Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| existence of a hydrogen sulfide:ferric ion oxidoreductase in iron-oxidizing bacteria. | the existence of a hydrogen sulfide:ferric ion oxidoreductase, which catalyzes the oxidation of elemental sulfur with ferric ions as an electron acceptor to produce ferrous and sulfite ions, was assayed with washed intact cells and cell extracts of various kinds of iron-oxidizing bacteria, such as thiobacillus ferrooxidans 13598, 13661, 14119, 19859, 21834, 23270, and 33020 from the american type culture collection, leptospirillum ferrooxidans 2705 and 2391 from the deutsche sammlung von mikroor ... | 1992 | 16348640 |
| evaluation of leptospirillum ferrooxidans for leaching. | the importance of leptospirillum ferrooxidans for leach processes has been evaluated by studying the lithotrophic flora of three mine biotopes and a heap leaching operation, by percolation experiments with inoculated, sterilized ore, and by morphological, physiological, and genetic investigations of pure and mixed cultures of l. ferrooxidans, thiobacillus ferrooxidans, and thiobacillus thiooxidans. in biotopes of 20 degrees c or above, leptospirillum-like bacteria are as abundant as t. ferrooxid ... | 1992 | 16348642 |
| corrosion and electrochemical oxidation of a pyrite by thiobacillus ferrooxidans. | the oxidation of a pure pyrite by thiobacillus ferrooxidans is not really a constant phenomenon; it must be considered to be more like a succession of different steps which need characterization. electrochemical studies using a combination of a platinum electrode and a specific pyrite electrode (packed-ground-pyrite electrode) revealed four steps in the bioleaching process. each step can be identified by the electrochemical behavior (redox potentials) of pyrite, which in turn can be related to c ... | 1992 | 16348688 |
| molybdenum oxidation by thiobacillus ferrooxidans. | thiobacillus ferrooxidans ap19-3 oxidized molybdenum blue (mo) enzymatically. molybdenum oxidase in the plasma membrane of this bacterium was purified ca. 77-fold compared with molybdenum oxidase in cell extract. a purified molybdenum oxidase showed characteristic absorption maxima due to reduced-type cytochrome oxidase at 438 and 595 nm but did not show absorption peaks specific for c-type cytochrome. the optimum ph of molybdenum oxidase was 5.5. the activity of molybdenum oxidase was completel ... | 1992 | 16348710 |
| leaching of pyrites of various reactivities by thiobacillus ferrooxidans. | wide variations were found in the rate of chemical and microbiological leaching of iron from pyritic materials from various sources. thiobacillus ferrooxidans accelerated leaching of iron from all of the pyritic materials tested in shake flask suspensions at loadings of 0.4% (wt/vol) pulp density. the most chemically reactive pyrites exhibited the fastest bioleaching rates. however, at 2.0% pulp density, a delay in onset of bioleaching occurred with two of the pyrites derived from coal sources. ... | 1992 | 16348718 |
| enumeration of thiobacilli within ph-neutral and acidic mine tailings and their role in the development of secondary mineral soil. | the lemoine tailings of chibougamau, quebec, canada, were deposited as a ph-neutral mineral conglomerate consisting of aluminum-silicates, iron-aluminum-silicates, pyrite, chalcopyrite, and sphalerite. these tailings are colonized by an active population of thiobacillus ferrooxidans which is localized to an acid zone occupying 40% of the tailings' surface. this population peaked at 7 x 10 most probable number per gram of tailings during july and august 1990 and extended to a depth of 40 cm from ... | 1992 | 16348721 |
| anaerobic growth of thiobacillus ferrooxidans. | the obligately autotrophic acidophile thiobacillus ferrooxidans was grown on elemental sulfur in anaerobic batch cultures, using ferric iron as an electron acceptor. during anaerobic growth, ferric iron present in the growth media was quantitatively reduced to ferrous iron. the doubling time in anaerobic cultures was approximately 24 h. anaerobic growth did not occur in the absence of elemental sulfur or ferric iron. during growth, a linear relationship existed between the concentration of ferro ... | 1992 | 16348735 |
| oxidation of elemental sulfur to sulfite by thiobacillus thiooxidans cells. | thiobacillus thiooxidans cells oxidized elemental sulfur to sulfite, with 1 mol of o(2) consumption per mol of sulfur oxidized to sulfite, when the oxidation of sulfite was inhibited with 2-n-heptyl-4-hydroxyquinoline n-oxide. | 1992 | 16348814 |
| bioleaching of zinc sulfide concentrate by thiobacillus ferrooxidans. | the kinetics of the bioleaching of zns concentrate by thiobacillus ferrooxidans was studied in a well-mixed batch reactor. experimental studies were made at 30 degrees c and ph 2.2 on adsorption of the bacteria to the mineral, ferric iron leaching, and bacterial leaching. the adsorption rate of the bacteria was fairly rapid in comparison with the bioleaching rate, indicating that the bacterial adsorption is at equilibrium during the leaching process. the adsorption equilibrium data were correlat ... | 1992 | 18600888 |
| quantification of the intragranular porosity formed in bioleaching of pyrite by thiobacillus ferroxidans. | during the bacterial oxidation of a pure pyrite by thiobacillus ferrooxidans, a great number of corrosion tunnels appear that are easily revealed by scanning electron microscopy observations. this involves an increase in the surface area without significant granulometric reduction of mineral grains. thus, the evaluation of intragranular porosity, determined by elution front analysis, allows one to estimate accurately the fraction of oxidized sulphide, because of the development of deep holes (pr ... | 1992 | 18600913 |
| control of a thiobacillus denitrificans bioreactor using machine vision. | a pc-based machine vision system has been used to continuously monitor changes in biomass concentration and to control the undesirable production of colloidal elemental sulfur (a reactor upset condition due to an excessive concentration of inhibitory sulfide substrate) in a bioreactor containing thiobacillus denitrificans. a field of view of a video camera was established which contained regions of different background lighting. mean values of the distribution of red, green, and blue intensity c ... | 1992 | 18600979 |
| effect of applied potentials on the activity and growth of thiobacillus ferrooxidans. | the effect of applied dc potentials both in the positive and negative range, on the activity and growth of thiobacillus ferrooxidans, is discussed. in general, application of positive potentials up to +1000 mv in an acid bioleaching medium was found to be detrimental to bacterial activity, while the impression of negative potentials enhanced both their activity and growth through electrochemical regeneration of ferrous ions and an increase in the biomass. ferrous-ferric ratios in a bioleaching m ... | 1992 | 18601028 |
| dissolution of sulphur particles by thiobacillus ferrooxidans: substrate for unattached cells. | the growth of thiobacillus ferrooxidans on sulphur is known to proceed through the attachment of cells to the sulphur particles. experiments, however, show that the cells in the liquid phase, which are not attached to the sulphur particles, also grow. it has been shown through the use of a two-compartment membrane reactor that this increase is partially due to the release of ions, corresponding to partially oxidized of sulphur, into the solution by the attached cells. the main soluble ion has be ... | 1993 | 18609596 |
