Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| experimental evaluation of different precision criteria applicable to microbiological counting methods. | the dispersion of microbiological counting measurements, when repeating the analysis on the same material both within a laboratory (repeatability) and between laboratories (reproducibility) can be characterized by the organization of interlaboratory studies, where several sets of identical test materials are sent to several laboratories. using the example of data generated by an interlaboratory study on enumeration of listeria monocytogenes in foods by the standardized reference method (colony-c ... | 2005 | 16001859 |
| cpg oligonucleotides partially inhibit growth of mycobacterium tuberculosis, but not salmonella or listeria, in human monocyte-derived macrophages. | immunostimulatory dna sequences and their synthetic oligonucleotide analogs (cpg-odn) activate innate immunity and can stimulate antibacterial effects against numerous intracellular pathogens. while it has been shown previously that cpg-odn inhibit growth of mycobacterium avium in murine and human macrophages, we now report that mycobacterium tuberculosis growth can be inhibited by cpg-odn treatment of human monocyte-derived macrophages (hmdm). this inhibitory effect was reversed by ifn-gamma, w ... | 2005 | 16002269 |
| hepatocytes express abundant surface class i mhc and efficiently use transporter associated with antigen processing, tapasin, and low molecular weight polypeptide proteasome subunit components of antigen processing and presentation pathway. | hepatic expression levels of class i mhc ags are generally regarded as very low. because the status of these ags and their ability to present peptides are important for the understanding of pathogen clearance and tolerogenic properties of the liver, we set out to identify the factors contributing to the reported phenotype. unexpectedly, we found that the surface densities of k(b) and d(b) on c57bl/6 mouse hepatocytes are nearly as high as on splenocytes, as are the lysate concentrations of mrna ... | 2005 | 16002705 |
| invariant v alpha 14+ nkt cells participate in the early response to enteric listeria monocytogenes infection. | invariant valpha14(+) nkt cells are a specialized cd1-reactive t cell subset implicated in innate and adaptive immunity. we assessed whether valpha14(+) nkt cells participated in the immune response against enteric listeria monocytogenes infection in vivo. using cd1d tetramers loaded with the synthetic lipid alpha-galactosylceramide (cd1d/alphagc), we found that splenic and hepatic valpha14(+) nkt cells in c57bl/6 mice were early producers of ifn-gamma (but not il-4) after l. monocytogenes infec ... | 2005 | 16002715 |
| effect of inoculum size on the combined temperature, ph and aw limits for growth of listeria monocytogenes. | the growth/no growth responses of listeria monocytogenes inoculated at four levels (0.90, 2.58, 4.20 and 6.81 log cfu/well; 300 microl medium/well) into tryptic soy broth (tsb) were monitored at different combinations of temperature (4 to 30 degrees c), ph (3.76 to 6.44) and aw (0.888 to 0.997) for 60 days. the study was conducted in 96-well microtiter plates and growth was monitored visually and by recording the turbidity of the medium with an automated microplate reader. the growth limits of t ... | 2005 | 16005535 |
| treatment of brain abscess due to listeria monocytogenes. | 2005 | 16007552 | |
| listeriolysin o-induced membrane permeation mediates persistent interleukin-6 production in caco-2 cells during listeria monocytogenes infection in vitro. | listeriolysin o (llo), a major virulence factor of listeria monocytogenes, is a member of the cholesterol-dependent cytolysin family and plays important roles not only in survival of this bacterium in phagocytes but also in induction of various cellular responses, including cytokine production. in this work, we examined the involvement of llo in induction of the cytokine response in intestinal epithelial cells, the front line of host defense against food-borne listeriosis. infection of caco-2 ce ... | 2005 | 15972472 |
| infection-stimulated fibrin deposition controls hemorrhage and limits hepatic bacterial growth during listeriosis. | bacterial infections are major causes of human mortality. the activation of coagulation pathways leading to the deposition of insoluble fibrin frequently accompanies bacterial infection, and much attention has focused upon the pathological attributes of infection-stimulated fibrin deposition. nevertheless, here we present conclusive evidence that infection-stimulated fibrin deposition can perform critical protective functions during bacterial infection. specifically, we demonstrate that coagulat ... | 2005 | 15972474 |
| involvement of listeria monocytogenes phosphatidylinositol-specific phospholipase c and host protein kinase c in permeabilization of the macrophage phagosome. | we have previously shown that phosphatidylinositol-specific phospholipase c (pi-plc) produced by listeria monocytogenes activates a host protein kinase c (pkc) cascade which promotes escape of the bacterium from a macrophage-like cell phagosome. here, we provide evidence linking bacterial pi-plc and host pkc beta to phagosome permeabilization, which precedes escape. | 2005 | 15972539 |
| cross-recognition of n-formylmethionine peptides is a general characteristic of h2-m3-restricted cd8+ t cells. | h2-m3-restricted cd8+ t cells can exhibit cross-reactivity to different bacterially derived n-formylmethionine peptides. the extent of this promiscuity is unclear. we deleted the nonredundant fmivtlf epitope and found that listeria monocytogenes still primed fmivtlf-specific t cells. thus, cross-reactivity appears to be a more general characteristic of h2-m3-restricted t cells. | 2005 | 15972542 |
| cd8alpha+ dendritic cells selectively present mhc class i-restricted noncytolytic viral and intracellular bacterial antigens in vivo. | cd8alpha(+) dendritic cells (dcs) have been shown to be the principal dc subset involved in priming mhc class i-restricted ctl immunity to a variety of cytolytic viruses, including hsv type 1, influenza, and vaccinia virus. whether priming of ctls by cd8alpha(+) dcs is limited to cytolytic viruses, which may provide dead cellular material for this dc subset, or whether these dcs selectively present intracellular ags, is unknown. to address this question, we examined ag presentation to a noncytol ... | 2005 | 15972648 |
| simultaneous th1-type cytokine expression is a signature of peritoneal cd4+ lymphocytes responding to infection with listeria monocytogenes. | the robust murine response to infection with listeria monocytogenes makes an excellent model to study the functional development of immune cells. we investigated the cellular immune response to i.p. infection using intracellular cytokine staining to identify ag-specific lymphocytes. cd4(+) peritoneal exudate cells obtained 10 days postinfection predominantly coexpressed tnf-alpha, ifn-gamma, and il-2 after polyclonal or ag stimulation. a population of cells simultaneously making tnf-alpha and if ... | 2005 | 15972673 |
