Publications

acute disseminated encephalomyelitis after rocky mountain spotted fever.	although acute disseminated encephalomyelitis has been observed after a variety of viral infections and an occasional bacterial infection, it has not been reported in association with rickettsial infections. reported is a 7-year-old male with magnetic resonance images and clinical manifestations suggestive of acute disseminated encephalomyelitis after a tick bite and serologically proven rocky mountain spotted fever.	1999	10428440
characterization of monoclonal antibodies to an immunodominant protein of the etiologic agent of human granulocytic ehrlichiosis.	immunodominant proteins in the range of 42-45 kd are important for the serodiagnosis of human granulocytic ehrlichiosis (hge). antigens from human isolates of the etiologic agent of hge cultivated in hl-60 cells were used to immunize balb/c mice and generate a panel of hybridomas secreting monoclonal antibodies. using an enzyme immunoassay, an immunofluorescent assay (ifa), and western blotting, we showed that culture supernatants and ascites of these hybridomas were reactive with human isolates ...	1999	10432075
platelet-associated immunoglobulin (antiplatelet antibody) in canine rocky mountain spotted fever and ehrlichiosis.	antiplatelet antibodies were detected in the sera of dogs with naturally occurring and experimentally induced rickettsia rickettsii and ehrlichia canis infections. this is the first known report documenting elevated platelet-associated immunoglobulin (paig) titers in rocky mountain spotted fever (rmsf) infections. in the naturally occurring rmsf infections and ehrlichiosis, the antibodies persisted for weeks or months, even when the platelet counts had normalized. results of this study indicate ...	1999	9934930
family cluster of rocky mountain spotted fever.	soon after a patient from tennessee died of rocky mountain spotted fever (rmsf), several family members developed symptoms suggestive of the disease and were treated presumptively for rmsf. fifty-four persons visiting the index patient's home were interviewed; serum samples were collected from 35. three additional cases of rmsf were confirmed, all of which occurred in first-degree relatives. time spent at the family home and going into the surrounding woods were significantly associated with dev ...	1999	10825050
pituitary and ovarian responses to luteinizing-hormone-releasing hormone during pregnancy and after parturition in brown hares (lepus europaeus).	female hares were given an i.v. injection of 5 micrograms luteinizing-hormone-releasing hormone (lhrh) between days 7 and 19 (n = 21), 20 and 33 (n = 17) and 34 and 41 (n = 17) of pregnancy, and in the 3 days after parturition (n = 16). whatever the stage of pregnancy, the lhrh injection induced a release of luteinizing hormone (lh) and follicle-stimulating hormone (fsh) and an acute secretion of progesterone; these hormonal responses increased significantly during pregnancy, to reach values sim ...	1999	1905354
[protector of our body].		1999	22082
[beta-2 microglobulin in pathology. general review and personal results].		1999	85424
interphase studies with a simplified method of silver staining of nucleoli.	a simple silver staining method is presented providing a rapid and reliable technique for the selective staining of nuclear structures synthesizing ribosomal rna (18s and 28s rna).	1999	84766
serum alphafetoprotein (afp) in bilharziasis.	two hundred and eighty-four patients of various ages from an endemic area, diagnosed as having bilharziasis by the presence of living schistosoma haematobium's ova in the mid-stream urine sample, have been investigated for the presence of alphafetoprotein (afp) in the serum. one hundred and forty-one (49.6%), were positive; no false positives have occurred. radioimmunoassay is capable of increasing the positivity rate still further. the detection of afp will be of limited value as a screening te ...	2000	89881
recent advances in the immunodiagnosis of cancer.		2000	87017
drain-cover injuries in children.	this report highlights unnecessary injuries to the hands, forearms, and feet of small children, caused by falling drain-cover gratings, colloquially known as "shores". it is suggested that a simple locking device, similar to that employed on manhole covers, would eliminate this type of injury.	2000	14764
meningoencephalitis secondary to bacterial otitis media/interna in a dog.	central nervous system (cns) complications of bacterial otitis media/interna are an infrequent occurrence in human patients and have rarely been reported in the veterinary literature. early recognition of cns involvement and the use of appropriate diagnostic tests to characterize the nature of the lesion(s) are crucial in determining the best course of treatment. in this paper, the authors describe a dog with bacterial meningoencephalitis secondary to otitis media/interna.	2000	10825096