| mechanism of microbial flotation using thiobacillus ferrooxidans for pyrite suppression. | microbial desulfurization might be developed as a new process for the removal of pyrite sulfur from coal sluries such as coal-water mixture (cwm). an application of iron-oxidizing bacterium thiobacillus ferrooxidans to flotation would shorten the periods of the microbial removal of pyrite from some weeks by leaching methods to a few minutes. the floatability of pyrite in flotation was mainly reduced by t. ferrooxidans itself rather than by other microbial substances in bacterial culture as addit ... | 1993 | 18609604 |
| bacterial leaching of metals from sewage sludge by indigenous iron-oxidizing bacteria. | bioleaching of metals can be achieved in sewage sludge using thiobacillus ferrooxidans, which obtains its energy requirements from the oxidation of added ferrous iron. the purpose of this study was to verify the presence of indigenous t. ferroxidans and to evaluate their adaptive capacity and leaching potential. nineteen sludges (primary, secondary, aerobically and anaerobically digested, oxidation pond) were tested and all of them contained indigenous iron-oxidizing bacteria. the acclimation of ... | 1993 | 15091793 |
| examination of lipopolysaccharide (o-antigen) populations of thiobacillus ferrooxidans from two mine tailings. | net acid-generating capacities of 39.74 kg of h(2)so(4) per ton (ca. 0.05 kg/kg) (ph 2.68) for the lemoine copper mine tailings (closed ca. 8 years ago; located 40 km west of chibougamau, quebec, canada) and 16.07 kg of h(2)so(4) per ton (ca. 0.02 kg/kg) (ph 3.01) for the copper rand tailings (in current use and 50 km distant [east] from those of lemoine) demonstrate that these sulfide tailings can support populations of acidophilic thiobacilli. oxidized regions in both tailings environments wer ... | 1993 | 16348925 |
| mineral products of pyrrhotite oxidation by thiobacillus ferrooxidans. | the biological leaching of pyrrhotite (fe(1-x)s) by thiobacillus ferrooxidans was studied to characterize the oxidation process and to identify the mineral weathering products. the process was biphasic in that an initial phase of acid consumption and decrease in redox potential was followed by an acid-producing phase and an increase in redox potential. elemental s was one of the first products of pyrrhotite degradation detected by x-ray diffraction. pyrrhotite oxidation also yielded k-jarosite [ ... | 1993 | 16348977 |
| coal depyritization by the thermophilic archaeon metallosphaera sedula. | the kinetics of pyrite oxidation by metallosphaera sedula were investigated with mineral pyrite and two coals with moderate (pittsburgh no. 8) and high (new brunswick, canada) pyritic sulfur content. m. sedula oxidized mineral pyrite at a greater rate than did another thermophile, acidianus brierleyi, or a mesophile, thiobacillus ferrooxidans. maximum rates of coal depyritization were also greater with m. sedula, although the magnitude of biological stimulation above abiotic rates was notably le ... | 1993 | 16349006 |
| selective adhesion of thiobacillus ferrooxidans to pyrite. | bacterial adhesion to mineral surfaces plays an important role not only in bacterial survival in natural ecosystems, but also in mining industry applications. selective adhesion was investigated with thiobacillus ferrooxidans by using four minerals, pyrite, quartz, chalcopyrite, and galena. escherichia coli was used as a control bacterium. contact angles were used as indicators of hydrophobicity, which was an important factor in the interaction between minerals and bacteria. the contact angle of ... | 1993 | 16349106 |
| surface chemistry of thiobacillus ferrooxidans relevant to adhesion on mineral surfaces. | thiobacillus ferrooxidans cells grown on sulfur, pyrite, and chalcopyrite exhibit greater hydrophobicity than ferrous ion-grown cells. the isoelectric points of sulfur-, pyrite-, and chalcopyrite-grown cells were observed to be at a ph higher than that for ferrous ion-grown cells. microbe-mineral interactions result in change in the surface chemistry of the organism as well as that of the minerals with which it has interacted. sulfur, pyrite, and chalcopyrite after interaction with t. ferrooxida ... | 1993 | 16349107 |
| profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rrna. | we describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. this technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16s rrna, all the same length, by denaturing gradient gel electrophoresis (dgge). dgge analysis of different microbial communities demonstrated the presence of up to 10 distinguishable bands in the separation pattern, which were most likely derived from as many different species con ... | 1993 | 7683183 |
| identification of the pura gene encoding adenylosuccinate synthetase in thiobacillus ferrooxidans. | the pura gene of thiobacillus ferrooxidans encoding adenylosuccinate synthetase [ec 6.3.4.4] was identified in the upstream region of the iro gene encoding fe(ii)-oxidase (j. biol. chem 267:11242-11247, 1992). the pura gene consisted of 1290 base-pairs, which translated into a 29-amino-acid protein. the gene is functionally active, because it is able to complement an escherichia coli pura-deficient strain. the deduced gene product has a high degree (60.9%) of sequence identity with that (432 aa) ... | 1993 | 7763499 |
| transformation of the acidophilic heterotroph acidiphilium facilis by electroporation. | we constructed a cloning vector for use in the acidophilic heterotroph acidiphilium facilis. the vector pah101 (8.8 kb) was constructed from a 6.1 kb restriction fragment of the acidiphilium plasmid pah1 and a puc19 carrying a beta-lactamase gene. the antibiotic resistance gene was efficiently expressed in a. facilis. several factors which influenced the transformation efficiency were optimized, resulting in a transformation efficiency of up to 3 x 10(3) transformants per microgram of plasmid dn ... | 1993 | 7764274 |
| n-terminal heterogeneity of methylamine dehydrogenase from thiobacillus versutus. | the n-terminal processing of madh from the bacterium t. versutus and the n-terminal heterogeneity of the isolated alpha subunit of the alpha 2 beta 2 protein complex was demonstrated by a combination of edman sequence analysis of an electroblotted band, in situ digested with pyroglutamate aminopeptidase, and accurate mass determination of the homogeneous subunit by the technique of electrospray ionisation mass spectrometry. from this study, it appears that the corresponding gene of the alpha sub ... | 1993 | 7901050 |
| mutagenesis of the conserved lysine 14 of cytochrome c-550 from thiobacillus versutus affects the protein structure and the electron self-exchange rate. | the lysine residue k14 of cytochrome c-550 of thiobacillus versutus has been mutated to a glutamine (q) and a glutamate (e) residue. these mutations have a minimal effect on the pka for replacement of the methionine ligand (the "alkaline transition"), indicating that a presumptive salt bridge between k14 and e11 does not help stabilize the native form. this is in contrast with mitochondrial cytochrome c, where the homologous k13 forms a structurally important salt bridge with glutamate 90. the n ... | 1993 | 7903553 |