| listeria-infected myeloid dendritic cells produce ifn-beta, priming t cell activation. | the intracellular bacterium listeria monocytogenes infects dendritic cells (dc) and other apcs and induces potent cell-mediated protective immunity. however, heat-killed bacteria fail to do so. this study explored whether dc differentially respond to live and killed listeria and how this affects t cell activation. to control for bacterial number, a replication-deficient strain, lmdd, defective in d-alanine biosynthesis, was used. we found that dc internalize both live and heat-killed lmdd and si ... | 2005 | 15972676 |
| evolution of listeria populations in food samples undergoing enrichment culturing. | the isolation of listeria monocytogenes from food is carried out using a double enrichment. it is believed that the double enrichment can allow the overgrowth of listeria innocua in samples where both species are present. in this study, we have evaluated the impact of overgrowth between listeria species and strains during each step of the enrichment process. the effect of factors minimizing interactions between strains or phage inhibitory effects has also been estimated. in an artificially conta ... | 2005 | 15975676 |
| autistic effector t cells in mice with a point mutation in the lat adaptor fail to respond to listeria monocytogenes infection. | the adaptor protein linker for activation of t cells (lat) is an important transducer of extracellular t cell stimuli. in mice with a point mutation in lat (laty136f), tcr signaling is substantially compromised and laty136f t cells are unresponsive to cd3 cross-linking in vitro. nevertheless, laty136f mice develop a polyclonal lymphoproliferation of cd4(+) t cells, which display a t(h)2-polarized effector phenotype. in this study, laty136f mice were infected with the intracellular bacterium list ... | 2005 | 15976034 |
| prediction of pathogen growth on iceberg lettuce under real temperature history during distribution from farm to table. | the growth of pathogenic bacteria escherichia coli o157:h7, salmonella spp., and listeria monocytogenes on iceberg lettuce under constant and fluctuating temperatures was modelled in order to estimate the microbial safety of this vegetable during distribution from the farm to the table. firstly, we examined pathogen growth on lettuce at constant temperatures, ranging from 5 to 25 degrees c, and then we obtained the growth kinetic parameters (lag time, maximum growth rate (micro(max)), and maximu ... | 2005 | 15979180 |
| an outbreak of food-borne listeriosis due to cheese in japan, during 2001. | food-borne outbreaks caused by listeria monocytogenes have been recognized in us and european countries. only sporadic cases, of neonatal listeriosis, have been reported in japan. since l. monocytogenes has been often isolated from foods in japan, food-borne outbreaks potentially could have occurred. in february 2001, l. monocytogenes serotype 1/2b was isolated from a washed-type cheese during routine listeria monitoring of 123 domestic cheeses. further samples from products and the environments ... | 2005 | 15979181 |
| effect of inoculation of carnobacterium divergens v41, a bio-preservative strain against listeria monocytogenes risk, on the microbiological, chemical and sensory quality of cold-smoked salmon. | the aim of this study was to develop a bio-preservation strategy for cold-smoked salmon (css) by the use of lactic acid bacteria previously selected for their capability to inhibit the growth of listeria monocytogenes in the product. the spoiling potential of three carnobacterium strains (carnobacterium divergens v41, carnobacterium piscicola v1 and sf668) was tested in sterile css blocks inoculated by 10(4-5) cfu g(-)(1) and stored under vacuum for 9 days at 4 degrees c followed by 19 days at 8 ... | 2005 | 15979753 |
| bacterial shape and acta distribution affect initiation of listeria monocytogenes actin-based motility. | we have examined the process by which the intracellular bacterial pathogen listeria monocytogenes initiates actin-based motility and determined the contribution of the variable surface distribution of the acta protein to initiation and steady-state movement. to directly correlate acta distributions to actin dynamics and motility of live bacteria, acta was fused to a monomeric red fluorescent protein (mrfp1). actin comet tail formation and steady-state bacterial movement rates both depended on ac ... | 2005 | 15980176 |
| bioluminescent imaging of melanoma in live mice. | melanoma is highly resistant to conventional chemotherapeutic agents and novel therapeutic approaches are needed. current animal models of melanoma in animals are sub-optimal. the most commonly used homograft model is the b16 mouse melanoma. evaluation of potential melanoma therapies with this model is limited by the inaccuracy of caliper measurement of subcutaneous tumors, of counting lung nodules in metastasis models, and the indirect nature of "survival" curves when studying brain metastases. ... | 2005 | 15982316 |
| production of antibodies to canine il-1beta and canine tnf to assess the role of proinflammatory cytokines. | il-1 and tnf are important proinflammatory cytokines implicated in both antimicrobial host defense and pathogenesis of diseases with an immune-mediated and/or inflammatory component. respective studies in the dog have been hampered by the unavailability of reagents allowing the specific measurement of canine cytokine proteins and the effect of canine cytokine neutralization by ab. starting with recombinant canine (rcan) il-1beta and rcantnf, four polyclonal antisera and 22 mab specific for rcani ... | 2005 | 15982477 |
| tumor necrosis factor and its blockade in granulomatous infections: differential modes of action of infliximab and etanercept? | tumor necrosis factor (tnf) is a critical component of both the antibacterially protective and the inflammatory responses against infections, particularly infections with intracellularly viable microorganisms. it is, therefore, not surprising that some treatment regimens that target tnf function have resulted in an increase in complications associated with infections due to such pathogens as mycobacterium tuberculosis, listeria monocytogenes, and histoplasma capsulatum; organized granuloma forma ... | 2005 | 15983900 |
| the host type i interferon response to viral and bacterial infections. | type i interferons (ifn) are well studied cytokines with anti-viral and immune-modulating functions. type i ifns are produced following viral infections, but until recently, the mechanisms of viral recognition leading to ifn production were largely unknown. toll like receptors (tlrs) have emerged as key transducers of type i ifn during viral infections by recognizing various viral components. furthermore, much progress has been made in defining the signaling pathways downstream of tlrs for type ... | 2005 | 15987599 |
| anti-listeria monocytogenes bacteriocin-like inhibitory substances from enterococcus faecium uq31 isolated from artisan mexican-style cheese. | artisan fresh mexican-style cheeses are commonly made from raw milk that provides not only rich flavors, but also a diversity of associated lactic acid bacteria (lab) strains. enterococcus faecium uq31 was isolated from panela cheese and produced bacteriocin-like inhibitory substances (blis) with a strong anti-listeria activity. a modified ph-mediated adsorption-desorption purification process resulted in (after sds-page) two bands showing antimicrobial activities, where most of the activity cor ... | 2005 | 15991053 |
| microbiological survey of prepackaged pâté and ham in new zealand. | to gauge the effectiveness of pâté and ham manufacturers' management of the microbial safety and quality of their products. | 2005 | 16033505 |
