Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| usefulness of real time pcr for the differentiation and quantification of 652 and jp2 actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva. | the aim of our study is to describe a fast molecular method, able to distinguish and quantize the two different genotypes (652 and jp2) of an important periodontal pathogen: actinobacillus actinomycetemcomitans. the two genotypes show differences in the expression of an important pathogenic factor: the leukotoxin (ltx). in order to evidence this, we performed a real time pcr procedure on the ltx operon, able to recognize aa clinical isolates with different leukotoxic potentials. | 2006 | 16772039 |
| t-cell expression cloning of porphyromonas gingivalis genes coding for t helper-biased immune responses during infection. | exposure of the mouse oral cavity to porphyromonas gingivalis results in the development of gingivitis and periapical bone loss, which apparently are associated with a th1 response to bacterial antigens. we have used this infection model in conjunction with direct t-cell expression cloning to identify bacterial antigens that induce a preferential or biased t helper response during the infectious process. a p. gingivalis-specific cd4 t-cell line derived from mice at 3 weeks postchallenge was used ... | 2006 | 16790769 |
| omega-3 fatty acid effect on alveolar bone loss in rats. | gingival inflammation and alveolar bone resorption are hallmarks of adult periodontitis, elicited in response to oral micro-organisms such as porphyromonas gingivalis. we hypothesized that omega (omega)-3 fatty acids (fa) dietary supplementation would modulate inflammatory reactions leading to periodontal disease in infected rats. rats were fed fish oil (omega-3 fa) or corn oil (n-6 fa) diets for 22 weeks and were infected with p. gingivalis. rats on the omega-3 fa diet exhibited elevated serum ... | 2006 | 16798867 |
| bacterial profile and burden of periodontal infection in subjects with a diagnosis of acute coronary syndrome. | periodontitis has been identified as a potential risk factor in cardiovascular diseases. it is possible that the stimulation of host responses to oral infections may result in vascular damage and the inducement of blood clotting. the aim of this study was to assess the role of periodontal infection and bacterial burden as an explanatory variable to the activation of the inflammatory process leading to acute coronary syndrome (acs). | 2006 | 16805672 |
| surface protease of treponema denticola hydrolyzes c3 and influences function of polymorphonuclear leukocytes. | treponema denticola is a dominant microorganism in human periodontal lesions. one of the major virulence factors of this microorganism is its chymotrypsin-like surface protease, dentilisin. the purpose of this study was to evaluate the effect of dentilisin on human polymorphonuclear leukocytes (pmns). we used chemiluminescence to assess production of o(-)(2) by pmns against t. denticola atcc 35405 and dentilisin-deficient mutant k1. t. denticola atcc 35405 induced production of o(-)(2), whereas ... | 2006 | 16815066 |
| [prevalence of putative periodontal microorganisms in chinese patients with aggressive periodontitis]. | to evaluate the prevalence of putative periodontal microorganism in chinese patients with aggressive periodontitis (agp). | 2006 | 16836903 |
| effectiveness of ultrasonic instruments in the therapy of severe periodontitis: a comparative clinical-microbiological assessment with curettes. | patients with deep periodontal pockets were treated with either vector system (tg) or manual instruments (cg). clinical assessments by supragingival plaque (pl+), gingival index (gi), bleeding on probing (bop), probing depth (pd), clinical attachment level (cal) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. multiplex polymerase chain reaction was used to determine the presence of actinobacillus actinomycetemcomitans, campylobacter rectus, ... | 2006 | 16841550 |
| identification of biofilm proteins in non-typeable haemophilus influenzae. | non-typeable haemophilus influenzae biofilm formation is implicated in a number of chronic infections including otitis media, sinusitis and bronchitis. biofilm structure includes cells and secreted extracellular matrix that is "slimy" and believed to contribute to the antibiotic resistant properties of biofilm bacteria. components of biofilm extracellular matrix are largely unknown. in order to identify such biofilm proteins an ex-vivo biofilm of a non-typeable haemophilus influenzae isolate, or ... | 2006 | 16854240 |
| enhanced biofilm formation and loss of capsule synthesis: deletion of a putative glycosyltransferase in porphyromonas gingivalis. | periodontitis is a biofilm-mediated disease. porphyromonas gingivalis is an obligate anaerobe consistently associated with severe manifestations of this disease. as an opportunistic pathogen, the ability to proliferate within and disseminate from subgingival biofilm (plaque) is central to its virulence. here, we report the isolation of a p. gingivalis transposon insertion mutant altered in biofilm development and the reconstruction and characterization of this mutation in three different wild-ty ... | 2006 | 16855241 |
| antibody response after single-visit full-mouth ultrasonic debridement versus quadrant-wise therapy. | the aim of this study was to compare serum antibody responses to periodontal pathogens after single-visit full-mouth ultrasonic debridement and quadrant-wise therapy. | 2006 | 16856899 |
| treponema denticola in disseminating endodontic infections. | treponema denticola is a consensus periodontal pathogen that has recently been associated with endodontic pathology. in this study, the effect of mono-infection of the dental pulp with t. denticola and with polymicrobial "red-complex" organisms (rc) (porphyromonas gingivalis, tannerella forsythia, and t. denticola) in inducing disseminating infections in wild-type (wt) and severe-combined-immunodeficiency (scid) mice was analyzed. after 21 days, a high incidence (5/10) of orofacial abscesses was ... | 2006 | 16861296 |
| identification of smtb/arsr cis elements and proteins in archaea using the prokaryotic intergenic exploration database (piged). | microbial genome sequencing projects have revealed an apparently wide distribution of smtb/arsr metal-responsive transcriptional regulators among prokaryotes. using a position-dependent weight matrix approach, prokaryotic genome sequences were screened for smtb/arsr dna binding sites using data derived from intergenic sequences upstream of orthologous genes encoding these regulators. sixty smtb/arsr operators linked to metal detoxification genes, including nine among various archaeal species, ar ... | 2006 | 16877320 |
