Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| redirection of prostaglandin endoperoxide metabolism at the platelet-vascular interface in man. | prostacyclin (pgi2) is an inhibitor of platelet function in vitro. we tested the hypothesis that pgi2 is formed in biologically active concentrations at the platelet-vascular interface in man and can be pharmacologically modulated to enhance its inhibitory properties. this became feasible when we developed a microquantitative technique that permits the measurement of eicosanoids in successive 40-microliters aliquots of whole blood emerging from a bleeding time wound. in 13 healthy volunteers the ... | 1989 | 2643628 |
| internalization and degradation of insulin by a human insulin receptor-v-ros hybrid in chinese hamster ovary cells. | chinese hamster ovary cell lines expressing either the wild-type human insulin receptor or a hybrid molecule in which the tyrosine kinase domain of the insulin receptor is replaced with that of the oncogene, v-ros were examined for their ability to internalize and degrade insulin. cells expressing the hybrid receptor were found to internalize and degrade insulin at approximately half the rate of cells expressing the native insulin receptor. moreover, insulin was incapable of inducing the interna ... | 1989 | 2645866 |
| therapeutic implications of tumor heterogeneity. | at the outset of this review, we stated that we wished to raise some questions that challenge the commonly held view that tumor heterogeneity is of major significance to treatment failure. the nature of this challenge is the following: although tumor heterogeneity in sensitivity to therapeutic agents has been demonstrated repeatedly, using isolated subpopulations of cells, primarily in cell culture systems, there is very little work that has been directed toward asking the tough questions about ... | 1989 | 2652316 |
| detection of vibrio vulnificus cytolysin in v. vulnificus-infected mice. | enzyme-linked immunosorbent assays using polyclonal and monoclonal antibodies specific for v. vulnificus cytolysin detected the toxin in an extract of skin lesions and in serum from mice showing local and systemic v. vulnificus disease after subcutaneous injection of the bacterium. the cytolysin also was detected in skin lesions by an indirect immunofluorescence procedure using polyclonal and monoclonal antibodies. our findings provide direct evidence that the cytolysin is produced in vivo durin ... | 1989 | 2658204 |
| pulsed-field gel electrophoresis of circular dna. | mobility of supercoiled (form i) and nicked circular (form ii) plasmid dnas was determined on two major forms of pulsed-field electrophoresis, chef and ofage. plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with saccharomyces cerevisiae chromosomes as standards. agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels resolving forms i and ii of smaller plasmids. the pulsing range of 3.7 to 240 seconds resulted in quite v ... | 1989 | 2662139 |
| review of cardiovascular findings in humans treated with minoxidil. | minoxidil is an antihypertensive vasodilator with no known capability of inducing human cardiovascular pathology; however, the compound does cause a late, canine-specific lesion of the right atrium and acute right atrial and ventricular papillary muscle changes. in all the autopsy cases of minoxidil-treated humans to date, 9 cases were reported with significant right atrial or other cardiac pathology and were carefully reviewed for similarity to either the early or late canine lesion. three new ... | 1989 | 2665032 |
| vibrio vulnificus infection in hawaii. | a life-threatening vibrio vulnificus infection occurred in a 52-year-old korean woman with hepatic cirrhosis. four days after ingesting raw crab, the patient presented to the hospital with nausea, vomiting, fever, hypotension, and hemorrhagic blistering of the left foot. vibrio vulnificus was recovered from both her blood and a foot wound. | 1989 | 2666323 |
| infectious and toxic syndromes from fish and shellfish consumption. a review. | primary care physicians care for large numbers of patients presenting with "food poisoning" or gastroenteritis. when a patient who presents with acute gastrointestinal illness, especially in conjunction with neurologic or cutaneous symptoms, is evaluated, the history should focus on past seafood consumption (particularly raw or undercooked seafood). the infectious syndromes are generally self-limited and respond to supportive care; exceptions are those caused by vibrio cholerae and vibrio vulnif ... | 1989 | 2669661 |
| a new procedure for the targeted inactivation of essential bacterial genes. | a new method is described for the exchange of a plasmid encoded mutant gene with its chromosomal counterpart. the method is based on positive selection and is applicable for the exchange of essential genes. the main features of the method are: (1) cloning of an antibiotic resistance marker (without its promoter) downstream of the cloned target gene, thus forming an artificial operon; (2) inactivating the target gene (and consequently also the antibiotic resistance gene) by inserting a strong tra ... | 1989 | 2674660 |
| a new procedure for the targeted inactivation of essential bacterial genes. | a new method is described for the exchange of a plasmid encoded mutant gene with its chromosomal counterpart. the method is based on positive selection and is applicable for the exchange of essential genes. the main features of the method are: (1) cloning of an antibiotic resistance marker (without its promoter) downstream of the cloned target gene, thus forming an artificial operon; (2) inactivating the target gene (and consequently also the antibiotic resistance gene) by inserting a strong tra ... | 1989 | 2674660 |
| a biotin-streptavidin amplified enzymeimmunoassay for 13,14-dihydro-15-keto-pgf2 alpha. | as an alternative for radioimmunoassays a new enzyme immunoassay (eia) for the determination of 13,14-dihydro-15-keto pgf2 alpha (pgfm) has been developed. biocytin was linked to pgfm by the n-hydroxysuccinimide method and the product (biocytinyl-pgfm) purified by reversed phase column chromatography. biocytinyl-pgfm was used in the eia as a bridge between the immobilized pgfm-antibody and streptavidin-peroxidase. the absolute sensitivity of the assay was about 160 amol (92% rel. binding) and re ... | 1989 | 2675197 |
