Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| effects of trifluralin on soil microbial populations and the nitrogen fixation activities. | effects of trifluralin on soil microbial populations and the nitrogen fixation activity of nitrogen-fixing bacteria azotobacter chroococcum and bradyrhizobium japonicum and the decomposition of trifluralin by soil microorganisms were studied. trifluralin at lower concentrations from 0.5 mg microg(-1) dry soil to lower than 10.0 mg microg(-1) dry soil appeared to stimulate the growth of soil bacteria, actinomycetes, mould, and the pure cultures of br. japonicum and a. chroococcum. not only the co ... | 2001 | 11599721 |
| population density-dependent regulation of the bradyrhizobium japonicum nodulation genes. | the nodulation genes of bradyrhizobium japonicum are essential for infection and establishment of a nitrogen-fixing symbiosis. here, we demonstrate that plant-produced isoflavones induce nodulation gene expression in a population density-dependent fashion. nodulation gene induction is highest at a low population density and significantly reduced in more dense cultures. a quorum signal molecule in the conditioned medium of b. japonicum cultures mediates this repression. repression in response to ... | 2001 | 11679065 |
| dna sequence and mutational analysis of rhizobitoxine biosynthesis genes in bradyrhizobium elkanii. | we cloned and sequenced a cluster of genes involved in the biosynthesis of rhizobitoxine, a nodulation enhancer produced by bradyrhizobium elkanii. the nucleotide sequence of the cloned 28.4-kb dna region encompassing rtxa showed that several open reading frames (orfs) were located downstream of rtxa. a large-deletion mutant of b. elkanii, usda94 delta rtx::omega 1, which lacks rtxa, orf1 (rtxc), orf2, and orf3, did not produce rhizobitoxine, dihydrorhizobitoxine, or serinol. the broad-host-rang ... | 2001 | 11679318 |
| characterization of the nirk gene encoding the respiratory, cu-containing nitrite reductase of bradyrhizobium japonicum. | the structural gene, nirk, for the respiratory cu-containing nitrite reductase from bradyrhizobium japonicum usda110 has been isolated and sequenced. the deduced amino acid sequence exhibited a high degree of similarity to other cu-containing nitrite reductases from various sources. the full-length protein included a signal peptide for protein export. analysis of the sequence upstream from the structural nirk gene revealed the presence of an anaerobox located 83 base pairs from the putative tran ... | 2001 | 11690645 |
| [cloning and functional analysis of glnb from azospirillum brasilense yu62]. | the glnb gene of a. brasilense yu62 was determined in a 3.7 kb ecori + psti fragment. the glna is located downstream of glnb and an orf for hypothetical protein is on upstream of glnb. the deduced amino acid sequence of pii encoded by glnb is 71%, 77%, 79% and 69% identical to that of k. pneumoniae, bradyrhizobium japonicum, rhizobium leguninosarum and e. coli, respectively. a km-casette was inserted into bglii site of glnb coding region and glnb- mutant was obtained by homologous recombination. ... | 2001 | 11695269 |
| evidence for the production of chemical compounds analogous to nod factor by the silicate bacterium bacillus circulans gy92. | silicate bacteria are generally placed in the species bacillus circulans and are widely used in biological fertilisers and biological leaching. the bacteria can form conspicuous amounts of extracellular polysaccharides in nitrogen-free media or in the presence of substrates with large c/n ratios. using high performance liquid chromatography, we have shown that b. circulans produced a new peak/compound when induced with the plant-to-bacteria signal molecule genistein. this material co-eluted with ... | 2001 | 11716218 |
| improved soybean root association of n-starved bradyrhizobium japonicum. | in this study, we addressed the effects of n limitation in bradyrhizobium japonicum for its association with soybean roots. the wild-type strain lp 3001 grew for six generations with a growth rate of 1.2 day(-1) in a minimal medium with 28 mm mannitol as the carbon source and with the n source [(nh(4))(2)so(4)] limited to only 20 microm. under these conditions, the glutamine synthetase (gs) activity was five to six times higher than in similar cultures grown with 1 or 0.1 mm (nh(4))(2)so(4). the ... | 2001 | 11717284 |
| rhizobitoxine production and symbiotic compatibility of bradyrhizobium from asian and north american lineages of amphicarpaea. | reciprocal inoculations with bradyrhizobium sp. isolates from the north american legume amphicarpaea bracteata (l.) fern. (phaseoleae-glycininae) and from a japanese population of its close relative amphicarpaea edgeworthii (benth.) var. japonica were performed to analyze relative symbiotic compatibility. amphicarpaea edgeworthii plants formed few or no nodules with any north american bradyrhizobial strains isolated from a. bracteata, but all a. bracteata lineages formed effective nitrogen-fixin ... | 2001 | 11718541 |
| induction of h(2)o(2) synthesis by beta-glucan elicitors in soybean is independent of cytosolic calcium transients. | soybean cell suspension cultures have been used to investigate the role of the elevation of the cytosolic ca(2+) concentration in beta-glucan elicitors-induced defence responses, such as h(2)o(2) and phytoalexin production. the intracellular ca(2+) concentration was monitored in transgenic cells expressing the ca(2+)-sensing aequorin. two lines of evidence showed that a transient increase of the cytosolic ca(2+) concentration is not necessarily involved in the induction of h(2)o(2) generation: ( ... | 2001 | 11718714 |
| identification of active site residues in bradyrhizobium japonicum acetyl-coa synthetase. | acetyl-coa synthetase (acs) catalyses the activation of acetate to acetyl-coa in the presence of atp and coa. the gene encoding bradyrhyzobium japonicum acs has been cloned, sequenced, and expressed in escherichia coli. the enzyme comprises 648 amino acid residues with a calculated molecular mass of 71,996 da. the recombinant enzyme was also purified from the transformed e. coli. the enzyme was essentially indistinguishable from the acs of b. japonicum bacteroids as to the criteria of polyacryla ... | 2001 | 11726281 |
| methylotrophic methylobacterium bacteria nodulate and fix nitrogen in symbiosis with legumes. | rhizobia described so far belong to three distinct phylogenetic branches within the alpha-2 subclass of proteobacteria. here we report the discovery of a fourth rhizobial branch involving bacteria of the methylobacterium genus. rhizobia isolated from crotalaria legumes were assigned to a new species, "methylobacterium nodulans," within the methylobacterium genus on the basis of 16s ribosomal dna analyses. we demonstrated that these rhizobia facultatively grow on methanol, which is a characterist ... | 2001 | 11114919 |
| numerical taxonomy of sarothamnus scoparius rhizobia. | twenty nodule isolates from sarothamnus scoparius (broom) growing in poland and nine strains from plants growing in japan were studied for phenotypic properties, plasmid presence, phage sensitivity, and host plant specificity. by numerical analysis of phenotypic properties, it was found that the studied nodule bacteria, originating from geographically different countries, constitute two separate groups affiliated to the bradyrhizobium cluster. the membership of s. scoparius rhizobia in the brady ... | 2001 | 11116393 |
