Publications

identification of the stef gene that encodes a novel guanine nucleotide exchange factor specific for rac1.	the rho family gtpases are involved in a variety of cellular events by changing the organization of actin cytoskeletal networks in response to extracellular signals. however, it is not clearly known how their activities are spatially and temporally regulated. here we report the identification of a novel guanine nucleotide exchange factor for rac1, stef, which is related in overall amino acid sequence and modular structure to mouse tiam1 and drosophila sif proteins. stef protein contains two plec ...	1999	10364228
dominant negative murine serum response factor: alternative splicing within the activation domain inhibits transactivation of serum response factor binding targets.	primary transcripts encoding the mads box superfamily of proteins, such as mef2 in animals and zema in plants, are alternatively spliced, producing several isoformic species. we show here that murine serum response factor (srf) primary rna transcripts are alternatively spliced at the fifth exon, deleting approximately one-third of the c-terminal activation domain. among the different muscle types examined, visceral smooth muscles have a very low ratio of srfdelta5 to srf. increased levels of srf ...	1999	10373507
cloning, mapping and expression of ubl3, a novel ubiquitin-like gene.	a novel drosophila melanogaster gene ubl3 was characterized and shown to be highly conserved in man and caenorhabditis elegans (c. elegans). the human and mouse homologues were cloned and sequenced. ubl3 is a ubiquitin-like protein of unknown function with no conserved homologues in yeast. mapping of the human and mouse ubl3 genes places them within a region of shared gene order between human and mouse chromosomes on human chromosome 13q12-13 and telomeric mouse chromosome 5 (mmu5).	1999	10375635
deletion mapping at 12p12-13 in metastatic prostate cancer.	the identification of homozygous deletions in malignant tissue is a powerful tool for the localization of tumor suppressor genes. representational difference analysis (rda) uses selective hybridization and the polymerase chain reaction (pcr) to isolate regions of chromosomal loss and has facilitated the identification of tumor suppressor genes, such as brca2 and pten. we have recently identified a 1-5-cm homozygous deletion on 12p12-13 in a prostate cancer xenograft and found that 47% of patient ...	1999	10379873
mbp1: a novel mutant p53-specific protein partner with oncogenic properties.	using a yeast two-hybrid screening strategy with a common tumour-derived p53 mutant as bait, we identified several mutant p53-interacting partners including the known proteins wild-type (wt) p53, hubc9 and gbp/pias1. in addition, a novel protein partner was identified which we have termed mbp1, for mutant p53-binding protein 1. mbp1 is a new member of the emerging fibulin gene family, which currently comprises fibulin-1, fibulin-2 and s1-5. expression of mbp1 mrna is differentially regulated bot ...	1999	10380882
characterization of mouse trip6: a putative intracellular signaling protein.	trip6 is a human lim domain-containing protein that has been identified in yeast two-hybrid screens as interacting with a variety of proteins. trip6 has been proposed to transport signals from the cell surface to the nucleus. in this report, we have characterized a mouse cdna encoding trip6. mouse trip6 is highly similar to human trip6, especially in the c-terminal lim domain region, and the in vitro and in vivo mouse trip6 cdna directs the synthesis of a polypeptide with a relative mobility of ...	1999	10395914
tamoxifen resistant breast cancer: coregulators determine the direction of transcription by antagonist-occupied steroid receptors.	pharmacological antagonists of steroid receptor action had been thought to exert their effects by a passive mechanism driven principally by the ability of the antagonist to compete with agonist for the ligand binding site. however, recent analyses of antagonist-occupied receptor function suggest a more complex picture. antagonists can be subdivided into two groups, type i, or pure antagonists, and type ii, or mixed antagonists that can have variable transcriptional activity based upon differenti ...	1999	10418980
characterization of peci, a novel monofunctional delta(3), delta(2)-enoyl-coa isomerase of mammalian peroxisomes.	we report here the identification and characterization of human and mouse peci, a novel gene that encodes a monofunctional peroxisomal delta(3),delta(2)-enoyl-coa isomerase. human and mouse peci were identified on the basis of their sequence similarity to eci1p, a recently characterized peroxisomal delta(3),delta(2)-enoyl-coa isomerase from the yeast saccharomyces cerevisiae. cloning and sequencing of the human peci cdna revealed the presence of a 1077-base pair open reading frame predicted to e ...	1999	10419495
the drosophila ortholog of the human xpg gene.	xeroderma pigmentosum complementation group g (xpg) protein is a junction-specific endonuclease which is indispensable for nucleotide excision repair (ner) of dna in eukaryotes. recent studies have hinted at a second, essential function for the xpg protein in higher eukaryotes. we undertook a comparison of the amino acid sequences of multiple xpg orthologs to determine if a motif or domain could be identified that is conserved uniquely in higher eukaryotes. a search of current databases allowed ...	1999	10395909
a gene responsible for a cuticular hydrocarbon polymorphism in drosophila melanogaster.	drosophila melanogaster is polymorphic for the major cuticular hydrocarbon of females. in most populations this hydrocarbon is 7,11-heptacosadiene, but females from africa and the caribbean usually possess low levels of 7,11-heptacosadiene and high quantities of its position isomer 5,9-heptacosadiene. genetic analysis shows that the difference between these two morphs is due to variation at a single segregating factor located on the right arm of chromosome 3 near map position 51.5 and cytologica ...	1999	10425916
the 5' part of the mouse immunoglobulin kappa locus.	the 5' region of the mouse kappa locus comprises 63 vkappa genes in six contigs of together 1.5 mb, including one which links the region to the central part of the locus. the structures of the contigs were established by detailed restriction mapping of cosmid clones prepared from libraries of mouse c57bl/6 dna and of yeast and bacterial artificial chromosomes (yacs, bacs with mouse dna inserts). pulsed-field gel electrophoresis of yeast artificial chromosome digests indicated that the gaps betwe ...	1999	10427968
identification of a new gene encoding pericentromeric dodeca-satellite binding protein in drosophila melanogaster.	dodeca-satellite (cccgtactcggt)n is a type of tandemly repeated dna sequence located in the pericentromeric region of the third chromosome of drosophila melanogaster and that cross-hybridizes with dna from other species such as arabidopsis, mouse and human. this evolutionary conservation suggests that dodeca-satellite might play an important role in the centromeric function. therefore, the aim of our research was the isolament of genes encoding proteins that might help stabilize these dna struct ...	1999	10428466
a family of structurally related ring finger proteins interacts specifically with the ubiquitin-conjugating enzyme ubcm4.	the ubiquitin-conjugating enzyme ubcm4 was previously shown to be necessary for normal mouse development. as a first step in identifying target proteins or proteins involved in the specificity of ubcm4-mediated ubiquitylation, we have isolated seven cdnas encoding proteins that specifically interact with ubcm4 but with none of the other ubcs tested. this interaction was observed in yeast as well as in mammalian cells. with one exception, all ubcm4-interacting proteins (uips) belong to a family o ...	1999	10431818
