Publications

defining potential vaccine targets of haemophilus ducreyi trimeric autotransporter adhesin dsra.	haemophilus ducreyi is the causative agent of the sexually transmitted genital ulcer disease chancroid. strains of h. ducreyi are grouped in two classes (i and ii) based on genotypic and phenotypic differences, including those found in dsra, an outer membrane protein belonging to the family of multifunctional trimeric autotransporter adhesins. dsra is a key serum resistance factor of h. ducreyi that prevents binding of natural igm at the bacterial surface and functions as an adhesin to fibronect ...	2015	25897604
complete genome sequences of 11 haemophilus ducreyi isolates from children with cutaneous lesions in vanuatu and ghana.	haemophilus ducreyi causes chancroid and has recently been shown to be a significant cause of cutaneous lesions in tropical or subtropical regions where yaws is endemic. here, we report the draft genome assemblies for 11 cutaneous strains of haemophilus ducreyi, isolated from children in vanuatu and ghana.	2016	27389258
host polymorphisms in tlr9 and il10 are associated with the outcomes of experimental haemophilus ducreyi infection in human volunteers.	in humans inoculated with haemophilus ducreyi, there are host effects on the possible clinical outcomes-pustule formation versus spontaneous resolution of infection. however, the immunogenetic factors that influence these outcomes are unknown. here we examined the role of 14 single-nucleotide polymorphisms (snps) in 7 selected pathogen-recognition pathways and cytokine genes on the gradated outcomes of experimental infection.	2016	27122592
draft whole-genome sequence of haemophilus ducreyi strain auspng1, isolated from a cutaneous ulcer of a child from papua new guinea.	haemophilus ducreyi has recently emerged as a leading cause of cutaneous ulcers in the yaws-endemic areas of papua new guinea and other south pacific islands. here, we report the draft genome sequence of the h. ducreyi strain auspng1, isolated from a cutaneous ulcer of a child from papua new guinea.	2016	26847887
immunization with the haemophilus ducreyi trimeric autotransporter adhesin dsra with alum, cpg or imiquimod generates a persistent humoral immune response that recognizes the bacterial surface.	the ducreyi serum resistance a (dsra) protein of haemophilus ducreyi belongs to a large family of multifunctional outer membrane proteins termed trimeric autotransporter adhesins responsible for resistance to the bactericidal activity of human complement (serum resistance), agglutination and adhesion. the ability of dsra to confer serum resistance and bind extracellular matrix proteins lies in its n-terminal passenger domain. we have previously reported that immunization with a recombinant form ...	2016	26812077
haemophilus ducreyi: from sexually transmitted infection to skin ulcer pathogen.	this article provides an overview of the biology, epidemiology, clinical features, diagnostic tests, and treatment of haemophilus ducreyi infection, with special reference to the decline of chancroid and the recent emergence of h. ducreyi as a pathogen responsible for chronic limb ulceration clinically similar to yaws.	2016	26658654
first case of chancroid in 14 years at the largest sti clinic in paris, france.	we report the first case of chancroid seen at our clinic in 14 years. it was diagnosed by nuclear acid amplification test in a male patient returning from madagascar. although the disease is considered on the verge of disappearance even in tropical countries, its real potential for reemergence - due to new strains of haemophilus ducreyi, underreporting and a lack of widespread use of molecular testing - could be underestimated.	2016	26378189
tropical leg ulcers in children: more than yaws.	the management of yaws has changed in recent years. mass treatment with oral azithromycin has replaced intramuscular benzathine benzylpenicillin. treponemal and non-treponemal serology (equivalent to tpha and rpr) point-of-care blood testing is now available. in addition, recent studies in yaws endemic regions have shown that a significant number of leg ulcers in children which are clinically suggestive of yaws are caused by haemophilus ducreyi. it is noteworthy that the world health organizatio ...	2016	26289420
impact of cdt toxin on human diseases.	cytolethal distending toxin (cdt) is found in gram-negative bacteria, especially in certain proteobacteria such as the pasteurellaceae family, including haemophilus ducreyi and aggregatibacter (actinobacillus) actinomycetemcomitans, in the enterobacteriaceae family and the campylobacterales order, including the campylobacter and helicobacter species. in vitro and in vivo studies have clearly shown that this toxin has a strong effect on cellular physiology (inflammation, immune response modulatio ...	2016	27429000
haemophilus ducreyi seeks alternative carbon sources and adapts to nutrient stress and anaerobiosis during experimental infection of human volunteers.	haemophilus ducreyi causes the sexually transmitted disease chancroid in adults and cutaneous ulcers in children. in humans, h. ducreyi resides in an abscess and must adapt to a variety of stresses. previous studies (d. gangaiah, m. labandeira-rey, x. zhang, k. r. fortney, s. ellinger, b. zwickl, b. baker, y. liu, d. m. janowicz, b. p. katz, c. a. brautigam, r. s. munson, jr., e. j. hansen, and s. m. spinola, mbio 5:e01081-13, 2014, http://dx.doi.org/10.1128/mbio.01081-13) suggested that h. ducr ...	2016	26930707