| bacteriological quality of organically grown leaf lettuce in norway. | to investigate bacteriological quality in organically grown leaf lettuce, including the presence of selected pathogenic bacteria, and to obtain information about organic lettuce production, including fertilizing regimes. | 2005 | 16033519 |
| delineation of the function of a major gamma delta t cell subset during infection. | gammadelta t cells play important but poorly defined roles in pathogen-induced immune responses and in preventing chronic inflammation and pathology. a major obstacle to defining their function is establishing the degree of functional redundancy and heterogeneity among gammadelta t cells. using mice deficient in vgamma1+ t cells which are a major component of the gammadelta t cell response to microbial infection, a specific immunoregulatory role for vgamma1+ t cells in macrophage and gammadelta ... | 2005 | 16034115 |
| relative contributions of nk and cd8 t cells to ifn-gamma mediated innate immune protection against listeria monocytogenes. | during the innate immune response to listeria monocytogenes (lm), the secretion of ifn-gamma is crucial in controlling bacterial numbers. we have shown recently that cd8 t cells have the ability to rapidly secrete ifn-gamma independent of ag, in response to il-12 and il-18, during a lm infection. in the current study, we compared the relative abilities of nk and cd8 t cells to provide innate immune protection. upon transfer of either nk or memory ot-i t cells (specific for the ova protein) into ... | 2005 | 16034116 |
| cd43 is required for optimal growth inhibition of mycobacterium tuberculosis in macrophages and in mice. | we explored the role of macrophage (mphi) cd43, a transmembrane glycoprotein, in the pathogenesis of mycobacterium tuberculosis. using gene-deleted mice (cd43-/-), we assessed the association of the bacterium with distinct populations of mphi and found that cd43-/- mphi bound less m. tuberculosis than cd43+/+ mphi. increased infective doses did not abrogate this difference. however, reduced association due to the absence of cd43 could be overcome by serum components. mphi from heterozygote mice, ... | 2005 | 16034122 |
| the tlr7 agonist imiquimod enhances the anti-melanoma effects of a recombinant listeria monocytogenes vaccine. | activation of innate immune cells through tlr triggers immunomodulating events that enhance cell-mediated immunity, raising the possibility that ligands to these receptors might act as adjuvants in conjunction with t cell activating vaccines. in this report, topical imiquimod, a synthetic tlr7 agonist, significantly enhanced the protective antitumor effects of a live, recombinant listeria vaccine against murine melanoma. this tumor protective effect was not dependent on direct application to the ... | 2005 | 16034143 |
| phosphatidylinositol-dependent phospholipases c plc2 and plc3 of candida albicans are dispensable for morphogenesis and host-pathogen interaction. | phospholipases play an important role as virulence factors in human pathogens. candida albicans, the major fungal pathogen of humans, encodes phospholipases of type a, b, c and d. type b plb2 and type d pld1 phospholipases have been shown to contribute to virulence in this organism. we analyzed, in c. albicans, plc2 and plc3, two highly conserved genes coding for phosphatidylinositol-dependent phospholipases c with homology to the known virulence factor plca in the human pathogen listeria monocy ... | 2005 | 16040234 |
| listeriosis prevention knowledge among pregnant women in the usa. | listeriosis is a food-borne disease often associated with ready-to-eat foods. it usually causes mild febrile gastrointestinal illness in immunocompetent persons. in pregnant women, it may cause more severe infection and often crosses the placenta to infect the fetus, resulting in miscarriage, fetal death or neonatal morbidity. simple precautions during pregnancy can prevent listeriosis. however, many women are unaware of these precautions and listeriosis education is often omitted from prenatal ... | 2005 | 16040322 |
| independent protective effects for tumor necrosis factor and lymphotoxin alpha in the host response to listeria monocytogenes infection. | although the essential role of tumor necrosis factor (tnf) in resistance to listeria monocytogenes infection is well established, the roles of the related cytokines lymphotoxin alpha (ltalpha) and lymphotoxin beta (ltbeta) are unknown. using c57bl/6 mice in which the genes for these cytokines were disrupted, we examined the contributions of tnf, ltalpha, and ltbeta in the host response to listeria. to overcome the lack of peripheral lymph nodes in ltalpha(-/-) and ltbeta(-/-) mice, bone marrow c ... | 2005 | 16040991 |
| conditional lethality yields a new vaccine strain of listeria monocytogenes for the induction of cell-mediated immunity. | listeria monocytogenes is a gram-positive intracellular pathogen that can enter phagocytic and nonphagocytic cells and colonize their cytosols. taking advantage of this property to generate an intracellular vaccine delivery vector, we previously described a mutant strain of l. monocytogenes, deltadal deltadat, which is unable to synthesize cell wall by virtue of deletions in two genes (dal and dat) required for d-alanine synthesis. this highly attenuated strain induced long-lived protective syst ... | 2005 | 16041022 |
| 4-1bb (cd137) is required for rapid clearance of listeria monocytogenes infection. | 4-1bb (cd137), a member of the tumor necrosis factor receptor superfamily, is a t-cell-costimulatory receptor that is expressed on activated t cells, dendritic cells, and nk cells. little has been reported about its role in early host defense against bacterial infection. in this study, we report that 4-1bb-deficient (4-1bb(-/-)) mice are much more susceptible to listeria monocytogenes (intracellular bacteria) infections than wild-type mice. upon l. monocytogenes infection, 4-1bb(-/-) mice showed ... | 2005 | 16041031 |
| killed but metabolically active microbes: a new vaccine paradigm for eliciting effector t-cell responses and protective immunity. | we developed a new class of vaccines, based on killed but metabolically active (kbma) bacteria, that simultaneously takes advantage of the potency of live vaccines and the safety of killed vaccines. we removed genes required for nucleotide excision repair (uvrab), rendering microbial-based vaccines exquisitely sensitive to photochemical inactivation with psoralen and long-wavelength ultraviolet light. colony formation of the nucleotide excision repair mutants was blocked by infrequent, randomly ... | 2005 | 16041382 |
| [prevalence of listeria spp. in dairy farm and evaluation of antibiotic-resistance of isolates]. | the study was performed to estimate the prevalence and antimicrobial sensitivities of listeria spp. in raw milk, feaces end environmental samples isolated from 10 dairy in molise region. a total of 454 samples were collected, which comprised 40 raw milk, 40 animal faeces and 374 environmental samples. listeria monocytogenes was never isolated from raw milk specimens; one was isolated from faeces speciments and two were isolated from environmental samples. all isolates were resistant to two or mo ... | 2005 | 16041920 |
