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evaluation of a blood-free, charcoal-based, selective medium for the isolation of campylobacter organisms from feces.a blood-free, charcoal-based selective medium (csm) consisting of a columbia agar base, activated charcoal (4 g/liter), hematin (0.032 g/liter), sodium pyruvate (0.1 g/liter), cefoperazone (32 mg/liter), vancomycin (20 mg/liter), and cycloheximide (100 mg/liter) supported the growth of campylobacter jejuni and c. coli with colony counts equivalent to those obtained on antibiotic-free horse blood agar. csm was compared to skirrow medium (skm) for the recovery of c. jejuni and c. coli from stools ...19863958143
distribution and serotypes of campylobacter jejuni and campylobacter coli in enteric campylobacter strains isolated from children in the central african republic.one hundred eighty-five enteric campylobacter strains isolated from diarrheic or healthy children in bangui (central african republic) were studied to determine their species and serotypes. c. coli was identified in 38.9% of all strains and in 43.9% of strains from diarrheic children. by the hemagglutination technique for heat-stable antigens, 73.5% of the strains could be serotyped. of the typeable strains, 75% were distributed among 13 more frequent serotypes. c. coli serotype pen 37,56 was th ...19863958146
colonization of syrian hamsters with streptomycin resistant campylobacter jejuni.a streptomycin resistant campylobacter jejuni inoculated per os into two populations of syrian hamsters (one endemically harboring c. jejuni, the other free of c. jejuni) established chronic colonization of the organism in both groups. diet, steroid administration, age of hamsters or prior exposure to c. jejuni did not appreciably alter incidence of diarrhea or colonization of c. jejuni. the majority of hamsters sampled during the course of the experiment (1 to 22 weeks) shed streptomycin resist ...19863959531
[campylobacter jejuni. infections in northern norway in 1980-84]. 19863961747
campylobacter jejuni septicemia--epidemiology, clinical features and outcome.in 33 cases of campylobacter jejuni septicemia, the disease was more common at the extremes of age: infants made up a third of the reported cases while 24% of patients were older than 50 years. fever was noted in more than 80% of patients and chills in about a fourth. enteritis was present in 70% of cases, and the gastrointestinal tract was the principal source of septicemia. half of the patients did not have significant underlying disease but were at extremes of age, which may reflect relative ...19863962297
[suitability of various culture media for medium-term storage of campylobacter jejuni/coli strains]. 19863962367
campylobacter jejuni enteritis associated with raw goat's milk.during a three-week period in july 1983, six cases of campylobacter jejuni enteritis in king county, washington were associated with a dairy that produced raw goat's milk. four patients consumed the dairy's milk, and the other two patients comprised an employee of the dairy and her infant son. two case-control studies confirmed that, at the time the cases occurred, consumption of the dairy's milk was a risk factor for c. jejuni enteritis in king county. c. jejuni was isolated from the intestinal ...19873605046
isolation of campylobacter spp. from pigeon feces by a combined enrichment-filtration technique.a technique combining enrichment in preston enrichment broth and direct filtration onto chocolate agar was used to isolate campylobacter species from pigeon feces. campylobacter jejuni was isolated from 106 of 200 samples tested; 105 strains were isolated by enrichment-filtration, and 84 strains were isolated by direct plating. most of the strains grew after 48 h at 37 degrees c.19873606114
minicell formation by campylobacter jejuni.campylobacter jejuni sheds its flagella and varying proportions of the poles of the cell late in the growth cycle, resulting in the production of very small flagellated structures 0.1 to 0.3 microm in diameter. electron microscopy revealed that these structures were minicells possessing outer membrane, cytoplasmic membrane, flagellar basal complex, and polar membrane; nucleoplasms were not seen. the initial event in the formation of these minicells involved a constriction of the cytoplasmic memb ...19873607612
atypical campylobacters associated with gastroenteritis.nine strains of campylobacter species other than campylobacter jejuni, campylobacter coli, and campylobacter laridis were isolated from patients with acute diarrhea. all nine strains showed preferred growth at 37 degrees c under microaerophilic conditions. conventional microbiological tests and dna-dna dot blotting were used to identify these strains. three of the nine campylobacter strains hydrolyzed hippurate, reduced nitrate, produced catalase, were resistant to cephalothin, and were shown to ...19873611317
kinetics of anti-campylobacter jejuni monomeric and polymeric immunoglobulin a1 and a2 responses in serum during acute enteritis.the intensity and kinetics of the serum polymeric and monomeric immunoglobulin a1 (iga1) and iga2 antibody responses to campylobacter jejuni were analyzed. a rapid and marked serum iga antibody response involving both the monomeric and polymeric components of iga was observed after c. jejuni infections. iga antibodies reached a peak of activity in serum during week 2 after the first symptoms of enteritis, about 10 days before the peak of igg activity. polymeric iga accounted for most of the anti ...19873611318
[pharmacokinetic and clinical studies of cefuzonam in pediatrics].pharmacokinetic and clinical studies were conducted to evaluate cefuzonam (l-105, czon), a new cephem type antibiotic, in the pediatric field. a total of 9 pediatric patients (2-14 years) was treated with intravenous injection of czon: 4 cases with one shot of 20 mg/kg, 2 cases with one shot of 40 mg/kg and 3 cases with drip infusion over 1 hour of 40 mg/kg. czon concentrations in serum and the excretion in urine were determined. mean serum concentrations of czon after one shot intravenous injec ...19873613084
