Publications

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porphyromonas gingivalis within placental villous mesenchyme and umbilical cord stroma is associated with adverse pregnancy outcome.intrauterine presence of porphyromonas gingivalis (pg), a common oral pathobiont, is implicated in preterm birth. our aim was to determine if the location of pg within placental and/or umbilical cord sections was associated with a specific delivery diagnosis at preterm delivery (histologic chorioamnionitis, chorioamnionitis with funisitis, preeclampsia, and preeclampsia with hellp-syndrome, small for gestational age). the prevalence and location of pg within archived placental and umbilical cord ...201626731111
infectious and coronary artery disease.atherosclerotic event is one of the most causes of death in the world. coronary artery disease (cad) is one manifestation of atherosclerosis. it is well-known that several risk factors, such as diabetes mellitus (dm), smoking, hypertension (htn), have effects on it. it is proposed that infection can lead to atherosclerosis or even make its process faster. here, we discuss about the effect of some of infectious agents on the atherosclerosis and cad.201627114736
optimized use of the maldi biotyper system and the filmarray bcid panel for direct identification of microbial pathogens from positive blood cultures.despite the current reliance on blood cultures (bcs), the diagnosis of bloodstream infections (bsis) can be sped up using new technologies performed directly on positive bc bottles. two methods (the maldi biotyper system and filmarray blood culture identification [bcid] panel) are potentially applicable. in this study, we performed a large-scale clinical evaluation (1,585 microorganisms from 1,394 bsi episodes) on the combined use of the maldi biotyper and filmarray bcid panel compared to a refe ...201626677254
the relationship between the immune system and oral manifestations of inflammatory bowel disease: a review.inflammatory bowel diseases (ibds) are chronic, relapsing inflammatory diseases characterized by exacerbations and remissions of the gastrointestinal tract, clinically manifested as crohn's disease and ulcerative colitis. the etiology of ibds is considered to be multi factorial, comprising environmental, immune, microbial and genetic factors. clinical signs may include abdominal pain, frequent bloody diarrheas, mucorrhea, vomiting, fever, fatigue or weight loss. changes in the oral cavity often ...201627833449
influence of highly-active antiretroviral therapy on the subgingival biofilm in hiv-infected patients.highly-active antiretroviral therapy (haart) has been associated with alterations in subgingival biofilm and periodontal disease. the purpose of the present study was to investigate the association between different haart regimens and the prevalence of periodontal pathogens in hiv-infected patients with chronic periodontitis.201625283543
microbial dynamics during conversion from supragingival to subgingival biofilms in an in vitro model.the development of dental caries and periodontal diseases result from distinct shifts in the microbiota of the tooth-associated biofilm. this in vitro study aimed to investigate changes in biofilm composition and structure, during the shift from a 'supragingival' aerobic profile to a 'subgingival' anaerobic profile. biofilms consisting of actinomyces oris, candida albicans, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar were aerobically grown in saliva ...201626033167
tlr4, nod1 and nod2 mediate immune recognition of putative newly identified periodontal pathogens.periodontitis is a polymicrobial inflammatory disease that results from the interaction between the oral microbiota and the host immunity. although the innate immune response is important for disease initiation and progression, the innate immune receptors that recognize both classical and putative periodontal pathogens that elicit an immune response have not been elucidated. by using the human oral microbe identification microarray (homim), we identified multiple predominant oral bacterial speci ...201626177212
the current weight of evidence of the microbiologic profile associated with peri-implantitis: a systematic review.there is currently no consensus regarding microorganisms that may be considered true peri-implant pathogens. therefore, the aim of this systematic review is to determine the weight of evidence for microorganisms related to peri-implantitis based on results of association studies.201627420109
first report of severe acute otitis media caused by campylobacter rectus and review of the literature.campylobacter rectus is a member of the human oral flora and is associated with periodontal disease. we report the first case of severe acute otitis media (aom) due to c. rectus in a previous healthy 15-year-old boy, which was confirmed by 16s ribosomal rna gene sequencing. c. rectus is a possible causative pathogen of aom.201627363873
microbial complexes levels in conventional and self-ligating brackets.the aims were to evaluate the levels of bacterial species in saliva and in situ and to assess whether the design of brackets influences the risk of developing periodontal disease.201627270902
does obesity influence the subgingival microbiota composition in periodontal health and disease?to evaluate whether obesity affects the subgingival microbial composition of patients with periodontal health or chronic periodontitis (cp).201627717180
longitudinal study on clinical and microbial analysis of periodontal status in pregnancy.this study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (fish) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. probing depth (pd), bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque sampl ...201627556678
