Publications

necrotizing fasciitis due to vibrio alginolyticus in an immunocompetent patient.	we describe a patient with a history of asthma and remote use of steroids the development of necrotizing fascitis due to vibrio alginolyticus after an injury from a coral reef during bathing in the caribbean sea off colombia. the patient recovered with aggressive surgical debridement and antibiotics.	2003	12843111
molecular cloning, expression of orange-spotted grouper goose-type lysozyme cdna, and lytic activity of its recombinant protein.	lysozyme acts as a non-specific innate-immunity molecule against the invasion of bacteria pathogens. a leukocyte cdna library of orange-spotted grouper epinephelus coioides was constructed and the goose-type (g-type) lysozyme cdna was isolated. the complete cdna consists of an open reading frame of 585 bp encoding a protein of 194 amino acids. this protein shows a 72.2% amino acid sequence identity with the flounder g-type lysozyme. similar to most other species, the glu catalytic residue in g-t ...	2003	12911059
hepatic cholangiocarcinoma and testicular mesothelioma in a wild-caught blue shark, prionace glauca (l.).	an adult male blue shark, prionace glauca (l.), caught in july 2000 by a recreational fisherman off long island, new york, usa, had a retained fishing hook from a previous capture. the hook penetrated the gastric wall and lacerated the right liver lobe. macroscopic lesions consisted of transmural gastritis and peritonitis. alteromonas sp. and vibrio alginolyticus were isolated from the peritoneal fluid. in addition, a well delineated, sessile mass was found on the otherwise normal serosa of the ...	2003	12962210
oral administration of formalin-inactivated cells of aeromonas hydrophila a3-51 controls infection by atypical a. salmonicida in goldfish, carassius auratus (l.).	there has been increasing interest in the use of probiotics in aquaculture to control fish diseases (e.g. douillet langdon 1994; gildberg, mikkelsen, sandaker & ringo 1997; kennedy, tucker, neidic, vermeer, cooper, jarrell & sennett 1998; deschrijver & ollevier 2000; robertson, o'dowd, burrells, williams & austin 2000), and a diverse range of gram-positive (such as carnobacterium inhibens) (robertson et al. 2000) and gram-negative bacteria, including vibrio alginolyticus (garriques & arevalo 199 ...	2003	12962220
misidentification of aeromonas veronii biovar sobria as vibrio alginolyticus by the vitek system.	to find the cause of misidentification of aeromonads when using the vitek system.	2003	12969502
retention of virulence in viable but non-culturable halophilic vibrio spp.	the viable but non-culturable (vbnc) forms of two environmental strains of vibrio alginolyticus 1 and vibrio parahaemolyticus 66 and one strain of v. parahaemolyticus atcc 43996 showing virulence characteristics (hemolysin production, adhesive and/or cytotoxic ability, in vivo enteropathogenicity) were obtained by culturing bacteria in a microcosm consisting of artificial sea water (asw) and incubating at 5 degrees c with shaking. every 2 days, culturability of the cells in the microcosm was mon ...	2003	14580971
imprisonment in a death-row cell: the fates of microbes entrapped in the limulus blood clot.		2003	14583530
description of vibrio alginolyticus infection in cultured sepia officinalis, sepia apama, and sepia pharaonis.		2003	14583547
quinoxalin-2-ones. part 5. synthesis and antimicrobial evaluation of 3-alkyl-, 3-halomethyl- and 3-carboxyethylquinoxaline-2-ones variously substituted on the benzo-moiety.	a new series of 3-alkyl-, 3-trifluoromethyl-, 3-carboxyethyl- and 3-bromomethylquinoxaline-2-ones and 2,3-bis(bromomethyl)quinoxalines bearing cl, cf3, morpholine on the benzo-moiety, were synthesised and submitted to a preliminary in vitro evaluation for antibacterial and anticandida activities. results of the screening showed that compounds 9b, 14b and 19b (mic=62.5 microg/ml) and 10b (mic=15.6 microg/ml) were the most active against vibrio alginolyticus.	2003	14630235
intraspecific characterization of vibrio alginolyticus isolates recovered from cultured fish in spain.	intraspecific differentiation and characterization of vibrio alginolyticus strains isolated from cultured fish in spain.	2003	14633040
accuracy of six commercially available systems for identification of members of the family vibrionaceae.	six commercially available bacterial identification products were tested with vibrio alginolyticus (12 strains), v. cholerae (30 strains), photobacterium (vibrio) damselae (10 strains), v. fluvialis (10 strains), v. furnissii (4 strains), v. hollisae (10 strains), v. metschnikovii (9 strains), v. mimicus (10 strains), v. parahaemolyticus (30 strains), and v. vulnificus (10 strains) to determine the accuracy of each system for identification. the products included api 20e, crystal e/nf, microscan ...	2003	14662957
vibrio alginolyticus associated with white spot disease of penaeus monodon.	in february 2000, white spot disease outbreaks occurred among cultured penaeus monodon in extensive shrimp farms on the southwest coast of india. bacteria were isolated from infected shrimp that showed reddish body coloration and white spots in the cuticle. the isolates were screened on thiosulfate citrate bile salt sucrose (tcbs) agar plates for the selection of vibrio species. the primary isolate (qs7) was characterized as v. alginolyticus based on morphological, biochemical and physiological ...	2003	14735933