| sampling strategy for intraoral detection of periodontal pathogens before and following periodontal therapy. | the aim of this study was to identify a sampling strategy with high probability for detecting oral colonization by actinobacillus actinomycetemcomitans, porphyromonas gingivalis, eikenella corrodens, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, and treponema denticola before and following mechanical periodontal therapy. | 2006 | 16881801 |
| roles of the host oxidative immune response and bacterial antioxidant rubrerythrin during porphyromonas gingivalis infection. | the efficient clearance of microbes by neutrophils requires the concerted action of reactive oxygen species and microbicidal components within leukocyte secretory granules. rubrerythrin (rbr) is a nonheme iron protein that protects many air-sensitive bacteria against oxidative stress. using oxidative burst-knockout (nadph oxidase-null) mice and an rbr gene knockout bacterial strain, we investigated the interplay between the phagocytic oxidative burst of the host and the oxidative stress response ... | 2006 | 16895445 |
| antimicrobial therapy using a local drug delivery system (arestin) in the treatment of peri-implantitis. i: microbiological outcomes. | to assess the microbiological outcome of local administration of minocycline hydrochloride microspheres 1 mg (arestin) in cases with peri-implantitis and with a follow-up period of 12 months. | 2006 | 16907769 |
| arginine-specific gingipains from porphyromonas gingivalis deprive protective functions of secretory leucocyte protease inhibitor in periodontal tissue. | chronic periodontitis is correlated with porphyromonas gingivalis infection. in this study, we found that the expression of secretory leucocyte protease inhibitor (slpi), an endogenous inhibitor for neutrophil-derived proteases, was reduced in gingival tissues with chronic periodontitis associated with p. gingivalis infection. the addition of vesicles of p. gingivalis decreased the amount of slpi in the media of primary human gingival keratinocytes compared to untreated cultures. we therefore in ... | 2006 | 16907925 |
| comparative genome analysis: selection pressure on the borrelia vls cassettes is essential for infectivity. | at least three species of borrelia burgdorferi sensu lato (bbsl) cause tick-borne lyme disease. previous work including the genome analysis of b. burgdorferi b31 and b. garinii pbi suggested a highly variable plasmid part. the frequent occurrence of duplicated sequence stretches, the observed plasmid redundancy, as well as the mainly unknown function and variability of plasmid encoded genes rendered the relationships between plasmids within and between species largely unresolvable. | 2006 | 16914037 |
| association of eubacterium nodatum and treponema denticola with human periodontitis lesions. | the purpose of the present investigation was to compare the levels, proportions and percentage of sites colonized by 40 bacterial species in subgingival plaque samples from periodontally healthy subjects and patients with chronic periodontitis to seek possible pathogens other than the consensus pathogens porphyromonas gingivalis and tannerella forsythia. | 2006 | 16922925 |
| porphyromonas gingivalis vesicles enhance attachment, and the leucine-rich repeat bspa protein is required for invasion of epithelial cells by "tannerella forsythia". | the human oral cavity harbors more than 500 species of bacteria. periodontitis, a bacterially induced inflammatory disease that leads to tooth loss, is believed to result from infection by a select group of gram-negative periodontopathogens that includes porphyromonas gingivalis, treponema denticola, and "tannerella forsythia" (opinion on name change from tannerella forsythensis pending; formerly bacteroides forsythus). epithelial cell invasion by periodontopathogens is considered to be an impor ... | 2006 | 16926393 |
| novel conserved assembly factor of the bacterial flagellum. | tp0658 (fliw) and its orthologs, conserved proteins of unknown function in treponema pallidum and other species, interact with a c-terminal region of flagellin (flab1-3 in t. pallidum; flic in most other species). mutants of orthologs in bacillus subtilis and campylobacter jejuni (yvif, cj1075) showed strongly reduced motility. tp0658 stabilizes flagellin in a way similar to flis, suggesting that tp0658 is a conserved assembly factor for the bacterial flagellum. | 2006 | 16936039 |
| data mining for proteins characteristic of clades. | a synapomorphy is a phylogenetic character that provides evidence of shared descent. ideally a synapomorphy is ubiquitous within the clade of related organisms and nonexistent outside the clade, implying that it arose after divergence from other extant species and before the last common ancestor of the clade. with the recent proliferation of genetic sequence data, molecular synapomorphies have assumed great importance, yet there is no convenient means to search for them over entire genomes. we h ... | 2006 | 16936320 |
| inhibition of human immunodeficiency virus type 1 entry by a binding domain of porphyromonas gingivalis gingipain. | human immunodeficiency virus (hiv) transmission through saliva is extremely low. several oral components, including secretory immunoglobulin a and secretory leukocyte protease inhibitor, are known as potential inhibitory agents of hiv oral transmission. here we examined anti-hiv activity of oral bacterial components. we showed that recombinant protein hgp44 derived from porphyromonas gingivalis, one of the primary infectious agents of periodontitis, was capable of inhibiting hiv type 1 (hiv-1) r ... | 2006 | 16940103 |
| microbiota of the dorsum of the tongue after plaque accumulation: an experimental study in humans. | the purpose of the present investigation was to determine the effect of the absence of tongue hygiene on the microbiota of the dorsum of the tongue. | 2006 | 16945032 |
| levels of matrix metalloproteinases-8 and -9 with simultaneous presence of periodontal pathogens in gingival crevicular fluid as well as matrix metalloproteinase-9 and cholesterol in blood. | to investigate the levels of matrix metalloproteinase (mmp) -8 and -9 with the simultaneous presence of periodontal pathogens in gingival crevicular fluid (gcf) as well as mmp-9 and cholesterol in blood. although bacterial pathogens are required to initiate the periodontal disease process, in some individuals the reaction to bacteria may lead to an excessive host response, resulting in a general inflammatory response. | 2006 | 16953818 |