allelic loss mapping and physical delineation of a region harboring a putative thymic lymphoma suppressor gene on mouse chromosome 12.	our previous allelic loss analysis of gamma-ray induced thymic lymphomas in f1 hybrid and backcross mice between balb/c and msm strains mapped the tlsr4 region exhibiting a high frequency of allelic loss (62%) to a 2.9 cm interval between the markers d12mit53 and d12mit279 on mouse chromosome 12. to narrow further the interval harboring a putative tumor suppressor gene, a high-density scan has been carried out for informative 361 thymic lymphomas. construction of a physical map of tlsr4 with 3 y ...	1999	10435594
the zinc finger protein a20 interacts with a novel anti-apoptotic protein which is cleaved by specific caspases.	a20 is a cys2/cys2 zinc finger protein which is induced by a variety of inflammatory stimuli and which has been characterized as an inhibitor of cell death by a yet unknown mechanism. in order to clarify its molecular mechanism of action, we used the yeast two-hybrid system to screen for proteins that interact with a20. a cdna fragment was isolated which encoded a portion of a novel protein (txbp151), which was recently found to be a human t-cell leukemia virus type-i (htlv-i) tax-binding protei ...	1999	10435631
the mammalian swi/snf complex and the control of cell growth.	the mammalian swi/snf complex is a chromatin remodelling complex that uses the energy of atp hydrolysis to facilitate access of transcription factors to regulatory dna sequences. this complex, that was initially described as a co-factor for nuclear receptors, has recently been associated with the control of cell growth. two of the subunits known as brg-1 and brm can associate with the retinoblastoma tumour suppressor gene product and co-operate with this protein for repression of e2f activity. i ...	1999	10441072
point mutations throughout the gli3 gene cause greig cephalopolysyndactyly syndrome.	greig cephalopolysyndactyly syndrome, characterized by craniofacial and limb anomalies (gcps; mim 175700), previously has been demonstrated to be associated with translocations as well as point mutations affecting one allele of the zinc finger gene gli3. in addition to gcps, pallister-hall syndrome (phs; mim 146510) and post-axial polydactyly type a (pap-a; mim 174200), two other disorders of human development, are caused by gli3 mutations. in order to gain more insight into the mutational spect ...	1999	10441342
genomic organization and mapping of the human and mouse neuronal beta2-nicotinic acetylcholine receptor genes.	as a first step in determining whether there are polymorphisms in the nicotinic acetylcholine receptor (nachr) genes that are associated with nicotine addiction, we isolated genomic clones of the beta2-nachr genes from human and mouse bac libraries. although cdna sequences were available for the human gene, only the promoter sequence had been reported for the mouse gene. we determined the genomic structures by sequencing 12 kb of the human gene and over 7 kb of the mouse gene. while the sizes of ...	1999	10441742
phosphorylation by p44 map kinase/erk1 stimulates cbp histone acetyl transferase activity in vitro.	the transcriptional coactivator cbp displays an intrinsic histone acetyl transferase (hat) activity which seems to participate in transcriptional activation through the destabilization of nucleosome structure. cbp is involved in the activity of several transcription factors that are nuclear endpoints of intracellular signal transduction pathways. in some instances, the transcription factors are phosphorylated upon cell activation, which induces their interaction with cbp. cbp itself is a phospho ...	1999	10448085
human chromosome 3 and pig chromosome 13 show complete synteny conservation but extensive gene-order differences.	a comparative map of human chromosome 3 (hsa 3) and pig chromosome 13 (ssc 13) was constructed using physically assigned pig sequence-tagged sites (stss). pig stss representing 11 hsa 3 genes, including v-raf-1 murine leukemia viral oncogene homolog 1 (raf1), retinoic acid beta receptor (rarb), cholecystokinin (cck), pituitary transcription factor 1 (pou1f1), ceruloplasmin (cp), guanine nucleotide binding protein, alpha-inhibiting polypeptide 2 (gnai2), sucrase-isomaltase (si), rhodopsin (rho), ...	1999	10449917
protective effect of the yeast glucomannan against cyclophosphamide-induced mutagenicity.	glucomannan (gm) isolated from candida utilis with molecular weight 30 kda was administered either intraperitoneally or orally prior to cyclophosphamide (cp) injection and its effect on the frequency of micronuclei was evaluated in polychromatic erythrocytes of mouse bone marrow. gm administration by either route decreased significantly (p<0.002) the clastogenic effect of cp. the protective effect was concentration-dependent, with a higher decrease achieved by 200 mg/kg than by 100 mg/kg b. wt. ...	1999	10477345
coactivators for the orphan nuclear receptor roralpha.	a mutation in the nuclear orphan receptor roralpha results in a severe impairment of cerebellar development by unknown mechanisms. we have shown previously that roralpha contains a strong constitutive activation domain in its c terminus. we therefore searched for mammalian roralpha coactivators using the minimal activation domain as bait in a two-hybrid screen. several known and putative coactivators were isolated, including glucocorticoid receptor-interacting protein-1 (grip-1) and peroxisome p ...	1999	10478845
random chromosome segregation without meiotic arrest in both male and female meiocytes of a dmc1 mutant of arabidopsis.	in yeast, the dmc1 gene is required for interhomolog recombination, which is an essential step for bivalent formation and the correct partition of chromosomes during meiosis i. by using a reverse genetics approach, we were able to identify a t-dna insertion in atdmc1, the arabidopsis homolog of dmc1. homozygotes for the atdmc1 insertion failed to express atdmc1, and their residual fertility was 1.5% that of the wild type. complete fertility was restored in mutant plants when a wild-type copy of ...	1999	10488231
ubiquitous expression and embryonic requirement for rna polymerase ii coactivator subunit srb7 in mice.	mammalian rna polymerase ii complexes and coactivators containing homologs of yeast srb/med proteins have been isolated recently from tissue culture cells. the yeast srb/med complex is involved in global gene expression and is essential, but it is not yet known if its mammalian counterparts are broadly expressed in tissues or if they are essential. we have isolated the murine gene encoding srb7, an srb/med complex protein whose sequence and function is highly conserved between yeast and humans. ...	1999	10500093
cooperation of six and eya in activation of their target genes through nuclear translocation of eya.	drosophila sine oculis and eyes absent genes synergize in compound-eye formation. the murine homologues of these genes, six and eya, respectively, show overlapping expression patterns during development. we hypothesized that six and eya proteins cooperate to regulate their target genes. cotransfection assays were performed with various combinations of six and eya to assess their effects on a potential natural target, myogenin promoter, and on a synthetic promoter, the thymidine kinase gene promo ...	1999	10490620
