Publications

sanitary and bacteriological aspects of sewage treatment.	a study into the removal of contamination load and indicator bacteria was carried out in 1992-1996 in the mechanical, biological and chemical waste-water treatment plant wtp in lezany, in the county of reszel, in the province of warmia and mazury in poland. the results of chemical analyses found a high efficiency of removal of carbon compounds, cod (90%) and bod (98%), in the process of purification of household sewage. in addition, a high effectiveness of total nitrogen, on average 71%, and uns ...	2003	15058814
uterine microbiology and antimicrobial susceptibility in isolated bacteria from mares with fertility problems.	uterine microbiology and antimicrobial susceptibility was investigated in 239 mares with fertility problems in a prospective study in sweden. uterine swab samples were collected with double guarded swabs and transported overnight before being cultured. the minimum inhibitory concentrations (mic) was determined for a panel of antimicrobials. from 152 of the 239 mares at least one bacterial species was isolated, most frequently e. coli (104 isolates), beta-haemolytic streptococci (31) and fungi (1 ...	2003	15074625
variation between pathogenic serovars within salmonella pathogenicity islands.	although four of the five salmonella pathogenicity islands (spis) have been characterized in detail for salmonella enterica serovar typhimurium, and the fifth has been characterized for salmonella enterica serovar dublin, there have been limited studies to examine them in detail in a range of pathogenic serovars of s. enterica. the aim of this study was to examine these regions, shown to be crucial in virulence, in pathogenic serovars to identify any major deletions or insertions that may explai ...	2003	12775700
influence of food preservation parameters and associated microbiota on production rate, profile and stability of acylated homoserine lactones from food-derived enterobacteriaceae.	quorum-dependent regulation is mediated by n-acyl-l-homoserine lactones (ahls) in several gram-negative bacteria. the production of ahls has typically been studied using pure bacteria cultures grown in nutrient-rich media at optimal temperature. ahls are produced in several chill-stored foods by gram-negative bacteria participating in spoilage. as part of our investigation of the role of ahls in food quality, we studied the ahl production in two enterobacteriaceae isolated from cold-smoked salmo ...	2003	12781938
biological control of monilinia laxa and rhizopus stolonifer in postharvest of stone fruit by pantoea agglomerans eps125 and putative mechanisms of antagonism.	treatment of stone fruits (apricot, peach and nectarine) with pantoea agglomerans strain eps125 decreased the incidence and diameter of lesions of brown rot caused by monilinia laxa and soft rot caused by rhizopus stolonifer. root control was achieved on fruits either wounded and subsequently inoculated with the pathogens or non-wounded and naturally infected from orchards. the efficacy of biocontrol was dependent on the concentration of the biocontrol agent and pathogen. at medium to low pathog ...	2003	12781959
a handheld real time thermal cycler for bacterial pathogen detection.	the handheld advanced nucleic acid analyzer (hanaa) is a portable real time thermal cycler unit that weighs under 1 kg and uses silicon and platinum-based thermalcycler units to conduct rapid heating and cooling of plastic reaction tubes. two light emitting diodes (led) provide greater than 1 mw of electrical power at wavelengths of 490 nm (blue) and 525 nm (green), allowing detection of the dyes fam and joe/tamra. results are displayed in real time as bar graphs, and up to three, 4-sample assay ...	2003	12788554
growth of hyperthermophilic archaeon pyrococcus furiosus on chitin involves two family 18 chitinases.	pyrococcus furiosus was found to grow on chitin, adding this polysacharide to the inventory of carbohydrates utilized by this hyperthermophilic archaeon. accordingly, two open reading frames (chia [pf1234] and chib [pf1233]) were identified in the genome of p. furiosus, which encodes chitinases with sequence similarity to proteins from the glycosyl hydrolase family 18 in less-thermophilic organisms. both enzymes contain multiple domains that consist of at least one binding domain and one catalyt ...	2003	12788706
combining culture-dependent and -independent methodologies for estimation of richness of estuarine bacterioplankton consuming riverine dissolved organic matter.	three different methods for analyzing natural microbial community diversity were combined to maximize an estimate of the richness of bacterioplankton catabolizing riverine dissolved organic matter (rdom). we also evaluated the ability of culture-dependent quantitative dna-dna hybridization, a 16s rrna gene clone library, and denaturing gradient gel electrophoresis (dgge) to detect bacterial taxa in the same sample. forty-two different cultivatable strains were isolated from rich and poor solid m ...	2003	12788769
soft tissue infection caused by a novel pigmented, rapidly growing mycobacterium species.	molecular techniques are playing an important role in the diagnosis of nontuberculous mycobacterial infections. this case report describes a chronic soft tissue infection in an immunocompetent patient caused by a previously undescribed pigmented, rapidly growing mycobacterium species, emphasizing the importance of clinical suspicion and effective laboratory techniques in the diagnosis and treatment of infection.	2003	12791930
survey of plasmid-associated genetic markers in enterobacteriaceae with reduced susceptibilities to cephalosporins.	clinical isolates of enterobacteriaceae with reduced susceptibilities to cephalosporins were collected from 1993 to 2000. the organisms were screened for the extended-spectrum beta-lactamase (esbl) phenotype, and plasmid extracts were screened for genetic markers by hybridization. a bla(tem) probe was derived from puc19; other probes were derived from pacm1, the plasmid responsible for the first known appearance of an esbl in our institution. these probes included bla(shv), int, aac(3)-ia, dfra1 ...	2003	12821465
the emulsiv filter removes microbial contamination from propofol but is not a substitute for aseptic technique.	to evaluate the ability of the emulsiv filter (ef) to remove extrinsic microbial contaminants from propofol.	2003	12826543
structural and functional analysis of pantocin a: an antibiotic from pantoea agglomerans discovered by heterologous expression of cloned genes.		2003	12833353
detection and diversity assessment of xylella fastidiosa in field-collected plant and insect samples by using 16s rrna and gyrb sequences.	the causal agent of diseases in many economically important plants is attributed to the xylem-limited bacterium xylella fastidiosa. the detection of this plant pathogen has been hampered due to its difficult isolation and slow growth on plates. nearly complete nucleotide sequences of the 16s rrna gene and partial sequences of the gyrb gene were determined for 18 strains of x. fastidiosa isolated from different plant hosts. a phylogenetic analysis, based on gyrb, grouped strains in three clusters ...	2003	12839807