[a serogrouping scheme for campylobacter jejuni by the slide agglutination method with formation-treated antigens]. 19873613099
[campylobacter jejuni in acute infantile diarrhea]. 19873616245
tubulointerstitial nephritis as a complication of campylobacter jejuni enteritis. a case report.tubulointerstitial nephritis occurred as a complication of campylobacter jejuni enteritis in a previously healthy young man. campylobacter jejuni was isolated from the patient's faeces and nephritis was confirmed by renal biopsy.19873616508
campylobacter enteritis at a university: transmission from eating chicken and from cats.campylobacter jejuni is the most common enteric pathogen isolated from university and college students in the united states. during the fall and winter quarters of the 1983-1984 academic year, the authors conducted a case-control study at the university of georgia, athens, georgia, to identify risk factors for c. jejuni enteritis. students with diarrhea whose cultures yielded c. jejuni were compared with controls matched by age, sex, and residence. a total of 45 case-control pairs were interview ...19873618583
effect of clavulanic acid on susceptibility of campylobacter jejuni and campylobacter coli to eight beta-lactam antibiotics.the effect of clavulanic acid on the susceptibility of 32 strains of campylobacter jejuni and campylobacter coli to eight beta-lactam agents was studied. almost all strains tested became susceptible to amoxicillin and ticarcillin with 1 microgram of clavulanic acid per ml. this compound had little or no effect on susceptibility to penicillin g, cephalothin, cefamandole, and cefoxitin. clavulanic acid had a marginal effect on cefotaxime and moxalactam susceptibility.19873619428
[an investigation of the campylobacter jejuni carrier state in healthy children of kindergartens]. 19873621322
utilization of monoclonal antibodies to evaluate the involvement of campylobacter jejuni in proliferative ileitis in syrian hamsters (mesocricetis auratus).the role of campylobacter jejuni in the pathogenesis of proliferative ileitis in syrian hamsters was evaluated with monoclonal antibodies of different specificities. monoclonal antibodies were produced with two different specificities: one for all members of the genus campylobacter tested (antibody 8322-2e6) and one for c. jejuni and campylobacter coli (antibodies 841-2a11, 841-4c6, and 841-5b1). heal sections from healthy hamsters, from hamsters with naturally occurring proliferative ileitis, a ...19873623701
factors affecting production of coccoid forms by campylobacter jejuni on solid media during incubation.conditions influencing the conversion of oxygen into toxic derivatives in media were investigated for their effects on production of coccoid forms in cultures of the enteric pathogen campylobacter jejuni. compared with stored media, production of coccoid forms was less on freshly prepared media. whether freshly prepared or stored before use, brucella agar media produced the fewest coccoid forms under the test conditions. addition of supplements used as detoxifying agents minimized production of ...19873624106
campylobacter enteritis in childhood.bacteriological examinations of faecal samples, obtained from 321 infants and children with acute enteritis, were carried out in the pediatric clinic of the university of occupational and environmental health, japan from january 1983 to december 1985. campylobacter jejuni were isolated in 48 infants and children (15%), while salmonella species in 6 (1.9%), and enteropathogenic escherichia coli in 11 (3.4%). of 48 infants and children with campylobacter enteritis (c. enteritis), 20 cases (42%) we ...19873576011
decontamination of campylobacter jejuni on chicken drumsticks using chemicals and radiation.a trial was conducted to compare the efficacy of four disinfectants and radiation to reduce the level of c. jejuni contamination on poultry meat. two levels of each treatment were applied to chicken drumsticks inoculated with a known concentration of a strain of campylobacter jejuni, biotype 1, isolated from a human patient with diarrhea. radiation using a cobalt-60 source at a level of 0.5 kgy effected a 99% surface reduction in c. jejuni. with a mean initial surface contamination level of 1.1 ...19873576968
anterior uveitis associated with campylobacter jejuni infection. 19873572019
use of monoclonal antibodies in local passive immunization to prevent colonization of human teeth by streptococcus mutans.local passive immunization with monoclonal antibodies (mabs) raised against streptococcal antigen (sa) i/ii protects monkeys against colonization of teeth by streptococcus mutans and the subsequent development of dental caries. in this study we extended the preclinical experiments to human subjects. in the first study of eight healthy subjects, four had anti-sa i/ii mab (immunoglobulin g2a [igg2a]) and four had saline applied to their teeth on three occasions. a streptomycin-resistant s. mutans ...19873570463
intestinal mucus gel and secretory antibody are barriers to campylobacter jejuni adherence to int 407 cells.an in vitro mucus assay was developed to study the role of mucus gel and secretory immunoglobulin a (siga) in preventing attachment of campylobacter jejuni to int 407 cells. an overlay of rabbit small intestinal mucus was found to impede the attachment of c. jejuni to a monolayer of int 407 cells. mucus from rabbits previously colonized with c. jejuni was found to completely inhibit bacterial adherence to the underlying cells. anti-campylobacter siga was readily detected in mucus samples from pr ...19873570473