emk protein kinase-null mice: dwarfism and hypofertility associated with alterations in the somatotrope and prolactin pathways.	gene trapping was used in embryonic stem (es) cells in an attempt to inactivate genes involved in development. the emk (elkl motif kinase) gene has been disrupted and a mutant mouse line derived. previous work had shown that emk kinases, called mark in the rat, exert a major control on microtubule stability by phosphorylating microtubule-associated proteins and that genes homologous to emk in yeast or caenorhabditis elegans are essential for cell and embryonic polarity. although we found the emk ...	1999	10491259
functional screening of an asthma qtl in yac transgenic mice.	many quantitative trait loci (qtls) contributing to genetically complex conditions have been discovered, but few causative genes have been identified. this is mainly due to the large size of qtls and the subtle connection between genotype and quantitative phenotype associated with these conditions. transgenic mice have been successfully used to analyse well-characterized genes suspected of contributing to quantitative traits. although this approach is powerful for examining one gene at a time, i ...	1999	10508526
cloning and characterization of human and mouse prosc (proline synthetase co-transcribed) genes.	large-scale dna sequencing, coupled with in silico gene trapping, is a robust approach to identifying unknown genes in selected genomic regions. using this approach we have isolated a novel human gene, prosc (for proline synthetase co-transcribed [bacterial homolog]), from human chromosome 8p11.2, and its mouse counterpart. the human prosc gene spanned 17 kb of genomic dna; its cdna was 2530 bp long, with 8 exons that included an open reading frame of 825 bp (275 amino acids). the mouse cdna (pr ...	1999	10496079
chicken ovalbumin upstream promoter-transcription factor ii, a new partner of the glucose response element of the l-type pyruvate kinase gene, acts as an inhibitor of the glucose response.	transcription of the l-type pyruvate kinase (l-pk) gene is induced by glucose in the presence of insulin and repressed by glucagon via cyclic amp. the dna regulatory sequence responsible for mediating glucose and cyclic amp responses, called glucose response element (glre), consists of two degenerated e boxes spaced by 5 base pairs and is able to bind basic helix-loop-helix/leucine zipper proteins, in particular the upstream stimulatory factors (usfs). from ex vivo and in vivo experiments, it ap ...	1999	10497199
sequence analysis of sla2 of the dimorphic yeasts candida albicans and yarrowia lipolytica.	we report the complete nucleotide sequence of sla2 of the dimorphic yeasts candida albicans and yarrowia lipolytica. in saccharomyces cerevisiae, sla2 codes for an actin binding protein. the deduced amino acid (aa) sequences of c. albicans casla2p and y. lipolytica ylsla2p consist of 1063 and 1054 aa, respectively. the alignment of the deduced proteins of saccharomyces cerevisiae, y. lipolytica and c. albicans shows regions of identity in the n-terminal part of the proteins, which are essential ...	1999	10514569
requirement of an e1a-sensitive coactivator for long-range transactivation by the beta-globin locus control region.	four erythroid-specific dnase i-hypersensitive sites at the 5'-end of the beta-globin locus confer high-level transcription to the beta-globin genes. to identify coactivators that mediate long-range transactivation by this locus control region (lcr), we assessed the influence of e1a, an inhibitor of the cbp/p300 histone acetylase, on lcr function. e1a strongly inhibited transactivation of agamma- and beta-globin promoters by the hs2, hs2-hs3, and hs1-hs4 subregions of the lcr in human k562 and m ...	1999	10480893
the mammalian hsf4 gene generates both an activator and a repressor of heat shock genes by alternative splicing.	the expression of heat shock genes is controlled at the level of transcription by members of the heat shock transcription factor family in vertebrates. hsf4 is a mammalian factor characterized by its lack of a suppression domain that modulates formation of dna-binding homotrimer. here, we have determined the exon structure of the human hsf4 gene and identified a major new isoform, hsf4b, derived by alternative rna splicing events, in addition to a previously reported hsf4a isoform. in mouse tiss ...	1999	10488131
export pumps for anionic conjugates encoded by mrp genes.	several members of the multidrug resistance protein (mrp) family mediate the atp-dependent transport of amphiphilic anions across membranes. the substrate specificity of recombinant human mrp1 has been most extensively defined by use of inside-out membrane vesicles. substrates include the glutathione s-conjugate leukotriene c4, 17 beta-glucuronosyl estradiol, glucuronosyl bilirubin, glutathione disulfide, in addition to the fluorescent lipophilic anion fluo-3. these substances are also substrate ...	1999	10470375
expression profile of the copper homeostasis gene, ratox1, in the rat brain.	in humans the regulation of cellular copper homeostasis is essential for proper organ development and function. a novel cytosolic protein, named atox 1, was recently identified in yeast that functions in shuttling intracellular mononuclear copper [cu(i)] to copper-requiring proteins. atox 1 and its human homolog, hatox1, are members of an emerging family of proteins termed copper chaperones that are involved in the maintenance of copper homeostasis. northern blot analysis demonstrates that atox ...	1999	10473283
characterization of a mammalian gene related to the yeast ccr4 general transcription factor and revealed by transposon insertion.	murine intracisternal a-particles (iaps) are reiterated retrovirus-like transposable elements that can act as insertional mutagens. accordingly, we previously identified a chimeric transcript initiated at an iap promoter and extending through a 3'-located open reading frame with significant similarity to the c-terminal domain of the yeast ccr4 general transcription factor. in this report, we characterize the corresponding murine gene, mccr4, and its human homologue, thus providing the first desc ...	1999	10521507
hybridization-based karyotyping of mouse chromosomes: hybridization-bands.	we have developed a method, which we have named hybridization-banding, to identify simultaneously all chromosomes in a mouse metaphase spread. the method uses a combination of hybridization probes labeled with a single fluor to yield a simple, unique, readily identifiable hybridization pattern on each chromosome. the method is superior to giemsa- or fluorescence-based banding methods for chromosome identification because the hybridization patterns are simpler and easier to identify, and unique p ...	1999	10516429
a multicopper oxidase gene from candida albicans: cloning, characterization and disruption.	a multicopper oxidase gene from the human pathogenic yeast candida albicans was isolated and characterized. an open reading frame of 1872 bp, designated cafet3, was identified, encoding a predicted protein of 624 amino acids and a molecular mass of 70.5 kda. the identity between the deduced amino acid sequences of cafet3 and the saccharomyces cerevisiae fet3 gene is 55%. cafet3 was localized on chromosome 6. a null mutant (fet3delta/fet3delta) was constructed by sequential gene disruption. unlik ...	1999	10517594
