Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| one-year (2003) nationwide pork carcass microbiological baseline data survey in taiwan. | from january through december 2003, swab samples from 1,650 pork carcasses were collected from 39 slaughter plants in taiwan. these samples were analyzed for the prevalence of indicator microorganisms and specific pathogens. viable aerobic bacteria, total coliforms, and escherichia coli were recovered from 100, 95.3, and 87.5% of these carcasses, respectively. of those carcasses that harbored bacteria, the mean aerobic plate, total coliform, and escherichia coli counts were 4.0, 0.6, and 0.1 log ... | 2005 | 15771166 |
| elimination of listeria monocytogenes biofilms by ozone, chlorine, and hydrogen peroxide. | this study evaluated the efficacy of ozone, chlorine, and hydrogen peroxide to destroy listeria monocytogenes planktonic cells and biofilms of two test strains, scott a and 10403s. l. monocytogenes was sensitive to ozone (o3), chlorine, and hydrogen peroxide (h2o2). planktonic cells of strain scott a were completely destroyed by exposure to 0.25 ppm o3 (8.29-log reduction, cfu per milliliter). ozone's destruction of scott a increased when the concentration was increased, with complete eliminatio ... | 2005 | 15771172 |
| inhibitory effects of organic acid salts for control of listeria monocytogenes on frankfurters. | sodium diacetate (sd), sodium diacetate plus potassium benzoate (sd-pb), and sodium lactate plus sodium diacetate plus potassium benzoate (sl-sd-pb) were selected for initial effectiveness against listeria monocytogenes on frankfurters. treatments were evaluated at -2.2, 1.1, 4.4, 10.0, and 12.8 degrees c for up to 90 days. the compounds were applied as 3 or 6% (total concentration) dipping solutions for surface treatment of the frankfurters. the treated frankfurters were inoculated with a five- ... | 2005 | 15771173 |
| eradicating listeria monocytogenes from fully cooked franks by using an integrated pasteurization-packaging system. | surface pasteurization by applying steam or hot water before or after packaging of processed foods may be used to eliminate pathogens such as listeria monocytogenes from ready-to-eat meat and poultry products. surface pasteurization treatment with a mixture of pressurized steam and hot water was integrated into a continuous vacuum-packaging system to reduce l. monocytogenes from fully cooked franks. the franks (2.54 cm diameter by 15.24 cm length) were surface inoculated to contain up to 6 log c ... | 2005 | 15771174 |
| destruction of listeria monocytogenes in sturgeon (acipenser transmontanus) caviar by a combination of nisin with chemical antimicrobials or moderate heat. | the objective of this study was to investigate the effect of nisin in combination with heat or antimicrobial chemical treatments (such as lactic acid, chlorous acid, and sodium hypochlorite) on the inhibition of listeria monocytogenes and total mesophiles in sturgeon (acipenser transmontanus) caviar. the effects of nisin (250, 500, 750, and 1,000 iu/ml), lactic acid (1, 2, and 3%), chlorous acid (134 and 268 ppm), sodium hypochlorite (150 and 300 ppm), and heat at 60 degrees c for 3 min were eva ... | 2005 | 15771175 |
| multiplex pcr for simultaneous detection of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7 in meat samples. | a multiplex pcr method was developed for simultaneous detection of salmonella spp., listeria monocytogenes, and escherichia coli o157:h7 in meat samples. dna detection sensitivity for this method was 10(3) cfu/ml for each pathogen. when this protocol was used for the detection of each of the above pathogenic bacteria in spiked pork samples, 1 cell per 25 g of inoculated sample could be detected within 30 h. in the samples of naturally contaminated meat, salmonella spp., l. monocytogenes, and e. ... | 2005 | 15771181 |
| effects of suspension in emulsified wiener or incubation in wiener packages on the virulence of listeria monocytogenes scott a in intragastrically inoculated a/j mice. | several outbreaks of listeriosis have been associated with contamination of wieners and other ready-to-eat meat products. in this study, we addressed the question of whether emulsification in, or growth on, wieners triggers a response in the listerial cells that makes them more virulent or protects them against the harsh environment of the gastrointestinal tract in mice. our results indicate that listeria monocytogenes scott a grows poorly, if at all, in one brand of commercially prepared wiener ... | 2005 | 15771188 |
| enhanced plasmid dna delivery using anionic lpdii by listeriolysin o incorporation. | a major obstacle to achieving effective dna-based therapeutics is efficient delivery of the dna to its site of action in the cell. upon internalization by endocytosis, the endosomal membrane represents a critical physical barrier preventing access of dna to the cell cytosol. in order to overcome the membrane barrier and facilitate cytosolic entry, the endosomolytic bacterial protein listeriolysin o (llo) is a potentially promising agent. | 2005 | 15776501 |
| yersinia outer protein p inhibits cd8 t cell priming in the mouse infection model. | pathogenic yersiniae translocate a mixture of effector proteins called yersinia outer proteins (yops) into the cytosol of eukaryotic cells by their type iii secretion system. yopp is one of the best characterized of these effector proteins and known to inhibit the proinflammatory response of the host by interfering with nf-kappab signal transduction and inducing apoptosis of macrophages. the effects of yopp on the immune response were studied by a yersinia ag-independent approach using bacteria ... | 2005 | 15778387 |
| protective cd8 t cell immunity triggered by cpg-protein conjugates competes with the efficacy of live vaccines. | in contrast to infectious (live) vaccines are those based on subunit ag that are notoriously poor in eliciting protective cd8 t cell responses, presumably because subunit ags become insufficiently cross-presented by dendritic cells (dcs) and because the latter need to be activated to acquire competence for cross-priming. in this study, we show that cpg-ag complexes overcome these limitations. ova covalently linked to cpg-dna (cpg-ova complex), once it is efficiently internalized by dcs via dna r ... | 2005 | 15778402 |
| isolation and pcr amplification of a species-specific oxidoreductase-coding gene region in listeria grayi. | listeria grayi is a nonpathogenic gram-positive bacterium that demonstrates considerable similarities to other members in the genus listeria, including the foodborne human pathogen listeria monocytogenes and the animal pathogen listeria ivanovii. a rapid diagnostic test to identify and diagnose listeriosis would be valuable, especially in cases where the presence of l. grayi may complicate diagnosis. this test would be based on a unique gene present in l. grayi. in this study, after comparative ... | 2005 | 15782240 |
| pleiotropic enhancement of bacterial pathogenesis resulting from the constitutive activation of the listeria monocytogenes regulatory factor prfa. | listeria monocytogenes is a facultative intracellular bacterial pathogen that causes serious disease in immunocompromised individuals, pregnant women, and neonates. bacterial virulence is mediated by the expression of specific gene products that facilitate entry into host cells and enable bacterial replication; the majority of these gene products are regulated by a transcriptional activator known as prfa. l. monocytogenes strains containing prfa e77k or prfa g155s mutations exhibit increased exp ... | 2005 | 15784531 |
