Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| acute postoperative alcaligenes xylosoxidans endophthalmitis. | 2003 | 12972784 | |
| identification of the active site residues of pseudomonas aeruginosa protease iv. importance of enzyme activity in autoprocessing and activation. | protease iv is a lysine-specific endoprotease produced by pseudomonas aeruginosa whose activity has been correlated with corneal virulence. comparison of the protease iv amino acid sequence to other bacterial proteases suggested that amino acids his-72, asp-122, and ser-198 could form a catalytic triad that is critical for protease iv activity. to test this possibility, site-directed mutations by alanine substitution were introduced into six selected residues including the predicted triad and id ... | 2003 | 12419815 |
| characterization and pathogenic significance of vibrio vulnificus antigens preferentially expressed in septicemic patients. | many important virulence genes of pathogenic bacteria are preferentially expressed in vivo. we used the recently developed in vivo-induced antigen technology (iviat) to identify vibrio vulnificus genes induced in vivo. an expression library of v. vulnificus was screened by colony blot analysis by using pooled convalescent-phase serum that had been thoroughly adsorbed with in vitro-expressed v. vulnificus whole cells and lysates. twelve clones were selected, and the sequences of the insert dnas w ... | 2003 | 14500463 |
| isolation and molecular characterization of thiosulfate-oxidizing bacteria from an italian rice field soil. | in rice paddy soils an active cycling of sulfur compounds takes place. to elucidate the diversity of thiosulfate-oxidizing bacteria these organisms were enriched from bulk soil and rice roots by the most probable number method in liquid medium. from the mpn enrichment cultures 21 bacterial strains were isolated on solid mineral medium, and could be further shown to produce sulfate from thiosulfate. these strains were characterized by 16s rdna analyses. the isolates were affiliated to seven diffe ... | 2003 | 14529188 |
| pathophysiology and management of pulmonary infections in cystic fibrosis. | this comprehensive state of the art review summarizes the current published knowledge base regarding the pathophysiology and microbiology of pulmonary disease in cystic fibrosis (cf). the molecular basis of cf lung disease including the impact of defective cystic fibrosis transmembrane regulator (cftr) protein function on airway physiology, mucociliary clearance, and establishment of pseudomonas aeruginosa infection is described. an extensive review of the microbiology of cf lung disease with pa ... | 2003 | 14555458 |
| the microbiology of glue ear in australian aboriginal children. | to study the bacterial cultures of middle ear aspirates from 27 aboriginal children with otitis media with effusion. | 2003 | 14629496 |
| achromobacter insolitus sp. nov. and achromobacter spanius sp. nov., from human clinical samples. | a polyphasic taxonomic study (employing whole-cell protein and fatty acid analyses, 16s rdna sequencing, dna-dna hybridization, determination of dna g+c content, antibiotic susceptibility testing and extensive phenotypic characterization) was performed on 10 isolates that appeared to be related to alcaligenes faecalis. the isolates were recovered from diverse environments that included human clinical samples. 16s rdna sequence analysis indicated that these isolates belonged to the genus achromob ... | 2003 | 14657110 |
| kerstersia gyiorum gen. nov., sp. nov., a novel alcaligenes faecalis-like organism isolated from human clinical samples, and reclassification of alcaligenes denitrificans rüger and tan 1983 as achromobacter denitrificans comb. nov. | a polyphasic taxonomic study was performed on nine isolates recovered from various human clinical samples. phenotypically, these isolates resembled alcaligenes faecalis. whole-cell protein analysis distinguished two different species, and this was confirmed by dna-dna hybridizations. cellular fatty acid analysis and 16s rdna sequence analysis indicated that these isolates were related to the genera alcaligenes, bordetella, achromobacter and pigmentiphaga and belonged to the family alcaligenaceae ... | 2003 | 14657111 |
| antibiotic treatment of multidrug-resistant organisms in cystic fibrosis. | respiratory tract infection with eventual respiratory failure is the major cause of morbidity and mortality in cystic fibrosis (cf). infective exacerbations need to be treated promptly and effectively to minimize potentially accelerated attrition of lung function. the choice of antibiotic depends on in vitro sensitivity patterns. however, physicians treating patients with cf are increasingly faced with infection with multidrug-resistant isolates of pseudomonas aeruginosa. in addition, innately r ... | 2003 | 14719998 |
| cytoplasmic expression of the achromobacter xylosoxidans blue copper nitrite reductase in escherichia coli and characterisation of the recombinant protein. | the gene of the achromobacter xylosoxidans (dsm 2402) blue copper-containing nitrite reductase was amplified using the polymerase chain reaction. dna sequence analysis reveals that the amino acid sequence is identical to those of the gifu1051 and the ncimb11015 a. xylosoxidans nitrite reductases. the gene encoding the mature coding region for dsm 2402 nitrite reductase was cloned into a pet-vector, overexpressed in the cytoplasm of escherichia coli bl21(de3), and the expressed holoprotein was pu ... | 2003 | 14965775 |
| ph-profile crystal structure studies of c-terminal despentapeptide nitrite reductase from achromobacter cycloclastes. | crystal structures of c-terminal despentapeptide nitrite reductase (nirc-5) from achromobacter cycloclastes were determined from 1.9 to 2.3a at ph 5.0, 5.4, and 6.2. nirc-5, that has lost about 30% activity, is found to possess quite similar trimeric structures as the native enzyme. electron density and copper content measurements indicate that the activity loss is not caused by the release of type 2 copper (t2cu). ph-profile structural comparisons with native enzyme reveal that the t2cu active ... | 2004 | 15003518 |
| reductively activated nitrous oxide reductase reacts directly with substrate. | in the terminal step of bacterial denitrification, n2o is converted to n2 at the mu4-sulfide bridged tetranuclear cuz center of nitrous oxide reductase. the enzyme can be activated by reduced methyl viologen, with up to a 15-fold increase in specific activity. the reductively activated nitrous oxide reductase from achromobacter cycloclastes was isolated and characterized by visible absorption and epr spectroscopy, and both methods showed that the cuz center can attain a [4cu(i)] oxidation state. ... | 2004 | 15012115 |
| degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer. | to investigate the factors governing the adhesion and activity of the 2,4,6-tribromophenol (tbp) degrading bacterium achromobacter piechaudii tbpz-n61 on chalk from a contaminated aquifer. | 2004 | 15012824 |