| bacterial catalytic processes for transformation of metals. | microorganisms actively participate in the transformation of metals and metalloids by various processes including adsorption, absorption, alkylation, oxidation and reduction reactions. bacteria of the genera thiobacillus and sulfolobus have a chemolithotrophic mode of metabolism and catalyze various metal transformations. these bacteria are primarily involved in oxidation-reduction reactions of metals. metal sulfides can be either directly oxidized by the microbes or oxidized by ferric iron, an ... | 1993 | 8181951 |
| amino acid sequence of a high redox potential ferredoxin (hipip) from the purple phototrophic bacterium rhodopila globiformis, which has the highest known redox potential of its class. | rhodopila globiformis hipip has a redox potential (ca. 450 mv) that is 100 mv higher than any other known iron-sulfur protein. the amino acid sequence contains 57 residues and can be aligned with that of thiobacillus ferrooxidans without any insertions or deletions and is 51% identical. rp. globiformis hipip is also similar to that of rhodocyclus tenuis, but six- and two-residue gaps must be postulated and there is only 37% identity. most of the amino acid residues near the iron-sulfur cluster a ... | 1993 | 8215406 |
| parallel induction of nitric oxide and glucose-6-phosphate dehydrogenase in activated bone marrow derived macrophages. | the production of nitric oxide (no.) and the induction of glucose-6-phosphate dehydrogenase by lipopolysaccharides (lps) from different sources was studied in bone marrow derived macrophages (bmm phi). no. production was found to be linked to the induction of glucose-6-phosphate dehydrogenase, suggesting the possible involvement of this enzyme in the cytotoxic mechanism resulting from the release of no. by activated macrophages. | 1993 | 8216310 |
| 3-isopropylmalate dehydrogenase from chemolithoautotroph thiobacillus ferrooxidans: dna sequence, enzyme purification, and characterization. | 3-isopropylmalate dehydrogenase encoded by the thiobacillus ferrooxidans leub gene was purified to homogeneity from escherichia coli cells harboring a recombinant plasmid containing the leub gene. the native enzyme molecule is a dimer of molecular weight 38,000. the km value for 3-isopropylmalate was estimated to be 26 microm and that for nad+ 0.8 mm. the presence of k+ or nh4+ is essential for the enzyme reaction. the enzyme is activated about 4-fold by the addition of 1.0 mm mg2+ or co2+. the ... | 1993 | 8282728 |
| a new mechanism for the aerobic catabolism of dimethyl sulfide. | aerobic degradation of dimethyl sulfide (dms), previously described for thiobacilli and hyphomicrobia, involves catabolism to sulfide via methanethiol (ch3sh). methyl groups are sequentially eliminated as hcho by incorporation of o2 catalyzed by dms monooxygenase and methanethiol oxidase. h2o2 formed during ch3sh oxidation is destroyed by catalase. we recently isolated thiobacillus strain asn-1, which grows either aerobically or anaerobically with denitrification on dms. comparative experiments ... | 1993 | 8285684 |
| aerobic and anaerobic degradation of a range of alkyl sulfides by a denitrifying marine bacterium. | a pure culture of a bacterium was obtained from a marine microbial mat by using an anoxic medium containing dimethyl sulfide (dms) and nitrate. the isolate grew aerobically or anaerobically as a denitrifier on alkyl sulfides, including dms, dimethyl disulfide, diethyl sulfide (des), ethyl methyl sulfide, dipropyl sulfide, dibutyl sulfide, and dibutyl disulfide. cells grown on an alkyl sulfide or disulfide also oxidized the corresponding thiols, namely, methanethiol, ethanethiol, propanethiol, or ... | 1993 | 8285707 |
| amplification of ribulose biphosphate carboxylase/oxygenase large subunit (rubisco lsu) gene fragments from thiobacillus ferrooxidans and a moderate thermophile using polymerase chain reaction. | southern blot analysis of dna from an iron-oxidising moderate thermophile nmw-6 and from thiobacillus ferrooxidans strain tfi-35 demonstrated sequences homologous to the rubisco lsu gene of synechococcus. dna fragments (457 bp) encoding part of the rubisco lsu gene (amino acids 73-200) were amplified from the genomic dna of thiobacillus ferrooxidans and the moderate thermophile nmw-6 using the polymerase chain reaction (pcr) technique (saiki et al. (1985) science 233, 1350-1354). a comparison wi ... | 1993 | 8357616 |
| enzymes of aerobic respiration on iron. | bacteria capable of aerobic respiration on ferrous ions are spread throughout eubacterial and archaebacterial phyla. comparative spectroscopic analyses revealed that phylogenetically distinct organisms expressed copious quantities of spectrally distinct redox-active biomolecules during autotrophic growth on soluble iron. thiobacillus ferroxidans, leptospirillum ferrooxidans, sulfobacillus thermosulfidooxidans, and metallosphaera sedula possessed iron respiratory chains dominated by a blue copper ... | 1993 | 8357617 |
| [analysis of restriction samples of chromosomal dna from strains of thiobacillus ferrooxidans by pulse electrophoresis]. | 1993 | 8371723 | |
| cloning and sequencing of the gene coding for the large subunit of methylamine dehydrogenase from thiobacillus versutus. | the gene that codes for the alpha-subunit of methylamine dehydrogenase from thiobacillus versutus, mada, was cloned and sequenced. it codes for a protein of 395 amino acids preceded by a leader sequence of 31 amino acids. the derived amino acid sequence was confirmed by partial amino acid sequencing. the start of the mature protein could not be determined by direct sequencing, since the n terminus appeared to be blocked. instead, it was determined by electrospray mass spectrometry. confirmation ... | 1993 | 8407797 |
| specific binding of thiobacillus ferrooxidans rbcr to the intergenic sequence between the rbc operon and the rbcr gene. | the presence of two sets (rbcl1-rbcs1 and rbcl2-rbcs2) of rbc operons has been demonstrated in thiobacillus ferrooxidans fe1 (t. kusano, t. takeshima, c. inoue, and k. sugawara, j. bacteriol. 173:7313-7323, 1991). a possible regulatory gene, rbcr, 930 bp long and possibly translated into a 309-amino-acid protein, was found upstream from the rbcl1 gene as a single copy. the gene is located divergently to rbcl1 with a 144-bp intergenic sequence. as in the cases of the chromatium vinosum rbcr and a ... | 1993 | 8432695 |
| amino-acid sequence of rusticyanin from thiobacillus ferrooxidans and its comparison with other blue copper proteins. | rusticyanin, a copper protein characterized by a high redox potential (+680 mv) and a high stability at acidic ph, is involved in iron oxidation in thiobacillus ferrooxidans. it has been characterized from a new strain and its amino-acid sequence has been determined and compared to two other rusticyanin sequences isolated from different strains. it comprises 155 amino acids and the alignment of the three rusticyanins shows a high degree of homology. comparing the rusticyanins with six blue coppe ... | 1993 | 8448191 |
| cloning and expression of the d-ribulose-1,5-bisphosphate carboxylase/oxygenase form ii gene from thiobacillus intermedius in escherichia coli. | both form i and ii ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) genes were detected in thiobacillus intermedius by heterologous hybridization using specific probes from anacystis nidulans and rhodobacter sphaeroides, respectively. however, only the previously reported form i enzyme could be demonstrated in cells grown under a number of different conditions. the reason(s) why the form ii gene is not expressed in t. intermedius is/are not clear at this time. the form ii gene was isola ... | 1993 | 8472910 |