structure of dried cellular alginate matrix containing fillers provides extra protection for microorganisms against uvc radiation.	soil microorganisms in general and biocontrol agents in particular are very sensitive to uv light. the packaging of biocontrol microorganisms into cellular solids has been developed as a means of reducing loss caused by exposure to environmental uv radiation. the bacterial and fungal biocontrol agents pantoea agglomerans and trichoderma harzianum were immobilized in freeze-dried alginate beads containing fillers and subjected to 254 nm uv radiation (uvc). immobilization of cells in freeze-dried ...	2003	12859230
extended-spectrum beta-lactamases in ireland, including a novel enzyme, tem-102.	organisms producing extended-spectrum beta-lactamases (esbls) have been reported in many countries, but there is no information on the prevalence of esbl-producing members of the family enterobacteriaceae in ireland. a total of 925 isolates of ampicillin-resistant members of the enterobacteriaceae were received from six hospitals in ireland over a 3-year period from september 1996 to september 1999. isolates were screened for esbl production by the double-disk diffusion (ddd) method. ddd-positiv ...	2003	12878521
a comparison of the bacteria found on the hands of 'homemakers' and neonatal intensive care unit nurses.	this prevalence study was conducted to compare the counts, types and antimicrobial resistance profiles of bacterial flora on the hands of individuals in the community to that of nurses at a nearby university teaching hospital, with an intense hand hygiene regimen. hand cultures were obtained from 204 individuals during a home visit and 119 nurses in two neonatal intensive care units (nicus). the mean total log counts of organisms were 5.73 and 5.24 for the homemakers [defined as the person (usua ...	2003	12919763
in vitro activities of abt-492, a new fluoroquinolone, against 155 aerobic and 171 anaerobic pathogens isolated from antral sinus puncture specimens from patients with sinusitis.	abt-492 exhibited excellent in vitro activities against all 326 aerobic and anaerobic antral puncture sinus isolates tested with mics (in micrograms per milliliter) at which 90% of the isolates tested were inhibited as follows: haemophilus influenzae, 0.001; moraxella catarrhalis, 0.008; and streptococcus pneumoniae, 0.015. it was four- to sixfold more active than other fluoroquinolones, including against levofloxacin-resistant strains of s. pneumoniae, methicillin-resistant staphylococcus aureu ...	2003	12937015
in vivo reconstitution of the fhua transport protein of escherichia coli k-12.	the fhua protein in the outer membrane of escherichia coli actively transports ferrichrome and the antibiotics albomycin and rifamycin cgp 4832 and serves as a receptor for the phages t1, t5, and phi80 and for colicin m and microcin j25. the crystal structure reveals a beta-barrel with a globular domain, the cork, which closes the channel formed by the barrel. genetic deletion of the cork resulted in a beta-barrel that displays no fhua activity. a functional fhua was obtained by cosynthesis of s ...	2003	12949103
optical properties of erwinia herbicola bacteria at 0.190-2.50 microm.	we measure the complex index of refraction of erwina herbicola (also known as enterobacter agglomerans or pantoea agglomerans) bacteria (attc 33243) over the spectral region from 0.190 to 2.50 microm (4000-52,632 cm(-1)). transmission measurements are made on solid films of e. herbicola and on suspensions of the bacteria in water. these measurements, combined with spectral reflectance and kramers-krönig analysis, allow the determination of the real and imaginary parts over the entire wavelength ...	2003	12949829
morphological, host range, and genetic characterization of two coliphages.	two coliphages, ar1 and lg1, were characterized based on their morphological, host range, and genetic properties. transmission electron microscopy showed that both phages belonged to the myoviridae; phage particles of lg1 were smaller than those of ar1 and had an isometric head 68 nm in diameter and a complex contractile tail 111 nm in length. transmission electron micrographs of ar1 showed phage particles consisting of an elongated isometric head of 103 by 74 nm and a complex contractile tail 1 ...	2003	12957924
the influence of antibiotic production and pre-emptive colonization on the population dynamics of pantoea agglomerans (erwinia herbicola) eh1087 and erwinia amylovora in planta.	stigma colonization by erwinia amylovora is the crucial first step in the development of most fire blight infections in apple and pear trees. suppression at this point of the disease process by antagonists of e. amylovora, such as pantoea agglomerans (erwinia herbicola) strain eh1087, is a rational approach to control fire blight. we tested the hypothesis that the ability of e. amylovora to compete with eh1087 for colonization of a stigma is reduced by the potential for eh1087 to produce the phe ...	2003	14510856
frameshift mutation events in beta-glucosidases.	compensated frameshift mutation is a modification of the reading frame of a gene that takes place by way of various molecular events. it appears to be a widespread event that is only observed when homologous amino acid and nucleodotide sequences are compared. to identify these mutation events, the sequence analysis rationale was based on the search for short regions that would have much lower degrees of conservation in protein, but not in dna, in well-conserved beta-glucosidase families. we have ...	2003	14527732
recovery of surface bacteria from and surface sanitization of cantaloupes.	practical, effective methods that could be implemented in a food service establishment (restaurant or delicatessen) for the surface sanitization of cantaloupes were microbiologically evaluated. cantaloupes (cucumis melo l. var. reticulates) were immersed in an inoculum containing salmonella enterica serovar poona or pantoea agglomerans at ca. 10(4) to 10(5) cfu/ml. an efficient method for the recovery of bacteria from the cantaloupe surface was developed and validated. the method consisted of wa ...	2003	14572217
microbial diversity and prevalence of virulent pathogens in biofilms developed in a water reclamation system.	bacterial biofilm is a common phenomenon in both natural and engineered systems which often becomes a source of contamination and microbially influenced corrosion. it is thought that formation of biofilm in the monoculture of several bacterial species is regulated by acylhomoserine lactone (ahl) quorum-sensing signals. in this study, we investigated the microbial diversity and existence of ahl-producing and ahl-degrading bacterial species in the biofilm samples from a water reclamation system lo ...	2003	14596899
low-temperature isolation of disease-suppressive bacteria and characterization of a distinctive group of pseudomonads.	the influence of environmental factors during isolation on the composition of potential biocontrol isolates is largely unknown. bacterial isolates that efficiently suppressed wheat seedling blight caused by fusarium culmorum were found by isolating psychrotrophic, root-associated bacteria and by screening them in a bioassay that mimicked field conditions. the impact of individual isolation factors on the disease-suppressive index (dsi) of almost 600 isolates was analyzed. the bacteria originated ...	2003	14602601