| 1h, 13c, 15n backbone and sidechain resonance assignments of apo-nosl, a novel copper(i) binding protein from the nitrous oxide reductase gene cluster of achromobacter cycloclastes. | 2004 | 15014237 | |
| persistent colonization of nine cystic fibrosis patients with an achromobacter (alcaligenes) xylosoxidans clone. | the present study was conducted to investigate the increasing incidence of achromobacter (previously alcaligenes) xylosoxidans isolates being recovered from sputum samples of cystic fibrosis patients at a cystic fibrosis department for adults in athens, greece. during the 1-year study period, a total of 34 isolates were detected persistently in 9 of 71 cystic fibrosis patients. the isolates exhibited resistance to multiple antimicrobial agents. isolates that were recovered repeatedly from each p ... | 2004 | 15024624 |
| [nosocomial infections in a general surgical ward]. | according to the national nosocomial infection surveillance system we analysed the post-surgical nosocomial infections in a surgery ward of perugia university. between may 2000 and april 2001, 677 patients were enrolled mean age 51.5 years: 355 (52%) male, 462 (68%) asa score 1, "clean" surgery in 355 cases (52%), cephazolin prophylaxis in 256 (38%); 11 (2%) patients deceased perioperatively. a total of 37 nosocomial infections, in 33 patients, were detected: 18 pneumonia (48.6%), 10 surgical si ... | 2004 | 15032335 |
| omega-amino acid:pyruvate transaminase from alcaligenes denitrificans y2k-2: a new catalyst for kinetic resolution of beta-amino acids and amines. | alcaligenes denitrificans y2k-2 was obtained by selective enrichment followed by screening from soil samples, which showed omega-amino acid:pyruvate transaminase activity, to kinetically resolve aliphatic beta-amino acid, and the corresponding structural gene (apta) was cloned. the gene was functionally expressed in escherichia coli bl21 by using an isopropyl-beta-d-thiogalactopyranoside (iptg)-inducible pet expression system (9.6 u/mg), and the recombinant apta was purified to show a specific a ... | 2004 | 15066855 |
| pacemaker lead endocarditis caused by achromobacter xylosoxidans. | we report the case of a 35-yr-old patient who presented with high fever and chills. he had undergone a patch closure of the ventricular septal defect 18 yr before. one year later, a vvi pacemaker was implanted via the right subclavian vein because of complete heart block. nine years after that, a new vvi pacemaker with another right ventricular electrode was inserted controlaterally and the old pacing lead was abandoned. trans-thoracic and trans-esophageal echocardiogram identified the pacemaker ... | 2004 | 15082906 |
| dialysis-associated alcaligenes xylosoxidans peritonitis: a pediatric case. | 2004 | 15104341 | |
| amoebae-resisting bacteria isolated from human nasal swabs by amoebal coculture. | amoebae feed on bacteria, and few bacteria can resist their microbicidal ability. amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (arb), which may be human pathogens. to isolate new arb, we performed amoebal coculture from 444 nasal samples. we recovered 7 (1.6%) arb from 444 nasal swabs, including 4 new species provisionally named candidatus roseomonas massiliae, c. rhizobium massiliae, c. chryseobacterium massiliae, and c. amoebinatus massiliae. t ... | 2004 | 15109415 |
| cloning, overexpression, and characterization of a novel thermostable penicillin g acylase from achromobacter xylosoxidans: probing the molecular basis for its high thermostability. | the gene encoding a novel penicillin g acylase (pga), designated pgaw, was cloned from achromobacter xylosoxidans and overexpressed in escherichia coli. the pgaw gene contains an open reading frame of 2586 nucleotides. the deduced protein sequence encoded by pgaw has about 50% amino acid identity to several well-characterized pgas, including those of providencia rettgeri, kluyvera cryocrescens, and escherichia coli. biochemical studies showed that the optimal temperature for this novel pga (pga6 ... | 2004 | 15128530 |
| determination of esterolytic and lipolytic activities of lactic acid bacteria. | lactic acid bacteria (lab) are considered weakly lipolytic compared with many other groups of bacteria (e.g., pseudomonas, bacillus, and achromobacter). the esterolytic and lipolytic systems of dairy lab remain poorly characterized. esterases from lactic acid bacteria, yeasts, and pseudomonas organisms may be involved in the development of fruity flavors in foods, and pregastric lipase and esterases are essential for the development of typical flavor in italian cheese. microbial lipases and este ... | 2004 | 15156059 |
| nucleotide sequence and expression of the ncr nickel and cobalt resistance in hafnia alvei 5-5. | the structural genes for the nickel and cobalt resistance of the conjugative plasmid pejh 501 of hafnia alvei 5-5, contained on a sali-ecori fragment of 4.8 kb, were cloned and sequenced. the dna sequence included five genes in the following order: ncra, ncrb, ncrc, ncry, and ncrx. the predicted amino acid sequences of ncra were homologous to the amino acid sequences of nreb of achromobacter xylosoxidans 31a. expression of ncr with the t7 rna polymerase-promoter system allowed escherichia coli b ... | 2004 | 15179604 |
| structural investigations and identification of the extracellular bacteriolytic endopeptidase l1 from lysobacter sp. xl1. | the n-terminal amino acid sequence (23 amino acid residues) and the amino acid composition of the extracellular bacteriolytic enzyme l1 of 21 kd from the bacterium lysobacter sp. xl1 have been determined. the enzyme was hydrolyzed by trypsin, the resulting peptides were isolated, and their primary structures were determined. a high extent of homology (92%) of the n-terminal amino acid sequence and the primary structure of isolated peptides of the enzyme l1 (62 amino acid residues or 31% of prote ... | 2004 | 15193123 |
| achromobacter xylosoxidans isolated from the sputum of a patient with cystic fibrosis mutation i1234v with pseudomonas aeruginosa. | 2004 | 15195220 | |
| plant growth-promoting bacteria confer resistance in tomato plants to salt stress. | the object of the work is to evaluate whether rhizobacteria populating dry salty environments can increase resistance in tomato to salt stress. seven strains of plant growth-promoting bacteria that have 1-aminocyclopropane-1-carboxylate (acc) deaminase activity were isolated from soil samples taken from the arava region of southern israel. following growth of these seedlings in the presence of 43 mm nacl for 7 weeks, the bacterium that promoted growth to the greatest extent was selected for furt ... | 2004 | 15246071 |
