Publications

diminution of arsenic accumulation in rice seedlings co-cultured with anabaena sp.: modulation in the expression of lower silicon transporters, two nitrogen dependent genes and lowering of antioxidants activity.	the present study was intended to investigate the role of algae, anabaena sp. in the amelioration of as toxicity, when co-cultured with rice seedlings. the reduction of growth in rice seedlings against as(iii) and as(v) was recovered with anabaena sp. the anabaena sp. also reduced the accumulation of as, where it was more efficient against 60µm as(iii) (49%) than as(v) (23%) in rice shoot. similarly, with reduction of as accumulation, lower silicon transporters (lsi-1 and lsi-2) was found to be ...	2018	29331724
contrasting effects of alkaline amendments on the bioavailability and uptake of cd in rice plants in a cd-contaminated acid paddy soil.	reducing cadmium (cd) concentrations in rice grains is important for food safety, particularly in acid paddy fields in south china where the soils have been previously contaminated with cd. a field experiment was conducted to evaluate the effects of four alkaline amendments, i.e., lime, compost, biochar, and carbide slag on soil bioavailability and uptake of cd in plants of two rice cultivars (oryza sativa l.) in a cd-contaminated acid paddy soil. the addition of these amendments significantly d ...	2018	29330814
genome-wide analyses of direct target genes of four rice nac-domain transcription factors involved in drought tolerance.	plant stress responses and mechanisms determining tolerance are controlled by diverse sets of genes. transcription factors (tfs) have been implicated in conferring drought tolerance under drought stress conditions, and the identification of their target genes can elucidate molecular regulatory networks that orchestrate tolerance mechanisms.	2018	29329517
t-dna tagging-based gain-of-function of oshkt1;4 reinforces na exclusion from leaves and stems but triggers na toxicity in roots of rice under salt stress.	the high affinity k⁺ transporter 1;4 (hkt1;4) in rice (oryza sativa), which shows na⁺ selective transport with little k⁺ transport activity, has been suggested to be involved in reducing na in leaves and stems under salt stress. however, detailed physiological roles of oshkt1;4 remain unknown. here, we have characterized a transfer dna (t-dna) insertion mutant line of rice, which overexpresses oshkt1;4, owing to enhancer elements in the t-dna, to gain an insight into the impact of oshkt1;4 on sa ...	2018	29329278
a maize phytochrome-interacting factors protein zmpif1 enhances drought tolerance by inducing stomatal closure and improves grain yield in oryza sativa.	phytochrome-interacting factors (pifs) play major roles in regulating plant growth and development, but their roles in drought stress remain elusive. here, we cloned and characterized a maize (zea mays) pif transcription factor, zmpif1. the expression level of zmpif1 was significantly induced by independent drought and abscisic acid (aba) treatments. the zmpif1 transgenic rice and arabidopsis displayed water-saving and drought-resistance, which were associated with reduced a stomatal aperture an ...	2018	29327440
a novel rice xylosyltransferase catalyzes the addition of 2-o-xylosyl side chains onto the xylan backbone.	xylan is a major hemicellulose in both primary walls and secondary walls of grass species. it consists of a linear backbone of β-1,4-linked xylosyl residues that are often substituted with monosaccharides and disaccharides. xylosyl substitutions directly on the xylan backbone have not been reported in grass species and genes responsible for xylan substitutions in grass species have not been well elucidated. here, we report functional characterization of a rice (oryza sativa) gt61 glycosyltransfe ...	2018	29325159
strigolactone biosynthesis genes of rice is required for the punctual entry of arbuscular mycorrhizal fungi into the roots.	arbuscular mycorrhiza (am) is a mutualistic association between most plant species and the ancient fungal phylum glomeromycota in roots, and it plays a key role in a plant's nutrient uptake from the soil. roots synthesize strigolactones (sls), derivatives of carotenoids, and exude them to induce energy metabolism and hyphal branching of am fungi. despite the well-documented roles of sls in the presymbiotic phase, little is known about the role of sls in the process of root colonization. here we ...	2018	29325120