in vitro susceptibilities of rickettsia and bartonella spp. to 14-hydroxy-clarithromycin as determined by immunofluorescent antibody analysis of infected vero cell monolayers.	the in vitro susceptibilities of rickettsia akari, rickettsia conorii, rickettsia prowazekii, rickettsia rickettsii, bartonella elizabethae, bartonella henselae and bartonella quintana to different concentrations of clarithromycin, 14-hydroxy-clarithromycin (the primary metabolite of clarithromycin) and tetracycline in vero cell cultures, were determined by enumeration of immunofluorescently-stained bacilli. the extent of antibiotic-induced inhibition of foci was recorded for each dilution of an ...	2000	10702548
post-transcriptional regulation of endothelial cell plasminogen activator inhibitor-1 expression during r. rickettsii infection.	intracellular infection of endothelial cells with rickettsia rickettsii results in increased steady-state levels of plasminogen activator inhibitor-1 (pai-1) mrna. control mechanisms governing such increased expression in response to this novel stimulus have not been defined. in this study, we compared the stability of pai-1 mrna in infected and uninfected endothelial cells (ec) and explored the requirement for de novo host cell protein synthesis in the infection-induced increase of steady-state ...	2000	10702354
consequences of delayed diagnosis of rocky mountain spotted fever in children--west virginia, michigan, tennessee, and oklahoma, may-july 2000.	patients with rocky mountain spotted fever (rmsf), a tickborne infection caused by rickettsia rickettsii, respond quickly to tetracycline-class antibiotics (e.g., doxycycline) when therapy is started within the first few days of illness; however, untreated rmsf may result in severe illness and death. persons aged <10 years have the highest age-specific incidence of rmsf. this report summarizes the clinical course and outcome of rmsf in four children from four regions of the united states and und ...	2000	11055741
effect of antipyretic therapy on the duration of illness in experimental influenza a, shigella sonnei, and rickettsia rickettsii infections.	to determine whether antipyretic therapy prolongs the course of experimental influenza a, shigella sonnei, and rickettsia rickettsii infections.	2000	11130213
ultrastructure of rickettsia rickettsii actin tails and localization of cytoskeletal proteins.	actin-based motility (abm) is a mechanism for intercellular spread that is utilized by vaccinia virus and the invasive bacteria within the genera rickettsia, listeria, and shigella. within the rickettsia, abm is confined to members of the spotted fever group (sfg), such as rickettsia rickettsii, the agent of rocky mountain spotted fever. infection by each agent induces the polymerization of host cell actin to form the typical f (filamentous)-actin comet tail. assembly of the actin tail propels t ...	2000	10899876
q fever endocarditis associated with extensive serological cross-reactivity.		2000	10722459
in vitro activities of telithromycin (hmr 3647) against rickettsia rickettsii, rickettsia conorii, rickettsia africae, rickettsia typhi, rickettsia prowazekii, coxiella burnetii, bartonella henselae, bartonella quintana, bartonella bacilliformis, and ehrlichia chaffeensis.	in vitro activities of telithromycin compared to those of erythromycin against rickettsia spp., bartonella spp., coxiella burnetii, and ehrlichia chaffeensis were determined. telithromycin was more active than erythromycin against rickettsia, bartonella, and coxiella burnetii, with mics of 0.5 microg/ml, 0.003 to 0.015 microg/ml, and 1 microg/ml, respectively, but was inactive against ehrlichia chaffeensis.	2000	10770788
rodent ectoparasites from two locations in northwestern florida.	from feb.-apr. 1999, 19 species of ectoparasitic arthropods (2 sucking lice, 4 fleas, 4 ticks, 2 mesostigmatid mites, 5 chiggers, 2 fur mites) were recovered from 106 rodents belonging to 5 species (cotton mouse, peromyscus gossypinus, n = 64; cotton rat, sigmodon hispidus, n = 23; eastern woodrat, neotoma floridana, n = 9; golden mouse, ochrotomys nuttalli, n = 9; eastern gray squirrel, sciurus carolinensis, n = 1) at tall timbers research station, leon county, florida. during the same period, ...	2000	11217221
