Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| radionuclide contamination of foods imported into iraq following the chernobyl nuclear reactor accident. | since early 1986, a monitoring program for radionuclides in imported foods has been carried out by the iraqi atomic energy commission. after the chernobyl nuclear reactor accident in the soviet union, the program was expanded; our laboratory was officially designated by the iraqi government to measure radionuclide activity concentrations in foodstuff imported from countries known to be severely contaminated by chernobyl radioactive fallout. gamma-spectrometric analysis was used. food items such ... | 1991 | 1925528 |
| insulin sensitivity and liver insulin receptor structure in ducks from two genera. | insulin sensitivity and liver insulin receptor structure were studied in 5-wk-old ducks from two genera (muscovy and pekin). in the fasting state, both duck types were equally resistant to exogenous insulin compared with chicken. despite the low potency of duck insulin, the number of insulin receptors was lower in muscovy duck and similar in pekin duck and chicken liver membranes. after 125i-insulin cross-linking, the size of the alpha-subunit of the receptors from the three species was 135,000. ... | 1991 | 1928434 |
| dietary management of persistent diarrhea: comparison of a traditional rice-lentil based diet with soy formula. | recent studies have indicated that enteral diets can play an important role in the treatment of persistent diarrhea. khitchri, a local weaning food in pakistan, is composed of rice and lentils, which have previously been shown to be well tolerated in many children with acute diarrhea. the effectiveness of a khitchri and yogurt (ky) diet, which is inexpensive and widely available in pakistan, was studied. one hundred two weaned boys (6 to 36 months old) with persistent diarrhea were randomly assi ... | 1991 | 1945604 |
| increased hepatic insulin proreceptor-to-receptor ratio in diabetes: a possible processing defect. | hepatic insulin proreceptors and receptors were studied in control and in ketotic diabetic rats 2-4 wk after streptozotocin treatment. solubilized preparations were partially purified by wheat germ agglutinin-agarose (wga) and lentil lectin agarose (lla) chromatography to enrich eluates in insulin receptors and proreceptors, respectively. after phosphorylation with [gamma-32p]atp, an approximately 190-kda glycoprotein was identified in lla eluates as the insulin proreceptor, based on insulin dos ... | 1991 | 1951680 |
| glycoproteins in the retinal pigment epithelium of normal and dystrophic rats. | the apical membranes of retinal pigmented epithelium (rpe) were isolated from adult, normal (le), and dystrophic (rcs) rats. the proteins of these rpe subfractions were separated through the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the lectin-binding properties of glycoproteins were examined in western blots through the use of lectin-peroxidase conjugates. no differences were detected between rpe membrane proteins from normal and dystrophic rats in silver-stained gels. h ... | 1991 | 1993583 |
| isolation and characterization of a membrane glycoprotein from human brain with sequence similarities to cell adhesion proteins from chicken and mouse. | we previously described the production of monoclonal antibodies against a preparation of membrane glycoproteins from human brain [berglund et al. (1987) j. neurochem. 48, 809-815]. one of the glycoproteins, recognized by monoclonal antibody cf3, was specifically expressed in the brain. we now report the isolation and characterization of this glycoprotein, called glycoprotein 135 (gp135). gp135 was purified by means of lentil lectin affinity chromatography and immunoaffinity chromatography, using ... | 1991 | 2026173 |
| the collagen binding specificity of bone and platelet osteonectin is related to differences in glycosylation. | in this study we report that bone and platelet osteonectin are structurally and functionally heterogeneous in terms of glycosylation and collagen binding capacity. the relative sensitivity of bone and platelet osteonectin to specific glycosidases was used to evaluate potential differences in glycosylation. although native bone and platelet osteonectin are electrophoretically nonidentical, n-glycanase treatment yielded products with the same apparent molecular weight. bone osteonectin was also su ... | 1991 | 2033056 |
| on the multimeric nature of natural human interleukin-6. | natural human interleukin-6 (il-6) characterized under completely denaturing conditions consists of a set of differentially modified phosphoglycoproteins of molecular mass in the range from 23 to 30 kda ("25-kda" o-glycosylated species and "30-kda" o- and n-glycosylated species). the 25-kda o-glycosylated il-6 (which contains only ser- or thr-galnac-gal-neunac and thus should not bind wheat germ or lentil lectins) bound to and was eluted from a wheat germ lectin affinity column by glcnac and fro ... | 1991 | 2033081 |
| dietary plant materials and development of diabetes in the bb rat. | the present study was designed to examine further the impact of individual plant protein sources found in a diabetogenic, cereal-based, rodent laboratory diet, nih-07 [open formula, nonpurified rat and mouse diet (positive control)], on the development of diabetes. diabetes-prone bb rats that were pan-t(ox19+)-lymphopenic were fed a low diabetogenic diet during gestation and lactation. progeny of these rats were fed a normal or autoclaved nih-07 diet, or one of eight other diets based on the ain ... | 1991 | 2033475 |
| histochemical and immunohistochemical studies of diseased human glomeruli. | in order to study the localization of lentil lectin (lch)-binding glycoresidues in glomeruli from patients with a variety of glomerulopathies, and to elucidate the relationship between lch-binding sugars and the components of the extracellular matrix, laminin and type iv collagen, investigations of formalin-fixed, paraffin-embedded kidney tissues digested with trypsin were carried out by the direct and indirect immunofluorescence microscopy techniques. the glomerular basement membrane (gbm) and ... | 1991 | 2038128 |
| production and characterization of a mouse monoclonal antibody specific for lentil lectin. | a murine iga monoclonal antibody (moab) was produced against the widely used glucose/mannose-specific two-chain mitogen from lens culinaris (lentil) belonging to the vicieae tribe of the leguminosae family. the moab designated, 98, f-10, was found to be specific for lentil lectin when tested in dot blotting against 22 different native lectins. the antigenic specificity was also tested against subunits of 13 completely sequenced legume lectins separated by sodium dodecyl sulphate-polyacrylamide g ... | 1991 | 2039607 |
| structure of a new nervous system glycoprotein, nr-cam, and its relationship to subgroups of neural cell adhesion molecules. | we have identified and characterized a new glycoprotein in the chicken nervous system using immunological and molecular biological methods and we have examined its tissue distribution. analysis revealed that this protein is very similar in structure to the chicken neuron-glia cell adhesion molecule, ng-cam, and to mouse l1. cdna clones encompassing the entire coding sequence of this ng-cam related molecule, called nr-cam, have been isolated and sequenced. a glycoprotein containing one major comp ... | 1991 | 2045418 |
| carbohydrate composition of human megakaryocyte membranes in culture: identification using binding of seven lectins. | seven tetramethylrhodamine b isothiocyanate- (tritc) labeled lectins: lens culinaris (lch), ulex europeus-1 (uea-1), lycopersicon esculentum (lea), wheat germ agglutinin (wga), dolichos biflorus (dba), soybean agglutinin (sba) and erythrina cristagalli (eca) were applied on cultured human megakaryocytes (megs) detected by immunofluorescence. all stages of megs (from lymphocyte-like megs to mature megs) and platelets were labeled by lch, lea, uea-1 and wga. eca binds to platelets but only to some ... | 1991 | 2061623 |