| redundant roles for met docking site tyrosines and the gab1 pleckstrin homology domain in inlb-mediated entry of listeria monocytogenes. | the bacterial pathogen listeria monocytogenes causes food-borne illnesses leading to gastroenteritis, meningitis, or abortion. listeria induces its internalization into some mammalian cells through interaction of the bacterial surface protein inlb with host met receptor tyrosine kinase. binding of inlb leads to phosphorylation of met and the adapter gab1 and to activation of host phosphoinositide (pi) 3-kinase. the mammalian ligand of met, hepatocyte growth factor, promotes cell motility and mor ... | 2005 | 15784547 |
| mouse paneth cell secretory responses to cell surface glycolipids of virulent and attenuated pathogenic bacteria. | mouse paneth cells respond to bacteria and bacterial cell surface antigens by discharging secretory granules into the lumen of small intestinal crypts (t. ayabe et al., nat. immunol. 1:113-118, 2000). to investigate mechanisms regulating these responses, purified surface glycolipid molecules with known acyl chain modifications and attenuated properties were tested for the ability to stimulate paneth cell secretion. the antigens included lipopolysaccharide (lps) from wild-type and msbb-null esche ... | 2005 | 15784576 |
| development of a sandwich elisa for the detection of listeria spp. using specific flagella antibodies. | five monoclonal antibodies (mabs) and chicken immunoglobulin (igy) were developed by immunizing with flagella purified from listeria monocytogenes 4b and the five mabs have been confirmed to be specific against three different epitopes of flagellin. the antibodies showed specific reaction to listeria genus and no cross-reactivity with other bacteria tested in this experiment including e.coli o157:h7 and salmonella enteritidis. sandwich enzyme-linked immunosorbent assays (elisa) using the mabs an ... | 2005 | 15785122 |
| listeria monocytogenes protein p60 affects hemolytic activity and uptake of bacteria by macrophages. | bacillus subtilis strains expressing listeriolysin o (llo) and simultaneously llo and p60 protein were constructed. the effect of p60 protein on hemolytic activity and on the invasion of professional phagocytes was demonstrated in the absence of other virulence factors of l. monocytogenes. the hemolytic activity of llo in the presence of p60 protein decreased which indicates that p60 promoted adhesion and subsequent invasion of professional phagocytes. | 2005 | 16681150 |
| [antibiotic susceptibility of listeria monocytogenes strains isolated in the primor'e region]. | antibiotic susceptibility of the listeria monocytogenes isolates from biotic and abiotic objects of the environment in the primor'e region was estimated. 100% of the isolates proved to be susceptible to benzylpenicillin, ampicillin, carbenicillin, gentamicin, doxycycline, tetracycline, vancomycin, cefazolin and rifampicin. 96, 92 and 84% of the isolates were susceptible to roxithromycin, clarithromycin and ofloxacin respectively. no significant differences were detected in the susceptibility of ... | 2005 | 16768211 |
| wasp family proteins act between cytoskeleton and cellular signaling pathways. | this review considers the proteins of the wasp (wiskott-aldrich syndrome protein) family and their role in the regulation of actin-based motility. it contains detailed classification of the wasp family proteins and data on their subcellular localization. impairments of expression of the wasp family proteins cause certain cell pathologies. the review also deals with domain organization of these proteins and proteins interacting with various domains of the wasp proteins. special attention is given ... | 2005 | 16417451 |
| prevalence of listeria monocytogenes in 13 dried sausage processing plants and their products. | the aims of the present study were: (i) to investigate the occurrence of listeria monocytogenes in dried sausage processing plants on surfaces before and during processing, (ii) to study the contamination in meat and sausages at different stages of maturation, (iii) to assess the distribution of l. monocytogenes in the different plants and products studied. thirteen dried sausage processing plants were sampled at two different times of the working day. the studies were repeated twice to evaluate ... | 2005 | 15925005 |
| cytotoxic activity of murine resident peritoneal cells against listeria monocytogenes-infected hepatocytes in vitro. | sterile immunity to the gram-positive facultative intracellular bacterium listeria monocytogenes critically depends on cytotoxic cd8+ t lymphocytes. however, the cytotoxic cell population able to kill infected cells before specific t cells are generated is not well characterised. based on histological observations and the use of monoclonal antibodies abrogating the cd11b/cd18-dependent cellular influx into infected organs as well as granulocyte-depleting antibodies, some authors favour pmns as b ... | 2005 | 15925534 |
| survival of listeria monocytogenes on fresh and frozen strawberries. | cut or intact surfaces of fresh strawberries were spot inoculated with a five-strain cocktail of nalidixic-acid resistant listeria monocytogenes (10(6) (low inoculum) and 10(8) (high inoculum) cfu per three-berry sample). inoculated strawberries were dried for 1 h at 24 degrees c and were stored in loosely closed containers at 4 or 24 degrees c. an initial population reduction of approximately 0.6 and 1.2 log cycles, high and low inoculum, respectively, was observed on intact but not cut berries ... | 2005 | 15925709 |
| cell division theory and individual-based modeling of microbial lag: part i. the theory of cell division. | this series of two papers deals with the theory of cell division and its implementation in an individual-based modeling framework. in this first part, the theory of cell division is studied on an individual-based level in order to learn more about the mechanistic principles behind microbial lag phenomena. while some important literature on cell division theory dates from 30 to 40 years ago, until now it has hardly been introduced in the field of predictive microbiology. yet, it provides a large ... | 2005 | 15925713 |
| synergism between active listeriolysin o and dimethyldioctadecylammonium bromide to activate cd8(+) t cells. | purified recombinant listeriolysin o (llo) was assessed for its ability to induce t cell responses in mice. intraperitoneal immunisation with llo, as a fusion with glutathione-s-transferase (gst), induced the production of llo-specific cd8(+) t cells, but not llo-specific cd4(+) t cells. the generation of this response could be blocked by pre-treatment with cholesterol, indicating a requirement for llo pore formation. an increase in the llo-specific response of both cd8(+) and cd4(+) t cells cou ... | 2005 | 15927321 |
| the marked increase of listeria monocytogenes isolation from contents of swine cecum. | the actual prevalence of listeria monocytogenes from contents of swine cecum was investigated. the efficiency of listeria enrichment broth (leb) for isolation was examined by the recovery of artificially inoculated l. monocytogenes in contents of swine cecum. the numbers of organisms did not increase after 48 h incubation, but increased when the rapid decrease in ph of the leb was adjusted. between 1991 and 1993, 250 contents of swine cecum were examined for the prevalence of l. monocytogenes us ... | 2005 | 15932770 |
| presence of gadd1 glutamate decarboxylase in selected listeria monocytogenes strains is associated with an ability to grow at low ph. | the glutamate decarboxylase (gad) system is critical to the survival of listeria monocytogenes lo28 at low-ph stress (<ph 4.0). the gad system classically involves two proteins, a glutamate decarboxylase enzyme coupled to a glutamate/gamma-aminobutyrate antiporter, which results in the consumption of an intracellular proton for each glutamate entering the system. uniquely among prokaryotes, some strains of l. monocytogenes, including strain lo28, possess genes encoding three decarboxylases (gadd ... | 2005 | 15932974 |
