Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| mycoplasma infection and rheumatoid arthritis: analysis of their relationship using immunoblotting and an ultrasensitive polymerase chain reaction detection method. | to examine the relationship between infection with mycoplasma and the development of rheumatoid arthritis (ra) and juvenile rheumatoid arthritis (jra). | 1997 | 9214421 |
| mycoplasma in urine and blood following catheterisation of patients undergoing vascular surgery. | the purpose of this investigation was to determine if mycoplasmas enter the bloodstream after urinary tract catheterisation in patients undergoing vascular surgery in order to evaluate the efficiency of the routine prophylactic antibiotic treatment. | 1997 | 9267343 |
| molecular biology of mycoplasma. | mycoplasmas are the smallest free living microorganisms with the smallest genome. the g+c content is in general low (25-33%) and the coding capacity is about 600 proteins. mycoplasma species are phylogenetically related, they use the genetic codon uga for tryptophan, and show rapid evolution, with a high rate of divergence. the genomes of mycoplasma genitalium and mycoplasma pneumoniae have been fully sequenced. striking features of the m. genitalium sequencing project are the presence of a high ... | 1997 | 9286059 |
| genital mycoplasma infections. | since 1937, 13 mycoplasma species, two acholeplasma species, and one ureaplasma species have been isolated from humans. six of these have the urogenital tract as the primary site of colonisation but others, which have the oropharynx and respiratory tract as the primary site, are found occasionally in the urogenital tract because of orogenital contact. mycoplasma hominis was the first to be isolated and is most strongly associated with bacterial vaginosis (bv), together with a variety of other ba ... | 1997 | 9286063 |
| mycoplasma hominis and ureaplasma urealyticum in patients with sexually transmitted diseases. | mycoplasma hominis and ureaplasma urealyticum can be isolated with considerable frequency from the human urogenital tract and are thought to cause various syndromes such as nongonococcal urethritis, pelvic inflammatory disease, pyelonephritis or infertility. the aim of this study was the evaluation of the presence of different genital pathogens in patients with sexually transmitted diseases (std) and, in particular, the detection of mycoplasmas in individuals infected with genital microbes and a ... | 1997 | 9286064 |
| [bacterial flora of genital organs in women treated for infertility]. | bacterial flora in 72 women suffering from infertility was assessed. the medium age of women was 28.9 years (22-37) and the duration of infertility-5.5 years (2-15). cultures were taken during laparoscopy (65) or microsurgery (7) from vagina, cervical canal and the fallopian tubes. specific cultures for anaerobic and aerobic bacteria, n. gonorrhoeae, c. trachomatis and mycoplasma hominis were taken. in the specimens from fallopian tubes 21 species of bacteria were cultured. the most often coagul ... | 1997 | 9296942 |
| in-vitro susceptibilities of mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum to clinafloxacin, pd 131628, ciprofloxacin and comparator drugs. | 1997 | 9302005 | |
| detection of urogenital mycoplasmal infections in primates by use of polymerase chain reaction. | urogenital mycoplasmal infections could affect use of primates as models for reproductive system studies and could affect reproduction in captive primates, but could be useful as animal models of similar human infections. we conducted a pilot study to assess detection of urogenital mycoplasmal infections in primates by use of polymerase chain reaction (pcr). healthy animals were anesthetized, and vaginal, cervical, or endometrial and urethral swab specimens were collected from females and males, ... | 1997 | 9355087 |
| localized reversible frameshift mutation in an adhesin gene confers a phase-variable adherence phenotype in mycoplasma. | the variable adherence-associated (vaa) antigen of mycoplasma hominis is an abundant surface lipoprotein adhesin that may mediate important interactions of this wall-less prokaryotic pathogen with the human host. extensive mutational variation of vaa size, as well as sequence and antigenic divergence, has been described previously. using a series of clonal isolates representing an isogenic lineage of variants oscillating in vaa expression, vaa is further shown in this study to undergo high-frequ ... | 1997 | 9364912 |
| mycoplasma hominis pneumonia complicating bilateral lung transplantation: case report and review of the literature. | mycoplasma hominis is a commensal of humans. the organism has been predominantly associated with infections of the genitourinary tract. extragenital infections have been described in neonates, in women during the postpartum period, and in immunocompromised patients. pneumonia caused by m. hominis is very rare. this report describes the development of m. hominis pneumonia in a lung transplantation recipient and underscores the difficulty in establishing the correct diagnosis and the need for earl ... | 1997 | 9367488 |
| [the role of chlamydia trachomatis infections in women with urinary tract diseases]. | the aim of our study was to determine the incidence of chlamydia trachomatis (c. trachomatis) infections and its role in the etiopathogenesis of urinary tract infections in women. we studied 68 women, who where divided into two groups (i-with urethral syndrome, ii-with other urologic diseases). with the use of direct diagnostic methods (immunofluorescence method, tissue culture) c. trachomatis infection was found in 25/68 (36.8%) women. in the group of women with urethral syndrome the percentage ... | 1997 | 9377664 |
| induction of neutrophil chemoattractant cytokines by mycoplasma hominis in alveolar type ii cells. | bronchopulmonary dysplasia (bpd) is a chronic lung disease of premature infants who are mechanically ventilated due to respiratory distress. the disease consists of an initial inflammatory influx of neutrophils to the lungs, followed by long-term chronic fibrosis of the lung tissue. the antigenic repertoire that initiates the inflammatory component of bpd has not been defined. furthermore, the repertoire of cytokines responsible for attracting neutrophils to the lung and the mediators of pathoge ... | 1997 | 9393806 |
