stored in glass bottle showed very little percentag ...201425114348
efficient determination of protocatechuic acid in fruit juices by selective and rapid magnetic molecular imprinted solid phase extraction coupled with hplc.magnetic molecular imprinted polymers (mmips) have been prepared as solid phase material to selectively extract protocatechuic acid (pca) from fruit juices with high capacity and fast binding kinetics. the resulting mmips were characterized by tem, ft-ir, tga, and vsm. the adsorption process between pca and mmips followed langumuir adsorption isotherm with maximum adsorption capacity at 7.5 mg/g and pseudo-second-order reaction kinetics with fast binding kinetics (equilibrium time at 40 min). in ...201425075753
the role of fragmentation and landscape changes in the ecological release of common nest predators in the neotropics.loss of large mammalian carnivores may allow smaller mesopredators to become abundant and threaten other community members. there is considerable debate about mesopredator release and the role that other potential factors such as landscape variables and human alterations to land cover lead to increased mesopredator abundance. we used camera traps to detect four mesopredators (tayra, eira barbara; white-nosed coati, nasua narica; northern raccoon, procyon lotor; and common opossum, didelphis opos ...201425071989
decolorization of palm oil mill effluent using growing cultures of curvularia clavata.agricultural wastewater that produces color are of environmental and health concern as colored effluent can produce toxic and carcinogenic by-products. from this study, batch culture optimization using response surface methods indicated that the fungus isolated from the pineapple solid waste, curvularia clavata was able to decolorize sterile palm oil mill effluent (pome) which is mainly associated with polyphenol and lignin. results showed successful decolorization of pome up to 80 % (initial ad ...201424327114
functional properties of a cysteine proteinase from pineapple fruit with improved resistance to fungal pathogens in arabidopsis thaliana.in plant cells, many cysteine proteinases (cps) are synthesized as precursors in the endoplasmic reticulum, and then are subject to post-translational modifications to form the active mature proteinases. they participate in various cellular and physiological functions. here, accp2, a cp from pineapple fruit (ananas comosus l.) belonging to the c1a subfamily is analyzed based on the molecular modeling and homology alignment. transcripts of accp2 can be detected in the different parts of fruits (p ...201424566309
a standardised challenge model with an enterotoxigenic f4+ escherichia coli strain in piglets assessing clinical traits and faecal shedding of fae and est-ii toxin genes.this study evaluated the effect of five feed additives on post weaning diarrhoea (pwd) in piglets challenged 3 d after weaning with an enterotoxigenic escherichia coli strain (etec). in three experimental runs, a total of 84 piglets was weaned at 21 days of age and randomly assigned to seven treatments. as dietary treatment, piglets were fed a basal diet or diets with addition of bovine colostrum (0.2%), pineapple stem extract containing bromelain (0.2%), an autolysed yeast preparation (saccharo ...201425313936
characterization of the third serk gene in pineapple (ananas comosus) and analysis of its expression and autophosphorylation activity in vitro.two somatic embryogenesis receptor-like kinase genes (identified as acserk1 and acserk2) have previously been characterized from pineapple (ananas comosus). in this work, we describe the characterization of a third gene (acserk3) in this family. acserk3 had all the characteristic domains and shared extensive sequence homology with other plant serks. acserk3 expression was studied by in situ hybridization and quantitative real-time pcr to analyze its function. intense in situ hybridization signal ...201425249776
evaluation of antibacterial activities of silver nanoparticles green-synthesized using pineapple leaf (ananas comosus).pineapple leaf was used in this study for the synthesis of silver nanoparticles based on the search for sustainable synthetic means. indeed, this offered an economical and sustainable synthetic route relative to expensive and toxic chemical methods. the leaf extract was used and the corresponding nanoparticles obtained were subjected to uv-vis analysis at different times. the uv-vis was used to monitor the silver nanoparticle formation through sampling at time intervals. the formation of silver ...201424268599
functional characterization of recombinant bromelain of ananas comosus expressed in a prokaryotic system.bromelain (brm) is a defense protein present in the fruit and stem of pineapple (ananas comosus) and it is grouped as a cysteine protease enzyme with diversified medicinal uses. based on its therapeutic applications, bromelain has got sufficient attention in pharmaceutical industries. in the present study, the full coding gene of bromelain in pineapple stem (1,093 bp) was amplified by rt-pcr. the pcr product was cloned, sequenced, and characterized. the sequence analysis of the gene revealed the ...201423921698