| phosphate-starvation induced changes in thiobacillus ferrooxidans. | we have analysed the response of the acidophilic chemolithotroph thiobacillus ferrooxidans to phosphate starvation. cultivation of the bacteria in the absence of added phosphate induced a remarkable filamentation of the cells. polyacrylamide gel electrophoresis revealed several proteins whose levels increased upon phosphate limitation, as well as some polypeptides that were exclusively synthesized under this growth limitation. one of the proteins whose level increased by the lack of phosphate wa ... | 1993 | 8472923 |
| transfer of thiosphaera pantotropha to paracoccus denitrificans. | comparative sequence analysis of in vitro-amplified 16s rrna genes of thiosphaera pantotropha gb17t (t = type strain) and paracoccus denitrificans lmg 4218t revealed identical 16s rrna primary structures for the two organisms. the level of overall dna similarity of thiosphaera pantotropha gb17t and p. denitrificans dsm 65t is 85%, as determined by quantitative dna-dna hybridization. therefore, we propose the transfer of thiosphaera pantotropha to p. denitrificans. the closest relative of thiosph ... | 1993 | 8494744 |
| effect of ph on sulfite oxidation by thiobacillus thiooxidans cells with sulfurous acid or sulfur dioxide as a possible substrate. | the oxidation of sulfite by thiobacillus thiooxidans was studied at various ph values with changing concentrations of potassium sulfite. the optimal ph for sulfite oxidation by cells was a function of sulfite concentrations, rising with increasing substrate concentrations, while that by the cell extracts was unaffected. the sulfite oxidation by cells was inhibited at high sulfite concentrations, particularly at low ph values. the results from kinetic studies show that the fully protonated form o ... | 1994 | 8300544 |
| plasmid and transposon transfer to thiobacillus ferrooxidans. | the broad-host-range incp plasmids rp4, r68.45, rp1::tn501, and pub307 were transferred to acidophilic, obligately chemolithotrophic thiobacillus ferrooxidans from escherichia coli by conjugation. a genetic marker of kanamycin resistance was expressed in t. ferrooxidans. plasmid rp4 was transferred back to e. coli from t. ferrooxidans. the broad-host-range incq vector pjrd215 was mobilized to t. ferrooxidans with the aid of plasmid rp4 integrated in the chromosome of e. coli sm10. pjrd215 was st ... | 1994 | 8188590 |
| [application of immunologic methods to the analysis of bio-leaching bacteria]. | pure cultures of thiobacillus ferrooxidans and mixed cultures of thiobacillus ferrooxidans and leptospirillum ferrooxidans isolated from the matahambre mine (cuba) were used to fit immunodiffusion and immunoelectron microscopy to the study of iron oxidizing bacteria. the possibilities, advantages and limits of those techniques have been studied from both the identification and the serological characterization points of view. finally, the efficiency of these methods was tested by applying them to ... | 1994 | 7873106 |
| [preservation of acidophilic bacteria]. | 1994 | 7873107 | |
| preliminary study of treatment of sulphuric pickling water waste from steelmaking by bio-oxidation with thiobacillus ferrooxidans. | this report looks at the laboratory-scale recovery of iron oxides (alpha fe2o3 type) through bio-oxidation with thiobacillus ferrooxidans of the ferrous sulphate contained in steel industry sulphuric pickling liquors. this is done by calcining iron sulphates and iron and ammonium sulphates obtained from the crystallization of the oxidized solution. the products of the bacterial reaction and the iron oxides are then studied according to calcination temperature. the process carried out produced 50 ... | 1994 | 7917427 |
| the effects of ph and cations on the spectral and kinetic properties of methylamine dehydrogenase from thiobacillus versutus. | the catalytic parameters of thiobacillus versutus methylamine dehydrogenase (madh) with the physiological substrates methylamine and amicyanin show a ph profile that is quite different from the one found in commonly used assays with artificial electron acceptors. the optimum at ph 7.5, observed for kcat in the latter case, is absent with amicyanin as the reoxidizing substrate. with amicyanin kcat scarcely depends on ph; the same is true for the maximal rate of reduction of madh by methylamine (k ... | 1994 | 7918441 |
| identification and sequence analysis of the soxb gene essential for sulfur oxidation of paracoccus denitrificans gb17. | the coding region for lithotrophic sulfur oxidation (sox) in paracoccus denitrificans gb17 was identified by isolation of a transposon tn5-mob mutant with a sox- phenotype (strain tp19). the corresponding wild-type region was cloned previously (g. mittenhuber, k. sonomoto, m. egert, and c. g. friedrich, j. bacteriol. 173:7340-7344, 1991). sequence analysis of a 2.5-kb subclone that complemented strain tp19 revealed that tn5-mob was inserted into a coding region for a 553-amino-acid polypeptide n ... | 1994 | 7928987 |
| isolation and characterization of a carboxysome shell gene from thiobacillus neapolitanus. | the gene coding for the major carboxysome shell peptide (csos1) from thiobacillus neapolitanus has been isolated and sequenced. oligonucleotide primers for polymerase chain reaction (pcr) amplification of the 5' end of the gene were made possible by amino acid sequencing of the n-terminal residues of the shell peptide. a 41 bp pcr product was used as a probe to isolate the gene. the deduced amino acid composition of the 216 bp gene shows a high degree of hydrophobicity. the gene is located withi ... | 1994 | 7934888 |
| active site geometry in the high oxido-reduction potential rusticyanin from thiobacillus ferrooxidans. | rusticyanin is a blue copper protein involved in the oxidation of iron catalyzed by thiobacillus ferrooxidans. this protein is characterized by a high oxido-reduction potential and a high stability at low ph. the three dimensional structure of this protein is still unknown and in order to investigate the geometric properties of the copper center which could be correlated to the high oxido-reduction potential, we have studied rusticyanin by uv-visible, epr and nmr spectroscopies, at different ph ... | 1994 | 7945314 |
| biocorrosion produced by thiobacillus-like microorganisms. | biocorrosion can be produced by many different microorganisms through diverse mechanisms. the biocorrosion produced by acidophilic microorganisms of the genus thiobacillus is based on the production of sulfuric acid and ferric ion from pyrites or related mineral structures, as a result of the chemolithotrophic metabolism of these microorganisms. the products of this aerobic respiration are also powerful oxidant elements, which can produce chemical oxidations of other metallic structures. the tin ... | 1994 | 7946115 |
| [the endotoxins of gram-negative bacteria: their structure and biological role]. | main attention in the paper is paid to the study of lipid a, a component possessing endotoxic activity. lipids a containing glucosamine disaccharide (representatives of enterobacteriaceae family), and variants of lipid a differing from the toxic one either in the structure of carbohydrate core or in the spectrum of fatty acids are considered. they are either phototrophic, nodulating (bradyrhyzobium species) or soil species (nitrobacter and thiobacillus) bacteria. lipid a from lipopolysaccharides ... | 1994 | 7952230 |