| molecular epidemiology of oral treponemes in patients with periodontitis and in periodontitis-resistant subjects. | the etiologic role of oral treponemes in human periodontitis is still under debate. although seen by dark-field microscopy in large numbers, their possible role is still unclear since they comprise some 60 different phylotypes, most of which are still uncultured. to determine their status as mere commensals or opportunistic pathogens, molecular epidemiological studies are required that include both cultured and as-yet-uncultured organisms. here we present such data, comparing treponemal populati ... | 2006 | 16954230 |
| detection of cariogenic streptococcus mutans in extirpated heart valve and atheromatous plaque specimens. | the involvement of oral bacteria in the pathogenesis of cardiovascular diseases has been the focus of attention in many studies, and several periodontal pathogens have been detected in diseased cardiovascular lesions, suggesting relationships between oral microorganisms and cardiovascular diseases. however, no information is available regarding the involvement of cariogenic pathogens such as streptococcus mutans. the presence of oral streptococcal species and periodontitis-related bacteria in 35 ... | 2006 | 16954266 |
| diagnosis of gastric syphilis by direct immunofluorescence staining and real-time pcr testing. | we report on a case of gastric syphilis in a patient with chronic dyspepsia. the diagnosis was established by serology and the demonstration of spirochetes in diffusely inflammed gastric mucosa by staining with a fluorescent monoclonal antibody specific for pathogenic treponemes and by the detection of specific treponemal dna sequences by a real-time pcr. | 2006 | 16954299 |
| refuting phylogenetic relationships. | phylogenetic methods are philosophically grounded, and so can be philosophically biased in ways that limit explanatory power. this constitutes an important methodologic dimension not often taken into account. here we address this dimension in the context of concatenation approaches to phylogeny. | 2006 | 16956399 |
| periodontal therapy in siblings with papillon-lefèvre syndrome and tinea capitis: a report of two cases. | report of clinical and microbiological periodontal findings before and 6 months after treatment of two siblings with papillon-lefèvre syndrome (pls) and tinea capitis. | 2006 | 16970621 |
| biochemical and molecular characterization of the biosynthesis of glutamine and glutamate, two major compatible solutes in the moderately halophilic bacterium halobacillus halophilus. | the moderately halophilic, chloride-dependent bacterium halobacillus halophilus produces glutamate and glutamine as main compatible solutes at external salinities of 1.0 to 1.5 m nacl. the routes for the biosynthesis of these solutes and their regulation were examined. the genome contains two genes potentially encoding glutamate dehydrogenases and two genes for the small subunit of a glutamate synthase, but only one gene for the large subunit. however, the expression of these genes was not salt ... | 2006 | 16980483 |
| molecular analysis of filifactor alocis, tannerella forsythia, and treponema denticola associated with primary endodontic infections and failed endodontic treatment. | the aim of this study was to investigate the presence of strict anaerobes such as filifactor alocis, tannerella forsythia, and treponema denticola in primary and secondary root-infected canals with periapical lesions by molecular analysis and the association of these species with specific endodontic signs and symptoms. microbial samples were taken from 100 root canals, 50 with necrotic pulp tissues (npt, primary infection), and 50 with failed endodontic treatment (fet, secondary infection). dna ... | 2006 | 16982268 |
| the flagellar cytoskeleton of the spirochetes. | the recent discoveries of prokaryotic homologs of all three major eukaryotic cytoskeletal proteins (actin, tubulin, intermediate filaments) have spurred a resurgence of activity in the field of bacterial morphology. in spirochetes, however, it has long been known that the flagellar filaments act as a cytoskeletal protein structure, contributing to their shape and conferring motility on this unique phylum of bacteria. therefore, revisiting the spirochete cytoskeleton may lead to new paradigms for ... | 2006 | 16983197 |
| structural characterization of peptide-mediated inhibition of porphyromonas gingivalis biofilm formation. | porphyromonas gingivalis is a periodontal pathogen whose primary niche is the anaerobic environment of subgingival dental plaque, but initial colonization of the oral cavity is likely to occur on supragingival surfaces that already support robust biofilm communities. our studies have shown that p. gingivalis adheres to streptococcus gordonii through interaction of the minor fimbrial antigen mfa1 with a specific region of the streptococcal sspb polypeptide (residues 1167 to 1193) designated bar. ... | 2006 | 16988253 |
| identification and comparative analysis of sixteen fungal peptidyl-prolyl cis/trans isomerase repertoires. | the peptidyl-prolyl cis/trans isomerase (ppiase) class of proteins is present in all known eukaryotes, prokaryotes, and archaea, and it is comprised of three member families that share the ability to catalyze the cis/trans isomerisation of a prolyl bond. some fungi have been used as model systems to investigate the role of ppiases within the cell, however how representative these repertoires are of other fungi or humans has not been fully investigated. | 2006 | 16995943 |
| identification of mgla-regulated genes reveals novel virulence factors in francisella tularensis. | the facultative intracellular bacterium francisella tularensis causes the zoonotic disease tularemia. f. tularensis resides within host macrophages in vivo, and this ability is essential for pathogenesis. the transcription factor mgla is required for the expression of several francisella genes that are necessary for replication in macrophages and for virulence in mice. we hypothesized that the identification of mgla-regulated genes in the francisella genome by transcriptional profiling of wild-t ... | 2006 | 17000729 |
| the influence of cholesterol, progesterone, 4-androstenedione and testosterone on the growth of treponema denticola atcc 33520 in batch cultures. | previously, we have shown that reference and freshly isolated treponema denticola cultures are capable of metabolising cholesterol, progesterone, 4-androstenedione and testosterone by means of 5alpha-reductase, 3beta-and 17beta-hydroxysteroid dehydrogenase activity [clark dt, soory m. the metabolism of cholesterol and certain hormonal steroids by treponema denticola. steroids. 2006;71:352-63. ]. the aim of the work presented in this paper was to investigate the modulation of t. denticola growth ... | 2006 | 17011804 |