| establishment and characterization of human glioblastoma cell lines in vitro and their xenografts in nude mice. | human gliomas were grown as permanent tissue cultures and xenografts in nude mice. growth kinetics, immunohistology and karyotypes were established. one tumor showed a distinct change in its karyotype after only two passages in nude mice; by contrast, no change of immunophenotype occurred during and after in vivo passaging. treatment of this glioma as xenograft in nude mice revealed a high sensitivity towards single agent treatment with bcnu. in view of the cytologic aberrations, however, this r ... | 1989 | 2677876 |
| digit-muscle responses evoked from multiple intracortical foci in monkey precentral motor cortex. | 1. the precentral motor cortex, including the anterior bank of the central sulcus of monkey (macaca fuscata), was systemically penetrated with microelectrodes to determine the spatial organization of the microexcitable cortical elements that can produce responses in digit muscles. 2. at 200-microns intervals on each electrode track, low-current intracortical microstimuli were delivered and the muscle responses evoked from four digit muscles were recorded. the responses, obtained with 5, 8, 15, a ... | 1989 | 2681562 |
| acute gluteal abscesses: injectable chloroquine as a cause. | one hundred and seven patients admitted to the casualty department with acute gluteal abscesses and surgically drained were studied. in 91 patients (85%) the abscess was a result of an intramuscular injection, of whom 71 patients (66%) had the abscess following a chloroquine injection (antimalarial). of those patients with an abscess following an intramuscular injection, in 64 patients (70%) a disposable syringe was used for injection of the drug and in 18% a boiled disposable was used. the inje ... | 1989 | 2681814 |
| phosphoroselenoate oligodeoxynucleotides: synthesis, physico-chemical characterization, anti-sense inhibitory properties and anti-hiv activity. | oligodeoxynucleotides with a phosphorus atom in which one of the non-bridging oxygen atoms is substituted by selenium were prepared and investigated with respect to their antisense properties. a general synthesis of phosphoroselenoate analogs of oligonucleotides is described using potassium selenocyanate as the selenium donor. the compounds, characterized by 31p nmr, were shown to decompose to phosphate with a half-life of ca. 30 days. melting temperatures of duplexes between poly(ra) or poly(ri ... | 1989 | 2682524 |
| the common src homology region 2 domain of cytoplasmic signaling proteins is a positive effector of v-fps tyrosine kinase function. | a conserved noncatalytic domain sh2 (for src homology region 2) is located immediately n terminal to the kinase domains of all cytoplasmic protein-tyrosine kinases. we found that the wild-type v-fps sh2 domain stimulated the enzymatic activity of the adjacent kinase domain 10-fold and functioned as a powerful positive effector of catalytic and transforming activities within the v-fps oncoprotein (p130gag-fps). partial proteolysis of p130gag-fps and supporting genetic data indicated that the v-fp ... | 1989 | 2685548 |
| the common src homology region 2 domain of cytoplasmic signaling proteins is a positive effector of v-fps tyrosine kinase function. | a conserved noncatalytic domain sh2 (for src homology region 2) is located immediately n terminal to the kinase domains of all cytoplasmic protein-tyrosine kinases. we found that the wild-type v-fps sh2 domain stimulated the enzymatic activity of the adjacent kinase domain 10-fold and functioned as a powerful positive effector of catalytic and transforming activities within the v-fps oncoprotein (p130gag-fps). partial proteolysis of p130gag-fps and supporting genetic data indicated that the v-fp ... | 1989 | 2685548 |
| [principle, method, and application for the production of peptide hormones using genetic engineering]. | 1989 | 2691727 | |
| falciparum malaria--present day problems. an experience with 425 cases. | clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ... | 1989 | 2693436 |
| vibrio vulnificus infection. | we report 3 cases of vibrio vulnificus infections from taiwan. patient 1, who manifested symptoms of primary septicemia, died after 2 days. patient 2, who had a wound infection and signs and symptoms of sepsis but negative blood cultures, responded to tobramycin and chloramphenicol plus surgical debridement, and recovered after 26 days of hospitalization. patient 3 had secondary septicemia originating from a wound inflicted by a shrimp. originally, the patient seemed to respond to ceftazidime an ... | 1989 | 2694352 |
| vibrio vulnificus septicaemia presenting as spontaneous necrotising cellulitis in a woman with hepatic cirrhosis. | vibrio vulnificus is a virulent marine organism commonly found in hong kong coastal waters which contaminates local sea-food. it may produce a primary septicaemia, often associated with secondary skin lesions, following ingestion of raw shell fish. we report a rapidly fatal case of primary v. vulnificus septicaemia in a 50-year-old housewife with post-hepatitic cirrhosis presenting as spontaneous necrotising cellulitis of the legs. v. vulnificus infection should be considered in patients with a ... | 1989 | 2694353 |
| parallels between neurasthenia and premenstrual syndrome. | neurasthenia and premenstrual syndrome became medical diseases because of the historical recognition of menstruation as a medical disease. both the nineteenth and twentieth century cultural views of women were important in the establishment of menstruation, neurasthenia and premenstrual syndrome as medical conditions. uncertainty of diagnosis with ever expanding diagnostic criteria, therapy undertaken without an adequate physiological basis, and often adverse effects from therapy, were character ... | 1989 | 2694626 |
| mechanism of lhrh-stimulated steroidogenesis in rat leydig cells: lipoxygenase products of arachidonic acid may not be involved. | luteinizing hormone releasing hormone agonist, [(imbzl)-dhis6,pro9,net]-lhrh (lhrh-a), caused a two to threefold increase in in vitro testosterone (t) secretion by rat leydig cells. this lhrh-a-induced t secretion was completely blocked by quinacrine and chloroquine, inhibitors of phospholipase a2. addition of phospholipase a2, however, was ineffective in stimulating basal or lhrh-a-induced t secretion. phospholipase c, on the other hand, significantly stimulated both basal and lhrh-a-induced t ... | 1989 | 2695506 |