| evidence for heterogeneity of glycosylation of human renin obtained by using lectins. | 1. in this study, the carbohydrate structure of pure human renin was examined by using various lectins. 2. pure renin could be separated into three forms by concanavalin a chromatography, a concanavalin a-unbound form, a loosely bound form and a tightly bound form, termed renins a, b and c, respectively. renins a, b and c accounted for 3, 13 and 84%, respectively, of the purified renin. these forms were all present in individual human plasma and the relative proportions in plasma were 27 +/- 3, ... | 1991 | 1655342 |
| presence of various carbohydrate moieties including beta-galactose and n-acetylglucosamine residues on solubilized porcine brain neurokinin-1/substance p receptors. | neurokinin-1 (nk-1)/substance p (sp) receptors were solubilized using 10 mm 3-[( cholamidopropyl)-dimethylammonio]-1- propanesulfate from porcine striatal membranes (solubilization yield, 80%). in solubilized preparations, [3h]sp apparently bound to a single class of high-affinity sites (kd = 0.82 +/- 0.13 nm) as in membrane homogenates. the ligand selectivity pattern observed in both membrane and solubilized receptor preparations indicated that [sar9,met(o2)11]sp = sp much greater than senktide ... | 1991 | 1658228 |
| immunogenicity in aujeszky's disease virus structural glycoprotein gvi (gp50) in swine. | aujeszky's disease virus (adv) envelope glycoprotein gvi (gp50) was purified from virus-infected vero cells by ion-exchange and immunoaffinity chromatography and its usefulness as a subunit vaccine was evaluated in active and passive immunization studies. four-week-old piglets were immunized intramuscularly (im) with purified gvi twice two weeks apart and challenged intranasally (in) 10 days after the second immunization with 30 ld50 (10(8)pfu) of a virulent strain of adv. pigs, vaccinated with ... | 1991 | 1660638 |
| molecular cloning of a human basic fibroblast growth factor receptor cdna and expression of a biologically active extracellular domain in a baculovirus system. | a cdna clone encoding a human fibroblast growth factor (fgf) receptor was isolated from a hepatoma cell line cdna library. the cdna encodes a three immunoglobulinlike-domain fgf receptor that is similar to a human placental fgf receptor cdna but lacks two amino acids. the variation observed at these two amino acids, also seen in the two immunoglobulinlike-domain fgf-receptors, can be explained by an alternate splicing mechanism. we have used a baculovirus expression system to produce high levels ... | 1991 | 1662973 |
| a role for microtubule bundles in the morphogenesis of chicken erythrocytes. | the acquisition of cellular asymmetry is one of the post-mitotic events in development. the cells of the avian erythropoietic lineage acquire a simple invariant asymmetry as they mature. erythrocytes develop in suspension from spherical through discoid to lentil-shaped (lentiform) cells, with a single rigorously specified microtubule bundle, the marginal band. we show here that developing erythrocytes can express highly asymmetric morphologies, including elongated cell bodies and long processes ... | 1991 | 1676841 |
| partial purification of the cystic fibrosis transmembrane conductance regulator. | we have investigated several purification strategies for the cystic fibrosis transmembrane regulator (cftr) based on its structural similarity to other proteins of the traffic atpase/abc transporter family. recombinant cftr expressed in heterologous cells was readily solubilized by digitonin and initially separated from the majority of other cellular proteins by sucrose density gradient centrifugation. cftr, with two predicted nucleotide binding domains, bound avidly to several triazine dye colu ... | 1992 | 1281484 |
| changes of soluble glycoproteins in dystrophic (dy/dy) mouse muscle shown by lectin binding. | lectin binding sites in skeletal muscle from normal and dystrophic (dy/dy) c57 bl/6j mice were demonstrated by use of histochemistry and electrophoresis combined with electron microscopy. the following lectins were used: canavalia ensiformis con a, triticum vulgaris (wga), glycine max (sba), griffonia simplicifolia (gs ii), arachis hypogaea (pna), pisum sativum (psa) and lens culinaris (lca). after incubation of frozen sections with con a, wga, gs ii, psa and lca a sarcoplasmic staining was obse ... | 1992 | 1290587 |
| antibodies to recombinant-derived glycoprotein b after natural human cytomegalovirus infection correlate with neutralizing activity. | glycoprotein b (gb) of human cytomegalovirus (hcmv) was partially purified by lentil-lectin column chromatography from cells infected with an adenovirus-gb recombinant. this antigen, which contained specifically reactive proteins of approximately 130 and 55 kda, was used to investigate gb antibody levels after natural hcmv infection in 48 individuals. all sera had igg antibody to gb as detected by radioimmunoprecipitation (rip) assays. quantitative rip showed a strong correlation between gb anti ... | 1992 | 1309850 |
| purification and characterization of human lymphoblast n-acetylglucosamine-1-phosphotransferase. | n-acetylglucosamine-1-phosphotransferase (glcnacptase) was solubilized with 2% tergitol np-10 from cultured human lymphoblast cells and purified 3840-fold with 14% recovery using lentil lectin-sepharose 4b, deae-sephacel and sephacryl s-400 chromatographies. the partially purified enzyme requires the non-ionic detergent tergitol np-10 and a divalent cation, mn2+ or mg2+, for its activity and exhibits an optimal ph at 7.2-7.5 in tris-maleate buffer. kinetic studies demonstrated an apparent km of ... | 1992 | 1318762 |
| incomplete digestion of legume starches in rats: a study of precooked flours containing retrograded and physically inaccessible starch fractions. | the digestibility of starch in precooked flours from green coat lentils (lens culinaris medik) and red kidney beans (phaseolus vulgaris l.) was investigated by balance experiments using rats treated with antibiotics to suppress hind-gut fermentation. the legume preparations were rich in intact cells filled with denaturated starch and contained retrograded amylose. between 8% (beans) and 11% (lentils) of the total starch ingested appeared in the feces, indicating a relatively low starch digestibi ... | 1992 | 1320113 |
| characterization of mineral-binding 40-kda glycoprotein extracted from young adult rabbit alveolar bone. | a forty-kilodalton (40-kda) protein was extracted from alveolar bone of young adult rabbit with 0.5 m edta after extraction with 4 m guhcl, and purified by gel-filtration, anion-exchange and hydroxyapatite columns using a high-pressure liquid chromatography system under denaturing conditions. the purified 40-kda protein was not susceptible to bacterial collagenase and thrombin, but was cleaved by cyanogen bromide. the protein was stained blue with stains-all. among various lectins, concanavalin ... | 1992 | 1323644 |
| oligosaccharide composition of the neurotoxin-responsive sodium channel of mouse neuroblastoma and requirement of sialic acid for biological activity. | a glycoprotein, m(r) 200,000, which has the biological activity of the neurotoxin-responsive na+ channel, was isolated from a clonal line of mouse neuroblastoma cells, n-18. the glycoprotein was purified to homogeneity in 18% yield by methods used to purify glycoproteins, which included metabolic labeling of the cells with l-[3h]fucose and binding of the radioactive glycoproteins to wga- and lentil-sepharose, and deae-cellulose. the glycoprotein has biological activity of neurotoxin-responsive i ... | 1992 | 1337866 |
