Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
genomic relationship of porcine hemagglutinating encephalomyelitis virus to bovine coronavirus and human coronavirus oc43 as studied by the use of bovine coronavirus s gene-specific probes.the genomic relationship of porcine hemagglutinating encephalomyelitis virus (hev) to bovine coronavirus (bcv) and human coronavirus (hcv) strain oc43 was examined by dot blot hybridization assays. two bcv s gene-specific probes were generated by polymerase chain reaction from the avirulent l9-strain of bcv. probes were located in the s1 and the s2 region of the peplomeric (s) glycoprotein gene. the s1 probe (726 bp) hybridized with bcv and hcv-oc43, but not with hev under moderate stringency hy ...19957646353
[detection of hepatitis c virus rna and c33c antigen in the liver tissue from hepatitis c virus infection patients with chronic liver disease].to study the distribution of hepatitis c virus (hcv) in liver tissue and its pathogenesis, nonisotopic in situ hybridization with a digoxin labelled cdna probe to 5'-nc region of hcv and immunohistochemical study with monoclonal anti-body of anti hcv c33c were performed on the liver tissue from 24 hcv infection patients with chronic liver disease. the results were as follows: in the liver tissue of 17 patients with hcv infection (both elisa anti-hcv and nested pcr hcv rna positive in serum), the ...19957648939
hepatitis c virus within a malignant lymphoma lesion in the course of type ii mixed cryoglobulinemia.hepatitis c virus (hcv) has been implicated as the major etiologic factor sustaining b-cell clonal expansion in type ii mixed cryoglobulinemia (mc). a putative pathogenetic role of hcv in the development of mc-associated b-cell malignancies has also been speculated. we report for the first time the localization of hcv within a parotid non-hodgkin's lymphoma (nhl) lesion in the course of hcv-related type ii essential mc, an important step to implicate any infectious agent in the lymphomagenesis. ...19957655017
interaction of polypyrimidine tract-binding protein with the 5' noncoding region of the hepatitis c virus rna genome and its functional requirement in internal initiation of translation.initiation of translation of the human hepatitis c virus (hcv) rna genome occurs by internal ribosome entry into the 5' noncoding region (5'ncr) in a cap-independent manner. the internal ribosome entry site of the hcv 5'ncr has been previously defined to encompass almost the entire 5'ncr. here we report the interaction of polypyrimidine tract-binding protein (ptb) at three distinct regions within the 5'ncr by uv cross-linking assays. all three regions contain a consensus polypyrimidine tract mot ...19957666538
prevalence of hepatitis b, hepatitis c, and human immunodeficiency virus markers among hospital employment applicants.to delineate baseline seroprevalence rates before job placement, applicants for employment (n = 300) at a large urban medical center were screened for serologic markers to the hepatitis b virus (hbv), hepatitis c virus (hcv), and human immunodeficiency virus (hiv) during a 15-week period in 1992. eighteen applicants (6%) were positive for antibodies to hbv, nine (3%) for hcv, and 3 (1%) for hiv. there was no association by gender for any of these viral markers; however, both hbv and hcv were sig ...19957670906
sexual transmission of hepatitis c virus among patients attending sexually transmitted diseases clinics in baltimore--an analysis of 309 sex partnerships.the prevalence of antibodies to hepatitis c virus (anti-hcv), the behavioral and laboratory-derived risk factors for anti-hcv, and the quantity and homology of hcv rna were assessed among 1039 non-injection drug-using sexually transmitted disease (std) patients representing 309 sex partnerships. thirty-seven (7%) of 555 males and 19 (4%) of 484 females had anti-hcv. in logistic regression analyses, factors associated with anti-hcv included age (p < .001), greater numbers of lifetime sex partners ...19957535827
evaluation of a multiple peptide assay for typing of antibodies to the hepatitis c virus: relation to genomic typing by the polymerase chain reaction.a panel of 16 type-specific synthetic peptides corresponding to variable antigenic regions within the hepatitis c virus (hcv) core, nonstructural 4 (ns4), and ns5 proteins was synthesised. the peptide panel was used to develop an enzyme immunoassay (eia) for the detection of antibodies directed to hcv type 1 (genotypes i/1a and ii/1b), type 2 (genotypes iii/2a and iv/2b), and type 3 (genotype v/3). the peptides corresponded to residues 68-81 of the hcv core (types 1, 2, and 3), residues 1692-170 ...19957536231
is the detection of anti-hepatitis c virus core igm influenced by the presence of serum rheumatoid factor?rheumatoid factor (rf) induces false-positive results in the detection of serum antibodies, especially of the igm type. about 70% of the patients with chronic hepatitis c have abnormal levels of serum rf. the aim of this study was to determine whether the presence of serum rf could influence the detection of anti-hcv core igm, using an assay designed not to pick up rfs by the addition of goat antibodies directed against human igg in the sample diluent. serum anti-hcv core igm antibodies and rf w ...19957536232
[medical treatment of chronic viral hepatitis].alpha-interferon and vidarabine monophosphate are currently available for the treatment of chronic active hepatitis b. treatment is indicated in patients with high transaminase levels, evidence of replicating virus and histological inflammation. interferon or vidarabine suppress viral replication and normalize transaminase activity in 30 to 40% of the patients. the main predictive factors of response to therapy are a high transaminase level, a low replication level, and the absence of immunodepr ...19957536954
hepatitis b, c, and d virus infections in patients with chronic hepatitis, cirrhosis, and hepatocellular carcinoma: a comparative study in niger.eighty-nine sahelian african patients with chronic active hepatitis (cah) (14), cirrhosis (49), hepatocellular carcinoma (hcc) (26), and 47 controls were tested for hepatitis b virus (hbv, hepatitis b surface antigen [hbsag]) and hepatitis d virus (hdv, anti-hdv antibody). seventy-three percent of the patients were positive for hbsag versus 29.8% of the controls (p < 0.0001). with anti-hdv test, 55.0% of the patients were positive versus 17.0% of the controls (p < 0.0001). to assess the prevalen ...19957537942
antibodies in human sera specific to hypervariable region 1 of hepatitis c virus can block viral attachment.it has been postulated that antibodies specific to the hypervariable region 1 (hvr1) within the putative envelop protein e2 of hepatitis c virus (hcv) can neutralize virus. we studied such antibodies in sera of patients who were infected in a single-source outbreak by a contaminated anti-d immunoglobulin preparation (hcv-ad78). the nucleotide sequences of cdnas encoding hvr1 of hcv-ad78 were determined. the four major variants (hvr1.a, b, c, and d) were expressed as fusion proteins in escherichi ...19957538251