| ial-chs (internal airlift loop--ceramic honeycomb supports) reactor used for biodegradation of 2,4-dichlorophenol and phenol. | the internal airlift loop reactor with ceramic honeycomb supports (ial-chs) was applied for biodegradation of 2,4-dichlorophenol (2,4-dcp) and phenol. a strain of dcp-degrading bacteria isolated from activated sludge, achromobacter sp., was rapidly immobilized onto the ceramic honeycomb supports. the immobilized cells effectively biodegraded 2,4-dcp alone and together with phenol in batch and continuous-flow experiments. for example, 2,4-dcp was biodegraded from an influent concentration of 50 m ... | 2004 | 15303748 |
| bacterial communities and enzyme activities of pahs polluted soils. | three soils (i.e. a belgian soil, b-bt, a german soil, g, and an italian agricultural soil, i-bt) with different properties and hydrocarbon-pollution history with regard to their potential to degrade phenanthrene were investigated. a chemical and microbiological evaluation of soils was done using measurements of routine chemical properties, bacterial counts and several enzyme activities. the three soils showed different levels of polycyclic aromatic hydrocarbons (pahs), being their contamination ... | 2004 | 15331267 |
| mesophilic aerobic degradation of a metal lubricant by a biological consortium. | the metal-forming industries require the use of greases to lubricate metal surfaces during manufacturing operations, and the residues of these lubricants must be removed prior to finishing processes to protect and improve the appearance of the final product. an aqueous, biological metal-cleaning process operating under mild conditions (ph 9, 42 degrees c) eliminates the use of environmentally unfriendly cleaning materials such as chlorinated solvents by employing microorganisms to degrade grease ... | 2004 | 15378296 |
| bacteremia caused by achromobacter and alcaligenes species in 46 patients with cancer (1989-2003). | achromobacter and alcaligenes are emerging infectious gram-negative bacterial species that can affect immunosuppressed patients. the authors sought to determine the incidence and characteristics of bloodstream infections caused by these organisms in patients with underlying malignancies. | 2004 | 15389476 |
| structure-based engineering of alcaligenes xylosoxidans copper-containing nitrite reductase enhances intermolecular electron transfer reaction with pseudoazurin. | the intermolecular electron transfer from achromobacter cycloclastes pseudoazurin (acpaz) to wild-type and mutant alcaligenes xylosoxidans nitrite reductases (axnirs) was investigated using steady-state kinetics and electrochemical methods. the affinity and the electron transfer reaction constant (k(et)) are considerably lower between acpaz and axnir (k(m) = 1.34 mm and k(et) = 0.87 x 10(5) m(-1) s(-1)) than between acpaz and its cognate nitrite reductase (acnir) (k(m) = 20 microm and k(et) = 7. ... | 2004 | 15475344 |
| quantification of denitrifying bacteria in soils by nirk gene targeted real-time pcr. | denitrification, the reduction of nitrate to nitrous oxide or dinitrogen, is the major biological mechanism by which fixed nitrogen returns to the atmosphere from soil and water. microorganisms capable of denitrification are widely distributed in the environment but little is known about their abundance since quantification is performed using fastidious and time-consuming mpn-based approaches. we used real-time pcr to quantify the denitrifying nitrite reductase gene (nirk), a key enzyme of the d ... | 2004 | 15488276 |
| protein sequence analysis, cloning, and expression of flammutoxin, a pore-forming cytolysin from flammulina velutipes. maturation of dimeric precursor to monomeric active form by carboxyl-terminal truncation. | flammutoxin (ftx), a 31-kda pore-forming cytolysin from flammulina velutipes, is specifically expressed during the fruiting body formation. we cloned and expressed the cdna encoding a 272-residue protein with an identical n-terminal sequence with that of ftx but failed to obtain hemolytically active protein. this, together with the presence of multiple ftx family proteins in the mushroom, prompted us to determine the complete primary structure of ftx by protein sequence analysis. the n-terminal ... | 2004 | 15489231 |
| the completely sequenced plasmid pest4011 contains a novel incp1 backbone and a catabolic transposon harboring tfd genes for 2,4-dichlorophenoxyacetic acid degradation. | the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacterium achromobacter xylosoxidans subsp. denitrificans strain est4002 contains plasmid pest4011. this plasmid ensures its host a stable 2,4-d(+) phenotype. we determined the complete 76,958-bp nucleotide sequence of pest4011. this plasmid is a deletion and duplication derivative of pd2m4, the 95-kb highly unstable laboratory ancestor of pest4011, and was self-generated during different laboratory manipulations performed to increas ... | 2004 | 15489427 |
| amino acid sequence of a thrombin like enzyme, elegaxobin ii, from the venom of trimeresurus elegans (sakishima-habu). | the amino acid sequence of a thrombin like enzyme , named elegaxobin ii, isolated from the venom of trimeresurus elegans (sakishima-habu) was determined by edman sequencing of the peptides which was derived from digests with cyanogen bromide, achromobacter protease i, trypsin, endoproteinase asp-n, and chymotrypsin. elegaxobin ii consisted of 233 amino acids and showed conservation of the catalytic amino acid residues (his(57), asp(102), and ser(195)) of chymotrypsin family serine protease in it ... | 2004 | 15500847 |
| pitfalls of polymyxin antimicrobial susceptibility testing of pseudomonas aeruginosa isolated from cystic fibrosis patients. | with their potent activity against gram-negative bacteria, the polymyxins are important alternative antibiotics for cystic fibrosis (cf) patients. a retrospective evaluation of polymyxin activity against 6001 pseudomonas aeruginosa, 150 achromobacter xylosoxidans and 506 stenotrophomonas maltophilia cf isolates was initiated. in addition, we looked at how polymyxin susceptibility testing was affected by the testing method (agar dilution versus microdilution), the agent (polymyxin e versus polymy ... | 2004 | 15509615 |
| characterization of two type 1 cu sites of hyphomicrobium denitrificans nitrite reductase: a new class of copper-containing nitrite reductases. | we report (1) the amino acid sequence of hyphomicrobium denitrificans nitrite reductase (hdnir), containing two type 1 cu sites and one type 2 cu site; (2) the expression and preparation of wild-type hdnir and two mutants replacing the cys ligand of each type 1 cu with ala; and (3) their spectroscopic and functional characterization. the open-reading frame of 50-kda hdnir is composed of the 15-kda n-terminal domain having a type 1 cu-binding motif like cupredoxins and the 35-kda c-terminal domai ... | 2004 | 15518568 |