role of receptor proteins for enterobactin and 2,3-dihydroxybenzoylserine in virulence of salmonella enterica.	single, double, and triple mutants of an enterobactin-deficient mutant strain of salmonella enterica serovar typhimurium were constructed that were defective in the expression of the iron-regulated outer membrane proteins (iromps) fepa, iron, and cir, which are proposed to function as catecholate receptors. uptake of naturally occurring and chemically synthesized catecholate molecules by these mutants was assessed in standard growth promotion assays. unique patterns of uptake were identified for ...	2003	14638784
enumeration and characterization of iron(iii)-reducing microbial communities from acidic subsurface sediments contaminated with uranium(vi).	iron(iii)-reducing bacteria have been demonstrated to rapidly catalyze the reduction and immobilization of uranium(vi) from contaminated subsurface sediments. thus, these organisms may aid in the development of bioremediation strategies for uranium contamination, which is prevalent in acidic subsurface sediments at u.s. government facilities. iron(iii)-reducing enrichment cultures were initiated from pristine and contaminated (high in uranium, nitrate; low ph) subsurface sediments at ph 7 and ph ...	2003	14660400
single-copy green fluorescent protein gene fusions allow accurate measurement of salmonella gene expression in vitro and during infection of mammalian cells.	we developed a reliable and flexible green fluorescent protein (gfp)-based system for measuring gene expression in individual bacterial cells. until now, most systems have relied upon plasmid-borne gfp gene fusions, risking problems associated with plasmid instability. we show that a recently developed gfp variant, gfp+, is suitable for assessing bacterial gene expression. various gfp+ transcriptional fusions were constructed and integrated as single copies into the chromosome of salmonella ente ...	2003	14660401
genetic characterization of the carotenoid biosynthetic pathway in methylobacterium extorquens am1 and isolation of a colorless mutant.	genomic searches were used to reconstruct the putative carotenoid biosynthesis pathway in the pink-pigmented facultative methylotroph methylobacterium extorquens am1. four genes for putative phytoene desaturases were identified. a colorless mutant was obtained by transposon mutagenesis, and the insertion was shown to be in one of the putative phytoene desaturase genes. mutations in the other three did not affect color. the tetracycline marker was removed from the original transposon mutant, resu ...	2003	14660416
a double application approach to ophthalmia neonatorum prophylaxis.	to investigate if a second drop of 2.5% povidone-iodine ophthalmic solution placed within the first postnatal day would achieve better prophylaxis against ophthalmia neonatorum than a single drop applied at birth.	2003	14660449
fear of bioterrorism and implications for public health preparedness.	after the human anthrax cases and exposures in 2001, the illinois department of public health received an increasing number of environmental and human samples (1,496 environmental submissions, all negative for bacillus anthracis). these data demonstrate increased volume of submissions to a public health laboratory resulting from fear of bioterrorism.	2003	12702237
purification and partial characterization of azoreductase from enterobacter agglomerans.	azoreductase, an enzyme catalyzing the reductive cleavage of the azo bond of methyl red (mr) and related dyes, was purified to electrophoretic homogeneity from enterobacter agglomerans. this bacterial strain, isolated from dye-contaminated sludge, has a higher ability to grow, under aerobic conditions, on culture medium containing 100mg/l of mr. the enzyme was purified approximately 90-fold with 20% yield by ammonium sulfate precipitation, followed by three steps of column chromatography (gel-fi ...	2003	12706351
characterization of two tetrachloroethene-reducing, acetate-oxidizing anaerobic bacteria and their description as desulfuromonas michiganensis sp. nov.	two tetrachlorethene (pce)-dechlorinating populations, designated strains bb1 and brs1, were isolated from pristine river sediment and chloroethene-contaminated aquifer material, respectively. pce-to-cis-1,2-dichloroethene-dechlorinating activity could be transferred in defined basal salts medium with acetate as the electron donor and pce as the electron acceptor. taxonomic analysis based on 16s rrna gene sequencing placed both isolates within the desulfuromonas cluster in the delta subdivision ...	2003	12732573
evaluation of the vitek 2 id-gnb assay for identification of members of the family enterobacteriaceae and other nonenteric gram-negative bacilli and comparison with the vitek gni+ card.	we evaluated the vitek 2 id-gnb identification card (biomérieux, inc., durham, n.c.) for its ability to identify members of the family enterobacteriaceae and other gram-negative bacilli that are isolated in clinical microbiology laboratories. using 482 enteric stock cultures and 103 strains of oxidase-positive, gram-negative glucose-fermenting and nonfermenting bacilli that were maintained at -70 degrees c and passaged three times before use, we inoculated cards according to the manufacturer's d ...	2003	12734254
in vitro activities of telithromycin and 10 oral agents against aerobic and anaerobic pathogens isolated from antral puncture specimens from patients with sinusitis.	a study of the comparative in vitro activity of telithromycin, a new ketolide, against 155 aerobic and 171 anaerobic antral sinus puncture isolates showed it to be active against a broad range of sinus pathogens. all pneumococci, including erythromycin-resistant strains, were susceptible to telithromycin at < or = 0.5 microg/ml; all haemophilus influenzae and eikenella corrodens strains were inhibited by < or = 4 microg of telithromycin/ml; all moraxella spp. and beta-lactamase-producing prevote ...	2003	12760875
pantoea agglomerans as a cause of septic arthritis after palm tree thorn injury; case report and literature review.	we report the case of a 14 year old healthy boy, who was admitted six weeks after being injured by a palm tree thorn, with limping caused by pain and swelling in his right knee. an ultrasound examination revealed a foreign body in the posterior lateral aspect of the right knee. pantoea agglomerans was identified in the synovial fluid. the patient underwent two arthrotomies and was treated with amoxicillin-clavulanate intravenously for three weeks. the postoperative course was uneventful, and joi ...	2003	12765929
health effects of inhalation exposure to organic dust in hops farmers.	medical examinations were performed in a group of 23 hops farmers exposed to organic dust from hop (humulus lupulus). the examinations took place in individual farms during harvesting, sorting and transporting of hop cones. as a reference group, 50 urban dwellers not exposed to organic dust were examined. there were conducted physical examinations, interviews concerning the occurrence of respiratory disorders and work-related symptoms, lung function tests, determination of cytokines concentratio ...	2003	15315032
