Publications

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effects of a killed-cover crop mulching system on sweetpotato production, soil pests, and insect predators in south carolina.sweetpotatoes, ipomoea batatas (l.) lam. (convolvulaceae), are typically grown on bare soil where weeds and erosion can be serious problems. conservation tillage systems using cover crop residues as mulch can help reduce these problems, but little is known about how conservation tillage affects yield and quality of sweetpotato or how these systems impact populations of beneficial and pest insects. therefore, field experiments were conducted at the u.s. vegetable laboratory, charleston, sc, in 20 ...200819133469
a randomized controlled trial of homeopathic treatment of weaned piglets in a commercial swine herd.to evaluate the zootechnic performance and occurrence of diarrhea in piglets in the week post-weaning comparing supplementation with sucrose saline which contained or did not contain added homeopathic medicine.200819371569
selecting and evaluating native plants for region-specific phytotoxicity testing.in this study, we evaluated methodology to determine risks to terrestrial native plant species from potential herbecide drift, focusing on 1) selection of native species for testing, 2) growth of these species, and 3) variability in herbicide response among native species and compared with crop plants. native plant species were selected for initial testing on the basis of spatial analysis, which indicated that species from illinois, usa, were at potential risk for off-target effects of herbicide ...200817973566
perturbation of the ground-state electronic structure of fmn by the conserved cysteine in phototropin lov2 domains.in lov2, the blue-light sensitive domain of phototropin, the primary photophysical event involves intersystem crossing (isc) from the singlet-excited state to the triplet state. the isc rate is enhanced in lov2 as compared to flavin mononucleotide (fmn) in solution, which likely results from a heavy-atom effect of a nearby conserved cysteine, c450. here, we applied fluorescence line narrowing (fln), resonance raman (rr) and fourier-transform infrared (ftir) spectroscopy to investigate the electr ...200818989482
effects of vegetable oil residue after soil extraction on physical-chemical properties of sandy soil and plant growth.vegetable oil has the ability to extract polycyclic aromatic hydrocarbons (pahs) from contaminated sandy soil for a remediation purpose, with some of the oil remaining in the soil. although most of the pahs were removed, the risk of residue oil in the soil was not known. the objective of this study was to evaluate the effects of the vegetable oil residue on higher plant growth and sandy soil properties after soil extraction for a better understanding of the soil remediation. addition of sunflowe ...200819209632
a conserved glutamine plays a central role in lov domain signal transmission and its duration.light is a key stimulus for plant biological functions, several of which are controlled by light-activated kinases known as phototropins, a group of kinases that contain two light-sensing domains (lov, light-oxygen-voltage domains) and a c-terminal serine/threonine kinase domain. the second sensory domain, lov2, plays a key role in regulating kinase enzymatic activity via the photochemical formation of a covalent adduct between a lov2 cysteine residue and an internally bound flavin mononucleotid ...200819063612
carbon partitioning between oil and carbohydrates in developing oat (avena sativa l.) seeds.cereals accumulate starch in the endosperm as their major energy reserve in the grain. in most cereals the embryo, scutellum, and aleurone layer are high in oil, but these tissues constitute a very small part of the total seed weight. however, in oat (avena sativa l.) most of the oil in kernels is deposited in the same endosperm cells that accumulate starch. thus oat endosperm is a desirable model system to study the metabolic switches responsible for carbon partitioning between oil and starch s ...200819036843
reproduction and feeding behavior of myzus persicae on four cereals.green peach aphid, myzus persicae (sulzer), does not overwinter outdoors in minnesota; it arrives each spring on low-level jet streams from the south. after arrival, anholocylic reproduction occurs on numerous herbaceous species, including many common weeds, before movement to potato, solanum tuberosum l. in investigating aphid feeding behavior on barrier crops, we observed winter wheat, triticum aestivum l., colonized by green peach aphid. the northern great plains grows 94,000 ha of potatoes a ...200818330110
evaluating wastewater-induced plant genotoxicity using randomly amplified polymorphic dna.wastewater often contains genotoxic substances that can resist different stages of the treatment process. in the present study, randomly amplified polymorphic dna technology was applied to evaluate the genotoxic effects of wastewater (treated and raw) irrigation on oat plants (avena sativa). rapd profiles obtained showed that both treated and raw wastewater (rww) were having genotoxic effects on oat plants. this was apparent by the appearance/disappearance of bands in the treatments compared wit ...200818214923
discrete developmental roles for temperate cereal grass vernalization1/fruitfull-like genes in flowering competency and the transition to flowering.members of the grass subfamily pooideae are characterized by their adaptation to cool temperate climates. vernalization is the process whereby flowering is accelerated in response to a prolonged period of cold. winter cereals are tolerant of low temperatures and flower earlier with vernalization, whereas spring cultivars are intolerant of low temperatures and flower later with vernalization. in the pooid grasses wheat (triticum monococcum, triticum aestivum) and barley (hordeum vulgare), vernali ...200818024551
analysis of oil composition in cultivars and wild species of oat (avena sp.).oil quality and content were analyzed in 33 accessions from 13 wild species and 10 accessions of cultivated oat. wild oat species tended to have higher oil and 18:1 fatty acid (fa) contents and lower amounts of 18:2 and 18:3 fas as compared to cultivated oats. in addition to common fas, minor amounts of several hydroxy and epoxy fas were also present in the oat oil and mainly confined to specific lipid classes. these unusual fas included the previously reported 15-hydroxy 18:2 (delta9,12) (aveno ...200818707115
the first doubled haploid linkage map for cultivated oat.to date, all linkage maps of hexaploid oat (avena sativa l.) have been constructed using recombinant inbred lines (rils). doubled haploids (dhs), however, have the advantage over rils of their comprehensive homozygosity. dhs have been used for mapping in several cereal species, but in oats the production of large dh populations has only recently become an option. a linkage map of hexaploid oat was constructed using an anther culture-derived dh population (137 individuals) from the f1 individuals ...200818650946
genetic diversity among oat varieties of worldwide origin and associations of aflp markers with quantitative traits.one hundred and fourteen oat (avena sativa l.) varieties of worldwide origin were evaluated for genetic diversity based on 77 molecular polymorphisms produced by eight selective aflp primer combinations. genetic similarity, calculated using the dice coefficient, was used for cluster analysis and principal component analysis was applied. in addition population structure was explored to identify discrete subpopulations based on allele frequency. although clustering and population structure showed ...200818633590