multiple false reactions in viral antibody screening assays after influenza vaccination.in december 1991, us blood centers reported an unusual increase in donations that tested falsely reactive for antibodies to two or more (multiple false positive) of the following viruses: human immunodeficiency virus type 1 (hiv-1), human t-cell lymphotrophic virus type i (htlv-i), and hepatitis c virus. many of these donations were from people who had recently received the 1991-1992 influenza vaccine, raising the possibility that this vaccine had somehow specifically caused the problem of multi ...19957539579
[hepatitis-c virus antibody conversion in 3 hemodialysis patients in various dialysis departments].in three haemodialysis patients, two women of 72 and 35 years and a man of 65 years, who were dialysed in three different dialysis departments, recent seroconversion of hepatitis c was observed. the source of the infection and the transmission route remained unknown.19957540730
a molecular sensor system based on genetically engineered alkaline phosphatase.binding and signaling proteins based on escherichia coli alkaline phosphatase (ap; ec 3.1.3.1) were designed for the detection of antibodies. hybrid proteins were constructed by using wild-type ap and point mutants of ap [asp-101 --> ser (d101s) and asp-153 --> gly (d153g)]. the binding function of the hybrid proteins is provided by a peptide epitope inserted between amino acids 407 and 408 in ap. binding of anti-epitope antibodies to the hybrid proteins modulates the enzyme activity of the hybr ...19957541135
[heterogeneity of antimicrosomal autoantibodies in chronic hepatitis c virus infection and delta hepatitis].microsomal antigen autoantibodies are typical of type 2 autoimmune hepatitis, and a strong association with chronic hepatitis c virus (hcv) infection has been reported in certain geographical areas. these autoantibodies have been denominated lkm-1 to differentiate them from those associated with thienylic acid-induced hepatitis (lkm-2) and from those seen in patients with chronic delta hepatitis (lkm-3). to investigate the antigenic specificity of autoantibodies associated with chronic hepatitis ...19957542466
hepatitis c virus infection among chronic dialysis patients in the south of france: a collaborative study.during the last quarter of 1992, 984 patients from 13 dialysis centers in the provence-alpes-côte-d'azur region in france participated in a multicenter cross-sectional study to determine the prevalence, the risk factors, and the clinical consequences of infection by the hepatitis c virus (hcv). serum samples were tested for anti-hcv antibodies using second-generation enzyme-linked immunosorbent assay (elisa). in the case of a positive result, a combination test was performed using second-generat ...19957532916
hepatitis c virus core protein: synthesis, affinity purification and immunoreactivity with infected human sera.the genomic region encoding the core (c) protein (amino acids 1-162) of hepatitis c virus (hcv) was expressed in escherichia coli as a recombinant (re-) protein with the maltose-binding protein (mbp) using the prokaryotic expression vector pmal-cr1. the fusion protein (c::mbp) was identified as a approx. 62-kda polypeptide by immunoblot analysis using antiserum to mbp and hcv-infected human sera. the size of c::mbp corresponded to the calculated combined molecular mass of the approx. 20-kda hcv ...19957533115
hepatocellular codistribution of c100, c33, c22, and ns5 hepatitis c virus antigens detected by using immunopurified polyclonal spontaneous human antibodies.hepatitis c virus (hcv) antigens in liver biopsy have been detected by immunohistochemistry using both spontaneous human igg and murine monoclonal or rabbit polyclonal monospecific reagents. conflicting results have been obtained in different studies. this was probably because of the incapacity of single experimental antibodies, raised against synthetic or recombinant peptides, to recognize native tissue antigens. to overcome this possibility, we immunopurified monospecific spontaneous polyclona ...19957533125
ctl responses of hla-a2.1-transgenic mice specific for hepatitis c viral peptides predict epitopes for ctl of humans carrying hla-a2.1.vaccine development in animal models depends on ability to recognize epitopes seen by human t cells. in this work, we show that ctl responses in transgenic mice expressing human hla-a2.1 prospectively predict the same four of 11 hepatitis c virus (hcv) structural protein-derived peptides, expressing a sequence motif for hla-a2.1 binding, that are actually recognized by human a2.1-restricted ctls. the ctls also recognized targets endogenously expressing these proteins. human ctls from hcv-infecte ...19957533182
prevalence of hepatitis b, hepatitis c, and human immunodeficiency virus infection among women attending prenatal clinics in san juan, puerto rico, from 1989-1990.to evaluate the prevalence of hepatitis b virus (hbv), hepatitis c virus (hcv), and human immunodeficiency virus (hiv) among pregnant women in puerto rico.19957528370
molecular epidemiology of hepatitis c virus infection amongst intravenous drug users in rural communities.the prevalence of hepatitis c virus (hcv) infection amongst a group of intravenous drug users (ivdus) resident in west suffolk (east anglia, england) was investigated and compared with the prevalence of infection with hepatitis b virus (hbv) and human immunodeficiency virus (hiv). in addition, both the level of hcv persistence, as defined by detection of viral rna, and the hcv genotypes present in this population were determined. it was found that hcv antibodies were present in 59% of those test ...19957542693
human monoclonal antibodies for the immunological characterization of a highly conserved protein domain of the hepatitis c virus glycoprotein e1.although both envelope glycoproteins of the hepatitis c virus, e1 and e2/ns1, show a high degree of sequence variation, the e1 protein includes a well conserved domain, which may be functionally important. we have analysed the human b cell response to a peptide fragment from amino acid residues 314-330 (ep3) covering the central conserved sequence of this domain. anti-hepatitis c virus-positive blood donors were screened for anti-ep3 antibodies with an elisa based on immobilized peptide. thirty ...19957544250
a helper t-cell antigen enhances generation of hepatitis c virus-specific cytotoxic t lymphocytes in vitro.a t-cell helper for generation of hepatitis c virus-specific cytotoxic t lymphocytes was studied in three patients with chronic hepatitis c. in all three, human leukocyte antigen b44-restricted cytotoxic t lymphocytes recognizing an epitope in hepatitis c virus nucleocapsid protein residues 81-100 were generated from the peripheral blood lymphocytes by repeated stimulation with a synthetic hepatitis c virus nucleocapsid peptide. the proliferative response of peripheral blood lymphocytes to hepat ...19957545209