correlates of bacterial ulcers and acute hsv-2 infection among men with genital ulcer disease in south africa: age, recent sexual behaviors, and hiv.	data from baseline surveys and sti/hiv laboratory tests (n=615 men) were used to examine correlates of bacterial ulcers (treponema pallidum, haemophilus ducreyi, or chlamydia trachomatis l1-l3 detected in ulcer) and acute hsv-2 ulcers (hsv-2 positive ulcer specimen, hsv-2 sero-negative, and negative for bacterial pathogens) vs. recurrent hsv-2 ulcers (sero-positive), separately. compared to men with recurrent hsv-2 ulcers, men with bacterial ulcers had larger ulcers but were less likely to be hi ...	2016	28217702
etiology of genital ulcer disease in male patients attending a sexually transmitted diseases clinic: first assessment in cuba.	sexually transmitted diseases (stds) and in particular genital ulcer disease (gud) have a major impact on morbidity and mortality in developing countries. the world health organization recommends the use of syndromic guidelines for the treatment of sexually transmitted infections (stis) in resource-constrained countries. surveillance of autochthonous etiologies provides epidemiological information contributing to the prevention and treatment of stis. we investigated the etiology and factors asso ...	2016	27419816
multiplex pcr testing for nine different sexually transmitted infections.	current sexually transmitted infection (sti) testing is not optimal due to delays in reporting or missed diagnoses due to a lack of comprehensive testing. the filmarray® (biofire diagnostics, llc, salt lake city, utah) is a user-friendly, fully automated, multiplex pcr system that is being developed for rapid point-of-care use. a research-use-only sti panel including multiple pcr primer sets for each organism was designed to detect chlamydia trachomatis, neisseria gonorrhoeae, treponema pallidum ...	2016	26538551
sexually transmitted diseases: epidemiological and clinical aspects in adults.	sexually transmitted diseases (stds) are the first 10 causes of unpleased diseases in young adult women in the world. the concept of stds includes a series of syndromes caused by pathogens that can be acquired by sexual intercourse or sexual activity.adolescents and young adults are responsible for only 25% of the sexually active population and they represent almost 50% of all newly acquired stds.in this way, we evaluated the epidemiological and clinical aspects of most relevant pathogens as nei ...	2016	25532465
haemophilus ducreyi cutaneous ulcer strains diverged from both class i and class ii genital ulcer strains: implications for epidemiological studies.	haemophilus ducreyi has emerged as a major cause of cutaneous ulcers (cu) in yaws-endemic regions of the tropics in the south pacific, south east asia and africa. h. ducreyi was once thought only to cause the genital ulcer (gu) disease chancroid; gu strains belong to 2 distinct classes, class i and class ii. using whole-genome sequencing of 4 cu strains from samoa, 1 from vanuatu and 1 from papua new guinea, we showed that cu strains diverged from the class i strain 35000hp and that one cu strai ...	2016	28027326
yaws: towards the who eradication target.	in 2012 who declared a target to eradicate yaws by 2020. the cornerstone of this strategy is community mass treatment with azithromycin. initial studies suggest this is a very effective tool that may be capable of interrupting transmission. alongside this there has been progress in the development and validation of diagnostic tests for yaws. several new challenges have also emerged, in particular, evidence that haemophilus ducreyi can cause phenotypically similar ulcers in yaws endemic communiti ...	2016	27268712
epidemiology of haemophilus ducreyi infections.	the global epidemiology of haemophilus ducreyi infections is poorly documented because of difficulties in confirming microbiological diagnoses. we evaluated published data on the proportion of genital and nongenital skin ulcers caused by h. ducreyi before and after introduction of syndromic management for genital ulcer disease (gud). before 2000, the proportion of gud caused by h. ducreyi ranged from 0.0% to 69.0% (35 studies in 25 countries). after 2000, the proportion ranged from 0.0% to 15.0% ...	2016	26694983
2017 european guideline for the management of chancroid.	chancroid is a sexually acquired infection caused by haemophilus ducreyi. the infection is characterized by one or more genital ulcers, which are soft and painful, and regional lymphadenitis, which may develop into buboes. the infection may easily be misidentified due to its rare occurrence in europe and difficulties in detecting the causative pathogen. h. ducreyi is difficult to culture. nucleic acid amplification tests can demonstrate the bacterium in suspected cases. antibiotics are usually e ...	2017	28081686
a novel intrinsically disordered outer membrane lipoprotein of aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8.	intrinsically disordered proteins (idps) do not have a well-defined and stable 3-dimensional fold. some idps can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. a novel outer membrane lipoprotein, bacterial interleukin receptor i (bilri) of the opportunistic oral pathogen aggregatibacter actinomycetemcomitans binds a key gatekeeper proinflammatory cytokine interleukin (il)-1β. because the amino aci ...	2017	27459270
haemophilus ducreyi in asymptomatic prostitutes.		2017	28223612
haemophilus ducreyi in asympmatic prostitutes.		2017	28223611