protein activity of the fusarium fujikuroi rhodopsins caro and opsa and their relation to fungus-plant interaction.	fungi possess diverse photosensory proteins that allow them to perceive different light wavelengths and to adapt to changing light conditions in their environment. the biological and physiological roles of the green light-sensing rhodopsins in fungi are not yet resolved. the rice plant pathogen fusarium fujikuroi exhibits two different rhodopsins, caro and opsa. caro was previously characterized as a light-driven proton pump. we further analyzed the pumping behavior of caro by patch-clamp experi ...	2018	29324661
silicon amendment to rice plants contributes to reduced feeding in a phloem-sucking insect through modulation of callose deposition.	silicon (si) uptake by poaceae plants has beneficial effects on herbivore defense. increased plant physical barrier and altered herbivorous feeding behaviors are documented to reduce herbivorous arthropod feeding and contribute to enhanced plant defense. here, we show that si amendment to rice (oryza sativa) plants contributes to reduced feeding in a phloem feeder, the brown planthopper (nilaparvata lugens, bph), through modulation of callose deposition. we associated the temporal dynamics of bp ...	2018	29321899
coexistence of bt-transgenic and conventional rice affects insect abundance and plant fitness in fields.	as genetically modified (gm) crops have been cultivated worldwide, concerns emerge over ecological consequences because of the coexistence of transgenic and non-transgenic crops in fields. we first conducted field experiments using insect-resistant bt-transgenic rice expressing bacillus thuringiensis and its counterpart conventional rice (oryza sativa l.) with or without insecticide spraying in 2013 and 2014. in 2015 and 2016, bt-transgenic and conventional rice were employed in the pure and mix ...	2018	29318739
involvement of nadph oxidase isoforms in the production of o2- manipulated by aba in the senescing leaves of early-senescence-leaf (esl) mutant rice (oryza sativa).	in this study, the differences in reactive oxygen species (ros) generation and abscisic acid (aba) accumulation in senescing leaves were investigated by early-senescence-leaf (esl) mutant and its wild type, to clarify the relationship among aba levels, ros generation, and nadph oxidase (nox) in senescing leaves of rice (oryza sativa). the temporal expression levels of osnox isoforms in senescing leaves and their expression patterns in response to aba treatment were determined through quantitativ ...	2018	29309410
characterization of new bioactive enzyme inhibitors from endophytic bacillus amyloliquefaciens rwl-1.	endophytic bacteria are known to produce a wide array of bioactive secondary metabolites with beneficial effects on human health. in the current study, a novel endophytic bacterial strain, bacillus amyloliquefaciens rwl-1, was isolated from the seeds of oryza sativa. initially, the crude extract of rwl-1 was assessed for potential biological effects of enzyme inhibition and cytotoxicity and was found to exhibit a broad spectrum inhibition for α-glucosidase (37 ± 0.09%) and urease (49.4 ± 0.53%). ...	2018	29304029
multiple origin but single domestication led to oryza sativa.	the domestication scenario that led to asian rice (oryza sativa) is a contentious topic. here, we have reanalyzed a previously published large-scale wild and domesticated rice dataset, which were also analyzed by two studies but resulted in two contrasting domestication models. we suggest the analysis of false positive selective sweep regions and phylogenetic analysis of concatenated genomic regions may have been the sources that contributed to the different results. in the end, our result indic ...	2018	29301862
exogenous short-term silicon application regulates macro-nutrients, endogenous phytohormones, and protein expression in oryza sativa l.	silicon (si) has been known to regulate plant growth; however, the underlying mechanisms of short-term exogenous si application on the regulation of calcium (ca) and nitrogen (n), endogenous phytohormones, and expression of essential proteins have been little understood.	2018	29301510
identification and comparative analysis of premature senescence leaf mutants in rice (oryza sativa l.).	premature leaf senescence negatively impacts the grain yield in the important monocot rice (oryza sativa l.); to understand the molecular mechanism we carried out a screen for mutants with premature senescence leaves in a mutant bank generated by ethyl methane sulfonate (ems) mutagenesis of elite indica rice zhongjian100. five premature senescence leaf (psl15, psl50, psl89, psl117 and psl270) mutants were identified with distinct yellowish phenotypes on leaves starting from the tillering stage t ...	2018	29301377