bacterial load of periodontal pathogens among italian patients with chronic periodontitis: a comparative study of three different areas.the aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in italian patients affected by chronic periodontitis. the sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology sampled in the period 2013-2015; 1,323 patients were from northern italy, 317 from central italy and 122 from southern italy. samples for microbiological analysis were collected from ...201627469562
evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using pcr analysis: a microbiological pilot study.the use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. over the last few years, carvacrol and thymol (ct) have been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of ct on the red complex bacteria using polymerase chain reaction (pcr) for microbiological analysis. five patients with a diagnosis of chronic periodontitis in the age grou ...201627469559
oral microbiota, periodontal status, and osteoporosis in postmenopausal females.alterations in hormone levels during menopause decrease bone density and may worsen oral health, favoring the growth of periodontal pathogens, whose detection could improve the diagnosis of periodontitis. the aim of this study is to detect and quantify the main periodontal pathogens in the oral microbiota of postmenopausal females and to explore the relationship between clinical and periodontal parameters.201626469811
microbiologic and clinical findings of implants in healthy condition and with peri-implantitis.to compare implants in healthy conditions and implants with peri-implantitis with regard to their clinical parameters and the microbiologic composition at the peri-implant sulcus, inside the implant connection, and the gingival sulcus of neighboring teeth.201626252036
detection of subgingival periodontal pathogens--comparison of two sampling strategies.the aim of the study is to compare detection frequency of periodontal pathogens in patients with aggressive/severe chronic periodontitis using pooled plaque samples from the deepest pockets per quadrant/per sextant.201626193958
microbiota at teeth and implants in partially edentulous patients. a 10-year retrospective study.to determine the microbiota at implants and adjacent teeth 10 years after placement of implants with a sandblasted and acid-etched surface.201625827437
periodontal pathogens and risk of incident cancer in postmenopausal females: the buffalo osteoperio study.extraoral translocation of oral bacteria may contribute to associations between periodontal disease and cancer. the associations among the presence of three orange-complex periodontal pathogens (fusobacterium nucleatum, prevotella intermedia, and campylobacter rectus), two red-complex periodontal pathogens (porphyromonas gingivalis and tannerella forsythia), and cancer risk were investigated.201626513268
linkages between oral commensal bacteria and atherosclerotic plaques in coronary artery disease patients.coronary artery disease is an inflammatory disorder characterized by narrowing of coronary arteries due to atherosclerotic plaque formation. to date, the accumulated epidemiological evidence supports an association between oral bacterial diseases and coronary artery disease, but has failed to prove a causal link between the two. due to the recent surge in microbial identification and analyses techniques, a number of bacteria have been independently found in atherosclerotic plaque samples from co ...201628649401
microbiological, lipid and immunological profiles in children with gingivitis and type 1 diabetes mellitus.the aim of this study was to compare the prevalence of periodontal pathogens, systemic inflammatory mediators and lipid profiles in type 1 diabetes children (dm) with those observed in children without diabetes (ndm), both with gingivitis.201728403363
prevalence of periodontal pathogens among italian patients with chronic periodontitis: a retrospective study on 2992 patients.the aim of the present study was to evaluate the prevalence of some periodontal pathogens in italian adults with chronic periodontitis.201728757933
effects of photodynamic therapy in periodontal treatment: a randomized, controlled clinical trial.to evaluate the effects of photodynamic therapy (pdt) in the nonsurgical treatment of chronic periodontitis.201728667678
herpes simplex 1 and periopathogen role in peri-implantitis.the objectives of this study were to compare the qualitative and quantitative profiles of herpes simplex virus type i (hsv-1) in implant surfaces between participants with peri-implantitis (pi) and healthy peri-implant tissues and to quantitatively assess the relation between hsv-1 and periopathogens inside the microbiological profile associated with pi.201728512280
the oral microbiome and adverse pregnancy outcomes.significant evidence supports an association between periodontal pathogenic bacteria and preterm birth and preeclampsia. the virulence properties assigned to specific oral pathogenic bacteria, for example, fusobacterium nucleatum, porphyromonas gingivalis, filifactor alocis, campylobacter rectus, and others, render them as potential collaborators in adverse outcomes of pregnancy. several pathways have been suggested for this association: 1) hematogenous spread (bacteremia) of periodontal pathoge ...201728848365
maldi-tof ms contribution to the diagnosis of campylobacter rectus multiple skull base and brain abscesses.campylobacter rectus is rarely associated with invasive infection. both the isolation and the identification requirements of c. rectus are fastidious, probably contributing to an underestimation of its burden. we report the case of a 66-year-old man who developed several skull base and intracerebral abscesses after dental intervention. campylobacter rectus was isolated from the brain biopsy. within 45 minutes of reading the bacterial plate, the strain was accurately identified by maldi-tof ms. t ...201728736616