effect of medium supplements, illumination and superoxide dismutase on the production of coccoid forms of campylobacter jejuni atcc 29428.factors influencing the production of coccoid forms in cultures and suspensions of a strain of the enteric pathogen campylobacter jejuni during storage in air were investigated. addition of blood or a supplement containing ferrous sulphate, sodium metabisulphite and sodium pyruvate minimized conversion of rods to coccoid forms in cultures. exposure of cultures to light during storage in air increased the rate of production of coccoid forms. ultraviolet radiation was shown to effect the viability ...19873571033
a study of thermophilic campylobacters in a river system.fifteen kilometres of a river system traversing rural and urban areas and subject to sewage works effluent discharge was studied during a 12 1/2 month period. a total of 312 samples was collected from 12 sites at 14 d intervals and tested by a glass microfibre filtration method and a most probable number (mpn) method. campylobacters were found in 43% of samples by the filtration method and 21% by the mpn method. the lowest frequency of isolation and lowest counts (less than 10 campylobacters/100 ...19873571039
differentiation of campylobacter and campylobacter-like organisms by cellular fatty acid composition.the cellular fatty acid compositions of 368 strains of campylobacter species or campylobacter-like organisms were determined by gas-liquid chromatography. most of the strains (339) were placed in one of three groups based on differences in fatty acid profiles. group a contained campylobacter jejuni (97%) and most c. coli (83%) strains and was characterized by the presence of a 19-carbon cyclopropane fatty acid (19:0 cyc) and 3-hydroxytetradecanoic acid (3-oh-14:0). group b included all c. laridi ...19873571478
diarrheal morbidity and mortality in children in the central african republic.diarrheal morbidity and mortality in children less than 5 years old were studied in bangui, central african republic, by a cluster survey. we found a high prevalence of diarrheal disease with an estimated annual incidence of 7 episodes of diarrhea per child per year. the estimated annual mortality rate for children less than 5 years old was 28.6 per 1,000 and 85.8 per 1,000 for infants; 51.6% of deaths were reported to be associated with diarrhea. during the survey, stool specimens were collecte ...19873578657
erythromycin-resistant campylobacter infections in thailand.erythromycin therapy was compared with no treatment in a prospective trial of acute diarrheal disease among 100 infants in an orphanage in bangkok. within 24 h of the onset of diarrhea, 50 children received erythromycin ethylsuccinate (40 mg/kg per day) in four divided doses for 5 days. campylobacter jejuni isolated from 31, campylobacter coli isolated from 21, and shigella spp. isolated from 21 of 100 children were the most commonly recognized pathogens; use of a sensitive, nonselective method ...19873579261
comparative in vitro antibacterial activities of two new oral cephalosporins, ceftetrame (ro 19-5247) and cefetamet (ro 15-8074).the in vitro activities of two new oral cephalosporins, ceftetrame (ro 19-5247) and cefetamet (ro 15-8074), were tested against 990 clinical bacterial isolates in comparison with that of cephalexin. both compounds were more active than cephalexin against gram-negative bacteria, inhibiting most isolates of the family enterobacteriaceae at concentrations of less than or equal to 4 micrograms/ml, but were not active against acinetobacter species, most pseudomonas species, campylobacter jejuni, and ...19873579264
seasonal occurrence of campylobacter spp. in surface waters and their correlation with standard indicator bacteria.campylobacter jejuni, campylobacter coli, and a campylobacter-like organism were isolated from a number of natural water sources in central washington, including ponds, lakes, and small mountain streams at elevations ranging from 1,460 to 5,400 feet (ca. 445 to 1,646 m) above sea level. at the two sites where extensive sampling was done, the bacteria were recovered throughout the year. generally, the recovery rates were highest in the fall and winter months and lowest during the spring and summe ...19873579268
[campylobacter jejuni infection in children]. 19873580099
gastric lavage: a simple method to obtain iga-rich intestinal secretions from the rabbit.a lavage procedure was developed to obtain intestinal secretions from rabbits. the procedure facilitated the repeated monitoring of the intestinal iga immune response of these animals to enteric infection with campylobacter jejuni. this non-invasive technique was easily performed, reproducible and yielded consistent levels of iga from rabbit intestinal secretions. it is anticipated that this procedure will aid in the study of the intestinal immune response of rabbits to other enteric pathogens.19873584997
in-vitro and in-vivo studies of a cytotoxin from campylobacter jejuni.studies were performed on a cytotoxin (ct) from human strains of campylobacter jejuni isolated in malaysia. ct was detected by cytopathic effect (cpe) on hela cells at titres from 8 to 32, in culture filtrates from 14 (48%) of 29 human isolates. the cpe correlated well with a quantitative 51cr-release assay where a specific release of 54-68% was noted. ct production was lost after 5-7 subcultures. ct activity was also detected in 5 (26%) of 19 faecal filtrates from which ct-producing isolates we ...19873585956
the mechanism of protection of infant mice from intestinal colonisation with campylobacter jejuni.balb/c mice, vaccinated intraperitoneally with a heat-killed (62 degrees c) suspension of campylobacter jejuni before mating, completely protect c. 90% of their own infants from intestinal colonisation. this protection has now been investigated further in fostering experiments. fostering by vaccinated dams within the first 24 h of life prevented intestinal colonisation in 50% of infants from non-vaccinated dams, and reduced colonisation in a further 25%. infants from vaccinated dams, even if all ...19873585966