| holo- and apo-cystalysin from treponema denticola: two different conformations. | cystalysin, the key virulence factor in the bacterium treponema denticola responsible for periodontis, is a pyridoxal 5'-phosphate (plp) enzyme which catalyzes, in addition to alpha,beta-elimination of l-cysteine, racemization and transamination of both enantiomers of alanine. in this paper several indicators have been used as probes of the different conformational status of t. denticola cystalysin in the holo and apo form. compared to holoenzyme, the apoenzyme displays an altered reactivity of ... | 2006 | 17014820 |
| denaturing gradient gel electrophoresis as a diagnostic tool in periodontal microbiology. | bacteria play an important role in the initiation and progression of periodontal diseases and are part of a biofilm, which can contain over 100 different species. the aim of the present study was to show the potential of denaturing gradient gel electrophoresis (dgge) as a tool for the detection of clinically relevant species and to compare the results of detection by dgge with those by pcr and culturing. hybridization of the bands from the dgge profiles with species-specific probes was developed ... | 2006 | 17021091 |
| changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16s cloning and sequencing. | the gingival sulcus contains a complex ecosystem that includes many uncultivated bacteria. understanding the dynamics of this ecosystem in transitions between health and disease is important in advancing our understanding of the bacterial etiology of periodontitis. the objective of this longitudinal study was to examine the stability of bacterial colonization in the gingival crevice and to explore the relationship between shifts in microbial composition and changes in periodontal health status u ... | 2006 | 17021095 |
| prospective study of the long term change of the oral flora after radiation therapy. | the aim of this prospective study was to evaluate the long term change in oral pathogens following radiation therapy. | 2006 | 16341728 |
| carpegen real-time polymerase chain reaction vs. anaerobic culture for periodontal pathogen identification. | the aim of this study was to compare two methods of microbiological diagnosis, anaerobic bacterial culture and real-time polymerase chain reaction (pcr), for the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, fusobacterium nucleatum, and treponema denticola. | 2006 | 17064390 |
| dimerization and folding processes of treponema denticola cystalysin: the role of pyridoxal 5'-phosphate. | cystalysin, the key virulence factor in the bacterium treponema denticola responsible for periodontitis, is a homodimeric pyridoxal 5'-phosphate (plp)-c-s lyase. the dimerization process and the urea-induced unfolding equilibrium of holocystalysin were compared with those of the apo form. the presence of plp decreases approximately 4 times the monomer-dimer equilibrium dissociation constant. by using a variety of spectroscopic and analytical procedures, we demonstrated a difference in their unfo ... | 2006 | 17115709 |
| distribution of 10 periodontal bacteria in saliva samples from japanese children and their mothers. | we analyzed the distribution of 10 periodontal bacteria species (porphyromonas gingivalis, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, campylobacter rectus, eikenella corrodens, actinobacillus actinomycetemcomitans, capnocytophaga ochracea, capnocytophaga sputigena, and treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (p. gingivalis, ... | 2006 | 16269128 |
| identification of the microorganisms responsible for periodontopathy by multiplex rt-pcr. | the aim of our research was to identify by bacterial genomic dna analysis the prevalence of five different species of periodontopathogenic bacteria present in the subgingival biofilm, specifically: actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), bacterioides forsytus (bf), treponema denticola (td). for the analysis we used the systematic multiplex-pcr-microdent kit with species-specific primers. we studied a group of 48 subjects, 18 males and ... | 2006 | 17263160 |
| periodontitis as a risk factor of coronary heart diseases? | unstable atherosclerotic plaque is a dangerous clinical state, possibly leading to acute coronary deficiency resulting in cardiac infarction. inflammatory factor's role in creating pathological lesions in the endothelium of coronary vessels is frequently raised. this state may be caused by bacteria able to initiate clot formation in blood vessel and destabilizing atherosclerotic plaque already present. source of these pathogens are chronic inflammatory processes occurring in organism, among them ... | 2006 | 17458056 |
| inflammatory responses of a macrophage/epithelial cell co-culture model to mono and mixed infections with porphyromonas gingivalis, treponema denticola, and tannerella forsythia. | accumulated evidence points to porphyromonas gingivalis, treponema denticola, and tannerella forsythia as three major etiologic agents of chronic periodontitis. epithelial cells and macrophages play a major role in the host response to periodontopathogens, and the secretion of inflammatory mediators and matrix metalloproteinases (mmps) by these host cells is believed to contribute to periodontal tissue destruction. the aim of this study was to investigate the inflammatory response of a macrophag ... | 2006 | 16153871 |
| the glycolytic pathway of trimastix pyriformis is an evolutionary mosaic. | glycolysis and subsequent fermentation is the main energy source for many anaerobic organisms. the glycolytic pathway consists of ten enzymatic steps which appear to be universal amongst eukaryotes. however, it has been shown that the origins of these enzymes in specific eukaryote lineages can differ, and sometimes involve lateral gene transfer events. we have conducted an expressed sequence tag (est) survey of the anaerobic flagellate trimastix pyriformis to investigate the nature of the evolut ... | 2006 | 17123440 |
| comparative genomics and evolution of the hsp90 family of genes across all kingdoms of organisms. | hsp90 proteins are essential molecular chaperones involved in signal transduction, cell cycle control, stress management, and folding, degradation, and transport of proteins. hsp90 proteins have been found in a variety of organisms suggesting that they are ancient and conserved. in this study we investigate the nuclear genomes of 32 species across all kingdoms of organisms, and all sequences available in genbank, and address the diversity, evolution, gene structure, conservation and nomenclature ... | 2006 | 16780600 |