| use of hydrogen peroxide in combination with nisin, sodium lactate and citric acid for reducing transfer of bacterial pathogens from whole melon surfaces to fresh-cut pieces. | hydrogen peroxide (2.5%) alone or hydrogen peroxide (1%) in combination with nisin (25 microg/ml), sodium lactate (1%), and citric acid (0.5%) (hplnc) were investigated as potential sanitizers for reducing escherichia coli o157:h7 or listeria monocytogenes populations on whole cantaloupe and honeydew melons. whole cantaloupes inoculated with e. coli o157:h7 and l. monocytogenes at 5.27 and 4.07 log10 cfu/cm2, respectively, and whole honeydew melons inoculated with e. coli o157:h7 and l. monocyto ... | 2005 | 16043249 |
| cpg-activated thy1.2+ dendritic cells protect against lethal listeria monocytogenes infection. | synthetic oligodeoxynucleotides containing cpg motifs (cpg odn) activate the innate immune system by interacting with toll-like receptor 9. the resultant immune response increases host resistance to infection by a variety of pathogenic microorganisms, including listeria monocytogenes. there is a considerable interest in harnessing the immunoprotective properties of cpg odn, yet little is known of the cell phenotype(s) responsible for mediating this protection. this work demonstrates that treatme ... | 2005 | 16047338 |
| rapid purification of recombinant listeriolysin o (llo) from escherichia coli. | listeria monocytogenes is an emerging foodborne pathogen that is responsible for about 28% of the food-related deaths in the united states. it causes meningitis, septicaemia and in pregnant women, abortions and stillbirths. it secretes the toxin listeriolysin o (llo) that allows the bacteria to enter the cytoplasm of host cells, where they can replicate and cause further infection. the rapid and sensitive detection of llo in food samples is a key to monitoring and prevention of listeriosis. to f ... | 2005 | 16052337 |
| antibacterial activity of cerein 8a, a bacteriocin-like peptide produced by bacillus cereus. | the mode of action of cerein 8a, a bacteriocin produced by the soil bacterium bacillus cereus 8a, was investigated. the effect of cerein 8a was tested against listeria monocytogenes and a bactericidal effect at 400 arbitrary units (au)/ml was observed. in addition, cerein 8a was bactericidal against bacillus cereus at 200 au/ml, and inhibited the growth of escherichia coli and salmonella enteritidis. stronger inhibition of these gram-negative bacteria was achieved when the chelating agent edta w ... | 2005 | 16052461 |
| detection of pathogenic bacteria in food samples using highly-dispersed carbon particles. | there is an unmet need for detection methods that can rapidly and sensitively detect food borne pathogens. a flow through immunoassay system utilizing highly dispersed carbon particles and an amperometric technique has been developed and optimized. a sandwich immunoassay format was utilized in which pathogenic cells were captured by antibodies immobilized onto activated carbon particles, and labeled with horseradish peroxidase (hrp) conjugated antibodies. flow of the peroxidase substrates result ... | 2005 | 16076439 |
| evolution and molecular phylogeny of listeria monocytogenes isolated from human and animal listeriosis cases and foods. | to probe the evolution and phylogeny of listeria monocytogenes from defined host species and environments, l. monocytogenes isolates from human (n = 60) and animal (n = 30) listeriosis cases and food samples (n = 30) were randomly selected from a larger collection of isolates (n = 354) obtained in new york state between 1999 and 2001. partial sequencing of four housekeeping genes (gap, prs, purm, and ribc), one stress response gene (sigb), and two virulence genes (acta and inla) revealed between ... | 2005 | 16077098 |
| vaccine wakes from the dead. | 2005 | 16079877 | |
| urocortin 2 suppresses host resistance to listeria monocytogenes infection via up-regulation of interleukin-10. | previous studies have showed that corticotropin-releasing factor (crf) modulates immune response during inflammation. we investigated the effect of crf family peptides on host resistance to listeria monocytogenes infection in mice. when mice were administered ip with crf, urocortin (ucn), or ucn2 30 min prior a sublethal infection with l. monocytogenes, the numbers of bacteria in the organs of ucn2-treated mice were dramatically increased, and most of these mice succumbed. however, host resistan ... | 2005 | 16081642 |
| analysis of pcr-based methods for characterization of listeria monocytogenes strains isolated from different sources. | listeria monocytogenes strains, isolated from various sources (food, environment, and animals), were used to test different pcr-based methods to investigate their capability to define the strain origin. rapd-pcr with three primers and the sau-pcr method, in which the dna was first digested with the sau3a restriction endonuclease and then amplified with a primer designed on the restriction site, were carried out, and the profiles obtained were used to perform cluster analysis. based on the cluste ... | 2005 | 16083819 |
| control of listeria monocytogenes in model sausages by enterocin as-48. | in this work we describe the control of listeria monocytogenes cect 4032 in sausage by adding the enterocin as-48 producer strains enterococcus faecalis a-48-32 and enterococcus faecium s-32-81, and also by adding a semi-purified preparation of the bacteriocin. addition of preformed as-48 caused a significant decrease (p<0.01) in the number of viable listeria even at the lowest bacteriocin concentration tested (112 au/g). at a higher concentration (225 au/g) listeria were below the detection lev ... | 2005 | 16083820 |
| modelling inactivation of listeria monocytogenes by pulsed electric fields in media of different ph. | a study of the effect of square-wave pulsed electric fields (pef) on the inactivation of listeria monocytogenes in mcilvaine buffer of different ph (3.5-7.0) was conducted. l. monocytoges was more pef sensitive at higher electric field strengths (e) and in media of low ph. a treatment at 28 kv/cm for 400 mus that inactivated 1.5, 2.3 and 3.0 log10 cycles at ph 7.0, 6.5 and 5.0 respectively destroyed almost 6.0 log10 cycles at ph 3.5. the general shape of survival curves of l. monocytogenes pef t ... | 2005 | 16083822 |
| inhibition of foodborne bacteria by native and modified protamine: importance of electrostatic interactions. | protamine is a naturally occurring cationic antimicrobial peptide (cap) that has shown some promise for control of microorganisms in food. it was hypothesized that the antibacterial effect is partially due to protamine's electrostatic affinity to the negatively charged cell envelopes of actively growing bacteria. however, nonspecific binding of the caps to negatively charged food particles may reduce the effect in food systems. to test the hypothesis, the antibacterial efficacies of native and r ... | 2005 | 16084263 |
| role for htra in stress induction and virulence potential in listeria monocytogenes. | in silico analysis of the listeria monocytogenes genome revealed lmo0292, a gene predicted to encode a htra-like serine protease. a stable insertion mutant was constructed, revealing a requirement for htra in the listerial response to heat, acid, and penicillin stress. transcriptional analysis revealed that htra is not induced in response to heat shock but is induced in response to low ph and penicillin g stress. furthermore, htra expression was shown to be dependent upon the lisrk two-component ... | 2005 | 16085809 |
