Publications

kinetics of antibody and immunoglobulin-producing cells appearing in popliteal lymph nodes of mice stimulated with horseradish peroxidase.		1977	561126
horseradish peroxidase transport from primate dental pulps.		1977	75216
an electron microscopic investigation of the surface coat of the electrocyte of electrophorus electricus.	the surface coat of the electrocyte of the main electric organ of electrophorus electricus was studied using cytochemical methods (periodic acid-silver methanamine, periodic acid-chromic acid-silver methenamine, periodic acid-thiosemicarbazide-silver proteinate, concanavalin a - horseradish peroxidase, ruthenium red, alcian-blue lanthanum nitrate, colloidal iron hydroxide and cationized ferritin). the surface of the electrocyte presents perpendicularly oriented tubular invaginations of the cell ...	1977	72610
cytochemical localization of acetylcholine receptors at the neuromuscular junction by means of horseradish peroxidase-labeled alpha-bungarotoxin.	the cytochemical localization of acetylcholine (ach) receptors at the neuromuscular junction was investigated with a procedure utilizing alpha-bungarotoxin (alpha-btx) labeled directly with horseradish peroxidase (hrp). following incubation of tissues in the conjugate and reaction for peroxidase, activity was observed on the junctional folds of the motor endplate. a uniform layer of reaction product approximately 15 nm thick occurred over the apical portions of the junctional folds. membranes at ...	1977	912399
vestibular projections to the nucleus intercalatus of staderini mapped by retrograde transport of horseradish peroxidase.	medullary afferent projections to the nucleus intercalatus of staderini have been studied by retrograde transport of horseradish peroxidase (hrp) from highly localized injections. this nucleus receives afferent projections particularly from the medial and descending vestibular nuclei as well as from the nucleus praepositus hypoglossi of both sides. the nucleus intercalatus of staderini represents therefore an area of integration for the vestibular systems of both sides.	1977	19605012
electron microscopic labeling of intercellular spaces in isolated tissue specimens with horseradish peroxidase.		1977	929637
magnetic circular dichroism studies on acid and alkaline forms of horseradish peroxidase.	the heme vicinities of the acid and alkaline forms of native (fd(iii)) horseradish peroxidase were investigated in terms of the magnetic circular dichroism (mcd) spectroscopy. the mcd spectrum of the acid form of native horseradish peroxidase was characteristic of a ferric high spin heme group. the resemblance in the mcd spectrum between the acid form and acetato-iron (iii)protoporphyrin ix dimethyl ester suggests that the heme iron of the acid form has the electronic structure similar to that i ...	1977	19085
a perfusion method of incubation to demonstrate horseradish peroxidase in bone.	a perfusion method of incubation to show horseradish peroxidase in the bone of young mice is presented. after perfusion fixation, the incubation medium is perfused from the descending aorta into the entire lower half of the animal. from the vessels there is good penetration of the medium into all tissues. this allows the preparation of any one perfused bone to ground-, semithin-, and ultrathin sections. differences in peroxidase distribution in the entire bone suggest regional differences in vas ...	1977	914657
the connections of the trigeminal and facial motor nuclei in the brain of the carp (cyprinus carpio l.) as revealed by anterograde and retrograde transport of horseradish peroxidase.	the connections of the rostral and caudal parts of the trigeminal and facial motor nuclei in the carp were studied with the horseradish peroxidase technique. following ionophoretic peroxidase injections in these motor nuclei, retrogradely labeled cells were observed together with anterogradely labeled motor cell processes. several cellular areas in thalamus, cerebellum and medulla oblongata were shown to project to the v and vii motor nuclei. labeled cells were found in the inferior lobe and the ...	1977	903419
anatomical and physiological characterization of avian sympathetic cardiac efferents.	in the preceding paper6 describing the right sympathetic cardiac nerve of the pigeon we reported a fast compound action potential component with no chronotropic effect, and single unit recordings from sympathetic postganglionic neurons demonstrated that this action potential component was not reflected in the antidromic postganglionic latencies. electron microscopy then indicated the presence of numerous myelinated fibers in the nerve, and together these findings suggested the existence of a sub ...	1977	884549
nuclear magnetic resonance studies of hemoproteins. interaction of horseradish peroxidase with indolepropionic acid.		1977	891962
foveal sparing. new anatomical evidence for bilateral representation of the central retina.	the pattern of retinal ganglion cell projections to the dorsal lateral geniculate nuclei has been demonstrated with horseradish peroxidase neuronography. a 1 degree-wide strip centered on the vertical meridian has been found in which ipsilaterally and contralaterally projecting ganglion cells intermingle. this strip expands to a width of 3 degrees at the fovea, since mixing of horseradish peroxidase-labeled and unlabeled ganglion cells was found in a band approximately 0.5 degrees wide along bot ...	1977	407893
[kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase].	a kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase within the ph range of 3.7-9.0 has been carried out. it was shown that the reaction of o-dianisidine peroxidase oxidation obeys the michaelis--menten kinetics; the kcat and km values within the ph range used were determined. the optimum of peroxidase catalytic activity during o-dianisidine oxidation was observed at ph 5.0-6.0. the kinetic pattern of the reaction is discussed. it was demonstr ...	1977	20989