treatment of chronic hepatitis c with cirrhosis with recombinant human granulocyte colony-stimulating factor plus recombinant interferon-alpha.interferon therapy in cirrhotic patients with hepatitis c virus infection is not efficient. in an attempt to improve the response rate, a pilot study using recombinant human granulocyte colony-stimulating factor (rhg-csf) alone, and in combination with recombinant interferon-alpha (rifn), was carried out. fifteen cirrhotic patients with hepatitis c virus infection were randomly allocated into 3 groups to receive treatment: 0.5, 1, or 1.5 micrograms/kg of rhg-csf daily for 4 weeks, followed by a ...19957545214
relation between markers for viral hepatitis and clinical features of japanese patients with hepatocellular carcinoma: possible role of alcohol in promoting carcinogenesis.to assess the relationship between hepatitis virus markers and the clinical features of hepatocellular carcinoma (hcc), we measured markers for hepatitis b virus (hbv) and hepatitis c virus (hcv) in 88 japanese patients with hcc. twelve (14%) patients were hbsag-positive and 67 (76%) were anti-hcv-positive (both c100-3 and c11/c7). hcv-rna was detected in 8 (38%) of the 21 anti-hcv-negative patients by pcr, so that 75 patients (85%) were infected with hcv. of the hbsag-negative patients infected ...19957545640
clinical and histopathologic analysis of the relationship between lichen planus and chronic hepatitis c.a prospective clinical investigation of 45 patients with lichen planus (lp) demonstrated a significant association between lp and chronic hepatitis c. anti-hepatitis c virus (hcv) antibodies were found in 17 (37.8%) of the 45 lp patients. this was significantly higher than in the controls. this higher prevalence of anti-hcv antibodies was found equally in both male and female patients in the three types of lp; cutaneous only type, mucous only type, and both cutaneous and mucous type. most of the ...19957545708
hepatitis c virus infection and genotypes in japanese hemophiliacs.liver function and antibodies to hepatitis c virus and to human immunodeficiency virus-1 were examined in 195 japanese patients with hemophilia. one hundred and seventy-three were positive for antibody to hcv and 61 for antibody to human immunodeficiency virus-1. in 63 patients, we examined hcv genotypes according to the double polymerase chain reaction method. forty cases (63%) were infected with hepatitis c virus with a single genotype, including type 1a in five, type 1b in 21, type 2a in seve ...19957545774
non-muscle myosin as target antigen for human autoantibodies in patients with hepatitis c virus-associated chronic liver diseases.three patients with hepatitis c virus (hcv)-related chronic liver disease were shown to have autoantibodies strongly reacting with cytoskeletal fibres of non-muscle cells. the heavy chain of non-muscle myosin microfilament was the main target for those autoantibodies, as determined by (i) cell and tissue immunofluorescence studies showing colocalization with an anti-myosin antibody prototype; (ii) primary reactivity in immunoblotting with a 200-kd protein, using either molt-4 cells, human platel ...19957697925
failure to incriminate hepatitis b, hepatitis c, and hepatitis e viruses in the aetiology of fulminant non-a non-b hepatitis.sporadic non-a, non-b hepatitis is the most common indication for liver transplantation in patients presenting with fulminant and subacute liver failure. this study used serological, histological, and molecular biological techniques to examine specimens from 23 consecutive patients transplanted for sporadic non-a, non-b hepatitis. no evidence was found of hepatitis c virus, hepatitis e virus, or 'cryptic' hepatitis b virus infection.19957698704
should patients with human immunodeficiency virus infection or chronic hepatitis donate blood for autologous use?the purpose of this project is to formally evaluate the benefits and the risks of allowing patients with human immunodeficiency virus (hiv) infection or hepatitis to donate blood for autologous use.19957701551
patients, needles, and health care workers.the risk of transmission of blood-borne pathogens in the health care environment is determined by three main factors: the nature and frequency of exposure to blood or body fluids, the risk of transmission of infection after a single exposure to the pathogen, and the prevalence of infected and susceptible patients and health care workers. in this article, all three parts of the risk equation are examined and brought into focus by addressing the risks and pathologic consequences of infection with ...19957707172
long-term serologic follow-up of hepatitis c virus-seropositive homosexual men.hepatitis c virus (hcv) infection may go undiagnosed and continue to present a source of community-acquired or transfusion-associated infection because of shortcomings in sensitivity, specificity, and reproducibility of serologic tests. this project was designed to longitudinally study persons who were hcv seropositive or were at risk for seroconversion to characterize the course of infection. sequential serum samples obtained semiannually from 617 homosexual male volunteers were available for s ...19957697532
increased prevalence of htlv-i infection in patients with hepatocellular carcinoma associated with hepatitis c virus.the progression from chronic hepatitis c virus (hcv) infection to hepatocellular carcinoma (hcc) has been reported. we evaluated whether co-infection with the human t-lymphotropic virus type i (htlv-i) might be associated with this transition in a cross-sectional analysis of 127 patients with hcv-chronic hepatitis (mean age = 51.7) and 43 patients with hcv-associated hcc (mean age = 62.4); the seroprevalence of anti-htlv-i was 9.5% and 30.2%, respectively. for subjects 50 years or older, the ser ...19957737899
clinical outcomes after transfusion-associated hepatitis c.the extent of serious complications in people who have acquired chronic hepatitis c infection after a blood transfusion is unclear.19957739682
[treatment of chronic virus hepatitis with acetylsalicylic acid].the treatment of chronic hepatitis b and c with recombinant interferon (ifn) has only poor durable response rates. in the past only the lack of any prior effective therapy regimen could justify the use of this expensive agent. dose escalating or prolonged treatment courses did not enhance the rate of sustained remissions. pre- or cotreatment with antiviral (e.g. acyclovir, ribavirin, isoprenosin) or immunomodulating (e.g. prednisone, gamma ifn) drugs have not influenced the resistance to exogeno ...19957740291