| membrane fatty acid and virulence changes in the viable but nonculturable state of vibrio vulnificus. | the nonculturable state of vibrio vulnificus and, for comparison, that of escherichia coli were studied in artificial-seawater microcosms at 5 degrees c. total cell counts were monitored by acridine orange epifluorescence, metabolic activity by direct viable counts, and culturability by plate counts on selective and nonselective media. whereas total counts remained constant, plate counts of v. vulnificus suggested nonculturability by day 24. in contrast, direct viable counts indicated significan ... | 1989 | 2696428 |
| vibrio vulnificus sepsis after eating raw oysters. | vibrio vulnificus, a marine vibrio found in coastal waters of the united states, may contaminate certain seafoods, particularly raw oysters. patients with underlying liver disease are particularly susceptible to severe illness. unexplained febrile diseases in patients who have eaten raw oysters may be caused by vibrio vulnificus. a fatal and a nonfatal case are reported in two such patients. these patients are the first two reported cases of vibrio vulnificus infection in kentucky. | 1989 | 2723530 |
| characterization and purification of h1tf2, a novel ccaat-binding protein that interacts with a histone h1 subtype-specific consensus element. | definition of mechanisms regulating human histone h1 gene transcription during the cell cycle requires the isolation and biochemical characterization of protein factors which interact with specific promoter elements. two distinct binding activities have been identified in nuclear extracts from hela cells and mapped within a 180-base-pair (bp) region of a cell cycle-regulated h1 gene promoter. h1tf1 bound to an h1-specific a + c-rich sequence (ac box), 100 bp upstream of the cap site; h1tf2 inter ... | 1989 | 2725515 |
| primary structures of sm31/32 diagnostic proteins of schistosoma mansoni and their identification as proteases. | we have constructed cdna clones containing the complete nucleotide sequences coding for two highly antigenic schistosoma mansoni adult worm proteins, sm31 and sm32. the predicted amino acid sequence of sm31 shows significant homology to mouse, rat and human cathepsin b. the nucleotide sequence of sm32 is identical to that reported by others for s. mansoni "haemoglobinase'. the different nucleotide sequences demonstrate the existence of two different proteolytic enzymes, both of which are synthes ... | 1989 | 2725581 |
| vibrio sepsis in a cirrhotic patient. | vibrio vulnificus should be suspected in any soft tissue infection occurring after exposure to the marine environment. early recognition followed by appropriate antibiotic therapy and debridement of involved tissue are essential features in the treatment of these infections. | 1989 | 2734646 |
| ventricular arrhythmias and local electrograms after chronic regional denervation of the ischemic area in the pig heart. | cardiac denervation has been proved to reduce the incidence of coronary occlusion arrhythmias in digs, but the effect of limiting the extent of sympathectomy to the ischemic area, particularly in hearts with sparse coronary collateral circulation, as in the human heart, needs further investigation. ventricular arrhythmias and changes in epicardial direct current electrograms induced during acute left anterior descending coronary artery occlusion were recorded in 14 pigs subjected to regional den ... | 1989 | 2738265 |
| [complexing of bovine serum albumin with phosphatidyl choline liposomes]. | thermodynamic parameters of the bovine serum albumin interaction with phosphatidyl choline liposomes have been determined. it is shown that the bovine serum albumin is adsorbed on the liposomal surface without change of the globule conformation, the polar interactions making dominant contribution in immobilization. the complexing is characterized by a binding constant and a great number of binding sites. | 1989 | 2749908 |
| attempted passive transfer of a serum factor in chronic urticaria from human to monkey. | 1989 | 2757947 | |
| 2-bromo- and 16-bromo-estrogens and related compounds: potential inhibitors for both estradiol 2- and 16 alpha-hydroxylases in rat liver microsomes. | kinetic studies of inhibition of estradiol 2- and 16 alpha-hydroxylase activities in male rat liver microsomes with 2-bromoestrogens, 4-bromo-estrone (4-bre1), 16 alpha- and 16 beta-bromoestrones and 16 beta-acetylthioestrone (16-acse1) were carried out. 2-bromoestrogens and 4-bre1 nonspecifically blocked the two enzyme activities in a competitive manner, and 2-bromo-estradiol (2-bre2) was the most potent inhibitor for the two hydroxylases among the 2- and 4-bromo steroids. kinetic data, the app ... | 1989 | 2761259 |
| transcriptional start site in the mouse cyp1a1 (cytochrome p1450) gene. | two transcriptional initiation sites have been described by different laboratories for the mouse cyp1a1 (cytochrome p1450) gene: start site ti, at the beginning of the nontranslated 87-bp first exon, and start site tii, 635 bp downstream from site ti within the 2,380-bp first intron. site ti was characterized in normal c57bl/6 mouse liver, whereas site tii was described in a high activity variant line (hav cells) derived from the mouse hepatoma hepa-1c1c7 wild type (wt) established cell line. it ... | 1989 | 2766934 |
| [cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control]. | fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ... | 1989 | 2772364 |
| phagocytosis by guinea pig polymorphonuclear leukocytes of liposomes stabilized with polysaccharides. | hemolysate-loaded liposomes were prepared with their surface coated by polysaccharides, carboxymethylchitin, carboxymethylcellulose and carboxymethylchitosan. phagocytosis by guinea pig polymorphonuclear leukocytes of these coated liposomes was measured in view of electrostatic interactions between the cells and the liposomes. this view revealed to hold at least in quite an early stage of phagocytosis, but some specific interactions were assumed to exist between the cells and the liposomes. n-ac ... | 1989 | 2775873 |