| cyclo(l-leucyl-l-prolyl) produced by achromobacter xylosoxidans inhibits aflatoxin production by aspergillus parasiticus. | aflatoxins are potent carcinogenic and toxic substances that are produced primarily by aspergillus flavus and aspergillus parasiticus. we found that a bacterium remarkably inhibited production of norsolorinic acid, a precursor of aflatoxin, by a. parasiticus. this bacterium was identified as achromobacter xylosoxidans based on its 16s ribosomal dna sequence and was designated a. xylosoxidans nfri-a1. a. xylosoxidans strains commonly showed similar inhibition. the inhibitory substance(s) was excr ... | 2004 | 15574949 |
| images in cardiovascular medicine. rare form of right ventricular outflow tract obstruction. | 2004 | 15583085 | |
| [biotransformation of organic substances by an immobilized associative bacterial culture]. | bacterial strains were screened to transform to end-products (carbon monoxide and water) elevated concentrations of acetone, acidic acid, and ethanol in a biocatalyst with an immobilized bacterial association cultivated on solid foam polyvinyl acetate (fpva). the innocuous association amalgamated paracoccus denitrificans vkm v-1324, pseudomonas esterophilus vkm v-1736d and achromobacter parvulus vkm v-1541d. the biocatalyst was tested with the help of classic methods and equipment for microbes c ... | 2004 | 15605736 |
| technical aspects of gel-based proteomics designed for elucidating an aryl hydrocarbon receptor complex. | the identification of proteins by mass spectrometry has revolutionalized the basic method of identifying proteins constituting an intracellular unit or network for certain biological functions. the gel-based strategy following immunoprecipitation was applied to elucidating proteins associated with the aryl hydrocarbon receptor (ahr). two hundred femtomoles of ahr was recovered from approximately 2 x 10(7) hepg2 cells by immunoprecipitation and was sufficient for identification by peptide mass fi ... | 2004 | 15746886 |
| biodegradation of 2,4,6-tribromophenol during transport in fractured chalk. | the effect of physicochemical conditions (residence time, oxygen concentrations, and chalk characteristics) on the biodegradation of 2,4,6-tribromophenol (tbp) during transport was investigated in low-permeability fractured-chalk cores. long-term (approximately 600 d) biodegradation experiments were conducted in two cores (approximately 21 cm diameter, 31 and 44 cm long, respectively), intersected by a natural fracture. tbp was used as a model contaminant and as the sole carbon source for aerobi ... | 2005 | 15757335 |
| plant-microbe interactions: identification of epiphytic bacteria and their ability to alter leaf surface permeability. | bacteria were either isolated from leaf surfaces of hedera helix or obtained from a culture collection in order to analyse their effect on barrier properties of isolated hedera and prunus laurocerasus cuticles. on the basis of the 16s rdna sequences the genera of the six bacterial isolates from hedera were identified as pseudomonas sp., stenotrophomonas sp. and achromobacter. water permeability of cuticles isolated from h. helix was measured before and after inoculation with the six bacterial st ... | 2005 | 15819920 |
| [application of molecular systematics to study of bacterial cultures consuming volatile organic compounds]. | a range of species of four mixed bacterial cultures was studied by molecular systematics methods with the use of 16s rrna genes. the cultures had been developed for application in minireactors, to degrade volatile organic compounds (vocs): ethyl benzene, m-xylene, styrene, and o-xylene. a sample of 30 plasmid rdna clones was obtained for each of the mixed cultures. the clones were analyzed by rflp according to two restriction sites. major variants of the 16s-rdna sequences, corresponding to the ... | 2005 | 15859460 |
| pusillimonas noertemannii gen. nov., sp. nov., a new member of the family alcaligenaceae that degrades substituted salicylates. | the taxonomic position of a pseudomonas-like strain, designated bn9(t), was investigated. this strain had previously been isolated as a 5-aminosalicylate-degrading organism from a 6-aminonaphthalene-2-sulphonate-degrading mixed bacterial culture. previously, detection of ubiquinone q-8, a polyamine pattern with putrescine, spermidine and 2-hydroxyputrescine as the major polyamines, and partial 16s rrna gene sequencing had suggested that strain bn9(t) belongs to the 'betaproteobacteria'. this was ... | 2005 | 15879236 |
| dynamic kinetic resolution of amino acid amide catalyzed by d-aminopeptidase and alpha-amino-epsilon-caprolactam racemase. | amino acid amide racemizing activity was discovered in alpha-amino-epsilon-caprolactam (acl) racemase (ec 5. 1. 1. 15) from achromobacter obae. the enzymatic synthesis of d-alanine from l-alanine amide has been demonstrated by use of d-aminopeptidase (dap; ec 3. 4. 11. 19) from ochrobactrum anthropi c1-38 and acl racemase. the conversion of 45 mm l-alanine amide was carried out at 30 degrees c for 7 h; l-alanine amide was completely converted to d-alanine, and no l-alanine was detected. the resu ... | 2005 | 15913357 |
| hydrocarbon biodegradation in oxygen-limited sequential batch reactors by consortium from weathered, oil-contaminated soil. | we studied the use of sequential batch reactors under oxygen limitation to improve and maintain consortium ability to biodegrade hydrocarbons. air-agitated tubular reactors (2.5 l) were operated for 20 sequential 21-day cycles. maya crude oil-paraffin mixture (13,000 mg/l) was used as the sole carbon source. the reactors were inoculated with a consortium from the rhizosphere of cyperus laxus, a native plant that grows naturally in weathered, contaminated soil. oxygen limitation was induced in th ... | 2005 | 15920621 |
| high resolution structural studies of mutants provide insights into catalysis and electron transfer processes in copper nitrite reductase. | we present high-resolution crystal structures and functional analysis of t1cu centre mutants of nitrite reductase that perturb the redox potential and the cys130-his129 "hard-wired" bridge through which electron transfer to the catalytic t2cu centre occurs. these data provide insight into how activity can be altered through mutational manipulation of the electron delivery centre (t1cu). the alteration of cys to ala results in loss of t1cu and enzyme inactivation with azurin as electron donor des ... | 2005 | 15927201 |
| shared genotypes of achromobacter xylosoxidans strains isolated from patients at a cystic fibrosis rehabilitation center. | during a study examining transmission of pseudomonas aeruginosa among 76 cystic fibrosis patients in a rehabilitation center, where patients stay in close contact during prolonged periods, several clusters of patients carrying genotypically identical p. aeruginosa, as well as two clusters of 4 and 10 patients, respectively, colonized with genotypically identical achromobacter xylosoxidans strains, were discovered. | 2005 | 15956444 |