[intraluminal diverticulum of the duodenum. report of a case and review of published cases].	the intraluminal diverticulum of the duodenum is very rare; it is an anatomical sac like formation, located in the interior of the duodenum and communicating with it through an orifice situated a the upper pole of the diverticulum where the wall is covered by mucosa on both faces. it has interesting etiopathogenic problems. we present an observation after reviewing the corresponding literature. it was a 32-year old woman suffering from moderate epigastric pains, pyrosis, chronic constipation, ma ...	2000	97140
pregnancy in a bicornuate uterus.		2000	97325
variant angina pectoris. clinical presentations, coronary angiographic patterns, and the results of medical and surgical management in 42 consecutive patients.		2000	95923
[problems of electrocardiographic and vectorcardigoraphic diagnosis in postero-basal myocardial infarct and in right branch block].		2000	97684
fluorescence polarization and pulse width analysis of chromosomes by a flow system.	isolated chinese hamster chromosomes have been analyzed using a multiparameter computer-controlled cell sorter to obtain information about unique properties of individual chromosomes. parameters other than dna content were sought that would further aid in distinguishing among chromosomes. the polarized emission of the dna-specific bis-benzimidazole dye hoechst 33342 was measured for each class of chromosomes identified by a distinct peak, i.e., differeing in dna content. the emission anisotropy ...	2000	86570
[the medical dialogue in medical education].		2000	101789
two-dimensional thin-layer chromatography for the screening of disorders of purine and pyrimidine metabolism.	a method is presented for the two-dimensional thin-layer chromatographic screening of purines, pyrimidines and their nucleosides in the urine. prior to chromatography, isolation of these substances from the urine is performed by anion-exchange column chromatography. purines and pyramidines are quantitatively eluted with formic acid 0.01 m and 4 m respectively. the results of recovery and stability experiments are given. normal excretory patterns are presented. also results in patients with vario ...	2000	95907
[neurofibromatosis in childhood].		2000	97181
pseudopapilledema.		2000	88936
sulfur-containing metabolites of chlorobiphenyl isomers, a comparative study.		2000	97335
[choice of antibiotics by young physicians].		2001	98885
hysterographic double-outlined uterine cavity: a sign of unsuspected pregnancy.	a review of three hysterograms inadvertently performed during early pregnancy reaffirms the regular appearance of infiltration of aqueous opaque media into the substance of the decidua, producing the sign of the double-outlined uterine cavity. although such endometrial opacification may also occur during the late secretory phase, its recognition should arouse suspicion of pregnancy. a coexisting intrauterine filling defect may then be more confidently diagnosed as an amniotic sac.	2001	98013
cimetidine for hypertrophic prostate?		2001	88991
sleep patterns in kleine-levin syndrome.	diurnal and nocturnal sleep records were obtained from a male and a female with kleine-levin syndrome, during excessive daytime sleep attacks and while they were asymptomatic. a common pattern of abnormal sleep was seen in both patients even during the asymptomatic period. the female, aflicted with a severe form of periodic hypersomnia, showed nocturnal and diurnal sleep onset rem periods. the different pattern of sleep abnormality in the female could be an expression of the severity of her symp ...	2001	90606
"maxillary sinusitis" and cancer: the role of polytomography.		2001	98016
catering for special needs.		2001	100095
performance of home blood-glucose meters.		2001	87914
instability of cerebral blood-flow in insulin-dependent diabetics.	cerebral blood-flow (cbf) was measured in 23 insulin-dependent diabetics before and 2--3 h after insulin/breakfast. in 10 of these patients cbf changed significantly during the day, with a significant fall in 9. in 3 patients who had the greatest falls in cbf there was a concomitant sensation of an impending faint. thus cbf is unstable in insulin-dependent diabetics in contrast to control subjects in who there is little or no variation. sudden falls in cbf in insulin-dependent diabetics may expl ...	2001	92619
child-resistant containers really are effective.		2001	90232
changing patterns in the management of small bowel fistulas.		2001	98069
food allergy and migraine.		2001	88593
[treatment of delirium tremens with haloperidol (author's transl)].		2001	98722
formaldehyde toxicity.		2001	90277