human immunodeficiency virus infection and porphyria cutanea tarda: coexistence of risk factors or causative association?several recent reports have described an association between human immunodeficiency virus (hiv) infection and porphyria cutanea tarda (pct). we describe two hiv-infected patients who developed pct and review 29 other reported cases of coexistent pct and infection due to hiv. recognized porphyrinogenic factors were identified in our patients and in 27 of the 29 cases described in the literature. even in the two cases that lacked obvious precipitant factors for pct, the possibility of hepatitis c ...19957742441
closed video transurethral resection of the prostate for the prevention of infection.an instrument with which safe and clean video transurethral resection of the prostate (video turp) can be performed has been developed by the author; its use could minimize the risk to the operating staff of being infected by such dangerous viruses as human immunodeficiency virus (hiv), hepatitis b virus (hbv), and hepatitis c virus (hcv). the instrument consists of a bag made of transparent synthetic resin (polyvinyl chloride), used to cover the patient's penis and a resectoscope with a super m ...19957744138
arsphenamine jaundice and the recognition of instrument-borne virus infection.soon after its introduction in 1910, intravenous arsphenamine treatment for syphilis was found to be complicated by jaundice. the underlying cause, unsterile syringes and needles, was eventually recognised in the early 1940s, mainly through the efforts of british army investigators. the infection most often transmitted was probably hepatitis b virus (hbv), but the high mortality in a few of the outbreaks of arsphenamine jaundice suggests that variants of hbv, or other hepatitis viruses, were som ...19957744400
successful cyclophosphamide treatment of cryoglobulinemic membranoproliferative glomerulonephritis associated with hepatitis c virus infection.a 54-year-old man with cryoglobulinemia and chronic hepatitis c infection presented with progressive renal insufficiency caused by membranoproliferative glomerulonephritis. because of a steady decline in renal function, cyclophosphamide therapy was instituted. within 1 month of starting therapy, his cryoglobulins disappeared, and in 3 months, his creatinine clearance had improved from 56 ml/min to 89 ml/min. at no point in his course was there clinical evidence of liver disease. after 1 year, cy ...19957747735
treatment and prevention of chronic viral hepatitis.chronic viral hepatitis caused by hepatitis b, c or d may lead to cirrhosis, hepatocellular failure and hepatocellular carcinoma. the morbidity of these diseases has necessitated a prolonged search for effective therapy. interferon-alpha has been studied widely, and remains the mainstay of treatment. therapy for hepatitis b has now become possible with the demonstration that alpha-interferons inhibit hepatitis b virus (hbv) replication and that prolonged therapy can lead to a remission in diseas ...19957716182
prevalence and incidence of hepatitis b virus, hepatitis c virus and human immunodeficiency virus among personnel and patients of a psychiatric hospital.in 1989 and 1992, hiv, hbv and hcv serosurveys were carried out among personnel and patients of an italian psychiatric hospital. no hiv cases were found. mean annual hbv seroconversion rate was 0.99% in patients and 4.4% in personnel; hcv rate was 0.42% among patients. although the nosocomial risk of bloodborne infection was low, efforts should be made to minimize it.19957672084
prevalence of hepatitis b, hepatitis c and human immune deficiency virus markers among patients with hereditary haemolytic anaemias.to determine the prevalence of hepatitis b virus (hbv), hepatitis c virus (hcv) and human immunodeficiency virus (hiv) markers among bahraini children with hereditary haemolytic anaemias, a cross-sectional study was conducted at the paediatric outpatient clinic of sulimaniya medical center in the state of bahrain. a total of 242 patients with hereditary haemolytic anaemias were enrolled in the study: 171 (71%) with sickle cell syndromes, 59 (24%) with beta thalassaemia major and 12 (5%) with alp ...19957677412
hepatitis c virus in breast milk. 19957707835
[methods of study of the hepatitis c virus genome. diagnostic tools in human pathology].the study of the molecular biology of infectious agents involves the examination of their genomes and the products of those genomes. molecular biology methods may therefore allow us to study either dnas or rnas. the study of genomic rnas (viruses) or messenger rnas (all infectious agents) is used increasingly in infectious disease pathology. the hepatitis c virus was identified in 1989 and was shown to be responsible for a large number of chronic hepatitis cases in france and worldwide. this vir ...19957711774
unbiased usage of t-cell receptor beta variable region genes in peripheral blood cells of hepatitis c patients: no correlation with superantigen effect.hepatitis c virus (hcv) infection frequently causes chronic hepatitis and lack of virus clearance in these patients. in addition, many patients infected by hcv also present with hypergammaglobulinemia in the early stage of chronic infection. these observations raise a possible viral superantigen effect induced by hcv, because viral superantigen found in human immunodeficiency virus (hiv) or in replication of defective murine leukemia virus (mulv) is associated with t-cell dysfunction and polyclo ...19957714489
clinical recurrence of hepatitis a following liver transplantation for acute liver failure.this paper documents clinically significant recurrence of hepatitis a virus (hav) infection in a 63-year-old man transplanted for hav-related acute liver failure. hav rna was documented in the explant and, following early clearance from the blood and graft, was again detected in post-operative biopsies at the time of an acute hepatic illness. although the clinical and biochemical abnormalities resolved completely, the patient had a second episode of graft dysfunction 6 months later and investiga ...19957714491
detection of hepatitis c virus rna in the cell fraction of saliva before and after oral surgery.the presence of hepatitis c virus (hcv) rna in serum, whole saliva, and saliva from the submaxillary glands was investigated before and after oral surgery. the presence of hcv rna (positive and negative-strand rna) was determined in serum and saliva by a nested polymerase chain reaction in 26 anti-hcv positive patients, of whom 11 were coinfected with human immunodeficiency virus-1. oral surgery was carried out on five occasions on four of the patients. hcv rna was detected in the sera of 23 of ...19957775943
a novel method for analysis of viral proteinase activity encoded by hepatitis c virus in cultured cells.we developed a novel method for analysis of hepatitis c viral proteinase activity in cultured cells, in which the proteinase activity was measured as the enhancement of reporter gene expression. in this system, plasmids encoding a reporter gene, the enzyme gene, and the substrate gene were simultaneously transfected into cos-1 cells. the reporter plasmid contains chloramphenicol acetyltransferase (cat) gene downstream of an enhancer/promoter sequence derived from the human t-cell leukemia virus ...19957778761