| discrimination of listeria monocytogenes contaminated commercial japanese meats. | discrimination was attempted on 14 listeria monocytogenes strains isolated from commercially available japanese pork and chicken. examination of the isolates was performed by restriction fragment length polymorphism (rflp) analysis of the chromosomal dna and amplified products and comparison of the nucleotide sequences of the amplified products. a polymorphism region containing the repeated sequences in the iap gene was amplified by the polymerase chain reaction (pcr). the genetic analyses could ... | 2005 | 16091297 |
| enterocin as-48rj: a variant of enterocin as-48 chromosomally encoded by enterococcus faecium rj16 isolated from food. | the bacteriocinogenic strain rj16 isolated from goat cheese has been identified as enterococcusfaecium by species-specific pcr, dna-rrna hybridization and rdna sequencing. purified bacteriocin from strain rj16 is a carboxypeptidase a-resistant peptide with a molecular mass (7125 da) very close to the cyclic peptide enterocin as-48. bacteriocin from strain rj16 and as-48 show identical antibacterial spectra, although the former is slightly less active on strains of listeria monocytogenes and baci ... | 2005 | 16094865 |
| membrane permeabilization, orientation, and antimicrobial mechanism of subtilosin a. | subtilosin a is an antimicrobial peptide produced by the soil bacterium bacillus subtilis that possesses bactericidal activity against a diverse range of bacteria, including listeria monocytogenes. recent structural studies have found that subtilosin a is posttranslationally modified in a unique way, placing it in a new class of bacteriocins. in this study, in order to understand the mechanism of membrane-disruption by subtilosin a, the interaction of the peptide with model phospholipid bilayers ... | 2005 | 16095584 |
| conductivity and ph dual detection of growth profile of healthy and stressed listeria monocytogenes. | in this study, growth of listeria monocytogenes in a low conductivity growth medium (lcgm) was simultaneously monitored by conductivity and ph measurements. detection times obtained from the conductivity and ph growth curves were inversely related to the initial concentration of l. monocytogenes in the medium. linear responses were found by plotting detection times obtained from both conductivity and ph growth curves as a function of initial cell concentration in the range of 10(2) to 10(7) cfu/ ... | 2005 | 16167332 |
| [do we need pathogen-free livestock for food safety?]. | the reduction of the entry of pathogens in the food chain is an important premise in improving the safety of food of animal origin. since food animals are the main reservoir for pathogens, the prevalence of pathogens in livestock is of great importance. unfortunately, classical measures to improve animal health can not exclude the presence of the main food-borne zoonotic agents (salmonella, campylobacter, listeria monocytogenes, verotoxinogene escherichia coli, toxoplasma gondii) in "clinically ... | 2005 | 16218183 |
| antibacterial and antioxidant activities of essential oils isolated from thymbra capitata l. (cav.) andoriganum vulgare l. | antilisterial activities of thymbra capitata and origanum vulgare essential oils were tested against 41 strains of listeria monocytogenes. the oil of t. capitata was mainly constituted by one component, carvacrol (79%), whereas for o. vulgare three components constituted 70% of the oil, namely, thymol (33%), gamma-terpinene (26%), and p-cymene (11%). t. capitata essential oil had a significantly higher antilisterial activity in comparison to o. vulgare oil and chloramphenicol. no significant dif ... | 2005 | 16218659 |
| outbreak of clinical listeriosis in sheep: evaluation from possible contamination routes from feed to raw produce and humans. | we report the results of clinical and microbiological investigations on listeria monocytogenes infections in a flock of 55 sheep and describe the implications for the safety of the raw milk and raw-milk cheeses produced in the on-farm dairy. the outbreak was caused by feeding grass silage, which was contaminated with 5 log10 cfu l. monocytogenes/g. clinically, although having been fed from the same batch of silage, abortive (nine ewes), encephalitic (one ewe) and septicaemic (four ewes) forms of ... | 2005 | 16219091 |
| [meningitis caused by listeria monocytogenes in a patient with myasthenia gravis being treated with tacrolimus]. | 2005 | 15795876 | |
| differential cytosolic delivery and presentation of antigen by listeriolysin o-liposomes to macrophages and dendritic cells. | delivery of antigenic protein to the cytosol of antigen-presenting cells (apcs), such as macrophages (mphi) and dendritic cells (dcs), is required for an efficient cd8 t-cell-mediated immune response. we have previously shown that co-encapsulation of antigenic protein inside ph-sensitive liposomes with listeriolysin o (llo), a pore-forming protein of listeria monocytogenes, generates efficient major histocompatibility complex class i (mhc i)-restricted immune responses both in vitro and in vivo. ... | 2005 | 15804180 |
| super paramagnetic clustering of protein sequences. | detection of sequence homologues represents a challenging task that is important for the discovery of protein families and the reliable application of automatic annotation methods. the presence of domains in protein families of diverse function, inhomogeneity and different sizes of protein families create considerable difficulties for the application of published clustering methods. | 2005 | 15804359 |
| the metalloprotease of listeria monocytogenes controls cell wall translocation of the broad-range phospholipase c. | listeria monocytogenes is a gram-positive bacterial pathogen that multiplies in the cytosol of host cells and spreads directly from cell to cell. during cell-to-cell spread, bacteria become temporarily confined to secondary vacuoles. the broad-range phospholipase c (pc-plc) of l. monocytogenes contributes to bacterial escape from secondary vacuoles. pc-plc requires cleavage of an n-terminal propeptide for activation, and mpl, a metalloprotease of listeria, is involved in the proteolytic activati ... | 2005 | 15805506 |
| enhanced synthesis of internalin a in aro mutants of listeria monocytogenes indicates posttranscriptional control of the inlab mrna. | listeria monocytogenes mutants with deletions in aroa, arob, or aroe exhibited strong posttranscriptional upregulation of internalin a (inla) and inlb synthesis, which resulted in a more-than-10-fold increase in inla-mediated internalization by epithelial caco-2 cells and a 4-fold increase in inlb-mediated internalization by microvascular endothelial cells (human brain microvascular endothelial cells) compared to the wild-type strain. the increase in inla and inlb production was not due to enhan ... | 2005 | 15805530 |
| [lymphocytis meningitis: listeria monocytogenes is a potential risk in a immunocompetent child]. | meningoencephalitis due to listeria monocytogenes is a rare and serious form of brainstem infection in childhood. observation: we report the case of a 7 year-old girl presenting lymphocytic meningitis with a high crp level. parenteral antibiotics combining ceftriaxone and vancomycine led initially to clinical improvement. ten days later, secondary brainstem inflammation with hydrocephalus appeared and led to the detection of l. monocytogenes during external ventricular bypass. conclusion: this o ... | 2005 | 16185855 |