[occupational exogenous siderosis of the skin (author's transl)].		2003	151604
[fate of a lavsan strip used for stenosing the small intestine].		2003	142861
an enzyme histochemical study of non-hodgkin's lymphoma and allied disease.	the enzyme activity for alpha-naphthyl-acetate esterase, naphthol-as-acetate esterase, naphthol-as-d-chloroacetate esterase, acid phosphatase, l(+)-tartrate-resistant acid phosphatase, adenosine triphosphatase, and 5'-nucleotidase was examined on the neoplastic cells of giant follicular lymphoblastoma, the so-called reticulum cell sarcoma and sézary syndrome. the neoplastic cells of giant follicular lymphoblastoma showed distinct activity for adenosine triphosphatase and 5'-nucleotidase, and tho ...	2003	145325
[state of the pituitary-adrenal system in burns].		2003	201039
pelvic displacement of a cardiac pacemaker following trauma. a case report.	the rate migration of an implanted cardiac pacemaker from the subrectus area anterior to the peritoneum to the pelvis following a motor vehicle accident is reported. discovery of dislodgement was made by observing a marked increase of pacemaker impulse amplitude in the three standard limb leads as measured on an oscilloscope.	2003	152472
[gastroschisis and omphalocele. treatment and results].		2003	150087
[development of asphyxia in hematogenic osteomyelitis of the mandible in a newborn infant].		2003	283596
[study of left ventricular function with quantitative angiocardiographic methods].		2003	161533
the independent living centre.		2003	161503
bacterial dynamics associated with algal antibacterial substances during post harvest desiccation process of sargassum stolonifolium phang et yoshida.	brown algae of genus sargassum are known to produce relatively higher amount of alginic acid. optimal extraction of this algalcolloid for local consumption requires in-depth studies on post-harvest treatment of the algal fronds. present investigation endeavors to establish the dynamics and inter-relationship of moisture content and bacteria found on the surface of the alga and alginic acid content during post-harvest desiccation of sargassum stolonifolium phang et yoshida. harvested fronds were ...	2003	15248481
unusual marine pathogens causing cellulitis and bacteremia in hemodialysis patients: report of two cases and review of the literature.	cellulitis in immunocompetent hosts is usually caused by skin organisms and responds to oral antibiotics. in immunocompromised hosts, such as end-stage renal disease (esrd) patients, unusual organisms with unpredictable pathogenicity can cause infections. we present two cases with isolates that are usually encountered in coastal waters. the first case describes a 71-year-old african-american esrd patient who presented with bacteremia secondary to a wound infection after exposure to flood waters ...	2003	19379389
characteristics of na-dependent respiratory chain in vibrio anguillarum, a fish pathogen, in comparison with other marine vibrios.	the activity of membrane-bound nadh oxidase of vibrio anguillarum m93 (serotype j-o-1), which causes vibriosis in freshwater area was activated by na(+) in the same manner as other marine vibrios. however, in addition to na(+), k(+) was also found to positively enhance the nadh oxidase activity of strain m93. this tendency has not been recognized in other marine vibrios. furthermore, the na(+)-dependent nadh oxidase of strain m93 required less na(+) (0.1 m) for its maximum activity than those of ...	2003	19719639
interaction of pomb with the third transmembrane segment of poma in the na+-driven polar flagellum of vibrio alginolyticus.	the marine bacterium vibrio alginolyticus has four motor components, poma, pomb, motx, and moty, responsible for its na(+)-driven flagellar rotation. poma and pomb are integral inner membrane proteins having four and one transmembrane segments (tms), respectively, which are thought to form an ion channel complex. first, site-directed cys mutagenesis was systematically performed from asp-24 to glu-41 of pomb, and the resulting mutant proteins were examined for susceptibility to a sulfhydryl reage ...	2004	15292129
[case report: otitis due to vibrio alginolyticus].	infections caused by vibrio alginolyticus is generally disregarded because of the fact that it is an unusual pathogen for humans. in this report, a 57 years old male patient with otitis media has been presented. intense and pure isolation of v. alginolyticus was achieved from the ear discharge sample. according to the history, it was detected that he was on vacation at mediterranean coast three weeks ago, thus the transmission of the bacteria is possibly via seawater. the isolate was found sensi ...	2004	15293914
the immune response of white shrimp litopenaeus vannamei and its susceptibility to vibrio alginolyticus under nitrite stress.	the white shrimp litopenaeus vannamei were challenged with tryptic soy broth (tsb)-grown vibrio alginolyticus at a dose of 1 x 10(6) colony-forming units (cfu) shrimp(-1), and then placed in water containing concentrations of nitrite-n at 0 (control), 1.12, 5.15, 11.06 and 21.40 mg l(-1). mortality of shrimp in 5.15, 11.06 and 21.40 mg l(-1) was significantly higher than those in the control solution after 48-168 h. l. vannamei that had been exposed to control, 0.98, 4.94, 9.87 and 19.99 mg l(-1 ...	2004	15312659