| influence of stress on individual lag time distributions of listeria monocytogenes. | the effects of nine common food industry stresses on the times to the turbidity (t(d)) distribution of listeria monocytogenes were determined. it was established that the main source of the variability of t(d) for stressed cells was the variability of individual lag times. the distributions of t(d) revealed that there was a noticeable difference in response to the stresses encountered by the l. monocytogenes cells. the applied stresses led to significant changes of the shape, the mean, and the v ... | 2005 | 15932988 |
| amplified intergenic locus polymorphism as a basis for bacterial typing of listeria spp. and escherichia coli. | dna-based methods are increasingly important for bacterial typing. the high number of polymorphic sites present among closely related bacterial genomes is the basis for the presented method. the method identifies multilocus genomic polymorphisms in intergenic regions termed ailp (amplified intergenic locus polymorphism). for each locus, a pair of unique pcr primers was designed to amplify an intergenic sequence from one open reading frame (orf) to the adjacent orf. presence, absence, and size va ... | 2005 | 15933014 |
| bacteriocin from honeybee beebread enterococcus avium, active against listeria monocytogenes. | enterococcus avium isolated from apis mellifera beebread produces a thermoresistant bacteriocin with a strain-dependent inhibitory effect on listeria and without effect on gram-negative bacteria. the bacteriocin appeared to be a polypeptide of about 6 kda. genetic analyses revealed no extrachromosomal material in e. avium. | 2005 | 15933045 |
| cd70+ antigen-presenting cells control the proliferation and differentiation of t cells in the intestinal mucosa. | one unresolved issue in gut immunity is how mucosal t lymphocytes are activated and which antigen-presenting cell (apc) is critical for the regulation of this process. we have identified a unique population of apcs that is exclusively localized in the lamina propria. these apcs constitutively expressed the costimulatory molecule cd70 and had antigen-presenting functions. after oral infection of mice with listeria monocytogenes, proliferation and differentiation of antigen-specific t cells occurr ... | 2005 | 15937486 |
| immune responses of mice to vaccinia virus recombinants expressing either listeria monocytogenes partial listeriolysin or brucella abortus ribosomal l7/l12 protein. | the brucella abortus l7/l12 gene encoding ribosomal protein l7/l12 and the listeria monocytogenes partial hly gene encoding the protective region of the hemolysin (partial listeriolysin, pllo) were cloned into vaccinia virus by homologous recombination to produce recombinants wrl7/l12 and wrpllo, respectively. the ability of these recombinants to induce humoral, cell mediated and protective immune response in mice was assessed. although mice inoculated with wrl7/l12 recombinant produced antibodi ... | 2005 | 15941627 |
| in vivo modulation of t cell responses and protective immunity by tcr antagonism during infection. | infectious agents are known to express altered peptide ligands that antagonize t cells in vitro; however, direct evidence of tcr antagonism during infection is still lacking, and its importance in the context of infection remains to be established. in this study, we used a murine model of infection with recombinant listeria monocytogenes and addressed three issues that are critical for assessing the role of tcr antagonism in the modulation of the immune response. first, we demonstrated that the ... | 2005 | 15944303 |
| phosphatidylinositol-specific phospholipase c of bacillus anthracis down-modulates the immune response. | phosphatidylinositol-specific phospholipases (pi-plcs) are virulence factors produced by many pathogenic bacteria, including bacillus anthracis and listeria monocytogenes. bacillus pi-plc differs from listeria pi-plc in that it has strong activity for cleaving gpi-anchored proteins. treatment of murine dcs with bacillus, but not listeria, pi-plc inhibited dendritic cell (dc) activation by tlr ligands. infection of mice with listeria expressing b. anthracis pi-plc resulted in a reduced ag-specifi ... | 2005 | 15944308 |
| [investigation of electrophysical properties of listeria monocytogenes cells during the interaction with monoclonal antibodies]. | an electrooptical approach was used in studies of listeria monocytogenes-antibody binding. an electrooptical analyzer, which has been developed at the state research center for applied microbiology (obolensk, russia), was used as a basic instrument for electrooptical measurements. the analyzer consists of the following modules: a sample preparation module, a mixer, an ac field generator, an eo-flow cell, a microcontroller for transfer of liquid, a thermal system, an operator interface, and an im ... | 2005 | 15856991 |
| influence of bacteriocin-like substance, generation times, and genetic profiles of listeria innocua on the isolation of listeria monocytogenes. | inhibition of isolation of listeria monocytogenes by bacteriocin-like substance (bls)-producing listeria innocua after enrichment culture was investigated. when 26 l. monocytogenes strains were examined in combination with eight l. innocua strains using the spot on lawn method, 52/208 (25.0%) combinations showed the growth inhibition of l. monocytogenes. when two listeria species were cultured simultaneously in selective enrichment broth, inhibition of isolation of l. monocytogenes was observed ... | 2005 | 15857657 |
| evaluation of a chromogenic medium for identification and differentiation of listeria monocytogenes in selected foods. | listeria monocytogenes continues to be a threat to food safety in the united states despite a "zero tolerance" policy. when listeria species are identified by standard cultural methods, confirmation of l. monocytogenes takes days to complete. rapid'l.mono agar, developed by bio-rad laboratories, is a chromogenic medium that differentiates l. monocytogenes from other species of listeria by a simple color change reaction. differentiation is based on the specific detection of phosphatidylinositol p ... | 2005 | 15859079 |
| activity of three {beta}-lactams (ertapenem, meropenem and ampicillin) against intraphagocytic listeria monocytogenes and staphylococcus aureus. | assessment of the activity of three beta-lactams [ertapenem (a carbapenem with a prolonged half-life), meropenem and ampicillin] against intraphagocytic listeria monocytogenes and staphylococcus aureus. | 2005 | 15860552 |
| thermal resistance of salmonella enterica serotypes, listeria monocytogenes, and staphylococcus aureus in high solids liquid egg mixes. | decimal reduction times (d-values) were determined for salmonella enterica serotypes, listeria monocytogenes, and staphylococcus aureus in two high solids egg mixes designated a and b (water activity [a(w)] = 0.76 and 0.82; solids = 53.12 and 52.63%; ph = 5.09 and 5.29; viscosity = 183 and 119 centipoise/s, respectively) using a low-volume (0.06 ml) sealed glass capillary tube procedure. for salmonella, d-values ranged from 0.035 (70 degrees c) to 0.193 min (64 degrees c) in product a and from 0 ... | 2005 | 15830659 |