evidence of rodent-associated bartonella and rickettsia infections among intravenous drug users from central and east harlem, new york city.	we tested serum samples collected in 1997 and 1998 from a cohort of 204 injection drug users (idus) recruited from central and east harlem, new york city, new york, for antibodies reactive with seven rickettsial or bartonella spp. antigens. rodent-associated bartonella elizabethae and rickettsia akari were the primary etiologic agents of interest. the testing panel also included bartonella henselae, bartonella quintana, rickettsia prowazekii, rickettsia rickettsii, and rickettsia typhi. the high ...	2001	11791987
molecular cloning, sequence and characterization of cjst, a putative protease from rickettsia rickettsii.	the cloning and sequencing of a gene from rickettsia rickettsii which confers haemolytic activity on escherichia coli strain tb1 is described. the open reading frame of the haemolysis-promoting gene, cjst, is 1041 bp and encodes a putative protein with a molecular mass of 33 825 da. cjst has high sequence similarity to several bacterial proteases, particularly type iv signal peptidases. cell lysates from an e. coli clone containing cjst in puc19 (pjon1) exhibited greater protease activity in fun ...	2001	11312615
assessing the association between the geographic distribution of deer ticks and seropositivity rates to various tick-transmitted disease organisms in dogs.	to determine whether the geographic distribution of deer ticks (ixodes scapularis) was associated with the distribution of dogs seropositive for various tick-transmitted disease organisms (ie, borrelia burgdorferi, rickettsia rickettsii, the human granulocytic ehrlichiosis [hge] agent, ehrlichia canis, and bartonella vinsonii subsp berkhoffii).	2001	11318358
[ehrlichiosis infection prevalence in spain or cross reactions].		2001	11333754
quantitative analyses of variations in the injury of endothelial cells elicited by 11 isolates of rickettsia rickettsii.	eleven isolates of spotted fever group rickettsiae from the blood of patients or ixodid ticks from north and south america were characterized. all isolates were identified as rickettsia rickettsii using restriction fragment length polymorphism analysis of a 532-bp rompa gene fragment obtained by pcr. the ability of the r. rickettsii isolates to elicit cytopathic effects and parameters of oxidative injury were examined in cultured human ea.hy 926 endothelial cells. cytopathic effects were determi ...	2001	11427428
serological evidence for the continued presence of human rickettsioses in southern india.	rickettsiosis is generally believed to have disappeared from many parts of india. however, the serological testing of 37 residents of southern india who presented with fever of unknown aetiology in 1996-1998 confirmed that spotted fever, epidemic/endemic typhus and scrub typhus continue to occur in southern india. the epidemiology and magnitude of the problem need to be evaluated.	2001	11454249
phylogeny of rickettsia spp. inferred by comparing sequences of 'gene d', which encodes an intracytoplasmic protein.	'gene d' is the ps120-protein-encoding gene, first described in rickettsia conorii and rickettsia japonica. sequence analysis of a 3030 bp fragment of 'gene d' in 24 representatives of the genus rickettsia was carried out to complete phylogenetic analyses previously inferred by comparison of gene sequences encoding citrate synthase, 17 kda antigen and rompa and rompb. the phylogenetic relationships between rickettsiae were inferred from the comparison of both the gene and the derived protein seq ...	2001	11491333
serologic and molecular evidence of coinfection with multiple vector-borne pathogens in dogs from thailand.	forty-nine dogs from thailand were evaluated for serologic evidence of exposure or polymerase chain reaction (pcr) evidence of infection with vectorborne pathogens, including ehrlichia sp. (ehrlichia canis, ehrlichia chaffeensis, ehrlichia equi, and ehrlichia risticii), bartonella vinsonii subsp. berkhoffi (bvb), spotted fever group (sfg) rickettsiae (rickettsia rickettsii), typhus group (tg) rickettsiae (rickettsia canada, rickettsia prowazekii, and rickettsia typhi), and babesia sp. (babesia c ...	2001	11596732
spotted fever in brazil: a seroepidemiological study and description of clinical cases in an endemic area in the state of são paulo.	during 1985-1995, illnesses clinically and epidemiologically compatible with brazilian spotted fever were identified in 17 patients in the county of pedreira, in the state of são paulo, brazil. spotted-fever group rickettsial infection was confirmed by serology and/or immunostaining of tissues in 10 of these patients. immunostaining confirmed infection in a 37-year-old pregnant patient, although rickettsial antigens were not demonstrable in the tissues of the fetus. a serosurvey was conducted in ...	2001	11693878