pediatric gastroenteritis in primary care and in hospitalized patients.one-hundred and fifty-seven consecutive children below seven years of age (primary care n = 48, hospitalized patients n = 109) with acute gastroenteritis of assumed infectious origin were studied. rotavirus was demonstrated by electron microscopy of faeces in 44% of all patients. the occurrence of rotavirus among patients in primary care, 15%, was significantly lower than among hospitalized patients, 57% (p less than 0.01). adenovirus was isolated in six per cent and enterovirus in two per cent ...19873589234
[the epidemiology of campylobacter jejuni and campylobacter coli in the fattening chicken]. 19873589638
clinical impact of stool cultures for campylobacter in adults with acute or chronic diarrhea.campylobacter jejuni has emerged as a frequent cause of diarrhea. during a 12-month period at the houston veterans administration medical center, we isolated c jejuni from 3.4% of the 290 stool cultures from patients with diarrhea. this compared to an isolation rate of 4.1% for salmonella and 3.1% for shigella. during the same period, 17 additional cases of campylobacter-associated diarrhea were identified in adults at the two other baylor college of medicine teaching hospitals. we correlated th ...19873589763
serum agglutinin titers against somatic and flagellar antigens of campylobacter jejuni and isolation of campylobacter spp in chickens.from june 1983 to june 1984, two hundred twenty-five 3- to 30-month-old chickens (hens) on 10 different farms were examined for campylobacter spp. watering trays and fly vectors also were examined for campylobacter spp on 6 of the 10 farms. campylobacter jejuni was isolated from fecal specimens from 64 hens (28.4%), c coli was isolated from 6 hens (2.7%), and c laridis was isolated from 9 hens (4%). the isolation rate of c jejuni was 6.7% to 46.7% for 9 of the 10 farms. on 2 farms, agglutinin ti ...19873592379
comparative in vitro activity of the new oral cephalosporin bmy-28100.using a broth microdilution method, the in vitro activity of bmy-28100 against 365 clinical strains of commonly isolated bacteria was determined. bmy-28100 showed good activity against streptococci, methicillin-susceptible staphylococci, salmonella spp., shigella spp., and beta-lactamase producing branhamella catarrhalis and haemophilus influenzae. against susceptible strains of these organisms, bmy-28100 showed activity comparable to that of penicillin g, ampicillin, co-trimoxazole, erythromyci ...19873595580
a blood-free medium for isolation of thermophilic campylobacter species.a blood-free medium for the recovery of thermophilic campylobacter was compared with butzler medium virion during a one-year study using 2,893 human feces samples. ninety campylobacter strains (3.1%) were isolated after incubation for 48 h at 42 degrees c in a candle jar atmosphere. three strains of campylobacter jejuni were isolated on the blood-free medium only and one on butzler medium virion only. fecal flora was equally well inhibited on both media except for gram-positive organisms, which ...19873595583
campylobacter jejuni in dairy cows and raw milk.twelve herds of dairy cows were examined by rectal swabbing for the presence of campylobacter jejuni. ten herds were positive with the incidence of colonized animals ranging from 10 to 72% of those tested. with the exception of the two negative herds where mains water only was consumed, all animals drank from rivers or streams when grazing. there was no relationship between total and coliform counts and the presence of c. jejuni in raw milk. however, milk from one farm that consistently gave pos ...19873595744
efficacy of some methods and media for detecting and enumerating campylobacter jejuni in frozen chicken meat.four of five strains of campylobacter jejuni survived in chicken meat stored at -18 degrees c for 12 months. direct plating of samples was superior to the most probable number technique for enumerating c. jejuni. enrichment culture using the doyle & roman enrichment method resulted in the highest rates of detection. packaging under an atmosphere of co2 did not substantially influence survival.19873597202
[a waterborne outbreak of campylobacter jejuni infection]. 19873563986
[experimental animal studies with campylobacter jejuni and campylobacter coli in chickens]. 19873566434
a study of factors affecting the sensitivity of the passive haemagglutination method for serotyping campylobacter jejuni and campylobacter coli and recommendations for a more rapid procedure.factors affecting the sensitivity of the passive haemagglutination method for serotyping campylobacters have been studied. the concentration of red blood cells during the haemagglutination stage of the procedure markedly affected the titer obtained. an increase in concentration of red blood cells resulted in a lower titer, with titers being inversely proportional to red blood cell concentration. no differences in titer were observed when erythrocytes were sensitized at a range of ph values betwe ...19873567742
[infectious diarrhea in children. results of a prospective study concerning various epidemiological and clinical aspects of infectious diarrhea in children].over a 3-year period (from october 1981 to september 1984) 352 pediatric patients were treated for infectious diarrhea at the division for pediatric infectious diseases, department of pediatrics, university of berne, switzerland. routine diagnostic tests on stool samples included cultures for salmonella, shigella, campylobacter and yersinia, and enzyme-linked immunosorbent assay (rotazyme) for rotaviruses. in selected cases special tests for parasitic pathogens were also performed. the patients ...19873554501
current problems in antimicrobial therapy for bacterial enteric infection.trimethoprim/sulfamethoxazole is currently considered the treatment of choice for shigellosis and severe travelers' diarrhea. the problem with this combination regimen is inactivity against campylobacter jejuni strains and other bacterial enteropathogens showing in vitro resistance to the drug. resistance to trimethoprim/sulfamethoxazole among enteric pathogens has occurred frequently in certain areas of the world. a study of the in vitro susceptibility of enteric bacterial pathogens isolated fr ...19873555056