| development of a surface plasmon resonance-based immunoassay for listeria monocytogenes. | a polyclonal antibody was produced against internalin b (inlb)-enriched extract and used to develop an inhibition assay to detect listeria monocytogenes cells in solution using surface plasmon resonance. the gene sequence encoding for the inlb protein was cloned into a qiagen pqe-60 vector, expressed in escherichia coli, and purified by immobilized metal affinity chromatography. protein g-purified anti-inlb-enriched extract polyclonal antibody was incubated with various concentrations of l. mono ... | 2005 | 15830663 |
| predicting heat inactivation of listeria monocytogenes under nonisothermal treatments. | the aim of this study was to find a model that accurately predicts the heat inactivation of listeria monocytogenes (atcc 15313) at constantly rising heating rates (0.5 to 9 degrees c/min) in media of different ph values (4.0 to 7.4). survival curves of l. monocytogenes obtained under isothermal treatments at any temperature were nearly linear. estimations of survival curves under nonisothermal treatments obtained from heat resistance parameters of isothermal treatments adequately fit experimenta ... | 2005 | 15830664 |
| inactivation of barotolerant listeria monocytogenes in sausage by combination of high-pressure processing and food-grade additives. | food-grade additives were used to enhance the efficacy of high-pressure processing (hpp) against barotolerant listeria monocytogenes. three strains of l. monocytogenes (scott a, osy-8578, and osy-328) were compared for their sensitivity to hpp, nisin, tert-butylhydroquinone (tbhq), and their combination. inactivation of these strains was evaluated in 0.2 m sodium phosphate buffer (ph 7.0) and commercially sterile sausage. a cell suspension of l. monocytogenes in buffer (10(9) cfu/ml) was treated ... | 2005 | 15830665 |
| monochloramine versus sodium hypochlorite as antimicrobial agents for reducing populations of bacteria on broiler chicken carcasses. | studies were conducted to compare the effect of sodium hypochlorite (sh) versus monochloramine (mon) on bacterial populations associated with broiler chicken carcasses. in study 1, nominal populations (6.5 to 7.5 log cfu) of escherichia coli, listeria monocytogenes, pseudomonas fluorescens, salmonella serovars, shewanella putrefaciens, and staphylococcus aureus were exposed to sterilized chiller water (controls) or sterilized chiller water containing 50 ppm sh or mon. sh at 50 ppm eliminated all ... | 2005 | 15830667 |
| fate of listeria monocytogenes inoculated onto the surface of model turkey frankfurter pieces treated with zein coatings containing nisin, sodium diacetate, and sodium lactate at 4 degrees c. | the antimicrobial effects of zein coatings containing nisin, sodium lactate, and sodium diacetate against listeria monocytogenes on turkey frankfurters at 4 degrees c were determined. our objectives were to determine whether zein, nisin, lactate, and diacetate alone or in combination could control the growth of l. monocytogenes on full-fat turkey frankfurters at 4 degrees c and to determine whether lactate or diacetate had any synergistic effect on the activity of nisin. turkey frankfurter piece ... | 2005 | 15830684 |
| effectiveness of trisodium phosphate treatment against pathogenic and spoilage bacteria on poultry during refrigerated storage. | to determine the efficacy of trisodium phosphate (tsp) against pathogenic and spoilage bacteria on poultry and to assess the influence of the bacterial combination of inoculum on tsp effect, chicken legs were coinoculated with similar concentrations of a pathogenic (salmonella enterica ser. enteritidis or listeria monocytogenes) and a spoilage (pseudomonas fluorescens or brochothrix thermosphacta) bacteria. samples were dipped in tsp (12%, 15 min) or were not treated (control). microbiological a ... | 2005 | 15830686 |
| the role of sigmab in the stress response of gram-positive bacteria -- targets for food preservation and safety. | the alternative sigma factor sigmab modulates the stress response of several gram-positive bacteria, including bacillus subtilis and the food-borne human pathogens bacillus cereus, listeria monocytogenes and staphylococcus aureus. in all these bacteria, sigmab is responsible for the transcription of genes that can confer stress resistance to the vegetative cell. recent findings indicate that sigmab also plays an important role in antibiotic resistance, pathogenesis and cellular differentiation p ... | 2005 | 15831390 |
| fate of listeria monocytogenes in experimentally contaminated french sausages. | listeria monocytogenes has been recognized as one of the most important foodborne pathogens dealt with by the food. the bacterium has been found in every part along the pork processing industry from the slaughterhouse to the cutting room and the delicatessen factories. during the fermentation and drying of sausages, l. monocytogenes tends to decrease substantially. however, despite the various hurdles in the dry sausage manufacturing process, l. monocytogenes is able to survive and is detected i ... | 2005 | 15862881 |
| growth of listeria monocytogenes on iceberg lettuce and solid media. | the growth of pathogenic bacterium listeria monocytogenes on fresh-cut iceberg lettuce under constant temperatures was modelled in order to investigate microbial safety during distribution of this vegetable. we examined the effects of several incubation temperatures, ranging from 5 to 25 degrees c, on bacterial growth. these data were fitted to the baranyi model and the curves showed a high correlation coefficient at all temperature (r2 > 0.95). in addition, the native bacterial flora of the let ... | 2005 | 15862883 |
| pesticides as a source of microbial contamination of salad vegetables. | ten commercially available pesticides (insecticides, herbicides and fungicides), used during the production of vegetable produce, were examined as potential sources of microbial contaminants. as purchased, none of the pesticides showed the presence of viable microorganisms (< 5 cfu/ml). using an agar plate diffusion assay, they did not inhibit a range of bacteria of spoilage and public health significance on vegetable produce. after reconstitution in sterile water to their recommended concentrat ... | 2005 | 15862885 |
| beyond allergen avoidance: update on developing therapies for peanut allergy. | food allergy has emerged as a significant health problem. peanut allergy is a major cause of food-induced fatal and near fatal anaphylactic reactions, and the incidence in children is increasing. attempts to manage peanut allergy by strict avoidance are often unsuccessful. the purpose of this review is to highlight the most promising novel approaches for treating peanut allergy beyond allergen avoidance. | 2005 | 15864090 |
| [neurolisteriosis with acute myelitis]. | listeriosis is a bacterial infection with listeria monocytogenes mostly affecting immunocompromised patients. in every fourth case, the cns is involved, usually as meningoencephalitis. this case report of an immunocompetent woman represents the first one of neurolisteriosis initially presenting as cervical myelitis and progressing to supratentorial areas of the brain. diagnosis was based on universal polymerase chain reaction from a cortical brain biopsy, followed by sequencing of the amplified ... | 2005 | 15864516 |
| dietary (n-3) polyunsaturated fatty acids do not affect the in vivo development and function of listeria-specific cd4+ and cd8+ effector and memory/effector t cells in mice. | we previously reported that in a mouse model, a diet high in (n-3) pufa diminishes host survival following an infection from listeria monocytogenes, a gram-positive bacterial pathogen. in this study we investigated the impact of (n-3) pufa on the adaptive immune response to l. monocytogenes. balb/c mice were fed experimental diets either devoid of or rich in (n-3) pufa from fish oil for 4 wk and then infected with 10(6) acta-deficient l. monocytogenes. at 7 and 35 d postchallenge, effector and m ... | 2005 | 15867296 |