periodontal microbioma and rheumatoid arthritis: the role of porhyromonas gingivalis.rheumatoid arthritis is a disease, which can be described as an autoimmune response after molecular mimicry caused by infective agents. the current study aims at evaluating the correlation between rhematoid arthritis (ra) and periodontal disease (pd), with special attention to the microbioma detected in the gums. thirty-four patients with rd were recruited into the current study. among rheumatic parameters, rheumatoid factor (rf), anti-citrullinated protein antibody (ccp), hla-bdr1 and das28 wer ...201728691459
quantitative analysis of periodontal pathogens in periodontitis and gingivitis.periodontal tissues surround the teeth and provide their attachment. periodontal diseases include a mild and reversible form named gingivitis and periodontitis that is the main cause of tooth loss in adults. gingivitis, that affects gums and coronal junctional epithelium, as well as periodontitis, that is characterized by loss of connective tissue attachment, are caused by a persistent inflammatory response promoted by alteration of periodontal biofilm. the aim of the study was to test whether t ...201726511188
prevalence of eight putative periodontal pathogens in atherosclerotic plaque of coronary artery disease patients and comparing them with noncardiac subjects: a case-control study.the study was aimed to assess the prevalence of periodontal pathogens namely tannerella forsythia (t.f), campylobacter rectus (c.r), eikenella corrodens (e.c), porphyromonas gingivalis (p.g), treponema denticola (t.d), prevotella nigrescens (p.n) ,aggregatibacter actinomycetemcomitans (a.a), p.g (fi ma gene) and prevotella intermedia (p.i), in the subgingival and the atheromatous plaque of patients with coronary artery disease (cad), and comparing them with the noncardiac subjects thereby indica ...201726096116
periodontal pathogenic bacteria and ammp-8 findings depending on periodontal conditions of patients before and after liver transplantation.the aim of this single-center cross-sectional study was to detect the prevalence of selected periodontal pathogenic bacteria and active matrix metalloproteinase-8 (ammp-8) level in patients before (preltx) and after liver transplantation (postltx).201727074844
thoracic empyema caused by campylobacter rectus.we report a case of thoracic empyema caused by campylobacter rectus, an organism considered as a periodontal pathogen but rarely recovered from extraoral specimens. the patient fully recovered through drainage of purulent pleural fluid and administration of antibiotics. the present case illustrates that c. rectus can be a cause of not only periodontal disease but also pulmonary infection.201727681234
common polymorphisms in the ifi16 and aim2 genes are associated with periodontal disease.we investigated the single nucleotide polymorphism (snp) context of a previously identified periodontitis-associated locus and examined its association with microbial, biological and periodontal disease clinical parameters.201728387608
behavior of two tannerella forsythia strains and cell surface mutants in multispecies oral biofilms.as a member of subgingival multispecies biofilms, tannerella forsythia is commonly associated with periodontitis. the bacterium has a characteristic cell surface (s-) layer modified with a unique o-glycan. both the s-layer and the o-glycan were analyzed in this study for their role in biofilm formation by employing an in vitro multispecies biofilm model mimicking the situation in the oral cavity. different t. forsythia strains and mutants with characterized defects in cell surface composition we ...201728382776
effect of cigarette smoking on subgingival bacteria in healthy subjects and patients with chronic periodontitis.cigarette smoking is known to increase the risk of periodontal destruction and developing chronic periodontitis (cp). it is also reported to affect the subgingival bacterial profile among cp patients. however, studies on the effect of smoking on the bacterial profile among healthy subjects are still limited. therefore, the aim of this study was to investigate the impact of smoking on the subgingival bacterial profile in both healthy adults and cp patients.201728327165
dental plaque microbial profiles of children from khartoum, sudan, with congenital heart defects.few studies have focused on the bacterial species associated with the deterioration of the dental and gingival health of children with congenital heart defects (chd). the aims of this study were (1) to examine the dental plaque of children with chd in order to quantify bacterial load and altered bacterial composition compared with children without chd; and (2) to investigate the correlation between the level of caries and gingivitis and dental biofilm bacteria among those children. in this cross ...201728326155
quantitative analysis of periodontal pathogens using real-time polymerase chain reaction (pcr).the quantitative polymerase chain reaction (qpcr) is a variant of pcr aimed to detect and quantify a targeted dna molecule through the addition of probes labeled with fluorescent molecules that emit fluorescence within each amplification cycle, what results in fluorescence values proportional to the amount of accumulated pcr product. this chapter presents the detailed procedures for quantification of different periodontal pathogens (porphyromonas gingivalis, aggregatibacter actinomycetemcomitans ...201727924595
comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample?the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control).201727786617
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