| suppression of antibody responses to the acetylcholine receptor by natural antibodies. | some relationships between "natural" physiological autoreactivity and antibody responses against the acetylcholine receptor (achr) were investigated. collections of unselected monoclonal antibodies were derived from nonmanipulated newborn mice of susceptible (balb.b) and resistant (balb/c) mouse strains, and screened for reactivity with heterologous achr. five in 200 balb/c but none in 200 balb.b clones were found reactive. four of these anti-achr antibodies were analyzed for connectivity to nin ... | 1989 | 2776827 |
| virulence of vibrio vulnificus strains isolated from seafoods. | a study was carried out on the relation between virulence of v. vulnificus assessed by ld50 in mice and other markers. v. vulnificus strains isolated from seafoods had ld50 ranging from 10(3)-10(7) for mice. strains showing ld50 10(6) or less were generally haemolytic and settled after boiling. resistance to bactericidal action of serum and ability to take up congo red was shown by all the strains. | 1989 | 2777343 |
| human eosinophils express cd4 protein and bind human immunodeficiency virus 1 gp120. | the cd4 glycoprotein, expressed on leukocytes belonging to subsets of t lymphocytes and to cells of monocyte/macrophage lineage, participates in the functioning of t cells and serves as a receptor for hiv-1 and hiv-2. human eosinophils, a class of granulocytic leukocytes, have been found to express cd4. with anti-cd4 mabs cd4 was demonstrable on eosinophils from both normal and eosinophilic donors. eosinophils synthesized a 55-kd cd4 polypeptide immunoprecipitable with two anti-cd4 mabs. eosinop ... | 1989 | 2783333 |
| effect of vitamin d metabolites on calcitriol metabolism in experimental renal failure. | previous studies from our laboratory have demonstrated that metabolic clearance rate (mcr) of calcitriol is decreased in experimental renal failure. in this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin d3 (25(oh)d3) and 24,25-dihydroxyvitamin d3 (24,25(oh)2d3) on the mcr of calcitriol in renal failure produced in rats by partial nephrectomy. the mcr of calcitriol in these rats with renal failure was significantly lower than in control rats with sham operations. plasma con ... | 1989 | 2789310 |
| [intestinal microbial ecology and its modulation under the influence of immunodepressants]. | oral administration of immunodepressants such as imuran (purine analog) and batriden (gossypol derivative) for 3 months led to development of dysbacterioses in various sections of the rat gastrointestinal tract. the dysbacterioses differed in their levels and the pattern of the recovery process. as compared to batriden, imuran in a dose of 30 mg/kg body weight administered at the early observation periods (days 7, 14 and 30) induced more marked disorders in the intestine microecology. the imuran ... | 1989 | 2802878 |
| role of carboxylesterase in soman, sarin and tabun poisoning in rats. | tocp (triorthocresyl phosphate) inhibits carboxylesterase (carbe) activity in rat plasma and liver and significantly increases soman, sarin and tabun toxicity. application of these agents after tocp caused strong additional inhibition of carbe and cholinesterases (che) in rat red blood cells, plasma, liver, brain, diaphragm and intercostal muscle. after phenobarbital pretreatment, which induced carbe activity in plasma and liver by 80% and that of plasma che by 33%, acute toxicity of soman and t ... | 1989 | 2813290 |
| the dermatopathologist and human immunodeficiency virus infection. | 1989 | 2817921 | |
| an experimental study of susceptibility to infection after hemorrhagic shock. | hemorrhagic shock has been associated with an increased risk of infection after injury. the immediate and long term effects of hemorrhagic shock without tissue injury on the susceptibility of an animal to infection and the efficacy of antibiotic prophylaxis to prevent infection in this setting were examined. sprague-dawley rats were subjected to hemorrhagic shock (ld15) by bleeding to a mean arterial pressure of 45 millimeters of mercury for 45 minutes and were resuscitated with shed blood and n ... | 1989 | 2911791 |
| human milk feeding enhances the urinary excretion of immunologic factors in low birth weight infants. | the effects of fortified human milk feedings on the urinary excretion of lactoferrin, lysozyme, secretory component, iga, and secretory iga antibodies to escherichia coli o antigens were investigated in very low birth wt infants. infants were maintained on either a human milk or a cow's milk preparation. the amounts of each immune factor that were ingested and excreted were quantified during balance studies conducted at 2.5 and 5 wk of age. serum levels of these immune factors were similar in bo ... | 1989 | 2919134 |
| degradation of type ix collagen by matrix metalloproteinase 3 (stromelysin) from human rheumatoid synovial cells. | the degradation of type ix collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (mmps) purified from the culture medium of rheumatoid synovial cells. neither mmp-1 (collagenase) nor mmp-2 (so-called 'gelatinase') could digest type ix collagen, but mmp-3 (stromelysin) readily degraded it into smaller fragments. this suggests that mmp-3 may be responsible for the pathological degradation and/or normal turnover of type ix collage ... | 1989 | 2920840 |
| vibrio vulnificus corneal ulcer. case reports. | 1989 | 2923553 | |
| basic training and the part i examination. | 1989 | 267474 | |
| the life-after-death phenomenon. | the life-after-death phenomenon has created student interest and is often a topic initiated by students by students in death education courses. the article summarizes some popular publications about the life-after-death phenomenon and extracts commonalities that potentially affect other topic areas in a death education course or unit. topic areas such as coping with death, suicide and stages of dying are then examined to note the effect the phenomenon can have on the topical discussions. the art ... | 1990 | 258721 |
| structure-activity relationship of yohimbine and its related analogs in blocking alpha-1 and alpha-2 adrenoceptors: a comparative study of cardiovascular activities. | we investigated the selectivities and structure requirements for alpha-1 and alpha-2 adrenoceptor blocking activities of yohimbine (yo) and its 12 related analogs, such as beta-yohimbine (beta-yo), dihydrocorynantheine (dhc) and (-)indoloquinolizidine ((-)iq). the affinity of yo analogs to alpha-adrenoceptor was assessed by measuring their blockade of pressor responses to epinephrine in pithed rats. among yo structure groups, the potency order was yo greater than dhc = beta-yo greater than geiss ... | 1990 | 1976443 |