| influence of different substrates in detoxification activity of adult rat hepatocytes in long-term culture: implications for transplantation. | substrates used to immobilize hepatocytes for transplantation govern attachment and long-term metabolic activity of these cells. the choice of these substrates is based on the familiarity with proteinaceous materials that are constituents of the extracellular matrix. the use of substrates that recognize carbohydrates on the cell surface may provide an alternative method to attach adult mammalian hepatocytes. in this study, immobilized lectins on tissue culture plasticware were used to support he ... | 1992 | 1344292 |
| impact of dietary protein and fat source on the development of insulin-dependent diabetes in the bb rat. | epidemiological studies show a remarkable geographical difference in the prevalence of iddm, suggesting a role for environmental factors such as diet, infection, or stress in the etiology of the disease. dietary modification has already been shown to be effective in the prevention of autoimmune diabetes in the bb rat and nod mouse. we studied the effect of protein and fat source in the prophylaxis of diabetes in the bb rat. natural ingredient rat chow was consistently associated with a high expr ... | 1992 | 1345000 |
| duck lymphocytes--v. transformation responses to phorbol ester and calcium ionophore. | 1. lymphocytes purified from duck blood and spleen were cultured in the presence of phorbol 12-myristate 13-acetate (pma) and the calcium ionophore a23187. stimulation was assessed by the incorporation of [3h]thymidine after 3 days' culture. 2. pma stimulated over a wide range of concentrations, with maximum stimulation at final concentrations of 5 x 10(-7)-5 x 10(-8) m/litre. a23187 was effective in the range 5 x 10(-6)-5 x 10(-7) m/litre and also, in some experiments using spleen lymphocytes, ... | 1992 | 1354147 |
| cd4-binding of gp130 micelles isolated from sivagmtyo-7. | the binding and binding inhibition of the sivagmtyo-7 external glycoprotein gp130 in micellar form to the cd4 molecule on human molt-4 clone 8 cells was investigated. the best binding of gp130 to molt-4 clone 8 cells occurred at ph 5.5 to 6.5 at 37 degrees c after 4 h or at room temperature after 10 h. the dissociation constant of this reaction was 0.2-0.4 nm, with both soluble cd4 or cd4 on molt-4 clone 8 cells. this value is close to 0.15 nm determined for the antihuman cd4 monoclonal antibody ... | 1992 | 1354467 |
| storage of glycoprotein in nctr-balb/c mouse. lectin histochemistry, and biochemical studies. | a strain of balb/c mice carrying a lysosomal storage disorder exhibits metabolic and phenotypic abnormalities similar to patients with sphingomyelin-cholesterol lipidoses type ii (i.e., niemann-pick c and d). their foamy cells, which belong to the reticuloendothelial system, stained intensely by periodate-schiff (pas) reagent and were resistant to predigestion with diastase. to identify the chemical nature of the pas-positive storage material, we applied lectin histochemistry and biochemical met ... | 1992 | 1360721 |
| purification of rat liver arylsulfatase a and its microheterogeneity assayed by crossed affinity-immunoelectrophoresis. | arylsulfatase a (arylsulfatase sulfohydrolase) ec 3.1.6.1 was purified from rat liver by a procedure consisting of differential centrifugation, con a-sepharose and blue sepharose chromatography, pbe 94 chromatofocusing, deae-cellulose and gel filtration chromatography followed by preparative electrophoresis. a molecular mass of 132,000 was estimated by gradient page. particular proteins were detected by immunoelectrophoresis. isoelectric focusing combined with immunoelectrophoresis gave two peak ... | 1992 | 1363453 |
| altered glycosylation of alpha-fetoprotein in hepadnavirus-induced hepatocellular carcinoma of the woodchuck. | altered glycosylation of alpha-fetoprotein (afp) has been proposed as a marker of hepatocellular carcinoma (hcc) in humans. the lectin-binding properties of woodchuck afp were investigated to determine if woodchuck hepatitis virus (whv)-induced hccs are also accompanied by changes in afp glycosylation. ninety-eight to 100% of the afp from normal, whv-free woodchucks with physiologic afp elevations and from whv-carrier woodchucks with hcc bound to concanavalin a, indicating that virtually all of ... | 1992 | 1373341 |
| establishment of a human hepatoblastoma model in athymic nude mice. producibility of fatty acid-binding protein, alpha-fetoprotein, and alpha 1-acid glycoprotein. | an athymic nude mouse model of human hepatoblastoma, designated hbl-2, was established. hbl-2 produced alpha-fetoprotein (afp) and the serum level in tumor-bearing mouse was roughly proportional to the tumor weight. reactivity of afp to concanavalin a and lens culinaris lectins indicated that it contained predominantly fucosylated, non-bisecting n-acetylglucosamine as a sugar moiety. alpha 1-acid glycoprotein (aag) was also found in both hbl-2 tumor extract and sera of tumor-bearing mice. the se ... | 1992 | 1376954 |
| glycosylation changes in human chorionic gonadotropin and free alpha subunit as gestation progresses. | carbohydrate is important to the structure, function, and circulatory survival of the glycoprotein hormones. human cg (hcg) and the related free alpha-molecule of pregnancy contain four and two asparagine-linked oligosaccharides, respectively. the present study analyzes changes in the glycosylation patterns of hcg and free alpha in early vs. late gestation. five volunteers provided 24-h urine samples, weekly, throughout their pregnancies. extracts of early pregnancy (weeks 7-12) and late pregnan ... | 1992 | 1377707 |
| interaction of lectins with human ige: ige-binding property and histamine-releasing activity of twelve plant lectins. | we examined the ige-binding reaction and the histamine-releasing response of basophils to a panel of 12 lectins: concanavalin a (con a), lens culinaris hemagglutinin (lch), pisum sativum agglutinin (psa), wheat germ agglutinin (wga), soybean agglutinin (sba), bauhinia purpurea agglutinin (bpa), peanut agglutinin (pna), ricinus communis agglutinin i (rca-i), lotus tetragonolobus agglutinin (lotus a), ulex europeus agglutinin i (uea-i), phytohemagglutinin e (pha-e) and phytohemagglutinin l (pha-l) ... | 1992 | 1378039 |
| growth of a human yolk sac tumor cell line with yolk sac-derived functions in selenium-supplemented chemically defined synthetic medium. | a human yolk sac tumor cell line, tg1, which was established from a testicular yolk sac tumor, was found to replicate continuously in a chemically defined medium supplemented with na2seo3 (isrpmi). tg1 produced several plasma proteins and growth factors: albumin, alpha-fetoprotein (afp), ferritin, carcinoembryonic antigen, beta-2-microglobulin, polyamine, neuron specific enolase, tissue polypeptide antigen, transferrin (tf), epidermal growth factor, and platelet derived growth factor. by analysi ... | 1992 | 1378830 |
| synthesis of 1-n-glycyl beta-oligosaccharide derivatives. reactivity of lens culinaris lectin with a fluorescent labeled streptavidin pseudoglycoprotein and immobilized neoglycolipid. | the lectin from lens culinaris (lentil) has a binding specificity for glycopeptides bearing 6-o-linked fucose on the reducing terminus on complex-type n-linked oligosaccharides. lentil lectin therefore provides an excellent example of a carbohydrate binding protein in which high-affinity interactions are dependent on the integrity of the oligosaccharide core structure. we report here the synthesis of the 1-n-glycyl beta-derivative of gal beta 4glcnac beta 2man alpha 6(gal beta 4glcnac beta 2man ... | 1992 | 1420189 |