heme oxygenase: recent advances in understanding its regulation and role.	heme oxygenase (ho) is responsible for the physiological breakdown of heme into equimolar amounts of biliverdin, carbon monoxide, and iron. three isoforms (ho-1, ho-2, and ho-3) have been identified. ho-1 is ubiquitous and its mrna and activity can be increased several-fold by heme, other metalloporphyrins, transition metals, and stimuli that induce cellular stress. ho-1 is recognized as a major heat shock/stress response protein. recent work from our laboratory has demonstrated several potentia ...	1999	10519165
mutations in npc1 highlight a conserved npc1-specific cysteine-rich domain.	niemann-pick type ii disease is an autosomal recessive disorder characterized by a defect in intracellular trafficking of sterols. we have determined the intron/exon boundaries of eight exons from the conserved 3' portion of npc1, the gene associated with most cases of the disease. sscp analyses were designed for these exons and were used to identify the majority of mutations in 13 apparently unrelated families. thirteen mutations were found, accounting for 19 of the 26 alleles. these mutations ...	1999	10521290
a novel regulator of g protein signalling in yeast, rgs2, downregulates glucose-activation of the camp pathway through direct inhibition of gpa2.	we have characterized a novel member of the recently identified family of regulators of heterotrimeric g protein signalling (rgs) in the yeast saccharomyces cerevisiae. the yor107w/rgs2 gene was isolated as a multi-copy suppressor of glucose-induced loss of heat resistance in stationary phase cells. the n-terminal half of the rgs2 protein consists of a typical rgs domain. deletion and overexpression of rgs2, respectively, enhances and reduces glucose-induced accumulation of camp. overexpression ...	1999	10523302
mammalian cdc7-dbf4 protein kinase complex is essential for initiation of dna replication.	the cdc7-dbf4 kinase is essential for regulating initiation of dna replication in saccharomyces cerevisiae. previously, we identified a human cdc7 homolog, hscdc7. in this study, we report the identification of a human dbf4 homolog, hsdbf4. we show that hsdbf4 binds to hscdc7 and activates hscdc7 kinase activity when hsdbf4 and hscdc7 are coexpressed in insect and mammalian cells. hsdbf4 protein levels are regulated during the cell cycle with a pattern that matches that of hscdc7 protein kinase ...	1999	10523313
requirement for ras/rac1-mediated p38 and c-jun n-terminal kinase signaling in stat3 transcriptional activity induced by the src oncoprotein.	signal transducers and activators of transcription (stats) are transcription factors that mediate normal biologic responses to cytokines and growth factors. however, abnormal activation of certain stat family members, including stat3, is increasingly associated with oncogenesis. in fibroblasts expressing the src oncoprotein, activation of stat3 induces specific gene expression and is required for cell transformation. although the src tyrosine kinase induces constitutive stat3 phosphorylation on ...	1999	10523640
in silico detection of control signals: mrna 3'-end-processing sequences in diverse species.	we have investigated mrna 3'-end-processing signals in each of six eukaryotic species (yeast, rice, arabidopsis, fruitfly, mouse, and human) through the analysis of more than 20,000 3'-expressed sequence tags. the use and conservation of the canonical aauaaa element vary widely among the six species and are especially weak in plants and yeast. even in the animal species, the aauaaa signal does not appear to be as universal as indicated by previous studies. the abundance of single-base variants o ...	1999	10570197
interaction of the metalloprotease disintegrins mdc9 and mdc15 with two sh3 domain-containing proteins, endophilin i and sh3px1.	metalloprotease disintegrins (a disintegrin and metalloprotease (adam) and metalloprotease, disintegrin, cysteine-rich proteins (mdc)) are a family of membrane-anchored glycoproteins that function in diverse biological processes, including fertilization, neurogenesis, myogenesis, and ectodomain processing of cytokines and other proteins. the cytoplasmic domains of adams often include putative signaling motifs, such as proline-rich sh3 ligand domains, suggesting that interactions with cytoplasmic ...	1999	10531379
identification and characterization of the mouse muts homolog 5: msh5.	we have identified and characterized the complete cdna and gene for the mouse muts homolog 5 (msh5), as a step toward understanding the molecular genetic mechanisms involved in the biological function of this new muts homologous protein in mammals. the msh5 cdna contains a 2502-bp open reading frame (orf) that encodes an 833-amino acid protein with a predicted molecular weight of 92.6 kda, which shares 89.8% amino acid sequence identity with the human hmsh5 protein. northern blot analysis demons ...	1999	10556423
human very long-chain acyl-coa synthetase and two human homologs: initial characterization and relationship to fatty acid transport protein.	several human genes with a high degree of homology to rat very long-chain acyl-coa synthetase (rvlcs) and mouse fatty acid transport protein (mfatp) were identified. full-length cdna clones were obtained for three genes, and predicted amino acid sequences were generated. initial characterization indicated that one gene was most likely hvlcs, the human ortholog of rvlcs. the other two (hvlcs-h1 and hvlcs-h2) were more closely related to rvlcs than to mfatp. phylogenetic analysis of amino acid seq ...	1999	10471116
expression and secretion of a biologically active mouse sonic hedgehog protein by the methylotrophic yeast pichia pastoris.	we have successfully secreted the amino-terminal functional domain of mouse sonic hedgehog protein (shh) into culture fluid using a yeast pichia pastoris expression system. a cdna fragment encoding the amino-terminal domain of mouse shh was inserted downstream of the saccharomyces cerevisiae alpha-mating factor secretion signal. the dna fragment was introduced into the host genome by the spheroplast transformation method. transformants were selected based on their resistance to g418: his+ transf ...	1999	10531654
notch signaling imposes two distinct blocks in the differentiation of c2c12 myoblasts.	notch signal transduction regulates expression of downstream genes through the activation of the dna-binding protein su(h)/cbf1. in drosophila most of notch signaling requires su(h); however, some notch-dependent processes occur in the absence of su(h) suggesting that notch signaling does not always involve activation of this factor. using constitutively active forms of notch lacking cbf1-interacting sequences we identified a notch signaling pathway that inhibits myogenic differentiation of c2c1 ...	1999	10079231
a transforming mutation enhances the activity of the c-kit soluble tyrosine kinase domain.	an activating mutation (dy814) located in the catalytic domain of the c-kit receptor has been found in mastocytomas from human, mouse and rat. we evaluated the enzymic properties of purified wild-type (wt) and dy814 tyrosine kinase domains expressed in pichia pastoris. a linker encoding the flag epitope was fused to c-kit cdna species, enabling affinity purification of the proteins with anti-flag antibodies. yeast lysates expressing dy814 contained multiple tyrosine-phosphorylated proteins, wher ...	1999	9931308