| cytolytic action of vibrio vulnificus haemolysin on mast cells from rat peritoneal cavity. | the mode of action of vibrio vulnificus haemolysin (vvh) on mast cells from the peritoneal cavity of the rat was examined. vvh induced histamine release, and damage to the mast cells, in a dose-dependent fashion. when 1 microgram of vvh was added to c. 10(5) mast cells at 37 degrees c, histamine release was observed after a lag period of 5-10 s, and was complete within 5 min. the action was temperature-dependent, and was not induced at 4 degrees c. disodium cromoglycate, a membrane stabiliser fo ... | 1990 | 1692881 |
| studies on the lipopolysaccharide of a virulent and an avirulent strain of vibrio vulnificus. | vibrio vulnificus is a marine bacterium associated with both primary septicemias and wound infections in humans. the lipopolysaccharides of a virulent and an avirulent strain of vibrio vulnificus were compared with respect to their chemical constituents and electrophoretic characteristics. 2-keto-3-deoxyoctonic acid, a normal constituent of the lipopolysaccharide of typical enterobacteriaceae, was not found in the lipopolysaccharide of either strain. hexadecenoate (c16:1) was the predominant fat ... | 1990 | 1693085 |
| developmental cycle-specific host-free rna synthesis in chlamydia spp. | the incorporation of radiolabeled gtp into rna in host-free chlamydia trachomatis serovar l2 organisms was investigated. the incorporation was partially inhibited by rifampin and dactinomycin and hydrolyzed by rnase. rna made by host-free chlamydiae consisted mainly of species of fewer than 800 bases in size, although 16s and 23s species were noted by agarose-gel electrophoresis. the hybridization of radiolabeled host-free rna to restriction fragments of the gene encoding the major outer membran ... | 1990 | 1698176 |
| variants of mta, the mouse maternally transmitted antigen, determined by three alleles of the extrachromosomal gene mtf. | two new forms of mta, the maternally transmitted antigen of the mouse, were defined by cytotoxic t lymphocytes. one is found in laboratory mice carrying the beta allele of the maternally transmitted factor, mtf, which were previously thought to be mta-negative. the other is associated with a new allele, mtf gamma, and is found in wild mice from toulouse. like the common form of mta, determined by the extrachromosomal mtf alpha, the new forms are also dependent on the a allele of hmt, a chromosom ... | 1990 | 1698881 |
| oysters, iron overload and vibrio vulnificus septicaemia. | a case of vibrio vulnificus septicaemia complicated by cutaneous leg ulceration is described. a 74 year old man with haemochromatosis and sideroblastic anaemia developed an acute febrile illness with cutaneous manifestations 24 hours after ingesting raw oysters. the presence of blistering should be considered an important clue to the diagnosis of vibrio vulnificus septicaemia, and this can facilitate prompt effective antimicrobial therapy. clinicians should be aware of this infection because of ... | 1990 | 2073206 |
| enhancement of virulence of two environmental strains of vibrio vulnificus after passage through mice. | the virulence of two environmental strains of vibrio vulnificus to iron-loaded adult mice was enhanced by passage through mice. estimates of 50% lethal dose values (ld50) determined by end point titration were reduced 100- and 1000-fold for the two strains. passage through mice also selected for the opaque colony type phase variation of v. vulnificus, reported by others to be more virulent to mice than a translucent colony type. | 1990 | 2076589 |
| permeability-enhancing action of vibrio vulnificus protease and its control system. | 1990 | 2101153 | |
| phenotypic evaluation of acapsular transposon mutants of vibrio vulnificus. | translucent, avirulent spontaneous phase variants of vibrio vulnificus mo6-24 reverted back to the original opaque, encapsulated phenotype under both in vivo and in vitro conditions. two translucent, acapsular mutants, which did not show phase variation, were constructed by using the transposon tn5 is50l::phoa (tnphoa). loss of capsule was accompanied by decreases in virulence, hydrophilicity, and serum resistance. the ability to utilize transferrin-bound iron for growth was lost in only one of ... | 1990 | 2160432 |
| kinetics of the reaction of prostaglandin h synthase compound ii with ascorbic acid. | the reduction of prostaglandin h synthase compound ii by ascorbic acid in the presence of diethyldithiocarbamate was studied in 0.1 m phosphate buffer (ph 8.0) at 4.0 +/- 0.5 degrees c, by rapid scan spectrometry and transient state kinetics. a saturation effect and nonzero intercept were observed in the plot of pseudo-first-order rate constant versus ascorbic acid concentration. the saturation behavior suggests formation of a complex between prostaglandin h synthase compound ii and ascorbic aci ... | 1990 | 2168160 |
| characterization of two low em forms of cytochrome a3 and their carbon monoxide complexes in mammalian cytochrome c oxidase. | evidence is presented for the existence of two forms of low-potential cytochrome a3. one appears to be similar to the low-spin form reported by nicholls, p., and v. hildebrandt (1978 biochem. j. 173:65-72) and wrigglesworth, j. m., j. elsden, a. chapman, n. van der water, and m. f. grahn (1988. biochim. biophys. acta. 936:452-464). it has a reduced soret peak near 428 nm and a prominent alpha peak near 602 nm. this form is seen when the enzyme is either supplemented with lipoprotein or incorpora ... | 1990 | 2168220 |
| role of oxygen free radical formation in the mechanism of menogaril resistance in multidrug resistant tumor cells. | the mechanisms of action and resistance to menogaril, a clinically active anthracycline antitumor drug, were evaluated in sensitive and doxorubicin-selected multidrug resistant human breast tumor (mcf-7) cell lines. while mcf-7/adrr cells were highly resistant (250-500-fold) to doxorubicin, they displayed only marginal resistance (10-fold) to menogaril. in contrast to doxorubicin, the mechanism of resistance to menogaril in these cells does not involve differential inhibition of dna synthesis as ... | 1990 | 2168296 |