pantoea agglomerans pvs. gypsophilae and betae, recently evolved pathogens?	summary pantoea agglomerans pvs. gypsophilae and betae taxonomy: bacteria; proteobacteria; gamma subdivision; order enterobacteriales; family enterobacteriaceae; species pantoea agglomerans. microbiological properties: gram-negative, non-capsulated, non-spore-forming, predominately motile rode. disease symptoms: gall formation at wound sites, mainly in the crown region of the stem. the host range of p. agglomerans pv. gypsophilae is restricted to gypsophila paniculata, whereas p. agglomerans pv. ...	2003	20569391
versatility of aminoglycosides and prospects for their future.	aminoglycoside antibiotics have had a major impact on our ability to treat bacterial infections for the past half century. whereas the interest in these versatile antibiotics continues to be high, their clinical utility has been compromised by widespread instances of resistance. the multitude of mechanisms of resistance is disconcerting but also illuminates how nature can manifest resistance when bacteria are confronted by antibiotics. this article reviews the most recent knowledge about the mec ...	2003	12857776
comparing the dehalogenase gene pool in cultivated alpha-halocarboxylic acid-degrading bacteria with the environmental metagene pool.	culture-dependent and culture-independent approaches were used to determine the relationship between the dehalogenase gene pool in bacteria enriched and isolated on 2,2-dichloropropionic acid (22dcpa) and the environmental metagene pool (the collective gene pool of both the culturable and uncultured microbes) from which they were isolated. the dehalogenases in the pure-cultures isolates, which were able to degrade 22dcpa, were similar to previously described group i and ii dehalogenases. signifi ...	2003	12902218
synergistic antimicrobial activity of metabolites produced by a nonobligate bacterial predator.	a naturally occurring, gram-negative, nonobligate predator bacterial strain 679-2, exhibits broad-spectrum antimicrobial activity that is due, in part, to the production of three extracellular compounds. antimicrobial-activity-directed fractionation of a culture of strain 679-2 against a panel of microorganisms has led to the isolation of three compounds: pyrrolnitrin, maculosin, and a new compound, which we have named banegasine. although pyrrolnitrin is well known in the literature, it has not ...	2003	12821455
microbiology of the phyllosphere.		2003	12676659
epidemiologic background of hand hygiene and evaluation of the most important agents for scrubs and rubs.	the etiology of nosocomial infections, the frequency of contaminated hands with the different nosocomial pathogens, and the role of health care workers' hands during outbreaks suggest that a hand hygiene preparation should at least have activity against bacteria, yeasts, and coated viruses. the importance of efficacy in choosing the right hand hygiene product is reflected in the new centers for disease control and prevention guideline on hand hygiene (j. m. boyce and d. pittet, morb. mortal. wkl ...	2004	15489352
impact of 16s rrna gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases.	the traditional identification of bacteria on the basis of phenotypic characteristics is generally not as accurate as identification based on genotypic methods. comparison of the bacterial 16s rrna gene sequence has emerged as a preferred genetic technique. 16s rrna gene sequence analysis can better identify poorly described, rarely isolated, or phenotypically aberrant strains, can be routinely used for identification of mycobacteria, and can lead to the recognition of novel pathogens and noncul ...	2004	15489351
relationship between triclosan and susceptibilities of bacteria isolated from hands in the community.	the possible association between triclosan and bacterial susceptibility to antibiotic was examined among staphylococci and several species of gram-negative bacteria (gnb) isolated from the hands of individuals in a community setting. hand cultures from individuals randomized to using either antibacterial cleaning and hygiene products (including a hand soap containing 0.2% triclosan) or nonantibacterial cleaning and hygiene products for a 1-year period were taken at baseline and at the end of the ...	2004	15273108
microarray-based identification of bacteria in clinical samples by solid-phase pcr amplification of 23s ribosomal dna sequences.	the rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. we describe a dna microarray-based pcr approach for the quick detection and identification of bacteria from cervical swab specimens from mares. this on-chip pcr method combines the amplification of a variable region of bacterial 23s ribosomal dna and the simultaneous sequence-specific detection on a solid phase. the solid phase contains bacterial species-specific primers co ...	2004	15004052
a class of plant glycosyltransferases involved in cellular homeostasis.	many small lipophilic compounds in living cells can be modified by glycosylation. these processes can regulate the bioactivity of the compounds, their intracellular location and their metabolism. the glycosyltransferases involved in biotransformations of small molecules have been grouped into family 1 of the 69 families that are classified on the basis of substrate recognition and sequence relatedness. in plants, these transfer reactions generally use udp-glucose with acceptors that include horm ...	2004	15241472
one recognition sequence, seven restriction enzymes, five reaction mechanisms.	the diversity of reaction mechanisms employed by type ii restriction enzymes was investigated by analysing the reactions of seven endonucleases at the same dna sequence. nari, kasi, mly113i, sfoi, egei, ehei and bbei cleave dna at several different positions in the sequence 5'-ggcgcc-3'. their reactions on plasmids with one or two copies of this sequence revealed five distinct mechanisms. these differ in terms of the number of sites the enzyme binds, and the number of phosphodiester bonds cleave ...	2004	15226412
comparison of atpase-encoding type iii secretion system hrcn genes in biocontrol fluorescent pseudomonads and in phytopathogenic proteobacteria.	type iii protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. certain type iii protein secretion genes (e.g., hrcc) have been found in pseudomonas sp. strain sbw25 (and other biocontrol pseudomonads), but other type iii protein secretion genes, such as the atpase-encoding gene hrcn, have not been found. using both colony hybridization and a pcr approach, we show here that hrcn is nevertheles ...	2004	15345390
malt sprout extract medium for cultivation of lactobacillus plantarum protective cultures.	the aim was to develop a cheap cereal-based alternative medium for the large-scale production of biopreservative lactobacillus plantarum vtt e-79098. we examined the effect of growth medium and ph control on the cell yield of lact. plantarum e-79098 and the antimicrobial activity of the cell-free extracts.	2004	15355535