| characterization of the common nodulation genes of the photosynthetic bradyrhizobium sp. ors285 reveals the presence of a new insertion sequence upstream of noda. | we isolated and characterized noda genes from photosynthetic and non-photosynthetic rhizobia nodulating the legume genus aeschynomene, and found that the noda sequence from photosynthetic stem-nodulating bacteria was phylogenetically distant from the other already described noda genes. characterization of the photosynthetic strain ors285 common nod gene cluster (nodabc) showed, upstream of noda, the presence of a new insertion sequence element belonging to the is3 family and specific to a group ... | 2001 | 11150670 |
| small-subunit rrna genotyping of rhizobia nodulating australian acacia spp. | the structure of rhizobial communities nodulating acacia in southeastern australia from south queensland to tasmania was investigated by a molecular approach. a total of 118 isolates from nodule samples from 13 different acacia species collected at 44 sites were characterized by small-subunit (ssu) ribosomal dna (rdna) pcr-restriction fragment length polymorphism analysis. nine rhizobial genomospecies were identified, and these taxa corresponded to previously described genomospecies (b. lafay an ... | 2001 | 11133471 |
| synthesis of a low-molecular-weight form of exopolysaccharide by bradyrhizobium japonicum usda 110. | a novel extracellular low-molecular-weight polysaccharide was detected as a contaminant within extracellular cyclic beta-1,6-beta-1,3-glucan preparations from bradyrhizobium japonicum usda 110 cultures. compositional analysis, methylation analysis, and nuclear magnetic resonance analysis revealed that this low-molecular-weight polysaccharide was composed of the same pentasaccharide repeating unit previously described for the high-molecular-weight form of the exopolysaccharide (eps) synthesized b ... | 2001 | 11157281 |
| one of two hemn genes in bradyrhizobium japonicum is functional during anaerobic growth and in symbiosis. | previously, we screened the symbiotic gene region of the bradyrhizobium japonicum chromosome for new nifa-dependent genes by competitive dna-rna hybridization (a. nienaber, a. huber, m. göttfert, h. hennecke, and h. m. fischer, j. bacteriol. 182:1472-1480, 2000). here we report more details on one of the genes identified, a hemn-like gene (now called hemn(1)) whose product exhibits significant similarity to oxygen-independent coproporphyrinogen iii dehydrogenases involved in heme biosynthesis in ... | 2001 | 11157943 |
| potential symbiosis-specific genes uncovered by sequencing a 410-kilobase dna region of the bradyrhizobium japonicum chromosome. | the physical and genetic map of the bradyrhizobium japonicum chromosome revealed that nitrogen fixation and nodulation genes are clustered. because of the complex interactions between the bacterium and the plant, we expected this chromosomal sector to contain additional genes that are involved in the maintenance of an efficient symbiosis. therefore, we determined the nucleotide sequence of a 410-kb region. the overall g+c nucleotide content was 59.1%. using a minimum gene length of 150 nucleotid ... | 2001 | 11157954 |
| phylogenetic and dna-dna hybridization analyses of bradyrhizobium species. | the 16s rdna sequence of bradyrhizobium liaoningense was determined and analysed together with sequences of other bradyrhizobium species and related taxa. in addition, dna-dna hybridizations were performed between representative strains of the three bradyrhizobium species. bradyrhizobium liaoningense is genotypically highly related to bradyrhizobium japonicum, whereas bradyrhizobium elkanii is more distantly related to these two species. the fact that afipia, agromonas, blastobacter, nitrobacter ... | 2001 | 11211247 |
| novel methylated triterpenoids of the gammacerane series from the nitrogen-fixing bacterium bradyrhizobium japonicum usda 110. | the nitrogen-fixing, symbiotic root-nodule forming bacterium bradyrhizobium japonicum usda 110 contained gammacerane derivatives next to triterpenoids of the hopane series. diploptene, diplopterol, 2 beta-methyldiplopterol, aminobacteriohopanetriol and adenosylhopane were accompanied by tetrahymanol and the corresponding novel methylated homologues 2 beta-methyltetrahymanol, 20 alpha-methyltetrahymanol, and 2 beta,20 alpha-dimethyltetrahymanol. incorporation of [(2)h(3)]methyl-l-methionine indic ... | 2001 | 11231284 |
| supply of o2 regulates demand for o2 and uptake of malate by n2-fixing bacteroids from soybean nodules. | bacteroids, prepared anaerobically from soybean root nodules by fractional centrifugation or by sucrose or percoll density-gradient methods, were retained within a stirred, flow-through reaction chamber and used to determine rates of respiration and n2 fixation at various rates of o2 supply. liquid reaction solutions containing malate, oxyleghaemoglobin, dissolved n2 and various levels of dissolved o2 were passed through the reaction chamber at measured rates of flow. the relative oxygenation of ... | 2001 | 11238973 |
| partial purification and characterization of pyruvate kinase from the plant fraction of soybean root nodules. | pyruvate kinase (pk, ec 2.7.1.40) was partially purified from the plant cytosolic fraction of n2-fixing soybean (glycine max [l.] merr.) root nodules. the partially purified pk preparation was completely free of contamination by phosphoenolpyruvate carboxylase (pepc, ec 4.1.1.31), the other major phosphoenolpyruvate (pep)-utilizing enzyme in legume root nodules. latency experiments with sonicated nodule extracts showed that bradyrhizobium japonicum bacteroids do not express either pk or pepc act ... | 2001 | 11240911 |
| the root-nodule symbiosis between sarothamnus scoparius l. and its microsymbionts. | when nitrogen fixing root nodules are formed, sarothamnus scoparius (broom) is inoculated with its microsymbionts. nodules studied under light and electron microscopy exhibited typical indeterminate nodule histology with apical, persistent meristem, age gradient of nodule tissues, and open vascular bundles, and also with some particular features such as: the presence of mitotic activity in the infected meristematic cells, lack of infection threads, distribution of bacteria by process of host cel ... | 2001 | 11816984 |
| phylogenetic analysis of microbial diversity in the rhizoplane of oilseed rape (brassica napus cv. westar) employing cultivation-dependent and cultivation-independent approaches. | the structure of the microbial rhizoplane community of the important crop plant oilseed rape was studied by using a culture-dependent as well as a culture-independent approach based on 16s rdna amplification. after isolation of the microbial community from the rhizoplane of oilseed rape (brassica napus cv. westar), the collected suspension was divided into two parts. one part was used for cultivation of bacteria onto three different growth media to establish a culture collection. from the other ... | 2001 | 12024277 |
| [effect of ph on nodulation of soybean rhizobia from weifang and huayuankou soils]. | the effect of ph on the nodulation of sinorhizobium fredii and bradyrhizobium japonicum was examined by analyzing the indigent soybean rhizobia, predominant indigent rhizobia, and specific rhizobia, respectively. the results showed that very acid and very alkaline environment could retard the nodulation and inhibit the growth of the rhizobia. sinorhizohium fredii could endure environment more strongly than bradyrhizobium japonicum, and had a high competitive nodulation capacity. bradyhizobium ja ... | 2001 | 11758403 |