effect of copper sulfate on the immune response and susceptibility to vibrio alginolyticus in the white shrimp litopenaeus vannamei.	white shrimp litopenaeus vannamei (boone) held in 35 per thousand seawater were challenged with vibrio alginolyticus at a dose of 3 x 10(5) colony-forming units (cfu) shrimp(-1), and then placed in water containing concentrations of cu2+ at 0 (control), 1, 5, 10 and 20 mg l(-1). mortality of shrimp in 5, 10 and 20 mg l(-1) cu2+ was significantly higher than those in 1 mg l(-1) cu2+ and the control solution after 24-96 h. in another experiment, l. vannamei which had been exposed to control, 1, 5, ...	2004	15313510
structural and functional differences of litopenaeus vannamei crustins.	penaeid crustins were described in litopenaeus vannamei and l. setiferus as proteins belonging to an antibacterial peptide family with similar sequences but different sizes. six crustin-coding clones were isolated from a cdna library from l. vannamei hemocytes, sequenced and compared. two different isoforms (named i and p) were found, based on two nucleotide differences that produce one change in amino acid sequence (ile/pro). other single differences in nucleotide sequences were also noted, but ...	2004	15325343
digestive pathology of sea turtles stranded in the canary islands between 1993 and 2001.	digestive lesions were observed in 84 of 136 sea turtles (128 caretta caretta, four chelonia mydas and four dermochelys coriacea) stranded in the canary islands between january 1993 and december 2001. in the oral cavity ulcerative and necropurulent stomatitis were the most frequently observed lesions, and in the oesophagus ulcerative and fibrinous oesophagitis, and traumatic oesophageal perforation were most frequently observed; all these lesions were mainly associated with the ingestion of fish ...	2004	15357377
two novel species of tetrodotoxin-producing bacteria isolated from toxic marine puffer fishes.	out of eight dominant discrete bacterial colonies isolated and purified from the toxic marine puffer fishes collected in hong kong waters, two novel species of non-sporing, non-acid-fast and chemoorganotrophic bacteria capable of producing tetrodotoxin (ttx, a potent non-protein neurotoxin), as well as one previously reported and confirmed ttx-producing bacterium. they were identified as microbacterium arabinogalactanolyticum, serratia marcescens and vibrio alginolyticus, respectively, all of wh ...	2004	15501290
functional characterization of two type iii secretion systems of vibrio parahaemolyticus.	vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. recent genome sequencing of the clinical v. parahaemolyticus strain rimd2210633 identified two sets of genes for the type iii secretion system (ttss), ttss1 and ttss2. here, we constructed a series of mutant strains from rimd2210633 to determine whether the two putative ttss apparatus are functional. the cytotoxic activity of mutant strains having a deletion in one of the ttss1 genes wa ...	2004	15501799
vibrio alginolyticus infection in the white shrimp litopenaeus vannamei confirmed by polymerase chain reaction and 16s rdna sequencing.	a gram-negative, rod-shaped bacterium identified as vibrio alginolyticus was isolated from diseased litopenaeus vannamei (also called penaeus vannamei) in taiwanese culture ponds. the diseased shrimp displayed poor growth, anorexia, inactivity, reddish pleural borders of antennae, uropods and telson, opaque and whitish musculature, and mortality. in histological preparations, melanized hemocytic granulomas were observed in the connective tissue around hemal sinuses together with hemocytic aggreg ...	2004	15584425
modulated heat shock protein expression during pathogenic vibrio alginolyticus stress of sea bream.	the effects of infective vibrio alginolyticus and its extracellular product (ecp) on host fish function are not well understood. in this study a partial biochemical characterization of the ecp from an infective strain of v. alginolyticus isolated from diseased silver sea bream sparus sarba was achieved and the effects of live v. alginolyticus and ecp on hepatic heat shock protein (hsp) expression was compared. the ecp fraction was found to contain several hydrolytic enzymes including both haemol ...	2004	15672876
a four-kazal domain protein in litopenaeus vannamei hemocytes.	a cdna clone coding for a kazal protein was isolated from a cdna library of litopenaeus vannamei hemocytes. the full-length cdna sequence is 1.32 kb long and encodes a 24 kda protein. four kazal domains, 43-46 residues long, were detected in the deduced primary structure. apparently, the first and second domains do not have inhibitory activity, as predicted by the sequence between the second and third cys. the third and fourth domains have the sequences cpeiyapvc and cpknyrpvc, indicating that t ...	2004	15707660
vaginal epithelial changes associated with in utero des exposure.	to determine the true prevalence of vaginal epithelial changes in des-exposed offspring, the national cancer institute funded the desad project, a study designed to follow up these women for 4 years and to determine the prevalence of cancer and various other abnormalities in des-exposed women. 3339 women have constituted the project study cohort as of 12/31/76. each woman in the desad project undergoes breast, pelvic and colposcopic examinations, as well as vaginal and cervical cytology. details ...	2004	523034