| binding of thyrotropin to lentil lectin is unchanged by thyrotropin-releasing hormone administration in three patients with thyrotropin-producing pituitary adenomas. | glycoproteins have increased affinity for lentil lectin when fucose residues are bound to n-acetylglucosamine in the "core region" of their asparagine-linked oligosaccharides. in three patients with thyrotropin (tsh)-producing pituitary tumors, the proportion of serum tsh isoforms that bound to lentil (70.8% +/- 15%) was higher than that seen for tsh from normal persons (32.5 +/- 8%). unlike normal subjects, the concentration of tsh circulating in the tumor patients after acute administration of ... | 1992 | 1446658 |
| duck lymphocytes. vi. requirement for phagocytic and adherent cells in lymphocyte transformation. | preparations of duck (anas platyrhynchos) spleen and blood lymphocytes depleted of cells capable of phagocytosing carbonyl iron gave lower transformation responses to the mitogens phytohaemagglutinin (pha), concanavalin a (con a), bandeiraea simplicifolia seed lectin (bss), wheat germ agglutinin (wga), lentil lectin (ll) and phorbol ester (pma) than intact cell preparations. when cell populations were fractionated on the basis of their adherence to plastic, it was found that the adherent cells w ... | 1992 | 1455688 |
| ricinus communis agglutinin i reacting and non-reacting butyrylcholinesterase in human cerebrospinal fluid. | differences in glycosylation of acetylcholinesterase (ache) and butyrylcholinesterase (buche) in human brain, plasma and cerebrospinal fluid (csf) have been investigated by means of their interaction with agarose-immobilized lectins. most of the ache in brain and csf was associated to concanavalin a (con a), lens culinaris (lca) and triticum vulgaris (wga) agglutinins, but little activity was adsorbed to ricinus communis agglutinin i (rcai). brain, plasma and csf buche was almost fully bound to ... | 1992 | 1461569 |
| characterisation of a novel glycoprotein (avgp50) in the avian nervous system, with a monoclonal antibody. | a size fractionated lentil lectin-positive fraction derived from a deoxycholate extract of 1-day-old chick forebrain membranes was used to generate a series of monoclonal antibodies (mabs) against neural antigens. one of these, mabsa1.7 recognises a glycoprotein which is enriched in synaptic plasma membranes, designated avgp50. polyacrylamide gel electrophoresis and western blots show that avgp50 is comprised of at least two glycoforms, with m(r)s of 53 kda and 49 kda respectively. avgp50 is ner ... | 1992 | 1473276 |
| [a method for determining the presence of inverted repeats in the chloroplast genome of higher plants]. | a plasmid containing fragments of rp12 and rps19 genes from the chloroplast genome of arabidopsis thaliana was developed. the presence of inverted repeats in the chloroplast dna of a. thaliana and vicia sativa, and their absence from two species of fabaceae family (lathyrus sativus, lens esculenta) were shown with the help of this plasmid. | 1992 | 1473714 |
| mature domestic cat oocyte does not express a cortical granule-free domain. | a premature release of cortical granules (cg), found in the cortex of unfertilized oocytes, and the resulting formation of a cg-free domain (cgfd) over the metaphase ii spindle are associated with nuclear maturation in the hamster and mouse. the objectives of our study were to characterize and compare the distribution of cg in immature, in vitro-matured, and in vivo-matured domestic cat oocytes while determining if a cgfd is formed that may be useful as a marker for stage and normalcy of oocyte ... | 1992 | 1477197 |
| interaction of ache with lens culinaris agglutinin reveals differences in glycosylation of molecular forms in sarcoplasmic reticulum membrane subfractions. | fractionation of muscle microsomes rich in sarcoplasmic reticulum (sr) by isopicnic centrifugation yielded three types of membranes. heavy (hm), intermediate (im), and light membranes (lm), with isopicnic points of 38, 33, and 25% w/w sucrose, were rich in terminal cisternae/triads, longitudinal sr, and t-tubules, respectively. all membrane subfractions displayed acetylcholinesterase (ache) activity. about 60, 80, and 50% of total ache in hm, im, and lm was extracted with a tris-saline-triton bu ... | 1992 | 1484390 |
| fucosylation of glycoproteins begins in the rough endoplasmic reticulum of mouse active thyrotrophs. | our aim was to determine whether fucosylation of glycoproteins begins in the rough endoplasmic reticulum (rer) of active thyrotrophs. this would contrast with most cells studied, in which fucosylation generally is associated with the golgi apparatus. mouse thyrotropic tumor tissue was incubated with [35s]methionine for 2, 5, 7, 10, 30, and 90 minutes. tsh and free alpha-subunits were immunoprecipitated from cell lysates, and they displayed a time-dependent increase in affinity for lentil lectin ... | 1992 | 1493377 |
| interaction of dna with cationic liposomes: ability of transfecting lentil protoplasts. | the vesicle made of dipalmitoylphosphatidylcholine and stearylamine (9:1) were multilamellar and rather homogeneous in shape as seen by transmission electron microscopy. upon addition of circular dna plasmids of different lengths to the liposomes, the formation of vesicle clusters around the dna filament was observed, with dimensions dictated by the ratio dna/lipid. these liposomes were able to transfect lentil (lens culinaris) protoplasts inside the cells two different reporter genes, chloramph ... | 1992 | 1510671 |
| bronchial asthma induced by chick pea and lentil. | allergic reactions to legumes through inhalation have rarely been described. we report the case of a 20-year-old man who experienced asthmatic attacks when exposed to the steam from cooking either chick pea or lentil. type i hypersensitivity to the antigens in these legumes was demonstrated by means of immediate skin reactivity, histamine release tests, rast and rast inhibition. specific bronchial challenges with the heated (75 degrees for 30 min) extracts of chick pea and lentil elicited isolat ... | 1992 | 1514671 |
| determination of the carbohydrate-binding site of bauhinia purpurea lectin by affinity chromatography. | to determine the carbohydrate-binding site of bauhinia purpurea lectin (bpa), a d-galactose- and lactose-binding lectin, a peptide which interacts with lactose was purified from endoproteinase asp-n digests of bpa by chromatography on a lactose-sepharose column. it consists of nine amino acids and its amino acid sequence is asp-thr-trp-pro-asn-thr-glu-trp-ser. a tryptic fragment with the ability to interact with lactose was also purified and found to contain this sequence, consisting of nine ami ... | 1992 | 1517321 |
| the typing of staphylococcus epidermidis by a lectin-binding assay. | a new typing method for staphylococcus epidermidis was developed. four biotinylated lectins--wheat germ agglutinin (wga), soy bean agglutinin (sba), lentil agglutinin (lca) and concanavalin a (cona)--were added to immobilised whole cells of coagulase-negative staphylococci (cns) in microtitration plates. the amount of bound lectin was measured by peroxidase-conjugated avidin followed by a peroxidase reaction. the method was compared to antibiotic-resistance analysis, phage typing, plasmid dna pr ... | 1992 | 1518035 |
| ricin and lentil lectin-affinity chromatography reveals oligosaccharide heterogeneity of thyrotropin secreted by 12 human pituitary tumors. | some patients with thyrotropin (tsh)-producing pituitary tumors are more hyperthyroid than others despite similar tsh levels in serum, suggesting that qualitatively different tsh molecules with differing bioactivities may be secreted by different tumors. we used ricin and lentil lectin-affinity chromatography to test whether the tsh oligosaccharides varied among 12 patients with tsh-producing tumors. we found that each tumor secreted heterogeneous isoforms of tsh that differed in their extents o ... | 1992 | 1518416 |