| potential role of diploscapter sp. strain lkc25, a bacterivorous nematode from soil, as a vector of food-borne pathogenic bacteria to preharvest fruits and vegetables. | diploscapter, a thermotolerant, free-living soil bacterial-feeding nematode commonly found in compost, sewage, and agricultural soil in the united states, was studied to determine its potential role as a vehicle of salmonella enterica serotype poona, enterohemorrhagic escherichia coli o157:h7, and listeria monocytogenes in contaminating preharvest fruits and vegetables. the ability of diploscapter sp. strain lkc25 to survive on agar media, in cow manure, and in composted turkey manure and to be ... | 2005 | 15870330 |
| growth of listeria monocytogenes in the guinea pig placenta and role of cell-to-cell spread in fetal infection. | listeria monocytogenes causes foodborne outbreaks that lead to infection in human and other mammalian fetuses. to elucidate the molecular and cellular mechanisms involved in transplacental transmission, we characterized placental-fetal infection in pregnant guinea pigs inoculated with wild-type (wt) or mutant l. monocytogenes strains. the wt strain increased in number in the placenta by >1000-fold during the first 24 h after inoculation--an increase that was unparalleled in other maternal organs ... | 2005 | 15871123 |
| microbiological aspects of the investigation that traced the 1998 outbreak of listeriosis in the united states to contaminated hot dogs and establishment of molecular subtyping-based surveillance for listeria monocytogenes in the pulsenet network. | a multistate outbreak of listeriosis occurred in the united states in 1998 with illness onset dates between august and december. the outbreak caused illness in 108 persons residing in 24 states and caused 14 deaths and four miscarriages or stillbirths. this outbreak was detected by public health officials in tennessee and new york who observed significant increases over expected listeriosis cases in their states. subsequently, the centers for disease control and prevention (cdc) began laboratory ... | 2005 | 15872265 |
| listeria-associated arthritis in a patient undergoing etanercept therapy: case report and review of the literature. | listeriosis can be a cause of infectious arthritis. here, we present a case of articular listeriosis in a patient with rheumatoid arthritis receiving treatment with etanercept, a tumor necrosis factor antagonist. we review the literature of articular listeriosis and discuss the role of tumor necrosis factor blockade in precipitating listeriosis. | 2005 | 15872306 |
| listeria meningitis in transplant recipients. | meningitis is a rare complication following organ and stem-cell transplantation and can be caused by a variety of microorganisms. | 2005 | 15875764 |
| tracing listeria monocytogenes isolates from cold-smoked salmon and its processing environment in iceland using pulsed-field gel electrophoresis. | listeria spp. and listeria monocytogenes contamination of cold-smoked salmon (n=125) and its processing environment (n=522) were evaluated during surveys conducted in 1997-1998 and 2001 as well as in samples of final products analysed in 2001. the overall frequencies of listeria spp. and l. monocytogenes in samples from all sources were 15.1% and 11.3%, respectively, but the incidence of l. monocytogenes in cold-smoked salmon final products was only 4%. a total of 201 l. monocytogenes isolates w ... | 2005 | 15878405 |
| effect of prior growth temperature, type of enrichment medium, and temperature and time of storage on recovery of listeria monocytogenes following high pressure processing of milk. | a five-isolate cocktail of listeria monocytogenes (10(3) cfu/ml in skim or whole raw milk) was subjected to 450 mpa for 900 s or 600 mpa for 90 s. the effects of prior growth temperature, type of milk (skim vs. whole), type of recovery-enrichment media (optimized penn state university [opsu] broth, listeria enrichment broth [leb], buffered leb [bleb], modified bleb [mbleb], and milk), storage temperature and storage time on the recovery of l. monocytogenes were examined. optimized psu broth sign ... | 2005 | 15878406 |
| rapid clearance of a recombinant salmonella vaccine carrier prevents enhanced antigen-specific cd8 t-cell responses after oral boost immunizations. | the type iii secretion system of salmonella enterica serovar typhimurium can be used to target heterologous antigens directly into the cytosol of antigen-presenting cells. our laboratory has previously reported that the single oral immunization of mice with a recombinant salmonella strain expressing the translocated yersinia outer protein e fused to the immunodominant antigen p60 from listeria monocytogenes results in the efficient induction of p60-specific cd8 t cells and confers protection aga ... | 2005 | 15878680 |
| internalin-expressing lactococcus lactis is able to invade small intestine of guinea pigs and deliver dna into mammalian epithelial cells. | the use of the food-grade bacterium lactococcus lactis as antigen delivery vehicle at the mucosal level is an attractive vaccination strategy intensively explored during the last decade. in this study, we developed l. lactis strains which could be used as a dna delivery vector to combine both advantages of mucosal delivery and of dna vaccination. to render lactococci capable of invading epithelial cells, the listeria monocytogenes inla gene was cloned and expressed in l. lactis under transcripti ... | 2005 | 15878681 |
| cutting edge: re-evaluating the in vivo cytokine responses of cd8+ t cells during primary and secondary viral infections. | virus-specific cd8(+) t cells produce ifn-gamma after ag contact and, in the absence of this cytokine, the host often cannot eradicate infection. however, our ability to identify cells that are actively expressing this critical effector function in vivo is limited, because the protein is rapidly secreted. in this study, we describe a simple approach that circumvents the need for ex vivo ag stimulation and allows the enumeration of cd8(+) t cells that are actively synthesizing ifn-gamma in vivo d ... | 2005 | 15879085 |
| large-scale quantitative analysis of sources of variation in the actin polymerization-based movement of listeria monocytogenes. | during the actin polymerization-based movement of listeria monocytogenes, individual bacteria are rapidly propelled through the host cell cytoplasm by the growth of a filamentous actin tail. the rate of propulsion varies significantly among individuals and over time. to study this variation, we used a high-throughput tracking technique to record the movement of a large number (approximately 7900) of bacteria in xenopus frog egg extract. most bacteria (70%) appeared to maintain an individual char ... | 2005 | 15879472 |
| rat hepatocyte invasion by listeria monocytogenes and analysis of tnf-alpha role in apoptosis. | listeria monocytogenes, etiological agent of severe human foodborne infection, uses sophisticated mechanisms of entry into host cytoplasm and manipulation of the cellular cytoskeleton, resulting in cell death. the host cells and bacteria interaction may result in cytokine production as tumor necrosis factor (tnf) alpha. hepatocytes have potential to produce pro-inflammatory cytokines as tnf-alpha when invaded by bacteria. in the present work we showed the behavior of hepatocytes invaded by l. mo ... | 2005 | 15880217 |