fresh-cut pineapple as a new carrier of probiotic lactic acid bacteria.due to the increasing interest for healthy foods, the feasibility of using fresh-cut fruits to vehicle probiotic microorganisms is arising scientific interest. with this aim, the survival of probiotic lactic acid bacteria, belonging to lactobacillus plantarum and lactobacillus fermentum species, was monitored on artificially inoculated pineapple pieces throughout storage. the main nutritional, physicochemical, and sensorial parameters of minimally processed pineapples were monitored. finally, pr ...201425093163
benefit of supplements in functional dyspepsia after treatment of helicobacter pylori.functional dyspepsia, though benign, leads to deterioration of the quality of life and high costs for healthcare systems. the optimal therapy for functional dyspepsia is still to be defined because of its multifactorial pathogenesis. in an open multicentric study of patients with functional dyspepsia, we prospectively evaluated the benefit of treatment with a food supplement composed of sodium alginate, carbonate calcium, pineapple, papaya, ginger, α-galactosidase and fennel (perdiges, bioten sn ...201425384804
fluctuation of diptera larvae in phytotelmata and relation with climate variation in west sumatra indonesia.research of fluctuations in diptera's larvae in phytotelmata had been conducted at three locations in west sumatra, indonesia; padang, bukittinggi and payakumbuh; which aimed to determine the number and fluctuations diptera larvae in phytotelmata. the results obtained; the highest number of individual larvae diptera in phytotelmata was 7109 aedes albopictus larvae (49.56%), followed by larvae of culex tritaeniorhynchus with 2409 individuals (16.80%). larvae fluctuated every month and tent to inc ...201426035947
survey of quality indicators in commercial dehydrated fruits.physical and chemical quality parameters (dry matter, aw, protein, carbohydrates, vitamin c, 2-furoylmethyl amino acids, rehydration ratio and leaching loss) have been determined in 30 commercial dehydrated fruits (strawberry, blueberry, raspberry, cranberry, cherry, apple, grapefruit, mango, kiwifruit, pineapple, melon, coconut, banana and papaya). for comparison purposes, strawberry samples processed in the laboratory by freeze-drying and by convective drying were used as control samples. over ...201424360417
characterization of mexican coriander (eryngium foetidum) essential oil and its inactivation of listeria monocytogenes in vitro and during mild thermal pasteurization of pineapple juice.the aim of this work was to characterize the essential oil (eo) of eryngium foetidum (efeo) and assess its activity toward listeria monocytogenes in broth and during thermal inactivation of the pathogen in pineapple juice. in this respect, efeo was chemically characterized, and its antilisteria potential in broth as a function of ph, cell load, and efeo concentration was assessed through a central composite design. furthermore, the inactivation kinetics of l. monocytogenes in the juice were asse ...201424674435
metabolic responses of lactobacillus plantarum strains during fermentation and storage of vegetable and fruit juices.strains of lactobacillus plantarum were grown and stored in cherry (chj), pineapple (pj), carrot (cj), and tomato (tj) juices to mimic the chemical composition of the respective matrices. wheat flour hydrolysate (wfh), whey milk (w), and mrs broth were also used as representatives of other ecosystems. the growth rates and cell densities of l. plantarum strains during fermentation (24 h at 30°c) and storage (21 days at 4°c) differed only in part, being mainly influenced by the matrix. chj and pj ...201424487533
a novel low-temperature-active pectin methylesterase from penicillium chrysogenum f46 with high efficiency in fruit firming.a pectin methylesterase gene (pe8f46) was cloned from penicillium chrysogenum f46 and successfully expressed in pichia pastoris. the full-length cdna consists of 969 bp and encodes a 322-residue polypeptide with the calculated molecular weight of 34.1 kda. deduced pe8f46 belongs to family 8 of carbohydrate esterases and shares 54% identity with a functionally characterised counterpart from myceliophthora thermophile. purified recombinant pe8f46 showed the optimal activity at ph 5.0 and 40°c, and ...201424874380