clinical efficacy of ciprofloxacin compared with placebo in bacterial diarrhea.in a double-blind, randomized trial, 85 adult patients with acute diarrhea (more than three watery stools per day) received either 500 mg of ciprofloxacin twice daily or placebo for five days. seventy-six patients were evaluated, 38 patients in the ciprofloxacin group (16 with salmonella species, 19 with campylobacter jejuni, and three with shigella species) and 38 patients in the placebo group (21 with salmonella species, 11 with c. jejuni, and six with shigella species). the duration of fever ...19873555057
causes of death in diarrhoeal diseases after rehydration therapy: an autopsy study of 140 patients in bangladesh.the cause of death (besides dehydration) for 140 diarrhoeal patients who died in hospital following rehydration was determined by autopsy examination. children under 5 years comprised 74% of the patients. diarrhoeal pathogens were identified as shigella spp. in 27%, enterotoxigenic escherichia coli in 17%, entamoeba histolytica in 16%, campylobacter jejuni in 12%, salmonella spp. in 4%, vibrio cholerae in 4%, and giardia lambliain 4% of cases. the most frequent underlying causes of death were co ...19873499247
[campylobacter jejuni in food originating from poultry]. 19873505671
comparative in-vitro activity of a-56268 (te-031), a new macrolide antibiotic.the comparative in-vitro activity of a-56268 (te-031), a new semisynthetic macrolide antibiotic, was assessed against approximately 400 bacterial isolates. the new drug demonstrated excellent activity against penicillin-susceptible streptococci (mic90s less than or equal to 0.06 mg/l) and methicillin-susceptible staphylococci (mic90 = 0.25 mg/l). among other gram-positive organisms tested, a significant number were resistant to a-56268 as well as to erythromycin and clindamycin. a-56268 was at l ...19872962974
gastrointestinal zoonoses.infectious gastrointestinal diseases affect man and animals throughout the world. certain etiologic agents (for example, salmonella spp., campylobacter jejuni, yersinia enterocolitica, cryptosporidia, strongyloides stercoralis, echinococcus granulosa) seem to have the potential to be transmitted from pets to people, causing severe disease in the latter. other agents seem unlikely to be transmitted but may have the potential to be zoonoses. this article discusses proved, suspected, and possible z ...19873551302
susceptibility of campylobacter jejuni and yersinia enterocolitica to uv radiation.two enteric pathogens, campylobacter jejuni and yersinia enterocolitica serogroup o:3, together with escherichia coli, were investigated for susceptibility to uv radiation at 254 nm. the uv dose required for a 3-log reduction (99.9% inactivation) of c. jejuni, y. enterocolitica, and e. coli was 1.8, 2.7, and 5.0 mws/cm2, respectively. using e. coli as the basis for comparison, it appears that c. jejuni and y. enterocolitica serogroup o:3 are more sensitive to uv than many of the pathogens associ ...19873551844
campylobacter jejuni enteritis in a premature neonate.we have described the first case of campylobacter jejuni enteritis in a premature neonate, whose clinical course was characterized by a self-limited bloody diarrhea accompanied by metabolic acidosis and poor weight gain. the importance of this organism in causing preterm labor in mothers and enteritis in premature neonates awaits systematic study.19873423907
studies on intestinal mucosal immunity in mice orally immunized with killed campylobacter jejuni. 19873425466
[gastroenteritis caused by campylobacter jejuni in childhood. study of 100 cases].authors report results of the study on a series of 100 pediatrics patients with campylobacter gastroenteritis whose ages were between 1 month and 14 years. incidence of campylobacter jejuni isolation among 2,733 samples of diarrheal stools, examined during a period of 3 years, was 146 cases (5.03%). several epidemiological, clinical and therapeutic aspects of the infection are analysed. campylobacter jejuni is an important enteric pathogen in infancy, and clinical symptoms due to infection are d ...19873426015
yoghurt: an unlikely source of campylobacter jejuni/coli.knowledge of the relative insensitivity of campylobacter jejuni to moderately acid environments prompted us to study its survival in different batches of yoghurt of ph range 4.2-5.3 and the role of organic or inorganic acid in the die-off of this pathogen. none of the 11 strains of c. jejuni or c. coli survived more than 25 min in yoghurt. suspecting that this rapid die-off cannot be accounted for by the ph of the yoghurt we compared the survival rates of c. jejuni in milk, whose ph had been adj ...19873429355
laboratory and clinical evaluation of isolation media for campylobacter jejuni.six selective isolation media were evaluated for their ability to support the growth of campylobacter jejuni. colony counts of 70 isolated strains of c. jejuni and recovery studies on these strains in simulated positive feces samples demonstrated that bolton and hutchinson' charcoal, cefoperazone, deoxycholate agar and karmali's charcoal-based selective medium produced the highest recovery rates with the greatest suppression of other fecal flora. c. jejuni colonies were more easily recognized on ...19873429621
dna probes for identification of tetracycline resistance genes in campylobacter species isolated from swine and cattle.tetracycline-resistant strains of campylobacter jejuni and campylobacter coli from swine and cattle colons were isolated and characterized by hybridization with dna probes. a probe consisting of the 1.8-kilobase (kb) hincii fragment from pua466 was highly specific for the detection of tetracycline resistance (tcr) in c. jejuni and c. coli. the 5-kb tetm dna probe from streptococcus agalactiae plasmid pji3 which has homology with the 1.8-kb hincii fragment from pua466 could also be used to detect ...19873435114