| phylogenies of atpd and reca support the small subunit rrna-based classification of rhizobia. | the current classification of the rhizobia (root-nodule symbionts) assigns them to six genera. it is strongly influenced by the small subunit (16s, ssu) rrna molecular phylogeny, but such single-gene phylogenies may not reflect the evolution of the genome as a whole. to test this, parts of the atpd and reca genes have been sequenced for 25 type strains within the alpha-proteobacteria, representing species in rhizobium, sinorhizobium, mesorhizobium, bradyrhizobium, azorhizobium, agrobacterium, ph ... | 2001 | 11760945 |
| the bradyrhizobium japonicum hsfa gene exhibits a unique developmental expression pattern in cowpea nodules. | the bradyrhizobium japonicum host-specific fixation gene hsfa was identified as essential for nitrogen fixation on cowpea, but not required for nitrogen fixation on soybean or siratro. the dna sequence of the hsfa promoter contains a consensus rpon, -24/-12 binding site, suggesting the involvement of a regulatory protein that binds to an upstream activating sequence (uas). to further explore the regulation of this interesting gene, serial deletions of the hsfa promoter were made and fused with t ... | 2001 | 11763126 |
| co-inoculation with bacillus sp. cect 450 improves nodulation in phaseolus vulgaris l. | the strain bacillus sp. cect 450 increased nodulation on bean (phaseolus vulgaris l.) when co-inoculated with rhizobium tropici ciat 899. this positive effect occured under controlled conditions on perlite-vermiculite, sand, or in a mixture of soil and sand. this increase was also observed in a field assay. nodulation kinetic studies suggested that the synergistic effect is pronounced during the latter stages of cultivation. in contrast, the same bacteria co-inoculated with bradyrhizobium japoni ... | 2001 | 11766056 |
| bradyrhizobium japonicum mutants with enhanced sensitivity to genistein resulting in altered nod gene regulation. | bradyrhizobium japonicum mutants with altered nod gene induction characteristics were isolated by screening mutants for genistein-independent nod gene expression. plasmid pzb32, carrying a nody::lacz transcriptional gene fusion, was introduced into b. japonicum cells that had been subjected to uv mutagenesis. ten independent transformants producing a blue color on plates containing 5bromo-4chloro-3indolyl-beta-d-galactopyranoside but lacking genistein, indicative of constitutive expression of th ... | 2001 | 11768535 |
| denaturing gradient gel electrophoresis analyses of microbial community from field-scale composter. | the diversity of microbial community during the decomposition of waste in a field-scale composter (hazaka system) was investigated by denaturing gradient gel electrophoresis (dgge). the composter operates at a high temperature through a self-heating system, creating a thermophilic (60-76 degrees c) stage during the initial phase and a mesophilic (45 degrees c) stage towards the later phase of the composting period. the ph of the system (ph 7.75-8.10) did not vary significantly during the process ... | 2001 | 16232968 |
| study of the inducer effect of molasses and soybean cake on synthesis and excretion of nodulation factors in different strains of bradyrhizobium japonicum. | it is known that the synthesis of nodulation factors by the bacteria within the genera rhizobium is induced by different compounds, mainly of flavonoid nature exudated by the legume plants. the capacity of different compounds to act as inducers of nod genes on three bradyrhizobium japonicum strains was studied in this paper and this effect was compared using two concentrations of the inducer. induced nod factors were observed among the strains exposed to the inducers. the profiles and amount of ... | 2001 | 17061566 |
| characterization of lps mutants of peanut specific bradyrhizobium (gn17). | two mutants of bradyrhizobium sp. (arachis) strain gn17 having altered lipopolysaccharide (lps) composition were isolated upon random tn5 mutagenesis to study their binding with peanut root lectin (pra ii). these mutant strains designated as gn17m1 and gn17m2 produced rough colonies and showed autoagglutination. flow cytometric analyses indicated that strain gn17m1 bind to pra ii with highest efficiency. both the mutants synthesized only high molecular weight lipopolysaccharides as observed by s ... | 2002 | 14707486 |
| interaction between the bacterial iron response regulator and ferrochelatase mediates genetic control of heme biosynthesis. | the heme biosynthetic pathway culminates with the insertion of iron into protoporphyrin catalyzed by ferrochelatase. the bradyrhizobium japonicum iron response regulator (irr) protein represses the pathway at an early step under iron limitation to prevent protoporphyrin synthesis from exceeding iron availability. here, we show that irr interacts directly with ferrochelatase and responds to iron via the status of heme and protoporphyrin localized at the site of heme synthesis. in the presence of ... | 2002 | 11804594 |
| characterization of rhizobia that nodulate legume species of the genus lespedeza and description of bradyrhizobium yuanmingense sp. nov. | legume species belonging to the genus lespedeza are annual or perennial herb or shrub plants that grow in the northern hemisphere. they are known for the formation of root nodules, but little information is available about their microsymbionts. in this study, 58 root-nodule isolates from lespedeza spp., obtained from china and the usa, were characterized using numerical taxonomic analysis of phenotypic features, sds-page analysis of whole-cell proteins, dna-dna hybridization, 16s rrna gene seque ... | 2002 | 12508891 |
| prediction and overview of the rpon-regulon in closely related species of the rhizobiales. | in the rhizobia, a group of symbiotic gram-negative soil bacteria, rpon (sigma54, sigman, ntra) is best known as the sigma factor enabling transcription of the nitrogen fixation genes. recent reports, however, demonstrate the involvement of rpon in other symbiotic functions, although no large-scale effort has yet been undertaken to unravel the rpon-regulon in rhizobia. we screened two complete rhizobial genomes (mesorhizobium loti, sinorhizobium meliloti) and four symbiotic regions (rhizobium et ... | 2002 | 12537565 |
| characterization of ndvd, the third gene involved in the synthesis of cyclic beta-(1 --> 3),(1 --> 6)-d-glucans in bradyrhizobium japonicum. | previously, we identified two genes in bradyrhizobium japonicum (ndvb, ndvc) that are required for cyclic beta-(1 --> 3),(1 --> 6)-d-glucan synthesis and successful symbiotic interaction with soybean (glycine max). in this study, we report a new open reading frame (orf1) located in the intergenic region between ndvb and ndvc, which is essential for beta-glucan synthesis and effective nodulation of g. max. this new gene is designated ndvd (nodule development). the ndvd translation product has a p ... | 2002 | 12556128 |
| phenotypic and genotypic diversity of rhizobia nodulating pterocarpus erinaceus and p. lucens in senegal. | a total of fifty root nodules isolates of fast-growing and slow growing rhizobia from pterocarpus ennaceus and pterocarpus lucens respectively native of sudanean and sahelian regions of senegal were characterized. these isolates were compared to representative strains of known rhizobial species. twenty-two new isolates were slow growers and twenty-eight were fast growers. a polyphasic approach was performed including comparative total protein sodium dodecyl sulphate polyacrylamide gel (sds-page) ... | 2002 | 12583718 |