genomic dna detection using cycling probe technology and capillary gel electrophoresis with laser-induced fluorescence.	cycling probe technology (cpt) is an isothermal dna analysis method that has been shown to be useful for identifying genetic markers of antibiotic-resistant bacteria in clinical settings. cpt assays have previously employed several assay methods that include polyacrylamide gel electrophoresis and magnetic beads for separations and radioisotopic and colorimetric detection for detection. capillary gel electrophoresis with laser-induced fluorescence (cge-lif) is an alternative separation and detect ...	2004	15356906
vibrio spp. and salmonella spp., presence and susceptibility in crabs ucides cordatus.	the presence of vibrio spp. and salmonella spp. in crabs marketed at the bezerra de menezes ave., fortaleza, state of ceará, brazil, was assessed between february and may, 2003. the number of individuals sampled in each one of the fifteen weekly samplings ranged between four and eight. seven strains of salmonella, from four different samplings, were identified, being five of them identified as serotype s. senftenberg and two as s. poona. all strains of salmonella were sensitive to the tested ant ...	2004	15361967
bacteremic infection with pantoea ananatis.	a 73-year-old man was hospitalized for dyspnea and bilateral ankle edema. during his hospital stay he presented anal hemorrhage and developed a high fever after colonoscopy. a set of aerobic and anaerobic blood culture bottles yielded a pure culture of gram-negative rods, susceptible to all antibiotics tested. the api20e code was 1005133, resulting in a very good identification as pantoea sp. subsequent sequencing of the 16s rrna gene revealed a final identification as pantoea ananatis. the pati ...	2004	15365053
gamma proteobacteria can nodulate legumes of the genus hedysarum.	the bacteria hosted in the root nodules of the three mediterranean wild legume species hedysarum carnosum, hedysarum spinosissimum subsp. capitatum, and hedysarum pallidum, growing in native stands in different habitats in algeria were isolated. bacteria were recovered on yeast-mannitol-agar or on minimal media from a total of 52 nodules. isolates were analyzed by amplified ribosomal dna restriction analysis (ardra) using the enzyme cfoi, and further sorted by rapd fingerprinting. a total of ten ...	2004	15368852
improved laboratory diagnosis of bacterial and fungal infections in patients with hematological malignancies using pcr and ribosomal rna sequence analysis.	during october 1999 to november 2000, 98 blood culture specimens from the same number of febrile episodes originating from 49 patients with hematological malignancies were examined for the presence of eubacteria and fungi based on 16s rrna gene and the 5.8, 18 and 28s rrna combined with in vitro pcr amplification and sequencing, in addition to conventional blood culture laboratory techniques. nineteen of the samples were associated with positive blood cultures. eubacterial (16s rrna) pcr detecte ...	2004	15370218
bacterial overgrowth in the cystic fibrosis transmembrane conductance regulator null mouse small intestine.	we recently reported the inflammation of the cystic fibrosis (cf) mouse small intestine, and we hypothesized bacterial overgrowth as a possible cause. quantitative pcr of bacterial 16s genomic dna in the cf mouse small intestine revealed an increase of greater than 40-fold compared to controls. sequencing of 16s pcr products and gram staining showed that the majority of bacteria in the cf mouse intestine were gram negative. bacteria were observed to colonize the mucus that accumulates in the int ...	2004	15385508
[multiple change of phenotype, conjugated with the loss of yellow pigmentation of erwinia herbicola].	it has been shown that the loss of yellow pigmentation (phenotype crt) of nonphotosynthesizing epiphyte bacterium erwinia herbicola is accompanied by the loss of prototrophicity (phenotype thi). most crt thi-variants change the character of sensitivity to temperate erwiniophage e105 and bacteriocins (phenotype ph/bn). some of them become sensitive to the killer effect of their own bacteriocins--autocins (phenotype au). multiple change of the phenotype in e. herbicola occurs so spontaneously as u ...	2004	15456215
rhizosheath of sinai desert plants is a potential repository for associative diazotrophs.	among 42 plant species representing the flora of north sinai, two possessed sand grain sheath encasing the roots. they are panicum turgidum forssk. and stipagrostis scoparia (trin.and rupr.) dewinter. rhizosheaths, compared to surrounding free sand, accommodated higher population density of microorganisms including associative diazotrophs. isolates secured belonged to the species of bacillus circulans, paenib. macerans (bacillus macerans), enterobacter agglomerans, agrobacterium radiobacter and ...	2004	15462528
cloning and expression of a phloretin hydrolase gene from eubacterium ramulus and characterization of the recombinant enzyme.	phloretin hydrolase catalyzes the hydrolytic c-c cleavage of phloretin to phloroglucinol and 3-(4-hydroxyphenyl)propionic acid during flavonoid degradation in eubacterium ramulus. the gene encoding the enzyme was cloned by screening a gene library for hydrolase activity. the insert of a clone conferring phloretin hydrolase activity was sequenced. sequence analysis revealed an open reading frame of 822 bp (phy), a putative promoter region, and a terminating stem-loop structure. the deduced amino ...	2004	15466559
[effects of splenectomy on peritonitis produced by a colonic injury: study in rats].	study the effects of splenectomy on the intra-abdominal infection by bowel flora, consequent to a colonic injury in wistar rats.	2004	15499477
volatiles production and attractiveness to the mexican fruit fly of enterobacter agglomerans isolated from apple maggot and mexican fruit flies.	we investigated two strains of uricase (+) enterobacter agglomerans, one isolated from the apple maggot fly (amf) and one from the mexican fruit fly (mff), for 1) attractiveness to mff, and 2) production of attractive chemicals. regarding chemicals demonstrated attractive to the mff, the mff bacterial strain produced more 2,5-dimethylpyrazine, 2-phenylethanol, and indole than the amf strain, whereas the amf, but not the mff strain, produced 3-hydroxybutanone. cell types that predominated in plat ...	2004	15503523
identification and phylogeny of enterobacter sakazakii relative to enterobacter and citrobacter species.	the phylogenetic relationships of enterobacter sakazakii strains were investigated using 16s ribosomal dna (rdna) and hsp60 sequencing. each analysis distributed e. sakazakii strains among four clusters, indicating substantial taxonomic heterogeneity. the e. sakazakii type strain 16s rdna sequence was 97.8% similar to that of citrobacter koseri but 97.0% similar to that of enterobacter cloacae.	2004	15528745
intestinal microflora in 45 crows in ueno zoo and the in vitro susceptibilities of 29 escherichia coli isolates to 14 antimicrobial agents.	microorganisms from 45 jungle crows (corvus macrorhynchos) captured from july to december 2002 at ueno zoo, tokyo were identified as escherichia coli, proteus mirabilis, klebsiella pneumoniae, klebsiella oxytoca, enterobacter aerogenes, enterobacter cloacae, enterobacter agglomerans, pseudomonas maltophila, staphylococcus spp., micrococcus spp., and streptococcus spp. e. coli showed the highest rate of isolation (21.6%). in an in vitro susceptibility test for 29 isolates of e. coli to 14 antimic ...	2004	15528866