congressman normal mineta: keeping tabs on the clean water act. interview by donald feliciano.		2004	522208
the problem with vitamin b12 testing.		2004	522179
an analysis of nonconfirmation rates. experiences of a surgical second opinion program.	a review was conducted of the first 12 months' experience with a voluntary second opinion surgery consultation program. the program covers approximately 950,000 persons: employees of the city of new york, retirees, and their dependents. on a simple yes or no basis, the program's surgical nonconfirmation rate was 25%. more detailed analysis of consultants' narrative suggestions for patient care showed that a firm rejection of surgical intervention occurred in only 8% of the cases reviewed. noncon ...	2004	490854
[inhibition of postoperative catabolism with bradykinin].		2004	421629
bloody pleural effusion associated with an intralobar pulmonary sequestration.	a 50-year-old male cigarette smoker with a posterior parenchymal lung mass developed a bloody, exudative pleural effusion. pathologic examination of the resected lung confirmed the preoperative diagnosis of an intralobar pulmonary sequestration.	2004	421588
transventricular blood vessels in the third ventricle of the armadillo brain.	transventricular filaments were observed with scanning electron microscopy on the dorsolateral wall of the infundibular recess of the third ventricle of the armadillo brain. two to seven transventricular filaments per animal were present in 6 of 18 animals. there were two types of transventricular filaments, ciliated and bare. using transmission electron microscopy, we determined that these filaments consisted of a single, central capillary surrounded by ciliated ependymal cells and a small accu ...	2004	443562
a single wap domain-containing protein from litopenaeus vannamei hemocytes.	a cdna clone coding for a single wap domain (swd) protein was isolated from a hemocyte cdna library of litopenaeus vannamei. the full-length cdna sequence is 0.4kb long and encodes a 93-amino acid protein. using this sequence as a probe a similar clone coding for a 92-amino acids protein was found in a cdna library from penaeus monodon hemocytes. the mrna size was confirmed by northern blot as well as that gene is expressed in hemocytes, but not in hepatopancreas. mrna levels of the shrimp swd p ...	2004	14741689
vibrio hispanicus sp. nov., isolated from artemia sp. and sea water in spain.	three gram-negative, small, motile, rod-shaped bacteria were isolated from artemia sp. and sea water in barcelona, spain, during 1990 and 1991. they were fermentative, oxidase-positive, sensitive to vibriostatic agent o/129, arginine dihydrolase-positive, lysine and ornithine decarboxylase-negative and grew in the absence of nacl. they differed from phenotypically related species by their ability to grow at 4 degrees c and utilize l-rhamnose. cloning of the 16s rrna gene of the type strain produ ...	2004	14742490
a k+ yptake protein, trka, is required for serum, protamine, and polymyxin b resistance in vibrio vulnificus.	vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the world. to identify the genes required for resistance to human serum, we constructed a library of transposon mutants of v. vulnificus and screened them for hypersensitivity to human serum. here we report that one of the isolated serum-susceptible mutants had a mutation in an open reading frame identified as trka, a gene encoding an amino acid seq ...	2004	14742502
isolation and characterization of pathogenic vibrio alginolyticus from diseased cobia rachycentron canadum.	outbreaks of serious mortality among cultured juvenile cobia rachycentron canadum l. (weighing 8-10 g) characterized by lethargy, dark skin and ascites in the peritoneal cavity while some fish possessing damaged eyes occurred in july and august of 2001 in taiwan. fifteen motile bacterial strains were isolated from head kidney and/or the ascites on tryptic soy agar supplemented with 1% nacl (tsa1) and/or thiosulphate citrate bile salt (tcbs) sucrose agar plates during the two outbreaks. all the i ...	2004	14768024
in vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus.	the selection of probiotics for aquaculture is usually based on their antagonism towards pathogens. however, other criteria such as growth, attachment to intestinal mucus and production of beneficial compounds should also be considered. we suggest a protocol for the isolation and selection of potential probiotic bacteria based on their in vitro growth characteristics and propose a ranking index (ri) to screen potential aquaculture probionts. we suggest that the lag period and doubling time are t ...	2004	14769479
synthesis, anti-mycobacterial, anti-trichomonas and anti-candida in vitro activities of 2-substituted-6,7-difluoro-3-methylquinoxaline 1,4-dioxides.	a new series of 23 6,7-difluoro-3-methyl-2-phenylthio/phenylsulfonyl/phenylsulfinyl/benzylamino/phenylamino-quinoxaline 1,4-dioxides variously substituted in the phenyl moiety, was synthesized and submitted to in vitro evaluation for anti-mycobacterial, anti-trichomonas, anti-candida, anti-mycoplasma and antibacterial activities. in anti-mycobacterial assays, several compounds resulted active (mic90 = 2.0-4.0 microg/ml) against mycobacterium tuberculosis h37rv. anti-trichomonas screening showed ...	2004	14987828