etiology of acute sporadic hepatitis in adults in senegal and tunisia.markers for acute hepatitis a, b, c and e virus infections were examined in the sera of 72 patients suffering from acute hepatitis in senegal and tunisia. hepatitis b was responsible for 36% and hepatitis c for 21% of the cases. acute hepatitis a was not diagnosed. hev infection was not observed in senegal and represents only 4% of the acute hepatitis cases in tunisia.19957784826
orthotopic liver transplantation in a patient with severe hemophilia a: a life-saving treatment for the first italian case.clinical cure of hemophilia a by orthotopic liver transplantation has been reported in 11 cases. we describe the first successful italian case. a 27-year-old man had cirrhosis caused by previous infections with the hepatitis b, c and d viruses following life-long treatment with factor viii concentrates made from large plasma pools. he was, however, seronegative for the human immunodeficiency virus. in the year before transplantation, life-threatening gastrointestinal bleeding due to severe esoph ...19957787210
evidence of non-a, non-b, non-c infection in chronic hepatitis by polymerase chain reaction testing for hepatitis b and c viruses.although hepatitis c virus is clearly the major cause of non-a, non-b acute and chronic hepatitis, there is a group of patients with histologically documented chronic hepatitis with no serological marker of hepatitis b and c, nor any other risk factors for liver disease.19957790699
haemophilia a management in victorian, new south wales and south australian haemophilia centres.to examine the management of haemophilia a in australia and to compare it with international trends.19957791641
[initial studies of the prevalence of human herpesvirus 7 (hhv-7) in mexican blood donors].human herpesvirus 7 (hhv-7) has been recently isolated from cd4-positive peripheral blood lymphocytes of a healthy person. the present study was performed to find the antibody prevalence of this virus in the healthy mexican population. two hundred blood samples from candidates for blood donation at the general hospital of mexico were studied with the indirect immunofluorescence test (ifa) in hhv-7 infected supt1 cells. 83.5% were male persons and 16.5% female, the mean age for the male group was ...19957792272
[progress in hepatitis diagnosis].until today only five pathogens of viral hepatitis have been discovered: hepatitis virus a (hav), b (hbv), c (hcv), d (hdv) and e (hev). an update on the state of the art of actually established diagnostic procedures is briefly summarized. some recent topics and problems are covered more extensively; in the case of hbv the value of hbsag concentration as a prognostic marker, the finding 'anti-hbc only' the role of hbv mutants, the determination of circulating hbv genomic material, and modalities ...19957792463
liver cytosolic 1 antigen-antibody system in type 2 autoimmune hepatitis and hepatitis c virus infection.within the multiform liver/kidney microsomal (lkm) family, a subgroup of sera that reacts with a liver cytosolic (lc) protein has been isolated and the new antigen-antibody system is called lc1. unlike lkm antibody type 1 (anti-lkm1), anti-lc1 is said to be unrelated to hepatitis c virus (hcv) infection and has therefore been proposed as a marker of 'true' autoimmune hepatitis type 2. altogether 100 lkm1 positive sera were tested by immunodiffusion (id). twenty five gave a precipitation line wit ...19957797126
variants of hepatitis b, c and d viruses: molecular biology and clinical significance.variants of hepatitis b virus (hbv), hepatitis c virus (hcv) and of the hepatitis delta virus (hdv) have been identified in patients both with acute and chronic infections. in the hbv dna genome, naturally occurring mutations have been found in all viral genes, most notably in the genes coding for the structural envelope and nucleocapsid proteins. in the hcv rna genome, the regions coding for the structural envelope proteins 1 and 2 as well as the 3'-contiguous nonstructural region 1 were found ...19957750674
identification of two flavivirus-like genomes in the gb hepatitis agent.a subtractive pcr methodology known as representational difference analysis was used to clone specific nucleotide sequences present in the infectious plasma from a tamarin infected with the gb hepatitis agent. eleven unique clones were identified, seven of which were examined extensively. all seven clones appeared to be derived from sequences exogenous to the genomes of humans, tamarins, saccharomyces cerevisiae, and escherichia coli. in addition, sequences from these clones were not detected in ...19957724574
[hepatitis viruses].five different hepatitis viruses that cause acute and chronic hepatitis in human beings have been identified. hepatitis viruses are designated by the letters a, b, c, d and e and abbreviated as hav, hbv, hcv, hdv, and hev, respectively. although each belongs to a distinctly different virologic group, they share the feature of hepatotropism, which remains poorly understood. hav and hev are transmitted through a fecal-oral route. hdv, also referred to as the delta agent, is a defective, parenteral ...19957725016
[serum biological markers and screening of viral hepatitis].the diagnosis of hepatitis a is based on igm anti-hav positivity. igg anti-hav indicates immunity from further infections of hepatitis a. in acute hepatitis b, hbs ag is the first marker to appear in the serum, followed by hbe ag and anti-hbc. after recovery, hbs ag disappears, anti-hbs appears with anti-hbe and anti-hbc and persists for many years. anti-hbs is indicative of recovery and immunity. chronic hepatitis b infection is defined as the persistence of hbs ag for more than 6 months. the p ...19957725017
[extra-hepatic manifestations of viral hepatitis].a variety of prodromal symptoms of viral hepatitis (urticaria, fever, arthralgias, headache, polyradiculonevritis) are attributed to a, b, c, d or e hepatitis only when jaundice appears, and because they disappear with it. spectacular extrahepatic symptoms (polyarteritis nodosa, cryoglobulinemia, glomerulonephritis, marrow aplasia...) may be associated with b or c hepatitis without any liver symptom. some of the extrahepatic symptoms observed during chronic hepatitis c therapy with interferon (t ...19957725020
characterization of an established human hepatoma cell line constitutively expressing non-structural proteins of hepatitis c virus by transfection of viral cdna.a human hepatoma cell line constitutively expressing proteins of hepatitis c virus (hcv) was established by transfection with cdna encoding part of the virus nonstructural (ns) genome region. proteins consistent with authentic processing at ns3/ns4a, ns4a/ns4b and ns4b/ns5a were identified. pulse-chase experiments indicated that the cleavage between ns3 and ns4a occurred first and cleavage at other sites followed. expression of specific surface antigens in response to the presence of hcv protein ...19957730805