| a gtp-binding protein of mycoplasma hominis: a small sized homolog to the signal recognition particle receptor ftsy. | a protein homologous to the escherichia coli ftsy which in turn has characteristics in common with the alpha-subunit of the eukaryotic signal recognition particle receptor (sralpha) in the membrane of the endoplasmic reticulum, was identified in mycoplasma hominis and its encoding dna sequenced. the aa similarity to e. coli ftsy and b. subtilis ftsy was 38% and 51%, respectively. the protein was synthesized in e. coli, purified and shown to bind gtp. subcellular localization studies revealed tha ... | 1997 | 9409769 |
| septic arthritis by mycoplasma hominis: a case report and review of the medical literature. | 1997 | 9462180 | |
| characterization of mycoplasma hominis mutations involved in resistance to fluoroquinolones. | fluoroquinolone-resistant mutants of mycoplasma hominis were selected in vitro from the pg21 susceptible reference strain either by multistep selection on increasing concentrations of various fluoroquinolones or by one-step selection on agar medium with ofloxacin. the quinolone resistance-determining regions (qrdr) of the structural genes encoding the a and b subunits of dna gyrase were amplified by pcr, and the nucleotide sequences of eight multistep-selected resistant strains were compared to ... | 1997 | 9021178 |
| vaginal flora changes associated with mycoplasma hominis. | the aim of this study was to investigate any association between vaginal carriage of mycoplasma hominis and genital signs and symptoms, other microbial findings, and some risk behavior factors in women with and without bacterial vaginosis. | 1997 | 9024109 |
| [detecting ureaplasma urealyticum, mycoplasma hominis and mycoplasma genitalium in the urogenital tract]. | to epidemiological study of ureaplasma urealyticum (uu), mycoplasma hominis (mh) and mycoplasma genitalium (mg) in the urogenital tract of different groups of people. | 1997 | 9596936 |
| [inflammatory smears in cervicovaginal cytology. a finding meaning infection?]. | inflammatory changes are relatively frequent findings in cervical smears and are generally believed to be a consequence of genital infection. however, clinical signs of infection are frequently absent and no consensus exists on the management of these patients. the objective of this study was to assess whether or not inflammatory smears are exclusively a consequence of genital infection. for this purpose, the prevalence of genital infection in a group of women with inflammatory cells in the cerv ... | 1997 | 9477586 |
| [new data on the etiological factors in acute pyelonephritis]. | spectrum of microbe agents of acute pyelonephritis has been studied. it is shown that mycoplasma hominis plays an important role in etiology of acute pyelonephritis besides the already known enterobacteria and gram-positive cocci. it is also established that mycoplasmal pyelonephritis more often develops in patients with the diseases of urinary blade (cystitis) and genital organs. results of serological investigations which confirm etiological role of isolated microorganisms in the initiation of ... | 1997 | 9480016 |
| the in-vitro activity of grepafloxacin against chlamydia spp., mycoplasma spp., ureaplasma urealyticum and legionella spp. | the activity of grepafloxacin, a new orally active fluoroquinolone, was compared with the activities of ofloxacin, clarithromycin and doxycycline against chlamydia pneumoniae, chlamydia trachomatis, mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum, and with the activities of ofloxacin, clarithromycin and rifampicin against legionella spp. grepafloxacin (mic range 0.06-0.12 mg/l) was some 8-16 times more active than ofloxacin against the chlamydiae, showing activity similar to ... | 1997 | 9484871 |
| [presence of urogenital mycoplasma in fertile women]. | 1997 | 9522530 | |
| [detection and analysis of mycoplasma in 400 cases of genitourinary infection in wuhan area]. | mycoplasmas were examined by cultured in the urogenital secreta and the midpieceurine from 400 cases (88 men and 312 women) of genitourinary infection and 119 cases (positive rate 29.75%) of genitourinary infection in wuhan area. the positive rates of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) were 24.75% and 8.75% respectively. the authors also studied the infection rates of neisseria gonorrheae, fungus and trichomonad by cultures and microscopic findings. the positive rates of nei ... | 1997 | 9812505 |
| high rates of genital mycoplasma infection in the highlands of papua new guinea determined both by culture and by a commercial detection kit. | duplicate vaginal swabs were collected from 100 women, and comparisons were made between an in-house broth-agar culture system and a commercially available kit, the mycoplasma ist kit (biomérieux), for the detection of mycoplasma hominis and ureaplasma urealyticum. there was good agreement between the two systems for detection of the genital mycoplasmas in terms of sensitivity, with values of > 92% being obtained. in terms of specificity, the mutual comparisons were less favorable, though specif ... | 1997 | 8968907 |
| a fatal case of mycoplasma hominis meningoencephalitis in a full-term newborn. | we report the case of a 20-day-old full-term baby, born to a mother who had had an uncomplicated pregnancy and delivery, who died 13 days after the onset of meningitis. mycoplasma hominis was the sole agent repeatedly recovered from cerebrospinal fluid and from postmortem brain tissue. | 1997 | 8968928 |
| prevalence and antibiotic susceptibility of ureaplasma urealyticum in malaysian neonates with respiratory distress. | ureaplasma urealyticum was isolated from the endotracheal aspirates of 39 (21.4%) of 182 neonates with respiratory distress requiring ventilatory support. mycoplasma hominis was isolated from one (0.5%) neonate. bacterial cultures were negative in 123 (67.6%) neonates. antibiotic susceptibility test carried out on ten isolates of u. urealyticum showed that all the organisms were sensitive to erythromycin but resistant to lincomycin and sulfamethoxazole trimethoprim. all, except one, u. urealytic ... | 1997 | 10968119 |
