Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [current topics and risk management of listeria monocytogenes in food]. | 2005 | 16180692 | |
| p-ser-hpr--a link between carbon metabolism and the virulence of some pathogenic bacteria. | hpr kinase/phosphorylase phosphorylates hpr, a phosphocarrier protein of the phosphoenolpyruvate:carbohydrate phosphotransferase system, at serine-46. p-ser-hpr is the central regulator of carbon metabolism in gram-positive bacteria, but also plays a role in virulence development of certain pathogens. in listeria monocytogenes, several virulence genes, which depend on the transcription activator prfa, are repressed by glucose, fructose, etc., in a catabolite repressor (ccpa)-independent mechanis ... | 2005 | 16182622 |
| arhgap10 is necessary for alpha-catenin recruitment at adherens junctions and for listeria invasion. | e-cadherin mediates the formation of adherens junctions between epithelial cells. it serves as a receptor for listeria monocytogenes, a bacterial pathogen that enters epithelial cells. the l. monocytogenes surface protein, inla, interacts with the extracellular domain of e-cadherin. in adherens junctions, this ectodomain is involved in homophilic interactions whereas the cytoplasmic domain binds beta-catenin, which then recruits alpha-catenin. alpha-catenin binds to actin directly, or indirectly ... | 2005 | 16184169 |
| identification and characterization of di- and tripeptide transporter dtpt of listeria monocytogenes egd-e. | listeria monocytogenes is a gram-positive intracellular pathogen responsible for opportunistic infections in humans and animals. here we identified and characterized the dtpt gene (lmo0555) of l. monocytogenes egd-e, encoding the di- and tripeptide transporter, and assessed its role in growth under various environmental conditions as well as in the virulence of l. monocytogenes. uptake of the dipeptide pro-[14c]ala was mediated by the dtpt transporter and was abrogated in a deltadtpt isogenic de ... | 2005 | 16204487 |
| long-term survival of pathogenic and sanitation indicator bacteria in experimental biowaste composts. | for economic, agricultural, and environmental reasons, composting is frequently used for organic waste recycling. one approach to limiting the potential risk from bacterial food-borne illnesses is to ensure that soil amendments and organic fertilizers are disinfected. however, more knowledge concerning the microbiological safety of composted substrates other than sludge and manure is necessary. experimental in-vessel biowaste composts were used to study the survival of seeded listeria monocytoge ... | 2005 | 16204488 |
| microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in lactobacillus acidophilus. | two-component regulatory systems are one primary mechanism for environmental sensing and signal transduction. annotation of the complete genome sequence of the probiotic bacterium lactobacillus acidophilus ncfm revealed nine two-component regulatory systems. in this study, the histidine protein kinase of a two-component regulatory system (lba1524hpk-lba1525rr), similar to the acid-related system lisrk from listeria monocytogenes (p. d. cotter et al., j. bacteriol. 181:6840-6843, 1999), was inser ... | 2005 | 16204490 |
| genetic diversity of listeria monocytogenes strains from a high-prevalence dairy farm. | listeria monocytogenes is a significant food-borne human and veterinary pathogen. contaminated silage commonly leads to disease in livestock, but the pervasive nature of the bacterium can make it difficult to identify the source of infection. an investigation of bovine listeriosis that occurred on a pacific northwest dairy farm ("farm a") revealed that the clinical strain was closely related to fecal strains from asymptomatic cows, and that farm environment was heavily contaminated with a divers ... | 2005 | 16204502 |
| investigation of specific substitutions in virulence genes characterizing phenotypic groups of low-virulence field strains of listeria monocytogenes. | several models have shown that virulence varies from one strain of listeria monocytogenes to another, but little is known about the cause of low virulence. twenty-six field l. monocytogenes strains were shown to be of low virulence in a plaque-forming assay and in a subcutaneous inoculation test in mice. using the results of cell infection assays and phospholipase activities, the low-virulence strains were assigned to one of four groups by cluster analysis and then virulence-related genes were s ... | 2005 | 16204519 |
| rsa 2004: combined basic research satellite symposium-mechanisms of alcohol-mediated organ and tissue damage: inflammation and immunity and alcohol and mitochondrial metabolism: at the crossroads of life and death session one: alcohol, cellular and organ damage. | this article summarizes content proceedings of a satellite meeting held at the 2004 research society on alcoholism annual scientific meeting in vancouver, canada. the aim of the satellite conference was to facilitate the interaction of scientists investigating the mechanisms of alcohol-mediated organ or tissue damage, and enable the discussion and sharing of new ideas and concepts that may be common in each of the organs or tissues affected by chronic ethanol consumption. the original planned pr ... | 2005 | 16205374 |
| sigmab contributes to listeria monocytogenes invasion by controlling expression of inla and inlb. | the ability of listeria monocytogenes to invade non-phagocytic cells is important for development of a systemic listeriosis infection. the authors previously reported that a l. monocytogenes delta sigb strain is defective in invasion into human intestinal epithelial cells, in part, due to decreased expression of a major invasion gene, inla. to characterize additional invasion mechanisms under the control of sigmab, mutants were generated carrying combinations of in-frame deletions in inla, inlb ... | 2005 | 16207905 |
| multi-virulence-locus sequence typing clarifies epidemiology of recent listeriosis outbreaks in the united states. | multi-virulence-locus sequence typing (mvlst) was used to analyze isolates from two major listeriosis outbreaks in the united states in 1998 and 2002 that were due to consumption of contaminated hot dogs and turkey deli meat, respectively. mvlst demonstrated high epidemiological relevance and indicated that the two outbreaks were the result of one epidemic. | 2005 | 16208000 |
| paradoxical anti-inflammatory actions of tnf-alpha: inhibition of il-12 and il-23 via tnf receptor 1 in macrophages and dendritic cells. | il-12 and tnf-alpha are central proinflammatory cytokines produced by macrophages and dendritic cells. disregulation of tnf-alpha is associated with sepsis and autoimmune diseases such as rheumatoid arthritis. however, new evidence suggests an anti-inflammatory role for tnf-alpha. tnf-alpha-treated murine macrophages produced less il-12p70 and il-23, after stimulation with ifn-gamma and lps. frequency of il-12p40-producing macrophages correspondingly decreased as measured by intracellular cytoki ... | 2005 | 16210605 |