| bioavailability and toxicological potential of lentil-bound residues of malathion in rats. | lentil grains treated with malathion and stored under laboratory conditions for 12 months formed bound residues. bioavailability and the effects of lentil-bound residues of malathion in rats were studied. the amount of bound residues in lentils treated with 14c-malathion at 10 ppm and 50 ppm gradually increased to 9.52% and 13.01% of the initially applied doses after 12 months. when rats were fed these 14c-bound residues, radioactivity excreted in urine accounted for 34.49% of the administered d ... | 1992 | 1527355 |
| sustained slowing effect of lentils on gastric emptying of solids in humans and dogs. | the distal small intestine is an especially potent site for carbohydrate-triggered intestinal inhibition of gastric emptying of solids. poorly digestible carbohydrates, such as lentils, may escape proximal absorption, travel over time to reach these inhibitory mechanisms, and slow the gastric emptying of a later meal. a slowing effect on gastric emptying may be associated with a lowering effect on postprandial glucose. the aims of this study were to determine (a) whether lentils (a poorly digest ... | 1992 | 1537516 |
| effect of lectins on hepatic clearance and killing of candida albicans by the isolated perfused mouse liver. | the isolated perfused mouse liver model was used to study the effects of various lectins on hepatic trapping and killing of candida albicans. after mouse livers were washed with 20 to 30 ml of perfusion buffer, 10(6) c. albicans cfu were infused into the livers. at the time of recovery, 63% +/- 2% (mean +/- standard error of the mean) of the infused c. albicans cfu were recovered from the liver and 14% +/- 1% were recovered from the effluent for a total recovery of 77% +/- 2%. this indicated tha ... | 1992 | 1541519 |
| intravenous thyrotropin (tsh)-releasing hormone releases human tsh that is structurally different from basal tsh. | to determine whether basal tsh differed structurally from trh-released tsh, the tsh obtained from 11 normal subjects before and after the iv administration of trh was characterized using lectin-affinity chromatography. tsh was applied to the following lectins: lentil, ricin (both before and after tsh treatment with neuraminidase), concanavalin-a, wheat germ, glycine max, helix pomatia, dolichos biflorus, arachis hypogaea, and vicia villosa (isolectin b4). after each column was washed to elute un ... | 1992 | 1592875 |
| alteration of the carbohydrate-binding specificity of the bauhinia purpurea lectin through the preparation of a chimeric lectin. | a chimeric lectin gene was constructed by using a cdna clone coding the bauhinia purpurea lectin (bpa) in which a part of the metal-binding region was replaced by the corresponding region of the mannose-binding lens culinaris lectin (lca). the chimeric lectin expressed in escherichia coli was found to bind alpha mannosyl-bovine serum albumin (bsa) and this binding was inhibited by mannose. | 1992 | 1607368 |
| influence of metribuzin on the rhizobium leguminosarum--lentil (lens culinaris) symbiosis. | the effects of the triazine herbicide metribuzin (sencor) on the lentil (lens culinaris medic.) - rhizobium leguminosarum biovar viciae symbiosis were studied in leonard jars and growth pouches. lentils inoculated with rhizobium leguminosarum strain 128c54 or 128c84, and noninoculated lentils grown in plant nutrient solution supplemented with 5 mm kno3, had metribuzin applied to the plants at either 8 or 13 days after planting. when sprayed at 8 days, metribuzin had a significant (p less than or ... | 1992 | 1611561 |
| glycosylation of membrane cofactor protein (cd46) in human trophoblast, kidney and platelets. | many cell surface glycoconjugates are differentiation markers and are involved in cell-cell and intermolecular interactions in development, immunity and cancer. membrane cofactor protein (mcp) comprises structurally related 65 and 55 kda glycoproteins that bear o- and n-linked glycans. mcp prevents amplification of autologous complement action on human cells. we used immunoblotting with mcp-specific monoclonal antibody tra-2-10 to determine lectin-binding properties and glycosidase sensitivities ... | 1992 | 1627608 |
| human skin mast cell carboxypeptidase: functional characterization, cdna cloning, and genealogy. | we functionally characterized human skin mast cell carboxypeptidase a (mc-cpa), and explored its evolutionary relationship to other carboxypeptidases to understand further the structural basis for the substrate preferences of this enzyme. purified human skin mc-cpa displayed more activity than did bovine pancreatic carboxypeptidase a (cpa) against carboxyl-terminal leucine residues, about equal activity with phenylalanine and tyrosine residues, and no activity with tryptophan or alanine. to corr ... | 1992 | 1629626 |
| histochemical analysis of rat testicular glycoconjugates. 1. subsets of n-linked saccharides in seminiferous tubules. | the distribution of n-linked glycans in rat testis has been probed using a panel of lectins derived from galanthus nivalis (snowdrop, gna), canavalia ensiformis (jack bean, con a), lens culinaris (lentil, lca), pisum sativum (garden pea, psa) and phaseolus vulgaris, erythro- and leucoagglutinins (kidney bean, epha and lpha). several classes of n-linked glycan were identified in the spermatogenic series, and during differentiation into spermatozoa they altered in both their pattern of distributio ... | 1992 | 1634371 |
| the use of streptavidin-biotinylglycans as a tool for characterization of oligosaccharide-binding specificity of lectin. | a new rapid and sensitive method for characterizing lectin specificity using streptavidin-biotinylglycans as a tool is presented. this assay is analogous to enzyme immunoassay and takes advantage of the strong, irreversible adsorption of streptavidin to the wells of the chambers of titer plates. a series of streptavidin-biotinylglycans was first coated on a microtiter plate, and then one of six lectins, concanavalin a, wheat germ agglutinin, phaseolus vulgaris (red kidney bean) erythro-agglutini ... | 1992 | 1443561 |
| alpha 1-6(alpha 1-3)-difucosylation of the asparagine-bound n-acetylglucosamine in honeybee venom phospholipase a2. | chymotryptic glycopeptides were prepared from a honeybee (apis mellifica) venom phospholipase a2 (e.c. 3.1.1.4) fraction, with high affinity towards lentil (lens culinaris) lectin. treatment of the glycopeptide mixture with peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase a, followed by hplc fractionation, yielded two oligosaccharides, which were analysed by 500 mhz 1h-nmr spectroscopy to give the following structures [formula: see text] this is the first report on a naturally occurring ... | 1992 | 1344712 |
| performance of chickpea, lentil and lupin nodulated with indigenous or inoculated rhizobia micropartners under nitrogen, boron, cobalt and molybdenum fertilization schedules. | chickpea (cicer arietinum), lentil (lens esculenta) and lupin (lupinus albus) responded to inoculation with their respective symbiotants:rhizobium loti, rhizobium leguminosarum biovarviceae andbradyrhizobium sp. (lupinus) alone or with (nh4)2so4 at 30 ppm n. soil application of na2moo4.2h2o at 3 ppm mo, cocl2.6h2o at 2 ppm co(2+) or na2b4o7.10h2o at 1 ppm b with 0 and 30 ppm n increased nodule weight and plant dry weight and n-content 60 days after sowing and seed yield, seed size and the n and ... | 1992 | 24425609 |
| agrobacterium tumefaciens-mediated beta-glucuronidase (gus) gene expression in lentil (lens culinaris medik.) tissues. | lentil (lens culinaris medik.) shoot apex, epicotyl, and root expiants were capable of expressing an intron-containing beta-glucuronidase (gus) gene after inoculation with the disarmed agrobacterium strain gv2260:p35sgusint. expression occurred at all wound sites on these expiants except at the end of the root expiants proximal to the cotyledonary node. gus expression was detected using both histochemical and fluorescence assays and was stable for at least nine days after inoculation for epicoty ... | 1992 | 24203139 |