yield of four agaricus bisporus strains in three compost formulations and chemical composition analyses of the mushrooms.three compost formulations, consisting of two varieties of cynodom dactylon (l.) pers. (coast-cross and tyfton) and oat (avena sativa) straw were tested for the cultivation of a. bisporus strains abi-01/01, abi-04/02, abi-05/03, and abi-06/04. a completely randomized experimental design in a factorial scheme was adopted, with 12 treatments (4 a. bisporus strains × 3 types of compost) and 8 replicates. each experimental unit corresponded to one box containing 12 - 12.5 kg fresh wet compost. the d ...200824031271
can artificial soil be used in the vegetative vigor test for u.s. pesticide registration?current testing guidelines for pesticide registration for the protection of nontarget plants calls for the use of sterilized, standardized soil consisting of primarily sandy loam, loamy sand, loamy clay, or clay loam that contains up to 3% organic matter. low organic matter soils can be difficult to manage in a greenhouse setting because when soils dry, they contract, causing impeded water infiltration, or when overwatered, poor drainage increases the chances of anaerobic conditions. the purpose ...200818563958
fusion of oil bodies in endosperm of oat grains.few microscopical studies have been made on lipid storage in oat grains, with variable results as to the extent of lipid accumulation in the starchy endosperm. grains of medium- and high-lipid oat (avena sativa l.) were studied at two developmental stages and at maturity, by light microscopy using different staining methods, and by scanning and transmission electron microscopy. discrete oil bodies occurred in the aleurone layer, scutellum and embryo. in contrast, oil bodies in the starchy endosp ...200818563438
bioavailability of chemical pollutants in contaminated soils and pitfalls of chemical analyses in hazard assessment.decision-making for remediation of industrial wastelands are still based on the concentrations of pollutants of concern measured in soils. in this work, two soils polluted by polycyclic aromatic hydrocarbons (pahs) and metals were investigated for their toxicity on earthworms (eisenia fetida), collembolae (folsomia candida), and higher plants (brassica chinensis, lactucca sativa and avena sativa) in order to study the relationships between chemical contamination and biological effects. although ...200818561306
development of pcr-based scar and caps markers linked to beta-glucan and protein content qtl regions in oat.a key breeding objective in oat (avena sativa l.) is cultivars with high and low beta-glucan content. in a targeted strategy to develop pcr-based markers linked to published beta-glucan content quantitative trait loci (qtls) regions, 15 random amplified polymorphic dna (rapd) fragments were cloned and their sequences used to design sequence-characterized amplified region (scar) and cleaved amplified polymorphic sequence (caps) primers. the 13 derived scar markers and 2 derived caps markers were ...200818521120
biological testing of a digested sewage sludge and derived composts.aiming to evaluate a possible loss of soil habitat function after amendment with organic wastes, a digested sewage sludge and derived composts produced with green residues, where biologically tested in the laboratory using soil animals (eisenia andrei and folsomia candida) and plants (brassica rapa and avena sativa). each waste was tested mimicking a field application of 6ton/ha or 12ton/ha. avoidance tests did not reveal any impact of sludge and composts to soil biota. germination and growth te ...200818413285
line-scanning microscopy for time-gated and spectrally resolved fluorescence imaging.laser-scanning fluorescence microscopy for efficient acquisition of time-gated and spectrally resolved fluorescence images was developed based on line illumination of the laser beam and detection of the fluorescence image through a slit. in this optical arrangement, the fluorescence image was obtained by scanning only one axis perpendicular to the excitation line, and the acquisition time was significantly reduced compared with conventional laser-scanning confocal microscopy. a multidimensional ...200819669492
[effect of strengthening solar ultraviolet b band irradiation on oat (avena sativa l. ) yield and its components in qing tibetan plateau].stratospheric ozone depletion occurs mainly over polar regions during the spring when the solar ultraviolet b-band (280-315 nm, uv-b) radiation is most intense in a year, but over the qing tibetan plateau region, the highest intensity is from june to september when the amount of uv-b radiation reaching the regions is more than that in the adjacent areas lying in the same latitude by 10%. from june to september is just the time of plant's germination, development, and reproduction in the alpine r ...200919839346
angiotensin-i converting enzyme inhibitory activity of hydrolysates from oat (avena sativa) proteins by in silico and in vitro analyses.the potential for producing antihypertensive peptides from oat proteins through enzymatic hydrolysis was assessed in silico and confirmed in vitro. thermolysin (ec 3.4.24.27) was predicted using biopep database as the enzyme that would theoretically produce the most angiotensin i converting enzyme (ace) inhibitory peptides from oat. experimental evidence confirmed that strong ace-inhibitory activity was produced under various hydrolysis conditions. hydrolysates produced under high enzyme-to-subs ...200919731915
addition of individual chromosomes of maize inbreds b73 and mo17 to oat cultivars starter and sun ii: maize chromosome retention, transmission, and plant phenotype.oat-maize addition (oma) lines with one, or occasionally more, chromosomes of maize (zea mays l., 2n = 2x = 20) added to an oat (avena sativa l., 2n = 6x = 42) genomic background can be produced via embryo rescue from sexual crosses of oat x maize. self-fertile disomic addition lines of different oat genotypes, mainly cultivar starter, as recipient for maize chromosomes 1, 2, 3, 4, 5, 6, 7, 9, and the short arm of 10 and a monosomic addition line for chromosome 8, have been reported previously i ...200919707741
nitrogen fertilizer effects on soil carbon balances in midwestern u.s. agricultural systems.a single ecosystem dominates the midwestern united states, occupying 26 million hectares in five states alone: the corn-soybean agroecosystem [zea mays l.-glycine max (l.) merr.]. nitrogen (n) fertilization could influence the soil carbon (c) balance in this system because the corn phase is fertilized in 97-100% of farms, at an average rate of 135 kg n x ha(-1) x yr(-1). we evaluated the impacts on two major processes that determine the soil c balance, the rates of organic-carbon (oc) inputs and ...200919688919
[effects of enhanced solar uv-b radiation on the effective photosynthetic leaf area and milking phase of oat under natural field condition in qing tibetan plateau].stratospheric ozone depletion occurs mainly over polar regions during the spring when the solar ultraviolet b-band (280-315 nm, uv-b) radiation is most intense in a year, but over the qing tibetan plateau region, the intensity is highest from june to september when the amount of uv-b radiation reaching the regions is more than that in the adjacent areas lying in the same latitude by 10%. from june to september is just the time of plant's germination, development, and reproduction in the alpine r ...200919650494