multimeric structure of the poma/pomb channel complex in the na+-driven flagellar motor of vibrio alginolyticus.	it is known that poma and pomb form a complex that functions as a na(+) channel and generates the torque of the na(+)-driven flagellar motor of vibrio alginolyticus. it has been suggested that poma works as a dimer and that the poma/pomb complex is composed of four poma and two pomb molecules. poma does not have any cys residues and pomb has three cys residues. therefore, a mutant pomb (pomb(cl)) whose three cys residues were replaced by ala was constructed and found to be motile as well. we car ...	2004	14999008
[investigation of the bacteria in the seawater of xisha in the south china sea and their antibiotic sensitivity profile].	to investigate the distribution and antibiotic sensitivity of the bacteria in the seawater of xisha in the south china sea.	2004	15041561
isolation of vibrio alginolyticus sodium-driven flagellar motor complex composed of poma and pomb solubilized by sucrose monocaprate.	the polar flagella of vibrio alginolyticus have sodium-driven motors, and four membrane proteins, poma, pomb, motx and moty, are essential for torque generation of the motor. poma and pomb are believed to form a sodium-conducting channel. this paper reports the purification of the motor complex by using sucrose monocaprate, a non-ionic detergent, to solubilize the complex. plasmid pkj301, which encodes intact poma, and pomb tagged with a c-terminal hexahistidine that does not interfere with pomb ...	2004	15073300
effect of ammonia on the immune response of white shrimp litopenaeus vannamei and its susceptibility to vibrio alginolyticus.	growth of vibrio alginolyticus was not affected by tsb medium containing ammonia-n concentration in the range of 0-20 mg l(-1). white shrimp litopenaeus vannamei (7-12 g in the intermolt stage) were challenged with v. alginolyticus, which had been incubated for 24 h in the tsb medium containing different concentrations of ammonia-n (0, 1, 5. 10 and 20 mg l(-1)). there was no significant difference in cumulative mortality for shrimp incubated in the tsb medium containing 0, 1, 5, 10 and 20 mg l(- ...	2004	15123301
the immune response of the white shrimp litopenaeus vannamei and its susceptibility to vibrio infection in relation with the moult cycle.	the white shrimp litopenaeus vannamei (8.0-14.4 g) was examined for haemocyte count, phenoloxidase activity, respiratory burst (release of superoxide anion), phagocytic activity, and clearance efficiency to the pathogen vibrio alginolyticus in relation with moult cycle (postmoult, a, b; intermoult, c; premoult, d(0)/d(1)d(2)/d(3)). granular cells were the highest at c and d(0)/d(1)stage, and the lowest at a stage. hyaline cells and thc (total haemocyte count) were higher at c stage, but lower at ...	2004	15123319
otitis media associated with vibrio alginolyticus in a child with pressure-equalizing tubes.	vibrio alginolyticus is an unusual cause of otitis media. infection usually occurs in the presence of a chronically perforated eardrum or patent pressure-equalizing tube. infection with v. alginolyticus can occur after even mild, brief exposure to seawater, and the interval between exposure to seawater and onset of clinical infection can be prolonged.	2004	15131479
the immune stimulatory effect of sodium alginate on the white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus.	the total haemocyte count (thc), differential haemocyte count (dhc), phenoloxidase activity, respiratory burst (release of superoxide anion), superoxide dismutase activity, phagocytic activity and clearance efficiency to the pathogen vibrio alginolyticus were measured when the white shrimp litopenaeus vannamei (9.4-11.3 g) were injected individually with sodium alginate at 10, 20 or 50 microg g(-1). no significant differences in thc, dhc and superoxide dismutase activity were observed among the ...	2004	15145416
competition for attachment of aquaculture candidate probiotic and pathogenic bacteria on fish intestinal mucus.	probiotics for aquaculture are generally only selected by their ability to produce antimicrobial metabolites; however, attachment to intestinal mucus is important in order to remain within the gut of its host. five candidate probiotics (ap1-ap5), isolated from the clownfish, amphiprion percula (lacepéde), were examined for their ability to attach to fish intestinal mucus and compete with two pathogens, aeromonas hydrophila and vibrio alginolyticus. two different radioactive isotopes were used to ...	2004	15189372
effect of vibrio alginolyticus on larval survival of the blue mussel mytilus galloprovincialis.	the effect of increasing concentrations of vibrio alginolyticus on survival of mytilus galloprovincialis larvae was studied in a 48 h static bioassay in 1 l glass bottles. five bacterial densities were tested ranging from 10(2) to 10(6) bacteria ml(-1). larval survival and normality (veliger larvae with the typical d-shape) were evaluated after 48 h. an inverse relationship between bacterial concentration and larval survival and normality was observed. in spite of high larval survival (79%) unde ...	2004	15212277