| ultrastructural localization and semiquantitative analysis of glycoconjugates in the tectorial membrane. | the tectorial membrane of the gerbil cochlea was analyzed with lectin-gold cytochemical methods for demonstrating and characterizing glycoconjugates (gcs) in situ. binding of lectins from limax flavus (lfa), lens culinaris (lca), datura stramonium (dsa), ricinus communis (rca i), ulex europeus (uea i) and phaseolus vulgaris (pha l) was assayed semiquantitatively on ultrathin sections. binding occurred throughout the tectorial membrane with all lectins except uea i but the labelling density with ... | 1992 | 1559904 |
| factors influencing the reaction of alpha 1-fetoprotein with concanavalin a and lens culinaris agglutinin in crossed affinoimmunoelectrophoresis. | concanavalin a (con a) and lentil lectin (lca) analysis of alpha-fetoprotein (afp) glycosylation heterogeneity is used in a variety of clinical situations. we studied the influence of analytical conditions on the separation of afp glycoforms by using lectin-crossed affinoimmunoelectrophoresis, regardless of the afp concentration, which can vary over a wide range in biological fluids. we defined the optimal concentration of con a (2 g/l) and lca (0.35 g/l) in the first-dimension gel, together wit ... | 1992 | 1379524 |
| altered lectin binding sites in keratoconus corneas. | we investigated the glycoconjugates in frozen sections of keratoconus corneas, using a panel of 12 biotin- or fluorescein isothiocyanate-labeled lectins. no differences between the lectin binding sites of the epithelium, endothelium and descemet's membrane of normal and keratoconus corneas could be observed. however, in contrast to normal corneas, intense staining with peanut agglutinin (pna) could be detected at breaks in bowman's layer, in scar tissue and in the adjacent stroma. furthermore, i ... | 1992 | 1606835 |
| two crystal forms of the lentil lectin diffract to high resolution. | the legume lectins are an important class of polysaccharide-binding proteins with a wide range of biochemical and immunological applications. two high-resolution crystal forms are obtained for the lentil (lens culinaris) lectin: a monoclinic p21 and an orthorhombic p212121. the unit cell dimensions for the monoclinic form are a = 58.0 a, b = 56.0 a, c = 82.1 a, beta = 104.4 degrees, while for the orthorhombic form a = 56.4 a, b = 74.6 a, c = 124.9 a. the asymmetric unit contains one dimer in bot ... | 1992 | 1738165 |
| comparison of the n- and o-linked glycopeptides of lymph node cells from c57 bl/6 lpr/lpr and c57 bl/6 mice. | we have tested the hypothesis that some phenotypic characteristics of the lymphocytes from mice with lymphoproliferative disease (lpr) could be explained by abnormal glycosylation of membrane proteins. lymph node cells from normal c57 bl/6 and from c57 bl/lpr mice were labelled with tritiated sugars. membrane proteins were released with trypsin, then with pronase. after complete pronase digestion, glycopeptides were first separated on bio gel p-6 and then on con a-sepharose. fractions not bindin ... | 1992 | 1299347 |
| alpha-fetoprotein glycosylation is abnormal in some hepatocellular carcinoma, including white patients with a normal alpha-fetoprotein concentration. | lectin-affinity analyses with lens culinaris agglutinin (lca) and other lectins have demonstrated that the glycosylation of alpha-fetoprotein (afp) secreted by hepatocellular carcinomas (hcc) is frequently altered when the serum afp concentration is increased. to determine if afp lca-binding properties are altered in patients with hcc whose serum afp concentration is normal, the percentage of lca-binding afp in serum from white newborns, white normal adults, white patients with chronic hepatitis ... | 1993 | 7506988 |
| serum alpha-fetoprotein and lens culinaris agglutinin-reactive fraction of alpha-fetoprotein in patients with hepatocellular carcinoma. | a fraction of serum alpha-fetoprotein (afp) reactive with lens culinaris agglutinin (lca) was measured by affinity chromatography in serum samples from 102 patients with hepatocellular carcinoma (hcc) and 48 patients with chronic liver diseases without hcc. its usefulness as a marker of hcc was evaluated. the mean +/- sd percentage of this fraction in total afp was 3.10 +/- 3.17% in 48 patients with chronic liver diseases without hcc. when the cut-off level was set at 12.6% (mean + 3 sd), the se ... | 1993 | 7690873 |
| highly enhanced fucosylation of alpha-fetoprotein in patients with germ cell tumor. | we have recently reported that simultaneous determination of the fucosylation and glucosaminylation of alpha-fetoprotein (afp) was useful in the differential diagnosis of various liver diseases. here, we measured these indices in germ cell tumor and compared them with those in various diseases to reveal the characteristic feature of the carbohydrate moiety of afp in this tumor. | 1993 | 7686444 |
| glycan microheterogeneity of alpha 1-antitrypsin in serum and meconium from normal and cystic fibrosis patients by crossed immunoaffinoelectrophoresis with different lectins (con a, lca, wga). | in order to test whether abnormalities of glycosylation occur in cystic fibrosis (cf), the glycan microheterogeneity of alpha 1-antitrypsin (alpha 1-at) was studied in serum and meconium from normal individuals and patients with cystic fibrosis, by crossed immunoaffinoelectrophoresis (ciae) using free concanavalin a (con a), lens culinaris lectin (lca) and wheat germ agglutinin (wga). three main results emerged from this study: (1) modification of glycosylation in serum alpha 1-at from patients ... | 1993 | 8403434 |
| establishment of assay kits for the determination of microheterogeneities of alpha-fetoprotein using lectin-affinity electrophoresis. | diagnostic kits for determination of alpha-fetoprotein (afp) carbohydrate chain microheterogeneity were developed using lectin affinity electrophoresis with lens culinaris agglutinin-a (lca-a) and erythro-agglutinating phytohemagglutinin-e4 (pha-e4). separated afp bands by electrophoresis were detected with high sensitivity by antibody-affinity blotting and immunoenzymatic amplification. densitometry was used to apportion lectin reactive afps. the within-run s.d. for proportions of afp bands was ... | 1993 | 7680970 |
| inheritance and linkage relationships of morphological and isozyme loci in chickpea (cicer arietinum l.). | inheritance and linkage relationships of several morphological and isozyme loci are described in chickpea (cicer arietinum l.). segregation data obtained from several f2 families confirmed the previously observed mode of inheritance for most of the morphological loci. additional morphological markers in chickpea are also described. most of the isozyme loci studied showed codominant expression and fit expected mendelian segregation ratios. however, distorted ratios were also observed for some loc ... | 1993 | 24193588 |
| carbohydrate structures of human alpha-fetoprotein of patients with hepatocellular carcinoma: presence of fucosylated and non-fucosylated triantennary glycans. | chemical structures of the sugar chains of various human alpha-fetoprotein (afp) species with different affinity for concanavalin a (con a) and lens culinaris agglutinin (lca) were examined by pyridylamination of their oligosaccharides and stepwise exoglycosidase digestion. using reversed-phase and size-fractionation high performance liquid chromatography systems we identified six pyridylamino-sugar chains. the con a-reactive and lca-nonreactive species of afp from patients with hepatocellular c ... | 1993 | 7679920 |