sequencing of bulks of segregants allows dissection of genetic control of amylose content in rice.	amylose content (ac) is a key quality trait in rice. a cross between oryza glaberrima (african rice) and oryza sativa (asian rice) segregating for ac was analysed by sequencing bulks of individuals with high and low ac. snp associated with the granule bound starch synthase (gbss1) locus on chromosome 6 were polymorphic between the bulks. in particular, a g/a snp that would result in an asp to asn mutation was identified. this amino acid substitution may be responsible for differences in gbss act ...	2018	28499072
acyl-coa-binding protein family members in laticifers are possibly involved in lipid and latex metabolism of hevea brasiliensis (the para rubber tree).	acyl-coa-binding proteins (acbps) are mainly involved in acyl-coa ester binding and trafficking in eukaryotic cells, and their various functions have been characterized in model plants, such as arabidopsis thaliana (a. thaliana), oryza sativa (rice), and other plant species. in the present study, genome-wide mining and expression analysis of acbp genes was performed on hevea brasiliensis (the para rubber tree), the most important latex-producing crop in the world.	2018	29295704
regulation of sucrose transporters and phloem loading in response to environmental cues.	suc transporters (suts) play a key role in the allocation and partitioning of photosynthetically fixed carbon in plants. while a function could be assigned to many members of the sut family, almost no information is available on their regulation. here, the transcriptional regulation of suts in response to various environmental stimuli in the leaves of five dicots (arabidopsis [arabidopsis thaliana], soybean [glycine max], potato [solanum tuberosum], tomato [solanumlycopersicum], and poplar [popu ...	2018	29158330
pan-genome analysis highlights the extent of genomic variation in cultivated and wild rice.	the rich genetic diversity in oryza sativa and oryza rufipogon serves as the main sources in rice breeding. large-scale resequencing has been undertaken to discover allelic variants in rice, but much of the information for genetic variation is often lost by direct mapping of short sequence reads onto the o. sativa japonica nipponbare reference genome. here we constructed a pan-genome dataset of the o. sativa-o. rufipogon species complex through deep sequencing and de novo assembly of 66 divergen ...	2018	29335547
overcoming the species hybridization barrier by ploidy manipulation in the genus oryza.	in most eudicot and monocot species, interspecific and interploidy crosses generally display abnormalities in the endosperm that are the major cause of a post-zygotic hybridization barrier. in some eudicot species, however, this type of hybridization barrier can be overcome by the manipulation of ploidy levels of one parental species, suggesting that the molecular mechanisms underlying the species hybridization barrier can be circumvented by genome dosage. we previously demonstrated that endospe ...	2018	29271099
rice nucleosome patterns undergo remodeling coincident with stress-induced gene expression.	formation of nucleosomes along eukaryotic dna has an impact on transcription. major transcriptional changes occur in response to low external phosphate (pi) in plants, but the involvement of chromatin-level mechanisms in pi starvation responses have not been investigated.	2018	29373953
biochar amendment changes jasmonic acid levels in two rice varieties and alters their resistance to herbivory.	biochar addition to soil not only sequesters carbon for the long-term but enhances agricultural productivity. several well-known benefits arise from biochar amendment, including constant provision of nutrients, increased soil moisture retention, decreased soil bulk density, and sometimes the induction of systemic resistance against foliar and soil borne plant pathogens. however, no research has investigated the potential of biochar to increase resistance against herbivory. the white-backed plant ...	2018	29373575
characterization and oral delivery of proinsulin-transferrin fusion protein expressed using expresstec.	proinsulin-transferrin fusion protein (proins-tf) has been designed and successfully expressed from the mammalian hek293 cells (hek-proins-tf). it was found that hek-proins-tf could be converted into an activated form in the liver. furthermore, hek-proins-tf was demonstrated as an extra-long acting insulin analogue with liver-specific insulin action in streptozotocin (stz)-induced type 1 diabetic mice. however, due to the low production yield from transfected hek293 cells, there are other intere ...	2018	29373562