| [influence of gaseous metabolites of soil bacteria on the multiplication of listeria monocytogenes and yersinia pseudotuberculosis]. | the influence of gaseous metabolites of saprophytic soil bacteria on the growth and multiplication of l. monocytogenes and y. pseudotuberculosis was studied. the study revealed that all cultures under study exhibited selectively both inhibiting and stimulating action on the multiplication of test cultures. bacteria of the genera pseudomonas and acinetobacter showed the highest inhibiting activitywith respect to the test cultures. volatile metabolites of bacteria of the genus aeromonas showed the ... | 2005 | 15881930 |
| understanding how listeria monocytogenes targets and crosses host barriers. | human listeriosis is caused by the gram-positive bacterium listeria monocytogenes. in humans, this pathogen has the ability to cross the intestinal, placental and blood-brain barriers, leading to gastroenteritis, maternofetal infections and meningoencephalitis, respectively. the entry of l. monocytogenes into cultured human epithelial cells is mediated by the interaction of an l. monocytogenes surface protein, internalin, with its human receptor, e-cadherin. the internalin-e-cadherin interaction ... | 2005 | 15882192 |
| the listeria protein internalin b mimics hepatocyte growth factor-induced receptor trafficking. | increased hepatocyte growth factor receptor (hgfr) signaling correlates closely with neoplastic invasion and metastatic potential of many human cancers. hepatocyte growth factor receptor signaling is initiated by binding the physiological ligand hgf or the internalin b (inlb) protein of listeria monocytogenes. subsequent degradation of endocytosed hgfr terminates receptor signaling. previously reported discrepancies in inlb and hgf-induced hgfr signaling could reflect differences in receptor int ... | 2005 | 15882443 |
| apoptotic death of listeria monocytogenes-infected human macrophages induced by lactoferricin b, a bovine lactoferrin-derived peptide. | listeria monocytogenes, an intracellular facultative food-borne pathogen, was reported to induce apoptosis in vitro and in vivo in a variety of cell types with the exception of murine macrophages. these cells represent the predominant compartment of bacterial multiplication and die as a result of necrosis. in this study we showed that human non-activated and ifn-gamma-activated macrophagic-like (thp-1) cells infected with l. monocytogenes, mainly die by necrosis rather than by an apoptotic proce ... | 2005 | 15888254 |
| diagnosis of partially treated culture-negative bacterial meningitis using 16s rrna universal primers and restriction endonuclease digestion. | cerebrospinal fluid (csf) obtained from patients with partially treated and culture-negative meningitis was subjected to pcr using 16s rdna universal primers followed by restriction endonuclease digestion. in all, 43 patients and 7 controls were enrolled in this study. twenty-one meningitic samples were positive by pcr. mycobacterium tuberculosis was the causative agent in seven cases followed by haemophilus influenzae (four), streptococcus pneumoniae (two), listeria monocytogenes (one), escheri ... | 2005 | 15888461 |
| dendritic cells maximize the memory cd8 t cell response to infection. | costimulatory signals from dendritic cells (dcs) are required for naive t cells to respond to antigenic stimulation. to what extent dcs reactivate memory t cells during recall responses is not known. here, an in vivo depletion system has been used to analyze the role of dcs in reactivating cd8 memory t cells during recall responses to three different microbial infections. we show a profound decrease in the numbers of responding memory cd8 t cells in both lymphoid and nonlymphoid tissues during t ... | 2005 | 15894274 |
| enhanced toll-like receptor responses in the absence of signaling adaptor dap12. | dap12 is a signaling adaptor containing an immunoreceptor tyrosine-based activation motif (itam) that pairs with receptors on myeloid cells and natural killer cells. we examine here the responses of mice lacking dap12 to stimulation through toll-like receptors (tlrs). unexpectedly, dap12-deficient macrophages produced higher concentrations of inflammatory cytokines in response to a variety of pathogenic stimuli. additionally, macrophages deficient in spleen tyrosine kinase (syk), which signals d ... | 2005 | 15895090 |
| comprehensive survey of pasteurized fluid milk produced in the united states reveals a low prevalence of listeria monocytogenes. | a comprehensive survey was undertaken to generate contemporary data on the prevalence of listeria monocytogenes in pasteurized fluid milk produced in the united states. samples (5,519) near the sell-by expiration date were purchased at retail outlets over a 5-week period and analyzed for presence of l. monocytogenes. products consisted of whole milk, nonfat milk, and chocolate milk packaged in gallon, half gallon, quart, pint, and half-pint containers. samples were collected from both large and ... | 2005 | 15895729 |
| distribution of listeria monocytogenes subtypes within a poultry further processing plant. | samples from environmental sites and raw product in a chicken further processing plant were collected every 6 weeks for 12 months. each sample site was examined before and after a complete production shift. all samples were examined for the presence of listeria monocytogenes, which was detected in floor drains on the raw product side of the plant preoperation and in drains on both raw and cooked sides following 8 h of processing operation. l. monocytogenes also was detected in raw product and on ... | 2005 | 15895730 |
| efficacy of electrolyzed water in inactivating salmonella enteritidis and listeria monocytogenes on shell eggs. | the efficacy of acidic electrolyzed (eo) water produced at three levels of total available chlorine (16, 41, and 77 mg/ liter) and chlorinated water with 45 and 200 mg/liter of residual chlorine was investigated for inactivating salmonella enteritidis and listeria monocytogenes on shell eggs. an increasing reduction in listeria population was observed with increasing chlorine concentration from 16 to 77 mg/liter and treatment time from 1 to 5 min, resulting in a maximal reduction of 3.70 log cfu ... | 2005 | 15895731 |
| postprocessing antimicrobial treatments to control listeria monocytogenes in commercial vacuum-packaged bologna and ham stored at 10 degrees c. | the antilisterial effect of chemical dipping solutions on commercial bologna and ham slices, inoculated (3 to 4 log cfu/ cm2) after processing, was evaluated during storage in vacuum packages at 10 degrees c. samples were inoculated with a 10-strain composite of listeria monocytogenes and subsequently immersed (25+/-2 degrees c) for 2 min in 2.5% acetic acid (aa), 2.5% lactic acid (la), 5% potassium benzoate (pb), or 0.5% nisaplin (commercial form of nisin, equivalent to 5,000 iu/ml of nisin) so ... | 2005 | 15895732 |
| prewashing with acidified sodium chlorite reduces pathogenic bacteria in lightly fermented chinese cabbage. | efficacy of prewashing with acidified sodium chlorite (asc) for the sanitation of lightly fermented chinese cabbage was evaluated. the population of the natural microflora on the cabbage leaves was reduced about 2.0 log cfu/g just after washing with asc, a significant reduction compared with the control distilled water wash (p < or = 0.05). in the control experiment, viable aerobic bacteria increased gradually when incubated at 10 degrees c; however, asc-washed cabbage maintained a lower microbi ... | 2005 | 15895733 |
| antimicrobial activity of nisin adsorbed to surfaces commonly used in the food industry. | the adsorption isotherms of nisin to three food contact surfaces, stainless steel, polyethyleneterephthalate (pet), and rubber at 8, 25, 40, and 60 degrees c, were calculated. for all surfaces, the increase in temperature led to a decrease in the affinity between nisin and the surface. the rubber adsorbed a higher amount of nisin (0.697 microg/cm2) in comparison with pet (0.665 microg/cm2) and stainless steel (0.396 microg/cm2). adsorption of nisin to the stainless steel surface described l-2 ty ... | 2005 | 15895735 |