| a review of the in-vitro activity of quinupristin/dalfopristin against intracellular pathogens and mycoplasmas. | quinupristin/dalfopristin displays in-vitro bacteriostatic activity against all legionella spp. (mics = 0.06-2 mg/l), with legionella pneumophila usually being at least two-fold more sensitive to quinupristin/dalfopristin than legionella bozemanii, legionella dumoffii, legionella gormanii and legionella micdadei (mic = 0.06-2 vs 1-2 mg/l, respectively). against legionella spp., quinupristin/dalfopristin was at least as active as erythromycin. quinupristin/dalfopristin was active in vitro against ... | 1997 | 9511064 |
| antibodies to mycoplasma fermentans in hiv-positive heterosexual patients: seroprevalence and association with aids. | there are conflicting reports concerning the prevalence of mycoplasma fermentans in hiv-positive patients and its association with aids. serum antibodies to m. fermentans were measured by a modified immunoblotting technique in 48 hiv-positive heterosexual patients and in 30 hiv-negative heterosexual controls. antibodies to m. fermentans were detected in 19 (40%) of hiv-positive patients and in three (10%) of the hiv-negative controls (p = 0.01). the prevalence of antibodies to mycoplasma hominis ... | 1998 | 9515674 |
| [detection of tetracycline- and erythromycin-resistant urogenital mycoplasma strains using pcr]. | the use of pcr made it possible to obtain data on the occurrence of genital mycoplasms in subjects with different pathological states of their urogenital system. the detection rate of u.urealyticum was 10.7-25.1% in males and 23.0-61.9% in females. the detection rate of mycoplasma hominis was 6.2-11.4% in males and 3.8-22.9% in females. the detection rate of m.genitalium was 6.2-29.6% in males and 4.7-30.0% in females. the study carried out by means of amplification test systems for the detectio ... | 1998 | 9949490 |
| [mycoplasma hominis and ureaplasma urealyticum of cervical canal and in the pouch of douglas in infertile women: a preliminary report]. | detection of mycoplasma hominis and ureaplasma urealyticum in the samples obtained from the cervical canal of the uterus and from the pouch of douglas in 71 women in whom underwent diagnostic and operative laparoscopy is presented. ureaplasma urealyticum in 33 cases diagnosed for infertility was present in 6 (18.2%) patients. in cervical canal and the pouch of douglas in 5 and 2 women respectively. in 1 (3.0%) woman ureaplasma urealyticum was present in both places. in the women from the control ... | 1998 | 10224793 |
| simultaneous detection of hpv and other sexually transmitted agents in chronic urethritis. | many pathogens may be responsible of non gonococcal urethritis (ngu) with the possible occurrence of symptomatic and asymptomatic mixed viral and bacterial infections. in particular, genital papillomaviruses (hpvs) have been searched since they are linked to both benign and malignant lesions of the penis and urethra and the presence of a potential male carried state has received limited scrutiny while the screening of sexually active females has received substantial attention. | 1998 | 9973807 |
| [use of a method of single-stranded dna fragment conformation polymorphism (sscp) for detecting resistance of mycoplasm hominis to fluoroquinolones]. | single-strand conformation polymorphism (sscp) method was used for nucleotide sequence variation analysis of the gyrase a subunit quinolone resistance determining region (gyra qrdr) in a laboratory and clinical strains of m. hominis. the couple of primers selected for this region amplified specific product in clinical material. m. hominis cultures growing in the presence of different concentrations of ciprofloxacin were studied by the sscp method. ser(83) to leu mutation described previously was ... | 1998 | 9987752 |
| [investigation of intrauterine microbes after intrauterine operation]. | to explore the postoperative changes in the cultures of ureaplasma urealyticum (uu), mycoplasma hominis (mh), l-form bacteria (l-form), anaerobic bacteria (ana) and chlamydia trochomatis (ct) after intrauterine operation. | 1998 | 10682488 |
| symptomatic bacteriuria due to ureaplasma and mycoplasma in adults. | 314 women, and 52 men were screened during 18 months, for the presence of ureaplasma urealyticum and mycoplasma hominis in a midstream specimens of urine. all the patients were adults with symptoms of urinary tract infection who consulted a physician at a primary health center "josé castro villagrana" in tlalpan, d.f. méxico. ureaplasma urealyticum was isolated in 54 (17.1%) of the women and 8 (15.4%) of the men. only escherichia coli and gardnerella vaginalis were isolated more frequently in bo ... | 1998 | 10932729 |
| rubella virus and chronic joint disease: is there an association? | synovial fluid samples and/or biopsies from 79 patients with various chronic inflammatory joint diseases or traumatic joint injury were tested for rubella virus (rv) in order to confirm or refute results from other studies that suggested rv as a cause of chronic inflammatory joint disease. sixty-eight of the 72 patients tested had rv antibodies. rv rna was detected by reverse transcription-pcr in the synovial fluid cells from two patients. rv was also isolated by cell culture from the synovial f ... | 1998 | 9817866 |
| [formation of m. hominis and a. laidlawii resistance to fluoroquinolones]. | mycoplasma hominis and acholeplasma laidlawii cultures resistant to antibacterial fluoroquinolone drugs ciprofloxacin (cpf), ofloxacin (ofl), and lomefloxacin (lmf) were prepared by selection in liquid nutrient medium with ascending concentrations of cpf. resistant mycoplasma clones contained point mutations in the gyrase. a gene region determining quinolone resistance (qrdr gyra): m. hominis contained c-->t transition resulting in substitution of ser(83) for leu and a. laidlawii g-->a resulting ... | 1998 | 9819821 |
| complications of abortion performed under local anesthesia. | to assess the incidence of complications of abortion performed under local anesthesia. | 1998 | 9846716 |
| truncation as a novel form of variation of the p50 gene in mycoplasma hominis. | a characteristic feature of the mycoplasmas is the presence of variable surface proteins which may play an important role in the adaptation of the cell-wall-less organisms to their host environments. in addition, this antigen variation may be an important pathogenic property of the organism. the ubiquity of the gene encoding p50, an adhesin of mycoplasma hominis fbg, and its transcription were analysed in different isolates of m. hominis. the p50 gene was present in all isolates tested. based on ... | 1998 | 9846733 |