exploring traditional aus-type rice for metabolites conferring drought tolerance.	traditional varieties and landraces belonging to the aus-type group of rice (oryza sativa l.) are known to be highly tolerant to environmental stresses, such as drought and heat, and are therefore recognized as a valuable genetic resource for crop improvement. using two aus-type (dular, n22) and two drought intolerant irrigated varieties (ir64, ir74) an untargeted metabolomics analysis was conducted to identify drought-responsive metabolites associated with tolerance.	2018	29372429
draft genome sequence of pantoea ananatis strain 1.38, a bacterium isolated from the rhizosphere of oryza sativa var. puntal that shows biotechnological potential as an inoculant.	pantoea ananatis 1.38 is a strain isolated from the rhizosphere of irrigated rice in southern spain. its genome was estimated at 4,869,281 bp, with 4,644 coding sequences (cdss). the genome encompasses several cdss related to plant growth promotion, such as that for siderophore metabolism, and virulence genes characteristic of pathogenic pantoea spp. are absent.	2018	29371365
chlorophyll fluorescence and reflectance-based non-invasive quantification of blast, bacterial blight and drought stresses in rice.	response of rice (oryza sativa) exposed to both biotic and abiotic stresses can be quantified by employing fast and accurate optical methods. in this study, the overall stress responses of (i) 12 near-isogenic lines (nils) in the genetic background of the rice blast-susceptible cultivar lijiangxintuanheigu (lth) and (ii) four nils in the genetic background of the bacterial blight-susceptible cultivar ir24, were inspected by means of chl fluorescence (chl-f) imaging. the distribution of the maxim ...	2018	29370434
progress in zip transporter gene family in rice.	zinc and iron are essential mineral elements for the growth of oryza sativa l. and also micronutrients for human health. therefore, it is vital to study biofortification of rice with zn and fe in order to improve the yield and quality of rice, as well as to enhance nutritional states of humans. the zinc-regulated transporters and iron-regulated transporter-like proteins (the zip family) control the absorption and translocation of zn and fe and maintain their homeostasis in rice. reciprocally, th ...	2018	29367191
overexpression of osmir164b-resistant osnac2 improves plant architecture and grain yield in rice.	plant architecture is a major target of rice (oryza sativa) breeding and selection, but the underlying regulatory networks remain unclear. here, we overexpressed a osnac2 mutant (oern) that cannot be cleaved by the microrna mir164b. oern plants had better plant architecture, longer panicles, and produced more grains. the parental line averaged 12.2 primary and 31.5 secondary branches in the main panicles; two oern lines averaged 15.0 and 15.2 primary, and 41.5 and 44.3 secondary branches. in lar ...	2018	29365136
osmads57 together with ostb1 coordinates transcription of its target oswrky94 and d14 to switch its organogenesis to defense for cold adaptation in rice.	plants modify their development to adapt to their environment, protecting themselves from detrimental conditions such as chilling stress by triggering a variety of signaling pathways; however, little is known about how plants coordinate developmental patterns and stress responses at the molecular level. here, we demonstrate that interacting transcription factors osmads57 and ostb1 directly target the defense gene oswrky94 and the organogenesis gene d14 to trade off the functions controlling/mode ...	2018	29364524
the newly identified heat-stress sensitive albino 1 gene affects chloroplast development in rice.	high temperature, a major abiotic stress, significantly affects the yield and quality of crops in many parts of the world. components of the photosynthetic apparatus are highly susceptible to thermal damage. although the responses to acute heat stress have been studied intensively, the mechanisms that regulate chloroplast development under heat stress remain obscure, especially in crop plants. here, we cloned and characterized the gene responsible for the heat-sensitive albino1 (hsa1) mutation i ...	2018	29362095
the c-s-a gene system regulates hull pigmentation and reveals evolution of anthocyanin biosynthesis pathway in rice.	floral organs in rice (oryza sativa) can be purple, brown or red in color due to the accumulation of flavonoids. but the molecular mechanism underlying specific organ pigmentation is not clear. here, we propose a c-s-a gene model for rice hull pigmentation and characterize through genetic, molecular and metabolomic approaches. further we conduct phylogenetic studies to reveal the evolution of rice color. in this gene system, c1 encodes a r2r3-myb transcription factor and acts as a color-producin ...	2018	29361187