| early recognition of hepatocellular carcinoma based on altered profiles of alpha-fetoprotein. | the sugar-chain structures of circulating alpha-fetoprotein in patients with hepatocellular carcinomas differ from those in patients with cirrhosis. we studied the reactivity of alpha-fetoprotein with two lectins, lens culinaris agglutinin a and erythroagglutinating phytohemagglutinin, to monitor the evolution of hepatocellular carcinoma in patients with cirrhosis. | 1993 | 7684823 |
| a collaborative study for the evaluation of lectin-reactive alpha-fetoproteins in early detection of hepatocellular carcinoma. | lectin-affinity electrophoretic separation of serum alpha-fetoprotein (afp) was carried out using afp differentiation kits, which used lens culinaris agglutinin-a (kit l) and erythroagglutinating phytohemagglutinin (kit p). separated afp bands were detected with a sensitive antibody-affinity blotting technique and determined quantitatively by densitometry, and the results were expressed as percentages of the intensity of total afp bands. sera from 424 patients with acute hepatitis, chronic hepat ... | 1993 | 7693340 |
| determination of broad bean stain virus serotypes by enzyme-linked immunosorbent assay. | enzyme-linked immunosorbent assay (elisa) was used to determine the degree of serological specificity of two serotypes of broad bean stain virus (bbsv) and their relationship to red clover mottle virus (rcmv). optimal conditions for the differentiation by elisa of the two viruses and the two bbsv serotypes were established. bbsv isolates from vicia sativa belonged to serotype i, those from pea plants (f1, kow 60) to serotype ii, and isolates from lens culinaris differed from these two serotypes. ... | 1993 | 7905241 |
| insoluble dietary fiber of grain food legumes and protein digestibility. | the present work aimed to verify the digestibility of cooked whole food grain legumes. samples of beans (phaseolus vulgaris and vigna sinensis), chickpeas (cicer arietinum) and lentils (lens culinaris) were used in the experiment. the interrelationship between the insoluble dietary fiber presented in the food grain legumes and the low protein digestibility was studied. the insoluble dietary fiber and the proteic nitrogen presented in the neutral detergent fiber (ndf) were determined. "in vivo" d ... | 1993 | 8002707 |
| glycosylation of human prolactin regulates hormone bioactivity and metabolic clearance. | to analyze the role of individual glycosylation pattern on prl biopotency, monomeric prolactin (prl), secreted by human prolactinoma cells in culture, was isolated by gel filtration and separated by affinity chromatography on concanavalin a-sepharose or lentil-agarose. these lectins allowed the isolation of prl glycoforms containing either biantennary, mannose-rich or fucosylated complex carbohydrate structures, respectively. endoglycosidase treatment and carbohydrate content of prl was found to ... | 1993 | 8144855 |
| higher plant mitochondria encode an homologue of the nuclear-encoded 30-kda subunit of bovine mitochondrial complex i. | we describe the structure and expression of a wheat mitochondrial gene, which codes for a subunit of mitochondrial nadh dehydrogenase. the deduced protein sequence has 70% similarity to the 30-kda subunit of bovine mitochondrial complex i and 65% similarity to the 31-kda subunit of neurospora crassa complex i, components of the iron-sulfur-protein fraction, both nuclear-encoded proteins. we named this wheat mitochondrial gene as nad9. the wheat nad9 gene is transcribed in a single mrna of 0.9 kb ... | 1993 | 8223639 |
| demonstration of feline and canine platelet glycoproteins by immuno- and lectin histochemistry. | canine and feline platelet cytocentrifuge preparations (ccps), cryostat and paraffin-embedded bone marrow sections were used in this study. we evaluated whether platelets, megakaryocytes and megakaryocyte precursor cells could be labelled by monoclonal antibodies (y2/51, clb-thromb/1, hpl1) against human platelet membrane glycoprotein gp iiia and the gp iib/iiia complex or by the following 10 biotinylated lectins: concanavalin a (con a), lens culinaris agglutinin (lca), pisum sativum agglutinin ... | 1993 | 8226111 |
| schistosoma mansoni: surface membrane isolation with lectin-coated beads. | lectins from lens culinaris and arachis hypogaea immobilized on polyacrylamide beads were used for selective isolation of glycosylated surface membrane domains of adult schistosoma mansoni worms, and the method was compared with the membrane isolation procedure developed with polycationic (affi-gel) beads. the lentil lectin proved to be suitable for interaction with surface membrane components: an increment in the specific activities of tegumental phosphohydrolases was observed in the bound frac ... | 1993 | 8231898 |
| metacyclogenesis of leishmania (viannia) braziliensis in vitro: evidence that lentil lectin is a marker of complement resistance and enhanced infectivity. | axenic culture in modified grace's medium was used to induce metacyclogenesis of leishmania (viannia) braziliensis in vitro. morphological characteristics, lectin agglutination profiles, susceptibility to complement lysis, and infectivity in vivo were compared between metacyclic promastigotes and promastigotes in mid-log phase growth. short, arrow-like promastigotes and round, oval promastigotes were defined as putative metacyclic forms on the basis of being highly motile and free swimming, with ... | 1993 | 8236409 |
| increase of mannose residues, as salmonella typhimurium-adhering factor, on the cecal mucosa of germ-free chickens infected with eimeria tenella. | to study increase of the salmonella population in the cecum of chickens infected with eimeria tenella, quantitative changes in mannose residues on the cecal mucosa were investigated. inhibition of s typhimurium adherence to the cecum by a 2% carbohydrate (d-mannose, d-galactose, l-fucose, alpha-methyl-d-glucoside) in phosphate-buffered saline solution was examined. only d-mannose had inhibitory effects. whereas, d-galactose had somewhat enhancing effects on adherence of s typhimurium to the ceca ... | 1993 | 8239135 |
| purification and characterization of sperm-coating antigen, identified by a monoclonal antibody. | a procedure was designed for purification of a human seminal plasma-specific antigen (hsp-antigen) identified by a monoclonal antibody produced in this laboratory (mab 4e6). pooled human seminal plasma was fractionated by consecutively applied methods: affinity chromatography on lentil lectin sepharose, gel chromatography on ultrogel aca 34 and immunoaffinity on mab 4e6 coupled cnbr-sepharose 4b. the antigen-containing fraction obtained after this procedure was proved to be homogeneous when anal ... | 1993 | 8250290 |
| 5-propyl-2-deoxyuridine induced interference with glycosylation in herpes simplex virus infected cells. nature of pdu-induced modifications of n-linked glycans. | in herpes simplex virus-infected (hsv) cells, the antiviral nucleoside analogue 5-n-propyl-2'-deoxyuridine (pdu) may, under certain circumstances, induce a pattern of interference with late steps in formation of n-linked glycans, resulting in increased availability of viral glycoproteins for neutralizing antibodies. the pdu-induced changes in n-linked glycans, released by pronase digestion of the hsv-specified glycoprotein gc-1, were investigated by using lectin affinity chromatography and bio-g ... | 1993 | 8382038 |
| structural analysis on the sugar chains of human alpha 1-antitrypsin: presence of fucosylated biantennary glycan in hepatocellular carcinoma. | chemical structures of the sugar chains of alpha 1-antitrypsin (aat) from patients with hepatocellular carcinoma (hcc) and from healthy individuals with a different affinity for lens culinaris agglutinin (lca) were examined by pyridylamination of their oligosaccharides and stepwise exoglycosidase digestion in combination with reversed-phase and size-fractionation high-performance liquid chromatography. we found that the lca-reactive species of aat from patients with hcc carried both the biantenn ... | 1993 | 8390218 |