[the reaction of holo-, apo- and coenzyme of the peroxidase to heating (author's transl)].	the thermal reaction of the two components of the horseradish peroxidase--the apoenzyme and the prosthetic group--with that of the holoenzyme were compared. from this we conclude that the thermal inactivation of the peroxidase in aqueous solution is not caused by a change of the apoprotein alone, but rather by participation of the entire three-dimensional structure including the prosthetic group. spectrophotometric studies of the inactivation process in the holoenzyme revealed that the absorbanc ...	1977	910551
ultrastructural localization of a nerve growth factor-horseradish peroxidase (ngf-hrp) coupling product after retrograde axonal transport in adrenergic neurons.		1977	69476
perihypoglossal and secondary vestibular projections to lobules vi and vii of the cerebellar cortex: an hrp study.	horseradish peroxidase (hrp) was injected into (1) lobule vi; (2) lobule vii; (3) lobule vi and vii of the cat's cerebellar vermis. marked cells were found in two groups of nuclei related to the vestibulo-ocular system; (a) the three perihypoglossal nuclei: praepositus hypoglossi, nucleus of roller and intercalatus of staderini and (b) two areas of the secondary vestibular complex located within the medial and the descending vestibular nuclei. it is concluded that fibers from the perihypoglossal ...	1977	19604979
an enzyme-linked immunoassay of testosterone.	an enzyme-linked immunoassay of testosterone is described. the conjugation of testosterone with high specific activity horseradish per-oxidase and a highly sensitive assay for this enzyme having previously been studied, here we describe the immobilization of the anti-testosterone antibody and the development of assay conditions permitting the determination of 50 pg to 1.5 ng testosterone in one working day. in this work attention has been paid to keeping the assay method as simple as possible. t ...	1977	919013
characterization of the pigeon isthmo-tectal pathway by selective uptake and retrograde movement of radioactive compounds and by golgi-like horseradish peroxidase labeling.		1977	69469
effect of mixed solvents on the formation of horseradish peroxidase compound i. the importance of diffusion-controlled reactions.	in aqueous glycerol solutions, the rate constant for the formation of compound i of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. however, whem m-chloroperbenzoic acid is used to form compound i, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. the problems in obtaining accurate information about diffusion-controlled reactions are discussed as is the possible significance of diffusion control. ethanol has been used in previo ...	1977	880290
slow rate transport of horseradish peroxidase in toads.		1977	69468
automated fluorometric analysis of galactose in blood.	in galactosemia, prevention of mental retardation depends on early recognition of the disorder and institution of dietary restriction of galactose. we describe an automated fluorometric micromethod for galactose in whole blood spotted on filter paper. galactose is oxidized by galactose oxidase to d-galacto-hexadialdose and h2o2 and measured as the highly fluorescent condensation product of homovanillic acid formed when h2o2 is acted upon by horseradish peroxidase. the procedure is 10-fold more s ...	1977	870260
[thermal inactivation and storage behavior of technologically important enzymes. iii. effect of reagents added to peroxidase and lipoxygenase].	the influence of milieu factors on the thermal inactivation of peroxidase and lipoxigenase was investigated. cationogenic, anionogenic, non-ionogenic and amphoteric tensides were more or less effective in inactivating horseradish peroxidase. most effective in this respect were lecithine and monoglyceride, both capable of swelling in water. in presence of lecithine, peroxidase was inactivated already at 0 degrees c and ph 4.0. linoleic acid was more efficient in an oxygen stream than in presence ...	1977	408986
histochemical and ultrastructural studies in fibrodysplasia ossificans progressiva (myositis ossificans progressiva).	by light microscopy the subdermal nodule of a patient with fibrodysplasia ossificans progressiva (fop) had a fibromatoid histologic appearance. the cytoplasm of the cells stained strongly for mannose-rich glycoprotein with the concanavalin a-horseradish peroxidase (con a-hrp) method. the tumors also exhibited abundant hyaluronidase-digestible mucopolysaccharide in the interstitium with various basic staining reagents. this material appeared to consist principally of hyaluronic acid or chondroiti ...	1977	141214
[thermal inactivation and storage behavior of technologically important enzymes. i. horseradish and spinach peroxidase].	the thermal inactivation and storage behaviour for horseradish and spinach peroxidases were investigated in defined systems, in spinach also within its natural environment. the inactivation curves of either enzyme show a sharp bend which is clearly visible at low, but not at higher temperatures. the d-values were taken from the inactivation curves. z-values resulting from the d-values were 25.5 degrees c for horseradish peroxidase, 13 degrees c for isolated peroxidase and 18 degrees c for peroxi ...	1977	19887
evaluation of the horseradish peroxidase-scopoletin method for the measurement of hydrogen peroxide formation in biological systems.		1977	883627
the organization of the pulvinar in the grey squirrel (sciurus carolinensis). i. cytoarchitecture and connections.	the posterior neocortex in the grey squirrel, sciurus carolinensis, includes an extensive region which receives projections from the pulvinar. previous studies have demonstrated that this cortical region can be subdivided on the basis of differences in cytoarchitecture and electrophysiologically defined representations of the visual field. the main purpose of the present paper was to determine whether these cortical subdivisions could be related to corresponding subdivisions in the pulvinar. the ...	1977	856889