risk of lyme disease: perceptions of residents of a lone star tick-infested community.	lone star ticks (amblyomma americanum) have been suggested as a vector of the agent of lyme disease (borrelia burgdorferi sensu lato) in the usa, based on associations with an infection manifesting mainly as erythema migrans. in laboratory experiments, however, they failed to transmit b. burgdorferi sensu stricto.	2001	11693973
urban zoonoses caused by bartonella, coxiella, ehrlichia, and rickettsia species.	the last half of the 20th century witnessed an increase in the occurrence and recognition of urban zoonoses caused by members of the genera bartonella, coxiella, ehrlichia, and rickettsia, all traditionally considered to be members of the family rickettsiaceae. in recent years, new human pathogens (bartonella elizabethae, bartonella henselae, and rickettsia felis) have been recognized in urban environments. other newly recognized pathogens (ehrlichia chaffeensis and ehrlichia phagocytophila in t ...	2001	12653141
fort chaffee revisited: the epidemiology of tick-borne rickettsial and ehrlichial diseases at a natural focus.	a retrospective cohort study was conducted among troops training at fort chaffee, arkansas, from may through june 1997, to identify infections caused by tick-borne pathogens. serum samples were tested by ifas for antibodies to selected rickettsia and ehrlichia species and by an investigational eia for spotted fever group rickettsia lipopolysaccharide antigens. of 1,067 guardsmen tested, 162 (15.2%) had antibodies to one or more pathogens. of 93 guardsmen with paired serum samples, 33 seroconvert ...	2001	12653142
canine rocky mountain spotted fever: a retrospective study of 30 cases.	rocky mountain spotted fever (rmsf) was diagnosed in 30 dogs examined at north carolina state university, veterinary teaching hospital between 1984 and 1997. historical, physical examination, and laboratory abnormalities were reviewed. diagnostic criteria included a four-fold rise in antibody titer to rickettsia rickettsii (r. rickettsii) (n=15) or a single r. rickettsii antibody titer of 1:1,024 or greater (n=15; when this initial titer was determined one week or more after the onset of clinica ...	2001	11204476
a serological survey of rural dogs and cats on the southwestern canadian prairie for zoonotic pathogens.	a survey for antibodies against agents of plague, tularemia, and rocky mountain spotted fever (rmsf), and against sin nombre hantavirus (snv), bartonella henselae and b. clarridgeiae was conducted in the summer of 1995 using serum from rural dogs and cats living in the vicinity of four public parks in southeastern alberta and southwestern saskatchewan. antibodies to all pathogens were detected in all survey areas. overall prevalence rates were 0.075 for yersinia pestis, 0.089 for francisella tul ...	2001	11257996
immunization with a portion of rickettsial outer membrane protein a stimulates protective immunity against spotted fever rickettsiosis.	two approaches for presentation of a part of the rickettsial outer membrane protein a (ompa) of rickettsia rickettsii, namely (1) recombinant mycobacterium vaccae (rmv) or (2) recombinant dna vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, r. conorii, in mice. after primary immunization with rmv and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. dna vaccinatio ...	2001	11738766
immunoperoxidase technics in diagnostic pathology. report of a workshop sponsored by the national cancer institute.		2001	88175
repeated gene families in drosophila melanogaster.		2001	98263
congenital diverticuli of the anterior urethra.		2001	98091
endothelial cell apoptosis in sepsis.	to discuss a potential role for endothelial cell apoptosis in the pathogenesis of sepsis.	2002	12004240
rickettsia rickettsii: as virulent as ever.		2002	12201574
rocky mountain spotted fever.		2002	12458609
fever and rash in a 3-year-old girl: rocky mountain spotted fever.	initial symptoms of rocky mountain spotted fever (rmsf), a tick-borne illness caused by rickettsia rickettsii, are nonspecific and include headache, gastrointestinal disturbances, malaise, and myalgias, followed by fever and rash. the classic triad of fever, rash, and history of tick exposure is uncommon at presentation. clinical manifestations of rmsf range from virtually asymptomatic to severe. because of the potentially fatal outcome of rmsf, presumptive clinical diagnosis and empiric antimic ...	2002	12353892
[emerging and reemerging rickettsiosis in an endemic area of minas gerais state, brazil].	this article describes a serological survey for rickettsiosis in the county of novo cruzeiro, minas gerais state, brazil, in 1998, testing schoolchildren and dogs. sera included 331 samples from schoolchildren from an endemic area and 142 samples from schoolchildren from a non-endemic area in the county. all children examined were healthy and had not reported clinical symptoms of brazilian spotted fever prior to the serological survey. some 35 children in the endemic area were reactive to ricket ...	2002	12488886