low risk of viral infection after administration of vapor-heated factor vii concentrate or factor ix complex in first-time recipients of blood components. international factor safety study group.vapor-heated human factor vii concentrate and human factor ix complex are both obtained from prothrombin complex, undergo similar methods of manufacture, and are subjected to an identical two-step vapor-heating process for virus inactivation.19957878711
reduction in transmission of hepatitis c after the introduction of a heat-treatment step in the production of c1-inhibitor concentrate.the transmission of viral infections via protein concentrates made from a large pool of plasma depends on the selection of donors, fractionation process, and virucidal methods. to date, no data are available on the infectivity risk of plasma concentrates of the inhibitor of the first component of complement (c1-inh).19957878712
human t-lymphotropic virus type i and type ii infections and correlation with risk factors in blood donors from são paulo, brazil.little is known about the prevalence of and risk factors for human t-lymphotropic virus type i and type ii (htlv-i, htlv-ii) infections in brazil.19957878720
infectious disease markers in blood donors in northern thailand.a major epidemic of human immunodeficiency virus type 1 (hiv-1) infections that are primarily due to heterosexual transmission has developed in thailand since 1988. the epidemic has been most severe in northern thailand. the blood banks in chiang mai began screening donors for hiv-1 antibodies in february 1988 and for p24 antigen in april 1992.19957878721
interleukin-8 and granulocyte-macrophage colony-stimulating factor secretion in hepatocellular carcinoma and viral chronic active hepatitis.interleukin-8 (il-8) and granulocyte-macrophage colony-stimulating factor (gm-csf) are important mediators of inflammation and immune response in human disease. to demonstrate their importance in pathophysiological processes in liver disease, we measured the circulating levels of il-8 and gm-csf in patients with hepatocellular carcinoma (hcc) and chronic active hepatitis (cah). il-8 and gm-csf levels in serum samples were determined with highly specific and sensitive enzyme-linked immunosorbent ...19957859412
cytotoxic t lymphocyte response to hepatitis c virus-derived peptides containing the hla a2.1 binding motif.the hla class i-restricted cytotoxic t lymphocyte (ctl) response is a major defense mechanism in viral infections. it has been suggested that the ctl response may contribute to viral clearance and liver cell injury during hepatitis c virus (hcv) infection. to test this hypothesis requires an understanding of the characteristics of hcv-specific cytotoxic effector cells and identification of the target antigens to which they respond. to begin this process we stimulated peripheral blood mononuclear ...19957860734
human immunodeficiency virus infection as risk factor for mother-to-child hepatitis c virus transmission; persistence of anti-hepatitis c virus in children is associated with the mother's anti-hepatitis c virus immunoblotting pattern.to determine the rate of vertical transmission of hepatitis c virus (hcv), we prospectively studied 45 babies born to anti-hcv-positive women with or without concomitant infection with the human immunodeficiency virus (hiv). we performed a second-generation recombinant immunoblotting assay, alanine transaminase (alt) evaluation, and hcv-rna testing on sera from 27 infants of hcv+, hiv- mothers and 18 babies of hcv+, hiv+ women, at birth and thereafter. after birth, hcv antibodies progressively d ...19957843701
complete sequence (20 kilobases) of the polyprotein-encoding gene 1 of transmissible gastroenteritis virus.the entire nucleotide sequence of cloned cdnas containing the 5'-untranslated region and gene 1 of purdue-115 strain of transmissible gastroenteritis virus (tgev) was determined. this completes the sequence of the tgev genome, which is 28,579 nucleotides long. the gene 1 is composed of two large open reading frames, orf1a and orf1b, which contain 4017 and 2698 codons, respectively (stop excluded). a brief, three-codon-long orf is present upstream of orf1a. orf1b overlaps orf1a by 43 bases in the ...19957856095
eradication of hepatitis c virus after interferon-alpha therapy. 19957856999
eradication of hepatitis c virus after interferon-alpha therapy. 19957857000
heterogeneity of liver-kidney microsomal autoantibodies in chronic hepatitis c and d virus infection.anti-liver-kidney microsomal (lkm) autoantibodies occur in a proportion of patients with chronic hepatitis c and d infections. because of different immunofluorescence patterns, antibodies in hepatitis c and d were termed lkm-1 and lkm-3, respectively. the aim of the present study was to evaluate the different specificities of lkm-1 and lkm-3 antibodies.19957835588
susceptibility of human t-lymphotropic virus type i infected cell line mt-2 to hepatitis c virus infection.to obtain a hepatitis c virus (hcv) proliferation system, we examined the susceptibility of various cultured cell lines to hcv infection. we found that a human t-lymphotropic virus type i infected cell line mt-2 was fairly sensitive to hcv infection. using the polymerase chain reaction, intracellular positive-stranded hcv rna was detected until at least 15 days postinoculation (p.i.). intracellular negative-stranded hcv rna was also detected at 10 days p.i., although not at 7 days p.i., suggesti ...19957832798
hepatitis c virus rna in factor viii concentrates.hepatitis c virus (hcv) rna was measured in commercial factor viii concentrates, that is, antihemophilic factor (human) (ahf), to allow the retrospective evaluation of the effect of various virus-inactivation procedures. the impact on ahf of recent anti-hcv screening of plasma was also investigated.19957825205
antibodies anti-parvovirus b19 in chronic hepatitis c virus infection. 19957717346
posttransfusional, lkm-1-autoantibody-positive hepatitis c virus infection, cryoglobulinemia, and aplastic anemia.aplastic anemia is occasionally caused by viral hepatitis, hepatitis c virus being the most important factor. pathogenetically, decreased bone marrow function, abnormalities of the bone marrow microenvironment, and immune-mediated suppression of hematopoiesis are important. hepatitis c virus infection is associated with a variety of extrahepatic manifestations including autoimmune features like cryoglobulinemia, sjögren's syndrome, and autoimmune hepatitis. here we report the case of a 42-year-o ...19957720468