| bacteriophage control of foodborne bacteriat. | bacteriophages are measurable components of the natural microflora in the food production continuum from the farm to the retail outlet. phages are remarkably stable in these environments and are readily recovered from soil, sewage, water, farm and processing plant effluents, feces, and retail foods. purified high-titer phage lysates have been used for the species-specific control of bacteria during the pre- and postharvest phases of food production and storage. for example, the inhibition of the ... | 2005 | 15895751 |
| unity in organisation and regulation of catabolic operons in lactobacillus plantarum, lactococcus lactis and listeria monocytogenes. | global regulatory circuits together with more specific local regulators play a notable role when cells are adapting to environmental changes. lactococcus lactis is a lactic acid bacterium abundant in nature fermenting most mono- and disaccharides. comparative genomics analysis of the operons encoding the proteins and enzymes crucial for catabolism of lactose, maltose and threhalose revealed an obvious unity in operon organisation . the local regulator of each operon was located in a divergent tr ... | 2005 | 15900965 |
| a multidomain fusion protein in listeria monocytogenes catalyzes the two primary activities for glutathione biosynthesis. | glutathione is the predominant low-molecular-weight peptide thiol present in living organisms and plays a key role in protecting cells against oxygen toxicity. until now, glutathione synthesis was thought to occur solely through the consecutive action of two physically separate enzymes, gamma-glutamylcysteine ligase and glutathione synthetase. in this report we demonstrate that listeria monocytogenes contains a novel multidomain protein (termed gshf) that carries out complete synthesis of glutat ... | 2005 | 15901709 |
| activated antigen-presenting cells select and present chemically modified peptides recognized by unique cd4 t cells. | cd4 t cells recognized posttranslationally modified peptides of the protein hen egg-white lysozyme (hel), consisting of nitration of tyrosines and modifications of tryptophans in the t cell contact residues of the peptides. t cells were directed against modifications of a chemically dominant hel peptide as well as a minor hel peptide, bound to the class ii histocompatibility molecule i-a(k). the modified peptides were generated in vivo after immunization with native hel molecules or were generat ... | 2005 | 15901898 |
| dynamic regulation of ifn-gamma signaling in antigen-specific cd8+ t cells responding to infection. | ifn-gamma plays a critical role in the cd8(+) t cell response to infection, but when and if this cytokine directly signals cd8(+) t cells during an immune response is unknown. we show that naive ag-specific cd8(+) t cells receive ifn-gamma signals within 12 h after in vivo infection with listeria monocytogenes and then become unresponsive to ifn-gamma throughout the ensuing ag-driven expansion phase. ag-specific cd8(+) t cells regain partial ifn-gamma responsiveness throughout the contraction ph ... | 2005 | 15905520 |
| the role of protein kinase a anchoring via the rii alpha regulatory subunit in the murine immune system. | intracellular camp may inhibit t cell activation and proliferation via activation of the camp-dependent protein kinase, pka. pka signaling is maintained through interactions of the regulatory subunit with a-kinase anchoring proteins (akaps). we demonstrated that t cells contain akaps and now ask whether pka anchoring to akaps via the riialpha regulatory subunit is necessary for camp-mediated inhibition of t cell activation. we studied the immune systems of mice lacking the riialpha regulatory su ... | 2005 | 15905526 |
| the antiapoptotic protein bcl-xl is dispensable for the development of effector and memory t lymphocytes. | the antiapoptotic protein bcl-x(l) is induced in activated t lymphocytes upon costimulation through cd28, 4-1bb, and ox40. bcl-x(l) is also highly enriched in memory t lymphocytes. based on this body of evidence, it was thought that bcl-x(l) plays an essential role in the generation of effector and memory t lymphocytes. we report that mice with a conditional deletion of bcl-x in t lymphocytes develop a normal cd8(+) t cell response to listeria monocytogenes infection. furthermore, bcl-x conditio ... | 2005 | 15905539 |
| risk factors for intramammary infections and relationship with somatic-cell counts in italian dairy goats. | routine examination of milk was performed on five herds of lactating goats in northern italy as part of a milk quality-monitoring program in the year 2000. as part of the study, aseptic samples of foremilk were collected monthly from both half udders during the entire lactation for 305 goats, resulting in a total of 4571 samples. the samples were tested with cytological and bacteriological analyses to evaluate the relationship between mammary infections and somatic-cell count (scc; fossomatic (t ... | 2005 | 15907567 |
| cationic microparticles consisting of poly(lactide-co-glycolide) and polyethylenimine as carriers systems for parental dna vaccination. | cationic microparticles for dna adsorption were formulated by blending poly(lactide-co-glycolide) (plga) (50:50), with different cationic agents, either pei 25 kda (polyethylenimine) or ctab (cetyl-trimethyl-ammonium-bromide). the aim was to create adjuvant delivery systems increasing the efficiency of dna vaccines. microparticles formulated with 10% pei exhibited a highly positive zeta-potential, small particle sizes, in contrast to particles prepared with ctab, which revealed highly aggregated ... | 2005 | 15907586 |
| contribution of nitric oxide to cpg-mediated protection against listeria monocytogenes. | immunostimulatory cpg oligodeoxynucleotides (odn) improve host resistance to listeriae. cpg odn trigger immune cells to produce gamma interferon and "prime" host cells to secrete nitric oxide in response to bacterial exposure. cpg treatment does not protect inducible nitric oxide synthase 2 knockout mice, indicating that no is critical to cpg-mediated protection against listeriae. | 2005 | 15908417 |
| analysis of the prevalence of tetracycline resistance genes in clinical isolates of enterococcus faecalis and enterococcus faecium in a japanese hospital. | prevalence of seven tetracycline resistance (tc(r)) genes--tet(l), tet(m), tet(k), tet(o), tet(s), tet(t), and tet(u)--which are known to be distributed to gram-positive cocci was analyzed for 224 enterococcus faecalis and 46 enterococcus faecium clinical isolates obtained in a japanese hospital. any of the tc(r) genes was detected in 75.9% of all the enterococcal strains. the tet(m) was detected at highest rates in both e. faecalis (75.0%) and e. faecium (69.6%), followed by tet(l), which was h ... | 2005 | 15910229 |
| characterization of anti-self cd8 t-cell responses stimulated by recombinant listeria monocytogenes expressing the melanoma antigen trp-2. | a potential approach to activate tumor-specific t cells is to use live bacterial vectors to deliver appropriate antigens in a highly immunostimulatory context. we constructed a recombinant strain of listeria monocytogenes (rlm) expressing murine tyrosinase-related protein-2 (trp-2), a nonmutated melanocyte-derived differentiation antigen highly expressed in melanomas. immunization of c57bl/6 mice with this rlm strain efficiently primed cd8 t cells to recognize the mhc class i-restricted trp-2180 ... | 2005 | 15913853 |