in vitro inhibition of microbial flora of fish by nisin and lactoperoxidase system.	to test the antimicrobial effects of nisin and lactoperoxidase system (lp system) against sardines flora. this study is part of a programme designed to investigate the preservability of fish using these inhibitors as potential biopreservatives.	2004	14687217
[choledochoplasty in the treatment of cholelithiasis].		2004	516291
homogeneous immunoglobulins in the serum of irradiated and bone marrow reconstituted mice: the role of thymus and spleen.	the influence of thymectomy and splenectomy on the frequency and class distribution of homogeneous immunoglobulins (h-ig) in serum was studied in lethally irradiated (dba/2 x c57bl/rij)f1 mice reconstituted with syngeneic bone marrow. during four follow-up periods in the first 9 months after transplantation, the sham-operated controls and splenectomized animals developed transient h-ig in an average frequency of 14.2 and 15.7% respectively. there were no marked differences in the incidence of h- ...	2004	387578
[a rapid method for the determination of urinary pregnandiol using sephadex lh-20 chromatography].		2004	520858
a method for monitoring the treatment of schizophrenics in the community.	a trial was carried out over twelve months of a method for monitoring the care of a large number of schizophrenic patients in the community. the method has been demonstrated to be practical in use, economical of resources and potentially relevant to a number of chronic disorders.	2004	444792
a simple centrifuge column for desalting protein solutions.		2005	543535
pathophysiological studies on schizophrenia with special reference to homovanillic acid concentration in cerebrospin fluid.		2005	540184
[antidepressive treatment with nortriptyline].		2005	1233907
a flow calorimeter for assay of hormone- and metabolite-induced changes in steady-state heat production by tissue.		2005	543542
management of pathological fractures at the mayo clinic.		2005	1234120
serum-free media and cell cultures. introductory remarks.		2005	1367047
serum-free media and cell cultures. introductory remarks.		2005	1367047
diffusion in immobilized-cell gels.	the relationship between the diffusion coefficient, the effective diffusion coefficient and the partition coefficient for a solute in a cell-containing gel is discussed. the use of correlation equations that are based on some kind of physical model is recommended when the effect of cell concentration on diffusion is interpreted.	2005	1367033
diffusion in immobilized-cell gels.	the relationship between the diffusion coefficient, the effective diffusion coefficient and the partition coefficient for a solute in a cell-containing gel is discussed. the use of correlation equations that are based on some kind of physical model is recommended when the effect of cell concentration on diffusion is interpreted.	2005	1367033
practical methods for preservation of cultured cells by freezing.		2005	1366945
low-income widows and other aged singles.	based on data collected in a nationwide survey in late 1973, this report describes the living conditions, health, social activity, and attitudes of unmarried older persons at low to middle income levels--that is, below $5,000 per year. although most previous research has focused on the special situation of widows, the current findings indicate that lower income widowers and divorced/separated/never-married persons generally experience many of the same problems as the widows and respond to them i ...	2005	545715
[infections in immunosuppressed patients].		2005	544237
wheezy bronchitis in children; etiopathogenetic role of non-infectious agents.		2005	1305512
diphtheria toxin entry: protein translocation in the reverse direction.		2005	3072713
[duodenal reflux and gastritis after billroth i gastric resection (author's transl)].	endoscopic and biopsy studies were undertaken in 92 patients who had undergone partial gastric resection (end-to-end gastroduodenostomy, billroth i) one to seven years previously. in addition, the amound of duodeno-gastric reflux was evaluated by measuring bromsulphalein excretion in gastric juice. atrophic gastritis occurred in 36%, while superficial gastritis was noted in 49% of patients. the degree and severity of the inflammatory changes was most marked near the anastomosis. the severity of ...	2005	1183346
platelet monoamine oxidase activity in patients with chronic schizophrenic illnesses untreated by neuroleptic drugs [proceedings].		2005	1234010
effect of a mixture of cryoprotectants in attaining liquid nitrogen survival of callus cultures of a tropical plant.		2005	544179
the immunostimulatory effect of hot-water extract of gracilaria tenuistipitata on the white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus.	the total haemocyte count (thc), phenoloxidase activity, respiratory burst, superoxide dismutase (sod) activity, phagocytic activity and clearance efficiency to the pathogen vibrio alginolyticus were examined in the white shrimp litopenaeus vannamei (10.3+/-1.5 g) injected individually with hot-water extract of gracilaria tenuistipitata at 4 or 6 microg g-1. l. vannamei receiving hot-water extract of g. tenuistipitata at either dose increased significantly its thc, phenoloxidase activity, and re ...	2005	15752651