| the f1 genes of the f1f0 atp synthase from the acidophilic bacterium thiobacillus ferrooxidans complement escherichia coli f1 unc mutants. | an atp gene cluster from the extreme acidophile thiobacillus ferrooxidans was able to complement escherichia coli f1 unc mutants for growth on minimal medium plus succinate. complementation with all four e. coli f1 mutants tested was observed and subunits for the f1 portion of the t. ferrooxidans atp synthase formed a functional association with the f0 subunits of the e. coli enzyme. in addition, a hybrid f1 enzyme in which some units were derived from e. coli and some from t. ferrooxidans was p ... | 1994 | 7958772 |
| nuclear magnetic resonance 15n and 1h resonance assignments and global fold of rusticyanin. insights into the ligation and acid stability of the blue copper site. | nuclear magnetic resonance assignments are reported at ph approximately 3 for a type 1 ("blue") copper protein, rusticyanin, obtained from the acidophilic organism thiobacillus ferrooxidans. a combination of homonuclear proton and heteronuclear 15n-edited nmr spectra has been used to assign most of the 1h and 15n resonances of reduced rusticyanin. the copper-binding site is shown by analogy with other blue copper proteins to contain the side-chains of cys138, his143 and met148 at the c-terminal ... | 1994 | 7990128 |
| isolation of the thiobacillus ferrooxidans ntrbc genes using a t. ferrooxidans nifh-lacz fusion. | an agar plating technique was developed in which the activation of expression of a thiobacillus ferrooxidans nifh-lacz gene fusion was used to isolate the ntrbc genes from a t. ferrooxidans gene library. an escherichia coli ntrc mutant containing the nifh-lacz fusion was transformed and plated on a low-nitrogen medium so that on flooding with onpg, the production of yellow colonies indicated the presence of the cloned t. ferrooxidans ntrbc genes. a 4.47 kb region from the t. ferrooxidans chromos ... | 1994 | 8000526 |
| kinetics of the reduction of wild-type and mutant cytochrome c-550 by methylamine dehydrogenase and amicyanin from thiobacillus versutus. | to elucidate the kinetic properties of the methylamine dehydrogenase (madh) redox chain of thiobacillus versutus the reduction of cytochrome c-550 by madh and amicyanin has been studied. under steady state conditions, the rate constants of the reactions have been determined as a function of the ionic strength, both for wild type cytochrome c-550 and for mutants in which the conserved residue lys14 has been replaced as follows: lys14-->gln (mutant [k14q]cytochrome c-550) and lys14-->glu (mutant [ ... | 1994 | 8020493 |
| genetic organization of the mau gene cluster in methylobacterium extorquens am1: complete nucleotide sequence and generation and characteristics of mau mutants. | the nucleotide sequence of the methylamine utilization (mau) gene region from methylobacterium extorquens am1 was determined. open reading frames for 11 genes (maufbedacjglmn) were found, all transcribed in the same orientation. the maub, maua, and mauc genes encode the periplasmic methylamine dehydrogenase (madh) large and small subunit polypeptides and amicyanin, respectively. the products of maud, maug, maul, and maum were also predicted to be periplasmic. the products of mauf, maue, and maun ... | 1994 | 8021187 |
| solution structure of the type 1 blue copper protein amicyanin from thiobacillus versutus. | a three-dimensional solution structure of amicyanin from thiobacillus versutus has been determined by distance geometry and restrained molecular dynamics. a total of 984 experimentally derived constraints were used for the final refinement (881 distance constraints and 103 dihedral angle constraints). stereospecific assignments were made for 17 prochiral beta-methylene protons (33%) and the methyl groups of eight valine residues. fourteen structures were selected to represent the solution struct ... | 1994 | 8035459 |
| two membrane-bound c-type cytochromes of thiobacillus ferrooxidans: purification and properties. | membrane-bound cytochrome c, cytochrome c-552 (m) was purified from thiobacillus ferrooxidans. it showed an absorption peak at 410 nm in the oxidized form, and peaks at 552, 523 and 416 nm in the reduced form. its molecular mass, em,7 and isoelectric point were 22,300, +0.336 volt and 9.1, respectively. another membrane-bound cytochrome c, cytochrome c-550 (m) was also purified. it showed an absorption peak at 408 nm in the oxidized form, and peaks at 550, 523 and 418 nm in the reduced form. its ... | 1994 | 8039654 |
| characterization of is1201, an insertion sequence isolated from lactobacillus helveticus. | is1201, a 1387-bp insertion sequence isolated from lactobacillus helveticus, was identified by its nucleotide (nt) sequence. it carries a single open reading frame encoding a 369-amino-acid protein, which shares homology with transposases found in a class of related is, including isrm3 from rhizobium meliloti, is256 from staphylococcus aureus, is6120 from mycobacterium smegmatis, is1081 from m. bovis, ist2 from thiobacillus ferroxidans and is406 from pseudomonas cepacia. is1201 has terminal inve ... | 1994 | 8045427 |
| respiratory enzymes of thiobacillus ferrooxidans. kinetic properties of an acid-stable iron:rusticyanin oxidoreductase. | rusticyanin is an acid-stable, soluble blue copper protein found in abundance in the periplasmic space of thiobacillus ferrooxidans, an acidophilic bacterium capable of growing autotrophically on soluble ferrous sulfate. an acid-stable iron:rusticyanin oxidoreductase activity was partially purified from cell-free extracts of t. ferrooxidans. the enzyme-catalyzed, iron-dependent reduction of the rusticyanin exhibited three kinetic properties characteristic of aerobic iron oxidation by whole cells ... | 1994 | 8049223 |
| transposition of ist2 in thiobacillus ferrooxidans. | the genome of thiobacillus ferrooxidans contains at least two different repetitive dna elements. one of these elements, termed ist2 has been sequenced and shown to exhibit the characteristics of a typical prokaryotic insertion sequence. furthermore, preliminary evidence has implicated ist2 in genomic rearrangements, although the mechanism of rearrangement, whether by transposition or recombination, has not been established. in this report we provide evidence from detailed restriction enzyme anal ... | 1994 | 8057836 |
| characterization of mutant met100lys of cytochrome c-550 from thiobacillus versutus with lysine-histidine heme ligation. | the heme iron in cytochrome c-550 from thiobacillus versutus has a methionine and a histidine as axial ligands. in order to study the characteristics of a possible lysine-histidine ligation in a heme protein, the methionine has been replaced by a lysine. this residue acts as a ligand between ph 3 and 12. the midpoint potential of the mutant has shifted -329 mv compared to wild type, but apart from this shift the ph dependence of the midpoint potential is unchanged, suggesting that the large drop ... | 1994 | 8060974 |
| the role of lysine 99 of thiobacillus versutus cytochrome c-550 in the alkaline transition. | the methionine ligand of the heme iron in ferricytochrome c-550 from thiobacillus versutus is replaced by another residue at high ph. this transition is similar to the alkaline transition in mitochondrial cytochrome c. to investigate the possible role of lysine 99 in this process, this residue has been mutated to a glutamate. the mutation causes the apparent pka of the transition to decrease from 11.2 in wild type to 10.8 in lys99glu cytochrome c-550. this destabilization of the native form is a ... | 1994 | 8076674 |