| attenuation of th1 response in decoy receptor 3 transgenic mice. | the soluble decoy receptor 3 (dcr3) is a member of the tnfr superfamily. because dcr3 is up-regulated in tumor tissues and is detectable in the sera of cancer patients, it is regarded as an immunosuppressor to down-regulate immune responses. to understand the function of dcr3 in vivo, we generated transgenic mice overexpressing dcr3 systemically. in comparison with hnt-tcr (hnt) transgenic mice, up-regulation of il-4 and il-10 and down-regulation of ifn-gamma, il-12, and tnf-alpha were observed ... | 2005 | 16210617 |
| purification of bovine milk lactoperoxidase and investigation of antibacterial properties at different thiocyanate mediated. | bovine lactoperoxidase (lpo) was purified with amberlite cg 50 h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography from skim milk. the activity of lactoperoxidase was measured by using 2.2-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (abts) as a choromogenic substrate at ph 6.0. purification degree for the purified enzyme was controlled with sds-page and rz value (a412/a280). rz value for the purified lpo was 0.8. km value ... | 2005 | 16212035 |
| response regulator degu of listeria monocytogenes regulates the expression of flagella-specific genes. | an isogenic mutant of listeria monocytogenes egd with a deletion of the response regulator gene degu showed a lack of motility due to the absence of flagella. in the present study, we used two-dimensional gel electrophoresis, mass-spectrometry and microarray analyses to identify the listerial genes that depend on degu for expression. we found that the two l. monocytogenes operons encoding flagella-specific genes and the monocistronically transcribed flaa gene are positively regulated by degu at ... | 2005 | 16213668 |
| correlation of probiotic lactobacillus salivarius growth phase with its cell wall-associated proteome. | lactobacillus salivarius subsp. salivarius ucc118 is a probiotic bacterium that was originally isolated from human intestinal tissues and was subsequently shown in a pilot study to alleviate symptoms associated with mild-moderate crohn's disease. strain ucc118 can adhere to animal and human intestinal tissue, and to both healthy and inflamed ulcerative colitis mucosa, irrespective of location in the gut. in this study, an enzymatic technique has been combined with proteomic analysis to correlate ... | 2005 | 16214296 |
| role of flif and flii of listeria monocytogenes in flagellar assembly and pathogenicity. | flagellar structures have been shown to participate in virulence in a variety of intestinal pathogens. here, we have identified two potential flagellar genes of listeria monocytogenes: lmo0713, encoding a protein similar to the flagellar basal body component flif, and lmo0716, encoding a protein similar to flii, the cognate atpase energizing the flagellar export apparatus. expression of flif and flii appears to be downregulated at 37 degrees c, like that of flaa, encoding flagellin. by construct ... | 2005 | 16113269 |
| pathogenicity and immunogenicity of a vaccine strain of listeria monocytogenes that relies on a suicide plasmid to supply an essential gene product. | listeria monocytogenes is a bacterial pathogen that elicits a strong cellular immune response and thus has potential use as a vaccine vector. an attenuated strain, l. monocytogenes dal dat, produced by deletion of two genes (dal and dat) used for d-alanine synthesis, induces cytotoxic t lymphocytes and protective immunity in mice following infection in the presence of d-alanine. in order to obviate the dependence of l. monocytogenes dal dat on supplemental d-alanine yet retain its attenuation an ... | 2005 | 16113297 |
| sex-dependent susceptibility to listeria monocytogenes infection is mediated by differential interleukin-10 production. | it is well documented that sex-dependent factors affect susceptibility to infection, with most mouse models demonstrating higher resistance in females. we made the unexpected observation that infection with the intracellular bacterium listeria monocytogenes showed an opposite pattern in several commonly used inbred mouse strains: female c57bl/6j, balb/c, c3h/hen, and cba/j mice were significantly more susceptible to listeria infection. the pronounced sensitivity of females to listeria, which was ... | 2005 | 16113316 |
| the mycobacterium tuberculosis esat-6 homologue in listeria monocytogenes is dispensable for growth in vitro and in vivo. | esat-6 is a virulence determinant in mycobacterium tuberculosis and a member of a conserved group of proteins in a variety of other bacteria. a targeted deletion of the homologous gene in listeria was generated, and in contrast to that observed for mycobacteria, this locus was not required for listeria virulence. | 2005 | 16113338 |
| involvement of reactive oxygen intermediate in the enhanced expression of virulence-associated genes of listeria monocytogenes inside activated macrophages. | listeriolysin o encoded by 1,587 bp hly is the essential virulence factor of listeria monocytogenes that replicates in the cytosolic space after escaping from phagosome of macrophages. by using murine macrophage-like j774.1 cells with or without activation by ifn-gamma plus lps, the expression of both hly and its positive regulator prfa was monitored by means of rt-pcr. in activated j774.1 cells, the level of hly expression was enhanced although the multiplication of bacteria was significantly s ... | 2005 | 16113511 |
| listeria hijacks the clathrin-dependent endocytic machinery to invade mammalian cells. | the bacterial pathogen listeria monocytogenes uses the surface protein inlb to invade a variety of cell types. the interaction of inlb with the hepatocyte growth-factor receptor, met, is crucial for infection to occur. remarkably, the ubiquitin ligase cbl is rapidly recruited to inlb-activated met. recent studies have shown that ligand-dependent endocytosis of met and other receptor tyrosine kinases is triggered by monoubiquitination of the receptor, a process that is mediated by cbl. here, we s ... | 2005 | 16113677 |
| a study on inhibitory effects of siğla tree (liquidambar orientalis mill. var. orientalis) storax against several bacteria. | in this study, siğla (liquidambar orientalis mill.) storax (styrax) was investigated for antibacterial activity against aeromonas hydrophila, bacillus amyloliquefaciens, b. brevis, b. cereus, b. megaterium, b. subtilis, corynebacterium xerosis, enterobacter aerogenes, enterococcus faecalis, escherichia coli, e. coli o157:h7, klebsiella pneumoniae, listeria monocytogenes, micrococcus luteus, mycobacterium smegmatis, proteus vulgaris, pseudomonas aeruginosa, p. fluorescens, staphylococcus aureus a ... | 2005 | 16114094 |
| intracellular bacteria differentially regulated endothelial cytokine release by mapk-dependent histone modification. | epigenetic histone modifications contribute to the regulation of eukaryotic gene transcription. the role of epigenetic regulation in immunity to intracellular pathogens is poorly understood. we tested the hypothesis that epigenetic histone modifications influence cytokine expression by intracellular bacteria. intracellular listeria monocytogenes, but not noninvasive listeria innocua, induced release of distinct cc and cxc chemokines, as well as th1 and th2 cytokines and growth factors by endothe ... | 2005 | 16116170 |