a new and sensitive staining method for axonally transported horseradish peroxidase (hrp) in the pigeon visual system.		1977	67876
active site models of horseradish peroxidase compound i and a cytochrome p-450 analogue: electronic structure and electric field gradients.		1977	853195
mechanism of thyroxine-mediated oxidation of reduced nicotinamide adenine dinucleotide in peroxidase-h2o2 system.	the oxidation of reduced nicotinamide adenine dinucleotide (nadh) by the horseradish peroxidase (hrp)-h2o2 system is greatly increased by the addition of thyroxine or related compounds. on the basis of a study of the rate of nadh oxidation in the presence of various concentrations of thyroxine, it is clear that thyroxine acts as a catalyst for nadh oxidation. spectral changes of a hrp-h2o2 complex (compound i) indicate that thyroxine acts as an electron donor to both compounds i and ii. the rate ...	1977	192281
analysis and computer simulation of aerobic oxidation of reduced nicotinamide adenine dinucleotide catalyzed by horseradish peroxidase.	under suitable experimental conditions the aerobic oxidation of nadh catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. a trace amount of h2o2 present in the nadh solution brought about initial burst in the formation of oxyperoxidase. about 2 mol of oxyperoxidase was formed per mol of h2o2. when a considerable amount of the ferric enzyme still remained, the initial burst was followed by an induction phase. in ...	1977	192280
[catalytic properties and thermostability of horseradish peroxidase covalently bound with sepharose by carbohydrate residues of the enzyme].	a procedure for isolation of stabilized preparations of peroxidase, covalently bound with ah-sepharose 4b by the enzyme carbohydrate residues, which had been previously oxidized by iodine acid, is proposed. the catalytic properties and thermostability of soluble oxidized and immobilized peroxidase has been studied and a comparison of these properties with those of the native enzyme has been made. it has been demonstrated that the oxidation of the carbohydrate residues dose not affect eh km value ...	1977	889967
[development of arrangement and degradation of the system of rough endoplasmic reticulum in antibody-producing plasma cells].	during the immune response of the cells of murine omentum to horseradish peroxidase which had been administered intraperitoneally together together with endotoxin or endotoxoid acting as an adjuvans the plasma cells showed several different patterns of the rough endoplasmic reticulum [rer] system. irregular to bizarre rer structures occurred plasma cells during the initial phase of the immune reaction only, whereas the plasma cells found at later stages had characteristic highly organized rer sy ...	1977	597922
retrograde axonal transport of horseradish peroxidase from cornea to trigeminal ganglion.	horseradish peroxidase (hrp) was dripped on the scarified left cornea of adult mice. twenty-four hours later the animals were fixed by vascular perfusion and frozen sections cut from both trigeminal ganglia. after incubation for peroxidase activity labelled nerve cells were restricted to the medial ophthalmic part of the ganglion ipsilateral to hrp administration. if the scarification was omitted no neuronal labelling was observed. this labelling of the neurons is most probably the result from a ...	1977	300978
plant glycoprotein biosynthesis. uridine diphosphate n-acetyl-glucosaminyltransferase from horseradish root.	a particulate enzyme preparation from horseradish root tissue was shown to catalyze the transfer of 2-acetamido-2-deoxy-d-[14c1]glucose from uridine diphosphate 2-acetamido-2-deoxy-d-[14c1]glucose to an exogenous acceptor molecule derived from horseradish peroxidase. the acceptor was produced from purified peroxidase by the action of a mixture of glycoside hydrolases covalently bound to sepharose. the membrane preparation containing the transferase was purified approximately 12-fold by aqueous t ...	1977	403958
polymerisation of oestradiol by potassium ferricy anide and by horseradish peroxidase.		1977	862904
vesicular transport of horseradish peroxidase from brain to blood in segments of the cerebral microvasculature in adult mice.	the transfer of protein from the cerebral ventricles to the parenchymal bloodstream in mice was studied by electron microscopy. after perfusion with the protein tracer horseradish peroxidase (hrp; m.w. approx. 40,000) through the cerebral ventricles, the tracer penetrated the ependymal lining of the ventricles and was found in the extracellular space of the neuropil close to the ependyma. hrp was also seen in the vascular basement membrane and in endothelial vesicles opening at the abluminal end ...	1977	843935
amino-acid sequences of heme-linked, histidine-containing peptides of five peroxidases from horseradish and turnip.	in a previous paper we have characterized five plant peroxidases, p1, p2, p3 and p7 of turnip and horseradish isoperoxidase c by peptide mapping studies, and only found two highly homologous sequences present in all. both contained histidine. the findings supported previous suggestions of two histidine sequences nearthe peroxidase heme prostetic group. in the present paper we present the amino acid sequences around the histidine residues of all four turnip peroxidases, i. e. of 25 residues aroun ...	1977	849740
the organization of the facial nucleus of the brush-tailed possum (trichosurus vulpecula).	the facial nucleus of the brush-tailed possum has been studied using nissl staining and the horseradish peroxidase (hrp) retrograde tracing technique. in nissl stained sections the nucleus is seen to comprise five distinct subnuclei. injections of hrp into individual facial muscle groups have shown that these subnuclei reflect the peripheral innervation pattern of efferents from this nucleus. although in most cases, injection of hrp into a single facial muscle group resulted in the labelling of ...	1977	65363