| complete genomic sequence of nitrogen-fixing symbiotic bacterium bradyrhizobium japonicum usda110. | the complete nucleotide sequence of the genome of a symbiotic bacterium bradyrhizobium japonicum usda110 was determined. the genome of b. japonicum was a single circular chromosome 9,105,828 bp in length with an average gc content of 64.1%. no plasmid was detected. the chromosome comprises 8317 potential protein-coding genes, one set of rrna genes and 50 trna genes. fifty-two percent of the potential protein genes showed sequence similarity to genes of known function and 30% to hypothetical gene ... | 2002 | 12597275 |
| complete genomic sequence of nitrogen-fixing symbiotic bacterium bradyrhizobium japonicum usda110 (supplement). | 2002 | 12597279 | |
| [genetic diversity and phylogeny of rhizobia isolated from peanut (arachis hypogaea)]. | forty three rhizobium strains isolated from peanut (arachis hypogaea) and 15 reference strains from other genus and species were analyzed by the method of 16s rrna rflp, 16s rrna sequencing and 16s-23s igs pcr rflp. the results of the 16s rrna rflp shown that 43 strains tested were all ascribed to the genus of bradyrhizobium phylogenetically. strains tested were adjacent to the b. japonicum and far from b. elkanii 16s rrna genotype. the genotypes generated by the 4 restriction endonucleases, mbo ... | 2002 | 12693105 |
| identification of a cdna from the arbuscular mycorrhizal fungus glomus intraradices that is expressed during mycorrhizal symbiosis and up-regulated by n fertilization. | a cdna library was constructed with rna from glomus intraradices-colonized lettuce roots and used for differential screening. this allowed the identification of a cdna (gi-1) that was expressed only in mycorrhizal roots and was of fungal origin. the function of the gene product is unknown, because gi-1 contained a complete open reading frame that was predicted to encode a protein of 157 amino acids which only showed little homology with glutamine synthetase from helicobacter pylori. the time-cou ... | 2002 | 12026174 |
| structure of malonamidase e2 reveals a novel ser-cisser-lys catalytic triad in a new serine hydrolase fold that is prevalent in nature. | a large group of hydrolytic enzymes, which contain a conserved stretch of approximately 130 amino acids designated the amidase signature (as) sequence, constitutes a super family that is distinct from any other known hydrolase family. as family enzymes are widespread in nature, ranging from bacteria to humans, and exhibit a variety of biological functions. here we report the first structure of an as family enzyme provided by the crystal structure of malonamidase e2 from bradyrhizobium japonicum. ... | 2002 | 12032064 |
| evolutionary relationship of phototrophic bacteria in the alpha-proteobacteria based on farnesyl diphosphate synthase. | partial sequences of farnesyl diphosphate (fpp) synthase genes derived from the rhodobacter-rhodovulum group and from the rhodopseudomonas palustris-bradyrhizobium japonicum group of the alpha-proteobacteria were subjected to phylogenetic analysis to investigate the relationships of phototrophic and non-phototrophic bacteria in the alpha-proteobacteria . the four rhodovulum species formed a monophyletic group within the rhodobacter cluster, and agrobacterium ferrugineum iam 12616(t) intermingled ... | 2002 | 12055311 |
| reassessment of major products of n2 fixation by bacteroids from soybean root nodules. | nh3/ was the principal product from soybean bacteroids, prepared by various procedures, when assayed in solution in a flow chamber under n2 fixation conditions. in addition, small quantities of alanine were produced (reaching 20% of nh3/ under some conditions). some 15n was assimilated by bacteroids purified from soybean root nodules on percoll density gradients and shaken with 15n2 and 0.008 atm o2. under these conditions, accounted for 93% of the (15)n fixed into the soluble fraction. this fra ... | 2002 | 12055315 |
| tfda-like genes in 2,4-dichlorophenoxyacetic acid-degrading bacteria belonging to the bradyrhizobium-agromonas-nitrobacter-afipia cluster in alpha-proteobacteria. | the 2,4-dichlorophenoxyacetate (2,4-d)/alpha-ketoglutarate dioxygenase gene (tfda) homolog designated tfdaalpha was cloned and characterized from 2,4-d-degrading bacterial strain rd5-c2. this japanese upland soil isolate belongs to the bradyrhizobium-agromonas-nitrobacter-afipia cluster in the alpha subdivision of the class proteobacteria on the basis of its 16s ribosomal dna sequence. sequence analysis showed 56 to 60% identity of tfdaalpha to representative tfda genes. a male-tfdaalpha fusion ... | 2002 | 12089027 |
| the effect of nitrogen nutrition on cluster root formation and proton extrusion by lupinus albus. | nitrogen nutrition can influence cluster root formation in many wild species, but the effect of n form on cluster root formation and root exudation by white lupin is not known. in a solution culture study, we examined the effect of n nutrition (ammonium, nitrate, both or n2 fixation) on cluster root formation and h+ extrusion by white lupin plants under deficient and adequate p supply. the number of cluster roots increased greatly when plants were supplied with i microm p compared with 50 microm ... | 2002 | 12096804 |
| sinorhizobium fredii isolates can be specifically identified by a 260 bp fragment from the nolxwbtuv locus. | a pair of primers homologous to the nolxwbtuv locus generated a 260 bp fragment by pcr only in the presence of sinorhizobium fredii template dna of different quality. this resulted in a fast and accurate method for the identification of s. fredii either from pure dna, whole bacterial cells or nodule extracts. by means of two pcr fragments, one specific for s. fredii (260-bp) and the other specific for bradyrhizobium japonicum (rsalpha), we found that s. fredii strain smh12 and b. japonicum e109 ... | 2002 | 12111156 |
| effect of short-term n(2) deficiency on expression of the ureide pathway in cowpea root nodules. | root systems of 28-d-old cowpea (vigna unguiculata l. walp cv vita 3: bradyrhizobium sp. strain cb756) plants bearing nitrogen-fixing nodules in sand culture were exposed to an atmosphere of ar:o(2) (80:20, v/v) for 48 h and then returned to air. root systems of control plants were maintained in air throughout. nodules were harvested at the same times in control and ar:o(2)-treated root systems. activities of two enzymes of de novo purine synthesis, glycinamide ribonucleotide transformylase (gar ... | 2002 | 12114575 |
| the soybean gmn6l gene encodes a late nodulin expressed in the infected zone of nitrogen-fixing nodules. | previously, we determined the n-terminal amino acid sequences of a number of putative peribacteroid membrane proteins from soybean. here, we report the cloning of a gene, gmn6l, that encodes one of these proteins. the protein encoded by gmn6l is similar in sequence to mtn6, an early nodulin expressed in medicago truncatula roots in response to infection by sinorhizobium meliloti. the gmn6l gene was strongly expressed in mature nodules but not in other plant organs. gmn6l protein was first detect ... | 2002 | 12118878 |
| structure of ccmg/dsbe at 1.14 a resolution: high-fidelity reducing activity in an indiscriminately oxidizing environment. | ccmg is unlike other periplasmic thioredoxin (trx)-like proteins in that it has a specific reducing activity in an oxidizing environment and a high fidelity of interaction. these two unusual properties are required for its role in c-type cytochrome maturation. the crystal structure of ccmg reveals a modified trx fold with an unusually acidic active site and a groove formed from two inserts in the fold. deletion of one of the groove-forming inserts disrupts c-type cytochrome formation. two unique ... | 2002 | 12121652 |