diseases and causes of mortality among sea turtles stranded in the canary islands, spain (1998-2001).	this paper lists the pathological findings and causes of mortality of 93 sea turtles (88 caretta caretta, 3 chelonia mydas, and 2 dermochelys coriacea) stranded on the coasts of the canary islands between january 1998 and december 2001. of these, 25 (26.88%) had died of spontaneous diseases including different types of pneumonia, hepatitis, meningitis, septicemic processes and neoplasm. however, 65 turtles (69.89%) had died from lesions associated with human activities such as boat-strike injuri ...	2005	15759796
the protective effect of chitin and chitosan against vibrio alginolyticus in white shrimp litopenaeus vannamei.	white shrimp, litopenaeus vannamei, which had been injected with chitin at 4, 6 and 8 microg g(-1) or chitosan at 2, 4 and 6 microg g(-1), were challenged with pathogen vibrio alginolyticus at 2 x 10(6) colony-forming units (cfu) shrimp(-1) and then placed in seawater of 34 per thousand. the survival of shrimp that received chitin or chitosan at either dose was significantly higher than that of control shrimp after 1 day, and at the termination of the experiment (6 days after the challenge). in ...	2005	15820121
genetic heterogeneity among vibrio alginolyticus isolated from shrimp farms by pcr fingerprinting.	to study the strain variability among vibrio alginolyticus isolates from different sources by insertion sequence-targeted pcr fingerprinting and whole cell protein profile analysis.	2005	15836741
effects of dopamine on the immunity of white shrimp litopenaeus vannamei.	the total haemocyte count (thc), phenoloxidase activity, respiratory burst, superoxide dismutase (sod) activity, phagocytic activity and clearance efficiency in response to pathogen vibrio alginolyticus were measured when the white shrimp litopenaeus vannamei (20.0+/-1.5 g) were injected individually with dopamine at 10(-8), 10(-7) and 10(-6)mol shrimp(-1), respectively. for the shrimp that received dopamine at 10(-7) and 10(-6)mol shrimp(-1), the thc decreased by 25% and 39%, phenoloxidase acti ...	2005	15863017
interactions of motx with moty and with the poma/pomb sodium ion channel complex of the vibrio alginolyticus polar flagellum.	rotation of the sodium ion-driven polar flagellum of vibrio alginolyticus requires the inner membrane sodium ion channel complex poma/pomb and the outer membrane components motx and moty. none of the detergents used in this study were able to solubilize motx when it was expressed alone. however, when co-expressed with moty, motx was solubilized by some detergents. the change in the solubility of motx suggests that moty interacts with motx. in agreement with this, a pull-down assay showed the ass ...	2005	15866878
the occurrence of vibrio species in tropical shrimp culture environments; implications for food safety.	the occurrence of various vibrio species in water, sediment and shrimp samples from multiple shrimp farm environments from the east and west coast of india was studied. the relative abundance was higher in west coast farms (ca. 10(4) cfu/ml water) when compared to the east coast (ca. 10(2) cfu/ml water). vibrio alginolyticus (3-19%), v. parahaemolyticus (2-13%), v. harveyi (1-7%) and v. vulnificus (1-4%) were the predominant vibrio species identified by standard biochemical testing. in some case ...	2005	15992615
proteomic analysis on the expression of outer membrane proteins of vibrio alginolyticus at different sodium concentrations.	the ability of osmoregulation is crucial to marine pathogens that always face the change of osmotic pressure when they shift between natural marine water-bodies and hosts. previous studies indicated that the expressional patterns of outer membrane proteins (omps) changed when gram-negative bacteria were transferred in different environments. in the present study, proteomic methodologies were used to investigate the expressional pattern of omps of vibrio alginolyticus, a universal marine pathogen ...	2005	16021606
vibrio splendidus biotype 1 as a cause of mortalities in hatchery-reared larval turbot, scophthalmus maximus (l.).	to characterize bacteria associated with turbot larvae feeding on artemia and identify pathogens causing mortalities in larvae.	2005	16033454
investigation of seven vibrio virulence genes among vibrio alginolyticus and vibrio parahaemolyticus strains from the coastal mariculture systems in guangdong, china.	to investigate the distribution of the virulence of two vibrio species among different strains obtained from the mariculture systems on the coast of guangdong in china and the correlation between the virulence strains and the virulence genes among vibrio alginolyticus.	2005	16033522
assembly of motor proteins, poma and pomb, in the na+-driven stator of the flagellar motor.	poma and pomb are transmembrane proteins that form the stator complex in the sodium-driven flagellar motor of vibrio alginolyticus and are believed to surround the rotor part of the flagellar motor. we constructed and observed green fluorescent protein (gfp) fusions of the stator proteins poma and pomb in living cells to clarify how stator proteins are assembled and installed into the flagellar motor. we were able to demonstrate that gfp-poma and gfp-pomb localized to a cell pole dependent on th ...	2005	16038931