| association of genital mycoplasma colonization with low birth weight. | this study was conducted to document the prevalence of maternal genital tract colonization by ureaplasma urealyticum and mycoplasma hominis, and to assess its association with low birth weight (lbw) and prematurity. | 1998 | 9856315 |
| mycoplasma hominis parasitism of trichomonas vaginalis. | 1998 | 9872279 | |
| antigenic and genomic homogeneity of successive mycoplasma hominis isolates. | sixty mycoplasma hominis isolates were obtained from the cervices of pregnant women and from the ears or pharynges of their newborn babies. the isolates were examined by sds-page and pulsed-field gel electrophoresis. antigenic and genomic profiles were obtained for 16 series with two or more successive isolates. both analyses led to the conclusion that isolates from the same woman were identical or nearly identical, while isolates from different women exhibited a high degree of variation with re ... | 1998 | 9877186 |
| two patients with premature labour associated with mycoplasma hominis infection. | because several reports have suggested that bacterial vaginosis causes premature labour and early rupture of the fetal membranes, the presence of a bacterial flora that causes bacterial vaginosis is thought to be a risk factor for premature labour. the present study investigated two patients with premature delivery and intra-uterine mycoplasma hominis infection. in microbiological studies, gram's staining of amniotic fluids revealed numerous neutrophils and epithelial cells but no micro-organism ... | 1998 | 9879962 |
| [rokitamycin in the treatment of female genital chlamydia and mycoplasma infections. comparative study vs josamycin ]. | the macrolides are among the most effective antibiotics against infections due to chlamydia and mycoplasma. the drug in such cases must have marked antibacterial activity, good oral bioavailability, and high intracellular diffusion--indispensable for instance with chlamydia infection. rokitamycin, a macrolide with a 16-atom lactone ring, has the features for use in the treatment of genital infections caused by chlamydia or mycoplasma, penetrating the cell and reaching considerably higher concent ... | 1998 | 9882991 |
| ureaplasma urealyticum and mycoplasma hominis isolation from cervical secretions of pregnant women and nasopharyngeal secretions of their babies at delivery. | a prospective study was carried out at the university hospital, kuala lumpur to determine the cervical carriage rate of ureaplasma urealyticum and mycoplasma hominis among healthy pregnant women at delivery and the incidence of nasopharyngeal colonisation among their infants. | 1998 | 9885690 |
| [survey of sexually transmitted diseases in the region of rio cuarto]. | sexually transmitted diseases (std) are acquired mainly through sexual intercourse, being one of the most frequent groups of infectious diseases worldwide and consequently an important public health problem. the aim of this paper was to determine the current state of std and to compare different diagnostic methods in the population studied. a total of 1060 samples from vaginal flows, endocervical material and urethral discharge were studied during 3 years. of the total samples, 583 were positive ... | 1998 | 9922478 |
| mycoplasma hominis expresses two variants of a cell-surface protein, one a lipoprotein, and one not. | a protein similar to the previously characterized variable surface-exposed membrane protein p120 was identified (p120'), establishing that mycoplasma hominis pg21 possesses a novel gene family. the gene, p120', was sequenced and found to have some distinctive properties including a putative start codon of gtg, rather than the common atg codon, and a coding region with a high g + c content, characteristic of essential housekeeping genes in mycoplasmas. no sequence homology was found to known prot ... | 1998 | 9534245 |
| few microorganisms associated with bacterial vaginosis may constitute the pathologic core: a population-based microbiologic study among 3596 pregnant women. | to evaluate the association between various microorganisms isolated from the genital tract in pregnant women with bacterial vaginosis. | 1998 | 9539529 |
| sequences of the 16s rrna genes and phylogeny of the goat mycoplasmas mycoplasma adleri, mycoplasma auris, mycoplasma cottewii and mycoplasma yeatsii. | the nucleotide sequences of the 16s rrna genes from the type strains of four goat mycoplasmas, mycoplasma adleri, mycoplasma auris, mycoplasma cottewii and mycoplasma yeatsii, were determined by direct solid-phase dna sequencing. polymorphisms were found in two of the 16s rrna gene sequences, showing the existence of two different rrna operons. three polymorphisms were found in m. adleri, and one was found in m. yeatsii. the sequence information was used for the construction of phylogenetic tree ... | 1998 | 9542096 |
| [severe genital mycoplasma infection following cesarean section]. | authors report a serious case of post-caesarean delivery endometritis caused, probably exclusively, by genital mycoplasmas: ureaplasma urealyticum and mycoplasma hominis. the initial treatment of the patient with various penicillins, ceftriaxone, gentamicin, metronidazole and nystatine proved ineffective. subsequently, as microbiological tests turned out positive for genital mycoplasmas, a therapy of doxycyclin was introduced and a full recovery could be attained. authors' experience is consiste ... | 1998 | 9545798 |
| microbiology of acute arthropathies among children in argentina: mycoplasma pneumoniae and hominis and ureaplasma urealyticum. | to describe the isolation of mycoplasmas and ureaplasmas from synovial fluid in pediatric patients with joint disorders. | 1998 | 9576384 |
| [appearance of inflammatory cytokines interleukin-1 beta and interleukin-6 in amniotic fluid during labor and in intrauterine pathogen colonization]. | subclinical intrauterine infections have been proposed to be one of the leading causes of preterm labor. the determination of inflammatory cytokines il-1 beta and il-6 in the amniotic fluid may be useful to identify women who have infection-associated preterm labor. amniotic fluid was collected from 99 women during amniocentesis, during cesarean section or at the time of amniotomy using sterile technique. il-1 beta and il-6 were determined by a specific elisa. fluid of each sample was cultured f ... | 1998 | 9577917 |