modulation of the trans-suppression activity of hepatitis c virus core protein by phosphorylation.we previously demonstrated that the core protein of hepatitis c virus (hcv) can suppress gene expression and replication of hepatitis b virus (hbv) in a human hepatoma cell line (huh-7). in this study, we have characterized the phosphorylation property of hcv core protein and examined the effect of phosphorylation on its suppressive activity of hbv. our results indicated that both the full-length hcv core protein (22 kda) and its processed or degraded forms (14 to 18 kda) were phosphorylated in ...19957815494
sequence evolution of the hypervariable region in the putative envelope region e2/ns1 of hepatitis c virus is correlated with specific humoral immune responses.sequence evolution of the hypervariable region 1 (hvr1) in the n terminus of e2/ns1 of hepatitis c virus (hcv) was studied retrospectively in six chimpanzees inoculated with the same genotype 1b strain, containing a unique predominant hvr1 sequence. immediately after inoculation, all animals contained the same hvr predominant sequence. two animals developed an acute self-limiting infection. anti-hvr1 immunoglobulin g (igg) was produced 40 to 60 days after inoculation and rapidly disappeared afte ...19957815542
membranoproliferative glomerulonephritis associated with hepatitis c virus infection responsive to interferon-alpha.a 42-year-old man with hepatitis c virus infection, cryoglobulinemia, hypocomplementemia, and nephrotic syndrome is reported. the kidney biopsy showed membranoproliferative glomerulonephritis. the patient was treated with interferon-alpha for 2 months. after the treatment, serum hepatitis c virus rna became negative and nephrotic syndrome remitted. repeat biopsy of the kidney revealed an improvement in renal histology. this case confirms an association between hepatitis c virus infection and mem ...19957810536
perinatal transmission and manifestation of hepatitis c virus infection in a high risk population.we studied the perinatal transmission of hepatitis c virus (hcv) in 70 high risk mother/infant pairs. seventy-six percent of the mothers (53 of 70) were coinfected with human immunodeficiency virus (hiv) and 79% (55 of 70) had a history of drug addiction. during the follow-up hcv rna was detected in 14 of 70 (20%) infants: 12% (2 of 17) in infants born to hiv-negative mothers; and 23% (12 of 53) in infants to hiv-positive mothers. the rate of vertical transmission was significantly higher in vag ...19957761184
molecular mimicry between fc receptor and s peplomer protein of mouse hepatitis virus, bovine corona virus, and transmissible gastroenteritis virus.we have previously demonstrated molecular mimicry between the s peplomer protein of mouse hepatitis virus (mhv) and fc gamma r (fc gamma r). a monoclonal antibody (mab) to mouse fc gamma r (2.4g2 anti-fc gamma r mab), purified rabbit immunoglobulin, but not their f(ab')2 fragments, as well as mouse and rat igg, immunoprecipitated (1) recombinant s peplomer protein expressed by a vaccinia virus recombinant in human, rabbit, and mouse cells, and (2) natural s peplomer protein from cells infected w ...19957768529
hypocomplementaemic urticarial vasculitis, interstitial lung disease and hepatitis c.a 42-year-old man developed persistent urticaria and angio-oedema responsive to systemic steroids. a skin biopsy revealed leucocytoclastic vasculitis. c4 levels were consistently decreased in the setting of a mildly elevated erythrocyte sedimentation rate. pulmonary function studies revealed a restrictive pattern, and there was eosinophilia in bronchial lavage fluid. antibody to hepatitis c virus was positive, and there was mild elevation of serum aminotransferase. we believe that this case repr ...19957772493
serosurvey of human immunodeficiency virus, hepatitis b virus, and hepatitis c virus infection among hospital-based surgeons. serosurvey study group.because occupational blood contact places health-care workers at risk for infection with bloodborne pathogens, we wanted to estimate the prevalence of infection with human immunodeficiency virus (hiv), hepatitis b virus (hbv), and hepatitis c virus (hcv) among hospital-based surgeons and correlate the results with occupational and nonoccupational risk factors.19958000651
[transmission of hepatitis c viruses: certainties and hypotheses].since the identification of the hepatitis c virus in 1989, much progress has been made in understanding viral transmission. it is now known that the major route of contamination occurs via parenteral transmission (blood transfusion, injections, intravenous drug abuse, haemodialysis...). we are however less certain about sexual, mother-child, and person to person transmission. several cofactors have been suggested as influencing transmission including the stage of the disease, coinfection with th ...19957899341
fetal tissue derived from spontaneous pregnancy losses is insufficient for human transplantation.to determine if sufficient fetal tissue with desirable transplant characteristics can be obtained from spontaneous abortions.19957898844
intrahepatic expression of hepatitis c virus antigens in chronic liver disease.localization of hepatitis c virus (hcv) antigens was studied in fresh frozen and formalin-fixed, paraffin-embedded liver tissue by immunoperoxidase using monoclonal antibodies to nucleocapsid protein and polyclonal human immunoglobulin g purified from plasma containing antibodies to structural and non-structural antigens of hepatitis c virus. the results observed using monoclonal antibody to hcv core were similar to those of polyclonal igg against hcv antigens in the majority of cases and both c ...19957891230
the risk of human t cell leukemia virus and viral hepatitis infection among us marines stationed in okinawa, japan.the prevalence and incidence of human t cell leukemia virus type i/ii (htlv-i/ii) and hepatitis a, b, and c virus infection were determined among us marines stationed in okinawa, japan. of 2875 personnel, 2 (0.07%) had antibody to htlv-i/ii. after 1-3 years, no htlv seroconversions were observed, although 23% reported sexual contact with okinawans. of 1010 hepatitis-tested marines, 121 (12%) had antibody to hepatitis a virus (anti-hav), 26 (2.6%) had antibody to hepatitis b core antigen (anti-hb ...19957876620
transfection of a differentiated human hepatoma cell line (huh7) with in vitro-transcribed hepatitis c virus (hcv) rna and establishment of a long-term culture persistently infected with hcv.t7 rna polymerase transcripts of a putative full-length cdna clone of hepatitis c virus type 1 (hcv-1) were used to transfect a differentiated human hepatoma cell line, huh7. the transfected genome replicated in cells, as evidenced by the appearance of progeny hcv rna, detection of negative-strand viral rna, and incorporation of [3h]uridine into the viral genome. incubation of naive huh7 cells with conditioned medium from transfected cells resulted in a new hcv infection, suggesting the producti ...19957983724