| purification and characterization of two new c-type cytochromes involved in fe2+ oxidation from thiobacillus ferrooxidans. | two new c-type cytochromes have been purified from cell membranes of the acidophilic thiobacillus ferrooxidans. in contrast to a soluble cytochrome c with molecular mass of 14 kda reported earlier, a membrane-bound cytochrome c with a mass of 21 kda was solubilized with octylthioglucoside and purified to homogeneity. in addition, a high molecular mass c-type cytochrome (68 kda) was also solubilized and purified using triton x-100 as a detergent. both acid-stable species are partially released du ... | 1994 | 8082827 |
| crystal structure analysis and refinement at 2.15 a resolution of amicyanin, a type i blue copper protein, from thiobacillus versutus. | the crystal structure of the type i blue copper protein amicyanin from thiobacillus versutus has been determined by patterson search techniques on the basis of the molecular model of amicyanin from paracoccus denitrificans, and refined by energy-restrained least-squares methods. amicyanin crystallizes in the trigonal space group p3(2) with unit cell dimensions of a = b = 87.40 a, c = 38.20 a. the asymmetric unit is composed of three independent molecules centred on the crystallographic 3(2) axes ... | 1994 | 8120896 |
| transfer and expression of degradative and antibiotic resistance plasmids in acidophilic bacteria. | the genetic accessibility of selected acidophilic bacteria was investigated to evaluate their applicability to degrading pollutants in acidic environments. the incp1 antibiotic resistance plasmids rp4 and pvk101 and the phenol degradation-encoding plasmid ppgh11 were transferred from neutrophilic bacteria into the extreme acidophilic eubacterium acidiphilium cryptum at frequencies of 1.8 x 10(-2) to 9.8 x 10(-4) transconjugants per recipient cell. the incq antibiotic resistance plasmid psup106 w ... | 1994 | 8161188 |
| molecular genetics of thiobacillus ferrooxidans. | thiobacillus ferrooxidans is a gram-negative, highly acidophilic (ph 1.5 to 2.0), autotrophic bacterium that obtains its energy through the oxidation of ferrous iron or reduced inorganic sulfur compounds. it is usually dominant in the mixed bacterial populations that are used industrially for the extraction of metals such as copper and uranium from their ores. more recently, these bacterial consortia have been used for the biooxidation of refractory gold-bearing arsenopyrite ores prior to the re ... | 1994 | 8177170 |
| biological production and consumption of gaseous organic sulphur compounds. | 1994 | 7698397 | |
| isolation and study of two strains of leptospirillum-like bacteria from a natural mixed population cultured on a cobaltiferous pyrite substrate. | two strains of leptospirillum-like bacteria, l6 and l8, have been isolated from a mixed inoculum, also containing thiobacillus ferrooxidans and t. thiooxidans, cultured for one year with a colbaltiferous pyrite as energy substrate in a 100 l continuous bioleaching laboratory unit. several physiological properties of the strains are described. the vibrio-shaped microorganisms grew at ph values lower than 1.3. their growth rate was maximum between 2.5 and 8.0 g l1 ferrous iron. the optimal growth ... | 1994 | 7710275 |
| stability of copper tolerance in thiobacillus ferrooxidans. | a strain of thiobacillus ferrooxidans mal-4-1 was adapted to grow at higher concentrations of copper by repeated subculturing in the presence of increasing levels of added cupric ions in 9k medium. the strains adapted to copper were found to be more efficient in bioleaching of copper from concentrates. when copper tolerant strains were back cultured repeatedly in 9k medium without cupric ions, the initially developed metal tolerance was observed to be lost. this indicates that the copper toleran ... | 1994 | 7710276 |
| characterisation and amino acid sequence of cytochrome c-550 from thiosphaera pantotropha. | a cytochrome c-550, with mid-point potential +265 mv, has been purified from thiosphaera pantotropha. the cytochrome was recognised by antibodies to paracoccus denitrificans cytochrome c-550, but the two proteins were not immunologically identical. amino acid sequencing of the cytochrome c-550 showed 85.9% and 95.5% identities, respectively, with the cytochromes c-550 of p. denitrificans and thiobacillus versutus; these are amongst the highest values reported for similarities between class i c-t ... | 1994 | 7508392 |
| cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments. | a range of autotrophic and heterotrophic enrichment cultures were established to determine the cultural bacterial diversity present in samples obtained from the acidic runoff of a chalcocite overburden heap and from laboratory-scale (1- to 4-liter) batch and continuous bioreactors which were being used for the commercial assessment of the bioleachability of zinc sulfide ore concentrates. strains identified as thiobacillus ferrooxidans, thiobacillus thiooxidans, "leptospirillum ferrooxidans," and ... | 1994 | 7517131 |
| thiobacillus ferrooxidans tyrosyl-trna synthetase functions in vivo in escherichia coli. | the tyrosyl-trna synthetase gene (tyrz) from thiobacillus ferrooxidans, an acidophilic, autotrophic, gram-negative bacterium that participates in bioleaching of minerals, was cloned and sequenced. the encoded polypeptide (tyrrz) is 407 amino acids in length (molecular mass; 38 kda). the predicted protein sequence has an extensive overall identity (44%) to the sequence of the protein encoded by the bacillus subtilus tyrz gene, one of the two genes encoding tyrosyl-trna synthetases in this microor ... | 1994 | 7517395 |
| phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of roseococcus thiosulfatophilus gen. nov., sp. nov., erythromicrobium ramosum gen. nov., sp. nov., and erythrobacter litoralis sp. nov. | we analyzed the 16s ribosomal dnas of three obligately aerobic, bacteriochlorophyll a-containing bacteria, "roseococcus thiosulfatophilus," "erythromicrobium ramosum," and new isolate t4t (t = type strain), which was obtained from a marine cyanobacterial mat. "roseococcus thiosulfatophilus" is a member of the alpha-1 subclass of the proteobacteria and is moderately related to rhodopila globiformis, thiobacillus acidophilus, and acidiphilium cryptum (level of sequence similarity, 90%). "erythromi ... | 1994 | 7520734 |
| the structure of the o-specific polysaccharide from thiobacillus ferrooxidans ifo 14262. | lipopolysaccharide (lps) was isolated from thiobacillus ferrooxidans ifo 14262 by the hot phenol-water extraction procedure. the o-specific polysaccharide, liberated from lps by mild acetic acid hydrolysis, had a branched pentasaccharide repeating-unit composed of d-glucose, l-rhamnose, d-rhamnose, and 3-o-methyl-l-rhamnose in approximate molar ratios of 2:1:1:1. on the basis of methylation analysis, 1h and 13c nmr spectroscopy, including 2d shift-correlated (cosy) and 1d noe spectroscopy, the s ... | 1994 | 7522125 |
| characterization of thiobacillus caldus sp. nov., a moderately thermophilic acidophile. | two isolates of a novel, moderately thermophilic thiobacillus species have been studied. the isolates, ku and bc13, are gram-negative, motile bacteria having a ph optimum for growth of 2-2.5 and an optimum growth temperature of 45 degrees c. both isolates are capable of chemolithotrophic growth on reduced sulfur substrates. they can also use molecular hydrogen as an electron donor. these two isolates can grow mixotrophically with sulfur or tetrathionate and yeast extract or glucose. the g+c cont ... | 1994 | 7533596 |