| vaginal flora changes associated with mycoplasma hominis. | 1998 | 9500509 | |
| therapy of bacterial vaginosis. | 1998 | 9511031 | |
| successful treatment of infection due to mycoplasma hominis with streptogramins in a renal transplant patient: case report and review. | 1998 | 9597262 | |
| elevated interleukin-8 concentrations in amniotic fluid of mothers whose neonates subsequently develop bronchopulmonary dysplasia. | to determine if an intrauterine sub-clinical inflammatory process is a risk factor for the development of bronchopulmonary dysplasia. | 1998 | 9605441 |
| influence of urogenital infections on sperm functions. | many studies have examined the impact of genital tract infections on male fertility; however, the effect of bacteriospermia on sperm quality is still controversial. bacterial infections are more frequently found in semen samples from asymptomatic infertile patients than in those from fertile men. bacteriospermia is also a common problem of male partners from couples undergoing ivf. therefore, the effects of microorganisms on human sperm acrosome reaction of oocytes have been studied in vitro and ... | 1998 | 9629446 |
| inhibition of human peripheral blood mononuclear cell proliferation by streptococcus pyogenes cell extract is associated with arginine deiminase activity. | streptococcus pyogenes (group a streptococcus) cell extracts (ce) have a remarkably powerful and dose-dependent inhibitory effect on antigen, superantigen, or mitogen-stimulated human peripheral blood mononuclear cell (pbmc) proliferation in vitro. purification of the inhibitory component present in s. pyogenes type m5 (manfredo strain) ce by anion-exchange chromatography followed by gel filtration chromatography showed that the inhibitor had an approximate native molecular mass of 100 kda. sodi ... | 1998 | 9632565 |
| a simple technique to isolate dna and supernatant of genital mycoplasma hominis and ureaplasma urealyticum. | vaginal colonization with mycoplasma hominis and ureaplasma urealyticum has been implicated as a cause of prematurity. several mechanisms to induce preterm labor have been discussed. the investigation of expressed and secreted enzymes requires a feasible method for culturing and further processing of these bacteria. we describe a simple technique for culturing of mycoplasma hominis and ureaplasma urealyticum without contamination with other microorganisms and isolating dna and supernatant. pcr a ... | 1998 | 9650134 |
| [infective endocarditis caused by unusual microorganisms]. | all series of infective endocarditis had a variable proportion of cases without an etiologic agent because all cultures were negative. new microbiologic techniques have permitted the discovery of the role of many microorganisms in infective endocarditis. c. burnetii is an increasing causative agent of subacute infective endocarditis. in the diagnosis, to the detection of antiphase-i antibodies, immunohistochemical, molecular techniques and cellular cultures have been added. total cure is difficu ... | 1998 | 9658953 |
| [catheter infection by mycoplasma hominis in a patient with acute lymphoblastic leukemia]. | 1998 | 9666596 | |
| the possible role of anaerobic bacteria in chronic prostatitis. | prostatis, the most common urological disease in men, afflicts between 25 and 50% of all adult men. four clinical categories are recognized: acute and chronic bacterial prostatitis, non-bacterial prostatitis and prostatodynia. the role of gram-positive aerobic bacteria and the different anaerobes in chronic bacterial prostatitis is still a matter of debate. during this study, the urethral discharge and the prostatic fluid obtained after prostatic massage of 50 patients with chronic prostatitis, ... | 1998 | 9669200 |
| chlamydia trachomatis: probable cause of prostatitis. | seventy-eight men with symptoms of chronic or subacute prostatitis were enrolled. investigations for the presence of chlamydia trachomatis in urethral swabs were carried out. the expressed prostatic secretions were additionally examined for mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, other gram-negative and gram-positive bacteria, trichomonas vaginalis, yeast-like fungi and leucocyte count. furthermore, all patients were evaluated for the presence of serum anti-chlamydial ... | 1998 | 9671250 |
| the in vitro simulation of mycoplasma hominis isolation using supplemented culture media in the bactec 9000 series blood culture system. | 1998 | 9684411 | |
| cloning and nucleotide sequences of the topoisomerase iv parc and pare genes of mycoplasma hominis. | the topoisomerase iv parc and pare genes from the wall-less organism mycoplasma hominis pg21 were cloned and sequenced. the coupled genes are located far from the dna gyrase genes gyra and gyrb. they encode proteins of 639 and 866 amino acids, respectively. as expected, the encoded pare and parc proteins exhibit higher homologies with the topoisomerase iv subunits of the gram-positive bacteria staphylococcus aureus and streptococcus pneumoniae than with their escherichia coli counterparts. the c ... | 1998 | 9687401 |
| the mycoplasma hominis vaa gene displays a mosaic gene structure. | mycoplasma hominis contains a variable adherence-associated (vaa) gene. to classify variants of the vaa genes, we examined 42 m. hominis isolated by pcr, dna sequencing and immunoblotting. this uncovered the existence of five gene categories. comparison of the gene types revealed a modular composition of the vaa proteins. the proteins constituted a conserved n-terminal part followed by a varying number of interchangeable cassettes encoding approximately 110 amino acids with conserved sequences b ... | 1998 | 9701806 |
| comparison of polymerase chain reaction assay with culture for detection of genital mycoplasmas in perinatal infections. | the polymerase chain reaction (pcr) technique was compared with culture for the detection of ureaplasma urealyticum, mycoplasma hominis, and mycoplasma genitalium in clinical samples (vaginal secretions, throat and endotracheal secretions, and skin swabs) obtained from 47 high-risk pregnant women peripartum and eight newborn infants. detection using pcr with homologous primers was highly specific, as a product with the expected length was consistently amplified with homologous but not with heter ... | 1998 | 9707308 |