in vivo effect of selected medicinal plants against gastrointestinal nematodes of sheep.nematode resistance to anthelmintic drugs affects small ruminant production globally. this study evaluated in vivo effects of five plant extracts as alternative nematode treatments. animals were divided into six groups. each group was randomly assigned a treatment: abamectin and praziquantel (cap) (positive control), ethanol extracts of ananas comosus (ac), aloe ferox (af), allium sativum (as), lespedeza cuneata (lc) and warburgia salutaris (ws). these were applied as an oral dose (100 mg kg(-1) ...201424293151
the association between chromaticity, phenolics, carotenoids, and in vitro antioxidant activity of frozen fruit pulp in brazil: an application of chemometrics.a total of 19 brazilian frozen pulps from the following fruits: açai (euterpe oleracea), blackberry (rubus sp.), cajá (spondias mombin), cashew (anacardium occidentale), cocoa (theobroma cacao), coconut (cocos nucifera), grape (vitis sp.), graviola (annona muricata), guava (psidium guajava), papaya (carica papaya), peach (prunus persica), pineapple (ananas comosus), pineapple and mint (a. comosus and mentha spicata), red fruits (rubus sp. and fragaria sp.), seriguela (spondias purpurea), strawbe ...201424547813
highly thermostable and ph-stable cellulases from aspergillus niger ns-2: properties and application for cellulose hydrolysis.optimization of cultural conditions for enhanced cellulase production by aspergillus niger ns-2 were studied under solid-state fermentation. significant increase in yields (cmcase 463.9 ± 20.1 u/g, fpase 101.1 ± 3.5 u/g and β-glucosidase 99 ± 4.0 u/g) were obtained under optimized conditions. effect of different nutritional parameters was studied to induce the maximum production of cellulase complex. scale-up studies for enzyme production process were carried out. characterization studies showed ...201424052336
in vitro evaluation of antibacterial efficacy of pineapple extract (bromelain) on periodontal pathogens.periodontitis is an inflammatory disease resulting in the destruction of periodontal tissues. various treatment modalities have been tried in the form of mechanical therapy and surgical therapy. antimicrobial agents have been used as a monotherapy and as an adjunct with mechanical debridement. various plant extracts have been used as antibacterial agents. pineapple extract (bromelian) is one such agent. hence this study was conducted to assess the antibacterial efficacy of bromelain on both aero ...201425395802
the sugarcane defense protein sugarwin2 causes cell death in colletotrichum falcatum but not in non-pathogenic fungi.plants respond to pathogens and insect attacks by inducing and accumulating a large set of defense-related proteins. two homologues of a barley wound-inducible protein (barwin) have been characterized in sugarcane, sugarwin1 and sugarwin2 (sugarcane wound-inducible proteins). induction of sugarwins occurs in response to diatraea saccharalis damage but not to pathogen infection. in addition, the protein itself does not show any effect on insect development; instead, it has antimicrobial activitie ...201424608349
draft genome sequence of pantoea ananatis strain lmg 2665t, a bacterial pathogen of pineapple fruitlets.we report the draft genome sequence of pantoea ananatis lmg 2665(t), the bacterial causal agent of pineapple fruitlet rot.201424855311
fungal presence in selected tree nuts and dried fruits.sixty-four tree nut samples (almonds, pecans, pine nuts, and walnuts) and 50 dried fruit samples (apricots, cranberries, papaya, pineapple, and raisins) were purchased from local supermarkets and analyzed for fungal contamination using conventional culture as well as molecular methods. the results of our study showed that the highest yeast and mold (ym) counts (5.34 log10 cfu g(-1)) were found in walnuts and the lowest in pecans. the most common mold in nuts was aspergillus niger, relatively low ...201526056470
occurrence of cellulose-producing gluconacetobacter spp. in fruit samples and kombucha tea, and production of the biopolymer.cellulose producing bacteria were isolated from fruit samples and kombucha tea (a fermented beverage) using cuso4 solution in modified watanabe and yamanaka medium to inhibit yeasts and molds. six bacterial strains showing cellulose production were isolated and identified by 16s rrna gene sequencing as gluconacetobacter xylinus strain dfbt, ga. xylinus strain dfr-1, gluconobacter oxydans strain dfr-2, g. oxydans strain dfr-3, acetobacter orientalis strain dfr-4, and gluconacetobacter intermedius ...201525926011
applications of microencapsulated bifidobacterium longum with eleutherine americana in fresh milk tofu and pineapple juice.bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. microencapsulation of b. longum with eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. microencapsulated b. longum with the extract and oligosaccharides extract in the food products showed better sur ...201525854832
isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico.mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ...201526405529