white spot syndrome virus isolates of tiger shrimp penaeus monodon (fabricious) in india are similar to exotic isolates as revealed by polymerase chain reaction and electron microscopy.	microbiological analysis of samples collected from cases of white spot disease outbreaks in cultured shrimp in different farms located in three regions along east coast of india viz. chidambram (tamil nadu), nellore (andhra pradesh) and balasore (orissa), revealed presence of vibrio alginolyticus, vibrio parahaemolyticus, and aeromonas spp. but experimental infection trials in penaeus monodon with these isolates did not induce any acute mortality or formation of white spots on carapace. infectio ...	2005	16053274
influence of water temperature and salinity on seasonal occurrences of vibrio cholerae and enteric bacteria in oyster-producing areas of veracruz, méxico.	the influence of temperature and salinity on the occurrence of vibrio cholerae, escherichia coli and salmonella spp. associated with water and oyster samples was investigated in two lagoons on the atlantic coast of veracruz, mexico over a 1-year period. the results indicated that seasonal salinity variability and warm temperatures, as well as nutrient influx, may influence the occurrence of v. cholera. non-o1 and o1. the conditions found in the alvarado (31.12 degrees c, 6.27 per thousand, ph=8. ...	2005	16061261
antibiotic resistance in aeromonas hydrophila and vibrio alginolyticus isolated from a wound infection: a case report.		2005	15674175
the peroxinectin of white shrimp litopenaeus vannamei is synthesised in the semi-granular and granular cells, and its transcription is up-regulated with vibrio alginolyticus infection.	peroxinectin mrna expression in the different types of haemocytes of white shrimp litopenaeus vannamei was studied by in situ hybridisation using digoxigenin-utp-labelled riboprobes and reverse transcription-polymerase chain reaction (rt-pcr). granular cells (gc) and a mixture of semi-granular cells (sgc) and hyaline cells (hc) were separated by 70% percoll gradient centrifugation. peroxinectin was synthesised in both gc and the mixture of sgc-hc. an in situ hybridisation assay indicated that pe ...	2005	15683919
a collagenase-targeted multiplex pcr assay for identification of vibrio alginolyticus, vibrio cholerae, and vibrio parahaemolyticus.	a multiplex pcr assay using three collagenase-targeted primer pairs for the species-specific detection of vibrio alginolyticus, vibrio cholerae, and vibrio parahaemolyticus was developed. the results highlight the species specificity of the three primer sets designed. because of the increasing importance of vibrio spp. in human foodborne diseases, molecular approaches for routine microbial screening and monitoring of clinical, environmental, and food samples also have become more important. the ...	2005	15690817
difference in bacterial motion between forward and backward swimming caused by the wall effect.	a bacterial cell that has a single polar flagellum alternately repeats forward swimming, in which the flagellum pushes the cell body, and backward swimming, in which the flagellum pulls the cell body. we have reported that the backward swimming speeds of vibrio alginolyticus are on average greater than the forward swimming speeds. in this study, we quantitatively measured the shape of the trajectory as well as the swimming speed. the trajectory shape in the forward mode was almost straight, wher ...	2005	15695638
asymmetric swimming pattern of vibrio alginolyticus cells with single polar flagella.	the swimming pattern of bacteria with single polar flagella has usually been described as "run and reverse". we observed the swimming traces of monotrichously flagellated vibrio alginolyticus cells and examined the relationship between the swimming pattern and the sense of progress. swimming in regions other than a solid surface was confirmed to be linear run and reverse. near a solid surface, the traces consisted of "run and arc"; the cells were found to curve sharply during backward swimming, ...	2005	15621441
[cloning and sequence analysis of variable region gene of anti-idiotype monoclonal antibody against vibrio alginolyticus].	to clone and sequence v(h) and v(l) genes of anti-idiotype monoclonal antibody (mab) against vibrio alginolyticus.	2005	15629085
deletion analysis of the carboxyl-terminal region of the pomb component of the vibrio alginolyticus polar flagellar motor.	the stator of the sodium-driven flagellar motor of vibrio alginolyticus is a membrane protein complex composed of four poma and two pomb subunits. pomb has a peptidoglycan-binding motif in the c-terminal region. in this study, four kinds of pomb deletions in the c terminus were constructed. none of the deletion proteins restored motility of the deltapomb strain. the poma protein was coisolated with all of the pomb derivatives under detergent-solubilized conditions. homotypic disulfide cross-link ...	2005	15629950
isolation of vibrio alginolyticus and vibrio splendidus from aquacultured carpet shell clam (ruditapes decussatus) larvae associated with mass mortalities.	two episodes of mortality of cultured carpet shell clams (ruditapes decussatus) associated with bacterial infections were recorded during 2001 and 2002 in a commercial hatchery located in spain. vibrio alginolyticus was isolated as the primary organism from moribund clam larvae that were obtained during the two separate events. vibrio splendidus biovar ii, in addition to v. alginolyticus, was isolated as a result of a mixed vibrio infection from moribund clam larvae obtained from the second mort ...	2005	15640176