| a novel bacterium achromobacter xylosoxidans as a cause of liver abscess: three case reports. | achromobacter xylosoxidans is a microorganism that may cause opportunistic infections. we detected a. xylosoxidans in three of 46 patients with liver abscess. the clinicopathologic findings of the three patients were uniform with respect to a prior history of cholecystectomy, multi-lobulated 'coral-like' abscess under ct and epithelioid granulomas in the periphery of the abscess. achromobacter xylosoxidans is an unrecognized cause of liver abscess in humans. | 2005 | 15978693 |
| antimicrobial peptide therapeutics for cystic fibrosis. | greater than 90% of lung infections in cystic fibrosis (cf) patients are caused by pseudomonas aeruginosa, and the majority of these patients subsequently die from lung damage. current therapies are either targeted at reducing obstruction, reducing inflammation, or reducing infection. to identify potential therapeutic agents for the cf lung, 150 antimicrobial peptides consisting of three distinct structural classes were screened against mucoid and multidrug-resistant clinical isolates of p. aeru ... | 2005 | 15980369 |
| diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes. | seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera pseudaminobacter, achromobacter, brucella, and ochrobactrum. southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from plesiomonas sp. strain m6 and were all located on the chromosome. gene libraries were constructed from genomic dna of each of the 7 organophosphorus pesticide-degrading bacter ... | 2005 | 15980896 |
| ['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp]. | gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ... | 2005 | 15991478 |
| antibiotic resistance and antibacterial activity in heterotrophic bacteria of mineral water origin. | antibiotic resistance and antibacterial activity were determined on heterotrophic bacteria isolated from mineral waters. of the 120 isolates pseudomonas spp. (55.8%) was the predominant group followed by acinetobacter spp. (14.17%), flavobacterium spp. (10.83%), achromobacter spp. (10%), burkholderia cepacia (3.3%), agrobacterium/radiobacter (2.5%), moraxella spp. (1.7%), aeromonas hydrophila (1.7%). over 80% of the isolates were resistant to one or more antibiotics and the highest resistance wa ... | 2005 | 15993695 |
| cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from achromobacter xylosoxidans. | amidases are very important enzymes for industrial biocatalysis. we scored a novel amidase by screening the achromobacter xylosoxidans gene library with cephalosporin analogous amides. the gene coding for the enzyme, designated ana, was cloned, sequenced and overexpressed in escherichia coli. sequence analysis of ana showed it to be an amidase signature family member. interestingly, we noted that almost all ana homologous amidases are from human pathogens responsible for chronic lung infections. ... | 2005 | 16002239 |
| taking the fungal highway: mobilization of pollutant-degrading bacteria by fungi. | the capacity of fungi to serve as vectors for the dispersion of pollutant-degrading bacteria was analyzed in laboratory model systems mimicking water-saturated (agar surfaces) and unsaturated soil environments (glass-bead-filled columns). two common soil fungi (fusarium oxysporum and rhexocercosporidium sp.) forming hydrophilic and hydrophobic mycelia, respectively, and three polycyclic aromatic hydrocarbon degrading bacteria (achromobacter sp. sk1, mycobacterium frederiksbergense lb501tg, and s ... | 2005 | 16047804 |
| [use of 16s rrna gene sequencing for identification of "pseudomonas-like" isolates from sputum of patients with cystic fibrosis]. | since nonfermenting, gram negative bacilli recovered from patients with cystic fibrosis could be misidentified with phenotypic procedures, we used partial 16s ribosomal rna gene (16s gene) sequencing to identify these "pseudomonas-like" isolates. 473 isolates were recovered from 66 patients in 2003. sequencing was used to identify 29 (from 24 patients) of the 473 isolates, showing unclear results with routine tests. pcr with specific primers was carried out to amplify a 995 bp fragment, which wa ... | 2005 | 16081224 |
| clonal relatedness and conserved integron structures in epidemiologically unrelated pseudomonas aeruginosa strains producing the vim-1 metallo-{beta}-lactamase from different italian hospitals. | three epidemiologically independent pseudomonas aeruginosa isolates, representative of the first vim-1 metallo-beta-lactamase producers detected at three different hospitals in northern italy, were investigated to determine their genomic relatedness and to compare the structures of the genetic supports for the vim-1 determinants. the three isolates, all of serotype o11, appeared to be clonally related according to the results of genotyping by macrorestriction analysis of genomic dna by pulsed-fi ... | 2005 | 15616282 |
| advenella incenata gen. nov., sp. nov., a novel member of the alcaligenaceae, isolated from various clinical samples. | a polyphasic taxonomic study of 14 isolates recovered from various human and veterinary clinical samples was performed. phenotypically these isolates shared several characteristics with members of the alcaligenaceae and related genera. random amplified polymorphic dna fingerprinting and whole-cell protein analysis suggested the presence of multiple genomic groups, which was confirmed by dna-dna hybridization experiments. 16s rrna gene sequence analysis indicated that these isolates were related ... | 2005 | 15653883 |
| quantifying maintenance requirements from the steady-state operation of a two-phase partitioning bioscrubber. | an innovative method for directly and explicitly quantifying the maintenance energy requirements of pure cultures growing on volatile organic compound (voc) substrates in a two-phase partitioning bioscrubber is described. direct evidence of maintenance energy requirements of achromobacter xylosoxidans y234 is provided both through observed reductions in the macroscopic biomass-to-substrate yield with decreasing specific growth rates, but more remarkably through achievement of steady-state operat ... | 2005 | 15726578 |
| atomic resolution structures of resting-state, substrate- and product-complexed cu-nitrite reductase provide insight into catalytic mechanism. | copper-containing nitrite reductases catalyze the reduction of nitrite to nitric oxide (no), a key step in denitrification that results in the loss of terrestrial nitrogen to the atmosphere. they are found in a wide variety of denitrifying bacteria and fungi of different physiology from a range of soil and aquatic ecosystems. structural analysis of potential intermediates in the catalytic cycle is an important goal in understanding enzyme mechanism. using "crystal harvesting" and substrate-soaki ... | 2005 | 16093314 |
| [alcaligenes xylosoxidans--a pathogen of chronic ear infection]. | we discuss a case of a 48-year old man admitted to our department with symptoms of chronic ear infection, that didn't answer to typical conservative treatment. the culture of the discharge was positive for alcaligenes xylosoxidans. | 2005 | 16095102 |