conformational heterogeneity and propagation of structural changes in the lov2/jalpha domain from avena sativa phototropin 1 as recorded by temperature-dependent ftir spectroscopy.phototropins control phototropism, chloroplast movement, stomatal opening, and leaf expansion in plants. phototropin 1 (phot1) is composed of a kinase domain linked to two blue light-sensing domains, lov2 and lov1, which bind flavin mononucleotide. disruption of the interaction between the lov2 domain and a helical segment named jalpha, joining lov to the kinase domain, induces the subsequent kinase activity of phototropin 1 and further-downstream signal transduction. here we study the effects o ...200919580761
primary reactions of the lov2 domain of phototropin studied with ultrafast mid-infrared spectroscopy and quantum chemistry.phototropins, major blue-light receptors in plants, are sensitive to blue light through a pair of flavin mononucleotide (fmn)-binding light oxygen and voltage (lov) domains, lov1 and lov2. lov2 undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine and the fmn c(4a) atom. here, the primary reactions of avena sativa phototropin 1 lov2 (aslov2) were studied using ultrafast mid-infrared spectroscopy and quantum chemistry. the singlet excited state (s1) ...200919580760
changes in cadmium mobility during composting and after soil application.the effect of twelve weeks of composting on the mobility and bioavailability of cadmium in six composts containing sewage sludge, wood chips and grass was studied, along with the cadmium immobilization capacity of compost. two different soils were used and cd accumulation measured in above-ground oat biomass (avena sativa l.). increasing ph appears to be an important cause of the observed decreases in available cadmium through the composting process. a pot experiment was performed with two diffe ...200919398321
transformation of oats and its application to improving osmotic stress tolerance.oat (avena sativa l.), a worldwide temperate cereal crop, is deficient in tolerance to osmotic stress due to drought and/or salinity. to genetically transform the available commercial oat cultivars, a genotype-independent and efficient regeneration system from shoot apical meristems was developed using four oat cultivars: prairie, porter, ogle, and pacer. all these oat cultivars generated a genotype-independent in vitro differentiated multiple shoots from shoot apical meristems at a high frequen ...200919009445
new dart markers for oat provide enhanced map coverage and global germplasm characterization.genomic discovery in oat and its application to oat improvement have been hindered by a lack of genetic markers common to different genetic maps, and by the difficulty of conducting whole-genome analysis using high-throughput markers. this study was intended to develop, characterize, and apply a large set of oat genetic markers based on diversity array technology (dart).200919159465
high-resolution spatial and temporal analysis of phytoalexin production in oats.the production of oat (avena sativa l.) phytoalexins, avenanthramides, occurs in response to elicitor treatment with oligo-n-acetylchitooligosaccharides. in this study, avenanthramides production was investigated by techniques that provide high spatial and temporal resolution in order to clarify the process of phytoalexin production at the cellular level. the amount of avenanthramides accumulation in a single mesophyll cell was quantified by a combination of laser micro-sampling and low-diffuse ...200919148672
phytotoxic activity of salvia x jamensis.a study has been carried out on the surface exudate of salvia x jamensis, which showed a significant phytotoxic activity against papaver rhoeas l. and avena sativa l.. bioguided separation of the exudate yielded active fractions from which 3 beta-hydroxy-isopimaric acid (1), hautriwaic acid (2), betulinic acid (3), 7,8 beta-dihydrosalviacoccin (4), isopimaric acid (5), 14 alpha-hydroxy-isopimaric acid (7), 15,16-epoxy-7 alpha, 10 beta-dihydroxy-clerod-3,13(16),14-trien-17,12;18,19-diolide (8), c ...200920120094
evidence for variation in the optimal translation initiation complex: plant eif4b, eif4f, and eif(iso)4f differentially promote translation of mrnas.eukaryotic initiation factor (eif) 4b is known to interact with multiple initiation factors, mrna, rrna, and poly(a) binding protein (pabp). to gain a better understanding of the function of eif4b, the two isoforms from arabidopsis (arabidopsis thaliana) were expressed and analyzed using biophysical and biochemical methods. plant eif4b was found by ultracentrifugation and light scattering analysis to most likely be a monomer with an extended structure. an extended structure would facilitate the ...200919493973
in vitro fermentation of oat flours from typical and high beta-glucan oat lines.two publicly available oat (avena sativa) lines, "jim" and "paul" (5.17 and 5.31% beta-glucan, respectively), and one experimental oat line "n979" (7.70% beta-glucan), were used to study the effect of beta-glucan levels in oat flours during simulated in vitro digestion and fermentation with human fecal flora obtained from different individuals. the oat flours were digested by using human digestion enzymes and fermented by batch fermentation under anaerobic conditions for 24 h. the fermentation p ...200919572543
performance of growing lambs fed processed karanj (pongamia glabra) oil seed cake as partial protein supplement to soybean meal.the aim of this study was to determine whether processed karanj (pongamia glabra) oil seed cake can be used as a supplement to partially replace soybean meal (sbm). male lambs (n = 24) of uniform body weight (12.88 +/- 0.15 kg) were equally allotted at random to a sbm-based control (con) and three test concentrate mixtures, containing detoxified solvent extracted karanj cake (skc) using three processing methods: water washing (ww), 2.5% lime (lm) and 0.4% binder (bn) treatment. the processed skc ...200919320936
an extract from wild green oat improves rat behaviour.an extract of wild green oat (avena sativa l.), was tested in vivo in rats for its behavioural effects after chronic oral administration via extract-admixed food. thirty six male sprague-dawley rats received (a) standard diet (controls), (b) 10 g/kg extract-admixed food or (c) 100 g/kg extract-admixed food. the following behavioural tests were performed: elevated plus maze, forced swimming, conditioned avoidance response and tetradic encounter. body weight, food and fluid consumption were measur ...200919322760
coularray electrochemical evaluation of tocopherol and tocotrienol isomers in barley, oat and spelt grains.hexane extracts obtained from hordeum vulgare l. (barley), avena sativa l. (oat), triticum spelta schrank and triticum dicoccum schrank ex schübler (spelt or emmer) whole grains, were examined for their tocochromanol (tocopherol and tocotrienol) content. the analyses were carried out on fatty extracts by means of hplc coupled with a coulometric array electrochemical detector (ecd). due to the specific high selectivity of the detector, the sample can be directly injected without any preliminary t ...200919370933
alpaca plant poisonings: nitrate-nitrite and possible cyanide.nitrate-nitrite poisoning killed four adult alpacas and induced the abortion of a full-term fetus after access to oaten hay (avena sativa) containing 3.2% kno(3) equivalent in dry matter. necropsy findings were cyanosis, dark-coloured blood, and pulmonary congestion and oedema. aqueous humour from two adults contained 25 mg no(3)/l and that from the fetus contained 10 mg no(3)/l. cyanide poisoning possibly killed two adult wether alpacas that ate a garden-cultivated variety of osteospermum ecklo ...200919245625