analysis of pineapple mealybug wilt associated virus -1 and -2 for potential rna silencing suppressors and pathogenicity factors.higher plants use rna silencing to defend against viral infections. as a counter defense, plant viruses have evolved proteins that suppress rna silencing. mealybug wilt of pineapple (mwp), an important disease of pineapple, has been associated with at least three distinct viruses, pineapple mealybug wilt associated virus -1, -2, and -3 (pmwav-1, -2, and -3). selected open reading frames (orfs) of pmwav-1 and pmwav-2 were screened for their local and systemic suppressor activities in agrobacteriu ...201525751306
growth potential of listeria monocytogenes and staphylococcus aureus on fresh-cut tropical fruits.the objective of this study was to evaluate the fate of staphylococcus aureus, listeria monocytogenes, and natural microbiota on fresh-cut tropical fruits (pitaya, mango, papaya and pineapple) with commercial pvc film at different storage temperature (5, 13, and 25 °c). the results showed that s. aureus, l. monocytogenes, and natural microbiota increased significantly on fresh-cut tropical fruits at 25 °c. both pathogen and natural microbiota were able to grow on fresh-cut tropical fruits at 13 ...201526862605
phenotypic and molecular diversity of meyerozyma guilliermondii strains isolated from food and other environmental niches, hints for an incipient speciation.meyerozyma guilliermondii is a yeast species widely isolated from several natural environments and from fruit; in medical microbiology it is known as the teleomorph of the opportunistic pathogen candida guilliermondii, which causes about 2% of the human blood infections. this yeast is also promising in a variety of biotechnological applications as vitamins production and post-harvest control. the question if isolates from different sources are physiologically and genetically similar, or if the v ...201525791010
pantoea ananatis utilizes a type vi secretion system for pathogenesis and bacterial competition.type vi secretion systems (t6sss) are a class of macromolecular machines that are recognized as an important virulence mechanism in several gram-negative bacteria. the genome of pantoea ananatis lmg 2665(t), a pathogen of pineapple fruit and onion plants, carries two gene clusters whose predicted products have homology with t6ss-associated gene products from other bacteria. nothing is known regarding the role of these t6ss-1 and t6ss-3 gene clusters in the biology of p. ananatis. here, we presen ...201525411959
the relative anthelmintic efficacy of plant-derived cysteine proteinases on intestinal nematodes.we examined the in vitro and in vivo efficacy of plant cysteine proteinases (cps) derived from pineapple (ananas comosus) and kiwi fruit (actinidia deliciosa), and compared their efficacy as anthelmintics to the known effects of cps from the latex of papaya (carica papaya) against the rodent intestinal nematode, heligmosomoides bakeri. both fruit bromelain and stem bromelain had significant in vitro detrimental effects on h. bakeri but in comparison, actinidain from kiwi fruit had very little ef ...201524176056
application of membrane separation in fruit and vegetable juice processing: a review.fruit and vegetable juices are used due to convenience. the juices are rich in various minerals, vitamins, and other nutrients. to process the juices and their clarification and/or concentration is required. the membranes are being used for these purposes. these processes are preferred over others because of high efficiency and low temperature. membranes and their characteristics have been discussed in brief for knowing suitability of membranes for fruit and vegetable juices. membrane separation ...201524915352
dengue viruses in aedes albopictus skuse from a pineapple plantation in costa rica. 201526047200
agave as a model cam crop system for a warming and drying world.as climate change leads to drier and warmer conditions in semi-arid regions, growing resource-intensive c3 and c4 crops will become more challenging. such crops will be subjected to increased frequency and intensity of drought and heat stress. however, agaves, even more than pineapple (ananas comosus) and prickly pear (opuntia ficus-indica and related species), typify highly productive plants that will respond favorably to global warming, both in natural and cultivated settings. with nearly 200 ...201526442005
effect of refrigerated storage on probiotic viability and the production and stability of antimutagenic and antioxidant peptides in yogurt supplemented with pineapple peel.fruit by-products are good resources of carbohydrates, proteins, vitamins, and minerals, which may function as growth nutrients for probiotic bacteria. this research aimed at evaluating effects of pineapple peel powder addition on the viability and activity of lactobacillus acidophilus (atcc 4356), lactobacillus casei (atcc393), and lactobacillus paracasei ssp. paracasei (atcc baa52) in yogurts throughout storage at 4°c for 28d. plain and probiotic yogurts supplemented with or without pineapple ...201526142843