t-star/etoile: a novel relative of sam68 that interacts with an rna-binding protein implicated in spermatogenesis.	rbm is an rna-binding protein encoded on the y chromosome in mammals and is expressed only in the nuclei of male germ cells. genetic evidence from infertile men implicates it in spermatogenesis, but its function is unknown. of a number of potential partners for rbm identified by a yeast two-hybrid screen with testis cdna, the most frequent isolates encoded a novel rna-binding protein, termed t-star, that is closely related to sam68, an src-associated protein of unknown function. the mouse homolo ...	1999	10332027
a mammalian lysosomal membrane protein confers multidrug resistance upon expression in saccharomyces cerevisiae.	mouse transporter protein (mtp) is a highly conserved polytopic membrane protein present in mammalian lysosomes and endosomes. the role of mtp in regulating the in vivo subcellular distribution of numerous structurally distinct small molecules has been examined in this study by its expression in a drug-sensitive strain of the yeast saccharomyces cerevisiae. surprisingly, the expression of mtp in membranes of an intracellular compartment resulted in a cellular resistance or hypersensitivity to a ...	1999	10212276
isolation of ku70-binding proteins (kubs).	dna-dependent protein kinase (dna-pk) plays a critical role in resealing dna double-stand breaks by non-homologous end joining. aside from dna-pk, xrcc4 and dna ligase iv, other proteins which play a role(s) in this repair pathway remain unknown; dna-pk contains a catalytic subunit (dna-pkcs) and a dna binding subunit (ku70 and ku80). we isolated ku70-binding proteins (kub1-kub4) using yeast two-hybrid analyses. sequence analyses revealed kub1 to be apolipoprotein j (apoj), also known as x-ray-i ...	1999	10219089
heterodimeric interactions between chicken ovalbumin upstream promoter-transcription factor family members arp1 and ear2.	members of the chicken ovalbumin upstream promoter-transcription factor (coup-tf) subfamily of orphan nuclear receptors, which minimally includes coup-tfi and arp1, are highly expressed in brain and are generally considered to be constitutive repressors of transcription. we have used a yeast two-hybrid system to isolate proteins expressed in brain that interact with arp1. one of the proteins isolated in this screen was ear2, another orphan receptor that has been suggested to be a member of the c ...	1999	10318855
the mouse major histocompatibility complex: some assembly required.	we have assembled a contig of 81 yeast artificial chromosome clones that spans 8 mb and contains the entire major histocompatibility complex (mhc) from mouse strain c57bl/6 (h2b), and we are in the process of assembling an mhc contig of bacterial artificial chromosome (bac) clones from strain 129 (h2bc), which differs from c57bl/6 in the h2-q and h2-t regions. the current bac contig extends from tapasin to d17leh89 with gaps in the class ii, h2-q, and distal h2-m regions. only four bac clones we ...	1999	10319263
identification of chip, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions.	the chaperone function of the mammalian 70-kda heat shock proteins hsc70 and hsp70 is modulated by physical interactions with four previously identified chaperone cofactors: hsp40, bag-1, the hsc70-interacting protein hip, and the hsc70-hsp90-organizing protein hop. hip and hop interact with hsc70 via a tetratricopeptide repeat domain. in a search for additional tetratricopeptide repeat-containing proteins, we have identified a novel 35-kda cytoplasmic protein, carboxyl terminus of hsc70-interac ...	1999	10330192
a human immunoglobulin lambda locus is similarly well expressed in mice and humans.	transgenic mice carrying a 380-kb region of the human immunoglobulin (ig) lambda light (l) chain locus in germline configuration were created. the introduced translocus on a yeast artificial chromosome (yac) accommodates the most proximal iglambda variable region (v) gene cluster, including 15 vlambda genes that contribute to >60% of lambda l chains in humans, all jlambda-clambda segments, and the 3' enhancer. huiglambdayac mice were bred with animals in which mouse igkappa production was silenc ...	1999	10330440
acn9 is a novel protein of gluconeogenesis that is located in the mitochondrial intermembrane space.	previous studies have indicated that the acn9 protein is involved in gluconeogenesis. yeast mutants defective in the acn9 gene display phenotypes identical with mutants defective in metabolic enzymes required for carbon assimilation. these phenotypes include the inability to utilize acetate as a carbon and energy source, elevated levels of enzymes of the glyoxylate cycle, gluconeogenesis and acetyl-coa mobilization, and a deficiency in de novo synthesis of glucose from ethanol. the acn9 gene was ...	1999	10103055
the mechanisms of death of an erythroleukemic cell line by p53: involvement of the microtubule and mitochondria.	a murine erythroleukemic cell line (1-2-3) which expresses only the temperature-sensitive mutant p53 gene (ala-to-val substitution at codon 135) was established. these cells showed typical characteristics of apoptosis, when they were cultured at 32 degrees c. in this process, p53 recovered the wild-type p53 function and the expression of the p21 (waf1/cip1/sdi1), cyclin g1 and gadd45 genes was increased. however, no significant changes were detected in the expression of the mdm2, bcl-2, bax, fas ...	1999	10194136
cloning, expression, and chromosomal mapping of a human atpase ii gene, member of the third subfamily of p-type atpases and orthologous to the presumed bovine and murine aminophospholipid translocase.	recently, a p-type atpase was cloned from bovine chromaffin granules (b-atpase ii) and a mouse teratocarcinoma cell line (m-atpase ii) and was shown to be homologous to the saccharomyces cerevisiae drs2 gene, the inactivation of which resulted in defective transport of phosphatidylserine. here, we report the cloning from a human skeletal muscle cdna library of a human atpase ii (h-atpase ii), orthologous to the presumed bovine and mouse aminophospholipid translocase (95.3 and 95.9% amino acid id ...	1999	10198212
preparation of yac end fragments from the whitehead/mit mouse yac library prml vectors.		1999	10090972
delta 9-fatty acid desaturase from arachidonic acid-producing fungus. unique gene sequence and its heterologous expression in a fungus, aspergillus.	based on the sequence information for delta 9-desaturase genes (from rat, mouse and yeast), which are involved in the desaturation of palmitic acid and stearic acid to palmitoleic acid and oleic acid, respectively, the corresponding cdna and genomic gene were cloned from the fungal strain, mortierella alpina 1s-4, which industrially produces arachidonic acid. there was a cytochrome b5-like domain linked to the carboxyl terminus of this mortierella desaturase, as also seen in the yeast delta 9-de ...	1999	10091601
tlk, a novel evolutionarily conserved murine serine threonine kinase, encodes multiple testis transcripts.	hypothesizing that genes important in meiotic processes in mammals might have evolutionarily conserved counterparts in lower organisms, we used the yeast ime2 meiotic gene (serine threonine kinase) as a probe for screening a mouse testis cdna library. this screening resulted in identification of a novel putative serine threonine kinase. although it did not exhibit significant homology to ime2, it did show significant sequence homology to the tousled kinase in arabidopsis. tousled is associated w ...	1999	10092119