| in vivo generation of pathogen-specific th1 cells in the absence of the ifn-gamma receptor. | the precise mechanisms that govern the commitment of cd4 t cells to become th1 or th2 cells in vivo are incompletely understood. recent experiments demonstrate colocalization of the ifn-gammar chains with the tcr during activation of naive cd4 t cells, suggesting that association of these molecules may be involved in determining lineage commitment. to test the role of ifn-gamma and its receptor in the generation of th1 ag-specific cd4 t cells, we analyzed mice after infection with listeria monoc ... | 2005 | 16116201 |
| listeria monocytogenes phosphatidylinositol-specific phospholipase c has evolved for virulence by greatly reduced activity on gpi anchors. | listeria monocytogenes phosphatidylinositol-specific phospholipase c (pi-plc) plays a critical role in escape of this human pathogen from host cell vacuoles. unlike classical bacterial pi-plcs, the l. monocytogenes enzyme has very weak activity on glycosylphosphatidylinositol (gpi)-anchored proteins. previous crystal structure analysis has revealed that a small beta-strand (vb) is present in bacillus cereus pi-plc and is absent in the enzyme from l. monocytogenes. this vb beta-strand in b. cereu ... | 2005 | 16118276 |
| behavior of listeria monocytogenes in ph-modified chicken salad during refrigerated storage. | the growth and inactivation kinetics of l. monocytogenes were evaluated at ph 4.0, 4.6, and 5.2 during storage at 5.0 degrees c, 7.2 degrees c, and 21.1 degrees c (41 degrees f, 45 degrees f, and 70 degrees f). using commercially produced pasteurized chicken salad, the authors adjusted the ph levels with acetic acid or sodium acetate. samples of 25 g each of the ph-modified salad were inoculated to approximately 1 x 10(6) cells per gram with a three-strain mixture of l. monocytogenes and stored ... | 2005 | 16121485 |
| unusual inflammatory and fibrogenic pulmonary responses to single-walled carbon nanotubes in mice. | single-walled carbon nanotubes (swcnt) are new materials of emerging technological importance. as swcnt are introduced into the life cycle of commercial products, their effects on human health and environment should be addressed. we demonstrated that pharyngeal aspiration of swcnt elicited unusual pulmonary effects in c57bl/6 mice that combined a robust but acute inflammation with early onset yet progressive fibrosis and granulomas. a dose-dependent increase in the protein, ldh, and gamma-glutam ... | 2005 | 15951334 |
| accelerated cd8+ t-cell memory and prime-boost response after dendritic-cell vaccination. | efficient boosting of memory t-cell numbers to protective levels generally requires a relatively long interval between immunizations. decreasing this interval could be crucial in biodefense and cancer immunotherapy, in which rapid protective responses are essential. here, we show that vaccination with peptide-coated dendritic cells (dcs) generated cd8+ t cells with the phenotype and function of memory cells within 4-6 d. these early memory cd8+ t cells underwent vigorous secondary expansion in r ... | 2005 | 15951824 |
| the increase in mucin exocytosis and the upregulation of muc genes encoding for membrane-bound mucins induced by the thiol-activated exotoxin listeriolysin o is a host cell defence response that inhibits the cell-entry of listeria monocytogenes. | in vivo listeria monocytogenes infection results in the massive release of mucus by goblet cells into the lumen of the intestine. we have previously reported that apical infection by l. monocytogenes is followed by listeriolysin o (llo)-dependent stimulation of mucus exocytosis, and the upregulation of the muc genes. here, we report that l. monocytogenes egd wild-type bacteria enter cultured human polarized, mucin-secreting, ht29-mtx cells apically by an inla-dependent mechanism. the llo-induced ... | 2005 | 15953034 |
| comparison of the microflora on organically and conventionally grown spring mix from a california processor. | considerable speculation has occurred concerning the potential for higher numbers of foodborne pathogens on organically grown produce compared with produce not grown organically. the microflora composition of spring mix or mesclun, a mixture of multiple salad ingredients, grown either by organic or conventional means was determined. unwashed or washed spring mix was obtained from a commercial california fresh-cut produce processor who does not use manure in their cultivation practices. fifty-fou ... | 2005 | 15954699 |
| influence of dietary vitamin e on behavior of listeria monocytogenes and color stability in ground turkey meat following electron beam irradiation. | there is growing concern that the free radical scavenging effect of antioxidants added to meats might reduce the antimicrobial effectiveness of ionizing radiation. a study was conducted to determine the effect of vitamin e on the behavior (growth) of listeria monocytogenes and color stability in turkey meat following electron beam irradiation. raw ground turkey breast meat from birds fed diets containing 0 (control), 50, 100, and 200 iu/kg of vitamin e was inoculated with a five-strain mixture o ... | 2005 | 15954702 |
| evaluation of gaseous chlorine dioxide as a sanitizer for killing salmonella, escherichia coli o157:h7, listeria monocytogenes, and yeasts and molds on fresh and fresh-cut produce. | gaseous chlorine dioxide (clo2) was evaluated for effectiveness in killing salmonella, escherichia coli o157:h7, and listeria monocytogenes on fresh-cut lettuce, cabbage, and carrot and salmonella, yeasts, and molds on apples, peaches. tomatoes, and onions. inoculum (100 microl, ca. 6.8 log cfu) containing five serotypes of salmonella enterica, five strains of e. coli o157:h7, or five strains of l. monocytogenes was deposited on the skin and cut surfaces of fresh-cut vegetables, dried for 30 min ... | 2005 | 15954704 |
| raw and processed fish show identical listeria monocytogenes genotypes with pulsed-field gel electrophoresis. | a total of 257 raw fish samples at two different sites were examined for the presence of listeria monocytogenes. the prevalence of l. monocytogenes was 4%. from 11 positive samples, nine different l. monocytogenes pulsed-field gel electrophoresis genotypes were recovered. from nine pulsotypes recovered from raw fish and 32 pulsotypes shown by 101 fish product isolates, two raw fish and fish product pulsotypes were indistinguishable from each other. although the prevalence of l. monocytogenes in ... | 2005 | 15954713 |
| avirulence of viable but non-culturable listeria monocytogenes cells demonstrated by in vitro and in vivo models. | the virulence of viable but non-culturable (vbnc) cells of 4 strains of listeria monocytogenes was investigated in both a human adenocarcinoma cell line (ht-29) and a mouse model. lo 28, atcc 19115 and cnl 895807 strains of listeria monocytogenes became vbnc when incubated in microcosm water at 20 degrees c and scott a strain at 4 degrees c. no culturable bacteria were detected in the vbnc state, although 104 active cells/ml were found by the direct viable count (dvc) and ctc-dapi double stainin ... | 2005 | 15955283 |
| declines of zoonotic agents in liquid livestock wastes stored in batches on-farm. | to measure the decline rates of zoonotic agents introduced into liquid livestock wastes in on-farm storage tanks. | 2005 | 15960665 |
| shelf life and safety aspects of chilled cooked and peeled shrimps (pandalus borealis) in modified atmosphere packaging. | to evaluate the growth of listeria monocytogenes and shelf life of cooked and peeled shrimps in modified atmosphere packaging (map). | 2005 | 15960666 |