| a database of bacterial lipoproteins (dolop) with functional assignments to predicted lipoproteins. | lipid modification of the n-terminal cys residue (n-acyl-s-diacylglyceryl-cys) has been found to be an essential, ubiquitous, and unique bacterial posttranslational modification. such a modification allows anchoring of even highly hydrophilic proteins to the membrane which carry out a variety of functions important for bacteria, including pathogenesis. hence, being able to identify such proteins is of great value. to this end, we have created a comprehensive database of bacterial lipoproteins, c ... | 2006 | 16585737 |
| paths of lateral gene transfer of lysyl-aminoacyl-trna synthetases with a unique evolutionary transition stage of prokaryotes coding for class i and ii varieties by the same organisms. | while the premise that lateral gene transfer (lgt) is a dominant evolutionary force is still in considerable dispute, the case for widespread lgt in the family of aminoacyl-trna synthetases (aars) is no longer contentious. aarss are ancient enzymes, guarding the fidelity of the genetic code. they are clustered in two structurally unrelated classes. only lysine aminoacyl-trna synthetase (lysrs) is found both as a class 1 and a class 2 enzyme (lysrs1-2). remarkably, in several extant prokaryotes b ... | 2006 | 16529662 |
| algebraic comparison of metabolic networks, phylogenetic inference, and metabolic innovation. | comparison of metabolic networks is typically performed based on the organisms' enzyme contents. this approach disregards functional replacements as well as orthologies that are misannotated. direct comparison of the structure of metabolic networks can circumvent these problems. | 2006 | 16478540 |
| how phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria. | the phosphoenolpyruvate(pep):carbohydrate phosphotransferase system (pts) is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, amino sugars, polyols, and other sugar derivatives. to carry out its catalytic function in sugar transport and phosphorylation, the pts uses pep as an energy source and phosphoryl donor. the phosphoryl group of pep is usually transferred via four distinct proteins (domains) to the transported sugar bo ... | 2006 | 17158705 |
| dynamic evolution of selenocysteine utilization in bacteria: a balance between selenoprotein loss and evolution of selenocysteine from redox active cysteine residues. | selenocysteine (sec) is co-translationally inserted into protein in response to uga codons. it occurs in oxidoreductase active sites and often is catalytically superior to cysteine (cys). however, sec is used very selectively in proteins and organisms. the wide distribution of sec and its restricted use have not been explained. | 2006 | 17054778 |
| oral malodour (halitosis). | 2006 | 16990322 | |
| periodontal disease and systemic conditions: a bidirectional relationship. | for decades, physicians and dentists have paid close attention to their own respective fields, specializing in medicine pertaining to the body and the oral cavity, respectively. however, recent findings have strongly suggested that oral health may be indicative of systemic health. currently, this gap between allopathic medicine and dental medicine is quickly closing, due to significant findings supporting the association between periodontal disease and systemic conditions such as cardiovascular ... | 2006 | 16998613 |
| structural classification of bacterial response regulators: diversity of output domains and domain combinations. | chey-like phosphoacceptor (or receiver [rec]) domain is a common module in a variety of response regulators of the bacterial signal transduction systems. in this work, 4,610 response regulators, encoded in complete genomes of 200 bacterial and archaeal species, were identified and classified by their domain architectures. previously uncharacterized output domains were analyzed and, in some cases, assigned to known domain families. transcriptional regulators of the ompr, narl, and ntrc families w ... | 2006 | 16740923 |
| clinical review: molecular mechanisms underlying the role of antithrombin in sepsis. | in disseminated intravascular coagulation (dic) there is extensive crosstalk between activation of inflammation and coagulation. endogenous anticoagulatory pathways are downregulated by inflammation, thus decreasing the natural anti-inflammatory mechanisms that these pathways possess. supportive strategies aimed at inhibiting activation of coagulation and inflammation may theoretically be justified and have been found to be beneficial in experimental and initial clinical studies. this review ass ... | 2006 | 16542481 |
| on the acquisition of periodontopathic bacteria by children from mothers: a randomized double-masked placebo-controlled trial in bauru, brazil. | objective: the purpose of this study was two-fold: 1) to determine the effects of mechanical and chemical modalities treatments on periodontal clinical and microbiological parameters of mothers; and 2) to determine the subsequent colonization of periodontal pathogens in tongue samples from their infants. design: a total of 168 mothers met inclusion criteria to participate in a randomized double-masked placebo-controlled clinical trial. of those, 121 mothers (and their infants) of 158 seen at bas ... | 2006 | 22267892 |
| periodontal therapy alters gene expression of peripheral blood monocytes. | we investigated the effects of periodontal therapy on gene expression of peripheral blood monocytes. | 2007 | 17716309 |
| hydrogen production by termite gut protists: characterization of iron hydrogenases of parabasalian symbionts of the termite coptotermes formosanus. | cellulolytic flagellated protists in the guts of termites produce molecular hydrogen (h(2)) that is emitted by the termites; however, little is known about the physiology and biochemistry of h(2) production from cellulose in the gut symbiotic protists due to their formidable unculturability. in order to understand the molecular basis for h(2) production, we here identified two genes encoding proteins homologous to iron-only hydrogenases (fe hydrogenases) in pseudotrichonympha grassii, a large ce ... | 2007 | 17766465 |
| high content of proteins containing 21st and 22nd amino acids, selenocysteine and pyrrolysine, in a symbiotic deltaproteobacterium of gutless worm olavius algarvensis. | selenocysteine (sec) and pyrrolysine (pyl) are rare amino acids that are cotranslationally inserted into proteins and known as the 21st and 22nd amino acids in the genetic code. sec and pyl are encoded by uga and uag codons, respectively, which normally serve as stop signals. herein, we report on unusually large selenoproteomes and pyrroproteomes in a symbiont metagenomic dataset of a marine gutless worm, olavius algarvensis. we identified 99 selenoprotein genes that clustered into 30 families, ... | 2007 | 17626042 |