| effectiveness of steam pasteurization in controlling microbiological hazards of cull cow carcasses in a commercial plant. | the purpose of the study, carried out in a beef processing plant, was to evaluate the effectiveness of a new prototype for steam pasteurization treatment in controlling microbiological hazards. samples were taken by swabbing randomly selected sites before and after pasteurization and again after chilling to obtain total aerobic counts (tac), total coliform counts (tcc), and generic escherichia coli counts (ecc) on petrifilm plates and to determine the prevalence of salmonella spp., listeria mono ... | 2005 | 16187550 |
| bacteriophage p100 for control of listeria monocytogenes in foods: genome sequence, bioinformatic analyses, oral toxicity study, and application. | listeria monocytogenes is an opportunistic foodborne pathogen responsible for listeriosis, a frequently fatal infection. this investigation represents a comprehensive approach to characterize and evaluate the broad host range, strictly virulent phage p100, which can infect and kill a majority of listeria monocytogenes strains. first, the complete nucleotide sequence (131,384 basepairs) of the genome of p100 was determined, predicted to encode 174 gene products and 18 trnas. bioinformatic analyse ... | 2005 | 16188359 |
| potential antimicrobial effects of human lactoferrin against oral infection with listeria monocytogenes in mice. | listeria monocytogenes is a food-borne pathogen that causes serious listeriosis in humans. antimicrobial effects of human lactoferrin (hlf) against l. monocytogenes have been clearly demonstrated in in vitro studies. however, in vivo studies have not been reported yet. this study investigated whether the oral administration of hlf could inhibit oral infection of listeria in balb/c mice. the mics for several strains of l. monocytogenes were determined, and the most sensitive strain was used for t ... | 2005 | 16192436 |
| neuropathological findings in 9 cases of listeria monocytogenes brain stem encephalitis. | brain stem encephalitis is a particular manifestation of infection with the bacterium listeria monocytogenes. here, we present the neuropathological findings in 9 such cases. in the brain stem, the inflammatory infiltrates were located predominantly within nuclei and tracts of cranial nerves innervating the oropharynx. these findings support the hypothesis that the food-borne bacterium listeria monocytogenes invades the brain stem along cranial nerves. | 2005 | 16196384 |
| symmetry-breaking motility. | locomotion of bacteria by actin polymerization and in vitro motion of spherical beads coated with a protein catalyzing polymerization are examples of active motility. starting from a simple model of forces locally normal to the surface of a bead, we construct a phenomenological equation for its motion. the singularities at a continuous transition between moving and stationary beads are shown to be related to the symmetries of its shape. universal features of the phase behavior are calculated ana ... | 2005 | 16197183 |
| the food safety management system. | a comprehensive food safety strategy involves establishing risk management goals, food safety objectives and, for production systems, performance objectives and performance criteria. the working instructions for each step of the process should be validated for their effect before integration within a specific haccp plan. the importance of realistic inactivation models to predict the hygienic equivalence of food processing operations is discussed. | 2005 | 16244936 |
| molecular characterization of listeria monocytogenes strains associated with outbreaks of listeriosis in humans and ruminants and food products by serotyping and automated ribotyping. | 2005 | 16244967 | |
| antimicrobial effect of thai spices against listeria monocytogenes and salmonella typhimurium dt104. | the objective of this study was to determine the potential antimicrobial activity of extracts and essential oils of spices from thailand against foodborne pathogenic bacteria. the antimicrobial efficacy of ginger (zingiber officinale), fingerroot (boesenbergia pandurata), and turmeric (curcuma longa) was evaluated against five strains of listeria monocytogenes and four strains of salmonella enterica ssp. enterica serovar typhimurium dt104. antimicrobial activity was investigated in microbiologic ... | 2005 | 16245707 |
| evaluation of small-scale hot-water postpackaging pasteurization treatments for destruction of listeria monocytogenes on ready-to-eat beef snack sticks and natural-casing wieners. | this study was conducted to evaluate small-scale hot-water postpackaging pasteurization (ppp) as a postlethality (post-cooking) treatment for listeria monocytogenes on ready-to-eat beef snack sticks and natural-casing wieners. using a commercially available plastic packaging film specifically designed for ppp applications and 2.8 liters of boiling water (100 degrees c) in a sauce pan on a hot plate, an average reduction in l. monocytogenes numbers of > or = 2 log units was obtained using heating ... | 2005 | 16245708 |
| antilisterial activity of a carnobacterium piscicola isolated from brazilian smoked fish (surubim [pseudoplatystoma sp.]) and its activity against a persistent strain of listeria monocytogenes isolated from surubim. | data on the prevalence and growth of listeria monocytogenes in lightly preserved fish products from subtropical and tropical regions are very scarce. our research describes l. monocytogenes that was detected in 5% of the packages of cold-smoked surubim, a native brazilian freshwater fish that we analyzed, and shows that the strains isolated were of the same random amplified polymorphic dna subtype as the strains that were isolated from the same factory 4 years earlier. a bacteriocinogenic strain ... | 2005 | 16245709 |
| stability of enterocin as-48 in fruit and vegetable juices. | enterocin as-48 is a candidate bacteriocin for food biopreservation. before addressing application of as-48 to vegetable-based foods, the interaction between as-48 and vegetable food components and the stability of as-48 were studied. enterocin as-48 had variable interactions with fruit and vegetable juices, with complete, partial, or negligible loss of activity. for some juices, loss of activity was ameliorated by increasing the bacteriocin concentration, diluting the juice, or applying a heat ... | 2005 | 16245711 |
| efficacy of uv light for the reduction of listeria monocytogenes in goat's milk. | certain types of goat's cheeses are produced using unpasteurized milk, which increases the food safety concerns for these types of products. popularity and consumption of goat's milk products have increased, and the niche market includes gourmet goat's cheeses. the u.s. code of federal regulations and the pasteurized milk ordinance both address the possibility for processing alternatives to heat treatment, and the use of uv light treatment may be a viable alternative that still ensures the safet ... | 2005 | 16245732 |
| [effect of high hydrostatic pressure on microbial physiological characteristics]. | physiological characterizations of listeria monocytogenes nctc 11994 and escherichia coli atcc 80739 have deeply changed by high hydrostatic pressure. the results showed that counts of both microbial strains decreased 7 log cfu at 400mpa, 10 min. pressure treatments also resulted in change of intracellular ph value, lowed membrane potential, have internal potassium filtered out, and decreased atp concentration. | 2005 | 16245862 |