| occurrence of sublethal injury after pulsed electric fields depending on the micro-organism, the treatment medium ph and the intensity of the treatment investigated. | the objective was to investigate the occurrence of sublethal injury after pulsed electric field (pef) depending on the treatment time, the electric field strength and the ph of the treatment media in two gram-positive (bacillus subtilis ssp. niger, listeria monocytogenes) and six gram-negative (escherichia coli, escherichia coli o157:h7, pseudomonas aeruginosa, salmonella serotype senftenberg 775w, salmonella serotype typhimurium, yersinia enterocolitica) bacterial strains. | 2005 | 15960669 |
| an extract from teak (tectona grandis) bark inhibited listeria monocytogenes and methicillin resistant staphylococcus aureus. | the aim of this study was to characterize the inhibitory mechanism in teak (tectona grandis) bark and to determine its effectiveness against listeria monocytogenes and methicillin resistant staphylococcus aureus (mrsa). | 2005 | 15960759 |
| "lanespector", a tool for membrane proteome profiling based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis/liquid chromatography-tandem mass spectrometry analysis: application to listeria monocytogenes membrane proteins. | proteomics is required to provide insight into any type of subproteome. while the workflow based on two-dimensional polyacrylamide gel electrophoresis (2-d page) can be applied for many subproteomes and comprises well-established strategies for data presentation and data analysis, the comprehensive investigation of membrane proteomes remains a challenging task. we present a number of procedures that provide an insight into such systems. we have established a novel protocol for the efficient prep ... | 2005 | 15966022 |
| in vitro activity of daptomycin against clinical isolates of gram-positive bacteria. | we determined the activity of daptomycin, a recently fda-approved antimicrobial agent, against clinical isolates of gram-positive bacteria, including viridans group streptococci (16 streptococcus mitis species group, 12 s. mutans species group, 9 s. anginosus species group, 8 s. sanguinis species group, 5 s. salivarius species group) from patients with infective endocarditis, 32 methicillin-resistant staphylococcus aureus, 32 high-level penicillin-resistant streptococcus pneumoniae, 38 vancomyci ... | 2005 | 16133715 |
| no indication for a defect in toll-like receptor signaling in patients with hyper-ige syndrome. | hyper-ige syndrome is a rare primary immunodeficiency of unknown etiology characterized by recurrent infections of the skin and respiratory system, chronic eczema, elevated total serum ige, and a variety of associated skeletal symptoms. recent reports about susceptibility to pyogenic bacterial infections and high ige levels in patients and animals with defects in toll-like receptor (tlr) signaling pathways prompted us to search for tlr signaling defects as an underlying cause of hyper-ige syndro ... | 2005 | 16133988 |
| [listeria monocytogenes in women of reproductive age]. | listeria monocytogenes (lm) is gram-positive bacteria linear shaped which grows good also at refrigerator temperature (4 degrees c). the bacteria is resistant on high and low temperatures. this bacteria can be found in the dirt, rotted vegetation, vegetables and fruit, milk and dairy products, and also in the meat and processed meat. this bacteria cause disease called listeriosis. this bacteria attacks at first people with weak immune system like infants, pregnant women, people with chronic dise ... | 2005 | 16134751 |
| semiparametric methods for mapping quantitative trait loci with censored data. | statistical methods for the detection of genes influencing quantitative traits with the aid of genetic markers are well developed for normally distributed, fully observed phenotypes. many experiments are concerned with failure-time phenotypes, which have skewed distributions and which are usually subject to censoring because of random loss to follow-up, failures from competing causes, or limited duration of the experiment. in this article, we develop semiparametric statistical methods for mappin ... | 2005 | 16135030 |
| the adjuvant activity of cpg dna requires t-bet expression in dendritic cells. | treatment with synthetic oligodeoxynucleotides containing cpg motifs (cpg odns) is remarkably protective against otherwise lethal infection. here, we describe an essential role for the transcription factor t-bet in mediating the protective function of cpg odns. loss of t-bet in conventional cd11c(hi) dendritic cells (dcs) and in plasmacytoid dcs impaired production of ifns. strikingly, in contrast to rag2-/- mice, rag2-/- mice that also lacked t-bet (dko) could not be rescued from lethal listeri ... | 2005 | 16135562 |
| decrease in serum procalcitonin levels over time during treatment of acute bacterial meningitis. | the aim of this study was to describe the change in serum procalcitonin levels during treatment for community-acquired acute bacterial meningitis. | 2005 | 16137346 |
| toll-like receptor-2 deficiency is associated with enhanced brain tnf gene expression during pneumococcal meningitis. | tnf is a marker of disease activity in bacterial meningitis. to investigate tnf modulation by toll-like receptor-2 (tlr2), we studied temporal and anatomical expression patterns of tlr2 and tnf in a pneumococcal meningitis model in wild type (wt) and tlr2(-/-) mice. we show by in situ hybridization that transcripts of tlr2 and of the comolecules cd14, md-2, tlr1/6 strongly increased and colocalized with tnf in cd45-positive infiltrating cells in the ventricles, corpus callosum and the meninges. ... | 2005 | 16137770 |
| listeria monocytogenes egd lacking penicillin-binding protein 5 (pbp5) produces a thicker cell wall. | we report on the cloning of the structural gene for penicillin-binding protein 5 (pbp5), lmo2754. we also describe the enzymatic activity of pbp5 and characterize a mutant lacking this activity. purified pbp5 has dd-carboxypeptidase activity, removing the terminal d-alanine residue from murein pentapeptide side chains. it shows higher activity against low molecular weight monomeric pentapeptide substrates compared to dimeric pentapeptide compound. similarly, pbp5 preferentially cleaves monomeric ... | 2005 | 16140473 |
| [pulse-electrotypes of listeria monocytogenes strains, isolated in moscow]. | the characterization of the pulse-electrotypes of l. monocytogenes, isolated in 2003-2004 in moscow from different sources, is presented. among the cultures, isolated from humans, one outbreak pulse electrotype was detected and from different objects in buildings where a wide variety of food products was produced several probably related and unrelated pulse-electrotypes were obtained. the conclusion was made that several independent l. monocytogenes clones existed on the territory of moscow, and ... | 2005 | 16146221 |
| the ikappab protein bcl-3 negatively regulates transcription of the il-10 gene in macrophages. | nf-kappab/rel transcription factors, implicated in inflammatory and immune responses against pathogens, are regulated by ikappab proteins. the physiological and molecular function of the ikappab family member bcl-3 is understood only poorly. in this study, the role of bcl-3 in an innate immune response was examined by gene targeting. we demonstrate that bcl-3(-/-) mice are highly susceptible to listeria monocytogenes infection. this correlates with diminished production of il-12 p70 and ifn-gamm ... | 2005 | 16148099 |