| small but versatile: the extraordinary functional and structural diversity of the beta-grasp fold. | the beta-grasp fold (beta-gf), prototyped by ubiquitin (ub), has been recruited for a strikingly diverse range of biochemical functions. these functions include providing a scaffold for different enzymatic active sites (e.g. nudix phosphohydrolases) and iron-sulfur clusters, rna-soluble-ligand and co-factor-binding, sulfur transfer, adaptor functions in signaling, assembly of macromolecular complexes and post-translational protein modification. to understand the basis for the functional versatil ... | 2007 | 17605815 |
| mining prokaryotic genomes for unknown amino acids: a stop-codon-based approach. | selenocysteine and pyrrolysine are the 21st and 22nd amino acids, which are genetically encoded by stop codons. since a number of microbial genomes have been completely sequenced to date, it is tempting to ask whether the 23rd amino acid is left undiscovered in these genomes. recently, a computational study addressed this question and reported that no trna gene for unknown amino acid was found in genome sequences available. however, performance of the trna prediction program on an unknown trna f ... | 2007 | 17597547 |
| maternal oral health status and preterm low birth weight at muhimbili national hospital, tanzania: a case-control study. | the study examined the relationship between oral health status (periodontal disease and carious pulpal exposure (cpe)) and preterm low-birth-weight (ptlbw) infant deliveries among tanzanian-african mothers at muhimbili national hospital (mnh), tanzania. | 2007 | 17594498 |
| an in vitro biofilm model of subgingival plaque. | numerous biofilm models have been described for the study of bacteria associated with the supragingival plaque. however, there are fewer models available for the study of subgingival plaque. the purpose of this study was to develop and validate a model that closely mimicked the composition of the subgingival flora. | 2007 | 17488440 |
| the crisprdb database and tools to display crisprs and to generate dictionaries of spacers and repeats. | in archeae and bacteria, the repeated elements called crisprs for "clustered regularly interspaced short palindromic repeats" are believed to participate in the defence against viruses. short sequences called spacers are stored in-between repeated elements. in the current model, motifs comprising spacers and repeats may target an invading dna and lead to its degradation through a proposed mechanism similar to rna interference. analysis of intra-species polymorphism shows that new motifs (one spa ... | 2007 | 17521438 |
| oral fluid-based biomarkers of alveolar bone loss in periodontitis. | periodontal disease is a bacteria-induced chronic inflammatory disease affecting the soft and hard supporting structures encompassing the teeth. when left untreated, the ultimate outcome is alveolar bone loss and exfoliation of the involved teeth. traditional periodontal diagnostic methods include assessment of clinical parameters and radiographs. though efficient, these conventional techniques are inherently limited in that only a historical perspective, not current appraisal, of disease status ... | 2007 | 17435132 |
| nursing home-associated pneumonia, hospital-acquired pneumonia and ventilator-associated pneumonia: the contribution of dental biofilms and periodontal inflammation. | 2007 | 17474932 | |
| assessing the evolutionary rate of positional orthologous genes in prokaryotes using synteny data. | comparison of completely sequenced microbial genomes has revealed how fluid these genomes are. detecting synteny blocks requires reliable methods to determining the orthologs among the whole set of homologs detected by exhaustive comparisons between each pair of completely sequenced genomes. this is a complex and difficult problem in the field of comparative genomics but will help to better understand the way prokaryotic genomes are evolving. | 2007 | 18047665 |
| quantitative analysis of mutation and selection pressures on base composition skews in bacterial chromosomes. | most bacterial chromosomes exhibit asymmetry of base composition with respect to leading vs. lagging strands (gc and at skews). these skews reflect mainly those in protein coding sequences, which are driven by asymmetric mutation pressures during replication and transcription (notably asymmetric cytosine deamination) plus subsequent selection for preferred structures, signals, amino acid or codons. the transcription-associated effects but not the replication-associated effects contribute to the ... | 2007 | 17711583 |
| transcriptional regulatory network discovery via multiple method integration: application to e. coli k12. | transcriptional regulatory network (trn) discovery from one method (e.g. microarray analysis, gene ontology, phylogenic similarity) does not seem feasible due to lack of sufficient information, resulting in the construction of spurious or incomplete trns. we develop a methodology, trnd, that integrates a preliminary trn, microarray data, gene ontology and phylogenic similarity to accurately discover trns and apply the method to e. coli k12. the approach can easily be extended to include other me ... | 2007 | 17397539 |
| phylogenetic distribution of translational gtpases in bacteria. | translational gtpases are a family of proteins in which gtpase activity is stimulated by the large ribosomal subunit. conserved sequence features allow members of this family to be identified. | 2007 | 17214893 |
| periopathogenic bacteria in persistent periapical lesions: an in vivo prospective study. | the aim of this in vivo prospective study was to detect the presence of periopathogenic bacteria in persistent periapical lesions and to compare the reliability of two different methods: anaerobic culture and the dna hybridization technique. | 2007 | 17470025 |
| comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction. | the outcome of microbiological diagnostics may depend on the sampling technique. it was the aim of the present study to compare two widely used sampling techniques for subgingival bacteria using quantitative real-time polymerase chain reaction. | 2007 | 17470026 |
| herpesviral-bacterial synergy in the pathogenesis of human periodontitis. | periodontitis is an infectious disease, but the specific mechanisms by which tooth-supportive tissues are lost remain obscure. this article proposes an infectious disease model for periodontitis in which herpesviral-bacterial interactions assume a major etiopathogenic role. | 2007 | 17471038 |