| medical significance of mycoplasmas. | 1998 | 9711635 | |
| detection of mycoplasmas in urethral swabs from hiv-1 infected patients and control individuals using culture techniques and polymerase chain reaction. | the objective of the present study was to determine the prevalence of certain mycoplasma species, i.e., mycoplasma hominis, ureaplasma urealyticum and mycoplasma penetrans, in urethral swabs from hiv-1 infected patients compared to swabs from a control group. mycoplasmas were detected by routine culture techniques and by the polymerase chain reaction (pcr) technique, using 16srrna generic primers of conserved region and mycoplasma penetrans specific primers. the positivity rates obtained with th ... | 1998 | 9713129 |
| conservation of the amino-terminal epitope of elongation factor tu in eubacteria and archaea. | an epitope of elongation factor tu (ef-tu), which is found in organisms in both the bacterial and archaeal domains, was recently defined by mab 900. to localize the conserved epitope within the ef-tu molecule and to determine its sequence, spotscan analysis of synthetic peptides, western blot analysis of purified ef-tu domains and site-directed mutagenesis studies were used. analysis of mab 900 binding to overlapping 15-mer peptides encompassing the complete sequence of ef-tu of escherichia coli ... | 1998 | 9720046 |
| preterm birth: associations with genital and possibly oral microflora. | opportunistic pathogenic microbes are indigenous to the female lower genital tract and etiologic in many types of pelvic infections and, apparently, a portion of preterm birth (ptb) cases. bacterial vaginosis (bv) is a clinical syndrome based on an altered genital microflora in which gardnerella vaginalis; anaerobic species primarily among prevotella, porphyromonas, bacteroides, peptostreptococcus, and mobiluncus; mycoplasma hominis; and ureaplasma urealyticum become predominant in vaginal secre ... | 1998 | 9722706 |
| mycoplasma contamination of chlamydia pneumoniae isolates. | we examined 6 c. pneumonia isolates from the american type culture collection (atcc) and 2 finnish isolates for mycoplasma contamination. three of the atcc isolates and both of the finnish isolates were mycoplasma-contaminated. the contaminants were characterized by means of growth in bea and beg media, immunoblotting, polymerase chain reaction and pulsed field gel electrophoresis. two of the 6 atcc isolates [atcc vr1355 (twar strain 2043) and atcc vr1356 (twar strain 2023)] were infected with m ... | 1998 | 9730308 |
| dna sequencing reveals limited heterogeneity in the 16s rrna gene from the rrnb operon among five mycoplasma hominis isolates. | to investigate the intraspecies heterogeneity within the 16s rrna gene of mycoplasma hominis, five isolates with diverse antigenic profiles, variable/identical p120 hypervariable domains, and different 16s rrna gene rflp patterns were analysed. the 16s rrna gene from the rrnb operon was amplified by pcr and the pcr products were sequenced. three isolates had identical 16s rrna sequences and two isolates had sequences that differed from the others by only one nucleotide. | 1998 | 9734066 |
| alterations in topoisomerase iv and dna gyrase in quinolone-resistant mutants of mycoplasma hominis obtained in vitro. | mycoplasma hominis mutants were selected stepwise for resistance to ofloxacin and sparfloxacin, and their gyra, gyrb, parc, and pare quinolone resistance-determining regions were characterized. for ofloxacin, four rounds of selection yielded six first-, six second-, five third-, and two fourth-step mutants. the first-step mutants harbored a single asp426-->asn substitution in pare. gyra changes (ser83-->leu or trp) were found only from the third round of selection. with sparfloxacin, three round ... | 1998 | 9736554 |
| [detection using molecular biology techniques of mycoplasma hominis and ureaplasma urealyticum in urogenital samples]. | mycoplasma hominis and ureaplasma urealyticum are species closely related to urogenital diseases such as pyelonephritis, nongonococcal urethritis, urinary calculi, epididymitis, pelvic inflammation, infertility, abortions and post-delivery fever. they can also cause pneumonia and meningitis in newborn infants. in this paper we used nucleic acid hybridization and polymerase chain reaction to analyze 22 samples from patients with different urogenital symptoms in order to detect mycoplasmas and ure ... | 1998 | 9744030 |
| ultrasonographic assessment of the uterine cervix and interleukin-8 concentrations in cervical secretions predict intrauterine infection in patients with preterm labor and intact membranes. | interleukin-8 concentrations in cervical secretions have been related to microbial invasion of the amniotic cavity and histological chorioamnionitis. since a short uterine cervix may be a risk factor for intrauterine infection, we set out to determine the interrelationship between cervical-secretion interleukin-8 concentration, cervical length measured by transvaginal sonography and intrauterine infection in women with preterm labor and intact membranes. | 1998 | 9744050 |
| elevated amniotic fluid interleukin-6 as a predictor of neonatal periventricular leukomalacia and intraventricular hemorrhage. | > objective: to investigate the relationship between amniotic fluid interleukin-6 levels and the development of periventricular leukomalacia and intraventricular hemorrhage in the preterm neonate and to compare the value of amniotic fluid interleukin-6 with amniotic fluid culture and histologic chorioamnionitis in the prediction of periventricular leukomalacia and intraventricular hemorrhage. methods: 119 women, between 20 and 34 weeks gestation, in preterm labor with intact membranes, underwent ... | 1998 | 9745094 |
| absence of phospholipase a2 activity in genital mycoplasma hominis. | the clinical relevance of vaginal colonization with mycoplasma hominis (m hominis) as a cause of prematurity is doubtful. one of the possible pathways which could explain the role of m hominis in the induction of preterm labour is an increased synthesis of prostaglandins by a phospholipase a2 activity. the aim of this study was to prove whether m hominis secrets proteins with a pla2 activity and to test whether there are strain differences in the enzyme activity between m hominis isolated from w ... | 1998 | 9773381 |