efferent projections of the main and the accessory olfactory bulb in the tree shrew (tupaia glis).	the projections of the main and the accessory olfactory bulb in the tree shrew (tupaia glis) have been analyzed with anterograde degeneration and autoradiographic methods for identifying axonal projections, and with the horseradish peroxidase method for identifying the distribution of neurons from which these projections originate. the cytoarchitectonic features of the paleocortical areas which receive projections from the main and the accessory olfactory bulb have also been described. the effer ...	1977	65361
free radical intermediate in the n-demethylation of aminopyrine by horseradish peroxidase-hydrogen peroxide.		1977	190040
sensitized photooxidation of low spin horseradish peroxidase.		1977	191009
electron microscopic observations of cell coat of childhood leukemic cells using concanavalin a-horseradish peroxidase method.		1977	327742
horseradish peroxidase. xxv. an electron spin resonance study of the low temperature photochemical reaction of compound i.		1977	189760
protein-accumulating cells and dilated cisternae of the endoplasmic reticulum in three glucosinolate-containing genera: armoracia, capparis, drypetes.	three glucosinolate-containing species, armoracia rusticana gaertner, meyer et scherbius (brassicaceae), capparis cynophallophora l. (capparaceae) and drypetes roxburghii (wall.) hurusawa (euphorbiaceae), are shown by both light and electron microscopy to contain protein-accumulating cells (pac). the pac of armoracia and copparis (former "myrosin cells") occur as idioblasts. the pac of drypetes are usual members among axial phloem parenchyma cells rather than idioblasts. in drypetes the vacuoles ...	1977	24420656
involvement of malate, monophenols, and the superoxide radical in hydrogen peroxide formation by isolated cell walls from horseradish (armoracia lapathifolia gilib.).	peroxidase associated with isolated horseradish cell walls catalyzes the formation of h2o2 in the presence of nadh. the reaction is stimulated by various monophenols, especially of coniferyl alcohol. nadh can be provided by a bound malate dehydrogenase. this system is capable of polymerizing coniferyl alcohol yielding an insoluble dehydrogenation polymer. nadh was found to be oxidized by two different mechanisms, one involving mn(2+), monophenol, and the superoxide radical o2 (·-) in a reaction ...	1977	24420401
photochemical dimerization of ferulic acid by chloroplasts from sorghum.	sorghum (sorghum bicolor) chloroplasts, lamellar fragments, and triton x-100 solubilized preparations catalyze a blue and red light-sensitized oxidation of ferulic acid to its beta-beta-linked dimer and its hydrolysis product, the acid dimer. exogenous superoxide dismutase had no effect, and catalase and 1 to 10 mm kcn inhibited this photooxidative dimerization only in detergent-treated chloroplasts. it is postulated that the final oxidant is h(2)o(2), formed by light-induced photosystem i elect ...	1977	16659796
selective enzymatic hydrolysis of nerve terminal phospholipids by beta-bungarotoxin: biochemical and morphological studies.	beta-bungarotoxin, a presynaptic protein neurotoxin isolated from the venom of the snake bungarus multicinctus, modifies release of neurotransmitter at the neuromuscular junction. the toxin has a potent phospholipase activity toward both natural membranes and phospholipid liposomes. studies of ionic requirements and selective chemical modification demonstrate that beta-bungarotoxin can only modify synaptic transmission under conditions in which the phospholipase is active. morphological studies ...	1977	928454
technical considerations on the use of horseradish peroxidase as a neuronal marker.		1977	917271
retrograde transport of horseradish peroxidase from the nucleus posterior medialis hypothalami to the hippocampus and the medial septum in the pigeon.		1977	896046
hydroxylation of tyrosine by plant peroxidase and mushroom tyrosinase, with and without hydrazine, to retard the oxidation of dopa.	tyrosine was oxidized to dopa by horseradish peroxidase and mushroom tyrosinase. the first step of hydroxylation of tyrosine in the synthesis of melanin was demonstrated by isolation of dopa from the reaction mixture using hydrazine as a selective retardant.	1977	410043
the chemical properties of out-of-equilibrium states of proteins and the role of these states in protein functioning.	the out-of-equilibrium states of several iron-containing proteins (cytochromes c of different origin, haemoglobin, myoglobin, ferredoxin and other non-haem iron proteins, cytochrome c oxidase, horseradish peroxidase) were recorded after fast changes in the active centre (electron reduction of iron, ligand dissociation). strained states result in which the active centre has already been changed and undergone vibrational relaxation but the main part of protein globule is in the 'old', now out-of-e ...	1977	215393
a bilateral cortical projection to the superior colliculus in the cat.	projections from the cerebral cortex to the superior colliculus (sc) of the cat have been determined by injecting horseradish peroxidase (hrp) unilaterally into sc. in addition to labelled cells found in the visual areas ipsilateral to the injection site, hrp-positive neurones were observed in the lateral bank of the suprasylvian sulcus of both hemispheres. this region, the suprasylvian fringe, which is part of the auditory cortex, apparently projects bilaterally to the sc.	1977	19604912