hsp27 multimerization mediated by phosphorylation-sensitive intermolecular interactions at the amino terminus.	distinct biochemical activities have been reported for small and large molecular complexes of heat shock protein 27 (hsp27), respectively. using glycerol gradient ultracentrifugation and chemical cross-linking, we show here that chinese hamster hsp27 is expressed in cells as homotypic multimers ranging from dimers up to 700-kda oligomers. treatments with arsenite, which induces phosphorylation on ser15 and ser90, provoked a major change in the size distribution of the complexes that shifted from ...	1999	10092617
rna surveillance. unforeseen consequences for gene expression, inherited genetic disorders and cancer.	messenger rnas are monitored for errors that arise during gene expression by a mechanism called rna surveillance, with the result that most mrnas that cannot be translated along their full length are rapidly degraded. this ensures that truncated proteins are seldom made, reducing the accumulation of rogue proteins that might be deleterious. the pathway leading to accelerated mrna decay is referred to as nonsense-mediated mrna decay (nmd). the proteins that catalyze steps in nmd in yeast serve tw ...	1999	10098411
hpop4: a new protein subunit of the human rnase mrp and rnase p ribonucleoprotein complexes.	rnase mrp is a ribonucleoprotein particle involved in the processing of pre-rrna. the rnase mrp particle is structurally highly related to the rnase p particle, which is involved in pre-trna processing. their rna components fold into a similar secondary structure and they share several protein subunits. we have identified and characterised human and mouse cdnas that encode proteins homologous to ypop4p, a protein subunit of both the yeast rnase mrp and rnase p complexes. the human pop4 cdna enco ...	1999	10352175
identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery.	we have identified a approximately 140 amino acid domain that is shared by a variety of proteins in budding and fission yeast, nematode, rat, mouse, frog, oat, and man. typically, this domain is located within 20 residues of the n-terminus of the various proteins. the percent identity among the domains in the 12 proteins ranges from 42 to 93%, with 16 absolutely conserved residues: n-x(11-13)-v-x2-a-t-x(34-36)-r-x(7-8)-w-r-x3-k-x12-g-x-e-x15 -l-x11-12-d-x-g-r-x11-d-x7-r. even though these protei ...	1999	10048338
cloning of a gadd34-like gene that interacts with the zinc-finger transcription factor which binds to the p21(waf) promoter.	a histone deacetylase inhibitor has been shown to induce differentiation of many cancer cells and senescence-like state of human fibroblasts. previously, our data suggested that the region responsive to trichostatin a (tsa), a specific inhibitor of histone deacetylase, treatment in the p21(waf1) promoter is located -100 bp upstream from transcription initiation site and contains a gc-box where both sp1 and sp3 are responsible. here we show that another zinc-finger transcription factor, bfcol1, w ...	1999	10066455
human and mouse homologs of the schizosaccharomyces pombe rad17+ cell cycle checkpoint control gene.	the schizosaccharomyces pombe rad17+ cell cycle checkpoint control gene is required for s-phase and g2/m arrest in response to both dna damage and incomplete dna replication. we isolated and characterized the putative human (rad17sp) and mouse (mrad17sp) homologs of the s. pombe rad17 (rad17sp) protein. the human rad17sp open reading frame (orf) encodes a protein of 681 amino acids; the mrad17sp orf codes for a protein of 688 amino acids. the mrad17sp messenger is highly expressed in the testis ...	1999	9933569
molecular cloning and sequencing of cdnas encoding homologues of human ku70 and ku80 autoantigen from xenopus and their expression in various xenopus tissues.	we isolated cdna clones encoding ku70 and ku80 homologues of xenopus laevis from a cdna library prepared from xenopus oocytes. the nucleotide sequences of these ku70 and ku80 homologues have coding sequences of 1833 bp and a 611 aa protein, and 2178 bp and a 726 aa protein, respectively. the amino acid sequences deduced from the open reading frame of the ku70 and ku80 cdna clones were highly homologous to those from ku genes previously isolated, such as human (ca. 65% and ca. 62% identity, respe ...	1999	10209269
signal presequences increase mitochondrial permeability and open the multiple conductance channel.	we have reported that the signal presequence of cytochrome oxidase subunit iv from neurospora crassa increases the permeability of isolated rat liver mitochondria [p. m. sokolove and k. w. kinnally (1996) arch. biochem. biophys. 336, 69] and regulates the behavior of the mutiple conductance channel (mcc) of yeast inner mitochondrial membrane [t. a. lohret and k. w. kinnally (1995) j. biol. chem. 270, 15950]. here we examine in greater detail the action of a number of mitochondrial presequences f ...	1999	10334870
subunits of the eukaryotic cytosolic chaperonin cct do not always behave as components of a uniform hetero-oligomeric particle.	the chaperonin cct is an hetero-oligomeric molecular chaperone complex. studies in yeast suggest each of its eight gene products are required for its major identified functions in producing native tubulins and actins. however, it is unclear whether these eight components always form a single particle, covering all functions, or else can also exist as heterogeneous mixtures and/or free subunits in cells. using mouse p19 embryonal carcinoma cells, which divide rapidly, yet in retinoic acid adopt a ...	1999	10082421
mitogen-activated protein kinases: specific messages from ubiquitous messengers.		1999	10082509
dna cleavage and degradation by the sbccd protein complex from escherichia coli.	the sbccd protein is a member of a group of nucleases found in bacteriophage t4 and t5, eubacteria, archaebacteria, yeast, drosophila, mouse and man. evidence from electron microscopy has revealed a distinctive structure consisting of two globular domains linked by a long region of coiled coil, similar to that predicted for the members of the smc family. that a nuclease should have such an unusual structure suggests that its mode of action may be complex. here we show that the protein degrades d ...	1999	9927737
analysis of a conditional degradation signal in yeast and mammalian cells.	the n-end rule pathway is a ubiquitin-dependent proteolytic system, the targets of which include proteins that bear destabilizing n-terminal residues. the latter are a part of the degradation signal called the n-degron. arg-dhfrts, an engineered n-end rule substrate, bears n-terminal arginine (a destabilizing residue) and dhfrts [a temperature-sensitive mouse dihydrofolate reductase (dhfr) moiety]. previous work has shown that arg-dhfrts is long-lived at 23 degreesc but short-lived at 37 degrees ...	1999	9914499
a built-in arginine finger triggers the self-stimulatory gtpase-activating activity of rho family gtpases.	signal transduction through the rho family gtpases requires regulated cycling of the gtpases between the active gtp-bound state and the inactive gdp-bound state. rho family members containing an arginine residue at position 186 in the c-terminal polybasic region were found to possess a self-stimulatory gtpase-activating protein (gap) activity through homophilic interaction, resulting in significantly enhanced intrinsic gtpase activities. this arginine residue functions effectively as an "arginin ...	1999	9915787
cloning and characterization of prax-1. a new protein that specifically interacts with the peripheral benzodiazepine receptor.	using a cytoplasmic domain of the peripheral benzodiazepine receptor (pbr) as a bait in the yeast two-hybrid system, we have isolated a cdna encoding a new protein that specifically interacts with pbr. we named it prax-1, for peripheral benzodiazepine receptor-associated protein 1. prax-1 is a 1857-amino acid protein, the sequence of which was structurally unrelated to any known proteins. the gene encoding prax-1 is located in the q22-q23 region of the long arm of the human chromosome 17. the pr ...	1999	9915832