| a critical motif for oligomerization and chaperone activity of bacterial alpha-heat shock proteins. | oligomerization into multimeric complexes is a prerequisite for the chaperone function of almost all alpha-crystallin type heat shock proteins (alpha-hsp), but the molecular details of complex assembly are poorly understood. the alpha-hsp proteins from bradyrhizobium japonicum are suitable bacterial models for structure-function studies of these ubiquitous stress proteins. they fall into two distinct classes, a and b, display chaperone activity in vitro and form oligomers of approximately 24 sub ... | 2002 | 12135498 |
| yellow lupine gene encoding stearoyl-acp desaturase--organization, expression and potential application. | a gene for the delta9 desaturase specific to stearoyl-acp (acyl carrier protein) was identified from yellow lupine (lupinus luteus) cdna and genomic libraries through the differential display method. the desaturase transcript appears in plants infected with bradyrhizobium sp. (lupinus) as revealed by northern hybridization, rt-pcr and expression of beta-glucuronidase under the desaturase promoter. a small amount of desaturase transcript was also detected in uninfected plants, which suggests that ... | 2002 | 12136953 |
| survival and nutritional requirements of three bacteria isolated from ultrapure water. | bacteria isolated previously from ultrapure water (upw) systems were examined for their ability to survive in upw, with the ultimate goal of elucidating potential carbon and energy sources for the bacteria. two strains of ralstonia pickettii isolated from different areas within the upw system (pretreatment and polishing loop, and referred to as strains 3a1 and mf254a, respectively) and a strain of bradyrhizobium sp. were compared to increase our understanding of the fundamental behavior of bacte ... | 2002 | 12161774 |
| the major nod factor of bradyrhizobium japonicum promotes early growth of soybean and corn. | greenhouse experiments were conducted to evaluate the effect of nod factor nod bj-v (c18:1, mefuc) of badyrhizobium japonicum on the growth of soybean and corn. three-day-old seedlings of soybean and corn were grown in hydroponic solutions containing four concentrations (0, 10(-7), 10(-9) or 10(-11) m) of nod factor. after 7 d of treatment, nod factor enhanced soybean and corn biomass. nod factor elicited profound effects on root growth resulting in 34-44% longer roots in soybean. more detailed ... | 2002 | 12177132 |
| conflicting phylogeographic patterns in rrna and nifd indicate regionally restricted gene transfer in bradyrhizobium. | major differences in evolutionary relationships of the 16s rrna gene and the nitrogenase alpha-subunit gene (nifd) were observed among 38 strains of bradyrhizobium sp. nodule bacteria from north america, central america, asia and australia. two lineages were evident in the 16s rrna phylogeny representing strains related to bradyrhizobium japonicum (29 isolates) or bradyrhizobium elkanii (9 isolates). both clades were distributed across most or all of the geographic regions sampled. by contrast, ... | 2002 | 12177349 |
| identification of a soybean protein that interacts with gaga element dinucleotide repeat dna. | dinucleotide repeat dna with the pattern (ga)(n)/(tc)(n), so-called gaga elements, control gene expression in animals, and are recognized by a specific regulatory protein. here, a yeast one-hybrid screen was used to isolate soybean (glycine max) cdna encoding a gaga-binding protein (gbp) that binds to (ga)(n)/(ct)(n) dna. soybean gbp was dissimilar from the gaga factor of drosophila melanogaster. recombinant gbp protein did not bind to dinucleotide repeat sequences other than (ga)(n)/(ct)(n). gb ... | 2002 | 12177492 |
| a novel two-component system of bradyrhizobium japonicum: elms and elmr are encoded in diverse orientations. | by completely sequencing the bradyrhizobium japonicum insert of cosmid c27, a pair of genes was identified which was found to be highly similar to prokaryotic two-component systems. the genes of the putative regulator protein (214 aa), elmr, and the putative sensor histidine kinase (470 aa), elms, are divergently oriented with their putative translational start sites separated by only 85 nucleotides. this new locus is located about 10 kb upstream of the sips signal peptidase allele. the highest ... | 2002 | 12180351 |
| survival of several rhizobium/bradyrhizobium strains on different inoculant formulations and inoculated seeds. | the effect of a variety factors on the survival of several rhizobia strains on inoculants and inoculated seeds has been evaluated. since the rhizobia strains showed different cell-density-evolution patterns on peat-based inoculants and on inoculated seeds, several inoculant formulations with highly effective rhizobium/bradyrhizobium strains (for lupinus, hedysarum, phaseolus and glycine max.) were monitored under the following storage conditions: (a) the inoculants were kept refrigerated (at 4 d ... | 2002 | 12180784 |
| diversity among bradyrhizobium isolates nodulating yardlong bean and sunnhemp in guam. | to isolate and characterize bradyrhizobia that nodulate yardlong bean and sunnhemp in guam. | 2002 | 12234340 |
| [chemotaxis of bradyrhizobium japonicum to various organic compounds]. | the investigation of the chemotactic response of bradyrhizobium japonicum to amino acids, carbohydrates, multiatomic alcohols, organic acids, and soybean extracts showed that the extracts of some soybean varieties (chernoburaya and beskluben'kovaya) contain repellents. this indicates that the soybeans of host plants contain effectors that may play a role at the early stages of their interaction with nodule bacteria. | 2002 | 12244714 |
| ultrafast ligand rebinding in the heme domain of the oxygen sensors fixl and dos: general regulatory implications for heme-based sensors. | heme-based oxygen sensors are part of ligand-specific two-component regulatory systems, which have both a relatively low oxygen affinity and a low oxygen-binding rate. to get insight into the dynamical aspects underlying these features and the ligand specificity of the signal transduction from the heme sensor domain, we used femtosecond spectroscopy to study ligand dynamics in the heme domains of the oxygen sensors fixl from bradyrhizobium japonicum (fixlh) and dos from escherichia coli (dosh). ... | 2002 | 12271121 |
| diversity and evolution of hydrogenase systems in rhizobia. | uptake hydrogenases allow rhizobia to recycle the hydrogen generated in the nitrogen fixation process within the legume nodule. hydrogenase (hup) systems in bradyrhizobium japonicum and rhizobium leguminosarum bv. viciae show highly conserved sequence and gene organization, but important differences exist in regulation and in the presence of specific genes. we have undertaken the characterization of hup gene clusters from bradyrhizobium sp. (lupinus), bradyrhizobium sp. (vigna), and rhizobium tr ... | 2002 | 12324339 |
| [formation and function of the bean-rhizobium symbiosis in soy plants upon introduction of strains of azotobacter and bacillus species]. | the effects of bacteria belonging to the genera azotobacter and bacillus in a mixed culture with bradyrhizobium japonicum strains on formation and function of the legume-rhizobium symbiosis of soybean plants were studied. the data showed that the bacterial compositions b. japonicum 634b + b. subtilis 5, b. japonicum 634b + a. chroococcum 20, and b. japonicum 10k + a. vinelandii 56 with a cell ratio of 1:0.1 increased the number and weight of root nodules as well as the height and weight of the a ... | 2002 | 12325300 |