molecular cloning and characterization of udp-glucose: furaneol glucosyltransferase gene from grapevine cultivar muscat bailey a (vitis labrusca × v. vinifera).2,5-dimethyl-4-hydroxy-3(2h)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from american grape (vitis labrusca) and its hybrid grape. furaneol glucoside was recently isolated as an important furaneol derivative from the hybrid grapevine cultivar, muscat b ...201526160581
dai nippon printing co., ltd medi·ca cc for enumeration of coliform bacteria.a ready-made dry medium method for coliform count, the medi·ca cc method, was compared to the violet red bile agar method (bacteriological analytical manual, chapter 4, enumeration of escherichia coli and the coliform bacteria, section g) for nine raw foods from four food categories: raw ground pork, raw lamb, raw ground chicken, raw tuna fillet, raw salmon fillet, raw shrimp, fresh peeled banana, fresh cut pineapple, and fresh cut apple. the 95% confidence interval for the mean difference betwe ...201525857880
host extract modulates metabolism and fumonisin biosynthesis by the plant-pathogenic fungus fusarium proliferatum.fusarium proliferatum is a common pathogen able to infect a broad range of agriculturally important crops. recently, some evidence for genetic variance among the species genotypes in relation to their plant origin has been reported. mycotoxin contamination of plant tissues is the most important threat caused by f. proliferatum and fumonisins b (fbs) are the principal mycotoxins synthesized. the toxigenic potential of the pathogen genotypes is variable and also the reaction of different host plan ...201525462926
phenylpropanoid glycosides from the leaves of ananas comosus.two new phenylpropanoid glycosides, named β-d-(1-o-acetyl-3,6-o-diferuloyl) fructofuranosyl β-d-6'-o-acetylglucopyranoside (1) and β-d-(1-o-acetyl-3,6-o-diferuloyl) fructofuranosyl α-d-glucopyranoside (2), along with two known analogues (3-4) and four glycerides (5-8), were isolated from the etoac extract of the leaves of ananas comosus. their structures were elucidated on the basis of 1d- and 2d-nmr analyses, as well as hr-esi-ms experiments. compounds 1-4 showed significant antibacterial activ ...201526882684
authentication of pineapple (ananas comosus [l.] merr.) fruit maturity stages by quantitative analysis of γ- and δ-lactones using headspace solid-phase microextraction and chirospecific gas chromatography-selected ion monitoring mass spectrometry (hs-spme-gc-sim-ms).headspace solid phase microextraction and chirospecific gas chromatography-mass spectrometry in selected ion monitoring mode (hs-spme-gc-sim-ms) allowed quantitative determination of δ-lactones (δ-c8, δ-c10) and γ-lactones (γ-c6, γ-c8, γ-c10). a stable isotope dilution assay (sida) with d7-γ-decalactone as internal standard was used for quantitative analysis of pineapple lactones that was performed at three progressing post-harvest stages of fully ripe air-freighted and green-ripe sea-freighted ...201525172740
application of diffusion ordered-1h-nuclear magnetic resonance spectroscopy to quantify sucrose in beverages.this work focuses on a quantitative analysis of sucrose using diffusion ordered-quantitative (1)h-nuclear magnetic resonance spectroscopy (dosy-qnmr), where an analyte can be isolated from interference based on its characteristic diffusion coefficient (d) in gradient magnetic fields. the d value of sucrose in deuterium oxide at 30°c was 4.9 × 10(-10)m(2)/s at field gradient pulse from 5.0 × 10(-2) to 3.0 × 10(-1)t/m, separated from other carbohydrates (glucose and fructose). good linearity (r(2) ...201525308635
transient elevation of serum 5-hiaa by dietary serotonin and distribution of 5-hiaa in serum protein fractions.dietary serotonin increases urinary secretion of 5-hiaa. a falsely elevated 5-hiaa may lead to incorrect suspicion of a neuroendocrine tumour. therefore, we determined the effect and duration of dietary serotonin on serum 5-hiaa concentration. we also studied the distribution of 5-hiaa in serum fractions.201525249663
swarm motility of salmonella enterica serovar typhimurium is inhibited by compounds from fruit peel extracts.controlling spread of human pathogens on fresh produce is a top priority for public health reasons. isolation of compounds from agricultural waste that would control spread of human pathogens was explored using salmonella enterica serovar typhimurium as a model organism. in the environment, micro-organisms migrate as a 'community' especially when they move on moist surfaces. this type of motility is characterized as swarming motility. we examined extracts from agricultural waste such as soya bea ...201525422036