susceptibility of campylobacter species to metronidazole, its bioactive metabolites and tinidazole.the susceptibilities of 115 campylobacters, belonging to five species, to metronidazole, its principal oxidative metabolites and tinidazole were determined by an agar dilution method. the activities of metronidazole and tinidazole were comparable. the hydroxy metabolite was more active than the parent drug against most species, particularly campylobacter jejuni and campylobacter pyloridis. although the acid derivative was less active against c. jejuni and campylobacter coli, it demonstrated an e ...19873436679
antibody response in patients infected with campylobacter jejuni/coli assayed with a complement fixation test and a digelisa method.two methods for the detection of antibodies against campylobacter jejuni/coli, a complement fixation test (cft) and a dig-elisa method, were tested against a panel of single sera from 36 coproculture positive patients. twenty-five were positive in cft compared to 31 in the other system. twenty-three out of 25 cft positive sera were positive in the elisa method. seven sera were positive only for iga antibodies thus negative in the cft. cft may be useful for the detection of igg/igm antibodies aga ...19873438028
serogroups and biotypes of human strains of campylobacter jejuni and campylobacter coli isolated in france.the lior schemes were used for biotyping and serogrouping campylobacter strains isolated during a three year period in bordeaux, france. of the 226 strains tested, 176 were campylobacter jejuni and 50 campylobacter coli. campylobacter jejuni biotype i was the most prevalent (48.2%). biotypes iii and iv of campylobacter jejuni were rare (3.9% and 1.3% respectively). serogroup 4 (17.7%) was the most common serogroup followed by serogroups 46 (11.0%), 29 (10.1%), 9 and 36 (7.9%). eight serogroups c ...19873440456
frequency of campylobacter jejuni and other agents of acute diarrhea in venezuelan children. 19873442715
serological investigations on the cause of diarrhoea on the cape verde islands.in the serological tests 45.9% of all samples reacted positive against rota virus. 20.8% of samples revealed entamoeba histolytica positive sera, 25% shigella flexneri positive sera, 12.3% shigella sonnei and 13% shigella dysenteriae positive sera. campylobacter jejuni antibodies were found in 19% of samples, whereas 6.3% of sera reacted positive against yersinia enterocolitica type 3 and 4.8% against type 9.19873443746
serological investigations for the cause of diarrhoea among patients and personnel in the minna and abeokuta hospitals/nigeria.188 blood samples were investigated for antibodies against bacteria causing diarrhoea in two hospitals in minna and abeokuta in nigeria. antibodies were found against entamoeba histolytica, yersinia enterocolitica, campylobacter jejuni, salmonella typhi, salmonella paratyphi c, shigella dysenteriae, shigella sonnei, shigella boydii, shigella flexneri and rotavirus.19873443752
[survival of campylobacter jejuni in water with added potassium nitrate]. 19873445090
[intestinal infections of campylobacter jejuni etiology in infants in the first trimester of life]. 19873445610
campylobacter jejuni infection in a general hospital. 19873446687
[effects of disinfectants on campylobacter jejuni].the effect of three disinfectants on campylobacter jejuni strains isolated from poultry feces was investigated. two-fold dilutions of ethyl alcohol, formalin and benzalkonium chloride were contacted with bacterial suspension in 1 to 60 minutes. seventy per cent of ethyl alcohol and 2.5 per cent of formalin killed all bacteria within one minute. benzalkonium chloride (1:50.000) was effective to all bacteria within 5 minutes. under the conditions of this study, the recommended standard concentrati ...19873447021
prevalence of campylobacter jejuni in eggs and poultry meat in new york state.the presence of campylobacter jejuni was tested for but not isolated from any of 276 eggs sampled from 23 egg farms in new york state. the presence of c. jejuni was evaluated in broilers, kosher broilers, spent layers, peking ducks, and turkeys. four of five poultry dressing plants tested showed positive growth of c. jejuni on the 25-carcass samples at various stages of processing. twenty to 100% of live birds sampled contained c. jejuni on the skin but 90 to 100% were contaminated after scaldin ...19873447135
childhood enteritis: a community study.the population aged 0-12 years living in the area of an italian local health unit (about 8,800 children) was studied for one year in order to estimate the overall incidence rate of enteritis and the incidence rates of diarrheas from bacterial agents. all children complaining of acute diarrhea and seen by the pediatric practices of the study area were recorded by date, age, and sex; the microbiological study of stools was performed for a sample of patients. all hospitalized cases of childhood ent ...19873449099
[studies on the adhesion of campylobacter jejuni/coli to hela cells]. 19873449423
antimicrobial susceptibilities of diarrhoeagenic campylobacter jejuni and yersinia enterocolitica isolated in nigeria. 19873450406
campylobacter jejuni and yersinia enterocolitica in urban and rural nigerian school children. 19873450725
protection against campylobacter jejuni infection in suckling mice by anti-flagellar antibody.we obtained two monoclonal antibodies of igm class and iga class of immunoglobulin prepared from mouse spleen cells immunized with crude flagellar preparation, and a polyclonal antibody raised against purified flagellin monomer of campylobacter jejuni in a rabbit. the specificity of the reaction of these antibodies for flagellar filament was confirmed by western blotting and by immunoelectron microscopy. these antibodies caused agglutination of the bacteria and inhibited the motility of the bact ...19873451028