| a novel hmg a-like protein binds differentially to the at-rich regions located in the far distal and proximal parts of a soybean glutamine synthetase gene (gs15) promoter. | in soybean (glycine max l.) ammonium provided externally or as the result of symbiotic nitrogen fixation stimulates the transcription of gs15, a gene encoding cytosolic glutamine synthetase. strong constitutive positive expression (scpe), silencer-like and organ-specific elements, located respectively in the distal, the central and the proximal region of the promoter are required to control the ammonium responsiveness of the gene expression [tercé-laforgue et al. (1999) plant mol. biol. 39: 551] ... | 2002 | 12354918 |
| malonate metabolism: biochemistry, molecular biology, physiology, and industrial application. | malonate is a three-carbon dicarboxylic acid. it is well known as a competitive inhibitor of succinate dehydrogenase. it occurs naturally in biological systems, such as legumes and developing rat brains, which indicates that it may play an important role in symbiotic nitrogen metabolism and brain development. recently, enzymes that are related to malonate metabolism were discovered and characterized. the genes that encode the enzymes were isolated, and the regulation of their expression was also ... | 2002 | 12359084 |
| in search of a bacterial species definition. 1997. | 2002 | 12362904 | |
| structure-based mechanism of o2 sensing and ligand discrimination by the fixl heme domain of bradyrhizobium japonicum. | structures of the bradyrhizobium japonicum fixl heme domain have been determined in the absence and presence of specific ligands to elucidate the detailed features of its o2 sensing mechanism. the putative roles of spin-state and steric hindrance were evaluated by the structure determination of ferrous co-bound bjfixlh and correlating its features with other ligand-bound structures. as found for no-bjfixlh, no protein conformational change was observed in co-bjfixlh, suggesting a more complicate ... | 2002 | 12390021 |
| construction of green fluorescent protein (gfp)-marked strains of bradyrhizobium for ecological studies. | to introduce the gfp gene encoding green fluorescent protein (gfp) into bradyrhizobia for their identification in nodules, soil and carrier-based inoculants. | 2002 | 12392530 |
| bradyoxetin, a unique chemical signal involved in symbiotic gene regulation. | bradyrhizobium japonicum is a symbiotic bacterium that nodulates soybean. critical for the infection and establishment of this symbiosis are the bacterial nodulation genes (nod, nol, noe), which are induced in the presence of plant produced isoflavones. transcription of the nodulation genes is also controlled in a population density-dependent fashion. expression of the nod genes is maximal at low population densities, and decreases significantly at higher culture densities. population density co ... | 2002 | 12393811 |
| siderophoregenic bradyrhizobia boost yield of soybean. | after screening for siderophore (microbial iron chelator) production, of seven available cultures of soybean (glycine max l.) root nodule bradyrhizobia, one strain, bradyrhizobium japonicum ncim 2746, was selected to confirm its phytopathogenic suppression and soybean growth promotion. based on chromatographic and spectrophotometric studies, two different siderophores, a hydroxamate type (mw 734) and another catecholate type (mw 1000), were observed. randomized block design (rbd) analysis of sic ... | 2002 | 12396119 |
| in vitro induction of lipo-chitooligosaccharide production in bradyrhizobium japonicum cultures by root extracts from non-leguminous plants. | bradyrhizobium japonicum can form a n2-fixing symbiosis with compatible leguminous plants. it can also act as a plant-growth promoting rhizobacterium (pgpr) for non-legume plants, possibly through production of lipo-chitooligosaccharides (lcos), which should have the ability to induce disease resistance responses in plants. the objective of this work was to determine whether non-leguminous crop plants can induce lco formation by b. japonicum cultures. cultures treated with root extracts of soybe ... | 2002 | 12398283 |
| genomic fingerprinting of bradyrhizobium japonicum isolates by rapd and rep-pcr. | genetic diversity of indigenous bradyrhizobium japonicum population in croatia was studied by using different pcr-based fingerprinting methods. characteristic dna profiles for 20 b. japonicum field isolates and two reference strains were obtained using random primers (rapd) and two sets of repetitive primers (rep- and eric-pcr). in comparison with the rep, the eric primer set generates fingerprints of lower complexity, but still several strain-specific bands were detected. different b. japonicum ... | 2002 | 12398292 |
| analysis of genomic diversity among photosynthetic stem-nodulating rhizobial strains from northeast argentina. | the genomic diversity among photosynthetic rhizobia from northeast argentina was assessed. forty six isolates obtained from naturally occurring stem and root nodules of aeschynomene rudis plants were analyzed by three molecular typing methods with different levels of taxonomic resolution: repetitive sequence-based pcr (rep-pcr) genomic fingerprinting with box and rep primers, amplified 16s rdna restriction analysis (ardra), and 16s-23s rdna intergenic spacer-restriction fragment length polymorph ... | 2002 | 12421080 |
| formation of pyridine nucleotides under symbiotic and non-symbiotic conditions between soybean nodules and free-living rhizobia. | enzymatic regulation of pyricline nucleotide formation, under symbiotic and non-symbiotic conditions, was analyzed using soybeans (glycine max l. cv. 'akisengoku') and rhizobia (bradyrhizobia japonicum strain a1017), respectively. it was found that levels of pyridine nucleotides in bacteroids in root nodules were different from those in free-living cells of rhizobia. this difference was associated with differences in activities of enzymes involved in the pathway from l-tryptophan to nad and nadp ... | 2002 | 12423884 |
| characterization of the norcbqd genes, encoding nitric oxide reductase, in the nitrogen fixing bacterium bradyrhizobium japonicum. | the genes norcbqd that encode the bc-type nitric oxide reductase from bradyrhizobium japonicum usda110 have been isolated and characterized. norc and norb encode the cytochrome c-containing subunit ii and cytochrome b-containing subunit i of nitric oxide reductase, respectively. norq encodes a protein with an atp/gtp-binding motif, and the predicted nord gene product shows similarity with nord from other denitrifiers. mutational analysis indicates that the two structural norc and norb genes are ... | 2002 | 12427946 |
| partial 16s rrna gene sequence diversity and numerical taxonomy of slow growing pigeonpea (cajanus cajan l millsp) nodulating rhizobia. | an investigation was carried out to determine the diversity of 30 isolates of slow growing pigeonpea nodulating rhizobia based on variations in partial sequences of the 16s rrna gene and numerical analysis of 80 phenotypic traits. phylogenetic analysis using molecular sequences of 23 isolates showed that arpe1 separated from the other isolates at an average distance of >14% divergence level. the other isolates were all within 5% divergence from each other but separated into four main groups, wit ... | 2002 | 12435494 |