| fusion to listeriolysin o and delivery by listeria monocytogenes enhances the immunogenicity of her-2/neu and reveals subdominant epitopes in the fvb/n mouse. | five overlapping fragments of rat her-2/neu have been expressed in recombinant listeria monocytogenes. each fragment of her-2/neu is secreted as a fusion protein with a truncated, nonhemolytic form of listeriolysin o (llo). lm-llo-ec1, lm-llo-ec2, and lm-llo-ec3 overlap the extracellular domain of her-2/neu, whereas lm-llo-ic1 and lm-llo-ic2 span the intracellular domain. all five strains controlled the growth of established nt-2 tumors, a rat her-2/neu-expressing tumor line derived from a spont ... | 2005 | 16148111 |
| assessment of interleukin-12, gamma interferon, and tumor necrosis factor alpha secretion in sera from mice fed with dietary lipids during different stages of listeria monocytogenes infection. | recent experimental observations have determined that long-chain n-3 polyunsaturated fatty acids suppress immune functions and are involved in the reduction of infectious disease resistance. balb/c mice were fed for 4 weeks with one of four diets containing either olive oil (oo), fish oil (fo), hydrogenated coconut oil, or a low fat level. interleukin-12p70 (il-12p70), gamma interferon (ifn-gamma), and tumor necrosis factor alpha (tnf-alpha) production in the sera of mice fed these diets and cha ... | 2005 | 16148177 |
| [pathophysiology and epidemiology of listeriosis]. | listeriosis is a severe infectious disease occurring both in humans and in most other animal species. it is caused by a gram-positive bacterium, listeria monocytogenes. this facultative intracellular pathogen is widespread in nature and infects humans through contaminated food. after ingestion, bacteria can cross the gut mucosa and disseminate via the bloodstream. they may then invade the central nervous system and the placenta, causing severe meningoencephalitis and fetoplacental infections, re ... | 2005 | 16149214 |
| omega-3 polyunsaturated fatty acid impairment of early host resistance against listeria monocytogenes infection is independent of neutrophil infiltration and function. | the primary objective of this study was to determine whether the n-3 pufa-mediated changes in host response to a listeria monocytogenes infection (e.g., cytokine production and bacterial clearance) were dependent upon neutrophils. balb/c mice were fed one of two semi-purified diets that contained either 0 or 41 g of n-3 pufa/kg. after 4 week, mice were injected with a neutrophil-depleting (rb6-8c5) or isotype-control antibody 24 h prior to infection. bacterial clearance from the liver and spleen ... | 2005 | 16150430 |
| membrane damage and microbial inactivation by chlorine in the absence and presence of a chlorine-demanding substrate. | the relationship between cell inactivation and membrane damage was studied in two gram-positive organisms, listeria monocytogenes and bacillus subtilis, and two gram-negative organisms, yersinia enterocolitica and escherichia coli, exposed to chlorine in the absence and presence of 150 ppm of organic matter (trypticase soy broth). l. monocytogenes and b. subtilis were more resistant to chlorine in distilled water. the addition of small amounts of organic matter to the chlorination medium drastic ... | 2005 | 16151082 |
| identification of genes induced in listeria monocytogenes during growth and attachment to cut cabbage, using differential display. | the food-borne pathogen listeria monocytogenes is a ubiquitous soil bacterium with the potential to contaminate fresh produce during cultivation and postharvest processing. in order to identify potential mechanisms by which l. monocytogenes may successfully attach to and colonize fresh produce, gene expression in l. monocytogenes cells inoculated onto fresh-cut cabbage was compared to gene expression in cells grown under control conditions. differential display of reverse transcriptase pcr fragm ... | 2005 | 16151109 |
| surface attachment of listeria monocytogenes is induced by sublethal concentrations of alcohol at low temperatures. | sublethal concentrations of ethanol or isopropanol increased attachment of listeria monocytogenes at 10, 20, or 30 degrees c; no induction occurred at 37 degrees c. the alcohol induction phenotype was retained in sigb and cesrk mutants; however, the degree of induction was affected. these results suggest that alcohol may contribute to the persistence of l. monocytogenes. | 2005 | 16151157 |
| secretion of recombinant pediocin pa-1 by bifidobacterium longum, using the signal sequence for bifidobacterial alpha-amylase. | a recombinant dna, encoding the chimeric protein of the signal sequence for bifidobacterial alpha-amylase mature pediocin pa-1, was introduced into bifidobacterium longum mg1. biologically active pediocin pa-1 was successfully secreted from the strain and showed bactericidal activity against listeria monocytogenes and the same molecular mass as native pediocin pa-1. | 2005 | 16151166 |
| molecular characterization of listeria monocytogenes of the serotype 4b complex (4b, 4d, 4e) from two turkey processing plants. | most foodborne outbreaks of listeriosis have been found to involve a small number of closely related strains of listeria monocytogenes serotype 4b. the ecology of these organisms and their reservoirs in nature or in the processing plant environment, however, remain poorly understood. surveys of environmental samples from two turkey processing plants in the united states indicated presence of l. monocytogenes of the serotype 4b complex (serotype 4b and the closely related serotypes 4d and 4e). in ... | 2005 | 16156700 |
| prevalence of antimicrobial resistance genes in listeria monocytogenes isolated from dairy farms. | antimicrobial resistance of listeria monocytogenes (n = 38) isolated from the four dairy farms to 15 antimicrobial agents was evaluated. all 38 l. monocytogenes isolates from the four farms evaluated were resistant to more than one antimicrobial in different combinations. all l. monocytogenes isolates evaluated were resistant to cephalosporin c (minimum inhibitory concentration [mic] > or = 512 microg/ml), streptomycin (mic > or = 32) and trimethoprim (mic > or = 512). most l. monocytogenes isol ... | 2005 | 16156701 |
| cetylpyridinium chloride treatment of ready-to-eat polish sausages: effects on listeria monocytogenes populations and quality attributes. | ready-to-eat polish sausages were inoculated with listeria monocytogenes at either low (3 log(10) cfu/g) or high (7 log(10) cfu/g) levels, treated with a 1% cetylpyridinium chloride (cpc) spray (20 psi, 25 degrees c, 30-sec exposure), vacuum packaged, and stored for 42 days at 0 degrees c or 4 degrees c. non-inoculated samples were similarly treated, packaged, and stored to determine effects on color, firmness, and naturally occurring bacterial populations such as aerobic plate counts (apc). at ... | 2005 | 16156704 |