| characteristics of achromobacter xylosoxidans bacteremia in northern taiwan. | there were 40 cases of achromobacter xylosoxidans bacteremia during a 6-year period in a medical center in northern taiwan. males outnumbered females (72.5% vs 27.5%). common underlying diseases and conditions in these 40 bacteremic patients were malignancies (57.5%), central venous catheter implants (55%), surgery (35%), neutropenia (30%) and use of steroids (25%). recurrent bacteremia occurred in 4 patients (10%), all of whom had a central venous catheter implant which was considered the most ... | 2005 | 16118676 |
| roles of trp144 and tyr203 in copper-containing nitrite reductase from achromobacter cycloclastes iam1013. | the roles of the trp144 and tyr203 residues near the type 1 cu site of achromobacter cycloclastes nitrite reductase (acnir) have been examined with mutants of acnir. tyr203 is located on the protein surface near the type 1 cu site of acnir, and trp144 is between the tyr203 and the type 1 cu center in acnir. single mutation of trp144 or tyr203 in acnir to leu resulted in decreased rate constants of intermolecular electron transfer from its cognate pseudoazurin (acpaz) (k(et)=1.9x10(5), 2.2x10(5), ... | 2005 | 16125674 |
| tetrathiobacter kashmirensis gen. nov., sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in jammu and kashmir, india. | twelve chemolithotrophic strains were isolated from temperate orchard soil on reduced sulfur compounds as energy and electron sources and characterized on the basis of their physiological properties and ability to oxidize various reduced sulfur compounds. the new isolates could oxidize tetrathionate as well as thiosulfate, and oxidation of the latter involved conversion of thiosulfate to tetrathionate followed by its accumulation and eventual oxidation to sulfate, manifested in the production of ... | 2005 | 16166666 |
| degradation of natural rubber by achromobacter sp. nrb and evaluation of culture conditions. | a natural rubber degrading candidate was isolated from a soil sample from aswan, egypt. the strain was able to grow on natural rubber as a sole source for carbon and energy. according to its degradation behavior, it grew adhesively and in direct contact with the rubber substrate and led to disintegration of the material during cultivation. furthermore, this strain was not able to form a clear zone (translucent halos) around bacterial colonies after cultivation on nr latex plates. taxonomic analy ... | 2005 | 16209096 |
| a case of epidemic keratoconjunctivitis complicated by alcaligenes xylosoxidans infection. | to report a case of epidemic keratoconjunctivitis complicated by alcaligenes xylosoxidans. | 2005 | 16209287 |
| imipenem-resistant achromobacter xylosoxidans carrying blavim-2-containing class 1 integron. | we characterized seven isolates of imipenem-resistant achromobacter xylosoxidans that were isolated from patients hospitalized in the intensive care unit at a tertiary hospital in korea during 2001 to 2003. from the analysis with an isoelectric focusing, polymerase chain reaction, and sequencing methods, all isolates were found to produce vim-2, oxa-30, and chromosomal ampc beta-lactamase with a pi of 8.4. they showed a similar antibiogram, which were resistant to all tested aminoglycosides as w ... | 2005 | 16260111 |
| bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil. | bacterial community dynamics and biodegradation processes were examined in a highly creosote-contaminated soil undergoing a range of laboratory-based bioremediation treatments. the dynamics of the eubacterial community, the number of heterotrophs and polycyclic aromatic hydrocarbon (pah) degraders, and the total petroleum hydrocarbon (tph) and pah concentrations were monitored during the bioremediation process. tph and pahs were significantly degraded in all treatments (72 to 79% and 83 to 87%, ... | 2005 | 16269736 |
| chronic granulomatous disease presenting with eosinophilic inflammation. | chronic granulomatous disease (cgd), a rare immunodeficiency, typically presents with recurrent infections caused by catalase-positive organisms. we report 2 patients with cgd who presented with eosinophilic inflammatory conditions recognized before the diagnosis of cgd. both patients had significant urologic disease. physicians should be aware of the association of cgd with eosinophilic inflammatory conditions. | 2005 | 16282946 |
| outbreak of long-term intravascular catheter-related bacteremia due to achromobacter xylosoxidans subspecies xylosoxidans in a hemodialysis unit. | achromobacter xylosoxidans is a rare cause of bacteremia. over a 2-week period, a. xylosoxidans subsp. xylosoxidans was isolated from blood cultures of four hemodialysis patients with long-term intravascular catheters. a culture from one atomizer that contained diluted 2.5% chlorhexidine, which had been used to disinfect the skin, yielded a. xylosoxidans subsp. xylosoxidans. no further cases were diagnosed once the use of this atomizer was discontinued. five outbreak-related strains from the fou ... | 2005 | 16283217 |
| crystal structure of c-terminal desundecapeptide nitrite reductase from achromobacter cycloclastes. | monoclinic crystal structure of c-terminal desundecapeptide nitrite reductase (nirc-11) from achromobacter cycloclastes was determined at 2.6a. nirc-11 exists as a loose trimer in the crystal. deletion of 11 residues eliminates all intersubunit hydrogen bonds mediated by the c-terminal tail. the rigid irregular coil 105-112, which constitutes part of the sidewall of the active site pocket, undergoes conformational changes and becomes highly flexible in nirc-11. correspondingly, the linker segmen ... | 2005 | 16293231 |
| antimicrobial susceptibility patterns of unusual nonfermentative gram-negative bacilli isolated from latin america: report from the sentry antimicrobial surveillance program (1997-2002). | the antimicrobial susceptibility of 176 unusual non-fermentative gram-negative bacilli (nf-gnb) collected from latin america region through the sentry program between 1997 and 2002 was evaluated by broth microdilution according to the national committee for clinical laboratory standards (nccls) recommendations. nearly 74% of the nf-bgn belonged to the following genera/species: burkholderia spp. (83), achromobacter spp. (25), ralstonia pickettii (16), alcaligenes spp. (12), and cryseobacterium sp ... | 2005 | 16302068 |
| achromobacter xylosoxidans endophthalmitis diagnosed by polymerase chain reaction and gene sequencing. | to report a patient with achromobacter xylosoxidansendophthalmitis that was diagnosed using polymerase chain reaction and gene sequencing. | 2005 | 16321892 |