analysis of collateral projections with a double retrograde labeling technique.	a method is described whereby collateral innervation by a single cell type may be determined by double retrograde labeling with horseradish peroxidase (hrp) and tritiated bovine serum albumin ([(3)h]bsa). the projections from the entorhinal area to the hippocampal formation were analyzed as a model system, since layer iii pyramidal cells project bilaterally to the hippocampus. following hrp injections into the hippocampus of one side, and [(3)h]bsa injections into the opposite hippocampus, doubl ...	1977	19604962
differential sensitivity between blue and brown reaction procedures for hrp neurohistochemistry.	four rhesus monkeys and two rats were injected with horseradish peroxidase (hrp). adjacent sections were stained with a 3,3'-diaminobenzidine tetrahydrochloride (dab) and a benzidine dihydrochloride (bdhc) procedure which yields a blue reaction product at sites of hrp activity. the number of labeled neurons was counted within the same regions of the adjacent sections with bright-field and dark-field illumination. sections processed with the bdhc procedure consistently contained more labeled neur ...	1977	19604963
staining of the dorsal root projection to the cat's dorsal horn by anterograde movement of horseradish peroxidase.	a modification of the horseradish peroxidase method was used to stain selectively dorsal root fibers entering the spinal cord of the cat. this technique stains intact terminal arborizations. using this method to examine the primary afferent supply to lamina ii of the lumbosacral segments, we observed that both thick primary afferent collaterals (2-3 mum), entering by way of the dorsal column, and thinner primary afferent collaterals (<1 mum), entering by way of lissauer's tract, form terminals i ...	1977	19604978
differential localization of rabbit's flocculus purkinje cells projecting to the medial and superior vestibular nuclei, investigated by means of the horseradish peroxidase retrograde axonal transport.	the retrograde axonal transport of horseradish peroxidase (hrp) was used to study distribution of those purkinje cells in the flocculus which project to the medial and superior vestibular nuclei of albino rabbits. hrp was injected electrophoretically into those nuclei and after survival for 24-48 h the flocculus was examined microscopically. it was found that purkinje cells projecting to the medial nucleus were located relatively rostrally, while those projecting to the superior nucleus lay rela ...	1977	19605007
kinetics of the oxidation of p-aminobenzoic acid catalyzed by horseradish peroxidase compounds i and ii.	the kinetics of p-aminobenzoic acid oxidation catalyzed by horseradish peroxidase compounds i and ii was investigated intensively as a function of ph at 25 degrees in aqueous solutions of ionic strength 0.11. all of the rate data were collected from single turnover experiments involving reactions of a single enzyme compound. in reactions of both compounds, deviations from first order behavior with respect to the enzyme were observed at high ph values which were explained in terms of a free radic ...	1977	235530
role of phenolic inhibitors in peroxidase-mediated degradation of indole-3-acetic acid.	7-hydroxy-2,3-dihydrobenzofuran derivatives, metabolites of a carbamate insecticide carbofuran, and five other phenolic inhibitors of indoleacetic acid (iaa) oxidase interfered with iaa-induced spectral change in the soret band of horseradish peroxidase (hrp). the onset of iaa degradation required transformed hrp intermediates. the inhibitors, when added before iaa, protected hrp from reacting with iaa, thus preventing formation of highly reactive enzyme intermediates, and consequently, iaa degr ...	1977	16659854
intraaxonal transport of horseradish peroxidase in the sympathetic nervous system.	following a single injection of horseradish peroxidase (hrp) into the superior cervical ganglion (scg) of the rabbit, the uptake and anterograde transport of this label was confirmed in the ganglion cell bodies, postganglionic axons, and preterminal and terminal ending axons in the ciliary processes of the eye. from the same injection site the intraaxonal hrp reaction product was demonstrated in myelinated axons, presumably by retrograde transport. intracytoplasmic hrp was identified in large, s ...	1977	64283
identification of cells or origin of non-primary afferents to the dorsal column nuclei of the cat.	in order to identify cells of origin in the spinal cord of non-primary afferents to the dorsal column nuclei (dcn), the retrograde transport of horseradish peroxidase (hrp) has been utilized in adult cats. 10 to 30% hrp was injected bilaterally (0.6 mul per side) in the dorsal medulla of nine cats. in most instances the spread of the injected enzyme extended a few millimeters rostrocaudally and infiltrated the dcn as well as other nuclei and fiber tracts. labelled cells in these cases are numero ...	1977	64365
anatomical studies of a temporal visual area in the rabbit.	using fink-heimer, autoradiographic and horseradish peroxidase techniques, the connections of a temporal visual cortical region of the rabbit were explored. the temporal visual area covers portions of areas t1 and t2, and is reciprocally connected with the posterior nucleus and suprageniculate nuclei of the thalamus. it was also shown that the temporal visual area projects to a similar region in the opposite hemisphere, and to intermediate laminae of the superior colliculus. the temporal visual ...	1977	64476
functional organization of the vestibular input to the anterior and posterior cerebellar vermis of cat.	1. responses evoked by electrical stimulation (auditory division of the viiith nerve sectioned chronically) and natural stimulation of the vestibular apparatus were recorded in the anterior and posterior cerebellar vermis of cats anesthetized with ketamine or nembutal. under ketamine the functional state of the cerebellar cortex was similar to that of the decerebrate or encéphale isolé preparation. 2. vestibular-evoked responses were found bilaterally throughout the vermis (lob. i-x) and parts o ...	1977	65291