intercropped oats (avena sativa) - common vetch (vicia sativa) silage in the dry season for small-scale dairy systems in the highlands of central mexico.small-scale dairy systems in the highlands of central mexico require feeding strategies based on quality home-grown forage that may reduce high concentrate costs. eight holstein cows paired by parity and date of calving were used in a split-plot experiment to evaluate supplementing 6 kg dm/cow/d of oat-vetch silage (ovs) in comparison to maize silage (ms) as dry season feeding, for a more intensive use of the land through an oat-vetch catch crop. cows had 9 h/d access to continuous grazing of pe ...200919002599
mapping of sts markers obtained from oat resistance gene analog sequences.two previously isolated resistance gene analogs (rgas) of oat have been located as rflps in the reference map of avena byzantina 'kanota' x avena sativa 'ogle' in regions either homologous or homoeologous to loci for resistance to puccinia coronata, the causal agent of crown rust. in this study, the rgas were mapped in two recombinant inbred line (ril) populations that segregate for crown rust resistance: the diploid avena strigosa x avena wiestii ril population (asw), which has been used for ma ...200919767892
computer analysis of phytochrome sequences from five species: implications for the mechanism of action.the amino acid sequences of phytochrome from avena sativa, oryza sativa, curcurbita pepo, pisum sativum and arabidopsis thaliana have been analyzed with a variety of computer programs, with a view to identifying areas of the protein which contribute to the properties of this photoreceptor. a region at the c-terminus has been shown to be amphiphilic, and by analogy with surface-seeking peptides, may be responsible for interaction of phytochrome with lipid bilayers. possible targeting sequences in ...20092291773
quantitative trait loci in the ogle/tam o-301 oat mapping population controlling resistance to puccinia coronata in the field.crown rust is the most damaging disease of cultivated oat (avena sativa) and genetic resistance is the primary means of controlling the disease. quantitative trait loci (qtl) with major and minor effects have been identified in ogle1040 and tam o-301 (most notably, pc58 and pcnqmg/lgcg from tam o-301 and ot-27 from ogle1040) through single-isolate greenhouse and field tests. to map loci and determine the effectiveness of previously identified qtl against naturally occurring pathogen populations ...201020373970
development and characterization of an oat tilling-population and identification of mutations in lignin and beta-glucan biosynthesis genes.oat, avena sativa is the sixth most important cereal in the world. presently oat is mostly used as feed for animals. however, oat also has special properties that make it beneficial for human consumption and has seen a growing importance as a food crop in recent decades. increased demand for novel oat products has also put pressure on oat breeders to produce new oat varieties with specific properties such as increased or improved beta-glucan-, antioxidant- and omega-3 fatty acid levels, as well ...201020459868
oats (avena sativa) reduce atherogenesis in ldl-receptor-deficient mice.the cholesterol-lowering properties of oats, largely ascribed to its contents of soluble fibers, beta-glucans, are well established, whereas effects on atherogenesis are less well elucidated. oats also contains components with reported antioxidant and anti-inflammatory effects that may affect atherogenesis. in this work we examined effects of oat bran on plasma cholesterol, markers of inflammation, enos expression and development of atherosclerosis in ldl-receptor-deficient (ldlr(-/-)) mice.201020553794
antioxidant activities of aged oat vinegar in vitro and in mouse serum and liver.the present study focused on the antioxidant activities of aged oat (avena sativa l.) vinegar. the antioxidant activities of oat and vinegar have been proved by many previous research studies. it should be noted that oat vinegar, as a novel seasoning, has antioxidant activity.201020564418
oat (avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class i chitinase.extracts from different higher plants were screened for the ability to inhibit the growth of penicillium roqueforti, a major contaminating species in industrial food processing. oat (avena sativa) seed extracts exhibited a high degree of antifungal activity and could be used directly on rye bread to prevent the formation of p. roqueforti colonies. proteins in the oat seed extracts were fractionated by column chromatography and proteins in fractions containing antifungal activity were identified ...201019224400
fall growth, nutritive value, and estimation of total digestible nutrients for cereal-grain forages in the north-central united states.throughout the southern great plains, wheat is managed frequently as a dual-purpose crop, but this production paradigm is not necessarily applicable throughout other regions of the united states, and a wider array of management options can be considered for forage-only uses of cereal grains. our objectives were to assess the fall-growth potential of wheat (triticum aestivum l.), triticale (x triticosecale wittmack), and oat (avena sativa l.) cultivars in wisconsin, and then to further evaluate a ...201019749014
bacterial stimulation of copper phytoaccumulation by bioaugmentation with rhizosphere bacteria.copper contaminated areas pose environmental health risk to living organisms. remediation processes are thus required for both crop production and industrial activities. this study employed bioaugmentation with copper resistant bacteria to improve phytoremediation of vineyard soils and copper mining waste contaminated with high copper concentrations. oatmeal plant (avena sativa l.) was used for copper phytoextraction. three copper resistant bacterial isolates from oatmeal rhizosphere (pseudomona ...201020937516
influence of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina.three studies were conducted to determine the effect of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina. in all three studies, samples of wireworm populations were taken from the soil by using oat, avena sativa l., baits. treatments were defined by the previous year's crop and were chosen to reflect common crop rotations in the region. across all three studies, eight wireworm species were recovered from the baits: conoderus amplicollis (gyllen ...201021309229
lipid asymmetry in plant plasma membranes: phosphate deficiency-induced phospholipid replacement is restricted to the cytosolic leaflet.as in other eukaryotes, plant plasma membranes contain sphingolipids, phospholipids, and free sterols. in addition, plant plasma membranes also contain sterol derivatives and usually <5 mol% of a galactolipid, digalactosyldiacylglycerol (dgdg). we earlier reported that compared to fully fertilized oats (avena sativa), oats cultivated without phosphate replaced up to 70 mol% of the root plasma membrane phospholipids with dgdg. here, we investigated the implications of a high dgdg content on membr ...201019966136