| predictive modelling and validation of listeria innocua growth at superatmospheric oxygen and carbon dioxide concentrations. | the effect of superatmospheric oxygen and carbon dioxide concentrations on the growth of listeria innocua, which was used as a model organism for the pathogen listeria monocytogenes, was evaluated. the bacteria were grown on a nutrient agar surface at 7 degrees c. three carbon dioxide levels (0%, 12.5% and 25%) were combined with different levels of high oxygen concentrations (above 20%) based on a mixture design. the applied oxygen concentrations did not significantly influence the growth. high ... | 2005 | 16157408 |
| use of rna interference in drosophila s2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. | three genome-wide rna interference screens were performed in drosophila s2 cells to dissect the contribution of host processes to listeria monocytogenes entry, vacuolar escape, and intracellular growth. among the 116 genes identified, several host pathways previously unrecognized as playing a role in listerial pathogenesis were identified: knockdowns affecting vacuolar trafficking to and from the multivesicular body bypassed the requirement for the essential pore-forming toxin listeriolysin o in ... | 2005 | 16157870 |
| a novel role for the lisrk two-component regulatory system in listerial osmotolerance. | understanding how pathogenic bacteria sense and respond to environmental signals, including those involved in triggering virulence gene expression, is a fundamental biological goal. it is now known that the two-component regulatory system lisrk has a novel role in osmosensing and osmoregulation in the intracellular foodborne pathogen listeria monocytogenes. furthermore, htra, a gene linked to osmotolerance and virulence potential in l. monocytogenes, is now known to be under the transcriptional ... | 2005 | 16008610 |
| reflections on the use of robust and least-squares non-linear regression to model challenge tests conducted in/on food products. | in this research, we question the straight-forward use of the classical sum of squared error criterion for identifying the typical parameters of a primary model (like growth rate mumax and lag time lambda) when applied to growth curves obtained in and on food products. firstly, we base our reflections on 62 listeria monocytogenes laboratory challenge tests collected in various environments (broth, crushed cold-smoked salmon, and surface of cold-smoked salmon slices). whereas growth data in broth ... | 2005 | 16009440 |
| [spontaneous bacterial peritonitis caused by listeria monocytogenes. review of one case]. | 2005 | 16013103 | |
| stability and antimicrobial efficiency of eugenol encapsulated in surfactant micelles as affected by temperature and ph. | growth inhibition of four strains of escherichia coli o157:h7 (h1730, f4546, 932, and e0019) and listeria monocytogenes (scott a, 101, 108, and 310) by eugenol encapsulated in water soluble micellar nonionic surfactant solutions (surfynol 485w) adjusted to ph 5, 6, and 7 and incubated at 10, 22, and 32 degrees c was determined. concentrations of eugenol ranged from 0.2 to 0.9% at a surfactant concentration of 5%. antimicrobial activity was assessed using a microbroth dilution assay. eugenol enca ... | 2005 | 16013371 |
| survival of listeria monocytogenes and escherichia coli o157:h7 during sauerkraut fermentation. | sauerkraut was produced from shredded cabbage, as is typical in the united states, and from whole head cabbages, which is a traditional process in parts of eastern europe. the sauerkraut was inoculated with five strain mixtures of escherichia coli o157:h7 and listeria monocytogenes, and the populations of these bacteria, as well as lactic acid bacteria, ph, and titratable acidity, were monitored over the course of fermentation. fermentation variables were temperature (18 and 22 degrees c) and sa ... | 2005 | 16013372 |
| enhancing the bactericidal effect of electrolyzed water on listeria monocytogenes biofilms formed on stainless steel. | biofilms are potential sources of contamination to food in processing plants, because they frequently survive sanitizer treatments during cleaning. the objective of this research was to investigate the combined use of alkaline and acidic electrolyzed (eo) water in the inactivation of listeria monocytogenes biofilms on stainless steel surfaces. biofilms were grown on rectangular stainless steel (type 304, no. 4 finish) coupons (2 by 5 cm) in a 1:10 dilution of tryptic soy broth that contained a f ... | 2005 | 16013373 |
| combined efficacy of nisin and pediocin with sodium lactate, citric acid, phytic acid, and potassium sorbate and edta in reducing the listeria monocytogenes population of inoculated fresh-cut produce. | the inability of chlorine to completely inactivate human bacterial pathogens on whole and fresh-cut produce suggests a need for other antimicrobial washing treatments. nisin (50 microg/ml) and pediocin (100 au/ml) individually or in combination with sodium lactate (2%), potassium sorbate (0.02%), phytic acid (0.02%), and citric acid (10 mm) were tested as possible sanitizer treatments for reducing the population of listeria monocytogenes on cabbage, broccoli, and mung bean sprouts. cabbage, broc ... | 2005 | 16013374 |
| food safety knowledge of consumers and the microbiological and temperature status of their refrigerators. | the objectives of this study were to examine domestic food safety knowledge levels of consumers, establish the levels and incidence of bacterial contamination and operational temperatures in domestic refrigerators, and identify areas in which consumer food safety education is necessary in ireland. a food safety knowledge questionnaire applied to a representative sample of households (n = 1,020) throughout the island of ireland found the gaps in consumer food safety knowledge. analysis of swab sa ... | 2005 | 16013380 |
| microbicidal activity of tripotassium phosphate and fatty acids toward spoilage and pathogenic bacteria associated with poultry. | the ability of solutions of tripotassium phosphate (tpp) and fatty acids (lauric and myristic acids) to reduce populations of spoilage and pathogenic microorganisms associated with processed poultry was examined. in vitro studies were conducted with cultures of bacteria (campylobacter jejuni, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, and staphylococcus aureus) and yeasts (candida ernobii and yarrowia lipolytica). cultures of the bacteria and yeasts ... | 2005 | 16013388 |
| rapid quantitative detection of, listeria monocytogenes in salmon products: evaluation of pre-real-time pcr strategies. | the spread and persistence of listeria monocytogenes in smoked fish products and seafood processing factories are big concerns. thus, the corresponding quality assurance programs must include adequate microbiological control measures. we evaluated eight different pre-pcr sample processing strategies to be coupled with a previously developed real-time pcr assay for the quantitative detection of l. monocytogenes in salmon products. the optimal pre-pcr procedure involved filtration and dna purifica ... | 2005 | 16013389 |
| effect of ph on the inhibition of listeria spp. by vanillin and vanillic acid. | the antimicrobial effects of vanillin and vanillic acid were verified against several species and strains of listeria monocytogenes, listeria innocua, listeria grayi, and listeria seeligeri in a laboratory medium adjusted to ph values ranging from 5.0 to 8.0. medium ph had little influence on the mic of vanillin as determined by a broth dilution assay, and growth of all test strains was inhibited by concentrations ranging from 23 to 33 mm. in contrast, none of the strains were inhibited by 100 m ... | 2005 | 16013390 |