| the microbiological profiles of saliva, supragingival and subgingival plaque and dental caries in adults with and without type 2 diabetes mellitus. | the relationships between suspected bacteria in saliva, yeasts in oral rinse, and supragingival and subgingival plaque versus root surface and coronal caries in adults with type 2 diabetes mellitus and a non-diabetic group were explored. | 2007 | 17488443 |
| bacterial colonization immediately after installation on oral titanium implants. | information on bacterial colonization immediately after dental implant insertion is limited. | 2007 | 17501978 |
| treponema denticola induces interleukin-8 and macrophage chemoattractant protein 1 production in human umbilical vein epithelial cells. | treponema denticola, a major pathogen of periodontitis, has also been detected in the lesions of atherosclerosis. the aim of this study was to investigate induction of chemokine production in human umbilical vein endothelial cells (huvecs) by t. denticola and determine whether those chemokines were degraded by a protease, dentilisin. t. denticola atcc35405 or dentilisin-deficient mutant k1 were added to huvecs and levels of interleukin-8 (il-8) and monocyte chemoattractant protein-1 (mcp-1) in t ... | 2007 | 17533151 |
| microbiological findings after periodontal therapy using curettes, er:yag laser, sonic, and ultrasonic scalers. | to evaluate and compare the microbiological effects of hand instruments, er:yag-laser, sonic, and ultrasonic scalers in patients with chronic periodontitis. patient perception of each treatment was documented. | 2007 | 17555412 |
| an actin-stabilizing peptide conjugate deduced from the major outer sheath protein of the bacterium treponema denticola. | a synthetic peptide conjugated to bovine serum albumin, p34(bsa), based on a 10-mer in the deduced amino acid sequence of the major outer sheath protein of treponema denticola, was found to stabilize actin filaments of fibroblasts. pretreatment of cells with p34(bsa) inhibited the actin disruption induced by cytochalasin d and latrunculin b. p34(bsa) was taken up by the cells and localized among actin filaments. p34(bsa) bound actin from fibroblast lysates, and cell exposure to p34(bsa) led to t ... | 2007 | 17565753 |
| population-based study of salivary carriage of periodontal pathogens in adults. | large, general population-based data on carriage rates of periodontal pathogens hardly exist in the current literature. the objectives of the present study were to examine the salivary detection of aggregatibacter (formerly actinobacillus) actinomycetemcomitans, campylobacter rectus, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis, and treponema denticola in a representative sample of the adult population living in southern finland and to clarify which determinants are a ... | 2007 | 17567788 |
| interleukin- 1 gene polymorphisms as assessed in a 10-year study of patients with early-onset periodontitis. | our 10-year study of early-onset periodontitis (eop) patients includes repeated clinical observations, microbiological characteristics and analysis of genetic polymorphism of il-1a and il-1b genes. twenty patients (age 15-26 years) were divided according to the clinical status in the 4th year into a group with mean number of teeth with bleeding on probing (bp) 9.8 and mean number of teeth with periodontal pocket (pp) 2.23, and a group with mean number of teeth with bp 5.37 and no pp. significant ... | 2007 | 17575917 |
| molecular architecture and divalent cation activation of tvok, a prokaryotic potassium channel. | rck (regulator of conductance of potassium) domains form a family of ligand-binding domains found in many prokaryotic k+ channels and transport proteins. although many rck domains contain an apparent nucleotide binding motif, some are known instead to bind ca2+, which can then facilitate channel opening. here we report on the molecular architecture and ligand activation properties of an rck-containing potassium channel cloned from the prokaryote thermoplasma volcanium. this channel, called tvok, ... | 2007 | 17588939 |
| the chymotrypsin-like protease complex of treponema denticola atcc 35405 mediates fibrinogen adherence and degradation. | treponema denticola is an anaerobic spirochete strongly associated with human periodontal disease. t. denticola bacteria interact with a range of host tissue proteins, including fibronectin, laminin, and fibrinogen. the latter localizes in the extracellular matrix where tissue damage has occurred, and interactions with fibrinogen may play a key role in t. denticola colonization of the damaged sites. t. denticola atcc 35405 showed saturable binding of fluid-phase fibrinogen to the cell surface an ... | 2007 | 17591786 |
| lesion formation and antibody response induced by papillomatous digital dermatitis-associated spirochetes in a murine abscess model. | papillomatous digital dermatitis (pdd), also known as hairy heel wart, is a growing cause of lameness of cows in the u.s. dairy industry. farms with pdd-afflicted cows experience economic loss due to treatment costs, decreased milk production, lower reproductive efficiency, and premature culling. while the exact cause of pdd is unknown, lesion development is associated with the presence of anaerobic spirochetes. this study was undertaken to investigate the virulence and antigenic relatedness of ... | 2007 | 17591787 |
| gamma-glutamyl transpeptidase has a role in the persistent colonization of the avian gut by campylobacter jejuni. | the contribution of gamma-glutamyl transpeptidase (ggt) to campylobacter jejuni virulence and colonization of the avian gut has been investigated. the presence of the ggt gene in c. jejuni strains directly correlated with the expression of ggt activity as measured by cleavage and transfer of the gamma-glutamyl moiety. inactivation of the monocistronic ggt gene in c. jejuni strain 81116 resulted in isogenic mutants with undetectable ggt activity; nevertheless, these mutants grew normally in vitro ... | 2007 | 17600669 |
| detection of azithromycin resistance in treponema pallidum by real-time pcr. | we describe a real-time pcr assay for the discrimination of azithromycin-resistant and -susceptible strains of treponema pallidum. this assay is rapid and allows for as many as 30 clinical specimens to be analyzed simultaneously without the need for dna sequencing. | 2007 | 17620374 |
| identification of 22 candidate structured rnas in bacteria using the cmfinder comparative genomics pipeline. | we applied a computational pipeline based on comparative genomics to bacteria, and identified 22 novel candidate rna motifs. we predicted six to be riboswitches, which are mrna elements that regulate gene expression on binding a specific metabolite. in separate studies, we confirmed that two of these are novel riboswitches. three other riboswitch candidates are upstream of either a putative transporter gene in the order lactobacillales, citric acid cycle genes in burkholderiales or molybdenum co ... | 2007 | 17621584 |
| comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis. | molecular machinery governing bacterial chemotaxis consists of the chea-chey two-component system, an array of specialized chemoreceptors, and several auxiliary proteins. it has been studied extensively in escherichia coli and, to a significantly lesser extent, in several other microbial species. emerging evidence suggests that homologous signal transduction pathways regulate not only chemotaxis, but several other cellular functions in various bacterial species. the availability of genome sequen ... | 2007 | 17628132 |
| use of multiple-displacement amplification and checkerboard dna-dna hybridization to examine the microbiota of endodontic infections. | multiple-displacement amplification (mda) has been used to uniformly amplify bacterial genomes present in small samples, providing abundant targets for molecular analysis. the purpose of this investigation was to combine mda and checkerboard dna-dna hybridization to examine the microbiota of endodontic infections. sixty-six samples were collected from teeth with endodontic infections. nonamplified and amplified samples were analyzed by checkerboard dna-dna hybridization for levels and proportion ... | 2007 | 17634304 |
| relationship of periodontal infection to serum antibody levels to periodontopathic bacteria and inflammatory markers in periodontitis patients with coronary heart disease. | several reports have demonstrated a possible association of periodontal infections with coronary heart disease (chd) by elevated antibody titre to periodontopathic bacteria in chd patients compared with non-diseased controls. although each periodontopathic bacterium may vary in virulence for periodontitis and atherosclerosis, antibody response to multiple bacteria in chd patients has not been understood fully. therefore, serum levels of antibody to 12 periodontopathic bacteria together with othe ... | 2007 | 17645769 |
| minocycline hcl microspheres reduce red-complex bacteria in periodontal disease therapy. | the objective of this trial was to measure the antimicrobial effects of a minocycline hcl microsphere (mm) local drug-delivery system when used as an adjunct to scaling and root planing (srp). dna probe analysis for 40 bacteria was used to evaluate the oral bacteria of 127 subjects with moderate to advanced chronic periodontitis. | 2007 | 17668977 |
| length of guanosine homopolymeric repeats modulates promoter activity of subfamily ii tpr genes of treponema pallidum ssp. pallidum. | in treponema pallidum, homopolymeric guanosine repeats of varying length are present upstream of both subfamily i (tprc, d, f and i) and ii (tpre, g and j) tpr genes, a group of potential virulence factors, immediately upstream of the +1 nucleotide. to investigate the influence of these poly-g sequences on promoter activity, tpre, g, j, f and i promoter regions containing homopolymeric tracts with different numbers of gs, the ribosomal binding site and start codon were cloned in frame with the g ... | 2007 | 17683506 |
| human wild-type alanine:glyoxylate aminotransferase and its naturally occurring g82e variant: functional properties and physiological implications. | human hepatic peroxisomal agt (alanine:glyoxylate aminotransferase) is a plp (pyridoxal 5'-phosphate)-dependent enzyme whose deficiency causes primary hyperoxaluria type i, a rare autosomal recessive disorder. to acquire experimental evidence for the physiological function of agt, the k(eq),(overall) of the reaction, the steady-state kinetic parameters of the forward and reverse reactions, and the pre-steady-state kinetics of the half-reactions of the plp form of agt with l-alanine or glycine an ... | 2007 | 17696873 |
| microbiologic analysis of periodontal pockets and carotid atheromatous plaques in advanced chronic periodontitis patients. | in recent years, many researchers have focused their attention on the ability of periodontal pathogens to colonize atheromatous plaques. nevertheless, a clear correlation between the detection rates of periodontopathic bacterial dna in atheromas and in subgingival plaque samples has not been established. the aim of our study was to assess the presence of five periodontal pathogens (actinobacillus actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, treponema denticola, and tan ... | 2007 | 17760541 |
| the impact of a low-frequency chlorhexidine rinsing schedule on the subgingival microbiota (the teeth clinical trial). | information on the efficacy of chlorhexidine (chx) rinsing on the subgingival microbiota is limited. this study tested if intermittent chx rinsing over 5 years had an impact on the subgingival microbiota. | 2007 | 17760545 |
| a comparison of sensitivities of the conventional and the real-time polymerase chain reaction methods for detection of treponema denticola in periapical abscesses. | 2007 | 17768483 | |
| steroid 5alpha-reductase activity of treponema denticola. | previously we have shown that reference and freshly isolated treponema denticola cultures possess 5alpha-reductase (5alpha-r) and 3beta- and 17beta-hydroxysteroid dehydrogenase activity. a gene matching the 3-oxo-5alpha-steroid 4-dehydrogenase family protein (gene id: 2739284; locus tag: tde2697) has been identified in t. denticola atcc 35405. the aim of the work presented here was to optimize assay conditions and determine steroid substrate specificities for the 5alpha-r activity of t. denticol ... | 2007 | 17803630 |
| correlation between infections with different genotypes of human cytomegalovirus and epstein-barr virus in subgingival samples and periodontal status of patients. | accumulating evidence indicates that herpesviruses may be putative pathogens in various types of periodontal diseases. the present study was performed to examine infections with different genotypes of human cytomegalovirus (hcmv) and epstein-barr virus (ebv) in subgingival samples from a chinese population and to analyze the correlation with periodontal status. a nested pcr assay was used to identify the presence of hcmv, ebv type 1 (ebv-1), and ebv-2; and the amplicons were further analyzed by ... | 2007 | 17804655 |
| association between infection of different strains of porphyromonas gingivalis and actinobacillus actinomycetemcomitans in subgingival plaque and clinical parameters in chronic periodontitis. | the aim of this study was to investigate subgingival infection frequencies of porphyromonas gingivalis and actinobacillus actinomycetemcomitans strains with genetic variation in chinese chronic periodontitis (cp) patients and to evaluate its correlation with clinical parameters. | 2007 | 17266188 |