direct interaction of microtubule- and actin-based transport motors.	the microtubule network is thought to be used for long-range transport of cellular components in animal cells whereas the actin network is proposed to be used for short-range transport, although the mechanism(s) by which this transport is coordinated is poorly understood. for example, in sea urchins long-range ca2+-regulated transport of exocytotic vesicles requires a microtubule-based motor, whereas an actin-based motor is used for short-range transport. in neurons, microtubule-based kinesin mo ...	1999	9930703
cell cycle regulation of the double stranded rna activated protein kinase, pkr.	the interferon (ifn)-induced, double stranded rna (dsrna)-activated serine/threonine kinase, pkr, is a potent negative regulator of cell growth when overexpressed in yeast or mammalian cells. to determine whether endogenous pkr plays a role in cell growth control, we have investigated the regulation of pkr levels and activity during the cell cycle in human glioblastoma t98g cells. the steady-state level of pkr mrna in t98g cells was highest in growth arrested cells, dropped sharply within 3 h of ...	1999	9927188
physical delineation of a 700-kb region overlapping the looptail mutation on mouse chromosome 1.	the mouse looptail (lp) mutation is an established model for neural tube defects with homozygous lp embryos showing an open neural tube from the caudal midbrain to the tip of the tail. heterozygous lp mice are characterized by a "looped-tail" and wobbly head movements. the lp gene has been mapped to a 0.6-cm interval on mouse chromosome 1 delineated by two clusters of markers, fcer1gamma/usf1/d1mit113/d1wsu1 on the proximal side and fcer1alpha/spna1/d1mit149 distally. in the present study, we ha ...	1999	9933565
mammalian homologue of the caenorhabditis elegans unc-76 protein involved in axonal outgrowth is a protein kinase c zeta-interacting protein.	by the yeast two-hybrid screening of a rat brain cdna library with the regulatory domain of protein kinase c zeta (pkczeta) as a bait, we have cloned a gene coding for a novel pkczeta-interacting protein homologous to the caenorhabditis elegans unc-76 protein involved in axonal outgrowth and fasciculation. the protein designated fez1 (fasciculation and elongation protein zeta-1) consisting of 393 amino acid residues shows a high asp/glu content and contains several regions predicted to form amph ...	1999	9971736
the beta-glucan-binding lectin site of mouse cr3 (cd11b/cd18) and its function in generating a primed state of the receptor that mediates cytotoxic activation in response to ic3b-opsonized target cells.	mouse leukocyte cr3 (mac-1, alphambeta2 integrin) was shown to function as a receptor for beta-glucans in the same way as human cr3. soluble zymosan polysaccharide (szp) or pure beta-glucans labeled with fitc or 125i bound in a saturable and reversible manner to neutrophils, macrophages, and nk cells. this lectin activity was blocked by anti-cd11b mab m1/70 or 5c6 and did not occur with leukocytes from cr3-/- (cd11b-deficient) mice. szp preparations containing primarily mannose or glucose bound ...	1999	9973505
the histone acetylase pcaf is a phorbol-ester-inducible coactivator of the irf family that confers enhanced interferon responsiveness.	transcription factors of the interferon regulatory factor (irf) family bind to the type i interferon (ifn)-responsive element (isre) and activate transcription from ifn-inducible genes. to identify cofactors that associate with irf proteins, dna affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. the results demonstrated that the endogenous irfs bound to the isre are complexed with the histone acetylases, pcaf, gcn5, and p300/creb binding protein and ...	1999	10022868
mitop: database for mitochondria-related proteins, genes and diseases.	the mitop database http://websvr.mips.biochem.mpg. de/proj/medgen/mitop/ consolidates information on both nuclear- and mitochondrial-encoded genes and their proteins. the five species files- saccharomyces cerevisiae, mus musculus, caenorhabditis elegans, neurospora crassa and homo sapiens -include annotated data derived from a variety of online resources and the literature. a wide spectrum of search facilities is given in the interelated sections 'gene catalogues', 'protein catalogues', 'homolog ...	1999	9847163
alternative splicing results in differential expression, activity, and localization of the two forms of arginyl-trna-protein transferase, a component of the n-end rule pathway.	the n-end rule relates the in vivo half-life of a protein to the identity of its n-terminal residue. the underlying ubiquitin-dependent proteolytic system, called the n-end rule pathway, is organized hierarchically: n-terminal aspartate and glutamate (and also cysteine in metazoans) are secondary destabilizing residues, in that they function through their conjugation, by arginyl-trna-protein transferase (r-transferase), to arginine, a primary destabilizing residue. we isolated cdna encoding the ...	1999	9858543
vinexin: a novel vinculin-binding protein with multiple sh3 domains enhances actin cytoskeletal organization.	using the yeast two-hybrid system and an in vitro binding assay, we have identified a novel protein termed vinexin as a vinculin-binding protein. by northern blotting, we identified two types of vinexin mrna that were 3 and 2 kb in length. screening for full-length cdna clones and sequencing indicated that the two mrna encode 82- and 37-kd polypeptides termed vinexin alpha and beta, respectively. both forms of vinexin share a common carboxyl-terminal sequence containing three sh3 domains. the la ...	1999	9885244
molecular mapping of chromosome 2 deletions in murine radiation-induced aml localizes a putative tumor suppressor gene to a 1.0 cm region homologous to human chromosome segment 11p11-12.	radiation-induced acute myeloid leukemias (amls) in the mouse are characterized by chromosome 2 deletions. previous studies showed that a minimal deleted region (mdr) of approximately 6.5 cm is lost from one homologue in chromosome 2-deleted amls. an aml tumor suppressor gene is proposed to map within this mdr. in this study, we refine the mdr to a i cm interval between markers d2mit126 and d2mit185 by microsatellite analysis of 21 primary radiation-induced f i amls. the construction of a partia ...	1999	9885975
fog-2: a novel gata-family cofactor related to multitype zinc-finger proteins friend of gata-1 and u-shaped.	gata factors are transcriptional regulatory proteins that play critical roles in the differentiation of multiple cell types in both vertebrates and invertebrates. recent evidence suggests that the biological activities of both mammalian and drosophila gata factors are controlled in part by physical interaction with multitype zinc-finger proteins, friend of gata-1 (fog) and u-shaped (ush), respectively. here we describe a new fog-related polypeptide, designated fog-2, that is likely to participat ...	1999	9927674