| structural adaptations in a membrane enzyme that terminates endocannabinoid signaling. | cellular communication in the nervous system is mediated by chemical messengers that include amino acids, monoamines, peptide hormones, and lipids. an interesting question is how neurons regulate signals that are transmitted by membrane-embedded lipids. here, we report the 2.8 angstrom crystal structure of the integral membrane protein fatty acid amide hydrolase (faah), an enzyme that degrades members of the endocannabinoid class of signaling lipids and terminates their activity. the structure o ... | 2002 | 12459591 |
| transcriptional response of soybean suspension-cultured cells induced by nod factors obtained from bradyrhizobium japonicum usda110. | genes responding to nod factors were picked up by the application of a differential display method for soybean suspension-cultured cells. forty-five cdna fragments derived from such genes were detected. seven fragments (ssc1-ssc7) were successfully cloned. the putative product of genes corresponding to ssc1 was estimated to be a disease-resistance protein relating to the induction of the plant defense response against pathogens, and that corresponding to ssc7 was a sucrose transporter. amino aci ... | 2002 | 12461131 |
| symbiotic root nodule bacteria isolated from yam bean (pachyrhizus erosus). | a total of 25 isolates from root nodules of yam bean (pachyrhizus erosus l. urban), a tuber-producing leguminous plant, were characterized. all isolates formed effective nodules mainly on lateral roots while edible tubers were developed on the taproot. the root nodules formed were identified as the typical determinate type. by an analysis of the partial sequences of the 16s rrna gene (approximately 300 bp) of 10 strains which were selected randomly, the isolated root nodule bacteria of yam bean ... | 2002 | 12469317 |
| generating and exploiting polarity in bacteria. | bacteria are often highly polarized, exhibiting specialized structures at or near the ends of the cell. among such structures are actin-organizing centers, which mediate the movement of certain pathogenic bacteria within the cytoplasm of an animal host cell; organized arrays of membrane receptors, which govern chemosensory behavior in swimming bacteria; and asymmetrically positioned septa, which generate specialized progeny in differentiating bacteria. this polarization is orchestrated by comple ... | 2002 | 12471245 |
| mutational and transcriptional analysis of the type iii secretion system of bradyrhizobium japonicum. | sequencing the symbiotic region of bradyrhizobium japonicum revealed a gene cluster (tts) encoding a type iii secretion system (ttss) that is similar to those found in mesorhizobium loti maff303099 and rhizobium strain ngr234. in addition to genes that are likely to encode structural core components of the ttss, the cluster contains several open reading frames that are found exclusively in rhizobia or that are specific to b. japonicum. depending on the host, mutations within this cluster affecte ... | 2002 | 12481995 |
| 16s ribosomal dna-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by pcr and denaturing gradient gel electrophoresis. | the bacterial community in partially purified water, which is prepared by ion exchange from tap water and is used in pharmaceutical manufacturing processes, was analyzed by denaturing gradient gel electrophoresis (dgge). 16s ribosomal dna fragments, including v6, -7, and -8 regions, were amplified with universal primers and analyzed by dgge. the bacterial diversity in purified water determined by pcr-dgge banding patterns was significantly lower than that of other aquatic environments. the bacte ... | 2002 | 11823209 |
| bradyrhizobium sp. nodulating the mediterranean shrub spanish broom (spartium junceum l.). | the molecular diversity of 25 strains of rhizobia, isolated in sicily from root nodules of the mediterranean shrubby legume spanish broom (spartium junceum l.), is presented in relation to the known rhizobial reference strains. | 2002 | 11849323 |
| comparison of molecular and antibiotic resistance profile methods for the population analysis of bradyrhizobium spp. (tgx) isolates that nodulate the new tgx soybean cultivars in africa. | comparison of molecular and antibiotic resistance profile methods to identify an easy method that can differentiate between strains of introduced bradyrhizobium japonicum and the indigenous bradyrhizobium spp. (tgx) isolates which nodulate the newly developed tgx soybean cultivars in africa. | 2002 | 11849334 |
| crystallization and preliminary x-ray crystallographic analysis of malonamidase e2, an amidase signature family member. | malonamidase e2 from bradyrhizobium japonicum catalyzes the hydrolysis of malonamate. the enzyme belongs to an amidase signature family which has a highly conserved serine- and glycine-rich sequence over a stretch of approximately 45 amino acids. more than 100 known or predicted members belonging to this family, whose biological functions vary widely, can be identified in sequence databases. although urgently needed, no three-dimensional structure of any protein of this family is yet available. ... | 2002 | 11856856 |
| morphological changes of rhizobia in peat cultures. | morphological changes that take place in peat cultures of several species of rhizobia were examined. these changes seemed to be associated with enhanced survival of cells in peat and after inoculation onto plastic beads, which were used as a model system for seeds. cell wall changes, in which the periplasmic space appeared to be occluded with electron-dense material, were observed in rhizobium sp. strain su343 and bradyrhizobium lupini wu425 cells after 7 and 14 days in peat, respectively. nutri ... | 2002 | 11872451 |
| the aquatic budding bacterium blastobacter denitrificans is a nitrogen-fixing symbiont of aeschynomene indica. | blastobacter spp. are freshwater bacteria that form rosette structures by cellular attachment to a common base. comparative analyses of ribosomal 16s rrna gene and internally transcribed spacer region sequences indicated that b. denitrificans is a member of the alpha-subdivision of proteobacteria. among the alpha-proteobacteria, b. denitrificans was related to a cluster of genera, including rhodopseudomonas palustris, afipia felis, nitrobacter hamburgensis, and bradyrhizobium spp. although the p ... | 2002 | 11872460 |
| a two-component regulator mediates population-density-dependent expression of the bradyrhizobium japonicum nodulation genes. | bradyrhizobium japonicum nod gene expression was previously shown to be population density dependent. induction of the nod genes is highest at low culture density and repressed at high population densities. this repression involves both nola and nodd2 and is mediated by an extracellular factor found in b. japonicum conditioned medium. nola and nodd2 expression is maximal at high population densities. we demonstrate here that a response regulator, encoded by nwsb, is required for the full express ... | 2002 | 11872728 |
| sinorhizobium fredii hh103 has a truncated nolo gene due to a -1 frameshift mutation that is conserved among other geographically distant s. fredii strains. | strain svq121 is a mutant derivative of sinorhizobium fredii hh103 carrying a transposon tn5-lacz insertion into the nolo-coding region. sequence analysis of the wild-type gene revealed that it is homologous to that of rhizobium sp. ngr234, which is involved in the 3 (or 4)-o-carbamoylation of the nonreducing terminus of nod factors. downstream of nolo, as in rhizobium sp. ngr234, the noei gene responsible for methylation of the fucose moiety of nod factors was found. svq121 nod factors showed l ... | 2002 | 11878319 |