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iv. the projection to the anterior lobe.	following injections of horseradish peroxidase (hrp) in the cerebellar cortex of the anterior lobe of the cat, the distribution of labeled cells in the inferior olive was mapped. the findings largely confirm those made previously in studies of olivary retrograde cell loss following cerebellar ablations (brodal, '40b). in addition, they reveal further olivary areas projecting onto the anterior lobe, and permit a more detailed analysis of the pattern in this projection. concerning major points the ...	1977	65365
brain stem afferents to the fastigial nucleus in the cat demonstrated by transport of horseradish peroxidase.	although retrograde and anterograde degeneration studies have provided important information concerning brain stem afferents to the fastigal nucleus (fn), these data may be incomplete and should be confirmed by axonal transport methods. attempts were made to inject horseradish peroxidase (hrp) unilaterally into the fn in a series of adult cats. animals were perfused with dextran and a fixative solution of paraformaldehyde and glutaraldehyde in 0.1 m phospate buffer. representative sections were ...	1977	65366
the vestibular complex of the american opossum didelphis virginiana. ii. afferent and efferent connections.	we have demonstrated the connectivity of the opossum's vestibular nuclei using degeneration, autoradiographic and horseradish peroxidase techniques and have found it to be generally comparable to that reported for the cat. apart from the primary input described in part i of our study, the cerebellum provides the major source of afferent connections to the vestibular complex. axons from the cerebellar cortex distribute mainly to vestibular areas which receive no primary afferent projections, e.g. ...	1977	65367
ultrastructural visualization of cellular carbohydrate components by means of lectins on ultrathin glycol methacrylate sections.	a method for the visualization of cellular carbohydrate components by both light and electron microscopy using lectins on glycol methacrylate sections is proposed. this method, which is an application of the lectin-peroxidase affinity technique, solves the problem of limited penetration when it is attempted to demonstrated lectins receptors within the tissue block. following partial dissolution of glycol methacrylate from thin sections using alcohol, they are incubated successively with lectin ( ...	1977	65417
organization of the facial nerve nucleus in the cat.	the location of efferent neurons in the brain stem of newborn kittens supplying the facial muscles was studied by means of horseradish peroxidase (hrp) used as a tracer of retrograde protein transport. three to 15 microliters containing 0.3 to 1.5 mg of hrp was injected into the orbicularis oris and orbicularis oculi muscles of six kittens and in the seventh kitten, the ventral ramus of the facial nerve was transected and hrp was instilled on the cut end of the nerve. after a survival time of 24 ...	1977	65707
brain stem projections to lobule vii of the posterior vermis in the squirrel monkey: as demonstrated by the retrograde axonal transport of tritiated horseradish peroxidase.		1977	66085
localization of abductor and adductor fibers in the kitten recurrent laryngeal nerve: use of a variation of the horseradish peroxidase tracer technique.		1977	66152
retrograde axonal transport of horseradish perioxidase in afferent fibers of the inferior mesenteric ganglion of the guinea pig. identification of the cells of origin in dorsal root ganglia.		1977	66970
orthograde and retrograde axoplasmic transport during acute ocular hypertension in the monkey.	orthograde and retrograde axoplasmic transport have been studied in the optic nerve heads of 37 macaca fascicularis eyes with normal or elevated intraocular pressure (iop) produced by cannulation of the anterior chamber. orthograde transport was labeled by 3h-amino acids injected intravitreally and incorporated into retinal ganglion cell proteins. retrograde transport was studied in the same eyes by injecting horseradish peroxidase (hrp) into one or both optic tracts and dorsal lateral geniculat ...	1977	67096
identification of different size motoneurons labeled by the retrograde axonal transport of horseradish peroxidase.	three main groups of motoneurons of different size have been labeled in adult cats by using the method for retrograde axonal transport following injection of horseradish peroxidase in the medial gastrocnemius and soleus muscles. in particular small, medium-size and large neurons which probably correspond respectively to gamma, small alpha and large alpha motoneurons innervating the calf muscles, have been identified and the corresponding area measured.	1977	67590
the organization of thalamocortical relay neurons in the rat ventrobasal complex studied by the retrograde transport of horseradish peroxidase.		1977	68039
commercial horseradish peroxidase degrades myelin encephalitogenic protein during coupling for immunohistochemical studies.	conjugates of myelin encephalitogenic basic protein (ep) and commercial horseradish peroxidase (hrp) have been used for immunohistochemical demonstrations of anti-ep antibody in animals with experimental allergic encephalomyelitis. we performed gel electrophoresis studies on ep-hrp conjugates prepared with glutaraldehyde and on mixtures of ep and hrp incubated without glutaraldehyde. the results show that under conditions of one-and two-step coupling hrp causes rapid loss of the native ep band, ...	1977	68067
[study of the vagal innervation of the cat stomach by means of retrograde axoplasmic transport of horseradish peroxidase--on the localization of the origins of the preganglionic fibers].		1977	68782