drought stress induced changes in lipid peroxidation and antioxidant system in genus avena.seven species of genus avena viz., avena sativa, avena strigosa, avena brevis, avena vaviloviana, avena abyssinica, avena marocana and avena sterilis were used to study the impact of drought stress on lipid peroxidation and other antioxidant enzymes. maximum increase in the catalase activity was recorded in a. vaviloviana (129.97%) followed by a. sativa (122.82%) and a. brevis (83.38%) at vegetative stage; however at flowering stage the maximum increase was reported in a. sativa (25.62%) followe ...201021186716
mechanism of signal transduction of the lov2-jα photosensor from avena sativa.fusion proteins containing blue-light-activable protein domains possess great potential as molecular switches in cell signalling. this has recently been impressively demonstrated by connecting the light oxygen voltage lov2-jα-protein domain of a. sativa (aslov2-jα) with the rac1-gtpase, responsible for regulating the morphology and motility of metazoan cells. however, a target-oriented development of fusion proteins in conjunction with this photosensor is still very challenging, because a detail ...201021081920
increased water resistance of ctmp fibers by oat (avena sativa l.) husk lignin.the insertion of oat husk lignin onto chemithermomechanical pulp (ctmp) fibers was studied to increase fiber hydrophobicity. the pretreated pulp samples were subsequently used for preparation of handsheets for characterization. treatment of ctmp with laccase in the presence of oat husk lignin resulted in a significant increase in hydrophobicity of the handsheet surface, as indicated by dynamic contact angle analysis. water absorption time of 8 s was obtained with initial contact angle of 118°. a ...201020973545
electrogenic plasma membrane h+-atpase activity using voltage sensitive dyes.fast responding voltage sensitive dyes, rh421 and di-4-aspbs, were used to study the electrogenic properties of plant plasma membrane proton pumps on sealed plasma membrane vesicles extracted by two-phase partitioning from beta vulgaris and avena sativa cv swan root material. fluorescence spectroscopy in the presence of the dye rh421 (10.8 nm) was sufficiently sensitive to detect electrogenic activity of the extracted plant vesicles. the dye detection system could detect inhibition of electrogen ...201020734224
soluble sugar availability of aerobically germinated barley, oat and rice coleoptiles in anoxia.physiological and metabolic responses to anoxia were compared for aerobically germinated seedlings of barley (hordeum vulgare), oat (avena sativa) and rice (oryza sativa). coleoptile growth of barley, oat and rice seedlings was suppressed by a 24 h-anoxic stress, but the growth of the rice coleoptiles was much greater than that of the barley and oat coleoptiles. atp concentration in the anoxic rice coleoptiles was greater than that in the anoxic barley and oat coleoptiles. concentrations of etha ...201020727618
in situ disappearance of dry matter and fiber from fall-grown cereal-grain forages from the north-central united states.recent research has demonstrated that fall-grown wheat (triticum aestivum l.), triticale (x triticosecale wittmack), and oat (avena sativa l.) forages contain limited lignin and exhibit relatively stable estimates of in vitro true dm disappearance, as well as tdn, across a wide fall-harvest window. these traits suggest that ruminal availability of forage fiber is extensive. our objectives were to evaluate this premise by assessing in situ dm and ndf disappearance for kaskaskia wheat, trical 2700 ...201020709871
a new chromosome nomenclature system for oat (avena sativa l. and a. byzantina c. koch) based on fish analysis of monosomic lines.fluorescent in situ hybridization (fish) with multiple probes was used to analyze mitotic and meiotic chromosome spreads of avena sativa cv 'sun ii' monosomic lines, and of a. byzantina cv 'kanota' monosomic lines from spontaneous haploids. the probes used were a. strigosa pas120a (a repetitive sequence abundant in a-genome chromatin), a. murphyi pam1 (a repetitive sequence abundant in c-genome chromatin), a. strigosa pits (internal transcribed spacer of rdna) and the wheat rdna probes pta71 (nu ...201020658121
heavy metal concentrations in plants and different harvestable parts: a soil-plant equilibrium model.a mathematical interaction model, validated by experimental results, was developed to modeling the metal uptake by plants and induced growth decrease, by knowing metal in soils. the model relates the dynamics of the uptake of metals from soil to plants. also, two types of relationships are tested: total and available metal content. the model successfully fitted the experimental data and made it possible to predict the threshold values of total mortality with a satisfactory approach. data are tak ...201020605666
use of earthworms (eisenia fetida) to reduce phytotoxicity and promote humification of pre-composted olive oil mill wastewater.olive mill wastewaters (omww) contain a high recalcitrant organic load and an associated toxicity that make their treatment necessary before environmental application. the aim of this study was to evaluate the feasibility of promoting the valorization and reducing the phytotoxicity of omww through a pre-composting process together with straw-chip bulking materials followed by the application of earthworms (eisenia fetida) in the presence of oat seedlings (avena sativa l.) seedlings.201020602513
rationally improving lov domain-based photoswitches.genetically encoded protein photosensors are promising tools for engineering optical control of cellular behavior; we are only beginning to understand how to couple these light detectors to effectors of choice. here we report a method that increases the dynamic range of an artificial photoswitch based on the lov2 domain of avena sativa phototropin 1 (aslov2). this approach can potentially be used to improve many aslov2-based photoswitches.201020562867
qtls for important breeding characteristics in the doubled haploid oat progeny.a homozygous mapping population, consisting of doubled haploid (dh) oat (avena sativa l.) plants generated through anther culture of f1 plants from the cross between the finnish cultivar 'aslak' and the swedish cultivar 'matilda', was used to construct an oat linkage map. ten agronomic and quality traits were analyzed in the dh plants from field trials in 2005 and 2006. leaf blotch (caused by pyrenophora avenae) resistance was also evaluated in a greenhouse test with 2 different isolates. one to ...201020555437
development of oat-based markers from barley and wheat microsatellites.although microsatellites are an efficient and reliable genetic marker system, availability is limited in cultivated oat (avena sativa l.). previous research has suggested that microsatellites from related species may be adapted to oat. this study investigated the stability of existing oat microsatellites, sequenced polymorphic oat amplicons derived from wheat (triticum aestivum l.) and barley (hordeum vulgare l.) primers, and redesigned primers to develop oat-based markers. we evaluated 161 publ ...201020555435
effects of native grass cover crops on beneficial and pest invertebrates in australian vineyards.indigenous cover crops have the potential to promote an increase in natural enemies providing fortuitous control of pest species and other ecosystem services. we test this idea in a vineyard in south eastern australia, where reduced water availability because of drought coupled with increased temperatures has generated interest in sustainable alternatives to the exotic perennial cover crops commonly planted. three endemic perennial cover crops, comprising the grasses austrodanthonia richardsonii ...201020550812