rocky mountain spotted fever: a review for the pediatrician.		2002	11905289
atypical fulminant rickettsia rickettsii infection (brazilian spotted fever) presenting as septic shock and adult respiratory distress syndrome.	brazilian spotted fever, caused by rickettsia rickettsii, has been increasingly reported in brazil especially in the southeastern states. the severe and fulminant forms of the disease are not unusual but most of the reported fatal cases have shown some typical clinical clue, which leads the attending physician to a correct diagnosis. we report a probable case of atypical fulminant brazilian spotted fever that presented full-blown septic shock associated with adult respiratory distress syndrome ( ...	2002	11980610
rocky mountain spotted fever.	rocky mountain spotted fever (rmsf) is a fulminant tick-borne infection by rickettsia rickettsii. the classical diagnostic triad is fever, headache and rash in a patient with a recent tick bite. with prompt, appropriate antimicrobial therapy, patient outcomes are excellent. however, rmsf is often misdiagnosed, particularly during the initial stages of illness, and as a result, patient outcomes are sometimes suboptimal.	2002	11982306
rickettsia rickettsii infection of cultured human endothelial cells induces heme oxygenase 1 expression.	existing evidence suggests that oxidative insults and antioxidant defense mechanisms play a critical role in the host cell response during infection of endothelial cells by rickettsia rickettsii, the causative agent of rocky mountain spotted fever. heme oxygenase (ho), a rate-limiting enzyme in the pathway for heme catabolism, protects against oxidant damage in a variety of stress situations. here, we report on the expression of the inducible and constitutive ho isozymes, ho-1 and ho-2, during r ...	2002	12117910
short report: concurrent rocky mountain spotted fever in a dog and its owner.	a sequential occurrence of rocky mountain spotted fever (rmsf) in a dog and its owner is described. diagnosis of rmsf in the animal guided subsequent testing for and diagnosis of the same disease in the human patient. previous reports of concurrent rmsf in dogs and their owners are reviewed, and the epidemiologic significance of this occurrence is discussed.	2002	12135293
brazilian spotted fever: description of a fatal clinical case in the state of rio de janeiro.	we describe a case of brazilian spotted fever in a previously healthy young woman who died with petechial rash associated to acute renal and respiratory insufficiency 12 days following fever, headache, myalgia, and diarrhea. serologic test in a serum sample, using an immunofluorescence assay, revealed reactive igm/igg.	2002	12621675
[shock (peripheral circulatory insufficiency). i].		2002	119989
triangulation for removal of foreign bodies: two illustrative cases.		2002	128148
a simple method for measuring the frequency response of an electromagnetic flowmeter.		2002	127898
the measurement of oestrone, equilin and dehydroepiandrosterone in the peripheral plasma of pregnant pony mares by radioimmunoassay.		2002	154595
continuing medical education resources for the practicing rhode island physician.		2002	124077
[the effectiveness of vasopressors and vasodilators for pharmaco-dynamic renal angiography (author's transl)].	9,450 radiographs of 355 patients were analysed in order to determine the diagnostic values of pharmaco-dynamic renal angiography. in each case renal angiography was performed by a conventional techinique as the basis for a clinical experimental study of the effectiveness of a-renergic and cholinergic drugs. the comparison showed unequivocally that pharmacodynamic methods may increase the value of renal angiography. vasopressors alter the pressure gradient during the arterial phase and improve f ...	2002	131081
[rehabilitation].		2002	130696
assess the workplace.		2002	3369764
an analysis of senile changes in the palpebral fissure.	this work was carried out to determine the changes in conformity and in physiological movements of the eyelids with advancing age. some conceptions fostered in the literature are false and should be discarded. others have been corroborated. we conclude that the whole lateral canthus becomes lax and drifts medially with age. therefore, surgical correction should aim at restoring the fixation of the lateral canthus to its youthful position.	2002	1122413
[dispensarization and periodic follow-up of the child with post-streptococcal r.a.a. in a group of school children].		2002	120066