proposal to acknowledge beijerinck as the original author of the species pantoea agglomerans. request for an opinion.	the name 'bacillus agglomerans' was first published by beijerinck in 1888. ewing and fife changed the name to enterobacter agglomerans in 1972 as a new combination, acknowledging that beijerinck had been the original author and making the new full name enterobacter agglomerans (beijerinck 1888) ewing and fife 1972. beijerinck's name was omitted from the approved lists of bacterial names in 1980, which listed only ewing and fife as the authors. the current listings in the list of bacterial names ...	2004	15545493
analysis in vitro of the enzyme crtiso establishes a poly-cis-carotenoid biosynthesis pathway in plants.	most enzymes in the central pathway of carotenoid biosynthesis in plants have been identified and studied at the molecular level. however, the specificity and role of cis-trans-isomerization of carotenoids, which occurs in vivo during carotene biosynthesis, remained unresolved. we have previously cloned from tomato (solanum lycopersicum) the crtiso gene, which encodes a carotene cis-trans-isomerase. to study the biochemical properties of the enzyme, we developed an enzymatic in vitro assay in wh ...	2004	15557094
cloning, sequencing and partial characterisation of sorbitol transporter (srlt) gene encoding phosphotransferase system, glucitol/sorbitol-specific iibc components of erwinia herbicola atcc 21998.	a dna fragment of approximately 1500 bp, harbouring the sorbitol transport gene (srlt), was amplified from the chromosomal dna of erwinia herbicola atcc 21998 by pcr and cloned in escherichia coli jm109. degenerate oligonucleotide primers used were designed based on the conserved regions in the gene sequences within the gut operon of e. coli (gene bank accession no. j02708) and the srl operon of erwinia amylovora (gene bank accession no. y14603). the cloned dna fragment was sequenced and found t ...	2004	15560368
effect of probiotic adult diets on fitness components of sterile male mediterranean fruit flies (diptera: tephritidae) under laboratory and field cage conditions.	the aim of the current study was to investigate the effect of probiotic adult diets, i.e., adult diets containing viable symbiotic intestinal bacteria, on the pheromone-calling activity, mating success, life expectancy, and survival of mass-reared male mediterranean fruit flies, ceratitis capitata (wiedemann), as an avenue for improving the field performance of sterile males in release programs to eradicate, suppress, or prevent spread of wild populations. the effect of inoculation of two standa ...	2004	15568345
diagnostic real-time pcr for detection of salmonella in food.	a robust 5' nuclease (taqman) real-time pcr was developed and validated in-house for the specific detection of salmonella in food. the assay used specifically designed primers and a probe target within the ttrrsbca locus, which is located near the salmonella pathogenicity island 2 at centisome 30.5. it is required for tetrathionate respiration in salmonella. the assay correctly identified all 110 salmonella strains and 87 non-salmonella strains tested. an internal amplification control, which is ...	2004	15574899
exposure to airborne microorganisms, dust and endotoxin during flax scutching on farms.	microbiological air sampling was performed on 5 flax farms located in eastern poland. air samples for determination of the concentrations of microorganisms, dust and endotoxin were collected in barns during machine scutching of flax stems by the farmers. the concentrations of mesophilic bacteria ranged from 203.5-698.8 x 10(3) cfu/m3, of gram-negative bacteria from 27.2-123.4 x 10(3) cfu/m3, of thermophilic actinomycetes from 0.5-2.6 x 10(3) cfu/m3, and of fungi from 23.4-99.8 x 10(3) cfu/m3. th ...	2004	15627342
studies on the occurrence of gram-negative bacteria in ticks: ixodes ricinus as a potential vector of pasteurella.	a total of 372 ixodes ricinus ticks (101 females, 122 males, and 149 nymphs) collected by flagging in 6 mixed woodlands of eastern poland were examined by culture for the presence of internal gram-negative bacteria other than borrelia burgdorferi. adult ticks were examined in pools of 2 specimens each and nymphs were examined in pools of 3-5 specimens each. ticks were disinfected in 70 % ethanol and homogenized in 0.85% nacl. the diluted homogenate was inoculated onto 3 kinds of agar media: buff ...	2004	15627343
a farmer's occupational airborne contact dermatitis masqueraded by coexisting rosacea: delayed diagnosis and legal acknowledgement.	a rare case of coexistence of occupational airborne dermatitis with rosacea is presented in a 41-year-old female farmer. her first dermatitis symptoms appeared at the age of 10 when she started helping her parents on the farm. uncovered skin areas of the face, neck, décolleté, forearms and the hands gradually became involved. the dermatitis symptoms were provoked by agricultural dusts (especially of flax and dried herbs). for the subsequent 30 years, the work-related disease remained undiagnosed ...	2004	15627345
a predictive model for heat inactivation of listeria monocytogenes biofilm on stainless steel.	heat treatment of potential biofilm-forming sites is sometimes used for control of listeria monocytogenes in food processing plants. however, little information is available on the heat treatment required to kill l. monocytogenes present in biofilms. the purpose of this study was to develop a predictive model for the heat inactivation of l. monocytogenes in monoculture biofilms (strains scott a and 3990) and in biofilms with competing bacteria (pseudomonas sp. and pantoea agglomerans) formed on ...	2004	15633676
late infective endocarditis after cholecystectomy in a patient with repaired tetralogy of fallot: a case report.	late endocarditis after surgical repair of tetralogy of fallot is rare. we describe a case of endocarditis following cholecystectomy in a 22-year old patient with repaired tetralogy of fallot. after cholecystectomy, the patient was referred to a cardiology clinic with unexplained fever and suspicion of endocarditis. echocardiography revealed a large mass at the basal level of interventricular septum. endocarditis was diagnosed on the basis of clinical and echocardiographic findings and antibioti ...	2004	15856630
role of glutamine synthetase in phenazine antibiotic production by pantoea agglomerans eh1087.	pantoea agglomerans strain eh1087 produces the phenazine antibiotic d-alanylgriseoluteic acid. a glutamine auxotroph harboring an insertion in a putative glna gene was obtained by transposon-mutagenesis of eh1087 that produced less d-alanylgriseoluteic acid than the parental strain (strain eh7.1). cosmids encoding the eh1087 glna were isolated by their ability to complement the mutant for prototrophy. the role of the eh1087 glna locus was functionally confirmed by complementation of an escherich ...	2004	15644904