| monoclonal antibody recognizes different quinone moieties in enzymes. | we produced monoclonal antibodies against the coenzyme pyrrolequinoline quinone (pqq). these antibodies were obtained by immunizing mice with pqq conjugated to a chemically modified polypeptide in order to induce a strong immune response. among the various antibodies obtained, one was found to bind (besides pqq and 6-hydroxydopamine conjugated to carrier proteins) several different quinoenzymes, namely lentil seedling and bovine serum diamine oxidases and methylamine dehydrogenase. this antibody ... | 1993 | 8390461 |
| immunisation of macaques with siv env recombinants: specificity of t cell and antibody responses and evaluation of protective efficacy. | macaques were immunised with lentil lectin purified recombinant sivmac (bk28) derived gp160 (rgp160) with or without live vaccinia (vac)-env (bk28) priming, followed by a final boost with solid matrix antibody antigen (smaa)-gp160 (j5) complexes and challenged with the sivmac molecularly cloned virus j5m. rgp160 and vac-env plus gp160 induced strong ab responses against the homologous virus. live vac-env did not enhance or prolong the antibody response, however, t cell responses were stronger. a ... | 1993 | 8411102 |
| mesangial proliferative glomerulonephritis induced in rats by a lentil lectin and its antibodies. | experimental glomerulonephritis was induced in rats to investigate the consequence of the antigen-antibody interaction on the surface of glomerular endothelial cells (gens). a lectin, lens culinaris hemoagglutinin (lch), was first planted in the left kidney by isolated perfusion of a left kidney, and then the circulation was reestablished. rabbit anti-lch antibodies were injected from the tail vein 3 minutes after the recirculation of the left kidney, and acute glomerulonephritis ensued. fifteen ... | 1993 | 8426083 |
| growth and survival of shigella flexneri in common bangladeshi foods under various conditions of time and temperature. | survival and growth of shigella flexneri were assessed in various foods, including boiled rice, lentil soup, milk, cooked beef, cooked fish, mashed potato, mashed brinjal, and raw cucumber. growth at 25 and 37 degrees c and survival at 5 degrees c were observed by viable counts on macconkey agar. the organism grew well in all tested foods and growth increased from 10(5) to 10(8) to 10(10) cells per ml or g within 6 to 18 h after inoculation at 25 and 37 degrees c. | 1993 | 8434933 |
| thermodynamics of monosaccharide binding to concanavalin a, pea (pisum sativum) lectin, and lentil (lens culinaris) lectin. | titration calorimetry measurements of the binding of methyl alpha-d-mannopyranoside (me alpha man), d-mannopyranoside (man), methyl alpha-d-glucopyranoside (me alpha glu), and d-glucopyranoside (glu) to concanavalin a (con a), pea lectin, and lentil lectin were performed at 281 and 292 k in 0.01 m dimethylglutaric acid-naoh buffer (ph 6.9) containing 0.15 m nacl and mn+2 and ca+2 ions. the site binding enthalpies, delta h, are the same at both temperatures and range from -28.4 +/- 0.9 (me alpha ... | 1993 | 8463297 |
| purification and partial characterization of 200 kda oncofetal antigen from radiation induced murine lymphocytic lymphoma. | 1. a 200 kda glycoprotein (gp200) oncofetal antigen was purified from solubilized membranes of a radiation-induced murine lymphocytic lymphoma cell line (xr11-5t), grown in syngeneic rfm mice, by successive gel chromatography of the active fraction on lentil lectin agarose, q- and s-sepharose and superose-12 using an fplc system. 2. a murine monoclonal antibody 115, produced by the syngeneic immunization of adult male c57bl/6n mice with 12-day mouse fetal cells, was used in a slot blot antibody ... | 1993 | 8467950 |
| dynamic light scattering studies of alpha iib beta 3 solution conformation. | the prototypical integrin receptor, alpha iib beta 3, isolated from the membrane fraction of human blood platelets by solubilization in triton x-100 (reduced) and affinity chromatography on lentil lectin-agarose, has been further purified by gel filtration chromatography in octyl glucoside to obtain the intact receptor complex in a form suitable for hydrodynamic measurements. the molecular weight [(6.0 +/- 0.2) x 10(3)] and stokes radius (2.3 +/- 0.1 nm) of detergent micelles formed in 0.03 m oc ... | 1993 | 8471605 |
| comparative effects of four legume species on plasma lipids and faecal steroid excretion in hypercholesterolaemic pigs. | the effect of four species of legume seeds on plasma cholesterol levels and faecal steroid excretion was studied in pigs. thirty-six growing boars were randomly allocated in groups of six to six diets which they ate continuously for 42 d. the diets fed were: 1, a semi-purified (sp; control group 1) diet; 2, sp + 10 g cholesterol/kg (control group 2); 3, 4, 5, 6, sp + cooked legumes (70:30, w/w; respectively baked beans (phaseolus vulgaris), peas (pisum sativum), lentils (lens culinaris medik.), ... | 1993 | 8489997 |
| isolation and characterization of the soluble and membrane-bound porcine cd44 molecules. | porcine cd44 was identified by the cross-reactive anti-human cd44 monoclonal antibody (mab) hermes-1. material which inhibited the hermes-1 mab binding to porcine lymphocytes was found in porcine intestinal efferent lymph and demonstrated to be a soluble form of porcine cd44. a protocol using non-affinity techniques was established to isolate this material from porcine lymph. the isolated soluble cd44 molecule consists of a single peptide with an apparent mw of about 50,000, containing the herme ... | 1993 | 8495972 |
| characterization of an eosinophilic leukemia cell differentiation factor (eldf) produced by a human t cell leukemia cell line, hil-3. | an adult t cell leukemia cell line, hil-3, constitutively secretes a factor which induces the phenotypical and functional eosinophilic differentiation of a human eosinophilic leukemia cell line, eol-1. biochemical characteristics of the factor, termed eosinophilic leukemia cell differentiation factor (eldf), were examined. eldf was precipitated by 35 to 65% saturated ammonium sulfate from the culture supernatants of hil-3 cells (hil-3 sup). eldf was eluted in a peak corresponding to a molecular ... | 1993 | 8500576 |
| immunoblot analysis of aspergillus fumigatus antigen with human antibodies and lectin probes. | components of culture filtrate antigen of aspergillus (a.) fumigatus were electrophoretically separated, transferred to hydrophobic durapore membrane, and then probed with patients' sera and lectins. using human sera from patients with aspergilloma and allergic bronchopulmonary aspergillosis (abpa), more than 13 igg binding components were identified. igg binding to high molecular weight components (hmw) of over 30 kda was observed in all patient sera tested, but igg binding to low molecular wei ... | 1993 | 8507937 |
| comparative analysis of the immunoprotective abilities of glycosylated and deglycosylated parainfluenza virus type 3 surface glycoproteins. | the role of carbohydrate moieties on the immunoprotective ability of parainfluenza virus type 3 (piv-3) haemagglutinin-neuraminidase (hn) and fusion (f) glycoproteins was tested in hamsters. hn and f proteins were purified from detergent-solubilized virus by lentil-lectin affinity chromatography and deglycosylated by treatment with endoglycosidase f (endo f). immunization of hamsters with either 1 or 5 micrograms of mock-treated (glycosylated) affinity-purified proteins elicited strong haemagglu ... | 1993 | 8277287 |