| mechanisms underlying the stimulation of pth release by gppnhp in permeabilized bovine parathyroid cells. | we examined changes in camp and inositol phosphate metabolism to assess the contribution of the guanine nucleotide regulatory (g) protein(s) regulating adenylate cyclase and phospholipase c in mediating the stimulatory effects of gppnhp on pth release from permeabilized bovine parathyroid cells. to examine the role of gs, the g protein stimulating adenylate cyclase, and camp on pth release, permeabilized cells were incubated with either gppnhp or isoproterenol, and the effects of these agents on ... | 1990 | 2168660 |
| proton-nuclear magnetic resonance relaxation times in brain edema. | proton relaxation times of protein solutions, bovine brain, and edematous feline brain tissue were studied as a function of water concentration, protein concentration, and temperature. in accordance with the fast proton exchange model for relaxation, a linear relation could be established between r1 and the inverse of the weight fraction of tissue water. this relation also applied to r2 of gray matter and of protein solutions. no straightforward relation with water content was found for r2 of wh ... | 1990 | 2168666 |
| [a case of fatal sepsis due to vibrio vulnificus]. | a 66-year-old male with chronic alcoholic liver injury was admitted on july 27, 1986 to our hospital with complaints of high fever, convulsion and skin erythema. he had consumed raw fish 3 days before, and had a scratch wound over the right arm and left leg because he had slipped in a small stream in the woods the day before admission. he was already in shock state with sepsis of v. vulnificus and dic on admission. although the treatment with abpc, cp, caz, mino for sepsis, and heparin & antithr ... | 1990 | 2187037 |
| in vitro activity of ljc10,627, a new carbapenem antibiotic with high stability to dehydropeptidase i. | the in vitro activity of ljc10,627, a new carbapenem, was compared with those of imipenem and ceftazidime. ljc10,627 had broad-spectrum activity against gram-positive and gram-negative clinical isolates. the mics of this compound for 90% of members of the family enterobacteriaceae tested (mic90s), including strains resistant to ceftazidime, ranged from 0.1 to 25 micrograms/ml. ljc10,627 inhibited pseudomonas aeruginosa at an mic90 of 3.13 micrograms/ml; it thus was twofold more active than imipe ... | 1990 | 2203313 |
| massive rhabdomyolysis: a rare presentation of primary vibrio vulnificus septicemia. | 1990 | 2220890 | |
| abelson murine leukemia virus transforms preneoplastic emu-myc transgene-carrying cells of the b-lymphocyte lineage into plasmablastic tumors. | e mu-myc transgenic mice were back-crossed to balb/c mice up to back-cross generation 3. the offspring that included transgene-carrying and -negative mice in approximately equal proportions were randomly divided into 2 groups. thirty-four mice (group i) were treated with pristane, followed by a-mulv, and 40 (group ii) were injected with a-mulv alone. altogether, 16 lymphoid tumors developed in group i and 17 in group ii. nine of the tumors in group i and 4 in group ii appeared as ascitic tumors. ... | 1990 | 2228313 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats. | intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ... | 1990 | 2245800 |
| proteolytic processing of porcine big endothelin-1 catalyzed by cathepsin d. | cathepsin d, a candidate for endothelin-converting enzyme (ece), was allowed to act on porcine big endothelin-1 (big et-1, 1-39). the proteinase primarily cleaved the asp18-ile19 and trp21-val22 bonds of big et-1(1-39), with a optimum ph of 3.5. the mature et-1(1-21), generated by the cleavage between trp21 and val22, was subsequently degraded by removal of most of the c-terminal tripeptide (ile19-ile20-trp21). therefore, cathepsin d is by no means a specific ece, although the proteinase does cl ... | 1990 | 2268325 |
| a double-blind comparison of 0.5% bupivacaine and 0.75% ropivacaine administered epidurally in humans. | in an attempt to compare equipotent doses of epidurally administered bupivacaine and ropivacaine, 44 healthy patients, aged 18-70 years, undergoing lower extremity orthopedic procedures were studied in a randomized, double-blind manner. twenty-one patients received a single epidural injection of 20 ml 0.5% bupivacaine at the l23 or l34 interspace and 23 patients received 20 ml 0.75% ropivacaine in a similar manner. onset of and recovery from sensory anesthesia and motor block were recorded. no s ... | 1990 | 2271468 |
| a successfully treated case of vibrio vulnificus septicemia with shock. | vibrio vulnificus infection often causes serious or fatal disease. recently, in japan there have been numerous reports of vibrio vulnificus infection. here, we report a successfully treated case of vibrio vulnificus septicemia with shock, disseminated intravascular coagulation (dic) and necrotizing cellulitis in a middle-aged heavy drinker with chronic alcoholic liver disease. on reviewing 38 cases in japan including ours, the overall mortality rate was 68%. although the incidence is relatively ... | 1990 | 2273613 |
| incidence of vibrio vulnificus in northern new england water and shellfish. | vibrio vulnificus, an autochthonous inhabitant of the estuarine environment, was detected in water and oysters from the great bay estuary system of new hampshire and maine. previously, it had not been detected north of boston harbor on the east coast of the united states. v. vulnificus was detected in water and shellfish samples at five out of ten sites, and only in areas that were not open to recreational shellfishing. although samples were collected from may into december, v. vulnificus was on ... | 1990 | 2283033 |
| [bronchial hyperresponsiveness to histamine in guinea pig induced by intravenous administration of platelet activating factor]. | platelet activating factor (paf) has recently been demonstrated to play an important role in allergic diseases such as bronchial asthma. changes in airway wall thickness have recently been suggested to cause excess airway narrowing of asthma. in this study, the authors examined the bronchial hyperresponsiveness to histamine in guinea pig induced by intravenous administration of paf by measuring dynamic compliance and dynamic respiratory resistance. moreover, a new formula which can exclude the e ... | 1990 | 2290229 |