purification and properties of a phytate-degrading enzyme from pantoea agglomerans.	a periplasmatic phytate-degrading enzyme from pantoea agglomerans isolated from soil was purified about 470-fold to apparent homogeneity with a recovery of 16% referred to the phytate-degrading activity in the crude extract. it behaved as a monomeric protein with a molecular mass of about 42 kda. the purified enzyme exhibited a single ph optimum at 4.5. optimum temperature for the degradation of phytate was 60 degrees c. the kinetic parameters for the hydrolysis of sodium phytate were determined ...	2004	15648978
degradation of myo-inositol hexakisphosphate by a phytate-degrading enzyme from pantoea agglomerans.	high-pressure liquid chromatography (hplc) analysis established myo-inositol pentakisphosphate as the final product of phytate dephosphorylation by the phytate-degrading enzyme from pantoea agglomerans. neither product inhibition by phosphate nor inactivation of the pantoea enzyme during the incubation period were responsible for the limited phytate hydrolysis as shown by addition of phytate-degrading enzyme and phytate, respectively, after the observed stop of enzymatic phytate degradation. in ...	2004	15648979
an improved method for the enzymatic transformation of nucleosides into 5'-monophosphates.	an improved method to transform nucleosides into 5'-monophosphates using nucleoside phosphotransferase from erwinia herbicola is reported. the method is based on the shift in the equilibrium state of the reaction to the formation of desired product due to its precipitation by zn2+. under optimal conditions, the extent of nucleoside transformations into nucleoside-5'-monophosphates were 41-91% (mol).	2004	15672226
biosorption and recycling of gold using various microorganisms.	in order to obtain basic information on the biosorption and recycling of gold from aqueous systems using microbial cells, the biosorption of gold by various microorganisms was investigated. of 75 strains of microorganisms tested (25 bacteria, 19 actinomycetes, 17 fungi and 14 yeasts), high abilities of gold biosorption from a solution containing hydrogen tetrachloroaurate (iii) were found in some gram-negative bacterial strains, such as acinetobacter calcoaceticus, erwinia herbicola, pseudomonas ...	2004	15754248
bacterial species specificity in prou osmoinducibility and nptii and lacz expression.	reporter gene-based transcriptional fusions are increasingly being used to address questions in microbial ecology, with constitutively expressed fusions enabling microbe tracking and inducible fusions reporting the presence of environmental signals. to more readily apply this technology to a variety of bacterial species, we examined species specificity in the expression of three promoters of interest. a comparison of two potentially constitutive promoters, each fused to the reporter gene gfp, sh ...	2004	16179797
isolation and characterization of an rip (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity.	ribosome-inactivating proteins (rips) are n-glycosidases that remove a specific adenine from the sarcin/ricin loop of the large rrna, thus arresting protein synthesis at the translocation step. in the present study, a protein termed tobacco rip (trip) was isolated from tobacco (nicotiana tabacum) leaves and purified using ion exchange and gel filtration chromatography in combination with yeast ribosome depurination assays. trip has a molecular mass of 26 kd as evidenced by sodium dodecyl sulfate ...	2004	14671015
isolation of endophytic diazotroph pantoea agglomerans and nondiazotroph enterobacter asburiae from sweetpotato stem in japan.	to isolate and identify diazotrophic endophytes in the stem of japanese sweetpotato cv. koganesengan.	2004	14687210
in vitro activities of the novel cephalosporin lb 11058 against multidrug-resistant staphylococci and streptococci.	lb 11058 is a novel parenteral cephalosporin with a c-3 pyrimidinyl-substituted vinyl sulfide group and a c-7 2-amino-5-chloro-1,3-thiazole group. this study evaluated the in vitro activity and spectrum of lb 11058 against 1,245 recent clinical isolates, including a subset of gram-positive strains with specific resistant phenotypes. lb 11058 was very active against streptococcus pneumoniae. the novel cephalosporin was 8- to 16-fold more potent than ceftriaxone, cefepime, or amoxicillin-clavulana ...	2004	14693518
structure of hrcqb-c, a conserved component of the bacterial type iii secretion systems.	type iii secretion systems enable plant and animal bacterial pathogens to deliver virulence proteins into the cytosol of eukaryotic host cells, causing a broad spectrum of diseases including bacteremia, septicemia, typhoid fever, and bubonic plague in mammals, and localized lesions, systemic wilting, and blights in plants. in addition, type iii secretion systems are also required for biogenesis of the bacterial flagellum. the hrcq(b) protein, a component of the secretion apparatus of pseudomonas ...	2004	14694203
structure of hrcqb-c, a conserved component of the bacterial type iii secretion systems.	type iii secretion systems enable plant and animal bacterial pathogens to deliver virulence proteins into the cytosol of eukaryotic host cells, causing a broad spectrum of diseases including bacteremia, septicemia, typhoid fever, and bubonic plague in mammals, and localized lesions, systemic wilting, and blights in plants. in addition, type iii secretion systems are also required for biogenesis of the bacterial flagellum. the hrcq(b) protein, a component of the secretion apparatus of pseudomonas ...	2004	14694203
identification of the liv-i/ls system as the third phenylalanine transporter in escherichia coli k-12.	in escherichia coli, the active transport of phenylalanine is considered to be performed by two different systems, arop and phep. however, a low level of accumulation of phenylalanine was observed in an aromatic amino acid transporter-deficient e. coli strain (deltaarop deltaphep deltamtr deltatna deltatyrp). the uptake of phenylalanine by this strain was significantly inhibited in the presence of branched-chain amino acids. genetic analysis and transport studies revealed that the liv-i/ls syste ...	2004	14702302
rapid and specific detection of salmonella spp. in animal feed samples by pcr after culture enrichment.	a pcr procedure has been developed for routine analysis of viable salmonella spp. in feed samples. the objective was to develop a simple pcr-compatible enrichment procedure to enable dna amplification without any sample pretreatment such as dna extraction or cell lysis. pcr inhibition by 14 different feed samples and natural background flora was circumvented by the use of the dna polymerase tth. this dna polymerase was found to exhibit a high level of resistance to pcr inhibitors present in thes ...	2004	14711627
genetics of zwittermicin a production by bacillus cereus.	zwittermicin a represents a new chemical class of antibiotic and has diverse biological activities, including suppression of oomycete diseases of plants and potentiation of the insecticidal activity of bacillus thuringiensis. to identify genes involved in zwittermicin a production, we generated 4,800 transposon mutants of b. cereus uw101c and screened them for zwittermicin a accumulation. nine mutants did not produce detectable zwittermicin a, and one mutant produced eightfold more than the pare ...	2004	14711631