hindered rotation of a cofactor methyl group as a probe for protein-cofactor interaction.exploring protein-cofactor interactions on a molecular level is one of the major challenges in modern biophysics. based on structural data alone it is rarely possible to identify how subtle interactions between a protein and its cofactor modulate the protein's reactivity. in the case of enzymatic processes in which paramagnetic molecules play a certain role, epr and related methods such as endor are suitable techniques to unravel such important details. in this contribution, we describe how cryo ...201020536240
mobilization of lipid reserves during germination of oat (avena sativa l.), a cereal rich in endosperm oil.since the cereal endosperm is a dead tissue in the mature grain, beta-oxidation is not possible there. this raises the question about the use of the endosperm oil in cereal grains during germination. in this study, mobilization of lipids in different tissues of germinating oat grains was analysed using thin-layer and gas chromatography. the data imply that the oat endosperm oil [triacylglycerol (tag)] is not a dead-end product as it was absorbed by the scutellum, either as free fatty acids (ffas ...201020497973
variability in xylanase and xylanase inhibition activities in different cereals in the healthgrain diversity screen and contribution of environment and genotype to this variability in common wheat.endo-1,4-beta-d-xylanases (ec 3.2.1.8, xylanases) and xylanase inhibitors, that is, taxi (triticum aestivum xylanase inhibitor), xip (xylanase inhibiting protein), and tlxi (thaumatin-like xylanase inhibitor) type xylanase inhibitors, which naturally occur in cereals, are believed to be at the basis of a significant part of the variability in biotechnological functional properties of cereals. xylanase inhibitors in particular affect grain functionality during processing and in animal feeds when ...201020462210
the chromosome content and genotype of two wheat cell lines and of their somatic fusion product with oat.somatic hybridization seeks to genetically combine phylogenetically distant parents. an effective system has been established in bread wheat (triticum aestivum l.) involving protoplasts from a non-totipotent cell line adapted to in vitro culture (t(1)) in combination with totipotent protoplasts harvested from embryogenic calli (t(2)). here, we report the karyotype and genotype of t(1) and t(2). line t(1) carries nine a (a-genome of wheat), seven b (b-genome of wheat) and eight d (d-genome of whe ...201020204401
population structure and linkage disequilibrium in oat (avena sativa l.): implications for genome-wide association studies.the level of population structure and the extent of linkage disequilibrium (ld) can have large impacts on the power, resolution, and design of genome-wide association studies (gwas) in plants. until recently, the topics of ld and population structure have not been explored in oat due to the lack of a high-throughput, high-density marker system. the objectives of this research were to survey the level of population structure and the extent of ld in oat germplasm and determine their implications f ...201121042793
210pb and 210po in finnish cereals.a survey was carried out on the activity concentrations of (210)pb and (210)po in cereal grains produced in finland. the cereal species were wheat (triticum aestivum), rye (secale cereale), oats (avena sativa) and barley (hordeum vulgare), which account for 90% of the finnish consumption of cereal products. the survey consisted of 18 flour and 13 unprocessed cereal samples and one hulled grain sample from 22 flour mills. according to the results, the mean (210)pb/(210)po concentrations in wheat ...201121035236
phytotoxic clerodane diterpenes from salvia miniata fernald (lamiaceae).our ongoing research to identify natural growth inhibitors with diterpene and triterpene skeletons exuding from the surface of the aerial parts of salvia species led us to study salvia miniata fernald. ten clerodane diterpenoids were found, along with three known diterpenes. most of the isolated compounds from s. miniata inhibited the germination of papaver rhoeas l. and avena sativa l. in petri dish experiments. parallel results have been obtained in experiments carried out to evaluate the subs ...201121130478
[photosystem ii characteristics of nine gramineae species in southern taklamakan desert].taking the gramineae species elytrigia intermedia, avena sativa, bromus inermis, elymus sibiricus, leymus tianschanicus, elymus dahuricus, festuca elata, agropyron cristatum, and puccinellia distans at the edge of cele oasis in southern taklimakan desert as test objects, this paper monitored their fast chlorophyll fluorescence kinetics after 20 minutes adaptation in darkness, compared their photosystem ii (ps ii) characteristics, and analyzed their adaptability to the local environment. among th ...201122263463
rhealba® oat plantlet extract: evidence of protein-free content and assessment of regulatory activity on immune inflammatory mediators.owing to their high content of flavonoids and saponins, plantlets of avena sativa l. (poaceae) are likely to possess anti-inflammatory and immunoregulatory properties of value in the treatment of atopic dermatitis (ad). with a view to its potential use in atopic subjects at risk of developing sensitisation to dietary proteins, we prepared a plantlet extract without proteins and isolated 2 flavonoids, isoorientin-2''- o-arabinoside (1) and isovitexin-2''- o-arabinoside (2), and two saponins, aven ...201121240840
allelopathic effects of ragweed (ambrosia artemisiifolia l.) on cultivated plants.during the past years ragweed has been coming to the forefront of interest in hungary and in other european countries as well because its serious health risk. results of the 5th national weed survey has proven that ragweed is the most important weed species on hungarian field lands, its coverage shows a rising tendency in cereals moreover it not only occurs in cultivated plants. allelopathic effects of aqueous extracts derived from different parts of ragweed plants (air dried leafy stems, seeds) ...201122696964
transferability and utility of white oat (avena sativa) microsatellite markers for genetic studies in black oat (avena strigosa).preservation and use of wild oat species germplasm are essential for further improvement of cultivated oats. we analyzed the transferability and utility of cultivated (white) oat avena sativa (aaccdd genome) microsatellite markers for genetic studies of black oat a. strigosa (a(s)a(s) genome) genotypes. the dna of each black oat genotype was extracted from young leaves and amplified by pcr using 24 microsatellite primers developed from white oat. the pcr products were separated on 3% agarose gel ...201122179963
effect of sewage water on mineral nutritive potential of six fodder species grown under semiarid conditions.investigation was performed to assess the effect of different sewage water treatments on the metal status of different fodder species consumed by ruminants under semi-arid conditions. five samples each of six fodder species viz., trifolium alexandrinum, cichorium intybus, avena sativa, medicago polymorpha, brassica campestris and medicago sativa were collected from three fields irrigated with canal water, mix water (canal water and sewage water) and sewage water, respectively. fodder samples wer ...201123961142