| transient performance of a two-phase partitioning bioscrubber treating a benzene-contaminated gas stream. | the dynamic performance of a prototype, two-phase partitioning bioscrubber in response to fluctuating benzene waste gas feeds has been characterized through a series of spike, step, and shutdown-restart experiments. from stable operation at a nominal loading capacity of 62 +/- 6 g/(m3 h) and removal efficiencies of over 99%, the bioscrubber was subjected to influent benzene concentration step changes and spikes as high as 10- and 20-fold, respectively. the bioscrubber responded rapidly and effec ... | 2005 | 16323802 |
| vim-2 metallo-beta-lactamase in achromobacter xylosoxidans in europe. | 2005 | 16333608 | |
| crystallization and preliminary x-ray crystallographic analysis of pas factor from vibrio vulnificus. | plasmid achromobacter secretion (pas) factor is a putative secretion factor that induces the secretion of periplasmic proteins. pas factor from vibrio vulnificus was crystallized at 294 k by the hanging drop vapor-diffusion method. it was isolated as a monomer during the purification procedures. the native crystal belongs to the f222 space group with unit cell parameters a=56.1, b=74.4, c=80.0 a, a=b=g=90 degrees. the crystal was soaked in cryoprotectant containing 1 m nabr for 1 h for mad phasi ... | 2005 | 16404150 |
| molecular detection of catabolic genes for polycyclic aromatic hydrocarbons in the reed rhizosphere of sunchon bay. | this study focused on detecting catabolic genes for polycyclic aromatic hydrocarbons (pahs) distributed in the reed rhizosphere of sunchon bay, korea. these marsh and mud environments were severely affected by human activities, including agriculture and fisheries. our previous study on microbial roles in natural decontamination displayed the possibility that pah-degrading bacteria, such as achromobacter sp., alcaligenes sp., burkholderia sp. and pseudomonas sp. play an important decontamination ... | 2005 | 16410776 |
| [study on biodiversity of type i & ii polyketide synthesis genes positive microorganisms]. | some soil samples were collected from different places in yunnan provinces, china. 876 bacteria or actinomycete strains were isolated using glucose-peptone-yeast extract agar, starch-casein agar and glycerol-asparagine agar with these soil samples, of which about 100 strains belong to genus streptomyces and the others belong to rare actinomycetes or bacteria. with polyketide synthesis gene screening, 75 strains were picked up as type i & ii polyketide synthesis gene positive strains. then 10 str ... | 2005 | 16496684 |
| phosphonium ionic liquids for degradation of phenol in a two-phase partitioning bioreactor. | six ionic liquids (ils), which are organic salts that are liquid at room temperature, were tested for their biocompatibility with three xenobiotic-degrading bacteria, pseudomonas putida, achromobacter xylosoxidans, and sphingomonas aromaticivorans. of the 18 pairings, seven were found to demonstrate biocompatibility, with one il (trihexyl(tetradecyl)phosphonium bis(trifluoromethylsulfonyl) amide) being biocompatible with all three organisms. this il was then used in a two-phase partitioning bior ... | 2005 | 15549289 |
| degradation of estradiol and ethinyl estradiol by activated sludge and by a defined mixed culture. | the aerobic degradation of the natural hormone 17-beta-estradiol (e2) and the synthetic hormone 17-alpha-ethinyl estradiol (ee2) was investigated in batch experiments with activated sludge from a conventional and a membrane sewage treatment plant. e2 was converted to estrone (e1), the well known metabolite, and further completely transformed within 3 days. the turnover rates of e2 did not differ greatly between conventional and membrane activated sludge. ee2 was persistent in both sludges. by se ... | 2005 | 15290133 |
| assessing the impact of denitrifier-produced nitric oxide on other bacteria. | a series of experiments was undertaken to learn more about the impact on other bacteria of nitric oxide (no) produced during denitrification. the denitrifier rhodobacter sphaeroides 2.4.3 was chosen as a denitrifier for these experiments. to learn more about no production by this bacterium, no levels during denitrification were measured by using differential mass spectrometry. this revealed that no levels produced during nitrate respiration by this bacterium were in the low mum range. this conce ... | 2006 | 16517672 |
| growth in stewart's medium is a simple, rapid and inexpensive screening tool for the identification of burkholderia cepacia complex. | ninety-one percent of burkholderia cepacia complex reference strains (66 out of 72) displayed a yellow slope-green butt colour reaction after growth in stewart's medium indicating the oxidation of glucose and the absence of an arginine dihydrolase system. this same colour reaction was observed for burkholderia gladioli and several ralstonia species, but not for pseudomonas aeruginosa, stenotrophomonas, achromobacter, pandoraea and several other gram-negative non-fermenting bacilli. we therefore ... | 2006 | 16386966 |
| cleavage with phospholipase of the lipid anchor in the cell adhesion molecule, csa, from dictyostelium discoideum. | a cell adhesion molecule, 80-kda csa, is involved in edta-resistant cell contact at the aggregation stage of dictyostelium discoideum. a 31-kda csa was isolated from the 80-kda csa by treatment with achromobacter protease i. results from thin-layer chromatography and maldi-tof ms analysis indicated that the 31-kda csa contains ceramide as a component of glycosylphosphatidyl-inositol (gpi). comparison between the 80-kda csa and the 31-kda csa treated with phosphatidylinositol-specific phospholipa ... | 2006 | 16388974 |
| investigation of hospital-acquired infections due to achromobacter xylosoxidans in a tertiary care hospital in india. | 2006 | 16289456 | |
| transient performance of two-phase partitioning bioreactors treating a toluene contaminated gas stream. | two-phase partitioning bioreactors (tppbs) consist of a cell-containing aqueous phase and an immiscible organic phase that sequesters and delivers toxic substrates to cells based on equilibrium partitioning. the immiscible organic phase, which acts as a buffer for inhibitory substrate loadings, makes it possible for tppbs to handle high volatile organic compound (voc) loadings, and in this study the performance of liquid n-hexadecane and solid styrene butadiene (sb) polymer beads used as partiti ... | 2006 | 16607657 |
| effects of microbial and phosphate amendments on the bioavailability of lead (pb) in shooting range soil. | 2006 | 16652251 | |
| chronic infection with achromobacter xylosoxidans in cystic fibrosis patients; a retrospective case control study. | in cystic fibrosis (cf), chronic infection of the airways with achromobacter xylosoxidans have become more frequent. the pathogenic role of this is yet unclear. | 2006 | 16777495 |