| sensitivity of iron-oxidizing bacteria, thiobacillus ferrooxidans and leptospirillum ferrooxidans, to bisulfite ion. | when grown on iron-salt medium supplemented with the bisulfite ion, leptospirillum ferrooxidans was much more sensitive to the ion than was thiobacillus ferrooxidans. the causes of the sensitivity of l. ferrooxidans to the bisulfite ion were studied. the bisulfite ion completely inhibited the iron-oxidizing activities of l. ferrooxidans and t. ferrooxidans at 0.02 and 0.2 mm, respectively. a trapping reagent for the bisulfite ion, formaldehyde, completely reversed the inhibition. the treatment o ... | 1994 | 16349199 |
| expression of heterogenous arsenic resistance genes in the obligately autotrophic biomining bacterium thiobacillus ferrooxidans. | two arsenic-resistant plasmids were constructed and introduced into thiobacillus ferrooxidans strains by conjugation. the plasmids with the replicon of wide-host-range plasmid rsf1010 were stable in t. ferrooxidans. the arsenic resistance genes originating from the heterotroph were expressed in this obligately autotrophic bacterium, but the promoter derived from t. ferrooxidans showed no special function in its original host. | 1994 | 16349341 |
| enhanced yields of iron-oxidizing bacteria by in situ electrochemical reduction of soluble iron in the growth medium. | an electrochemical apparatus for culturing chemolithotrophic bacteria that respire aerobically on ferrous ions is described. enhanced yields of the bacteria were achieved by the in situ electrochemical reduction of soluble iron in the growth medium. when subjected to a direct current of 30 a for 60 days, a 45-liter culture of thiobacillus ferrooxidans grew from 6 x 10 to 9.5 x 10 cells per ml. growth of the bacterium within the electrolytic bioreactor was linear with time. a final cell density c ... | 1994 | 16349344 |
| partial removal of lipopolysaccharide from thiobacillus ferrooxidans affects its adhesion to solids. | conditions for the partial removal of lipopolysaccharide (lps) from thiobacillus ferrooxidans are described. raising the ph of the solution containing the cells from ph 1.5 to ph 6.8 to 8.0 releases about 50% of the lps without cell lysis. the release of lps begins at ph 3.5, and it was not affected by edta concentration. partial removal of lps exposed higher amounts of a 40-kda outer membrane protein in the bacteria, as detected by a dot immunoassay employing an antiserum against the t. ferroox ... | 1994 | 16349352 |
| oxidative dissolution of arsenopyrite by mesophilic and moderately thermophilic acidophiles. | the purpose of this work was to determine solution- and solid-phase changes associated with the oxidative leaching of arsenopyrite (feass) by thiobacillus ferrooxidans and a moderately thermoacidophilic mixed culture. jarosite [kfe(3)(so(4))(2)(oh)(6)], elemental sulfur (s), and amorphous ferric arsenate were detected by x-ray diffraction as solid-phase products. the oxidation was not a strongly acid-producing reaction and was accompanied by a relatively low redox level. the x-ray diffraction li ... | 1994 | 16349379 |
| solubilization of minerals by bacteria: electrophoretic mobility of thiobacillus ferrooxidans in the presence of iron, pyrite, and sulfur. | thiobacillus ferroxidans is an obligate acidophile that respires aerobically on pyrite, elemental sulfur, or soluble ferrous ions. the electrophoretic mobility of the bacterium was determined by laser doppler velocimetry under physiological conditions. when grown on pyrite or ferrous ions, washed cells were negatively charged at ph 2.0. the density of the negative charge depended on whether the conjugate base was sulfate, perchlorate, chloride, or nitrate. the addition of ferric ions shifted the ... | 1994 | 16349387 |
| an immunological assay for detection and enumeration of thermophilic biomining microorganisms. | a specific, fast, and sensitive nonradioactive immunobinding assay for the detection and enumeration of the moderate thermophile thiobacillus caldus and the thermophilic archaeon sulfolobus acidocaldarius was developed. it employs enhanced chemiluminescence or peroxidase-conjugated immunoglobulins in a dot or slot blotting system and is very convenient for monitoring thermophilic bioleaching microorganisms in effluents from industrial bioleaching processes. | 1994 | 16349398 |
| desulfurization of coal by microbial column flotation. | twenty-three strains capable of oxidizing iron were isolated from coal and ore storage sites as well as coal and ore mines, volcanic areas, and hot spring. four strains were found to have high iron-oxidizing activity. one strain (t-4) was selected for this experiment since the strain showed the fastest leaching rate of iron and sulfate from pyrite among the four strains. the t-4 strain was assigned for thiobacillus ferrooxidans from its cultural and morphological characteristics.bacterial treatm ... | 1994 | 18618455 |
| kinetics of growth and elemental sulfur oxidation in batch culture of thiobacillus ferrooxidans. | the kinetics of oxidation of elemental sulfur by thiobacillus ferrooxidans in a batch reactor was followed by measuring the concentration of adsorbed cells on the sulfur surface, the concentration of free cells in liquid medium, and the amount of sulfur oxidized. as the elemental sulfur was oxidized to sulfate, the liquid-phase concentration of free cells continued to increase with time, whereas the surface concentration of adsorbed cells per unit weight of sulfur approached a limiting value, i. ... | 1994 | 18618826 |
| occurrences at mineral-bacteria interface during oxidation of arsenopyrite by thiobacillus ferrooxidans. | the combination of an improved bacterial desorption method, scanning electron microscopy (sem), diffuse reflectance and transmission infrared fourier transform spectroscopy, and a desorption-leaching device like high-pressure liquid chromatography (hplc) was used to analyze bacterial populations (adhering and free bacteria) and surface-oxidized phases (ferric arsenates and elemental sulfur) during the arsenopyrite biooxidation by thiobacillus ferrooxidans. the bacterial distribution, the physico ... | 1995 | 18623257 |
| biological sulphide oxidation in a fed-batch reactor. | this study shows that, in a sulphide-oxidizing bioreactor with a mixed culture of thiobacilli, the formation of sulphur and sulphate as end-products from the oxidation of sulphide can be controiledinstantaneously and reversibiy by the amount of oxygen supplied. it was found that at sulphide loading rates of up to 2.33 mmol7/l . h, both products can be formed already at oxygen concentrations below 0.1 mg/l. because the microorganisms tend to form sulphate rather than forming sulphur, the oxygen c ... | 1995 | 18623408 |
| bioleaching of pyrite by acidophilic thermophile acidianus brierleyi. | the kinetics of bioleaching of pyrite (fes(2)) by the acidophilic thermophilic bacterium acidianus brierleyi was studied in a well-mixed batch reactor. experiments were done at 65 degrees c and ph 1.5 on adsorption of a. brierleyi onto pyrite particles, liquid-phase oxidation of ferrous iron by a. brierleyi, and microbial leaching of pyrite. the adsorption of a. brierleyi was a fast process; equilibrium was attained within the first 30 min of exposure to pyrite. the adsorption equilibrium data w ... | 1995 | 18623527 |