toxicity of sodium tungstate to earthworm, oat, radish, and lettuce.due to unknown effects of the potential exposure of the terrestrial environment to tungsten substances, a series of toxicity studies of sodium tungstate (na(2) wo(4) ) was conducted. the effect on earthworm (eisenia fetida) survival and reproduction was examined using organisation for economic co-operation and development (oecd) guideline 222. no effect on either endpoint was seen at the highest concentration tested, resulting in a 56-d no-observed-effect concentration (noec) of ≥586 mg tungsten ...201121805499
new diversity arrays technology (dart) markers for tetraploid oat (avena magna murphy et terrell) provide the first complete oat linkage map and markers linked to domestication genes from hexaploid a. sativa l.nutritional benefits of cultivated oat (avena sativa l., 2n = 6x = 42, aaccdd) are well recognized; however, seed protein levels are modest and resources for genetic improvement are scarce. the wild tetraploid, a. magna murphy et terrell (syn a. maroccana gdgr., 2n = 4x = 28, ccdd), which contains approximately 31% seed protein, was hybridized with cultivated oat to produce a domesticated a. magna. wild and cultivated accessions were crossed to generate a recombinant inbred line (ril) population ...201121805339
acute effects of an avena sativa herb extract on responses to the stroop color-word test.extracts from oat (avena sativa) herb may benefit cognitive performance. this study investigated whether neuravena(®), an oat herb extract, could acutely improve responses to the stroop color-word test, a measure of attention and concentration and the ability to maintain task focus.201121711204
effect of chemical systemic acquired resistance elicitors on avenanthramide biosynthesis in oat (avena sativa).oats produce a group of phenolic antioxidants termed avenanthramides. these metabolites are, among food crops, unique to oats and have shown, in experimental systems, certain desirable nutritional characteristics such as inhibiting atherosclerotic plaque formation and reducing inflammation. avenanthramides occur in both the leaves and grain of oat. in the leaves they are expressed as phytoalexins in response to crown rust (puccina coronata) infection. the experiments reported here demonstrate th ...201121598950
recent studies on aphrodisiac herbs for the management of male sexual dysfunction--a review.an aphrodisiac is a type of food or drink that has the effect of making those who eat or drink it more aroused in a sexual way. aphrodisiacs can be categorized according to their mode of action into three groups: substances that increase libido (i.e., sexual desire, arousal), substances that increase sexual potency (i.e., effectiveness of erection) and substances that increase sexual pleasure. some well-known aphrodisiacs are tribulus terrestrins, withania somnifera, eurycoma longifolia, avena s ...201121485695
evaluation of factors contributing to excessive nitrate accumulation in fodder crops leading to ill-health in dairy animals.a study was conducted to estimate nitrate content in commonly used fodder crops, viz., berseem (trifolium alexandrinum), bajra (pennisetum glaucum), maize (zea mays), oats (avena sativa), sorghum (sorghum vulgare) and toriya (brassica napus), collected from the fields of different villages of punjab and farms of the university, and to evaluate the factors associated with nitrate accumulation in these crops. the nitrate level was highest in sorghum on dry matter basis, followed by oats and toriya ...201121430916
modulating lov domain photodynamics with a residue alteration outside the chromophore binding site.phototropins, a class of light-activated protein kinases, are essential for several blue light responses in plants and algae, including phototropism. these proteins contain two internal light, oxygen, and voltage sensitive (lov) domains, which bind flavin chromophores and undergo a reversible photochemical formation of a cysteinyl-flavin adduct as part of the light sensing process. while the photodynamic properties of such photosensory domains are dictated by interactions between the chromophore ...201121323358
effects of water-saving superabsorbent polymer on antioxidant enzyme activities and lipid peroxidation in oat (avena sativa l.) under drought stress.drought stress significantly limits oat (avena sativa l.) growth and productivity. thus an efficient management of soil moisture and study of metabolic changes in response to drought are important for improved production of oat. the objective was to gain a better understanding of drought tolerance mechanisms and improve soil water management strategies using water-saving superabsorbent polymer (sap) at 60 kg ha(-1) under three irrigation levels (adequate, moderate and deficit) using a new type o ...201121302322
model snp development for complex genomes based on hexaploid oat using high-throughput 454 sequencing technology.genetic markers are pivotal to modern genomics research; however, discovery and genotyping of molecular markers in oat has been hindered by the size and complexity of the genome, and by a scarcity of sequence data. the purpose of this study was to generate oat expressed sequence tag (est) information, develop a bioinformatics pipeline for snp discovery, and establish a method for rapid, cost-effective, and straightforward genotyping of snp markers in complex polyploid genomes such as oat.201121272354
avenanthramides are bioavailable and accumulate in hepatic, cardiac, and skeletal muscle tissue following oral gavage in rats.avenanthramides (ava), polyphenols found exclusively in oats ( avena sativa l.), may play a role in the anti-inflammatory and antiatherogenic activity of oats. the bioavailability of ava has been demonstrated previously, but its distribution at the organ and tissue level and the extent of conjugation following ingestion have been unexplored. synthetic ava was administered to 24 rats by oral gavage, whereas 6 control rats received saline. ava concentrations were measured via hplc in plasma, liver ...201121417395
swine manure injection with low-disturbance applicator and cover crops reduce phosphorus losses.injection of liquid swine manure disturbs surface soil so that runoff from treated lands can transport sediment and nutrients to surface waters. we determined the effect of two manure application methods on p fate in a corn (zea mays l.)-soybean [glycine max (l.) merr.] production system, with and without a winter rye (secale cereale l.)-oat (avena sativa l.) cover crop. treatments included: (i) no manure; (ii) knife injection; and (iii) low-disturbance injection, each with and without the cover ...201121520739
dairy slurry application method impacts ammonia emission and nitrate in no-till corn silage.reducing ammonia (nh3) emissions through slurry incorporation or other soil management techniques may increase nitrate (no3) leaching, so quantifying potential losses from these alternative pathways is essential to improving slurry n management. slurry n losses, as nh3 or no3 were evaluated over 4 yr in south-central wisconsin. slurry (i.e., dairy cow [bos taurus] manure from a storage pit) was applied each spring at a single rate (-75 m3 ha(-1)) in one of three ways: surface broadcast (surf), s ...201121520745
ingested oat herb extract (avena sativa) changes eeg spectral frequencies in healthy subjects.abstract objective: this study aimed at using quantitative assessment of human electric brain activity during mental work for determining acute effects of ingested oat herb extract on cognitive performance. design: within a double-blind, randomized, placebo-controlled crossover study, two dosages of a special oat preparation of avena sativa herba (1250 or 2500 mg of neuravena(®)) were compared to placebo. an electroencephalogram was recorded while the patient had eyes open for 6 minutes, eyes cl ...201121563962