| the influence of mayonnaise ph and storage temperature on the growth of listeria monocytogenes in seafood salad. | seafood salad has been identified as a ready-to-eat food with a relatively high incidence of contamination by listeria monocytogenes; however, little is known about the behavior of this pathogen in seafood salad as a function of product ph and storage temperature. to produce data towards the development of a predictive growth model, a 6-strain cocktail of l. monocytogenes was inoculated onto the surface of a shrimp-crabmeat product, mixed with mayonnaise that was previously adjusted with naoh to ... | 2005 | 16014295 |
| occurrence and ribotypes of listeria monocytogenes in gorgonzola cheeses. | in this study 1656 gorgonzola cheeses, collected from october 2003 to april 2004 in the same industrial plant located in lombardia (italy), were analysed in order to evaluate their level of contamination with listeria monocytogenes after packaging, as well as at the end of the shelf life. a subset of gorgonzola isolates was submitted to automated ecori ribotyping to evaluate their dup-ids (dupont identification library code) and their level of genetic diversity. the isolate ribotyping profiles w ... | 2005 | 16014296 |
| gp96 is a receptor for a novel listeria monocytogenes virulence factor, vip, a surface protein. | by comparative genomics, we have identified a gene of the intracellular pathogen listeria monocytogenes that encodes an lpxtg surface protein absent from nonpathogenic listeria species. this gene, vip, is positively regulated by prfa, the transcriptional activator of the major listeria virulence factors. vip is anchored to the listeria cell wall by sortase a and is required for entry into some mammalian cells. using a ligand overlay approach, we identified a cellular receptor for vip, the endopl ... | 2005 | 16015374 |
| genome-wide rnai screen for host factors required for intracellular bacterial infection. | most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. here, we report a genome-wide rna interference screen to identify host factors required for intracellular bacterial pathogenesis. using drosophila cells and the cytosolic pathogen listeria monocytogenes, we identified 305 double-stranded rnas targeting a wide range of cellular functions that altered l. monocytogenes infection. comparison to a similar screen with mycobacterium fortuitum, a vacuola ... | 2005 | 16020693 |
| pyrrolobenzodiazepine dimers: novel sequence-selective, dna-interactive, cross-linking agents with activity against gram-positive bacteria. | pyrrolo[2,1-c][1,4]benzodiazepine (pbd) dimers are synthetic sequence-selective interstrand dna minor-groove cross-linking agents developed from anthramycins. we investigated the antibacterial activity of three dimers, sjg-136, drg-16 and elb-21, which differ in the structure of the pbd monomeric unit and the length of the linker region between the two identical pbd monomers. | 2005 | 16024592 |
| human mitochondria-derived n-formylated peptides are novel agonists equally active on fpr and fprl1, while listeria monocytogenes-derived peptides preferentially activate fpr. | n-formyl peptides are cleavage products of bacterial and mitochondrial proteins, and can attract leukocytes to sites of infection or tissue damage. in this study, hl-60 cell lines expressing the human n-formyl peptide receptor fpr or its two homologues (fprl1, fprl2) were used to determine the receptor selectivity of n-formylated peptides derived from listeria monocytogenes or from human mitochondrial proteins. bacterial peptides were 100-fold more potent on fpr than on fprl1, whereas none of th ... | 2005 | 16025565 |
| longitudinal studies on listeria monocytogenes and other listeria species in two salmon processing plants. | two plants processing salmon fillets and cold smoked salmon were investigated for occurrence of listeria in products and the environment. analyses were conducted for a period of 31 weeks. at plant a, 252 samples were examined of which 97 were from unprocessed fish and 155 from cold-smoked fish. at plant b, 189 samples of unprocessed fish were investigated. the first examination of unprocessed fish at plant a showed a presence of l. monocytogenes and l. spp. in 81% and 19% of the samples respecti ... | 2005 | 16026018 |
| [effect of the constitutive activity of pathogenicity genes in listeria monocytogenes]. | the effect of the constitutive expression of pathogenicity factors in l. monocytogenes was studied on the model of the intraperitoneal infection of mice. the constitutive expression was due to a single amino acid substitution in the transcriptional factor pfra, the master regulator of l. monocytogenes virulence genes. the effective lethal dose (ld50) for the strain with the constitutive expression of pathogenicity factors was 3 times lower than for the isogenic wildtype strain. when introduced i ... | 2005 | 16028503 |
| the cholesterol-dependent cytolysin listeriolysin o aggregates rafts via oligomerization. | the pore-forming toxin listeriolysin o (llo) is the main virulence factor of listeria monocytogenes. llo is known to act as a pseudo cytokine/chemokine, which induces a broad spectrum of host responses that ultimately influences the outcome of listeriosis. in the present study we demonstrate that llo is a potent aggregator of lipid rafts. llo was found to aggregate the raft associated molecules gm1, the gpi-anchored proteins cd14 and cd16 as well as the tyrosine kinase lyn. abrogation of the cyt ... | 2005 | 16098221 |
| listeria monocytogenes ferritin protects against multiple stresses and is required for virulence. | in this study, the role of listeria monocytogenes ferritin was investigated. the fri gene encoding the ferritin was deleted and the phenotype of the mutant was analyzed demonstrating that ferritin is necessary for optimal growth in minimal medium in both presence and absence of iron, as well as after cold- and heat-shock. we also showed that ferritin provides protection against reactive oxygen species and is essential for full virulence of l. monocytogenes. a comparative proteomic analysis revea ... | 2005 | 16098690 |
| a risk microbiological profile of the australian red meat industry: risk ratings of hazard-product pairings. | a risk profile of microbial hazards across the supply continuum for the beef, sheep and goat meat industries was developed using both a qualitative tool and a semi-quantitative, spreadsheet tool, risk ranger. the latter is useful for highlighting factors contributing to food safety risk and for ranking the risk of various product/pathogen combinations. in the present profile the qualitative tool was used as a preliminary screen for a wide range of hazard-product pairings while risk ranger was us ... | 2005 | 16099063 |
| primary cutaneous listeriosis in a veterinarian. | 2005 | 16100376 | |
| virr, a response regulator critical for listeria monocytogenes virulence. | signature-tagged mutagenesis (stm) was used to identify new genes involved in the virulence of the gram-positive intracellular pathogen listeria monocytogenes. one of the mutants isolated by this technique had the transposon inserted in virr, a gene encoding a putative response regulator of a two-component system. deletion of virr severely decreased virulence in mice as well as invasion in cell-culture experiments. using a transcriptomic approach, we identified 12 genes regulated by virr, includ ... | 2005 | 16102006 |