overview: viral agents and cancer.substantial evidence indicates that several common viruses are clearly or probable causal factors in the etiology of specific malignancies. these viruses either normally establish latency or can become persistent infections. oncogenesis is probably linked to an enhanced level of viral activation in the infected host, reflecting heavy viral dose or compromised immune control. the major virus-malignancy systems include hepatitis b virus (hbv), hepatitis c virus (hcv), and hepatocellular carcinoma; ...19958741795
the gb hepatitis viruses.the genomes of three new flavi-like viruses, gbv-a, gbv-b and gbv-c have been identified. nucleic acid molecules corresponding to the genomes of gbv-a and gbv-b were isolated from tamarins with hepatitis which had been infected with the gb agent. rna sequences corresponding to gbv-c have been shown to be present in sera from humans with non-a-e hepatitis. sequence comparisons show that these three viruses are more closely related to each other and to hepatitis c virus (hcv) than to any other kno ...19958745313
identification of hepatitis c virus by immunoelectron microscopy.sequencing of the hepatitis c virus (hcv) has provided a better understanding of the natural history, immunology, and epidemiology of this virus. however, the morphology of hcv has not been definitively characterized. in this study, through a sequence of concentration processes, virus-like particles were isolated from human serum and liver tissue, visualized by transmission electron microscopy and identified as hepatitis c virion by immunoelectron microscopy. spherical flavi-like virus particles ...19958745314
revealing the history of infectious disease epidemics through phylogenetic trees.phylogenetic trees play an increasing role in molecular epidemiology, where they have been used to understand the forces that shape patterns of viral sequence diversity. phylogenetic trees can also be used to trace the dynamics of viral transmission within populations. case studies document the worldwide spread of human immunodeficiency virus type 1 (hiv-1) and hepatitis c virus (hcv). despite similarities between these viruses, especially in their transmission routes, they are shown to have ver ...19958748017
[avellis syndrome in systemic rheumatoid vasculitis].a 74-year-old man presented sudden onset hoarseness and dysphagia. two months before this event, he had developed arthralgia of the shoulders, elbows, hands and foot and pleuritis which had been alleviated by a treatment with prednisolone. on admission, the patient could not phonate nor swallow at all. his soft palate was elevated at the right side. the uvula moved left when the patient tried to speak. laryngoscopic examination revealed the paralysis of right vocal cord. the erythrocyte sediment ...19958821503
characterization of human t cell clones specific for coronavirus 229e.coronaviruses (cv) are pleomorphic enveloped rna viruses that are ubiquitous in nature, causing a variety of diseases in both man and domestic animals. in man, cv are generally associated with upper respiratory tract infections. the two prototype strains that are the best studied human cv isolates and which are thought to be responsible for most of the respiratory infections caused by cv are called 229e and oc43. humoral responses consisting of neutralizing antibodies to cv are present in most i ...19958830466
multiple receptor-dependent steps determine the species specificity of hcv-229e infection.human coronavirus (hcv)-229e causes disease only in humans and grows in human cells and in cells of other species that express recombinant human aminopeptidase n (hapn), the receptor for hcv-229e. we compared the species specificity of hcv-229e infection with the species specificity of virus binding using immunofluorescence, assay of virus yields, fluorescence activated cell sorting and a monoclonal antibody directed against hapn that blocks infection. we found that hcv-229e binds to intestinal ...19958830504
analysis of cellular receptors for human coronavirus oc43.bovine coronavirus (bcv), human coronavirus oc43 (hcv-oc43) and hemagglutinating encephalomyelitis virus (hev) are serologically related viruses that all have hemagglutinating activity. the receptor determinant for attachment to erythrocytes has been shown to be n-acetyl-9-o-acetylneuraminic acid (neu5,9ac2). we compared the ability of the three coronaviruses to recognize 9-o-acetylated sialic acid and found that they all bind to neu5,9ac2 attached to galactose in either a2,3 or a2,6-linkage. th ...19958830510
prevalence of blood-borne viruses among intravenous drug users and alcoholics in hiroshima, japan.we investigated the prevalence of human immunodeficiency viruses-1 and 2 (hiv-1 and hiv-2), human t-lymphotropic virus type i and ii, hepatitis b virus (hbv), hepatitis c virus (hcv) and hepatitis d virus among intravenous drug users (ivdu) in hiroshima, japan, where little is known about their present levels. from june to december 1993, serum samples were collected from 47 ivdu and 98 alcoholics in hiroshima, japan, and examined for markers of virus infection. the prevalence of antibody to hcv ...19958845404
a minimal and optimal cytotoxic t cell epitope within hepatitis c virus nucleoprotein.amino acid residues 81-100 of the hepatitis c virus (hcv) nucleoprotein contain a cytotoxic t cell epitope that is recognized by cytotoxic t lymphocytes (ctls) in association with human leukocyte antigen b44. with panels of truncated and overlapping peptides, the minimal and optimal epitope recognized by ctls was shown to be a 9-mer peptide (residues 88-96). the peptide can stimulate effectively ctls that are able to recognize endogenously synthesized and processed hcv nucleoprotein.19958847528
sexually transmitted diseases in australia: a decade of change. epidemiology and surveillance.survival data in the last decade for sexually transmitted diseases (stds) other than human immunodeficiency virus (hiv) are uneven across the states/territories. the incidence of gonorrhoea decreased by more than 80%, but at different times in different patient groups, different states, and different anatomical sites. there was a resurgence of rectal gonorrhoea in homosexual men in 1989-1991. resistance to penicillin steadily increased, and partial resistance to quinolones has emerged. there was ...19958849191
[prevalence of human herpesvirus 7 in mexican blood donors].the human herpes virus 7 (hhv-7) has been recently isolated from cd4 cells of healthy persons. the present study describes the antibody prevalence of this virus in a healthy mexican population. two hundred blood samples from candidates for blood donation at the hospital general de mexico were studied with the indirect immunofluorescence test (ifa) in hhv-7 infected supt1 cells. the testing was done in the university of cologne, germany; 167 were males and 33 female; the donors came from 12 of th ...19958850145
Refresh
firstprev
12345678910...
nextlast
Displaying items 801 - 900 of 8967