| uncaria tomentosa extract increases the number of myeloid progenitor cells in the bone marrow of mice infected with listeria monocytogenes. | in this study, we demonstrated that uncaria tomentosa extract (ute) protects mice from a lethal dose of listeria monocytogenes when administered prophylactically at 50, 100, 150 and 200 mg/kg for 7 days, with survival rates up to 35%. these doses also prevented the myelosuppression and the splenomegaly caused by a sublethal infection with l. monocytogenes, due to increased numbers of granulocyte-macrophage progenitors (cfu-gm) in the bone marrow. non-infected mice treated with 100 mg/kg ute also ... | 2005 | 15914328 |
| gastrointestinal phase of listeria monocytogenes infection. | 2005 | 15916648 | |
| proteomic and microscopic analysis of biofilms formed by listeria monocytogenes 568. | biofilm formation may be important in the colonization of the food-processing environment by the food-borne pathogen listeria monocytogenes. listeria monocytogenes 568 formed adherent multicellular layers on a variety of test surfaces following growth at 37 degrees c with multiple transfers of the test surface into fresh medium. microscopic examination of these adherent layers suggest that the cells were surrounded by extracellular material. the presence of a carbohydrate containing extracellula ... | 2005 | 15920617 |
| [a new complex approaches for the identification of listeria isolated during production of fermented sausages]. | distribution l. monocytogenes and other listeria spp. in raw meat and during manufacturing of fermented meat products is investigated. the high contamination of raw materials and semi finished foods--in 36.5% of samples, ready-to-eat sausages--31.8% by listeria spp. is established. detection l. monocytogenes in 9.7% cases from the surfaces of equipment indicates the intensive circulation of listeriosis agents on meat plants. for identification 49 isolated strains the approach providing applicati ... | 2005 | 15921204 |
| thalidomide enhances both primary and secondary host resistances to listeria monocytogenes infection by a neutrophil-related mechanism in female b6c3f1 mice. | previously, we have reported that thalidomide can modulate the immune responses in female b6c3f1 mice. furthermore, thalidomide immunomodulation increased primary host resistance to intravenously infected listeria monocytogenes. the present study was intended to evaluate the mechanisms underlying the enhanced host resistance to l. monocytogenes by focusing on the neutrophils. female b6c3f1 mice were treated intraperitoneally with thalidomide (100 mg/kg) for 15 days. exposure to thalidomide incre ... | 2005 | 15921716 |
| foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. | milk and products derived from milk of dairy cows can harbor a variety of microorganisms and can be important sources of foodborne pathogens. the presence of foodborne pathogens in milk is due to direct contact with contaminated sources in the dairy farm environment and to excretion from the udder of an infected animal. most milk is pasteurized, so why should the dairy industry be concerned about the microbial quality of bulk tank milk? there are several valid reasons, including (1) outbreaks of ... | 2005 | 15992306 |
| effect of the implementation of haccp on the microbiological quality of meals at a university restaurant. | a study was conducted to evaluate the microbiological quality, including total mesophilic counts and markers of bacteriological hygiene, as indicator of food safety of three categories of the most consumed meals in a university restaurant, before and after implementation of the haccp system and personnel training. cold gastronomy products, cooked warm-served products, and cooked cold-served products were tested for bacterial contamination. throughout the experiment, 894 samples were examined for ... | 2005 | 15992308 |
| antibiotic resistance and antibacterial activity in heterotrophic bacteria of mineral water origin. | antibiotic resistance and antibacterial activity were determined on heterotrophic bacteria isolated from mineral waters. of the 120 isolates pseudomonas spp. (55.8%) was the predominant group followed by acinetobacter spp. (14.17%), flavobacterium spp. (10.83%), achromobacter spp. (10%), burkholderia cepacia (3.3%), agrobacterium/radiobacter (2.5%), moraxella spp. (1.7%), aeromonas hydrophila (1.7%). over 80% of the isolates were resistant to one or more antibiotics and the highest resistance wa ... | 2005 | 15993695 |
| prevalence and location of listeria monocytogenes in farmed rainbow trout. | a total of 510 rainbow trout originating from fish farms in lakes and sea areas around finland were studied for the presence of listeria monocytogenes. samples were studied as pools from five fish. gill, viscera, and skin from the pooled samples were analysed separately. the individual samples were analysed later if the pooled sample was found to be listeria positive. the prevalence of listeria spp. and l. monocytogenes in pooled unprocessed fresh rainbow trout was on average 35.0% and 14.6%, re ... | 2005 | 15993967 |
| characterization of listeria monocytogenes isolates from food animal clinical cases: pfge pattern similarity to strains from human listeriosis cases. | twenty-one isolates of listeria monocytogenes from food animal clinical cases that involved meningitis or meningoencephalitis, encephalitis, mastitis and abortion were characterized by serotyping and pulsed-field gel electrophoresis (pfge) in order to improve our understanding of the genetic links between individual strains and strains recovered from human listeriosis cases. results showed that five of the isolates were serotype 1/2a, six were 1/2b, nine were 4b, and one was untypeable. a caprin ... | 2005 | 16000241 |
| suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of listeria monocytogenes from genomic dna. | listeria monocytogenes is a significant cause of food-borne disease and mortality; therefore, epidemiological investigations of this pathogen require subtyping methods that are rapid, discriminatory, and reproducible. although conventional microarray subtyping analysis has been shown to be both high resolution and genetically informative, it is still relatively low throughput and technically challenging. suspension microarray technology eliminates the technical issues associated with planar micr ... | 2005 | 16000444 |
| definition of genetically distinct attenuation mechanisms in naturally virulence-attenuated listeria monocytogenes by comparative cell culture and molecular characterization. | listeria monocytogenes is a foodborne pathogen able to cause serious disease in humans and animals. not all isolates are equally pathogenic, however, and several isolates have been characterized as naturally virulence attenuated. we sought to identify the genetic basis of natural virulence attenuation using cell culture assays and molecular techniques. by comparing the phenotypes of naturally virulence-attenuated isolates to those of defined virulence gene mutants in plaque, cytotoxicity, and he ... | 2005 | 16000803 |
| functional transfer of eukaryotic expression plasmids to mammalian cells by listeria monocytogenes: a mechanistic approach. | cystic fibrosis (cf) is one of the most common monogenic disorders in the caucasian population. gene therapy for cf is principally feasible and bacterial transfer systems might provide novel possibilities for therapy. however, transfection efficiencies are low and need to be improved. thus, more detailed understanding of the dna transfer mechanism is necessary to systematically eliminate these restrictions. | 2005 | 16001391 |