[effect of the components of preservative mixtures on the growth of campylobacter jejuni]. 19873451353
[bacteriostatic and bactericidal activity, resistance and tolerance of 16 strains of thermophilic campylobacter to 12 antibiotic drugs].the majority of gastrointestinal infections due to "thermophilic" campylobacter is self limiting and does not need antibiotic treatment. anyhow there are some serious cases (sepsis, persistent and relapsing gastroenteritis, severe immunodeficient patients) which require appropriate therapy. the susceptibility of 15 strains of campylobacter jejuni and of 1 strain of c. coli, isolated from patients with acute gastroenteritis, has been studied against 12 antibiotics with the broth microdilution met ...19873453149
molecular cloning of a species-specific dna probe for campylobacter jejuni.conventional procedures for isolating and identifying campylobacter jejuni are cumbersome and time-consuming. a simpler approach would be to use dna probes to identify these organisms. to obtain such probes we cloned chromosomal dna from c. jejuni into the lambda replacement vector embl 4. recombinant phages were screened for c. jejuni-specific inserts by dna hybridization using chromosomal dna from either c. jejuni or c. coli which had been radioactively labelled with 32p. by this means, recomb ...19873453899
immunoblot and enzyme-linked immunosorbent assays of campylobacter major outer-membrane protein and application to the differentiation of campylobacter species.the major outer-membrane protein (momp) from campylobacter jejuni nctc 11168 was purified by solubilization in triton x-100. whole-cell proteins of campylobacter species and the momp were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, immunoblotting on nitrocellulose paper and enzyme-linked immunoblot assay (elisa) analyses using polyclonal antiserum to the c. jejuni momp. purified momp from c. jejuni nctc 11168 contained a single major protein of 46 kda. whole-cell ...19873453900
the incidence of campylobacter jejuni/coli in infants acute diarrhoeal disease. 19873455653
in vitro study of the antibacterial activity of ofloxacin against recent clinical isolates.the in vitro activity of ofloxacin, a new broad-spectrum antimicrobial agent, was studied by a standardized single disc method. a total of 990 clinical isolates were tested, including 20 strains of anaerobic bacteria. ofloxacin was highly active against 683 strains (70.41%), had intermediate activity against 109 (11.23%) and had no activity against 178 (18.35%). ofloxacin was highly active against e. coli, klebsiella sp., citrobacter sp., proteus mirabilis, proteus morganii, salmonella sp., camp ...19873476290
acid and alkaline campylobacter extracts in the demonstration of antibody response in hyperimmunized rabbits.acid and alkaline extracts from several campylobacter strains were used as antigens in enzyme immunoassay (eia). antibodies were detected in rabbits immunized with formalin-treated, boiled, or autoclaved whole bacteria of different campylobacter subspecies. for the preparation of the extracts, three campylobacter fetus subsp. venerealis strains, three campylobacter fetus subsp. fetus strains, four campylobacter coli, and five campylobacter jejuni strains were used. the acid extracts of campyloba ...19873296648
two-dimensional gel electrophoresis and immunoblotting of campylobacter outer membrane proteins.we characterized outer membrane proteins (omps) from selected campylobacter jejuni, c. coli, and c. fetus strains by two-dimensional gel electrophoresis (2dge), using isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), and by immunoblotting with immune rabbit serum. the flagellar band with a molecular mass of 63 kilodaltons (kda) demonstrated previously by one-dimensional sds-page was shown by 2dge to consist of one or several charge trains, depending u ...19873298060
structural and antigenic properties of lipopolysaccharides from serotype reference strains of campylobacter jejuni.to investigate the molecular basis for heat-stable antigenic diversity in campylobacter jejuni, lipopolysaccharides (lpss) from serotype reference strains and serotyped isolates were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis coupled with silver staining and immunoblotting. by silver staining, only low-mr components, consisting of one major band and as many as three minor bands ranging in mr from 4,500 to 5,000, were detected. however, by immunoblotting with homologous ...19873301678
the aetiology of diarrhoea in children at an urban hospital in bangladesh.enteric pathogens were studied in 104 cases with acute diarrhoea and in 74 age and sex matched concurrent controls. one or more pathogens were isolated from 59.1% of cases compared with 20.4% of controls (p less than 0.001). single enteropathogens were detected in 33.7% and multiple enteropathogens in 25.4% of the cases. enteropathogenic escherichia coli (epec), campylobacter jejuni, rotavirus, vibrio cholerae non-o1 and enterotoxigenic escherichia coli were the major pathogens detected. the hig ...19873303485
clinical aspects of infection with yersinia enterocolitica in adults.yersinia enterocolitica was isolated from the feces of 29 patients over a three-year period following the introduction of a selective culture medium. y. enterocolitica was the third most common enteric pathogen after campylobacter jejuni and salmonella in this series of 3795 specimens from a predominantly adult population. the isolation rate of y. enterocolitica was 0.9% and this represented 15.8% of positive cultures. the usual symptoms of yersinia infection were diarrhea (93%) and abdominal pa ...19873304248