a negative regulator in response to salinity in rice: oryza sativa salt-, aba- and drought-induced ring finger protein 1 (ossadr1).	ring (really interesting new gene) finger proteins play crucial roles in abiotic stress responses in plants. we report the ring finger e3 ligase, an oryza sativa salt, abscisic acid (aba), and drought stress-induced ring finger protein 1 gene (ossadr1). we demonstrated that although ossar1 possesses e3 ligase activity, a single amino acid substitution (ossadr1c168a) in the ring domain exhibited no e3 ligase activity, suggesting that the activity of most e3s is specified by the ring domain. addit ...	2018	29361060
lack of actpk1, an sty kinase, enhances ammonium uptake and use and promotes growth of rice seedlings under sufficient external ammonium.	ammonium influx into plant roots via the high-affinity transport system (hats) is down-modulated under elevated external ammonium, preventing ammonium toxicity. in ammonium-fed arabidopsis, ammonium transporter 1 (amt1) trimers responsible for hats activity are allosterically inactivated in a dose-dependent manner via phosphorylation of the conserved threonine at the carboxyl-tail by the calcineurin b-like protein 1-calcineurin b-like protein-interacting protein kinase 23 complex and other yet u ...	2018	29356222
plant s6 kinases do not require hydrophobic motif phosphorylation for activity in yeast lacking ypk3.	the ribosomal protein s6 kinases (s6k) are among the major substrates and crucial effectors of the target of rapamycin (tor) kinase, which is an evolutionarily conserved regulator of cell growth and proliferation. recent research indicates that yeast ypk3 is an ortholog of mammalian s6ks. here, we find that plant s6ks restore ribosomal protein s6 phosphorylation in a rapamycin-sensitive manner in yeast cells lacking ypk3. however, phosphorylation of a hydrophobic motif, which is mediated through ...	2018	29355926
identification of cyp703a3-3 and analysis of regulatory role of cyp703a3 in rice anther cuticle and pollen exine development.	anther cuticle and pollen exine are two elaborated lipid-soluble barriers protecting pollen grains from environmental and biological stresses. however, less is known about the mechanisms underlying the synthesis of these lipidic polymers. here, we identified a no-pollen male-sterility mutant cyp703a3-3 from indica restorer line zhonghui 8015 (zh8015) mutant library treated with 60coγ-ray radiation. histological analysis indicated that cyp703a3-3 underwent abnormal tapetal cells development, prod ...	2018	29355682
isovitexin (iv) induces apoptosis and autophagy in liver cancer cells through endoplasmic reticulum stress.	liver cancer is a leading cause of cancer death worldwide, and novel chemotherapeutic drugs to suppress liver cancer are urgently required. isovitexin (iv), a glycosylflavonoid, is extracted from rice hulls of oryza sativa, and has various biological activities. however, the anti-tumor effect of iv against liver cancer has not yet been demonstrated in vitro or in vivo. in the present study, we showed that iv significantly suppressed the growth of liver cancer cells. mechanistic studies indicated ...	2018	29355527
expression analysis of proteinase inhibitor-ii under osrglp2 promoter in response to wounding and signaling molecules in transgenic nicotiana benthamiana.	proteinase inhibitor-ii (pi-ii) genes are important defense related genes that play critical regulatory roles in plant growth and development. in the present study, the expression of tomato pi-ii gene was investigated under the control of a wound-inducible osrglp2 (oryza sativa root germin like protein 2) promoter in transgenic tobacco plants after wounding, aba and meja applications. transcript level of target gene in transgenic plants was confirmed by quantitative real time pcr (qpcr). in resp ...	2018	29354362
evolution of the spx gene family in plants and its role in the response mechanism to phosphorus stress.	molecular and genomic studies have shown the presence of a large number of spx gene family members in plants, some of which have been proved to act in p signalling and homeostasis. in this study, the molecular and evolutionary characteristics of the spx gene family in plants were comprehensively analysed, and the mechanisms underlying the function of spx genes in p signalling and homeostasis in the model plant species arabidopsis (arabidopsis thaliana) and rice (oryza sativa), and in important c ...	2018	29298909