| generation of affinity for antithrombin iii by supplemental sulfation of heparin species with low affinity for the protein. | the tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin iii (mr 7,500-18,000; anti-clotting activity, 7 usp units/mg), having degrees of sulfate substitution at d-glucosamine and l-iduronic acid residues of glcns 0.786, glcn6s 0.628, and idoa2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in n,n-dimethylformamide at -10 degrees c for 1 h. both chemical and nmr spectroscopic analyses revealed ... | 1990 | 2292586 |
| vibrio vulnificus wound infection treated successfully with oral ciprofloxacin. | 1990 | 2299188 | |
| osteomyelitis caused by vibrio vulnificus. | 1990 | 2299219 | |
| polyvibrio infections: vibrio vulnificus and vibrio parahaemolyticus dual wound and multiple site infections. | 1990 | 2299220 | |
| vibrio vulnificus infections in the hand. report of three patients. | 1990 | 2303515 | |
| digestibility of cotton plant by-product diets for sheep at two levels of intake. | crossbred wethers (n = 48) were assigned randomly to 1 of 12 experimental diets, arranged in a 3 x 2 x 2 split plot to evaluate three levels of cotton plant by-product (25, 50 and 75% cpb), two levels of naoh treatment of cpb (0 and 4%) and two levels of monensin supplementation (0 and 30 ppm, dm basis) at two intake levels (70 and 90% of ad libitum). intake was a within-lamb variable. ad libitum intake was established over an initial 17-d adjustment period. feed intake and fecal output were rec ... | 1990 | 2312420 |
| primary vibrio vulnificus sepsis in kentucky. | vibrio vulnificus is associated with infection acquired during contact with sea water or with seafood, and is seldom suspected by physicians in noncoastal states. the ease of transportation of fresh raw seafood has facilitated this organism's capacity to produce disease in geographic areas in which it was previously unseen. we have reported a case of fatal vibrio vulnificus sepsis acquired from ingestion of fresh oysters in the inland united states. | 1990 | 2315791 |
| long-term clearance of inhaled uo2 particles from the pulmonary region of the rat. | this paper reports on an enriched uranium dioxide (uo2) mass clearance study undertaken with fischer-344 rats. the uo2 had a uranium (u) isotopic composition of 0.79% 234u, 92.8% 235u, 0.34% 236u and 6.06% 238u, by mass, with an alpha-particle activity of 1.91 bq micrograms-1. forty-six rats were exposed to an enriched uo2 aerosol that had an activity median particle aerodynamic diameter ranging from 2.7 to 3.2 microns. the rats were killed from 1 to 720 d post-inhalation (pi). the mass of enric ... | 1990 | 2323929 |
| casein and casein subunits in preterm milk, colostrum, and mature human milk. | human milk proteins have both nutritional and physiological roles for the breast-fed infant. while the biochemistry and developmental patterns for many whey proteins are well known, our knowledge of human casein and its subunits is still limited. we have recently developed a method to isolate casein from whey proteins in human milk and to separate the casein subunits by fast protein liquid chromatography. in this study we have applied this methodology to study the casein subunit pattern in prete ... | 1990 | 2358977 |
| blockade of inhibition in a pathway with dual excitatory and inhibitory action unmasks a capability for ltp that is otherwise not expressed. | long-term potentiation (ltp) can be readily elicited in a number of hippocampal pathways, but has not been seen in the dentate commissural pathway. the dentate commissural pathway is similar to the commissural/schaffer collateral projection to ca1 except that it produces powerful inhibition that occurs nearly concurrently with the excitation. the present study evaluates whether this inhibition prevents the pathway from expressing ltp. acute neurophysiological experiments were carried out in uret ... | 1990 | 2364294 |
| the cytolysin gene of vibrio vulnificus: sequence and relationship to the vibrio cholerae e1 tor hemolysin gene. | a cytolysin of ca. 56 kilodaltons has been suggested as a possible virulence factor in vibrio vulnificus infections. we sequenced the dna encoding cytolytic activity and found that the sequence contained two open reading frames, vvha and vvhb. vvha encoded the structural gene for the cytolysin and contained the n-terminal amino acid sequence previously reported for the protein. regions of the vvha gene showed homology to the structural gene for the vibrio cholerae e1 tor hemolysin. | 1990 | 2370116 |
| vibrio bacteremia in siriraj hospital. | from january 1983 to march 1988, 26 isolates of vibrio spp. were recovered from the blood of patients admitted to siriraj hospital. thirteen strains were identified as non 0-1 vibrio cholerae, 3 were vibrio vulnificus and 10 were vibrio spp. the medical records of 20 patients were available for clinical analysis. most of them were adult men with cirrhosis. clinical features included fever, abdominal pain, diarrhea, peritonitis, shock and skin lesions. some patients had a history of seafood consu ... | 1990 | 2380645 |
| white noise analysis of temporal properties in simple receptive fields of cat cortex. | we studied the linear and nonlinear temporal response properties of simple cells in cat visual cortex by presenting at single positions in the receptive field an optimally oriented bar stimulus whose luminance was modulated in a random, binary fashion. by crosscorrelating a cell's response with the input it was possible to obtain the zeroth-, first-, and second-order wiener kernels at each rf location. simple cells showed pronounced nonlinear temporal properties as revealed by the presence of pr ... | 1990 | 2390534 |
| increase in carcinoembryonic antigen release from cancer cells by combined treatment with retinoic acid and low-temperature hyperthermia. | the growth of a human gastric adenocarcinoma cell line, mkn-45, was inhibited and the amount of carcinoembryonic antigen (cea) in both the culture medium and the cell extract was increased in the presence of retinoic acid at a concentration of 75 (1.5x)-125 microm (1.9x), which did not substantially affect cell survival. treatment using a combination of retinoic acid (125 microm) and low-temperature hyperthermia (40 degrees c, 30 min) was more effective in increasing cea compared with retinoic a ... | 1990 | 2394926 |