small rodents and other mammals associated with mountain meadows as reservoirs of giardia spp. and campylobacter spp.sixty-five percent (469 of 722) of the fecal samples collected from small rodents in the central washington cascade mountains were positive for giardia spp. trapping studies showed that microtines of the genus microtus were heavily infected with the parasite. morphologically the cysts and trophozoites were of the giardia duodenalis type. small-rodent populations appear to maintain their infection throughout the year. our data suggest that there is no difference in the percentage of positive anim ...19873310881
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...19873312015
in vitro activity of ciprofloxacin compared to trimethoprim-sulfamethoxazole against campylobacter spp., shigella spp. and enterotoxigenic escherichia coli causing travellers' diarrhea in egypt.the in vitro activity of ciprofloxacin against bacterial enteropathogens isolated from cases of travellers' diarrhea in egypt was compared to trimethoprim (tmp) and trimethoprim-sulfamethoxazole (sxt). no resistance to ciprofloxacin was noted for any of the campylobacter jejuni/coli, shigella spp., and enterotoxigenic escherichia coli strains examined. however, resistance to tmp and sxt was noted among these same strains. because of its broad spectrum and lack of resistance, ciprofloxacin is pot ...19873313681
frequency of escherichia coli o157:h7 isolation from stool specimens.during a 6-month period, 7252 faeces specimens were examined for escherichia coli serotype o157:h7. forty-nine specimens (0.7%) from 19 patients yielded this organism. escherichia coli o157:h7 was the third most frequently isolated bacterial pathogen, following campylobacter jejuni and (or) salmonella sp. although regional variation between laboratories determined whether campylobacter jejuni or salmonella was the primary bacterial pathogen isolated, e. coli o157:h7 was consistently isolated mor ...19873319115
human yersinia enterocolitica infection in the eastern cape. a report on 18 cases and a review of yersiniosis in the rsa.yersinia enterocolitica infections with a wide spectrum of clinical presentations have been reported in south africa. an additional 18 cases were encountered in the eastern cape region during the period may 1982 - december 1984. y. enterocolitica isolates comprised 1% of 1,634 faecal examinations performed by the microbiology department of the south african institute for medical research in port elizabeth during this period, compared with isolation rates of 5.1% for shigellae, 3.7% for salmonell ...19873321485
epidemiological studies on salmonella and campylobacter jejuni.an overview is given of investigations concerning the epidemiology of salmonella, carried out at the national institute of public health and environmental hygiene in bilthoven, the netherlands, during the last thirty years. it is made clear that salmonella, because of its ubiquitous occurrence and its large variety in sero- and phage-types, is the organism of choice to study the epidemiological pathways of pathogens between man, animals and the environment. it is demonstrated that these are in f ...19873321686
[reactive arthritis in enteral infections].certain gastrointestinal tract infections can trigger reactive arthritis. the best known triggering agents are yersinia, salmonella, shigella, campylobacter jejuni, and possibly clostridium difficile. the clinical findings show a subacute onset of mono- or oligoarthritis, less often of asymmetric polyarthritis with predominance of the lower limbs. the clinical picture is somewhat modified by the genetic constitution (hla type) of the patient. hla-b27-positive patients appear to have more severe ...19873326361
paper-disc method for campylobacter hippurate-hydrolysis test. a note.paper discs were impregnated with sodium hippurate dissolved in 1/15 phosphate buffer ph 7.2. the discs, each containing 2 mg substrate, were stored in dry state at room temperature. after adding a disc to bacteria suspended in distilled water, and incubated at 37 degrees c for 2 h, one drop of skirrow and benjamin's ninhydrin reagent was added and the reaction was read after re-incubation at 37 degrees c for 10 min. 169 campylobacter strains were tested. the results for a total of 169 strains w ...19873326391
[campylobacter jejuni in patients with diarrhea].in this study the stools 125 patients with diarrhea applied to research hospital of medical school of atatürk university and the stools of 69 healthy persons were investigated for gastroenteritis agents c. jejuni as well as salmonella and shigella. of the zooparasiter agents protozoon and helminths were also investigated. we determined the mentioned agents in 28 of 125 patients (% 22.40). c. jejuni was isolated the most frequent cause of diarrhea with 8.80 percent. c. jejuni was isolated in high ...19873328817
isolating campylobacter jejuni in an atmosphere made oxygen deficient using pyrogallol in a candle jar. 19873330104
campylobacter jejuni infection in the ferret: an animal model of human campylobacteriosis.campylobacter infection in weanling ferrets (mustela putorius furo) was studied as an animal model for enteric campylobacteriosis in persons. the screening of fecal cultures on selective campylobacter media showed that campylobacter jejuni/coli was not present in the normal enteric flora. intragastric feeding of a mixture of cat feed and 2.5 x 10(8) c jejuni isolated from ferrets with naturally occurring proliferative colitis was accomplished. all ferrets (n = 8) became infected on 3 days after ...19873826848
demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus.western blot analysis showed that there is little immunological cross-reactivity of the human pathogens campylobacter jejuni and campylobacter coli with campylobacter pyloridis, except for a very strong cross-reaction between the flagellins. this same antigenic cross-reaction was found with two isolates of gram-negative spiral microaerophilic bacteria that are known to colonize the intestinal mucosa of rodents, but not with the flagellins of a number of other motile bacteria. it is proposed that ...19873818099
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