pineapple [ananas comosus (l.) merr].the efficacy of agrobacterium-mediated pineapple transformation technique has been improved (mean percentage of transgenic micro-shoots regenerated from initial callus explants up to 20.6%) using a novel encapsulation-based, antibiotic selection procedure. the detailed protocol using a standard plant transformation vector (pcambia1304) as reported in an 'elite' indian variety (queen) of pineapple [ananas comosus (l.) merr] can be applied to other varieties of pineapple for introgression of targe ...201525416266
phylogeography of pink pineapple mealybugs, dysmicoccus brevipes (cockerell) reveals the history of pineapple introduction and cultivation in china.the pink pineapple mealybug (ppm), dysmicoccus brevipes (cockerell) (hemiptera: pseudococcidae), is a widespread plant-sucking insect of considerable concern because it transmits the pineapple mealybug wilt-associated virus. its distribution is closely linked with its host, the pineapple [ananas comosus (l.) merrill] because of its wingless and parthenogenetic characteristics. to investigate the history of d. brevipes introduction and the cultivation of pineapple in china, samples of d. brevipes ...201526345923
kinetics studies with fruit bromelain (ananas comosus) in the presence of cysteine and divalent ions.the kinetics of cysteine and divalent ion modulation viz. ca(2+), cu(2+), hg(2+) of fruit bromelain (ec 3.4.22.33) have been investigated in the present study. kinetic studies revealed that at ph 4.5, cysteine induced v-type activation of bromelain catalyzed gelatin hydrolysis. at ph 3.5, ca(2+) inhibited the enzyme noncompetitively, whereas, both k-and v-type activations of bromelain were observed in the presence of 0.5 mm ca(2+) at ph 4.5 and 7.5. bromelain was inhibited competitively at 0.6 m ...201526345013
patulin and its dietary intake by fruit juice consumption in iran.a survey was conducted to determine levels and dietary intake of patulin (pat) from fruit juices consumed in iran. pat content was determined using high performance liquid chromatography-diode array detection in 161 samples of fruit juices including apple, pineapple, pear, peach, pomegranate and white and red grape juices. results revealed the presence of pat in 16.1% of the samples examined, ranging from 5 to 190.7 µg/kg. average and median pat content in positive samples was 34.5 and 18.5 µg/k ...201525494337
ripening-dependent metabolic changes in the volatiles of pineapple (ananas comosus (l.) merr.) fruit: ii. multivariate statistical profiling of pineapple aroma compounds based on comprehensive two-dimensional gas chromatography-mass spectrometry.ripening-dependent changes of pineapple volatiles were studied in a nontargeted profiling analysis. volatiles were isolated via headspace solid phase microextraction and analyzed by comprehensive 2d gas chromatography and mass spectrometry (hs-spme-gc×gc-qms). profile patterns presented in the contour plots were evaluated applying image processing techniques and subsequent multivariate statistical data analysis. statistical methods comprised unsupervised hierarchical cluster analysis (hca) and p ...201525651901
study on evaluation of silage from pineapple (ananas comosus) fruit residue as livestock feed.pineapple is a commercially important fruit crop grown in asian and african countries. pineapple fruit residue (pfr) accounts for more than 65% of the processed fruits, and its disposal is a major problem due to its high moisture and sugar content predisposing it to fungal growth and spoilage. silage technique was adopted to address this problem, and the pfr silage was evaluated for its feeding value. it was observed that on 15th day, the ph of pfr silage was 4.2-4.3 and lactic acid content was ...201525633915
a new lavandulol-related monoterpene in the sex pheromone of the grey pineapple mealybug, dysmicoccus neobrevipes.the grey pineapple mealybug, dysmicoccus neobrevipes, is a serious pest that attacks a variety of crops in tropical regions. recently, it was recorded on an island in southwestern japan, suggesting that its distribution is expanding. as a measure against this expansion, a monitoring tool is urgently needed. in this study we determined the structure of the sex pheromone of d. neobrevipes in order to develop an efficient lure for monitoring traps. volatiles collected from virgin adult females were ...201525618324
immune reactivities against gums.different kinds of gums from various sources enjoy an extremely broad range of commercial and industrial use, from food and pharmaceuticals to printing and adhesives. although generally recognized as safe by the us food and drug administration (fda), gums have a history of association with sensitive or allergic reactions. in addition, studies have shown that gums have a structural, molecular similarity to a number of common foods. a possibility exists for cross-reactivity.201525599187
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