| multiplex pcr for the detection of mycoplasma fermentans, m. hominis and m. penetrans in cell cultures and blood samples of patients with chronic fatigue syndrome. | a multiplex polymerase chain reaction (pcr) was initially developed to detect the presence of mycoplasma genus dna sequences in cell cultures and to differentiate between three human pathogenic mycoplasma species simultaneously. the assay in turn, proved to be a useful tool for the detection of mycoplasma infection in human dna samples. one set of oligonucleotide primers which are specific for a highly conserved region among all members of the genus mycoplasma along with three other primer sets ... | 1998 | 9778455 |
| mycoplasma hominis parasitism of trichomonas vaginalis. | 1998 | 9788469 | |
| dna analysis for phospholipase a2 coding sequences of mycoplasma hominis isolated from women with a normal pregnancy and women with a pregnancy complicated by preterm labour. | a possible cause of preterm labour is an increased synthesis of prostaglandins by a phospholipase a2 (pla2) activity. pla2 activity has been detected in mycoplasma hominis. the aim of this study was to test whether chromosomal dna of m. hominis contains sequences coding for pla2. m. hominis was cultured in specimens from 5 women with normal pregnancy and 4 in preterm labour. using sequence alignment, primer pairs for the active part of pla2 of different species were designed for pcr analysis. no ... | 1998 | 9789649 |
| cloning and expression of p60, a conserved surface-localized protein of mycoplasma hominis, in escherichia coli. | the clp60 gene encoding p60, a conserved lipoprotein of mycoplasma hominis, was cloned and sequenced from both the type strain pg21 and the isolate fbg. both open reading frames were identical in length, comprising 1746 nucleotides. the deduced amino acid sequences differed in 16 out of 582 amino acids. as expected, none of these divergences mapped within the epitope that was recognized by mab cg4 in all of the 198 isolates of m. hominis analyzed so far. this conserved epitope was narrowed down ... | 1998 | 9792448 |
| genital mycoplasmas stimulate tumor necrosis factor-alpha and inducible nitric oxide synthase production from a murine macrophage cell line. | ureaplasma urealyticum and mycoplasma hominis, two genital mycoplasmas, are the most common organisms isolated in the perinatal period and both either cause or are associated with poor perinatal outcomes. we speculate that these microbes could increase inflammation by stimulating macrophages to produce tumor necrosis factor-alpha (tnf-alpha) and inducible nitric oxide synthase because of their propensity to interact with the host's immune system. to test this hypothesis, raw 264.7 cells, a murin ... | 1998 | 9803463 |
| exogenous bovine surfactant suppresses tumor necrosis factor-alpha release by murine macrophages stimulated by genital mycoplasmas. | tumor necrosis factor-alpha (tnf-alpha) is a proinflammatory cytokine that appears to play a significant role in the development of neonatal chronic lung disease (cld). inflammation and cld are also associated with respiratory tract colonization with genital mycoplasmas. the possible protective roles of surfactant in mitigating the inflammatory response to these microbes were investigated. murine raw 264.7 macrophages were preincubated with an exogenous surfactant and exposed overnight to steril ... | 1998 | 9806043 |
| cytokine and prostaglandin production by amnion cells in response to the addition of different bacteria. | our goal was to evaluate the effect of escherichia coli, bacteroides fragilis, mycoplasma hominis, and staphylococcus aureus on cytokine and prostaglandin production by amnion cells in vitro. | 1998 | 9465802 |
| isolation of ureaplasma urealyticum and mycoplasma hominis from public toilet bowls. | the presence of ureaplasma urealyticum, mycoplasma hominis, and chlamydia trachomatis was explored in 50 public restroom toilet bowls. we used culture, antigen detection, polymerase chain reaction, and survival assay. five bowls (10%) were contaminated with at least one organism. u. urealyticum was found in four bowls, m. hominis in three, and c. trachomatis in one. u. urealyticum survived on the toilet rim for up to 2 hours. | 1999 | 9927273 |
| bacterial vaginosis a "broad overview". | bacterial vaginosis (bv) was first reported in 1995 by gardner and dukes, who described the unique clinical signs and symptoms and the distinctive nature of the vaginal discharge associated with it. they also described a "new" causative organism, which they named "haemophilus vaginalis", subsequently renamed gardnerella vaginalis. bv is currently the most prevalent cause of infectious vaginitis among women attending for genitourinary diseases. bv has a complex microbiology. lactobacillus populat ... | 1999 | 10932748 |
| mycoplasmal antibodies as determined with an enzyme-linked immunosorbent assay, in tubal factor infertility. | a total of 81 infertile women, who had been referred for diagnostic loparoscopy, were tested for the presence of antibodies to mycoplasma hominis and t-mycoplasma. out of 81, 30 had tubal adhesions and 51 had unilateral/bilateral tubal blockage. antibodies to m. hominis were found in 21/30 (70%) and 14/51 (27.45%) women, antibodies to t-mycoplasma in 12/20 (40% and 39/51 (76.47%) women with tubal disorder. in a control group of 40 pregnant women, antibodies to the same two organisms occurred in ... | 1999 | 10862270 |
| ureaplasma/mycoplasma-infected amniotic fluid: pregnancy outcome in treated and nontreated patients. | to determine if treatment of a positive amniotic fluid culture for mycoplasmal colonization obtained at genetic amniocentesis is associated with improved pregnancy outcome. | 1999 | 10685238 |
| multiple mycoplasmal infections detected in blood of patients with chronic fatigue syndrome and/or fibromyalgia syndrome. | the aim of this study was to investigate the presence of different mycoplasmal species in blood samples from patients with chronic fatigue syndrome and/or fibromyalgia syndrome. previously, more than 60% of patients with chronic fatigue syndrome/fibromyalgia syndrome were found to have mycoplasmal blood infections, such as mycoplasma fermentans infection. in this study, patients with chronic fatigue syndrome/fibromyalgia syndrome were examined for multiple mycoplasmal infections in their blood. ... | 1999 | 10691196 |