| effectiveness of biodegradation of petroleum products by mixed bacterial populations in liquid medium at different ph values. | the possibility of using mineral oils as a carbon source by bacteria adapted to high oil concentrations was tested in liquid media with different ph values (ph = 5, 7 and 9). two types of inocula were tested: inoculum i consisted of selected strains used in the bioremediation of oil-contaminated soils and inoculum ii contained bacteria isolated from soil samples previously bioremediated at ph = 5, 7 and 9. biodegradation was observed in all the investigated media independently of initial ph valu ... | 2006 | 16878607 |
| insight into catalysis of nitrous oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from achromobacter cycloclastes. | the difficult chemistry of nitrous oxide (n2o) reduction to gaseous nitrogen (n2) in biology is catalysed by the novel micro4-sulphide-bridged tetranuclear cuz cluster of the n2o reductases (n2or). two spectroscopically distinct forms of this cluster have been identified as cuz and cuz*. we have obtained a 1.86 a resolution crystal structure of the pink-purple species of n2or from achromobacter cycloclastes (acn2or) isolated under aerobic conditions. this structure reveals a previously unobserve ... | 2006 | 16904686 |
| benzene vapor treatment using a two-phase partitioning bioscrubber: an improved steady-state protocol to enhance long-term operation. | the performance and stability of a two-phase partitioning bioscrubber (tppb) containing 33% (vol.) n-hexadecane as an immiscible phase was investigated during 30 days of continuous gaseous benzene treatment. elimination capacities of 141 +/- 12 g/m(3) h were achieved by achromobacter xylosoxidans y234 while maintaining >99% removal throughout. a new steady-state operating strategy that limits excessive biomass production by directing substrate consumption to maintenance energy has eliminated the ... | 2006 | 16941180 |
| pannonibacter phragmitetus, described from a hungarian soda lake in 2003, had been recognized several decades earlier from human blood cultures as achromobacter groups b and e. | we performed a polyphasic taxonomic study on isolates previously tentatively classified as achromobacter groups b and e in comparison with the type strain of pannonibacter phragmitetus, lmg 22736(t)=nctc 13350(t). comparative 16s rrna gene sequence analysis suggested that strains of achromobacter groups b and e belong to p. phragmitetus (similarity levels were higher than 99 %). dna-dna hybridization experiments and other genotypic and phenotypic analyses confirmed that the three taxa represent ... | 2006 | 17159003 |
| infection of a total hip prosthesis in a dog caused by achromobacter(alcaligenes) xylosoxidans. | a four-year-old male, neutered labrador retriever was presented with progressive left hindlimb lameness 10 months following total hip replacement. radiography revealed changes consistent with infection and culture of joint fluid from the left coxofemoral joint revealed achromobacter (alcaligenes) xylosoxidans. the prosthesis was removed. culture of the acetabular cup confirmed achromobacter xylosoxidans. achromobacter xylosoxidans is an uncommon but serious cause of nosocomial epidemics in hospi ... | 2006 | 17201829 |
| [isolation, identification and degradation characteristics of bisphenol a degrading strain]. | a bisphenol a(bpa) degrading strain b-16 was isolated from compost leachate of municipal solid waste. according to the morphological characteristics, physiological and biochemical, and sequence analysis of 16s rdna, the strain was identified preliminarily as achromobacter xylosoxidans. by tests in shaking flasks, the effects of the conditions of growth was studied, and it was determined that the optimum conditions were inoculum amount 0.6%, ph 7.0, and 30 degrees c. under these conditions, when ... | 2006 | 17256617 |
| veb-1 in achromobacter xylosoxidans from cystic fibrosis patient, france. | 2006 | 17283625 | |
| fluorescence in situ hybridization for rapid identification of achromobacter xylosoxidans and alcaligenes faecalis recovered from cystic fibrosis patients. | achromobacter xylosoxidans is frequently isolated from the respiratory secretions of cystic fibrosis (cf) patients, but identification with biochemical tests is unreliable. we describe fluorescence in situ hybridization assays for the rapid identification of achromobacter xylosoxidans and alcaligenes faecalis. both assays showed high sensitivities and high specificities with a collection of 155 nonfermenters from cf patients. | 2006 | 16954289 |
| plant growth promoting potential of free-living diazotrophs and other rhizobacteria isolated from northern indian soil. | the viable count of free-living diazotrophic bacteria in different crop rhizospheres varied from 1.11 x 10(4) to 8.5 x 10(5) cfu/g of soil. the majority of the diazotrophs phenotypically belong to either azotobacter chroococcum, non-a. chroococcum type and to a heterogenous group tentatively named putative nitrogen-fixing (pnf) bacteria. in this study, 25 isolates of the pnf group were screened for their multiple plant growth-promoting (pgp) traits and grouped into 5 pgp types. an isolate, pnf(1 ... | 2006 | 17004301 |
| ciprofloxacin-resistant gram-negative bacilli in the fecal microflora of children. | the extent to which antibiotic-resistant bacteria are excreted by humans who have not been exposed to antibiotics is not known. children, who rarely receive fluoroquinolones, provide opportunities to assess the frequency of fecal excretion by fluoroquinolone-naïve hosts of fluoroquinolone-resistant gram-negative bacilli. fresh nondiarrheal stools from children were processed by screening them on agar containing ciprofloxacin to recover ciprofloxacin-resistant gram-negative bacilli. resistant iso ... | 2006 | 17005812 |
| structural studies of apo nosl, an accessory protein of the nitrous oxide reductase system: insights from structural homology with merb, a mercury resistance protein. | the formation of the unique catalytic tetranuclear copper cluster (cu(z)) of nitrous oxide reductase, n(2)or, requires the coexpression of a multiprotein assembly apparatus encoded by the nosdfyl operon. nosl, one of the proteins encoded by this transcript, is a 20 kda lipoprotein of the periplasm that has been shown to bind copper(i), although its function has yet to be detemined. cu(i) exafs data collected on the holo protein demonstrated that features of the copper binding site are consistent ... | 2006 | 17014077 |
| construction of a leftover bath water model for microbial testing. | in this study, in order to construct a model of leftover bath water, we analyzed one hundred samples of used bath water samples which were provided by twenty-eight volunteer families. it appeared that the number of detected bacteria from such bath water was correlated closely with the number of bathers. moreover, the ph, acidity, chemical oxygen demand (cod), ion, protein content of the leftover bath water were measured. the number of bathers had no connection with the ph, acidity, cod, and ion ... | 2006 | 17017128 |