a peroxygenase pathway involved in the biosynthesis of epoxy fatty acids in oat (avena sativa l.).while oat has long been known to produce epoxy fatty acids in seeds, synthesized by a peroxygenase pathway, the gene encoding the peroxygenase remains to be determined. here we report identification of a peroxygenase cdna aspxg1 from developing seeds of oat. aspxg1 is a small protein with 249 amino acids in length and contains conserved heme-binding residues and a calcium-binding motif. when expressed in pichia pastoris and e. coli, aspxg1 catalyzes the strictly hydroperoxide-dependent epoxidati ...201121784965
characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat, and wheat.diazotrophic bacteria were isolated from the rhizosphere of field-grown triticum aestivum, hordeum vulgare, and avena sativa grown in various regions of greece. one isolate, with the highest nitrogen-fixation ability from each of the eleven rhizospheres, was selected for further characterisation. diazotrophic strains were assessed for plant-growth-promoting traits such as indoleacetic acid production and phosphate solubilisation. the phylogenies of 16s rrna gene of the selected isolates were com ...201121887633
comparative study the effects of solid-state fermentation with three filamentous fungi on the total phenolics content (tpc), flavonoids and antioxidant activities of subfractions from oats (avena sativa l.).the aim of present work was to investigate the effect of solid-state fermentation with filamentous fungi (aspergillus oryzae var. effuses, aspergillus oryzae, and aspergillus niger) on total phenolics content (tpc), flavonoids and antioxidant activities of four subfractions of oat, namely n-hexane, ethyl acetate (ea), n-butanol and water subfraction, and compare them with their corresponding subfractions of unfermented oat. the tpc and total flavonoids increased dramatically, especially in ea su ...201122136169
inhibition of fusarium graminearum growth in flour gel cultures by hexane-soluble compounds from oat (avena sativa l.) flour.fusarium head blight, incited by the fungus fusarium graminearum, primarily affects wheat (triticum aestivum) and barley (hordeum vulgarum), while oat (avena sativa) appears to be more resistant. although this has generally been attributed to the open panicle of oats, we hypothesized that a chemical component of oats might contribute to this resistance. to test this hypothesis, we created culture media made of wheat, barley, and oat flour gels (6 g of flour in 20 ml of water, gelled by autoclav ...201122186063
digital image sensor-based assessment of the status of oat (avena sativa l.) crops after frost damage.the aim of this paper is to classify the land covered with oat crops, and the quantification of frost damage on oats, while plants are still in the flowering stage. the images are taken by a digital colour camera ccd-based sensor. unsupervised classification methods are applied because the plants present different spectral signatures, depending on two main factors: illumination and the affected state. the colour space used in this application is cielab, based on the decomposition of the colour i ...201122163940
characterization of copper-resistant rhizosphere bacteria from avena sativa and plantago lanceolata for copper bioreduction and biosorption.copper is a toxic heavy metal widely used to microbial control especially in agriculture. consequently, high concentrations of copper residues remain in soils selecting copper-resistant organisms. in vineyards, copper is routinely used for fungi control. this work was undertaken to study copper resistance by rhizosphere microorganisms from two plants (avena sativa l. and plantago lanceolata l.) common in vineyard soils. eleven rhizosphere microorganisms were isolated, and four displayed high res ...201122002857
phytotreatment of sludges (phragmites australis) for their reuse in the environment.the aim of this study is the evaluation of the agronomic characteristics acquired by a phytotreated sludge coming from a wastewater treatment plant (wwtp) located in tuscany (central italy). the chemical characterization showed values which are within the italian legislation limits for mixed composts. from an agronomic point of view, the sludge did not present a level of phytotoxicity, as shown by the germination index (gi% = 77). furthermore, pathogen compounds are inexistent (escherichia coli ...201122214075
a formulation to encapsulate nootkatone for tick control.nootkatone is a component of grapefruit oil that is toxic to the disease-vectoring tick, ixodes scapularis say, but unfortunately causes phytotoxicity to treated plants and has a short residual activity due to volatility. we prepared a lignin-encapsulated nootkatone formulation to compare with a previously used emulsifiable formulation for volatility, plant phytotoxicity, and toxicity to unfed nymphs of i. scapularis. volatility of nootkatone was measured directly by trapping nootkatone vapor in ...201122238870
differential responses of oat genotypes: oxidative stress provoked by aluminum.the phytotoxic effects of aluminum and the mechanisms of genetically-based al tolerance have been widely investigated, as reported in many papers and reviews. however, investigations on many al-sensitive and al-resistant species demonstrate that al phytotoxicity and al-resistance mechanisms are extremely complex phenomena. the objective of the present study was to analyze the effects of aluminum on the activity of antioxidant enzymes such as catalase (cat), superoxide dismutase (sod), and ascorb ...201120853138
[primary structure of the "fast" component of avenin (avena sativa l].a procedure for the isolation and sequence analysis of the "fast" avenin component (n9) from the oat (avena sativa l., cv. narymsky 943) is described. component n9 was prepared by an ion-exchange high-performance liquid chromatography on a strong cation exchange column type mono s (pharmacia, sweden) in 4 m urea, ph 3.5, with a linear gradient of nacl. a polypeptide chain of avenin n9 was reconstructed by the cnbr and tryptic peptides on a model 470a protein gas-phase sequencer (applied biosyste ...20113190779
growth response of avena sativa in amino-acids-rich soils converted from phenol-contaminated soils by corynebacterium glutamicum.the biodegradation of phenol in laboratory-contaminated soil was investigated using the gram-positive soil bacterium corynebacterium glutamicum. this study showed that the phenol degradation caused by c. glutamicum was greatly enhanced by the addition of 1% yeast extract. from the toxicity test using daphnia magna, the soil did not exhibit any hazardous effects after the phenol was removed using c. glutamicum. additionally, the treatment of the phenolcontaminated soils with c. glutamicum increas ...201222534303
designing photoswitchable peptides using the aslov2 domain.photocontrol of functional peptides is a powerful tool for spatial and temporal control of cell signaling events. we show that the genetically encoded light-sensitive lov2 domain of avena sativa phototropin 1 (aslov2) can be used to reversibly photomodulate the affinity of peptides for their binding partners. sequence analysis and molecular modeling were used to embed two peptides into the jα helix of the aslov2 domain while maintaining aslov2 structure in the dark but allowing for binding to ef ...201222520757
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