| are separable aromatase systems involved in hormonal regulation of the male brain? | in vitro study of testosterone (t) metabolism shows that formation of estradiol-17 beta (e2) is regionally specific within the preoptic area (poa) of the male ring dove. the poa is known to be involved in the formation of e2 required for specific components of male sexual behavior. two sub-areas of high aromatase activity, anterior (apoa) and posterior preoptic (ppoa) areas, have been identified. aromatase activity is higher in apoa than in ppoa. the aromatase activity within the apoa is also mo ... | 1990 | 2394989 |
| metabolism of indium-111-labeled murine monoclonal antibody in tumor and normal tissue of the athymic mouse. | we have studied the fate of radiometal metabolism in vivo by analyzing the molecular form of radiolabeled anti-carcinoembryonic antigen (cea) in tissues. athymic mice bearing colonic tumor xenografts were injected i.v. with 111in-isothiocyanate-benzyl-dtpa-(scn-bz-dtpa) igg or 111in-(scn-bz-dtpa) f(ab')2, and then killed daily for up to four days. liver, kidney, and tumor were extracted and the supernatants, plasma and urine samples were analyzed by hplc, itlc, hpiec and sds-page. by hplc, the a ... | 1990 | 2395019 |
| phrenic motoneuron morphology in the neonatal rat. | the morphology of neonatal rat phrenic motoneurons was studied following retrograde labeling with horseradish peroxidase, which resulted in golgi-like fills of phrenic motoneuron somata and dendrites. at birth, these neurons have well-developed dendritic trees with many characteristics described for phrenic motoneurons in the adult rat. the dendrites form tightly fasciculated bundles that emerge from the phrenic nucleus primarily along four axes: ventromedial, ventrolateral, dorsolateral, and ro ... | 1991 | 1716267 |
| alpha-macroglobulin-like plasma inactivator for vibrio vulnificus metalloprotease. | the metalloprotease produced by vibrio vulnificus (vvp) is known to be quickly inactivated by plasma proteins which belong to the class of alpha-macroglobulins in vitro at a molar ratio of 1:1. but the in vivo potential of the inactivators has not been studied. macroalbumin (ma), a member of alpha-macroglobulins in guinea pig plasma, was found to inactivate vvp by means of physical entrapment in vitro. in vivo actions of vvp, permeability-enhancing and hemorrhagic actions, were greatly augmented ... | 1991 | 1723411 |
| identification of vibrio vulnificus o serovars with antilipopolysaccharide monoclonal antibody. | a serotyping scheme for vibrio vulnificus predicated on the detection of lipopolysaccharide (lps) antigens is proposed. the serovar o typing scheme used to type v. vulnificus employs polyclonal antisera raised in rabbits immunized with heat-killed whole-cell vaccines. polyclonal typing sera produced in this manner cross-react with heterologous strains. affinity purification of polyclonal antisera with lps affinity columns resolved some of these cross-reactions; however, affinity-purified polyclo ... | 1991 | 1761690 |
| formation of nonculturable vibrio vulnificus cells and its relationship to the starvation state. | entry into the viable but nonculturable state by the human bacterial pathogen vibrio vulnificus in artificial seawater microcosms was studied. in contrast to the long-term culturability exhibited by cells incubated under these starvation conditions at room temperature, cells exposed to a temperature downshift to 5 degrees c exhibited an immediate decrease in culturability. cells incubated at low temperature exhibited a morphological change from rods to cocci but demonstrated no reductive divisio ... | 1991 | 1768138 |
| use of the polymerase chain reaction in detection of culturable and nonculturable vibrio vulnificus cells. | vibrio vulnificus is a human pathogen associated with consumption of raw oysters. during the colder months the organism apparently enters a viable but nonculturable state and thus cannot be cultured by ordinary bacteriological methods. for this reason, another means of detecting this bacterium is necessary. in the present study we utilized the polymerase chain reaction (pcr) to detect v. vulnificus dna, thus eliminating the problem of nonculturability. dna from both culturable and nonculturable ... | 1991 | 1768140 |
| analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development. | using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ... | 1991 | 1769342 |
| vibrio vulnificus. hazard on the half shell. | vibrio vulnificus is an extremely invasive gram-negative bacillus that causes bacteremia and shock. it should be suspected in any patient who is immunocompromised or has liver disease or hemochromatosis. reduced gastric acidity may also increase the risk of infection if a patient presents with a history of ingesting raw shellfish (especially oysters) or trauma in brackish waters and skin lesions. patients most commonly present with one of three clinical syndromes: primary septicemia, wound infec ... | 1991 | 1771878 |
| a lethal leviathan--vibrio vulnificus. | 1991 | 1771890 | |
| vibrio vulnificus peritonitis. a unique case. | louisiana is justifiably famous for the seafood harvested from its coastal waters. unfortunately, undesirables accompany this harvest. the author presents a case of peritonitis due to vibrio vulnificus which followed ingestion of raw oysters. | 1991 | 1779190 |
| physiological effects of the lipopolysaccharide of vibrio vulnificus on mice and rats. | vibrio vulnificus is a pathogenic, marine, gram-negative bacterium which commonly enters and infects humans via open wounds or the ingestion of raw seafood. the lipopolysaccharide (lps) of v. vulnificus has recently been characterized, but the biological activity of this putative endotoxin is unknown. three treatment groups were used to test its activity: saline (negative control), the lps of salmonella typhimurium (a known endotoxin), and the lps of v. vulnificus. whereas, intravenous injection ... | 1991 | 1779875 |
| [biological characteristics of cultured papilla cells--localization of androgen receptors and chemotactic factor(s) for epithelial cells]. | we have reported that cultured papilla cells (pcs) grown by isolation and cultivation of human hair papillae show some biological characteristics. in the present report, some important biological characteristics of pcs are showed. 1) localization of androgen receptors on pcs localization of androgen binding protein in pcs was examined. cytochemical staining of pcs using dht-peroxidase conjugate gave positive reactions in the niclei of pcs originating from scalp and axilla-dermal papillae. these ... | 1991 | 1782180 |