| identification of substrates of the listeria monocytogenes sortases a and b by a non-gel proteomic analysis. | sortases are enzymes that anchor surface proteins to the cell wall of gram-positive bacteria by cleaving a sorting motif located in the c-terminus of the protein substrate. the best-characterized motif is lpxtg, which is cleaved between the t and g residues. in this study, a non-gel proteomic approach was used to identify surface proteins recognized by the two sortases of listeria monocytogenes, srta and srtb. material containing peptidoglycan and strongly associated proteins was purified from s ... | 2005 | 16247833 |
| listeria-based vaccines for cancer treatment. | listeria monocytogenes is a facultative intracellular bacterium that enters professional antigen-presenting cells by active phagocytosis. as a live bacterium, it induces antigen-presenting cell maturation and strong innate immunity which may assist in the immune response to poorly immunogenic antigens, such as tumor-associated antigens. listeria produces virulence factors that allow it to escape from the phagolysosome and colonize the cytosol of the host cell. it is thus a potent vaccine vector ... | 2005 | 16248280 |
| comparison of genomic structures in the serovar 1/2a listeria monocytogenes isolated from meats and listeriosis patients in japan. | foodborne disease by listeria monocytogenes, serovar 1/2a has recently been reported in many countries. although contamination by this bacteria is also known to be gradually spreading among the marketed foods of japan, there is little information on relation between listeriosis and food contamination. in the present study, the characteristics of the genomic structures of serovar 1/2a were compared among the isolates from marketed meats and listeriosis patients. several isolates from meats purcha ... | 2005 | 16249623 |
| analysis of the molecular evolution of listeria monocytogenes isolated from japanese meats and environment. | food contaminated by listeria monocytogenes is a problem on a worldwide level because it is a serious food-borne pathogen. although 3 evolutionary divisions have been reported for l. monocytogenes, the evolution of japanese isolates has not yet been clarified. thus, in order to determine the lineage of these japanese isolates, we classified and conducted phylogenetic analysis of 407 bp (position 1116-1522) of the iap gene derived from 88 isolates from japanese listeriosis patients, foods and env ... | 2005 | 16249630 |
| adhesion controls bacterial actin polymerization-based movement. | as part of its infectious life cycle, the bacterial pathogen listeria monocytogenes propels itself through the host-cell cytoplasm by triggering the polymerization of host-cell actin near the bacterial surface, harnessing the activity of several cytoskeletal proteins used during actin-based cell crawling. to distinguish among several classes of biophysical models of actin-based bacterial movement, we used a high-throughput tracking technique to record the movement of many individual bacteria dur ... | 2005 | 16251274 |
| roles of group iia phospholipase a2 and complement in killing of bacteria by acute phase serum. | the complement system is regarded as an important component of the innate defence system against invading bacteria. however, synergistic actions between the complement and the other components of innate immunity are incompletely known. human group iia phospholipase a(2) (hgiia pla(2)) is an effective antibacterial enzyme in serum of patients with severe bacterial infections. our aim was to investigate the significance of complement and hgiia pla(2) in acute phase serum. serum samples were collec ... | 2005 | 16253130 |
| arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function. | rna interference silencing of up to 90% of arp3 protein expression, a major subunit of the arp2/3 complex, proportionately decreases the intracellular motility of listeria monocytogenes and actin nucleation activity ascribable to the arp2/3 complex in mouse embryonic fibroblasts. however, the arp2/3-deficient cells exhibit unimpaired lamellipodial actin network structure, translational locomotion, spreading, actin assembly, and ruffling responses. in addition, arp3-silenced cells expressing neur ... | 2005 | 16254049 |
| real-time polymerase chain reaction for detecting bacterial dna directly from blood of neonates being evaluated for sepsis. | speed is of the essence when evaluating an infant with symptoms consistent with sepsis. because of the high morbidity and mortality associated with neonatal sepsis, infants receive multiple, broad-spectrum antibiotics before receiving finalized blood culture results. incorporating an additional, reliable, yet rapid assay to detect bacteria directly from blood would facilitate timely diagnosis and appropriate care. to this end, we designed a real-time polymerase chain reaction (pcr) assay targeti ... | 2005 | 16258155 |
| magnetic nanoparticle-antibody conjugates for the separation of escherichia coli o157:h7 in ground beef. | the immunomagnetic separation with magnetic nanoparticle-antibody conjugates (mncs) was investigated and evaluated for the detection of escherichia coli o157:h7 in ground beef samples. mncs were prepared by immobilizing biotin-labeled polyclonal goat anti-e. coli antibodies onto streptavidin-coated magnetic nanoparticles. for bacterial separation, mncs were mixed with inoculated ground beef samples, then nanoparticle-antibody-e. coli o157:h7 complexes were separated from food matrix with a magne ... | 2005 | 16161677 |
| antimicrobial activity of cetylpyridinium chloride against listeria monocytogenes on frankfurters and subsequent effect on quality attributest. | frankfurters inoculated with listeria monocytogenes were treated with 1% cetylpyridinium chloride (cpc) or with 1% cpc followed by a water rinse at various combinations of spray temperatures (25, 40, and 55 degrees c), spray pressures (20, 25, and 35 psi), and times of exposure (30, 40, and 60 s). no significant differences (p > 0.05) were observed in the reductions achieved by 1% cpc + water wash and those achieved with 1% cpc treatment alone. l. monocytogenes populations were reduced by ca. 1. ... | 2005 | 16161680 |
| a field study of the microbiological quality of fresh produce. | the centers for disease control and prevention has reported that foodborne disease outbreaks associated with fruits and vegetables increased during the past decade. this study was conducted to characterize the routes of microbial contamination in produce and to identify areas of potential contamination from production through postharvest handling. we report here the levels of bacterial indicator organisms and the prevalence of selected pathogens in produce samples collected from the southern uni ... | 2005 | 16161682 |
| occurrence of campylobacter and listeria monocytogenes in a poultry processing plant. | the occurrence of campylobacter and listeria monocytogenes was studied in 645 samples from surfaces, water, and poultry products (chicken carcasses, chicken parts, viscera, and spoils) in a poultry processing plant in southern brazil. the automated mini-vidas system was used to detect the presence of campylobacter and l. monocytogenes on the samples. the positive samples were confirmed by conventional methods. campylobacter and l. monocytogenes were found in 16.6 and 35.6% of the analyzed sample ... | 2005 | 16161691 |