mutmapplus identified novel mutant alleles of a rice starch branching enzyme iib gene for fine-tuning of cooked rice texture.	physicochemical properties of storage starch largely determine rice grain quality and food characteristics. therefore, modification of starch property is effective to fine-tune cooked rice textures. to obtain new resources with modified starch property as breeding materials, we screened a mutant population of a japonica cultivar nipponbare and found two independent mutant lines, altered gelatinization (age)1 and age2, with moderate changes in starch gelatinization property. a combination of conv ...	2018	28499068
comparative proteomics and protein profile related to phenolic compounds and antioxidant activity in germinated oryza sativa 'kdml105' and thai brown rice 'mali daeng' for better nutritional value.	brown rice (br) and germinated brown rice (gbr) are considered as prime sources of carbohydrate and bioactive compounds for more than half of the populations worldwide. several studies have reported on the proteomics of br and gbr; however, the proteomic profiles related to the synthesis of bioactive compounds are less well documented. in the present study, br and gbr were used in a comparative analysis of the proteomic and bioactive compound profiles for two famous thai rice varieties: khao daw ...	2018	28646518
variable silicon accumulation in plants affects terrestrial carbon cycling by controlling lignin synthesis.	current climate and land-use changes affect regional and global cycles of silicon (si), with yet uncertain consequences for ecosystems. the key role of si in marine ecology by controlling algae growth is well recognized but research on terrestrial ecosystems neglected si since not considered an essential plant nutrient. however, grasses and various other plants accumulate large amounts of si, and recently it has been hypothesized that incorporation of si as a structural plant component may subst ...	2018	28755386
an osmotin from the resurrection plant tripogon loliiformis (tlosm) confers tolerance to multiple abiotic stresses in transgenic rice.	osmotin is a key protein associated with abiotic and biotic stress response in plants. in this study, an osmotin from the resurrection plant tripogon loliiformis (tlosm) was characterized and functionally analyzed under abiotic stress conditions in t. loliiformis as well as in transgenic nicotiana tabacum (tobacco) and oryza sativa (rice) plants. real-time pcr analysis on mixed elicitor cdna libraries from t. loliiformis showed that tlosm was upregulated a 1000-fold during the early stages of os ...	2018	28466470
relative salinity tolerance of rice cultivars native to north east india: a physiological, biochemical and molecular perspective.	salinity is the second most prevalent abiotic stress faced by plants, and rice is not an exception. through this study, it has been tried upon, to study the relative salinity tolerance of eight local varieties of north east india. preliminary screening was based on their dose- and time-dependent physiological responses to salinity stress. among the cultivars, tampha was found to be relatively more tolerant, whereas mse9 the most sensitive. to further ascertain their tolerance capacity, mda and h ...	2018	28718009
field experiment for determining lead accumulation in rice grains of different genotypes and correlation with iron oxides deposited on rhizosphere soil.	paddy rice (oryza sativa l.) is a major staple crop in asia. however, heavy metal accumulation in paddy soil poses a health risk for rice consumption. although plant uptake of pb is usually low, pb concentrations in rice plants have been increasing with pb contamination in paddy fields. it is known that iron oxide deposits in the rhizosphere influence the absorption of soil pb by rice plants. in this study, 14 rice cultivars bred in taiwan, including ten japonica cultivars (hl21, kh145, tc192, t ...	2018	28826122
do aeration conditions affect arsenic and phosphate accumulation and phosphate transporter expression in rice (oryza sativa l.)?	widespread contamination of rice with arsenic (as) has revealed a major exposure pathway to humans. the present study aimed to investigate the effects of oxygen in the rhizosphere on phosphate (p) transporter (for arsenate transportation) expressions, on as and p accumulation and as speciation in four rice genotypes. oxygenation marginally increased root and shoot length. total as concentrations in rice roots were dramatically reduced following aeration compared to stagnant treatments (p < 0.001 ...	2018	27798801