histologic variants of infiltrating lobular carcinoma of the breast.	thirty-eight breasts with lobular carcinoma in situ and an additional intraductal or infiltrating cancer were studied. twenty-six of the additional cancers were diagnosed as infiltrating lobular cancer on the basis of a single population of small uniform cells cytologically identical to those in lobular carcinoma in situ. in 20 of these cases the conventional pattern of infiltrating lobular cancer was evident with an individual cell infiltrate with foci of single filing. in the other six there w ...	2003	166034
[on the 50th birthday of prof r. o. amirov].		2003	149700
[transketolase activity as a parameter for the vitamin b1 provision in some dermatoses (herpes zoster, psoriasis, drug eruptions, excema, and urticaria) (author's transl)].		2003	153861
[prevention of recurring adhesions after tuboplasty. ii. importance of grafts with free pezitoneum (author's transl)].	we have greatly improved in prevention of adhesions after tuboplasty, using peritoneal grafts on crude surfaces (28 cases off 92 tuboplasties). first results seem to confirm the interest of this method.	2003	161984
expression of inducible angiosperm promoters in a gymnosperm, picea glauca (white spruce).	electrical discharge particle acceleration was used to test the transient expression of numerous inducible angiosperm promoters in a gymnosperm picea glauca (white spruce). promoter expression was assayed in three different tissues capable of in vitro regeneration, zygotic embryos, seedlings and embryogenic callus. the promoters tested include the light-inducible arabidopsis and soybean ribulose-1,5-bisphosphate small subunit promoters and a maize phosphoenolpyruvate carboxylase promoter; a soyb ...	2003	1868222
identification of actin kinase activity in purified fragmin-actin complex.	actin kinase phosphorylates actin of fragmin-actin complex, resulting in the inactivation of the nucleation and capping activities of the complex. fragmin-actin complex was prepared by a new purification procedure. incubation with atp caused inactivation of the purified complex and phosphorylation of actin of fragmin-actin complex. the detailed analysis of the complex by sds-gel electrophoresis showed that actin kinase was co-purified with the fragmin-actin complex. formation of such an associat ...	2003	1326442
nuclear factor kappa b protects against host cell apoptosis during rickettsia rickettsii infection by inhibiting activation of apical and effector caspases and maintaining mitochondrial integrity.	apoptotic host cell death is a critical determinant in the progression of microbial infections and outcome of resultant diseases. the potentially fatal human infection caused by rickettsia rickettsii, the etiologic agent of rocky mountain spotted fever, involves the vascular endothelium of various organ systems of the host. earlier studies have shown that survival of endothelial cells (ec) during this infection depends on their ability to activate the transcription factor nuclear factor kappa b ...	2003	12819104
physician knowledge of the diagnosis and management of rocky mountain spotted fever: mississippi, 2002.	rocky mountain spotted fever (rmsf), a tick-borne illness that has its highest incidence in the south central and southeastern united states, is often a diagnostic challenge, as patients frequently present with nonspecific symptoms during the early stages of illness. rmsf has a high case fatality rate among untreated individuals, and the median time from onset of symptoms to death is only eight days, making early recognition and treatment of rmsf crucial. in two mississippi public health distric ...	2003	12860642
the immunologic response of dogs to bartonella vinsonii subspecies berkhoffii antigens: as assessed by western immunoblot analysis.	bartonella vinsonii subspecies berkhoffii is a recently recognized zoonotic pathogen that causes endocarditis, granulomatous rhinitis, and granulomatous lymphadenitis in dogs. isolation of b. vinsonii (berkhoffii) from blood or tissue samples is frequently unsuccessful; therefore, diagnosis is primarily dependent on serologic or molecular testing modalities. because previous canine serologic studies have used an indirect immunofluorescence assay (ifa), without western immunoblot (wi) confirmatio ...	2003	12918816
rickettsia rickettsii infection in the pine vole, microtus pinetorum: kinetics of infection and quantitation of antioxidant enzyme gene expression by rt-pcr.	the pine vole, microtus pinetorum, was evaluated as a laboratory animal model for infection with rickettsia rickettsii. voles demonstrated signs of acute disease, and 45% of infected animals died following intraperitoneal infection with 3 x 10(6) plaque forming units of r. rickettsii. spleen, liver, kidney, lung, brain, testes and blood were analyzed for rickettsial burden by a quantitative pcr assay. the distribution of rickettsiae in tissues during the course of infection was determined by imm ...	2003	12860675