frequency, size, and localization of bacterial aggregates on bean leaf surfaces.	using epifluorescence microscopy and image analysis, we have quantitatively described the frequency, size, and spatial distribution of bacterial aggregates on leaf surfaces of greenhouse-grown bean plants inoculated with the plant-pathogenic bacterium pseudomonas syringae pv. syringae strain b728a. bacterial cells were not randomly distributed on the leaf surface but occurred in a wide range of cluster sizes, ranging from single cells to over 10(4) cells per aggregate. the average cluster size i ...	2004	14711662
detection of the bacillus anthracis gyra gene by using a minor groove binder probe.	identification of chromosomal markers for rapid detection of bacillus anthracis is difficult because significant chromosomal homology exists among b. anthracis, bacillus cereus, and bacillus thuringiensis. we evaluated the bacterial gyra gene as a potential chromosomal marker for b. anthracis. a real-time pcr assay was developed for the detection of b. anthracis. after analysis of the unique nucleotide sequence of the b. anthracis gyra gene, a fluorescent 3' minor groove binding probe was tested ...	2004	14715750
use of genome selected repeated sequences increases the sensitivity of pcr detection of tropheryma whipplei.	the availability of the tropheryma whipplei genome offers the putative possibility of choosing logical dna targets. we applied a pcr assay (targeting repeated sequences of t. whipplei) to samples from patients with whipple's disease and to those from members of a control group. when compared to the results seen with regular pcr, the sensitivity of repeat pcr was significantly enhanced (p = 0.02) without alteration of its specificity.	2004	14715790
structural alteration of cofactor specificity in corynebacterium 2,5-diketo-d-gluconic acid reductase.	corynebacterium 2,5-diketo-d-gluconic acid reductase (2,5-dkgr) catalyzes the reduction of 2,5-diketo-d-gluconic acid (2,5-dkg) to 2-keto-l-gulonic acid (2-klg). 2-klg is an immediate precursor to l-ascorbic acid (vitamin c), and 2,5-dkgr is, therefore, an important enzyme in a novel industrial method for the production of vitamin c. 2,5-dkgr, as with most other members of the aldo-keto reductase (akr) superfamily, exhibits a preference for nadph compared to nadh as a cofactor in the stereo-spec ...	2004	14718658
the nifl-nifa system: a multidomain transcriptional regulatory complex that integrates environmental signals.		2004	14729684
biosynthesis of agglomerin a: stereospecific incorporation of pro-r- and pro-s-hydrogens at sn-c-3 of glycerol into the branched c(3) moiety.	the biosynthetic origin of the c(3) branched unit of agglomerin a has been investigated. feeding of sn-(3r)- and sn-(3s)-[3-(2)h]glycerols to enterobacter agglomerans pb-6042 followed by (2)h nmr analysis of the resulting agglomerin a revealed that pro-r and pro-s hydrogens at sn-c-3 of glycerol were incorporated stereospecifically into 5e and 5z hydrogens of agglomerin a, respectively. these results imply that the immediate precursor of the c(3) branched unit is not pyruvate, but 1,3-bisphospho ...	2004	14741261
in silico identification and experimental validation of pmrab targets in salmonella typhimurium by regulatory motif detection.	the pmrab (bassr) two-component regulatory system is required for salmonella typhimurium virulence. pmrab-controlled modifications of the lipopolysaccharide (lps) layer confer resistance to cationic antibiotic polypeptides, which may allow bacteria to survive within macrophages. the pmrab system also confers resistance to fe3+-mediated killing. new targets of the system have recently been discovered that seem not to have a role in the well-described functions of pmrab, suggesting that the pmrab- ...	2004	14759259
norm, an erwinia amylovora multidrug efflux pump involved in in vitro competition with other epiphytic bacteria.	blossoms are important sites of infection for erwinia amylovora, the causal agent of fire blight of rosaceous plants. before entering the tissue, the pathogen colonizes the stigmatic surface and has to compete for space and nutrient resources within the epiphytic community. several epiphytes are capable of synthesizing antibiotics with which they antagonize phytopathogenic bacteria. here, we report that a multidrug efflux transporter, designated norm, of e. amylovora confers tolerance to the tox ...	2004	14766544
a new alkali-thermostable azoreductase from bacillus sp. strain sf.	a screening for dye-decolorizing alkali-thermophilic microorganisms resulted in a bacillus sp. strain isolated out of the wastewater drain of a textile finishing company. an nadh-dependent azoreductase of this strain, bacillus sp. strain sf, was found to be responsible for the decolorization of azo dyes. this enzyme was purified by a combination of ammonium sulfate precipitation and anion-exchange and affinity chromatography and had a molecular mass of 61.6 kda and an isoelectric point at ph 5.3 ...	2004	14766562
lead precipitation by vibrio harveyi: evidence for novel quorum-sensing interactions.	three pleiotropic, quorum sensing-defective vibrio harveyi mutants were observed to precipitate soluble pb2+ as an insoluble compound. the compound was purified and subjected to x-ray diffraction and elemental analyses. these assays identified the precipitated compound as pb9(po4)6, an unusual and complex lead phosphate salt that is produced synthetically at temperatures of ca. 200 degrees c. regulation of the precipitation phenotype was also examined. introduction of a luxo::kan allele into one ...	2004	14766565
rpob gene sequence-based identification of aerobic gram-positive cocci of the genera streptococcus, enterococcus, gemella, abiotrophia, and granulicatella.	we developed a new molecular tool based on rpob gene (encoding the beta subunit of rna polymerase) sequencing to identify streptococci. we first sequenced the complete rpob gene for streptococcus anginosus, s. equinus, and abiotrophia defectiva. sequences were aligned with these of s. pyogenes, s. agalactiae, and s. pneumoniae available in genbank. using an in-house analysis program (svarap), we identified a 740-bp variable region surrounded by conserved, 20-bp zones and, by using these conserve ...	2004	14766807
[investigation of the bacteria in the seawater of xisha in the south china sea and their antibiotic sensitivity profile].	to investigate the distribution and antibiotic sensitivity of the bacteria in the seawater of xisha in the south china sea.	2004	15041561
decolorization of azo dyes with enterobacter agglomerans immobilized in different supports by using fluidized bed bioreactor.	immobilized cells of enterobacter agglomerans, able to reduce azo dyes enzymatically, were used as a biocatalyst for the decolorization of synthetic medium containing the toxic azo dye methyl red (mr). this bacterial strain exhibits high ability to completely decolorize 100 mg/l of mr after only 6 h of incubation under aerobic conditions. cells of e. agglomerans were immobilized in calcium alginate, polyacylamide, cooper beech, and vermiculite, and were used for the decolorization of mr from syn ...	2004	15057480