| the 5' untranslated region-mediated enhancement of intracellular listeriolysin o production is required for listeria monocytogenes pathogenicity. | listeriolysin o (llo) and acta are essential virulence determinants for listeria monocytogenes pathogenesis. transcription of acta and hly, encoding llo, is regulated by prfa and increases dramatically during intracellular infection. the 5' untranslated regions (5' utrs) of acta and prfa have been shown to upregulate expression of their respective gene products. here, we demonstrate that the hly 5' utr plays a critical role in regulating expression of llo during intracellular infection. deletion ... | 2005 | 16102013 |
| gene expression profiling of mouse host response to listeria monocytogenes infection. | the purpose of this study was to evaluate gene expression profiles in the liver and blood for prediction of infection severity from listeria monocytogenes (lm). mice were injected with medium broth (control) or a nonlethal or lethal dose of lm and sacrificed 6 h later. gene expression changes were determined using affymetrix mgu74av2 genechips and confirmed by real-time polymerase chain reaction analysis. we identified discernable genes whose gene expression profiles can be used in pattern recog ... | 2005 | 16102935 |
| solid- and vapor-phase antimicrobial activities of six essential oils: susceptibility of selected foodborne bacterial and fungal strains. | the antimicrobial activity of essential oils (eos) of cinnamon (cinnamon zeylanicum), clove (syzygium aromaticum), basil (ocimum basillicum), rosemary (rosmarinus officinalis), dill (anethum graveolens), and ginger (zingiber officinalis) was evaluated over a range of concentrations in two types of contact tests (solid and vapor diffusion). the eos were tested against an array of four gram-positive bacteria (staphylococcus aureus, bacillus cereus, enterococcus faecalis, and listeria monocytogenes ... | 2005 | 16104824 |
| molecular basis of listeriolysin o ph dependence. | listeriolysin o (llo) is a cholesterol-dependent cytolysin that is an essential virulence factor of listeria monocytogenes. llo pore-forming activity is ph-dependent; it is active at acidic ph (<6), but not at neutral ph. in contrast to other ph-dependent toxins, we have determined that llo pore-forming activity is controlled by a rapid and irreversible denaturation of its structure at neutral ph at temperatures >30 degrees c. rapid denaturation is triggered at neutral ph by the premature unfold ... | 2005 | 16105950 |
| sustained effect of inhaled diesel exhaust particles on t-lymphocyte-mediated immune responses against listeria monocytogenes. | studies have shown that exposure to diesel exhaust particles (dep) suppresses pulmonary host defense against bacterial infection. the present study was carried out to characterize whether dep exposure exerts a sustained effect in which inhaled dep increase the susceptibility of the lung to bacterial infection occurring at a later time. brown norway rats were exposed to filtered air or dep by inhalation at a dose of 21.2 +/- 2.3 mg/m3, 4 h/day for 5 days, and intratracheally instilled with saline ... | 2005 | 16107554 |
| does sporadic listeria gastroenteritis exist? a 2-year population-based survey in nova scotia, canada. | febrile gastroenteritis due to listeria monocytogenes (lm) has been primarily described in foodborne outbreaks. we decided to determine the incidence of sporadic, febrile gastroenteritis due to lm in a large, well-defined north american population over a 2-year period and to compare these cases to sporadic cases of campylobacter and salmonella infections occurring concurrently in the community. | 2005 | 16107973 |
| ability of orally administered ifn-alpha-containing transgenic potato extracts to inhibit listeria monocytogenes infection. | type i interferons (ifn-alpha/beta) were originally thought to be antiviral cytokines, but it has recently been reported that they also play an important role in potentiating innate and adaptive immune responses. moreover, several studies have shown that the oral administration of type i ifn ameliorates various biologic activities. here, we studied the ability of orally administered ifn-alpha to protect mice from systemic listeria monocytogenes infection. daily oral administration of purified na ... | 2005 | 16108729 |
| factors affecting the inactivation of micro-organisms by intense light pulses. | to determine the influence of several factors on the inactivation of micro-organisms by intense light pulses (ilp). | 2005 | 16108787 |
| decrease of enteric micro-organisms from rural sewage sludge during their composting in straw mixture. | to study the decrease of enteric micro-organisms including viable nematode eggs, enteroviruses, faecal indicators (escherichia coli and enterococci) and pathogenic bacteria (listeria monocytogenes, salmonella sp. and clostridium perfringens) of a rural sewage sludge when it is composted for 7 months in mixture with straw. | 2005 | 16108794 |
| screening of benzalkonium chloride resistance in listeria monocytogenes strains isolated during cold smoked fish production. | to determine the susceptibility to disinfectants and cross-resistance to antibiotics in listeria monocytogenes strains isolated from fish products and the fish-processing environment. | 2005 | 16108923 |
| regulation of the bacillus subtilis ytmi operon, involved in sulfur metabolism. | the ytli regulator of bacillus subtilis activates the transcription of the ytmi operon encoding an l-cystine abc transporter, a riboflavin kinase, and proteins of unknown function. the expression of the ytli gene and the ytmi operon was high with methionine and reduced with sulfate. using deletions and site-directed mutagenesis, a cis-acting dna sequence important for ytli-dependent regulation was identified upstream from the -35 box of ytmi. gel mobility shift assays confirmed that ytli specifi ... | 2005 | 16109943 |
| increased vaccine efficacy against tuberculosis of recombinant mycobacterium bovis bacille calmette-guérin mutants that secrete listeriolysin. | the tuberculosis vaccine mycobacterium bovis bacille calmette-guérin (bcg) was equipped with the membrane-perforating listeriolysin (hly) of listeria monocytogenes, which was shown to improve protection against mycobacterium tuberculosis. following aerosol challenge, the hly-secreting recombinant bcg (hly+ rbcg) vaccine was shown to protect significantly better against aerosol infection with m. tuberculosis than did the parental bcg strain. the isogenic, urease c-deficient hly+ rbcg (deltaurec h ... | 2005 | 16110326 |
| functional consequences of genome evolution in listeria monocytogenes: the lmo0423 and lmo0422 genes encode sigmac and lstr, a lineage ii-specific heat shock system. | listeria monocytogenes strains belonging to phylogenetic lineage ii (serotypes 1/2a, 1/2c, and 3a) carry a lineage-specific genome segment encoding a putative sigma subunit of rna polymerase (lmo0423, herein referred to as sigc), a gene of unknown function (lmo0422) similar to the padr family of regulators, and a gene that is similar to the roda-ftsw family of cell wall morphology genes (lmo0421). to understand the function of this set of genes, their expression patterns and the effects of null ... | 2005 | 16237008 |