| microbial diversity in uranium mine waste heaps. | two different uranium mine waste heaps near ronneburg, thuringia, germany, which contain the remains of the activity of the former uranium-mining soviet-east german company wismut ag, were analyzed for the occurrence of lithotrophic and chemoorganotropic leach bacteria. a total of 162 ore samples were taken up to a depth of 5 m. cell counts of ferrous iron-, sulfur-, sulfur compound-, ammonia-, and nitrite-oxidizing bacteria were determined quantitatively by the most-probable-number technique. s ... | 1995 | 16535096 |
| use of electroporation to generate a thiobacillus neapolitanus carboxysome mutant. | two cloning vectors designed for use in escherichia coli and the thiobacilli were constructed by combining a thiobacillus intermedius plasmid replicon with a multicloning site, lacz(prm1), and either a kanamycin or a streptomycin resistance gene. conditions necessary for the introduction of dna into t. intermedius and t. neapolitanus via electroporation were examined and optimized. by using optimal electroporation conditions, the gene encoding a carboxysome shell protein, csos1a, was insertional ... | 1995 | 16535117 |
| growth kinetics of thiobacillus thiooxidans on the surface of elemental sulfur. | the growth kinetics of thiobacillus thiooxidans on elemental sulfur in batch cultures at 30(deg)c and ph 1.5 was studied by measuring the time courses of the concentration of adsorbed cells on sulfur, the concentration of free cells suspended in liquid medium, and the amount of sulfur oxidized. as the elemental sulfur was oxidized to sulfate ions, the surface concentration of adsorbed cells per unit mass of sulfur approached a maximum value (maximum adsorption capacity of sulfur particles) where ... | 1995 | 16535145 |
| characterization of the ptfi91-family replicon of thiobacillus ferrooxidans plasmids. | plasmids found in six strains of thiobacillus ferrooxidans were mapped and compared in an effort to detect the origin of replication. four strains yielded an identical 9.8-kb plasmid, ptfi91. restriction mapping and southern blot hybridization analysis were used to confirm this finding. dissimilar plasmids found in two other strains contained a conserved 2.2-kb saci region common to ptfi91. dna sequence analysis of this region showed structural features common to bacterial plasmid replicons. a c ... | 1995 | 8590413 |
| molecular aspects of the electron transfer system which participates in the oxidation of ferrous ion by thiobacillus ferrooxidans. | the enzymes and redox proteins, which participate in the oxidation of ferrous ion by the acidophilic iron-oxiding bacterium thiobacillus ferrooxidans, have been isolated and characterized. they are fe(ii)-cytochrome c oxidoreductase, cytochromes c-552(s), c-552(m) and c-550(m), rusticyanin, and cytochrome c oxidase. on the basis of the interactions of these components, an electron transfer system has been proposed which seems to function in the oxidation of ferrous ion by the bacterium. | 1995 | 8845189 |
| [use of polymerase chain reaction for detecting the rbpc gene in natural samples]. | a method was developed allowing detection of the ribulose bisphosphate carboxylase (rubisco)-encoding gene in natural samples. the method is based on polymerase chain reaction (pcr) in the presence of oligonucleotide primers complementary to the conserved fragments of the rbcl gene from oxy- and anoxygenic phototrophic bacteria and some chemoautotrophic bacteria. use of this method made it possible to detect the rbcl gene in samples of natural water and sediments from antarctic lakes of the bung ... | 1995 | 8643038 |
| the structure of the o-specific polysaccharide from thiobacillus sp. ifo 14570, with three different diaminopyranoses forming the repeating unit. | the o-specific polysaccharide, liberated by mild acid hydrolysis of the lipopolysaccharide (lps-p) from thiobacillus sp. ifo14570 as isolated from the phenol phase after phenol-water extraction, is shown to have a linear trisaccharide repeating-unit containing three different diamino sugars, namely 2,4-diacetamido-2,4-dideoxyglucuronic acid, 2-acetamidino-4-acetamido-2,4,6-trideoxyglucopyranose, and 2,3-diacetamido-2,3-dideoxyglucuronic acid in the molar ratio of 1:1:1. on the basis of 1h and 13 ... | 1995 | 7539718 |
| paracoccus thiocyanatus sp. nov., a new species of thiocyanate-utilizing facultative chemolithotroph, and transfer of thiobacillus versutus to the genus paracoccus as paracoccus versutus comb. nov. with emendation of the genus. | a facultatively chemolithotrophic thiocyanate-degrading bacterium, strain thi 011t, which was previously isolated from activated sludge and tentatively named thiobacillus sp., was studied taxonomically and phylogenetically. this bacterium utilizes thiocyanate as sole energy source and the specific growth rate for chemolithoautotrophic growth with thiocyanate was 0.059 h-1. molecular phylogenetic relationships of strain thi 011t to thiobacillus versutus and members of the genus paracoccus were el ... | 1995 | 7545513 |
| evidence for a methylammonium-binding site on methylamine dehydrogenase of thiobacillus versutus. | the nonconvertible substrate analogues di-, tri-, and tetramethylammonium are bound with fairly high affinity to oxidized methylamine dehydrogenase (madhox) from thiobacillus versutus and induce the same red-shift in the optical absorbance spectrum of madhox as do the monovalent cations cs+, rb+, and nh4+. like the monovalent cations, trimethylamine also competitively inhibits the reduction of madhox by methylamine. rapid-scan experiments show that within the first few milliseconds of the reacti ... | 1995 | 7548050 |
| x-ray absorption studies and homology modeling define the structural features that specify the nature of the copper site in rusticyanin. | rusticyanin, a blue copper protein, possessing the highest redox potential among this class of proteins and a high stability at acidic ph reveals homology with the c-terminal end of the other single copper containing blue proteins and an interesting homology to parts of the blue copper domain of the multi-copper proteins such as the nitrite reductases. extended x-ray absorption fine structure (exafs) data at ph 2.0 reveal that cu is ligated to two his and a cys in the inner coordination sphere, ... | 1995 | 7599131 |
| gene synthesis, high-level expression, and mutagenesis of thiobacillus ferrooxidans rusticyanin: his 85 is a ligand to the blue copper center. | an artificial gene of the blue copper protein rusticyanin from thiobacillus ferrooxidans was constructed from eight overlapping oligonucleotides in a recursive "one-pot" polymerase chain reaction. the gene was placed behind the t7/lacor promoter of pet24a and expressed in escherichia coli as a soluble protein. a purification scheme involving a ph titration step, cation-exchange chromatography, and reverse-phase hplc separation provided yields of the apoprotein ranging from 70 to 100 mg/l of cell ... | 1995 | 7639845 |
| identification and activity of two insertion sequence elements in rhodococcus sp. strain igts8. | two putative insertion sequence (is) elements, is1166 and is1295, were identified on a plasmid present in rhodococcus sp. igts8. four copies of is1166 were present in strain igts8: one copy on each of two separate plasmids and two copies on a third plasmid or in the chromosome. of eight rhodococci tested, only r. zopfii contained a copy of an is1166-like element. two mutants of strain igts8 were isolated in which an additional copy of is1166 was present, suggesting that at least one copy of this ... | 1995 | 7642133 |