| [role of mutations in parc and gyra in forming resistance of mycoplasma hominis to fluoroquinolones]. | the set of the laboratory strain m. hominis h-34 mutants resistant to fluoroquinolones (ciprofloxacin-cfl, lomefloxacin-lfl, ofloxacin-ofl) was obtained by selection in broth medium. the mutation was found in the quinolone resistance-determining region (qrdr) of a subunit of topoisomerase iv gene (parc) and new mutations were found in qrdr of genes encoding the a subunit of dna gyrase (gyra) in m. hominis mutants resistant to various concentrations of the cfl, lfl and ofl. after multistep select ... | 1999 | 10621934 |
| [association of tap1/tap2 gene polymorphism with inflammatory urogenital diseases combined with joint diseases]. | to study association of tap1/tap2 gene polymorphism with urogenital infections combined with joint lesions. | 1999 | 10626334 |
| [the etiology of infectious cervicitis in women]. | infectious cervicitis is a common disease in women of reproductive age. a prospective study was carried out including 70 women with clinical manifestations of cervicitis during gynecological and colposcopic examination. in 19 women (27%), chlamydia trachomatis was demonstrated by direct immunofluorescence in cervical smears. neisseria gonorrhoeae was isolated in 2 women and 1 had a concomitant infection with neisseria gonorrhoeae and chlamydia trachomatis. mycoplasma hominis and ureaplasma ureal ... | 1999 | 10730379 |
| vaginal heat shock protein expression in symptom-free women with a history of recurrent vulvovaginitis. | the cause of recurrent vulvovaginitis remains unexplained in most cases. heat shock protein synthesis is induced under conditions of stress; its presence in vaginal samples from women who were between episodes of recurrent vulvovaginitis thus might reflect a persistent perturbation in the local milieu. study design: we undertook an evaluation by means of enzyme-linked immunosorbent assay of 60-kd heat shock protein and inducible 70-kd heat shock protein expressions in vaginal wash samples from 2 ... | 1999 | 10076122 |
| intravenous azithromycin. | to review the pharmacology, microbiology, chemistry, pharmacokinetics, efficacy, safety, tolerability, dosage, administration, and economic issues of intravenous azithromycin. | 1999 | 10084418 |
| [acute cystitis]. | 1999 | 10088462 | |
| mutations in the gyra, parc, and pare genes associated with fluoroquinolone resistance in clinical isolates of mycoplasma hominis. | five clinical isolates of mycoplasma hominis from three different patients were examined for resistance to fluoroquinolones; some of these isolates were probably identical. all five isolates harbored amino acid substitutions in the quinolone resistance-determining regions of both dna gyrase (gyra) and topoisomerase iv (parc or pare). furthermore, the novobiocin mic for three isolates showed a significant increase. this is the first characterization of fluoroquinolone-resistant clinical mycoplasm ... | 1999 | 10103208 |
| [mycoplasma hominis and ureaplasma urealyticum in different gynecologic diseases]. | the incidence of mycoplasma hominis (m. hominis) y ureaplasma urealyticum (u. urealyticum) was investigated in 113 endocervical samples obtained from women who were seen for different gynecological pathologies. forty-seven (42%) patients were positive to these microorganisms; 26 cases (23%) were positive for m. hominis and 21 (19%; p = ns) for u. urealyticum. average age was 32.1 +/- 7.7 years; the average number of sexual partners was 1.7 +/- 1.1. eleven of 17 patients with 3 o more sexual part ... | 1999 | 10198558 |
| mycoplasma penetrans and other mycoplasmas in urine of human immunodeficiency virus-positive children. | urine samples from children with human immunodeficiency virus (hiv) infection and healthy controls were examined for mycoplasmas by culture. standard biochemical assays, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and pcr (16s and 16s-23s spacer rrna region) were used for identification of isolates. mycoplasmas were identified from 13 (87%) of 15 hiv-positive patients and 3 (20%) of 15 hiv-negative control patients. the frequency and type of mycoplasma varied with the severity of ... | 1999 | 10203515 |
| the characterization of mycoplasma synoviae ef-tu protein and proteins involved in hemadherence and their n-terminal amino acid sequences. | an abundant cytoplasmic 43-kda protein from mycoplasma synoviae, a major pathogen from poultry, was identified as elongation factor tu. the n-terminal amino acid sequence (akldfdrskehvnvgtighv) has 90% identity with the sequence of the mycoplasma hominis elongation factor tu protein. monoclonal antibodies reacting with the m. synoviae elongation factor tu protein also reacted with 43-kda proteins from the avian mycoplasma species mycoplasma gallinarum, mycoplasma gallinaceum, mycoplasma pullorum ... | 1999 | 10220885 |
| cyto-adherence studies of the adhesin p50 of mycoplasma hominis. | recombinant peptides of mycoplasma hominis adhesin p50 were expressed in escherichia coli to investigate which regions of the p50 molecule are responsible for cyto-adherence. the respective dna fragments were obtained by pcr amplification of the p50 gene with the use of mutating oligonucleotides to change the tga codons of mycoplasma to tgg codons, which are translated in e. coli as tryptophan. the resulting three clones (i, i+ii and i+iii) contained regions of p50 which closely represent the re ... | 1999 | 10229546 |
| [pathogen spectrum of urethritis in the man]. | 545 male patients with a tentative diagnosis "urethritis" were examined between november 1984 and december 1994 in the department of dermatology and venerology of the military hospital in ulm. the patients, aged from 18 to 58 years (mean age 24.1 years), were examined according to a standardized diagnostic procedure: smear preparations from the urethra with subsequent gram staining, bacterial cultures for aerobic bacteria, neisseria gonorrhoeae (cultures and phadebact gonococcus test), mycoplasm ... | 1999 | 10231688 |