retrograde axonal transport of horseradish peroxidase. uptake at mouse neuromuscular junctions following systemic injection.	eye muscles were examined histochemically for the presence of horseradish peroxidase (hrp) activity after intravenous injections of large doses of this tracer protein. hrp had penetrated through the blood vessels and diffused into the areas of motor end plates, where it outlined axon terminals at the synaptic clefts. hrp was incorporated into pinocytotic vesicles in the axons from where an intraaxonal transport in the retrograde direction to the nerve cell bodies in the brainstem followed. accum ...	1977	69382
demonstration of bilateral claustro-cortical connections in the cat with the method of retrograde axonal transport of horseradish peroxidase.	the claustrum of the cat was studied to determine the extent of its projection to the cerebral cortex. neurons in the bilateral dorsal claustrum are labeled by retrograde axonal transport of horseradish peroxidase (hrp) from injection sites of different neocortical areas with preponderance to the ipsilateral side. it is revealed that the dorsal claustrum projects to almost entire regions of the bilateral neocortex and that distribution of labeled neurons in the bilateral claustrum has topographi ...	1977	69423
identification of cells of origin of tectopontine fibers in the cat superior colliculus: an experimental study with the horseradish peroxidase method.	the pontine projections from the superior colliculus in the cat have been studied by means of retrograde axonal transport of horseradish peroxidase (hrp). following injections of hrp in the dorsolateral pontine nucleus, where the tectopontine fibers terminate, a fair number of labeled cells are found throughout the rostrocaudal extent of the ipsilateral superior colliculus. relatively few of the labeled cells are of medium size (25-40 micron in diameter), more than 80% are small (10-25 micron), ...	1977	69477
the effects of monocular deprivation on different neuronal classes in the lateral geniculate nucleus of the cat.	retrograde axonal transport of horseradish peroxidase (hrp) was used to identify two populations of cells in the lateral geniculate nucleus (lgn) of the cat. hrp was injected into area 17 and 18 separately in the same animals, and the neuronal somata giving rise to thalamo-cortical axons, identified by the presence of granular hrp reaction product within them, were measured. the mean size of lgn neurones labelled by injections in area 17 ("17-relay" cells) was less than of neurones filled from a ...	1977	69546
ultrastructural immunoenzymatic study of alpha-fetoprotein-producing cells in the human fetal liver.	cells producing alpha-fetoprotein in human fetal liver have been studied with specific horseradish peroxidase labeled immunoglobulins. under light microscopy, the alpha-fetoprotein is strictly localized in the cytoplasm of certain hepatocytes, distributed randomly in the hepatic lobule. ultrastructural examination of thesame cells shows that the alpha-fetoprotein is present within the cytoplasm. ultrastructural differences are described in hepatocytes according to whether or not the cell is prod ...	1977	69654
axoplasmic transport in ocular hypotony and papilledema in the monkey.	orthograde and retrograde axoplasmic transport were studied in optic nerve heads of seven hypotensive macaca fascicularis eyes. orthograde transport was studied by radioautography after intravitreal radioisotope injections. retrograde transport was studied in the same eyes by horseradish peroxidase injection into the dorsal lateral geniculate nuclei or optic tracts. three eyes had developed marked papilledema before injections. orthograde axoplasmic transport was blocked in swollen axons of the ...	1977	70200
bidirectional movement of horseradish peroxidase and the demonstration of reciprocal thalamocortical connections.		1977	70257
evidence for direct synaptic interconnections between cat spinal alpha-motoneurons via the recurrent axon collaterals: a morphological study using intracellular injection of horseradish peroxidase.	by using intracellular injection of horseradish peroxidase into cat triceps surae alpha-motoneurons we have been able to trace the motor axons as well as the recurrent motor axon collaterals with their synaptic terminals both with the light and electron microscope. in addition to the expected projection to the 'renshaw cell area', the triceps surae motor axon collaterals were frequently found to terminate within the motor nuclei, where at least some of the terminals made direct synaptic contact ...	1977	70258
role of gangliosides in the uptake and retrograde axonal transport of cholera and tetanus toxin as compared to nerve growth factor and wheat germ agglutinin.	previous investigations have shown that tetanus toxin is transported retrogradely in all peripheral neurons whereas the transport of ngf is confined to adrenergic and sensory neurons. other macromolecules with molecular weights and general physiochemical properties similar to ngf and tetanus toxin (e.g., cytochrome c, insulin, horseradish peroxidase and bovine serum albumin) are not transported to a detectable extent if injected in comparable molar concentrations. for tetanus toxin, which is tra ...	1977	70259
retrograde axonal transport of horseradish peroxidase for determining motor projection patterns to the developing limb in xenopus.	horseradish peroxidase (hrp) injected into developing limb buds of xenopus laevis tadpoles is carried by retrograde axonal transport to the somata of motoneurones in the ventral horn. small injection of 10% hrp were found to remain well localised to specified sites in the limb bud. two types of labelled cells were found: diffusely labelled and granular labelled. diffusely labelled cells result from axonal damage in the presence of hrp. granular labelled cells result only from uptake of hrp from ...	1977	70260
[projections of bulbar reticular nuclei and raphe nuclei to lobules vi and vii of cat cerebellar cortex].	injections of horseradish peroxidase were made in lobules vi and vii of the cerebellar vermis (in the cat). serial frontal sections of the brain stem reveal that the lateral and paramedian nuclei were essentially projected onto lobule vi, and the projections from the raphe nuclei onto lobule vii.	1977	70281