localization of distant urogenital system-, central nervous system-, and endocardium-specific transcriptional regulatory elements in the gata-3 locus.	we found previously that neither a 6-kbp promoter fragment nor even a 120-kbp yeast artificial chromosome (yac) containing the whole gata-3 gene was sufficient to recapitulate its full transcription pattern during embryonic development in transgenic mice. in an attempt to further identify tissue-specific regulatory elements modulating the dynamic embryonic pattern of the gata-3 gene, we have examined the expression of two much larger (540- and 625-kbp) gata-3 yacs in transgenic animals. a lacz r ...	1999	9891089
zebrafish yac, bac, and pac genomic libraries.	numerous positional cloning projects directed at isolating genes responsible for the myriads of observed developmental defects in the zebrafish are anticipated in the very near future. in this chapter, we have reviewed the yac, bac, and pac large-insert genomic resources available to the zebrafish community. we have discussed how these resources are screened and used in a positional cloning scheme and have pointed out frequently formidable logistical considerations in the approach. despite being ...	1999	9891341
the suppressor of fused gene, involved in hedgehog signal transduction in drosophila, is conserved in mammals.	the suppressor of fused [su(fu)] gene of drosophila melanogaster encodes a protein containing a pest sequence [sequence enriched in proline (p), glutamic acid (e), serine (s) and threonine (t)] which acts as an antagonist to the serine-threonine kinase fused in hedgehog (hh) signal transduction during embryogenesis. the su(fu) gene isolated from a distantly related drosophila species, d. virilis, shows significantly high homology throughout its protein sequence with its d. melanogaster counterpa ...	1999	11252182
characterization of mouse ire1 alpha: cloning, mrna localization in the brain and functional analysis in a neural cell line.	in yeast, an endoplasmic reticulum (er)-associated protein, ire1p, is believed to initiate the unfolded protein response (upr), that is responsible for protein folding in the er under stressed conditions. two mammalian homologs of ire1p have been identified, ire1 alpha and ire1 beta. we have previously reported that familial alzheimer's disease linked presenilin-1 variants downregulate the signaling pathway of the upr by affecting the phosphorylation of ire1 alpha. in the present study, we clone ...	2000	11146108
n-linked oligosaccharide processing enzyme glucosidase ii produces 1,5-anhydrofructose as a side product.	alpha-1,4-glucan lyase cleaves alpha-1,4-linkages of nonreducing termini of alpha-1,4-glucans to produce 1,5-anhydrofructose (1,5-anfru). the enzymes isolated from fungi and algae show high homology with glycoside hydrolase family 31. purification of alpha-1,4-glucan lyase from rat liver using deae cellulose chromatography resulted in separation of two enzymatic active fractions, one was bound to the column and the other was in the flow-through. partial amino acid sequence determined from the ly ...	2000	11159920
characterization of sphingolipid long-chain base kinase in arabidopsis thaliana.	sphingolipid long-chain base (lcb) kinase catalyses the phosphorylation of sphingolipid lcb to form lcb 1-phosphate. based on sequence identity to a murine sphingosine kinase (murine sphk1a), we isolated and characterized a lcb kinase-like cdna in arabidopsis thaliana. the deduced amino acid sequence of the homologous cdna shows several regions that are highly conserved in lcb kinases from mouse, yeast, human and caenorhabditis elegans. these regions are not similar to those of other known kinas ...	2000	11171192
expression and catalytic activity of mouse leukotriene b4 omega-hydroxylase, cyp4f14.	we have isolated a cdna for a mouse leukotriene b4 omega-hydroxylase, cyp4f14. the cdna encoded a protein with 524 amino acids, whose sequence similarity is 95% that of rat cyp4f1. the microsomes from yeast cells transfected with cyp4f14 expression vector showed 0.1 nmol p450/mg protein and catalyzed omega-hydroxylations of leukotriene b4, 6-trans-leukotriene b4, lipoxin a4, prostaglandin a1, and several hydroxyeicosatetraeonic acids (hetes), with 8-hete being the most active substrate. in contr ...	2000	11185557
pathogenesis of prion diseases: a progress report.	almost 20 years have passed since stanley prusiner proposed that the agent causing transmissible spongiform encephalopathies consists exclusively of a protein and termed it prion. a mixed balance can be drawn from the enormous research efforts that have gone into prion research during this time. on the negative side, the protein-only hypothesis has not been conclusively proven yet. on the positive side, our understanding of spongiform encephalopathies has experienced tremendous advances, mostly ...	2000	11279534
mitop, the mitochondrial proteome database: 2000 update.	mitop (http://www.mips.biochem.mpg.de/proj/medgen/mitop/) is a comprehensive database for genetic and functional information on both nuclear- and mitochondrial-encoded proteins and their genes. the five species files--saccharomyces cerevisiae, mus musculus, caenorhabditis elegans, neurospora crassa and homo sapiens--include annotated data derived from a variety of online resources and the literature. a wide spectrum of search facilities is given in the overlapping sections 'gene catalogues', 'pr ...	2000	10592209
molecular characterization of kex1, a kexin-like protease in mouse pneumocystis carinii.	expression screening of a pneumocystis carinii-infected mouse lung cdna library with specific monoclonal antibodies (mabs) led to the identification of a p. carinii cdna with extensive homology to subtilisin-like proteases, particularly fungal kexins and mammalian prohormone convertases. the 3.1 kb cdna contains a single open reading frame encoding 1011 amino acids. structural similarities to fungal kexins in the deduced primary amino acid sequence include a putative proenzyme domain delineated ...	2000	10721706
comparison of nucleosome remodeling by the yeast transcription factor pho4 and the glucocorticoid receptor.	chromatin reorganization of the pho5 and murine mammary tumor virus (mmtv) promoters is triggered by binding of either pho4 or the glucocorticoid receptor (gr), respectively. in order to compare the ability of pho4 and gr to remodel chromatin and activate transcription, hybrid promoter constructs were created by insertion of the mmtv b nucleosome sequence into the pho5 promoter and then transformed into a yeast strain expressing gr. activation of either pho4 (by phosphate depletion) or gr (by ho ...	2000	10722753
yra1p, a conserved nuclear rna-binding protein, interacts directly with mex67p and is required for mrna export.	mex67p and mtr2p constitute an essential mrna export complex that interacts with poly(a)+ rna and nuclear pore proteins. we have identified yra1p, an intranuclear protein with in vitro rna-rna annealing activity, which directly binds to mex67p. the complex between yra1p and mex67p was reconstituted in vitro and shown by uv-crosslinking to bind directly to rna. mutants of yra1 are impaired in nuclear poly(a)+ rna export at restrictive growth conditions. aly, the mouse homologue of yra1p and a tra ...	2000	10722314
rab geranylgeranyl transferase alpha mutation in the gunmetal mouse reduces rab prenylation and platelet synthesis.	few molecular events important to platelet biogenesis have been identified. mice homozygous for the spontaneous, recessive mutation gunmetal (gm) have prolonged bleeding, thrombocytopenia, and reduced platelet alpha- and delta-granule contents. here we show by positional cloning that gm results from a g-->a substitution mutation in a splice acceptor site within the alpha-subunit of rab geranylgeranyl transferase (rabggta), an enzyme that attaches geranylgeranyl groups to rab proteins. most rabgg ...	2000	10737774