| multiplex pcr assay simplifies serotyping and sequence typing of listeria monocytogenes associated with human outbreaks. | listeria monocytogenes serotypes 1/2a and 4b are responsible for the majority of cases of human listeriosis worldwide. in this study, a multiplex pcr assay was developed to allow rapid identification and easily interpretable differentiation of serotypes 1/2a and 4b from other serotypes of l. monocytogenes by simultaneously targeting two virulence genes (inlb and inlc) and two serotype-specific genes (orf2372 and imo0171). a subsequent gel extraction and sequence typing analysis of the highly pol ... | 2005 | 16161692 |
| achieving continuous improvement in reductions in foodborne listeriosis--a risk-based approach. | listeria monocytogenes is a foodborne pathogen that can cause listeriosis, a severe disease that can lead to septicemia, meningitis, and spontaneous abortion. ongoing efforts are needed to further reduce the incidence of listeriosis, due to its high mortality rate. the focus of this report is the use of a risk-based approach to identify strategies that will have the greatest impact on reducing foodborne listeriosis. a continuum of risk for listeriosis is observed in the human population, ranging ... | 2005 | 16161698 |
| rat cytomegalovirus and listeria monocytogenes infection enhance chronic rejection after allogenic rat lung transplantation. | the role of infection in the pathomechanism of obliterative bronchiolitis (ob) after human lung transplantation is controversial. in a rat lung transplantation model, we analyzed the effect of viral [rat cytomegalovirus (rcmv)] and bacterial infection [listeria monocytogenes (lm)] on the development of chronic allograft rejection. fisher rats underwent single left lung transplantation with allografts from lewis rats. postoperatively, animals were infected with either rcmv or lm, or served as non ... | 2005 | 16162104 |
| effect of laser and environmental parameters on reducing microbial contamination of stainless steel surfaces with nd:yag laser irradiation. | the effect of laser (pulse repetition frequency, pulse energy and exposure time) and environmental parameters (ph, nacl concentration and wet or dry samples) of nd:yag laser decontamination of stainless steel inoculated with escherichia coli, staphylococcus aureus and listeria monocytogenes was investigated. | 2005 | 16162246 |
| antimicrobial susceptibility of nisin resistant listeria monocytogenes of dairy origin. | the antibiotic susceptibility of wild listeria monocytogenes strains and their corresponding nisin resistant variants was assessed. the resistant strains were more sensitive to most of the tested antibiotics than their wild-type counterparts. a slight increase in mic was observed for a few antibiotics including the membrane disturbing polymixin b. cross-resistance was detected with two synthetic antimicrobial peptides. a lower c15/c17 ratio in the membrane fatty acid composition of the nisin res ... | 2005 | 16165322 |
| genomic fingerprinting of bacteriocin-producer strains of staphylococcus aureus. | among 363 strains of staphylococcus aureus, 21 were shown to produce bacteriocins (bac), antimicrobial peptides with potential biotechnological applications. this collection includes strains which are either isolated from food, patients and healthy cattle, or are involved in subclinical bovine mastitis. from these 21 strains, 17 were shown to carry closely-related 8.0-kb bac plasmids encoding bacteriocins either identical to or similar to aureocin a70, a bacteriocin able to inhibit strains of li ... | 2005 | 16171981 |
| high pressure processing for food safety. | food preservation using high pressure is a promising technique in food industry as it offers numerous opportunities for developing new foods with extended shelf-life, high nutritional value and excellent organoleptic characteristics. high pressure is an alternative to thermal processing. the resistance of microorganisms to pressure varies considerably depending on the pressure range applied, temperature and treatment duration, and type of microorganism. generally, gram-positive bacteria are more ... | 2005 | 16175246 |
| expression of t-bet by cd4 t cells is essential for resistance to salmonella infection. | despite the recognized role of the t-bet transcription factor in the differentiation of th1 cells, t-bet-deficient mice can develop small numbers of ifn-gamma-producing cd4 t cells. although these are not sufficient to allow normal handling of some pathogens, t-bet-deficient mice do resolve infection with the intracellular pathogen listeria monocytogenes. in contrast, we report that expression of t-bet is required for resistance to salmonella infection. t-bet-deficient mice succumbed to infectio ... | 2005 | 16177105 |
| a novel role of cd30/cd30 ligand signaling in the generation of long-lived memory cd8+ t cells. | memory cd8+ t cells can be divided into two subsets, central memory (t(cm)) and effector memory (t(em)) cd8+ t cells. we found that cd30, a member of the tnfr-associated factor (traf)-linked tnfr superfamily, signaling is involved in differentiation of long-lived cd8+ t(cm) cells following listeria monocytogenes infection. although cd8+ t(em) cells transiently accumulated in the nonlymphoid tissues of cd30 ligand (cd153-/-) mice after infection, long-lived memory cd8+ t(cm) cells were poorly gen ... | 2005 | 16177108 |
| th1 cytokines are essential for placental immunity to listeria monocytogenes. | the fetal allograft poses an immunological challenge: how is it protected while immunity to pathogens, particularly those that replicate in the placenta, is maintained? several theories have been proposed to explain this fetal protection, including a pregnancy-based bias towards a th2 rather than th1 cytokine profile in order to avoid generating cytotoxic t cells that could threaten the fetus. listeria monocytogenes preferentially replicates in the placenta and systemically requires a th1 respon ... | 2005 | 16177303 |
| characterization of listeria monocytogenes expressing anthrolysin o and phosphatidylinositol-specific phospholipase c from bacillus anthracis. | two virulence factors of listeria monocytogenes, listeriolysin o (llo) and phosphatidylinositol-specific phospholipase c (pi-plc), mediate escape of this pathogen from the phagocytic vacuole of macrophages, thereby allowing the bacterium access to the host cell cytosol for growth and spread to neighboring cells. we characterized their orthologs from bacillus anthracis by expressing them in l. monocytogenes and characterizing their contribution to bacterial intracellular growth and cell-to-cell s ... | 2005 | 16177340 |
| lpxtg protein inlj, a newly identified internalin involved in listeria monocytogenes virulence. | listeria monocytogenes expresses surface proteins covalently anchored to the peptidoglycan by sortase enzymes. inactivation of srta attenuates listeria virulence in mice (h. bierne, s. k. mazmanian, m. trost, m. g. pucciarelli, g. liu, p. dehoux, l. jansch, f. garcia-del portillo, o. schneewind, and p. cossart, mol. microbiol. 43:869-881, 2002). we show here that an srta mutant is more attenuated than an internalin mutant in orally infected guinea pigs and transgenic mice expressing human e-cadh ... | 2005 | 16177371 |