| species-specific inhibition of porphobilinogen synthase by 4-oxosebacic acid. | porphobilinogen synthase (pbgs) catalyzes the condensation of two molecules of 5-aminolevulinic acid (ala), an essential step in tetrapyrrole biosynthesis. 4-oxosebacic acid (4-osa) and 4,7-dioxosebacic acid (4,7-dosa) are bisubstrate reaction intermediate analogs for pbgs. we show that 4-osa is an active site-directed irreversible inhibitor for escherichia coli pbgs, whereas human, pea, pseudomonas aeruginosa, and bradyrhizobium japonicum pbgs are insensitive to inhibition by 4-osa. some varian ... | 2002 | 11909869 |
| isolation and characterization of the major nod factor of bradyrhizobium japonicum strain 532c. | bradyrhizobium japonicum 532c nodulates soybean effectively under cool canadian spring conditions and is used in canadian commercial inoculants. the major lipo-chitooligosaccharide (lco), bacteria-to-plant signal was characterized by hplc, fab-mass spectroscopy maldi-tof mass spectroscopy and revealed to be lco nod bj-v (c18:1, mefuc). this lco is produced by type i strains of b. japonicum and is therefore unlikely to account for this strains superior ability to nodulate soybean under canadian c ... | 2002 | 11911611 |
| analysis of bacteria contaminating ultrapure water in industrial systems. | bacterial populations inhabiting ultrapure water (upw) systems were investigated. the analyzed upw systems included pilot scale, bench scale, and full size upw plants employed in the semiconductor and other industries. bacteria present in the polishing loop of the upw systems were enumerated by both plate counts and epifluorescence microscopy. assessment of bacterial presence in upw by epifluorescence microscopy (cyanotolyl tetrazolium chloride [ctc] and dapi [4',6'-diamidino-2-phenylindole] sta ... | 2002 | 11916667 |
| bradyrhizobia from wild phaseolus, desmodium, and macroptilium species in northern mexico. | rrna genetic markers were analyzed in 97 isolates of nodule bacteria from six legume species in chihuahua, mexico. the most common genotypes were widely shared across host species and had 16s rrna sequences identical to those of strains from an eastern north american legume (amphicarpaea) that are closely related to bradyrhizobium elkanii. | 2002 | 11916730 |
| a comparative resonance raman analysis of heme-binding pas domains: heme iron coordination structures of the bjfixl, axpdea1, ecdos, and mtdos proteins. | the heme-pas is a specialized domain with which a broad class of signal-transducing heme proteins detect physiological heme ligands. such domains exhibit a wide range of ligand binding parameters, yet they are all expected to feature an alpha-beta heme binding fold and a predominantly hydrophobic heme distal pocket without a distal histidine. we have compared, for the first time, the resonance raman spectra of several heme-pass: the heme-binding domains of bradyrhizobium japonicum fixl, escheric ... | 2002 | 11939776 |
| influence of biosolids compost on the bradyrhizobial genotypes recovered from cowpea and soybean nodules. | the interaction of biosolids compost application to soil and bradyrhizobial genotypes recovered from nodules was examined. among 170 isolates, seven genotypes were recovered from soils receiving either no biosolids application or rates of 73 or 146 mg/ha for three successive years. with the exception of one genotype, the distribution of the bacterial genotypes recovered from nodules was interrelated with the level of biosolids. two of the genotypes nodulated both soybean and cowpea. because soyb ... | 2002 | 11976752 |
| isolation of plant-growth-promoting bacillus strains from soybean root nodules. | endophytic bacteria reside within plant tissues and have often been found to promote plant growth. fourteen strains of putative endophytic bacteria, not including endosymbiotic bradyrhizobium strains, were isolated from surface-sterilized soybean (glycine max. (l.) merr.) root nodules. these isolates were designated as non-bradyrhizobium endophytic bacteria (neb). three isolates (neb4, neb5, and neb17) were found to increase soybean weight when plants were co-inoculated with one of the isolates ... | 2002 | 11989767 |
| bacteriophytochrome controls photosystem synthesis in anoxygenic bacteria. | plants use a set of light sensors to control their growth and development in response to changes in ambient light. in particular, phytochromes exert their regulatory activity by switching between a biologically inactive red-light-absorbing form (pr) and an active far-red-light absorbing form (pfr). recently, biochemical and genetic studies have demonstrated the occurrence of phytochrome-like proteins in photosynthetic and non-photosynthetic bacteria--but little is known about their functions. he ... | 2002 | 12000965 |
| rhizobial position as a main determinant in the problem of competition for nodulation in soybean. | selected bradyrhizobium japonicum strains inoculated on soybean seeds often fail to occupy a significant proportion of nodules when a competitor rhizobial population is established in the soil. this competition problem could result from a genetic/ physiological advantage of the adapted soil population over the introduced inoculant or from a positional advantage, as the soil population already occupies the soil profile where the roots will penetrate, whereas the inoculant remains concentrated aro ... | 2002 | 12010128 |
| an inducible activator produced by a serratia proteamaculans strain and its soybean growth-promoting activity under greenhouse conditions. | serratia proteamaculans 1-102 (1-102) promotes soybean-bradyrhizobia nodulation and growth, but the mechanism is unknown. after adding isoflavonoid inducers to 1-102 culture, an active peak with a retention time of about 105 min in the hplc fractionation was isolated using a bioassay based on the stimulation of soybean seed germination. the plant growth-promoting activity of this material was compared with 1-102 culture (cells) and supernatant under greenhouse conditions. the activator was appli ... | 2002 | 12021297 |
| novel 2,4-dichlorophenoxyacetic acid degradation genes from oligotrophic bradyrhizobium sp. strain hw13 isolated from a pristine environment. | the tfd genes of ralstonia eutropha jmp134 are the only well-characterized set of genes responsible for 2,4-dichlorophenoxyacetic acid (2,4-d) degradation among 2,4-d-degrading bacteria. a new family of 2,4-d degradation genes, cadrabkc, was cloned and characterized from bradyrhizobium sp. strain hw13, a strain that was isolated from a buried hawaiian soil that has never experienced anthropogenic chemicals. the cadr gene was inferred to encode an arac/xyls type of transcriptional regulator from ... | 2002 | 11751829 |
| evidence that carbon dioxide enrichment alleviates ureide-induced decline of nodule nitrogenase activity. | the hypothesis that elevated [co(2)] alleviates ureide inhibition of n(2)-fixation was tested. short-term responses of the acetylene reduction assay (ara), ureide accumulation and total non-structural carbohydrate (tnc) levels were measured following addition of ureide to the nutrient solution of hydroponically grown soybean. the plants were exposed to ambient (360 micromol mol(-1)) or elevated (700 micromol mol(-1)) [co(2)]. addition of 5 and 10 mm ureide to the nutrient solution inhibited n(2) ... | 2003 | 12495923 |