comparison of pcr assays for detection of the agent of human granulocytic ehrlichiosis, anaplasma phagocytophilum.	human granulocytic ehrlichiosis is an emerging infectious disease in the united states and europe, and pcr methods have been shown to be effective for the diagnosis of acute infections. numerous pcr assays and primer sets have been reported in the literature. the analytical sensitivities (limits of detection) of 13 published pcr primer sets were compared using dna extracted from serial dilutions of anaplasma phagocytophilum-infected hl-60 cells. the specificity of the assays that were able to de ...	2003	12574272
deep origin of plastid/parasite atp/adp translocases.	membrane proteins that transport atp and adp have been identified in mitochondria, plastids, and obligate intracellular parasites. the mitochondrial atp/adp transporters are derived from a broad-specificity transport family of eukaryotic origin, whereas the origin of the plastid/parasite atp/adp translocase is more elusive. here we present the sequences of five genes coding for atp/adp translocases from four species of rickettsia. the results are consistent with an early duplication and divergen ...	2003	12574860
effects of ectopically expressed neuronal wiskott-aldrich syndrome protein domains on rickettsia rickettsii actin-based motility.	neuronal wiskott-aldrich syndrome protein (n-wasp) and the actin-related protein 2/3 (arp2/3) complex have emerged as critical host proteins that regulate pathogen actin-based motility. actin tail formation and motility in listeria monocytogenes require the arp2/3 complex but bypasses n-wasp signaling. motility of shigella flexneri and vaccinia virus requires both n-wasp and the arp2/3 complex. functional roles for these cytoskeletal regulatory proteins in actin-based motility of rickettsia rick ...	2003	12595475
phylogenetic analysis of the erythrocytic anaplasma species based on 16s rdna and groel (hsp60) sequences of a. marginale, a. centrale, and a. ovis and the specific detection of a. centrale vaccine strain.	phenotypic criteria for the identification of erythrocytic ruminant anaplasma species has relied on subjective identification methods such as host pathogenicity (virulence for cattle or sheep) and/or the location of anaplasma inclusion bodies within the host's red cells. sequence comparisons of new and available genbank accessions were investigated to elucidate the relationships among these closely related anaplasma species. twenty-one 16s rdna and groel (hsp60) sequences from 13 anaplasma margi ...	2003	12488078
antibodies reactive to rickettsia rickettsii among children living in the southeast and south central regions of the united states.	the reported annual incidence of rocky mountain spotted fever in the united states is 2.2 per million, but studies have suggested that human infection with rickettsia rickettsii may be more common. this study estimated the prevalence of antibodies reactive to r rickettsii among children living in the southeastern and south central united states.	2003	12742879
characterization of the ftsz gene from ehrlichia chaffeensis, anaplasma phagocytophilum, and rickettsia rickettsii, and use as a differential pcr target.	degenerate primers corresponding to highly conserved regions of previously characterized ftsz genes were used to pcr amplify a portion of the ftsz gene from the genomic dna of ehrlichia chaffeensis (ftsz(ech)), anaplasma phagocytophilum (ftsz(ap)), and rickettsia rickettsii (ftsz(rr)). genome walking was then used to amplify the 5' and 3' termini of the genes. the dna sequences of the resulting amplification products yielded open reading frames coding for proteins with molecular masses of 42.0, ...	2003	12804116
rickettsial actin-based motility: behavior and involvement of cytoskeletal regulators.	actin-based motility (abm) is employed by spotted fever group (sfg) rickettsiae, such as rickettsia rickettsii, to promote cell-to-cell spread. time-lapse video microscopy revealed that abm is not strictly confined to sfg rickettsiae as typhus group r. typhi moved at approximately the same rate as r. rickettsii (approximately 4 micro m/min), but in a highly erratic fashion. a number of common behaviors were observed between abm of r. typhi and r. rickettsii, such as entrance into plasma membrane ...	2003	12860687
interactions of rickettsia rickettsii with endothelial nuclear factor-kappab in a "cell-free" system.		2003	12860701