origin of the direct hippocampus--anterior thalamic bundle in the rat: a combined horseradish peroxidase--golgi analysis.		1977	71242
localization of thyroiglobulin antigenicity in rat thyroid sections using antibodies labeled with peroxidase or 125i-radioiodine.	in the hope of localizing thyroglobulin within focullar cells of the thyroid gland, antibodies raised against rat thyroglobulin were labeled with the enzyme horseradish peroxidase or with (125)i-radioiodine. sections of rat thyroids fixed in glutaraldehyde and embedded in glycol methacrylate or araldite were placed in contact with the labeled antibodies. the sites of antibody binding were detected by diaminobenzidine staining in the case of peroxidase labeling, and radioautography in the case of ...	1977	71304
movement of horseradish peroxidase in the cornea, sclera and the anterior uvea.	the anterior chambers of rabbit, monkey and human eyes were perfused with horseradish peroxidase. the perfusion was started immediately after enucleation of the monkey and human eyes, while the rabbit eyes were perfused in situ after the animals had been killed. comparative results were obtained after 1 h or perfusion and were mainly based on frozen sections. intensive staining was found in the descemet's membrane, the sclera and the iridial stroma, considerably more than in the corneal stroma a ...	1977	71844
the permeability of the human ciliary and iridial epithelium to horseradish peroxidase. an in vitro study.	the permeability of the human ciliary epithelium to horseradish peroxidase (po) has been studied in vitro with the electron microscope. ciliary body and iris specimens were obtained from freshly enucleated eyes. po was applied at the stromal side of the epithelium, and was left for 120 min. the movement of po through the intercellular spaces of the human ciliary epithelium was blocked apically in the lateral intercellular spaces of the non-pigmented epithelial cells, indicating that these cells ...	1977	71845
arborization of initial axon collaterals of spinocervical tract cells stained intracellularly with horseradish peroxidase.	the initial axon collateral arborizations of two feline spinocervical tract cells have been analysed after intracellular staining with horseradish peroxidase. the images of the cells were reconstructed in the light microscope from transverse serial sections. the cell bodies were situated in laminae iv or iii of the lumbar dorsal horn. each cell exhibited one initial collateral. these emerged from the parent axons at distances of about 310 and 580 micron, respectively, from the cell body. the col ...	1977	71934
the pontine projection to the crebellar anterior lobe. an experimental study in the cat with retrograde transport of horseradish peroxidase.		1977	72000
detection of epstein-barr virus antigens with enzyme-conjugated antibody.	monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, im (mu), igg (gamma) and iga (alpha), were compared for their ability to detect human ig classes possessing specificity for epstein-barr virus (ebv) viral capsid antigens (vca) in a chronically infected human lymphoid cell line, p3j-hrik. it was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting mos ...	1977	72045
observations on labeling of neuronal cell bodies, axons, and terminals after injection of horseradish peroxidase into rat brain.		1977	72079
the olivocerebellar projection studied with the method of retrograde axonal transport of horseradish peroxidase. v. the projections to the flocculonodular lobe and the paraflocculus in the rabbit.	hrp was injected in the flocculonodular lobe and the paraflocculus in the rabbit to determine the areas of the inferior olive which project onto these cerebellar regions. following injections in the flocculus labeled cells occurred in the dorsal cap and the rostralmost tip of the medial accessory olive. following injections in the nodulus labeled cells were likewise found in the dorsal cap, but in addition in the rostralmost part of the dorsomedial cell column and the adjoining part of the media ...	1977	72080
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. vi. the projection onto longitudinal zones of the paramedian lobule.	microinjections (30-50 nl) of a horseradish peroxidase (hrp) suspension of 25% (wt./vol.) were made in different folia of the paramedian lobule of cats, and the sites of occurrence of labeled cells in the inferior olive were precisely determined. in each case only a small number of cells are labeled, aggregated in a minute area. the labeled cells are found within three only of the four olivary areas previously determined (brodal et al., '75) to project onto the paramedian lobule (fig. 1): one ar ...	1977	72081
salivatory neurons innervate the submandibular and sublingual glands in the rat: horseradish peroxidase study.		1977	72589
cerebeller afferents from neurons in motor nuclei of cranial nerves demonstrated by retrograde axonal transport of horseradish peroxidase.		1977	72590
somatopetal transport of horseradish peroxidase (hrp) in the peripheral and central branches of dorsal root ganglion cells.	the axonal transport of hrp in both the peripheral and central branches of dorsal root ganglion cells was studied in rats. for studying axonal transport in the peripheral branch hrp as a dry substance was applied to the peroneal nerve injured either by teasing, by cutting or crushing. after a short survival time (22 h) mainly small spinal ganglion cells of the corresponding segments were labelled, while after a prolonged survival time (70 h) mainly large cells were labelled. these labelling diff ...	1977	72611
afferents to the entorhinal cortex of the rat studied by the method of retrograde transport of horseradish peroxidase.		1977	72679